Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
  • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• • A—HUMAN NECESSITIES
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  • A61K39/12—Viral antigens
  • A61K39/215—Coronaviridae, e.g. avian infectious bronchitis virus
  • A61K39/225—Porcine transmissible gastroenteritis virus
• • A—HUMAN NECESSITIES
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  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
  • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
  • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
  • A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame
• • A—HUMAN NECESSITIES
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  • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
  • A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
• • A—HUMAN NECESSITIES
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  • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
  • A61K47/32—Macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds, e.g. carbomers, poly(meth)acrylates, or polyvinyl pyrrolidone
• • A—HUMAN NECESSITIES
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  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0012—Galenical forms characterised by the site of application
  • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
• • A—HUMAN NECESSITIES
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  • A61P31/12—Antivirals
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  • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
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  • A61P31/12—Antivirals
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• • A—HUMAN NECESSITIES
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  • A61P37/02—Immunomodulators
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• • A—HUMAN NECESSITIES
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  • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
  • A61K2039/525—Virus
  • A61K2039/5254—Virus avirulent or attenuated
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  • A61K2039/55—Medicinal preparations containing antigens or antibodies characterised by the host/recipient, e.g. newborn with maternal antibodies
  • A61K2039/552—Veterinary vaccine
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  • A61K2039/70—Multivalent vaccine
• • C—CHEMISTRY; METALLURGY
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  • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
  • C12N2710/00011—Details
  • C12N2710/16011—Herpesviridae
  • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
  • C12N2710/16734—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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  • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
  • C12N2720/00011—Details
  • C12N2720/12011—Reoviridae
  • C12N2720/12311—Rotavirus, e.g. rotavirus A
  • C12N2720/12334—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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  • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
  • C12N2750/00011—Details
  • C12N2750/14011—Parvoviridae
  • C12N2750/14034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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  • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
  • C12N2760/00011—Details
  • C12N2760/16011—Orthomyxoviridae
  • C12N2760/16034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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  • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
  • C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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  • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
  • C12N2770/00011—Details
  • C12N2770/10011—Arteriviridae
  • C12N2770/10034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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  • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
  • C12N2770/00011—Details
  • C12N2770/20011—Coronaviridae
  • C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Definitions

• the present invention pertains to liquid stable porcine vaccines that comprise a live porcine virus.
• the invention also pertains to the manufacture of such vaccines and methods of vaccinating animal subjects.
• viruses that can infect swine.
• viruses include porcine reproductive and respiratory syndrome virus (PRRS), transmissible gastroenteritis virus (TGE), porcine pseudorabies virus (PPRV), porcine parvovirus (PPV), swine influenza virus (SIV), porcine rotavirus (PRV) and porcine epidemic diarrhea virus (PED).
• PRRS porcine reproductive and respiratory syndrome virus
• TGE transmissible gastroenteritis virus
• PPRV porcine pseudorabies virus
• PDV porcine parvovirus
• SIV swine influenza virus
• PRV porcine rotavirus
• PED porcine epidemic diarrhea virus
• bacteria that can infect swine too, including Pasteur ella multocida of multiple serotypes, Salmonella ssp., Escherichia coli of multiple pillus types, Haemophilus parasuis, Lawsonia intracellularis, Mycoplasma ssp., Bordetella bronchiseptica, Erysipelas ssp., Campylobacter ssp., Actinobacillus pleuropneumoniae, Clostridium perfringens and Clostridium difficile.
• multivalent live attenuated viral or bacterial vaccines can be safely administered that limit the number of vaccine injections required. Accordingly, there are many commercially available multivalent live virus vaccines that protect against multiple pathogens.
• live attenuated swine viruses have been unstable when stored in liquid solutions. Therefore, most live attenuated swine virus vaccines are lyophilized, i.e., freeze-dried or frozen, prior to their long-term storage.
• the live attenuated porcine virus is commonly mixed as a suspension in water with a protective agent, frozen, and then dehydrated by sublimation and secondary drying during the
• lyophilized swine vaccines that comprise live attenuated viruses are manufactured with augmented titers.
• increased titers can lead to significant adverse events should the lyophilization process actually lead to less loss of activity than anticipated. This is particularly problematic for the swine farmer because, at minimum, such an adverse event often leads to lower daily weight gain for the pigs, which translates to lower profits at sale. Therefore, great care is required to formulate a vaccine to contain a virus titer that is not only safely below the amount that leads to adverse events, but that also maintains sufficient efficacy in view of the virus titer loss due to lyophilization and subsequent storage.
• the use of a liquid stable vaccine would not need to be restricted by being placed into small glass containers, but rather the vaccine could be stored in plastic bags that could have a large range in sizes. Moreover, following the administration of the vaccine to the swine, the plastic bags can easily be destroyed by burning in a small contained fire.
• the present invention provides novel liquid stable, live, porcine virus vaccines, as well as their corresponding immunogenic compositions.
• the liquid stable, live, porcine virus vaccines of the present invention can remain efficacious for extended periods such as 6, 7, 9 months or longer (e.g., about 1 to up to 3 years).
• the present invention also provides methods of administering such vaccines to a pig.
• the present invention further provides methods of preventing a disease in an animal, e.g., a pig, through administering a vaccine of the present invention.
• the present invention provides liquid stable vaccines, including multivalent vaccines that comprise a live virus.
• the live virus is an attenuated virus.
• the live virus is a recombinant virus.
• the live virus is both attenuated and recombinant.
• Recombinant viruses of the present invention can also encode a heterogeneous protein.
• the heterogeneous protein is a virus, parasite or bacterial antigen.
• the vaccine comprises a sugar additive that is a sugar alcohol and/or an amino acid.
• the vaccine comprises 5 to 40% (w/v) of a sugar alcohol.
• the vaccine comprises 10 to 30% (w/v) of a sugar alcohol.
• the vaccine comprises 15 to 25% (w/v) of a sugar alcohol.
• the vaccine comprises 10 to 20% (w/v) of a sugar alcohol.
• the vaccine comprises 20 to 25% (w/v) of a sugar alcohol.
• the vaccine comprises 25 to 40% (w/v) of a sugar alcohol.
• the vaccine comprises 12 to 18% (w/v) of a sugar alcohol. In even more particular embodiments, the vaccine comprises about 15% (w/v) of a sugar alcohol. In related embodiments, the vaccine comprises about 23% (w/v) of a sugar alcohol.
• the liquid stable virus vaccines of the present invention comprise two or more sugar alcohols, with the total amount of the sugar alcohol in the liquid stable vaccines being 5- 40% (w/v). In other such embodiments, the liquid stable virus vaccines of the present invention comprise two or more sugar alcohols, with the total amount of the sugar alcohol in the liquid stable vaccines being 25-40% (w/v).
• the sugar alcohol is sorbitol.
• the sugar additive is mannitol.
• the liquid stable vaccines further comprise a sugar additive that is a non- sugar alcohol, wherein the total amount of the sugar alcohol and the non-sugar alcohol in the liquid stable vaccine is 15-40% (w/v).
• the liquid stable vaccines further comprise a sugar additive that is a non-sugar alcohol in which the total amount of the sugar alcohol and the non-sugar alcohol in the liquid stable vaccine is 25-40% (w/v).
• the non-sugar alcohol, sugar additive is trehalose.
• the non-sugar alcohol, sugar additive is dextrose. In yet other embodiments, the non-sugar alcohol, sugar additive is sucrose. In particular embodiments of this type, the sugar additive is a combination of sucrose (non-sugar alcohol) and sorbitol (sugar alcohol). In more particular embodiments of this type, the sugar additive is a combination of 10-25% sorbitol and 5-20% sucrose. In other embodiments of this type, the sugar additive is a combination of 15-30% sorbitol and 10-25% sucrose. In still more particular embodiments of this type, the sugar additive is a combination of 15% sorbitol and 10% sucrose. In particular embodiments the non- sugar alcohol, sugar additive is actually a combination of two or more non-sugar alcohol, sugar additives.
• the liquid stable vaccines of the present invention can range in pH from pH 6.0 to pH 8.0.
• the pH range is from pH 6.5 to pH 7.8.
• the pH range is from pH 6.8 to pH 7.5.
• the pH range is from pH 6.6 to pH 7.4.
• the pH range is from pH 7.0 to pH 7.4.
• the pH is 7.2.
• the liquid stable vaccines of the present invention can comprise a buffer.
• the buffer comprises 2.5 to 50 mM phosphate, e.g., sodium phosphate ( aPHOS) or potassium phosphate (KPHOS).
• the buffer comprises 5 to 25 mM phosphate.
• the buffer comprises 10 to 20 mM phosphate.
• the buffer (i.e., buffer solution) can further comprise 0.15 to 0.75 M arginine.
• the buffer comprises 2.5 to 50 mM phosphate and 0.15 to 0.75 M arginine.
• the buffer comprises 5 to 25 mM phosphate and 0.15 to 0.75 M arginine.
• the buffer comprises 10 to 20 mM phosphate and 0.3 to 0.5 M arginine.
• the buffer comprises 2.5 to 50 mM phosphate.
• the buffer comprises 5 to 25 mM Tris.
• the buffer comprises 10 to 20 mM Tris.
• the Tris buffer comprises histidine.
• the liquid stable vaccines of the present invention can comprise an amino acid.
• the amino acid is arginine.
• the amino acid is methionine.
• the amino acid is glycine.
• the amino acid is glutamic acid.
• the liquid stable vaccines comprise both arginine and methionine.
• the liquid stable vaccines comprise both arginine and glycine.
• the liquid stable vaccines comprise both glycine and methionine.
• the liquid stable vaccines comprise both glutamic acid and methionine.
• the liquid stable vaccines comprise both glutamic acid and glycine.
• the liquid stable vaccines comprise both glutamic acid and arginine.
• the liquid stable vaccines comprise arginine, glutamic acid, and methionine. In other embodiments, the liquid stable vaccines comprise arginine, glutamic acid, and glycine. In yet other embodiments, the liquid stable vaccines comprise arginine, glutamic acid, and methionine. In still other embodiments, the liquid stable vaccines comprise arginine, glycine, and methionine. In yet other embodiments, the liquid stable vaccines comprise arginine, glycine, and methionine. In particular embodiments, the liquid stable vaccines comprise arginine, glycine, methionine, and glutamic acid.
• the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is 0.15 to 0.75 M. In related embodiments, the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is 0.25 to 0.75 M. In more particular embodiments, the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is 0.2 to 0.6 M. In more particular embodiments, the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is 0.2 to 0.5 M. In still other embodiments, the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is 0.25 to 0.45 M.
• the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is about 0.45 M. In other particular embodiments, the final concentration of arginine, or glutamic acid, or glycine in the liquid stable vaccine is about 0.3 M.
• the final combined concentration of arginine together with glutamic acid and/or glycine in the liquid stable vaccine is 0.15 to 0.75 M. In related embodiments, the final concentration of arginine together with glutamic acid and/or glycine in the liquid stable vaccine is 0.25 to 0.75 M. In other embodiments, the final combined concentration of arginine together with glutamic acid and/or glycine in the liquid stable vaccine is 0.2 to 0.6 M. In more particular embodiments, the final combined concentration of arginine together with glutamic acid and/or glycine in the liquid stable vaccine is 0.3 to 0.5 M.
• the final concentration of arginine and glutamic acid, or glycine in the liquid stable vaccine is 0.25 to 0.45 M. In even more particular embodiments, the final combined concentration of arginine together with glutamic acid and/or glycine in the liquid stable vaccine is about 0.45 M. In other particular embodiments, the final concentration of arginine together with glutamic acid and/or glycine in the liquid stable vaccine is about 0.3 M.
• the final concentration of methionine in the liquid stable vaccine is 0.025 to 0.3 M. In related embodiments, the final concentration of methionine in the liquid stable vaccine is 0.04 to 0.15 M. In more particular embodiments, the final concentration of methionine in the liquid stable vaccine is 0.06 to 0.09 M. In even more particular embodiments, the final concentration of methionine in the liquid stable vaccine is about 0.07 M.
• the liquid stable vaccines of the present invention also can comprise a stabilizer protein.
• the stabilizer protein can be an intact protein and/or a protein hydrolysate.
• the stabilizer protein is gelatin. In more particular embodiments the stabilizer protein contained by the liquid stable vaccine of the present invention is 0.4 to 1.6% gelatin. In alternative embodiments the stabilizer protein is a hydrolysate of whole casein. In particular embodiments of this type the stabilizer protein contained by the liquid stable vaccine of the present invention is 0.5-2.0% of a hydrolysate of whole casein. In certain embodiments the hydrolysate of whole casein is a proteolytic hydrolysate of whole casein. In yet other embodiments, the stabilizer protein contained by the liquid stable vaccine of the present invention is lactoglobulin or a lactalbumin hydrolysate.
• liquid stable vaccines of the present invention can also further comprise a chelating agent.
• chelating agents can include, but are not limited to:
• EDTA ethylenediaminetetraacetic acid
• BAPTA l,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
• EGTA ethylene glycol tetraacetic acid
• DMSA dimercaptosuccinic acid
• DTP A diethylene triamine pentaacetic acid
• DMPS 2,3-Dimercapto-l -propanesulfonic acid
• concentration of such chelating agents in the liquid vaccines of the present invention can vary from about 50 ⁇ to 10 mM.
• the chelating agent is ethylenediaminetetraacetic acid (EDTA).
• EDTA ethylenediaminetetraacetic acid
• the liquid stable vaccine comprises 0.050 to 1 mM EDTA.
• the liquid stable vaccine comprises 0.25 to 0.75 mM EDTA.
• the liquid stable vaccine comprises about 0.5 mM EDTA.
• the liquid stable vaccines of the present invention can further comprise one or more free radical scavengers and/or antioxidants as a component.
• a vaccine of the present invention comprises ascorbic acid.
• the liquid stable vaccine comprises about 0.5 mM ascorbic acid.
• the vaccine comprises ⁇ ⁇ -tocopherol.
• the liquid stable vaccine comprises about 0.5 mM ⁇ ⁇ -tocopherol.
• the vaccine comprises glutathione.
• the liquid stable vaccine comprises about 3 mM glutathione.
• the vaccine comprises both ⁇ ⁇ -tocopherol and ascorbic acid.
• the vaccine comprises both ⁇ -tocopherol and glutathione. In still another embodiment, the vaccine comprises both glutathione and ascorbic acid. In yet another embodiment the vaccine comprises ascorbic acid, / ⁇ -tocopherol, and glutathione.
• the liquid stable vaccines of the present invention are maintained in sealed containers.
• the liquid stable vaccines of the present invention are maintained in sealed containers that have an inert gas such as argon, nitrogen, or helium, above the liquid (e.g., have been back-filled with the inert gas).
• the liquid stable vaccines of the present invention can further comprise an adjuvant.
• the adjuvant is aluminum phosphate.
• the adjuvant is aluminum hydroxide.
• the adjuvant is a low molecular weight copolymer adjuvant which can form cross-linkage in solution to become a high molecular weight gel.
• the adjuvant is made up of gel particles of sodium acrylate in water.
• the adjuvant is a combination of two or more such adjuvants.
• the liquid stable vaccines of the present invention can further comprise a detergent and/or surfactant.
• the surfactant is a polyoxyethylene-polyoxypropylene block copolymer.
• the liquid stable vaccine comprises about 0.01% polyoxyethylene-polyoxypropylene block copolymer.
• the polyoxyethylene-polyoxypropylene block copolymer is PLURONIC ® F-68.
• the liquid stable vaccines of the present invention can comprise one or more live attenuated swine virus.
• the live attenuated swine virus is transmissible
• the live attenuated swine virus is porcine reproductive and respiratory syndrome virus (PRRS).
• PRRS porcine epidemic diarrhea virus
• the live attenuated swine virus is porcine epidemic diarrhea virus (PED).
• the live attenuated swine virus is swine influenza virus (SIV).
• the live attenuated swine virus is porcine rotavirus (PRV).
• PRV porcine parvo virus
• the live attenuated swine virus is porcine pseudorabies virus (PPRV).
• the present invention further provides any combination of two or more of these live attenuated swine viruses.
• the present invention provides liquid stable vaccines that are multivalent vaccines.
• the multivalent vaccines of the present invention can contain any combination of porcine viruses.
• the multivalent vaccines of the present invention comprise both killed porcine viruses and live attenuated porcine viruses.
• the multivalent vaccine comprises a live porcine reproductive and respiratory syndrome virus (PRRS) together with a live attenuated porcine epidemic diarrhea virus (PED) and/or a killed porcine circovirus antigen (PCV) and/or a recombinant subunit of PCV.
• PRRS live porcine reproductive and respiratory syndrome virus
• PED live attenuated porcine epidemic diarrhea virus
• PCV killed porcine circovirus antigen
• the multivalent vaccine comprises killed SrV, killed and/or subunit porcine circovirus (PCV), together with live attenuated transmissible gastroenteritis virus (TGE) and live attenuated porcine parvovirus (PPV).
• the multivalent vaccine comprises killed porcine circovirus antigen (PCV) and/or recombinant subunit thereof, killed swine influenza virus of multiple serotypes, live and/or killed attenuated transmissible gastroenteritis virus (TGE), and live attenuated porcine rotavirus (PRV).
• the liquid stable vaccines of the present invention can further comprise a killed virus and/or a killed bacterium (e.g., a bacterin) and/or a sub-fraction of a bacterin.
• a killed virus and/or a killed bacterium e.g., a bacterin
• any of the liquid stable vaccines of the present invention that comprise one or more live virus vaccines can further comprise a killed virus and/or killed bacterium and/or a sub-fraction of a bacterin.
• these vaccines can further comprise adjuvants e.g., as indicated herein.
• the multivalent vaccine comprises one or more Clostridium perfringens inactivated toxoid, pilus antigen extracted from E. coli bacteria e.g.
• the multivalent vaccine comprises a live porcine reproductive and respiratory syndrome virus (PRRS) and a killed Mycoplasma hyopneumoniae (M. hyo), and/or an inactivated Lawsonia intracellularis bacterin.
• the multivalent vaccine comprises a killed porcine circovirus antigen (PCV) and/or a recombinant subunit of PCV, a killed Mycoplasma hyopneumoniae (M. hyo), an inactivated Lawsonia intracellularis bacterin, together with a live porcine reproductive and respiratory syndrome virus (PRRS).
• the present invention further provides methods of aiding in the protection of swine against a clinical disease that arises from a swine virus infection comprising administering a vaccine of the present invention to the animal.
• the present invention provides methods that comprise administering to a porcine any of the liquid stable vaccines of the present invention.
• the administration is performed mucosally (by intranasal or oral route).
• the administration is performed parenterally.
• the administration is performed intradermally.
• the administration is performed transdermally.
• a vaccine of the present invention is administered to the pig intramuscularly.
• the present invention also includes the use of primary and/or booster vaccines.
• the method comprises administering to the pig a liquid stable vaccine of the present invention that comprises one or more live virus, e.g., a live attenuated virus.
• the liquid stable vaccine comprises live attenuated PRRS, live attenuated TGE, a live PRV, live attenuated PED and/or live attenuated PPRV.
• the liquid stable vaccine comprises live attenuated PRRS, two serotypes of PRV, live attenuated TGE, and live attenuated PPRV as an expression vector for other vaccine antigens.
• liquid stable vaccines of the present invention one or more attenuated or killed bacterial antigens such as P. multocida, Salmonella ssp., an is. coli including pilus types K99, K88, 987P, P41,
• A. pleuropneumoniae, B. bronchiseptica, L. intracellularis, M. hyopneumoniae, C. perfringens toxoids from types A, C, and/or D can also be administered.
• swine vaccines both monovalent and multivalent
• Tables 1A-1C below.
• the method comprises combining a therapeutically effective amount of a live attenuated virus with a e.g., 5-40% (w/v) or 15-40% (w/v) sugar additive, (e.g., a sugar alcohol or a combination of a sugar alcohol with a non-sugar alcohol), an amino acid, and a buffered solution at pH 6.0 to pH 8.0 to form a liquid stable vaccine.
• a sugar additive e.g., a sugar alcohol or a combination of a sugar alcohol with a non-sugar alcohol
• an amino acid e.g., a buffered solution at pH 6.0 to pH 8.0 to form a liquid stable vaccine.
• the amino acid can be arginine, glycine, glutamic acid, methionine, or combinations of arginine, glycine, glutamic acid and/or methionine.
• the arginine and/or glycine and/or glutamic acid has a final concentration of 0.15 to 0.75 M in the liquid stable vaccine.
• the vaccine further comprises methionine at a final concentration of 0.025 to 0.3 M in the liquid stable vaccine.
• the therapeutically effective amount of a live attenuated virus is a therapeutically effective amount of a live attenuated swine virus.
• the therapeutically effective amount of a live attenuated swine virus includes therapeutically effective amounts of live attenuated TGE virus, live attenuated PRRS virus, live attenuated PRV, live attenuated PED virus, live attenuated PPRV and a live attenuated SIV virus, or a live attenuated virus expressing SIV proteins.
• liquid stable swine virus vaccines of the present invention comprise live viruses, e.g., live attenuated viruses
• live attenuated viruses heretofore particular care would have been needed during the formulation of the vaccine to maintain the titer of the attenuated viruses at a level that is safely below that which can lead to a significant adverse event.
• live attenuated swine virus vaccines are lyophilized, and lyophilization can lead to substantial declines in the efficacy of the attenuated live virus vaccines both due to the lyophilization process itself, as well as over time during long-term storage.
• the present invention has overcome this problem by providing liquid stable swine vaccines that remain efficacious, even during storage, without needing to increase the initial titer of the live attenuated viral antigen above a reliably safe level.
• the present invention provides a means for lowering the cost of manufacture of the vaccines provided by significantly reducing the amount of live attenuated swine viruses necessary to make such a safe and efficacious vaccine.
• the live attenuated swine virus vaccines of the present invention are more convenient to use than their lyophilized counterparts.
• the present invention provides safe and efficacious live attenuated swine virus vaccines that can be stored as liquids at refrigerated temperatures and still remain stable for 5 to 7 months, 6 to 9 months, 9-12 months, 12 to 18 months, 18 to 24 months and/or even longer.
• the liquid stable vaccines of the present invention do not have to be used as soon as it is rehydrated, because they are always hydrated. Since swine vaccines often come in 100 dose vials, as the largest presentation, those with larger farms will use hundreds of glass vials and discard the hazardous waste leftover from these vaccines in the trash.
• larger farms can buy larger packages (e.g., made of plastic) of vaccine, which they can use over weeks or months, providing the vaccine is handled properly and not contaminated, and then burn the residual plastic container when the vaccine is used up, thus decontaminating the container. This opens a whole new exclusive market of convenience to larger farms.
• larger packages e.g., made of plastic
• the liquid stable live swine virus vaccines of the present invention can include swine viruses of any type.
• the liquid stable live virus vaccines of the present invention can include both enveloped and non-enveloped swine viruses.
• the liquid stable live virus vaccines of the present invention can include live attenuated swine viruses having single-stranded RNA genomes, single-stranded DNA genomes, or double-stranded DNA genomes.
• a "vaccine” is a composition that is suitable for application to an animal (including, in certain embodiments, humans) which upon administration to the animal induces an immune response strong enough to minimally aid in the protection from a clinical disease arising from an infection with a wild-type micro-organism, i.e., strong enough for aiding in the prevention of the clinical disease, and/or preventing, ameliorating, or curing the clinical disease.
• a vaccine includes multivalent vaccines.
• a "multivalent vaccine” is a vaccine that comprises two or more different antigens.
• the multivalent vaccine stimulates the immune system of the recipient against two or more different pathogens.
• a "liquid stable” vaccine is a vaccine maintained as a liquid (including a liquid multivalent vaccine) that remains efficacious for at least six months when stored at or below 7°C (e.g., in a standard refrigerator, and/or at 0°C - 7°C).
• a liquid stable vaccine remains efficacious when stored at or below 7°C for at least 6 months.
• a liquid stable vaccine remains efficacious when stored at or below 7°C for at least 9 months.
• a liquid stable vaccine remains efficacious when stored at or below 7°C for at least 1 year.
• a liquid stable vaccine remains efficacious when stored at or below 7°C for at least 1.5 years.
• a liquid stable vaccine remains efficacious when stored at or below 7°C for at least 2.0 to 3 years.
• the terms “protect”, “protecting”, “provide protection to”, “providing protection to”, and “aids in the protection” do not require complete protection from any indication of infection.
• “aids in the protection” can mean that the protection is sufficient such that, after challenge, symptoms of the underlying infection are at least reduced, and/or that one or more of the underlying cellular, physiological, or biochemical causes or mechanisms causing the symptoms are reduced and/or eliminated.
• “reduced,” as used in this context, means relative to the state of the infection, including the molecular state of the infection, not just the physiological state of the infection.
• prophylactically-effective amount refers to the amount of a composition that when administered to swine significantly reduces the likelihood and/or extent of an
• Methodaphylaxis is the timely mass medication of an entire group of animals to eliminate or minimize an expected outbreak of disease, e.g. in one or more animals at high risk of
• chemoprophylaxis refers to the administration of a medication/treatment, e.g., one or more prophylactic compositions, for the purpose of preventing or reducing viral, bacterial, and/or parasitic infection/infestation; and/or preventing or reducing disease and/or symptoms related to that infection/infestation.
• prophylactic composition refers to any agent used singularly or in combination with other agents that significantly reduces the likelihood and/or extent of an infection/infestation due to a given pathogen in swine.
• the swine are at high risk of developing swine enteric disease following commingling, changes in weather, changes in nutrition, and/or other stressors that can initiate a symptom and/or a disease related to the presence of the viral, bacterial, or parasitic pathogens commonly associated with swine, targeted by the agent or combination of agents.
• the term "therapeutically effective amount” is an amount of a given antigen, e.g., a live attenuated swine virus, which is sufficient to provide protection to and/or aid in the protection from the pathogen that the antigen is being administered to protect against, when provided in a single administration and/or when intended, provided as an initial administration with one or more subsequent booster administration(s).
• an "efficacious" vaccine comprises a therapeutically effective amount of a given antigen.
• An “efficacious” vaccine retains sufficient titer for a given antigen to be compliant with the regulatory requirements for that antigen for the jurisdiction where the vaccine is
• the term "pharmaceutically acceptable” is used adjectivally to mean that the modified noun is appropriate for use in a pharmaceutical product.
• an excipient in a pharmaceutical vaccine it characterizes the excipient as being compatible with the other ingredients of the composition and not disadvantageously deleterious to the intended recipient.
• carrier refers to a diluent, adjuvant, excipient, or vehicle with which the compound is administered.
• Pharmaceutical acceptable carriers can be sterile liquids, such as water and/or oils, including those of petroleum, animal, vegetable or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like.
• Water or aqueous solution saline solutions and aqueous sugar, e.g., dextrose and/or glycerol, solutions can be employed as carriers, particularly for injectable solutions.
• the carrier can be and/or comprise a hydrocolloid and/or polymer solution e.g., to thicken the porcine vaccines that are to be sprayed on to the pigs.
• an "adjuvant” is a substance that is able to favor or amplify the cascade of immunological events, ultimately leading to a better immunological response, i.e., the integrated bodily response to an antigen.
• An adjuvant is in general not required for the immunological response to occur, but favors or amplifies this response.
• systemic administration is administration into the circulatory system of the body (comprising the cardiovascular and lymphatic system), thus affecting the body as a whole rather than a specific locus such as the gastro-intestinal tract (via e.g., oral administration) and the respiratory system (via e.g., intranasal administration).
• Systemic administration can be performed e.g., by administering into muscle tissue (intramuscular), into the dermis (intradermal, transdermal, or supradermal), underneath the skin (subcutaneous), underneath the mucosa (submucosal), in the veins (intravenous) etc.
• a "sugar additive” is a 5 to 12 carbon sugar (e.g., sucrose, maltose, trehalose, dextrose, lactose, glucose, fructose, galactose) or sugar alcohol/polyol (e.g., sorbitol, mannitol, arabitol, inositol, maltitol).
• sugar alcohol/polyol e.g., sorbitol, mannitol, arabitol, inositol, maltitol.
• the percent (%) of the sugar additive is provided as a weight (w) of the sugar additive to the volume (v) of the vaccine, (w/v) in the vaccine.
• non-reducing sugar is a sugar additive which in basic aqueous medium does not generate any compounds containing an aldehyde group.
• non-reducing sugars of the present invention include sucrose and trehalose.
• non-sugar alcohol and “non-alcohol sugar” are used interchangeably.
• a sugar additive that is a “non-sugar alcohol” (or “non-alcohol sugar”) as used herein, can be any sugar additive that is not a sugar alcohol, e.g., a non-reducing sugar.
• the percent (%) of a solid additive, e.g., sugar additive or gelatin, in a vaccine is based on a 1% solution being lg of solid/100 ml of vaccine volume (w/v).
• the percent (%) of a liquid additive, e.g., ethanol, in a vaccine is based on a 1% solution being 1 ml of liquid additive /100 ml of vaccine volume (v/v).
• the term, "approximately,” is used interchangeably with the term “about” and generally signifies that a value is within twenty-five percent of the indicated value, unless otherwise indicated, i.e., a concentration of "about” 2 mM EDTA can be 1.5 mM to 2.5 mM EDTA.
• the pH value provided is the pH value determined/measured at 25°C.
• the liquid stable vaccines of the present invention can comprise a buffer.
• Buffers for use in the liquid stable vaccines of the present invention include but are not limited to: potassium phosphate, sodium phosphate, Tris, Tris-Histidine, BIS-Tris, BIS-Tris-Propane, sodium or potassium pyrophosphate, imidazole, PIPES, ACES, MOPS, MOPSO, BES, TES, tricine, glycylglycine, and HEPES.
• the buffers can be brought to the desired pH with the use of any suitable counter ion.
• the hydro lysate of whole casein that can be used in the liquid stable vaccines of the present invention can be obtained by a number of procedures including e.g., as an acid hydro lysate or an enzymatic hydro lysate.
• Such hydro lysates contain in the form of mixed amino acids and peptides having all of the amino acids originally present in casein.
• One pancreatic hydro lysate of whole casein that can be used in the liquid stable vaccines of the present invention is sold as CASEIN HYDROLYSATE ENZYMATIC ® by MP Biomedicals. Comparable products are sold under the name of NZ-AMINE ® , NZ-AMINE ® A, NZ-AMINE ® AS, and NZ-AMINE ® B, and Tryptone by Sigma-Aldrich.
• a liquid stable multivalent swine vaccine of the present invention can include two or more antigens including one or more of the following live attenuated swine viruses: PRRS, PEDV, PRV, TGEV, PPRV, SIV, and/or a recombinant SIV encoding one or more heterologous antigens.
• a liquid stable multivalent swine vaccine of the present invention can also include one or more of the following live attenuated viruses: PRRS, PEDV, PRV, TGEV, PPRV, SIV, and/or a recombinant SIV encoding one or more heterologous antigens, along with one or more killed swine viruses.
• a liquid stable vaccine of the present invention can be subsequently combined with one or more live attenuated or killed bacterial vaccine comprising an antigen such as Pasteurella multocida, Salmonella ssp., Escherichia coli of multiple pillus types, Actinobacillus
• Escherichia coli multiple pilus types including
• the vaccines of the present invention can either contain an adjuvant or alternatively not contain an adjuvant, often depending on the antigen(s) that the vaccine contains.
• the adjuvant comprises an aluminum salt.
• the aluminum salt is chosen from the group consisting of aluminum phosphate, aluminum potassium phosphate, and aluminum hydroxide.
• One aluminum phosphate adjuvant is REHYDROPHOS ® (General Chemical, Parsippany, New Jersey).
• aluminum hydroxide adjuvants include: REHYDROGEL ® , REHYDROGEL ® HP A, or REHYDROGEL ® LV (General
• adjuvants include hydrocarbon oils, polymers, saponins and/or an adjuvant made up of gel particles of sodium acrylate in water, e.g., MONTANIDETM PET GEL ATM (Seppic, Paris France).
• One low molecular weight copolymer adjuvant can form cross-linkage in solution to become a high molecular weight gel, e.g., POLY GENTM (MVP Laboratories, Omaha).
• the amount of adjuvant is usually between about 1% and 20% (v/v) in the vaccine. In particular embodiments the amount of adjuvant is between about 2% to 10% (v/v). In more particular embodiments the amount of adjuvant is between about 3% to 6% (v/v).
• liquid stable virus vaccines of the present invention may be administered by any conventional means, for example, by systemic administration, including by parenteral administration such as, without limitation, subcutaneous or intramuscular
• liquid stable virus vaccines of the present invention also may be any liquid stable virus vaccines of the present invention.
• liquid stable virus vaccines of the present invention also may be any liquid stable virus vaccines of the present invention.
• the vaccines may be administered by mucosal administration, such as by intranasal, oral, and/or ocular administration.
• the vaccines may be administered via a skin patch, in a delayed release implant, scarification, or topical administration.
• a liquid stable virus vaccine of the present invention also may be administered via the drinking water and/or food of the recipient swine.
• the vaccines (including multivalent vaccines) of the present invention also may be administered as part of a combination therapy, i.e., a therapy that includes, in addition to the vaccine itself, administering one or more additional active agents, therapies, etc.
• the amount of vaccine that constitutes a "therapeutically effective” amount may be more or less than the amount of vaccine that would constitute a “therapeutically effective” amount if the vaccine were to be administered alone.
• Other therapies may include those known in the art, such as, e.g., analgesics, fever-reducing medications, expectorants, anti-inflammation medications, antihistamines, and/or administration of fluids.
• the immunogenicity level may be determined experimentally by vaccine dose titration and challenge study techniques generally known in the art. Such techniques typically include vaccinating a number of animal subjects with the vaccine at different dosages and then challenging the animal subjects with the virulent virus to determine the minimum protective dose.
• Factors affecting the preferred dosage regimen may include, for example, the breed (e.g., of a swine), age, weight, diet, activity, lung size, and condition of the subject; the route of administration; the efficacy, safety, and duration-of-immunity profiles of the particular vaccine used; whether a delivery system is used; and whether the vaccine is administered as part of a drug and/or vaccine combination.
• the dosage actually employed can vary for specific animals, and, therefore, can deviate from the typical dosages set forth above. Determining such dosage adjustments is generally within the skill of those in the art of vaccine development using conventional means.
• the volume with which such a dose can be administered typically lies between 0.1 mL (intradermal applications) and 2.0 mL.
• a typical range for the administration volume is between 0.2 and 1.0 mL, and about 0.2 to 0.5 mL for intradermal administration.
• the vaccine may be administered to the vaccine recipient at a single time or alternatively, two or more times over days, weeks, months, or years. In some embodiments, the vaccine is administered at least two times. In certain such embodiments, for example, the vaccine is administered twice, with the second dose (e.g., a booster) being administered at least 2 weeks after the first dose. In particular embodiments, the vaccine is administered twice, with the second dose being administered no longer than 8 weeks after the first dose. In other
• the first and subsequent dosages may vary, such as in amount and/or form. Often, however, the dosages are the same in amount and form. When only a single dose is administered, the amount of vaccine in that dose alone generally comprises a therapeutically effective amount of the vaccine. When, however, more than one dose is administered, the amounts of vaccine in those doses together may constitute a therapeutically effective amount.
• a vaccine may be initially administered, and then a booster may be administered from 2 to 12 weeks later, as discussed above. However, subsequent administrations of the vaccine may be made on an annual (1 -year) or bi-annual (2 -year) basis, regardless as to whether a booster was administered or not.
• amphotericin B 10-100 ⁇ g/mL tetracycline, 10-100 units/mL nystatin (mycostatin), 10-100 units/mL penicillin, 10-100 ⁇ g streptomycin, 10-100 ⁇ g polymyxin B, and 10-100 ⁇ g neomycin.
• US Pharmacopeial (USP) or higher grade sucrose and sorbitol are purchased from Fisher Scientific. Molecular biology grade L-arginine monohydrochloride with purity higher than 98% are purchased from Sigma. NZ Amine (bloom 250) solutions are prepared from the best available commercial reagents.
• Stabilizer solution and vaccine blend pH adjustment The pH of the final vaccine blend can be critical to the stability of the virus in liquid.
• the pH is measured using a very sensitive pH probe and meter.
• the meter displays the pH to 3 significant figures to the right of the decimal. There is a separate temperature probe with meter and both must be in the solution and stable. This pH meter is capable of a 5 point calibration curve with 3 points being an absolute minimum.
• the pH of the stabilizer solution is adjusted to the target pH prior to adding the virus antigen, and the pH is measured again after mixing the stabilizer solutions and virus antigens.
• the pH is confirmed /adjusted to pH 7.2 at the desired temperature (e.g., 4°, 15°, or 25°C), i.e., for many experiments performed herein the desired temperature was 4°C. If the formulation and previous procedures have been performed correctly the pH should be close to target pH. With an overnight incubation the pH may drift slightly due to the completion of chemical reactions associated with the earlier pH adjustment and further de-gassing.
• the desired temperature e.g., 4°, 15°, or 25°C
• Thawing virus bulk antigen The frozen antigens are slowly thawed at room temperature (15- 30°C) or at refrigerated temperature (2-8°C). The thawed antigen should be kept at 2-8°C for no more than 8 hours prior to usage.
• Preparation of vaccine blend To make a 100 mL of vaccine blend, the volume of each stock solution required to reach the final concentration for each component as listed in Table 2B was calculated and then added to a sterilized container first, and the stabilizers and excipients were mixed using a stirring bar. After the stabilizer solutions and all excipients were thoroughly mixed, the virus antigens at the indicated volume were added into the container and thoroughly mixed. The generating of bubbles and foams were avoided during this mixing step. When the virus is added in a very short time frame (a few minutes) then the virus may be added with no further issues. When the virus is not immediately added, an argon overlay is put in place to displace residual 0 2 .
• argon gas is added to the bottle using a low flow rate.
• the vaccine blend is kept at 2-8°C until dispensing into small aliquots.
• the vaccine blend was dispensed into glass ampule vials at 1.8 mL per vial. Each vial is then back-filled and overlaid with argon gas. The ampule vials were flame sealed, labelled and then transferred into boxes, and stored in the incubator at the designated temperature. Stability testing at elevated temperature and real-time conditions: Liquid PRRS vaccines in ampule vials were stored at 27°C and 4°C respectively in the corresponding incubators. At the designated time point, 2 or more vials from each formulation were retrieved and the titer of each antigen was measured by tissue cell culture based virus titration assay.
• PRRSv titration assay The infectivity of test samples containing PRRSv is determined by titration on susceptible tissue culture cells, preferably African Green Monkey kidney cell lines such as MARC145 or MA104. Three to four days prior to the assay, cells are seeded into suitable culture plates or dishes, such as 96-well tissue culture plates.
• Actively growing cells are seeded at 5x10 4 cells/well in assay media (for instance Dulbecco's Minimum Essential Media, supplemented with 10 mM HEPES, 2 mM L-Glutamine, 60 ⁇ g/mL Gentamycin and 7% fetal bovine serum), and incubated at 37°C under 5% C0 2 atmosphere in a humidified incubator until use in the assay.
• assay media for instance Dulbecco's Minimum Essential Media, supplemented with 10 mM HEPES, 2 mM L-Glutamine, 60 ⁇ g/mL Gentamycin and 7% fetal bovine serum
• appropriate serial dilutions of the test samples are prepared in assay media in dilution tubes, for instance glass tubes, or polypropylene tubes.
• 10-fold dilutions may be prepared by adding 0.2 mL of undiluted test sample to 1.8 mL of assay media, briefly mixing by means of a vortex mixer, transferring 0.2 mL of the mixture into a separate test tube containing 1.8 mL of assay media, and so on.
• test sample For each test sample, one 96-well plate is removed from incubation, and the media is removed by inverting the plate and aseptically 'dumping' the media into a suitable receptacle. The sample is tested by adding 0.1 mL of each dilution to each of 10 wells on the plate. Dilutions are added from the highest dilution to the lowest dilution to be tested. Incubation is then continued for an additional 7 days.
• the plates may be read either by visually observing cytopathic effects (CPE) using a light microscope at 100-lOOOx magnification, or macroscopically after staining with Crystal Violet (CV).
• CPE cytopathic effects
• CV Crystal Violet
• a stock solution is prepared by dissolving 5 g of CV powder per 100 mL of 95% ethanol, followed by the addition of an equal volume of formaldehyde (37% stock solution), and the addition of deionized or better quality water to a final volume of 1 ,000 mL per 100 mL of ethanol.
• Media are removed from the 96-well plates by 'dumping', and CV stock solution is carefully added at 0.15 mL/well without disturbing remaining monolayers.
• the plate is left at ambient temperature for 15 minutes, after which the CV solution is removed by 'dumping'.
• the wells are then carefully rinsed under gently running tap water. Finally, the water is removed by 'dumping'.
• Virus titers are calculated according to the method of Spearman and Karber, and are expressed as Logio TdD 5 o/mL.
• Table 2B below lists 10 liquid stable formulations for swine virus vaccines of the present invention.
• Formulation 11 is a tryptone, lactose formulation that was designed in view of the freeze-drying methodology that was employed heretofore to stabilize live swine virus vaccines.
• the standard freeze drying formulation (Formulation 1 1) started to fail immediately, see Table 3 below.
• the titer of all of the formulations were comparable to formulation 2 (see, Table 2B below).
• the titer of all of the 10 variations of this formulation appear to remain relatively stable at 2°C -7°C for at least 6 months, the final time point measured.
• the titer of formulation 1 1 is measurably decreasing at this 6 month time point, having lost close to 1.5 logs of in its titer, see, Table 4 below.
• N/A indicates that the pH was not adjusted.
• sorbitol Sorb.
• arginine ARG
• methionine MET
• glutamic acid GLU
• ethylenediaminetetraacetic acid EDTA
• Pluronic F-68 F-68
• polyvinylpyrrolidone K- 60 K-60 PVP
• Formulation 11 comprised: 3.75% (w/v) Bacto Tryptone; 1.5% (w/v) dextran; 0.1% (w/v) gelatin; 5.0% (w/v) lactose; 0.1% (w/v) sodium glutamate; 0.5% (w/v) albumin Fraction V, and buffered with monobasic and dibasic potassium phosphate.

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