WO2016078088A1 - Utilisation d'huile sacha inchi dans la régulation de la flore du tractus intestinal d'animaux - Google Patents

Utilisation d'huile sacha inchi dans la régulation de la flore du tractus intestinal d'animaux Download PDF

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Publication number
WO2016078088A1
WO2016078088A1 PCT/CN2014/091922 CN2014091922W WO2016078088A1 WO 2016078088 A1 WO2016078088 A1 WO 2016078088A1 CN 2014091922 W CN2014091922 W CN 2014091922W WO 2016078088 A1 WO2016078088 A1 WO 2016078088A1
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weight
parts
oil
group
fruit oil
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PCT/CN2014/091922
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English (en)
Chinese (zh)
Inventor
朱江阳
张海峰
丁淑娟
朱倩
朱端清
张珊珊
过敏
张亮波
张耕耘
金桃
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深圳华大基因科技有限公司
深圳华大农业与循环经济科技有限公司
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Priority to PCT/CN2014/091922 priority Critical patent/WO2016078088A1/fr
Priority to CN201480083554.0A priority patent/CN106998723A/zh
Publication of WO2016078088A1 publication Critical patent/WO2016078088A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils

Definitions

  • the present invention relates to a novel use of cane fruit oil, and in particular to the use of candied fruit oil in regulating intestinal flora of an animal. More specifically, the present invention relates to the use of the cane fruit oil for regulating the intestinal flora of an animal, the use of cane fruit oil for the preparation of a food flavoring agent, the medicinal food flavoring agent, and a method for regulating the intestinal flora of an animal.
  • Plukenetia volubilis L., Inca Peanut, sacha inchi is a large vine plant, also known as Inca, Inca peanut, South American oil vine, Star oil vine.
  • the perennial oil plant, native to the Amazonian rainforest of South America, was originally transformed from the Incas to the wild, and has been used by local indigenous people for thousands of years in the Inca region of South America. When planted in the same year, the fruit can be hung in the same year.
  • the seeds are rich in oil, protein, amino acids, vitamin A, vitamin E and some other trace elements.
  • Meito fruit oil contains extremely rich unsaturated fatty acids ( ⁇ -3, ⁇ -6, ⁇ -9), as well as vitamins A, E and protein, and the content of three unsaturated fatty acids is as high as 92% or more. It is rich in human essential fatty acid ⁇ -linolenic acid. It is the mother of omega-3 unsaturated fatty acids and is the most basic and primitive substance in the process of life evolution. As the core material of life, ⁇ -linolenic acid is one of the important components of human tissue cells. It participates in the synthesis and metabolism of phospholipids in the human body and transforms into the vital activity factors DHA and EPA, which are essential for ⁇ -linolenic acid in human body. At the time, various diseases may be caused by low immunity.
  • the present invention is directed to solving at least some of the above technical problems or at least providing a useful commercial choice. To this end, it is an object of the present invention to provide a new use of the cane fruit oil.
  • the inventors have found that the fruit of the cane has a regulating effect on the intestinal flora of the human body, and has the effects of lowering blood sugar, lowering blood fat, and losing weight, and it is safe to eat the cane fruit and oil rich in polyunsaturated fatty acids. It can be served coldly or fried.
  • the invention provides the use of candied fruit oil for regulating intestinal flora of an animal.
  • the cane fruit oil can significantly increase the number of probiotics in the intestinal tract of the animal, thereby significantly regulating the intestinal flora of the animal, thereby exerting blood sugar lowering, lowering blood fat, and losing weight through the action of the intestinal flora. The effect.
  • the provision of the cane fruit oil to the animal can effectively regulate the intestinal flora, thereby preventing or treating obesity, diabetes, and hyperlipidemia.
  • the animal species referred to in the expression "animal” in the expression “the use of the cane fruit oil in regulating the intestinal flora of the animal” used herein is not particularly limited.
  • the animal may be any animal having an intestinal organ, preferably a mammal, more preferably a rat, a mouse, a human, and most preferably a human.
  • prevention or treatment as used herein mainly refers to the prevention or adjuvant treatment of obesity, diabetes, and hyperlipidemia.
  • the administration of the cane fruit oil does not have any side effects, and thus the dosage of the cane fruit oil is not particularly limited as long as the amount of the probiotic bacteria in the intestinal tract of the administered animal is improved and the intestinal bacteria of the animal are effectively regulated.
  • Group can be.
  • the cane fruit oil is supplied to the animal at a dose of not less than 80 mg/kg body weight.
  • the cane oil is supplied to the animal at a dose of not less than 300 mg/kg body weight.
  • the preparation of the cane fruit oil is carried out by a low temperature cold pressing method.
  • the preparation process of the cane fruit oil of the invention is as follows: after the mature Mino fruit is harvested, the machine (or artificial) is used to break the flap, the shell is removed, and the black seed is obtained, and then the black shell is obtained. Seed kernel), the finished product of Meito fruit oil is obtained by pulverization, low temperature cold pressing, filtration and impurity removal.
  • the pressing temperature should not exceed 50 ° C
  • the pressing time is 30-90 min
  • the pressing pressure is 30-80 MPa. After the oil is pressed, the finished product can be obtained by fine filtration.
  • the present invention provides the use of the cane fruit oil for preparing a food flavoring agent based on the above-described effect of the artichoke oil in regulating the intestinal flora of the animal, the food seasoning
  • the agent is used to regulate the intestinal flora of animals.
  • the above food flavoring agent can be used to regulate the intestinal flora of an animal, and thereby can exert effects such as weight loss, blood sugar lowering, and blood fat lowering by the action of the intestinal flora.
  • the food flavoring agent has the effects of lowering blood sugar, lowering blood fat, and losing weight.
  • the invention also provides a food flavoring agent.
  • the food flavoring comprises meringue oil.
  • the inventors have surprisingly found that the food flavoring agent of the present invention can significantly increase the number of probiotic bacteria in the intestinal tract of an animal, regulate the intestinal flora of the animal, and thereby effectively prevent or treat obesity, diabetes, and hyperlipidemia through the action of the intestinal flora.
  • the form of providing the food flavoring agent of the present invention is not particularly limited.
  • the food flavoring agent is at least one selected from the group consisting of jam, salad dressing, flavored blend oil, chili sauce, peanut butter, and sesame paste.
  • the jam comprises: 10 to 30 parts by weight of white granulated sugar and a thickener, 30 to 40 parts by weight of syrup, 40 to 60 parts by weight of fruit, and 10 parts by weight of candied fruit oil;
  • the salad dressing comprises: 20 to 35 parts by weight of soybean salad oil, 30 to 40 parts by weight of meto fruit oil, and 1.75 to 4 weight of inulin. Parts, egg liquid 5 to 15 parts by weight, xanthan gum powder 0.25 to 0.5 parts by weight, salt 0.03 to 0.05 parts by weight, mustard oil 0.03 to 0.05 parts by weight, MSG 0.001 to 0.002 parts by weight, purified water 20 to 30.0 parts by weight ;
  • the flavor blending oil comprises: 50.0-65.0 parts by weight of sesame oil or olive oil, and 35.0-50.0 parts by weight of the cane fruit oil;
  • the chili sauce comprises: 100 parts by weight of fresh pepper, 20 to 35 parts by weight of edible oil, 20 to 35 parts by weight of metopine oil, 25 to 60 parts by weight of vinegar, 8 to 20 parts by weight of salt, and 0 to 30 parts by weight of garlic. Parts, 0 to 30 parts by weight of ginger, 0 to 15 parts by weight of chicken essence and 0 to 10 parts by weight of allspice;
  • the peanut butter comprises: 120 to 200 parts by weight of fresh peanuts, 30 to 50 parts by weight of metopine oil, 3 to 15 parts by weight of salt, and 5 to 10 parts by weight of white sugar;
  • the sesame paste contains 60 to 85 parts by weight of sesame, 5 to 15 parts by weight of salt, 10 to 15 parts by weight of meto juice, 1 to 2 parts by weight of sugar, 6 to 15 parts by weight of water, and 2 to 3 parts by weight of chicken.
  • the food flavoring agent of the present invention has the effects of lowering blood sugar, lowering blood fat, and losing weight.
  • the animal taking the food flavoring agent of the present invention has an obvious increase in the number of probiotics in the intestine, and a significant decrease in body weight, blood sugar and blood lipids.
  • the food flavoring agent of the present invention does not have any side effects, and thus the dosage of the food flavoring agent is not particularly limited as long as the amount of probiotic bacteria in the intestinal tract of the test animal can be increased and the intestinal bacteria of the animal can be effectively regulated. Group can be.
  • the food flavoring agent is provided to the animal at a dose of not less than 80 mg of cane oil/kg body weight.
  • the cane oil is supplied to the animal at a dose of not less than 300 mg/kg body weight.
  • the present invention also provides a method of regulating an intestinal flora of an animal.
  • the method is to provide the animal with the cane fruit oil or the food flavoring agent as described above.
  • the method of the invention can significantly increase the number of probiotics in the intestine of the animal to be administered, effectively regulate the intestinal flora of the animal, and thereby effectively prevent or treat obesity and diabetes through the action of the intestinal flora.
  • the invention applies the Meito fruit to the daily food flavoring agent, can regulate the intestinal flora to lower the blood sugar, lower the lipid-lowering effect, and achieve the effect of improving the skin condition, whitening and freckle by conditioning the human body function, and has the promotion.
  • Preparation solution 10 parts by weight of white granulated sugar is mixed with a thickener, added to 60 parts by weight of water for blending and heating, and after the temperature reaches 95 ° C, 30 parts by weight of syrup is added, stirred uniformly, and decomposed and filtered. Prepared into a sugar solution;
  • Tanning add 40 parts by weight of apple and a small amount of acidity regulator, food flavor, preservative to the prepared sugar liquid, stir evenly, and make applesauce under high temperature;
  • Blending After cooling the jam, add the Minoberry oil in a proportion of 10 parts by weight, and stir evenly;
  • Tanning adding 50 parts by weight of kiwifruit and a small amount of acidity regulator, food flavor, preservative to the prepared sugar liquid, stirring uniformly; kiwi sauce is prepared by high temperature mash;
  • Blending After cooling the jam, add the Minoberry oil in a proportion of 10 parts by weight, and stir evenly;
  • Tanning adding 60 parts by weight of Sydney and a small amount of acidity regulator, food flavor, preservative to the prepared sugar liquid, stirring evenly;
  • Blending After cooling the jam, add the Minoberry oil in a proportion of 10 parts by weight, and stir evenly;
  • Raw material 30 parts by weight of soybean salad oil, 30 parts by weight of metoceroil, 1.75 parts by weight of inulin, 10 parts by weight of egg liquid (made of fresh eggs), 0.25 parts by weight of xanthan gum powder, 0.03 parts by weight of salt, and mustard 0.03 parts by weight of oil, 0.001 parts by weight of MSG, and 27.94 parts by weight of purified water.
  • Raw materials 20 parts by weight of soybean salad oil, 40 parts by weight of metoceroil, 4 parts by weight of inulin, 13 parts by weight of egg liquid (made of fresh eggs), 0.35 parts by weight of xanthan gum powder, 0.05 parts by weight of salt, and mustard 0.05 parts by weight of oil, 0.002 parts by weight of MSG, and 20 parts by weight of purified water.
  • Raw material 50.0 parts by weight of olive oil and 50.0 parts by weight of metopine oil.
  • Raw material 65.0 parts by weight of sesame oil and 35.0 parts by weight of metopine oil.
  • Raw material 55.0 parts by weight of salad oil, 45.0 parts by weight of metopine oil.
  • Raw materials 100 parts by weight of fresh pepper, 20 parts by weight of edible oil, 30 parts by weight of cane fruit oil, 60 parts by weight of vinegar, 12 parts by weight of salt, 15 parts by weight of garlic, 20 parts by weight of ginger, 10 parts by weight of chicken essence and allspice powder. 5 parts by weight.
  • Fresh peppers are selected and stalked, washed, crushed to a chili puree below 8mm, and set aside.
  • the obtained raw chili sauce was heated at 90 ° C for 30 to 60 minutes, and after cooling, the cane fruit oil was poured on the surface of the chili sauce, and the container lid was quickly tightened.
  • Raw materials 100 parts by weight of fresh pepper, 30 parts by weight of edible oil, 25 parts by weight of cane fruit oil, 30 parts by weight of vinegar, 15 parts by weight of salt, 20 parts by weight of garlic, 30 parts by weight of ginger, 10 parts by weight of chicken essence and allspice powder. 5 parts by weight.
  • Fresh peppers are selected and stalked, washed, crushed to a chili puree below 8mm, and set aside.
  • the obtained raw chili sauce was heated at 90 ° C for 30 to 60 minutes, and after cooling, the cane fruit oil was poured on the surface of the chili sauce, and the container lid was quickly tightened.
  • Raw materials 100 parts by weight of fresh pepper, 35 parts by weight of edible oil, 35 parts by weight of cane fruit oil, 25 parts by weight of vinegar, 20 parts by weight of salt, 30 parts by weight of garlic, 20 parts by weight of ginger, 15 parts by weight of chicken essence and allspice powder. 10 parts by weight.
  • Fresh peppers are selected and stalked, washed, crushed to a chili puree below 8mm, and set aside.
  • the obtained raw chili sauce was heated at 90 ° C for 30 to 60 minutes, and after cooling, the cane fruit oil was poured on the surface of the chili sauce, and the container lid was quickly tightened.
  • Raw materials 150 parts by weight of fresh peanuts, 30 parts by weight of metopine oil, 3 parts by weight of salt, and 7 parts by weight of white sugar.
  • the peeled cooked peanuts were added to a pulverizer for pulverization for 10 minutes to obtain a fine granule peanut powder.
  • Raw materials 120 parts by weight of fresh peanuts, 50 parts by weight of metopine oil, 5 parts by weight of salt, and 10 parts by weight of white sugar.
  • the peeled cooked peanuts were added to a pulverizer for pulverization for 10 minutes to obtain a fine granule peanut powder.
  • Raw material 200 parts by weight of fresh peanuts, 50 parts by weight of metopine oil, 15 parts by weight of salt, and 5 parts by weight of white sugar.
  • the peeled cooked peanuts were added to a pulverizer for pulverization for 10 minutes to obtain a fine granule peanut powder.
  • Raw material 70 parts by weight of sesame, 5 parts by weight of salt, 10 parts by weight of metoceroil, 2 parts by weight of white sugar, 11 parts by weight of water, and 2 parts by weight of chicken essence.
  • Material selection Select high-quality sesame seeds to remove inferior sesame seeds.
  • Material selection Select high-quality sesame seeds to remove inferior sesame seeds.
  • Raw materials 85 parts by weight of sesame, 10 parts by weight of salt, 15 parts by weight of metopine oil, 2 parts by weight of white sugar, 11 parts by weight of water, and 2 parts by weight of chicken essence.
  • Material selection Select high-quality sesame seeds to remove inferior sesame seeds.
  • Example 19 Modulates the function of human intestinal flora
  • the subjects were randomly divided into 19 groups of 20 persons each. Before the subjects were fed, the subjects' faeces were aseptically taken, and 16SrDNA sequencing was used to examine the intestinal flora as a reference for the background level.
  • group 1 took the control group
  • group 2 took the experimental group 1
  • group 3 took the experimental group 2
  • group 4 took the experimental group 3
  • group 5 took the experimental group 4
  • group 6 took the experimental group 5
  • group 7 Taking the experimental group 6, group 8 taking the experimental group 7, group 9 taking the experimental group 8, group 10 taking the experimental group 9, group 11 taking the experimental group 10, group 12 taking the experimental group 11, Group 13 taking experimental group 12, group 14 taking experimental group 13, group 15 taking experimental group 14, group 16 taking experimental group 15, group 17 taking experimental group 16, group 18 taking experimental group 17, group 19 taking experimental group 18, 4
  • the subjects' feces were taken and 16SrDNA sequencing was used to examine the intestinal flora, and compared with the background level, the multiple of the relative abundance was compared.
  • the fecal sample was first subjected to DNA extraction, and the V3 to V5 region of 16SrDNA was amplified by PCR, and then sequenced by the 454 platform, and the sequencing direction was V5->V3.
  • the raw data of each sample has an average of 7795 tags and a reading length of about 400bp.
  • the average number of tags used for analysis is 3235, and the read length is about 265 bp.
  • 16S analysis mainly uses mothur software (http://www.mothur.org/wiki/Mothur_manual) including quality control, OTU clustering, annotation and other analysis, and analyzes the relative abundance of each species based on the classification of data species. .
  • Test method 180 male Sprague-Dawley rats were used to prepare a small-dose streptozotocin-induced diabetic rat model according to the standard method. Three days later, the blood glucose levels of the rats were randomly divided into 18 groups, 10 in each group. Each group was administered by continuous intragastric administration for 8 days, once a day, and blood was taken two hours after the last administration, and the blood glucose level was measured.
  • Test subjects The SD rats were randomly divided into 18 groups, 10 in each group. The original SD rats were tested with the same diet control and activities. Group 1 took Minoberry oil applesauce and group 2 took beauty. Rattan oil kiwi sauce, group 3 taking Minoberry oil Sydney sauce, group 4 taking Mino fruit oil salad dressing A, group 5 taking Mino fruit oil salad dressing B, group 6 taking Mino fruit oil salad dressing C, group 7 Take the cane fruit oil and olive oil flavored blending oil, group 8 take the cane fruit oil and sesame oil flavor blending oil, group 9 take the cane fruit oil and salad oil flavor blending oil, group 10 take the cane fruit oil chili sauce A Group 11 taking Meitoguo oil chili sauce B, group 12 taking Meito fruit oil chili sauce C, group 13 taking Meito fruit oil peanut butter A, group 14 taking Meito fruit oil peanut butter B, group 15 taking Meito fruit oil Peanut butter C, group 16 taking Meito fruit oil sesame paste A, group 17 service Take the cane fruit oil sesame paste B and group
  • hyperlipidemia model rats Wistar male rats were randomly divided into 18 groups (10 in each group), namely hyperlipidemic rats (hyperlipidemia model control group), fed with high fat diet (high fat)
  • the feed formula is 93.8% for basic feed, 1.0% for cholesterol, 5.0% for lard, and 0.2% for bile salt. No test substance is given;
  • Rats with hyperlipidemia were randomly divided into 18 groups, 10 in each group. The rats with hyperlipidemia were tested with the original diet control and activities.
  • Group 1 took the cane fruit oil applesauce, group 2 taking vinegar oil kiwi sauce, group 3 taking meto fruit oil Sydney sauce, group 4 taking vine fruit oil salad dressing A, group 5 taking vine fruit oil salad dressing B, group 6 taking vine fruit oil salad dressing C, group 7 taking the cane fruit oil and olive oil flavor blending oil, group 8 taking the cane fruit oil and sesame oil flavor blending oil, group 9 taking the cane fruit oil and salad oil flavor blending oil, group 10 taking the cane fruit oil chili Sauce A, Group 11 Take Meito Fruit Oil chili Sauce B, Group 12 Take Meito Fruit Oil chili Sauce C, Group 13 Take Meito Fruit Oil Peanut Butter A, Group 14 Take Meito Fruit Oil Peanut Butter B, Group 15 Take Beauty Rattan oil peanut butter C, group 16 taking Meito fruit oil sesame paste A, group 17 taking Meito fruit oil sesame paste B, group 18 taking Mei
  • Example 22 human blood test experiment in lowering blood sugar and lowering blood fat
  • the subjects were randomly divided into 18 groups, 10 in each group. The subjects were treated with the same drug type, diet control and activity.
  • Group 1 took the cane fruit oil applesauce
  • group 2 took the cane fruit oil kiwi sauce.
  • Group 3 taking Minoberry oil Sydney sauce
  • Group 4 taking Minoberry oil salad dressing A
  • Group 5 taking Minoberry oil salad dressing B
  • Group 6 taking Minoberry oil salad dressing C
  • Group 7 took the cane fruit oil and olive oil flavored blending oil
  • group 8 took the cane fruit oil and sesame oil flavored blending oil
  • group 9 took the cane fruit oil and salad oil flavored blending oil
  • group 10 took the cane fruit oil chili sauce A
  • group 11 take Meito fruit oil chili sauce B
  • group 12 take Meito fruit oil chili sauce C
  • group 13 take Meito fruit oil peanut butter A
  • group 14 take Meito fruit oil peanut butter B
  • group 15 take Meito fruit Oil Peanut Butter C
  • Group 16 Take Mino Fruit Oil Sesame Sauce A
  • the cane fruit oil food flavoring agent of the invention has obvious health care functions of assisting blood sugar lowering and blood fat lowering, and has no harmful effect on the health of the tested population.
  • the food flavoring agent of the present invention is provided by Shenzhen Huada Gene Technology Co., Ltd., and is prepared according to Examples 1-18 of the present invention.
  • Subjects 180 patients with obesity who were over 20% overweight after physical examination, no other diseases, and normal visceral function.
  • the subjects were randomly divided into 18 groups, each group consisting of 10 people. According to the grouping of Table 1, Group 1 took Minoberry oil applesauce, Group 2 took Minoberry oil kiwi sauce, group 3 taking the cane fruit oil pear sauce, group 4 taking the cane fruit oil salad dressing A, group 5 taking the cane fruit oil salad dressing B, group 6 taking the cane fruit oil salad dressing C, group 7 taking the cane fruit oil and Olive oil flavored blending oil, group 8 taking vinegar oil and sesame oil flavored blending oil, group 9 taking cane fruit oil and salad oil flavor blending oil, group 10 taking meto fruit oil chili sauce A, group 11 taking metopine Oil chili sauce B, group 12 take Meito fruit oil chili sauce C, group 13 take Meito fruit oil peanut butter A, group 14 take Meito fruit oil peanut butter B, group 15 take Meito fruit oil peanut butter C, group 16 take beauty Rattan oil sesame paste A, group 17 taking the cane fruit oil sesame paste B, group 18 taking the cane fruit oil ses.
  • Overweight (%) (measured body weight - standard weight) / standard weight ⁇ 100%
  • the body weight, body fat percentage, body fat percentage, waist circumference and hip circumference decreased significantly after 30 days of continuous administration of the cane fruit oil food flavoring agent. It can be seen that the food flavoring agent of the invention has obvious weight loss effect on the human body, and no adverse reaction is observed during the taking.

Abstract

L'invention concerne l'utilisation d'huile sacha inchi dans la régulation de la flore du tractus intestinal d'animaux, l'utilisation d'huile sacha inchi dans la préparation d'aromatisants alimentaires et un procédé de régulation de la flore du tractus intestinal d'animaux et des aromatisants alimentaires. Il a été observé que l'huile sacha inchi est apte à augmenter de manière considérable le nombre de probiotiques dans le tractus intestinal d'animaux, ce qui permet de réguler considérablement la flore du tractus intestinal d'animaux et de permettre ensuite la prévention ou le traitement de l'obésité, du diabète et de l'hyperlipidémie par l'action de la flore du tractus intestinal.
PCT/CN2014/091922 2014-11-21 2014-11-21 Utilisation d'huile sacha inchi dans la régulation de la flore du tractus intestinal d'animaux WO2016078088A1 (fr)

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PCT/CN2014/091922 WO2016078088A1 (fr) 2014-11-21 2014-11-21 Utilisation d'huile sacha inchi dans la régulation de la flore du tractus intestinal d'animaux
CN201480083554.0A CN106998723A (zh) 2014-11-21 2014-11-21 美藤果油在调节动物肠道菌群中的用途

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PCT/CN2014/091922 WO2016078088A1 (fr) 2014-11-21 2014-11-21 Utilisation d'huile sacha inchi dans la régulation de la flore du tractus intestinal d'animaux

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CN109364110B (zh) * 2018-12-14 2022-02-22 盐城工学院 一种从美藤果壳提取黄酮的方法及其应用
CN112494531A (zh) * 2020-10-15 2021-03-16 华南农业大学 一种美藤果叶提取物及其提取方法和应用

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