WO2015053386A1 - 培養装置 - Google Patents
培養装置 Download PDFInfo
- Publication number
- WO2015053386A1 WO2015053386A1 PCT/JP2014/077158 JP2014077158W WO2015053386A1 WO 2015053386 A1 WO2015053386 A1 WO 2015053386A1 JP 2014077158 W JP2014077158 W JP 2014077158W WO 2015053386 A1 WO2015053386 A1 WO 2015053386A1
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- WO
- WIPO (PCT)
- Prior art keywords
- inner box
- shelf
- transfer sheet
- heat transfer
- side plate
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
- B01L1/02—Air-pressure chambers; Air-locks therefor
- B01L1/025—Environmental chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/06—Plates; Walls; Drawers; Multilayer plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/025—Align devices or objects to ensure defined positions relative to each other
Definitions
- the present disclosure relates to a culture apparatus.
- a culture apparatus having a heater for example, Patent Document 1.
- the heater is arranged outside the inner box.
- the heat of the heater may be difficult to be transmitted to a portion of the inner box away from the heater.
- the culture apparatus includes an outer box, an inner box made of a metal plate arranged inside the outer box, a heater arranged outside the inner box, the outer box, and a front surface of the inner box.
- a device that opens and closes the opening to be formed, and cultivates the object to be cultured inside the inner box, which is formed by pressing on both side plates of the inner box, and the object to be cultured is mounted
- a shelf holder on which both side portions of the bottom surface of the shelf plate to be placed are mounted, an outer surface of at least one side plate of the inner box, and a heat transfer sheet for transmitting heat of the heater to the inner box,
- the heat transfer sheet is attached to an outer surface of the side plate of the inner box, and is also attached to a first recess formed on the outer side of the side plate of the inner box by forming the shelf support.
- a plurality of first cuts for bending the heat transfer sheet are intermittently provided.
- the heat of the heater can be transmitted to the inner box.
- FIG. 1 is a perspective view showing a culture apparatus in the present embodiment.
- FIG. 2 is a perspective view showing the culture apparatus in a state where the outer door and the inner door are opened in the present embodiment.
- FIG. 3 is a cross-sectional view showing the culture apparatus in the present embodiment.
- FIG. 4 is a cross-sectional view showing the culture apparatus with the outer door omitted in the present embodiment. 3 and 4 show the culture device 100 in a state viewed in the + X direction from a cross section parallel to the ZY plane passing through the approximate center of the culture device 100.
- FIG. 1 is a perspective view showing a culture apparatus in the present embodiment.
- FIG. 2 is a perspective view showing the culture apparatus in a state where the outer door and the inner door are opened in the present embodiment.
- FIG. 3 is a cross-sectional view showing the culture apparatus in the present embodiment.
- FIG. 4 is a cross-sectional view showing the culture apparatus with the outer door omitted in the present embodiment. 3 and 4 show the culture
- the X axis is an axis orthogonal to the side plates 12 and 13 of the outer box 10, and the direction from the side plate 12 to the side plate 13 is + X, and the direction from the side plate 13 to the side plate 12 is -X.
- the Y axis is an axis orthogonal to the outer door 11 and the back plate 14, and the direction from the outer door 11 toward the back plate 14 is defined as + Y, and the direction from the back plate 14 toward the outer door 11 is defined as -Y.
- the Z axis is an axis orthogonal to the bottom plate 15 and the top plate 16, and the upward direction from the bottom plate 15 to the top plate 16 is + Z, and the downward direction from the top plate 16 to the bottom plate 15 is -Z.
- the culture apparatus 100 is an apparatus for culturing an object to be cultured such as cells and microorganisms in the culture chamber 2A.
- the culture apparatus 100 includes an outer box 10, an outer door 11, an inner box 20 (FIG. 3), an inner door 21, a water tray 402, a shelf board 3, a heater device 142 (FIG. 3), and heat transfer sheets 9, 9A, 9B ( 6).
- the outer box 10 and the inner box 20 are substantially rectangular parallelepiped boxes made of a metal material such as stainless steel.
- the outer shape of the inner box 20 is smaller than the inner shape of the outer box 10 so that the inner box 20 is accommodated inside the outer box 10.
- a space 111 (FIG. 3) filled with air that functions as a heat insulating material is formed.
- the space 111 may be filled with a predetermined heat insulating material other than air.
- an opening 21A that leads to the culture chamber 2A is formed on the front surface (-Y) of the outer box 10 and the inner box 20.
- the outer door 11 and the inner door 21 are doors that open and close the opening 21A.
- the outer door 11 is made of a metal material such as stainless steel, has a substantially rectangular shape whose outer shape is larger than that of the inner door 21, and is filled with a heat insulating material.
- a packing 110 for ensuring airtightness in the culture apparatus 100 is provided on the peripheral edge of the outer door 11 adjacent to the inner door 21.
- On the opposite side of the outer door 11 from the inner door 21, an operating device 143 for operating the culture device 100 is provided.
- the inner door 21 is a member made of a transparent material such as resin or glass.
- a packing 210 for ensuring airtightness in the culture apparatus 100 is provided at a portion of the edge of the opening 21 ⁇ / b> A on the front surface of the outer box 10 that is opposed to the inner door 21.
- the shelf board 3 has a substantially rectangular shape when viewed from the + Z side toward the ⁇ Z side, and is made of a metal material such as stainless steel on which the culture object is placed. Further, the shelf board 3 is placed on a plurality of shelf supports 5 and 6 provided in the inner box 20. One shelf plate 3 may be provided in the inner box 20 or a plurality of shelf plates 3 may be provided.
- the water tray 402 is a container for storing water generated when the culture chamber 2A is dehumidified, and is placed on the bottom plate 25 of the inner box 20.
- the heater device 142 is a device that adjusts the temperature of the culture chamber 2 ⁇ / b> A, and is provided in the case 141.
- the heater device 142 operates by operating the operation device 143, and dissipates heat from the heater wire 900 (FIG. 14).
- the heater wire 900 (heater) is affixed to the heat transfer sheets 9, 9A, 9B, and the heat dissipated from the heater wire 900 is transferred to the inner box 20 and the heat transfer sheets 9, 9A, 9B (FIG. 6). It is transmitted to the culture room 2A.
- the heat transfer sheets 9, 9 ⁇ / b> A, 9 ⁇ / b> B are made of a material such as aluminum having a relatively high thermal conductivity, and transmit the heat dissipated from the heater wire 900 or the like to the inner box 10.
- FIG. 5 is a front view showing the culture apparatus in a state where the outer door and the inner door are omitted in the present embodiment.
- FIG. 6 is a cross-sectional view showing the inner box and the outer box in the present embodiment.
- FIG. 5 is a front view showing the culture apparatus in a state where the outer door and the inner door are omitted in the present embodiment.
- FIG. 6 is a cross-sectional view showing the inner box and the outer box in the present embodiment.
- FIG. 6 shows the inner box 20 and the outer box 10 as viewed in the + Y direction from a cross section parallel to the ZX plane passing through the approximate center of the culture apparatus 100 in FIG.
- the side plates 22 and 23, the back plate 24, the bottom plate 25, and the top plate 26 of the inner box 20 are shown as flat plates.
- the inner box 20 has side plates 22 and 23, a back plate 24, a bottom plate 25, and a top plate 26.
- the side plates 22, 23, the bottom plate 25, and the top plate 26 are formed by welding four metal plates 72, 73, 75, 76.
- the metal plates 72 and 73 are plate members for forming the side plates 22 and 23 by being pressed so that, for example, the plurality of shelf supports 5 and 6 are provided in the inner box 20.
- the metal plate 76 is a plate member for forming the top plate 26. Both ends of the metal plate 76 are bent so that the joint portions 721 and 731 are closer to the side plates 22 and 23 than the corner portions 201 and 202 of the substantially rectangular parallelepiped shape of the inner box 20.
- the metal plate 75 is a plate member for forming the bottom plate 25. Both ends of the metal plate 75 are bent so that the joint portions 722 and 732 are closer to the side plates 22 and 23 than the corner portions 203 and 204 of the substantially rectangular parallelepiped shape of the inner box 20.
- FIG. 7 is a perspective view showing a shelf holder in the present embodiment.
- FIG. 8 is a front view showing a shelf holder in the present embodiment.
- FIG. 9 is a side view showing the shelf holder in the present embodiment.
- FIG. 10 is a side view showing the shelf support in a state where the shelf board in this embodiment is placed.
- the inside of the inner box 20 has a symmetrical shape with respect to a symmetry plane that passes through the center of the inner box 20 and is parallel to the YZ plane.
- the inside of the inner box 20 is chamfered with corners so that cleaning is easy.
- Shelf holder The shelf boards 3 are placed on the plurality of shelf supports 5 and 6.
- the shelf receivers 51 to 59 of the plurality of shelf receivers 5 respectively support the shelf boards 3 in pairs with the shelf receivers 61 to 69 of the plurality of shelf receivers 6 so that the shelf boards 3 are substantially horizontal. Since the shelf receivers 51 to 59 of the plurality of shelf receivers 5 have the same configuration as the shelf receivers 61 to 69 of the plurality of shelf receivers 6, only the plurality of shelf receivers 5 will be described. The description of is omitted.
- the plurality of shelf supports 5 are formed by pressing the side plate 23.
- the plurality of shelf supports 5 are provided on the raised portions 231 of the side plate 23.
- the raised portion 231 is a part of the side plate 23 that is pressed so as to rise in the direction ( ⁇ X) away from the side plate 13 of the outer box 10.
- the raised portion 231 is raised to the center ( ⁇ X) of the inner box 20 rather than the packing 210 (FIG. 9) as the edge of the opening 21A.
- the shelf boards 3 placed on the plurality of shelf supports 5 are reliably positioned.
- the plurality of shelf supports 5 have shelf supports 51 to 59 provided so as to be arranged in the vertical direction (Z axis).
- the distance between, for example, the shelf receiver 51 as the upper (+ Z) shelf receiver adjacent in the vertical direction and the shelf receiver 52 as the lower ( ⁇ Z) shelf receiver is the shelf mounted on the shelf receiver 52.
- the distance is set to limit the vertical movement of the plate 3 to a certain amount.
- the fixed amount is, for example, a distance that allows the shelf 3 to move in the front-rear (Y-axis) direction when the shelf 3 is lifted upward in the vertical direction. It may be about a few centimeters. Since the shelf receivers 51 to 59 have the same configuration, only the shelf receiver 51 will be described, and the description of the configuration of the shelf receivers 52 to 59 will be omitted.
- the shelf holder 51 protrudes in a direction away from the side plate 13 of the outer box 10 and has a continuous long shape extending from the inner door 21 side (-Y) to the back plate 24 side (+ Y).
- the shelf holder 51 includes a placement surface 511, an inclined surface 515, and stoppers 51A and 51B.
- the side surface 33 of the bottom plate 31 of the shelf board 3 is placed on the mounting surface 511.
- the mounting surface 511 is inclined toward the bottom plate 25 as it proceeds in the direction ( ⁇ X) away from the side plate 23 of the inner box 20.
- the placement surface 511 is formed such that the placed shelf 3 is horizontal.
- the inclined surface 515 is inclined from the front end 517 of the shelf receiver 51 toward the lower side ( ⁇ Z) and toward the side plate 23 so that the volume of the culture chamber 2A is increased.
- the tip 517 of the shelf receiver 51 is a part of the shelf receiver 51 corresponding to the position farthest from the side plate 23 in the X-axis direction.
- the inclined surface 515 is provided on the lower side ( ⁇ Z) of the mounting surface 511. And the thickness in the vertical direction of the mounting surface 511 and the inclined surface 515 becomes thinner as the distance from the side plate 23 increases.
- the stoppers 51 ⁇ / b> A and 51 ⁇ / b> B are provided integrally with the shelf receiver 51 so as to limit the vertical movement of the shelf plate 3 placed on the shelf receiver 51.
- the stoppers 51 ⁇ / b> A and 51 ⁇ / b> B are provided at positions separated along the longitudinal direction (Y axis) of the shelf receiver 51. Since the stoppers 51A and 51B have the same configuration, only the stopper 51A will be described, and the description of the stopper 51B will be omitted.
- the stopper 51A is provided on the inclined surface 515 on the lower side ( ⁇ Z) than the placement surface 511.
- the stopper 51 ⁇ / b> A has a first inclined surface 512, a second inclined surface 513, and a third inclined surface 514.
- the first inclined surface 512 is in contact with the side portion 33 of the shelf board 3 and restricts the movement of the shelf board 3 in the vertical direction.
- the first inclined surface 512 is provided below the inclined surface 515 (FIG. 9).
- the first inclined surface 512 is inclined so that the minor angle between the first inclined surface 512 and the side plate 23 is larger than the minor angle between the inclined surface 515 and the side plate 23.
- the second inclined surface 513 is inclined to prevent the shelf board 3 from being caught by the stopper 51 ⁇ / b> A when the shelf board 3 is put in the inner box 20.
- the second inclined surface 513 is inclined so as to go in the + Y direction as the distance from the side plate 23 increases.
- the third inclined surface 514 is inclined to prevent the shelf board 3 from being caught by the stopper 51 ⁇ / b> A when the shelf board 3 is taken out of the inner box 20.
- the third inclined surface 514 is inclined so as to be in the ⁇ Y direction as the distance from the side plate 23 increases.
- Projection In order to take out the water tray 402 (FIG. 4), it is necessary to lift the end portion ( ⁇ Y) near the opening 21A in the water tray 402 so that the height in the vertical direction is higher than the part 2D of the packing 210. There is. For example, when a relatively large amount of water is stored in the water tray 402, the water in the water tray 402 may spill when the ⁇ Y side end of the water tray 402 is suddenly lifted. is there. A part 2D of the packing 210 is a part provided on the lower edge of the opening 21A in the packing 210.
- the protrusion 401 is used to take the water tray 402 out of the inner box 20 and the outer box 10 through the opening 21A.
- the protrusion 401 is formed by pressing the bottom plate 25 so as to protrude toward the upper side (+ Z).
- the protrusion 401 is provided near the opening 21A (-Y) in the bottom plate 25.
- the protrusion 401 has an elongated shape that continues along the X axis.
- the protrusion 401 has a mountain shape with a predetermined height. The height of the protrusion 401 is such that when the water dish 402 is taken out, the water dish 402 can be guided so that the end of the ⁇ Y side of the water dish 402 is positioned higher than a part 2D of the packing 210. Is set.
- FIG. 10 is a side view showing the shelf support in a state where the shelf board in this embodiment is placed.
- FIG. 11 is a diagram showing a culture device and a shelf board in the present embodiment.
- FIG. 11 shows a plan view of the shelf board.
- FIG. 11 further shows the culture apparatus 100 as viewed in the ⁇ Z direction from a cross section passing between the shelf supports 51 and 52 in the vertical direction (Z axis) of FIG. 5 and parallel to the XY plane.
- the shelf board 3 (FIG. 11) has a symmetrical shape with respect to a symmetry plane that passes through the approximate center of the shelf board 3 and is parallel to the YZ plane.
- the shelf plate 3 has a bottom plate 31 and side portions 32 and 33 and is integrally formed by bending a single metal plate.
- the bottom plate 31 is a flat plate having a substantially rectangular shape.
- the bottom plate 31 is provided with a plurality of punch holes 31A for circulating the gas in the culture chamber 2A.
- the width D1 of the bottom plate 31 in the X-axis direction is set shorter than the width D2 from the ⁇ X side surface of the raised portion 231 to the raised portion 221 of the side plate 22 in the X-axis direction.
- the side portion 33 includes a first bent piece 331 (FIG. 10) and a second bent piece 332.
- the first bent piece 331 is bent from the + X side end of the bottom plate 31 toward the upper side (+ Z).
- the first bent piece 331 is bent so that the curvature of the curved portion 333 is larger than the curvature of the curved portion 521A continuous from the surface (-X) on the culture chamber 2A side in the raised portion 231 to the placement surface 521.
- the length of the first bent piece 331 in the vertical direction (Z-axis) is set to be shorter than the distance from the mounting surface 521 to the first inclined surface 512 in the vertical direction.
- the second bent piece 332 is further bent from the first bent piece 331.
- the second bent piece 332 is bent from the upper end of the first bent piece 331 toward the center side ( ⁇ X) of the inner box 20.
- the second bent piece 332 is bent along the first inclined surface 512 of the stopper 51A and the first inclined surface 516 of the stopper 51B.
- FIG. 12 is a view showing a heat transfer sheet in the present embodiment.
- FIG. 13 is a diagram showing a side plate and a heat transfer sheet of the inner box in the present embodiment.
- FIG. 12 is a view showing a heat transfer sheet in the present embodiment.
- FIG. 13 is a diagram showing a side plate and a heat transfer sheet of the inner box in the present embodiment.
- FIG. 14 is a diagram showing a side plate, a heat transfer sheet, and a heater wire of the inner box in the present embodiment.
- 13 and 14 show the heat transfer sheet 9 attached to the side plate 13 side (outside) surface (+ X) of the side plate 23.
- FIG. 15 is a cross-sectional view showing a shelf holder and a heat transfer sheet in the present embodiment.
- FIG. 15 shows the side plate 23 and the like viewed from the cross section including the line segment Z1 of FIG. 14 and parallel to the XZ plane toward the + Y direction.
- FIG. 16 is a cross-sectional view showing the stopper and the heat transfer sheet in the present embodiment.
- FIG. 17 is a cross-sectional view showing the stopper and the heat transfer sheet in a state where the heat transfer sheet is pushed into the recess according to the present embodiment.
- 16 and 17 show the side plate 23 and the like viewed from the cross section including the line segment Z2 of FIG. 14 and parallel to the XZ plane in the + Y direction.
- FIG. 16 shows the heat transfer sheet 9 in a state where it is not pushed into the recess 519.
- FIG. 17 shows the heat transfer sheet 9 in a state of being pushed into the recess 519.
- Configuration of heat transfer sheet The heat transfer sheets 9, 9 ⁇ / b> A, 9 ⁇ / b> B transmit the heat dissipated from the heater wire 900 to the inner box 20.
- the heat transfer sheet 9 is attached to the side plate 13 side (outside) surface (+ X) of the side plate 23.
- the heat transfer sheet 9A is attached to the surface ( ⁇ X) of the side plate 22 on the side plate 12 side (outside).
- the heat transfer sheet 9B is affixed to a surface ( ⁇ X) on the top plate 16 side (outside) of the top plate. Since the configurations of the heat transfer sheets 9 and 9A are the same, only the configuration of the heat transfer sheet 9 will be described, and the description of the configuration of the heat transfer sheet 9A will be omitted.
- the heat transfer sheet 9 has a substantially rectangular shape.
- the area of the heat transfer sheet 9 is set smaller than the area of the outer surface of the side plate 23 so that the heat transfer sheet 9 can be attached to the side plate 23.
- Each notch and each extraction hole are provided in the first position 91 to the ninth position 99 of the heat transfer sheets 9 adjacent in the Z-axis direction.
- the first position 91 to the ninth position 99 correspond to the positions of the recesses formed on the outer side of the side plate 23 by pressing for forming the shelf supports 51 to 59, respectively.
- the first position 91 corresponds to the position of the recess 518 (FIG. 15) of the side plate 23 that is recessed to form the shelf holder 51.
- Each notch and each extraction hole are used for sticking the heat transfer sheet 9 inside each recess of the side plate 23.
- or the 9th position 99 are the same structures, the notch provided in the 1st position 91 is provided. Only the extraction holes will be described, and the description of the cuts and extraction holes provided in the second position 92 to the ninth position 99 will be omitted.
- a plurality of cuts 911 to 917 (first cut and second cut) formed by cutting along the Y axis are provided intermittently along the Y axis.
- the notches 911 to 917 are such that after the heat transfer sheet 9 is affixed to the outer surface of the side plate 23, parts 901 and 902 (FIGS. 15 and 16) of the heat transfer sheet 9 are inside the recess 518 (first recess). It is used to bend the heat transfer sheet 9 so as to be affixed to.
- the cuts 911, 913, 915, and 917 have the same length.
- the notches 912, 914, and 916 are provided between the notches 911 and 913, between the notches 913 and 915, and between the notches 915 and 917, respectively, and shorter than the notches 911, 913, 915, and 917. Is set. Further, no notch is provided along the Y-axis direction between the notch 911 and the notch 912, and is continuous from the + Z side of the notches 911 to 917 to the ⁇ Z side, and from the + Z side of the notches 911 to 917. A connecting portion 91C connecting the heat transfer sheet 9 on the ⁇ Z side is provided.
- a connecting portion 91D having the same configuration as the connecting portion 91C is provided between the notches 912 and 913, and connecting portions having the same configuration as the connecting portion 91C are provided between the notches 913 to 917. Will be. Therefore, at the first position 91, three pairs of connection portions including the connection portions 91C and 91D are provided along the Y axis. The heat transfer sheet 9 is reinforced by these three pairs of connecting portions. Further, reinforcing holes 91A and 91B for reinforcing the heat transfer sheet 9 together with the connecting portions 91C and 91D are provided at both ends of the notch 911.
- the heat transfer sheet 9 is affixed with an adhesive such that it can be peeled off from a base plate 90 for maintaining the shape of the heat transfer sheet 9 before being attached to the side plate 23.
- the heat transfer sheet 9 is not peeled off from the mount 90 due to the adhesive force of the adhesive, and the heat transfer from the end of the notch is based on the force applied to the heat transfer sheet 9.
- the sheet 9 may be torn.
- the heat transfer sheet 9 is reinforced by the connecting portions 91 and 91D and the reinforcing holes 91A and 91B, the heat transfer sheet 9 can be reliably peeled without being broken when the heat transfer sheet 9 is peeled from the mount 90. Become.
- a pair of extraction holes 918 and 919 is provided at the first position 91.
- the extraction holes 918 and 919 are used to extract gas generated between the heat transfer sheet 9 and the outer surface of the side plate 23 when the heat transfer sheet 9 is attached to the outer surface of the side plate 23.
- the extraction hole 918 is provided at a position facing a recess 519 (FIG. 16) formed on the outside of the side plate 23 by press working for forming the stopper 51A.
- the recess 519 is formed by further recessing the recess 518.
- the extraction hole 919 is also provided at a position facing a depression formed on the outside of the side plate 23 by press working for forming the stopper 51 ⁇ / b> B.
- the extraction hole 918 is provided in the vicinity of the connection portions 91 ⁇ / b> C and 91 ⁇ / b> D in order to improve the strength of the heat transfer sheet 9. Similar to the extraction hole 918, the extraction hole 919 is provided in the vicinity of the connection portion between the cuts 915 and 916 and the connection portion between the cuts 916 and 917.
- Attaching the heat transfer sheet to the side plate The mount 90 of the heat transfer sheet 9 is peeled off, and the surface to which the adhesive is applied is attached to the outer surface of the side plate 23 (FIG. 13).
- the heat transfer sheet 9 is not affixed inside the recesses 518 and 519 (FIGS. 15 and 16).
- the heat transfer sheet 9 is pushed along the recess 518 so that a part 901, 902, etc. of the heat transfer sheet 9 is stuck inside the recess 518 and the recess 519 (second recess).
- the connecting portions 91C, 91D, etc. are pushed and cut, so that the cuts 911 and 912, the cuts 912 and 913 are connected, and the long cuts become a release end and are easy to push into the recess 518, etc. Is bent and pushed toward the ⁇ X side (FIGS. 15 and 16).
- the heat transfer sheet is not attached to the inside of the recess 519 (FIG.
- FIG. 18 is a side view showing the shelf board and the culture apparatus in the present embodiment.
- the side plates 22 and 12 in the culture apparatus 100 are omitted.
- shelves can be inserted The outer door 11 and the inner door 21 are opened, and the shelf board 3 can be put into the inner box 20 through the opening 21A.
- the shelf board 3 moves from the front side ( ⁇ Y) to the rear side (+ Y) between the shelf receivers 52 and 62 and the shelf receivers 51 and 61 so as to be placed on the shelf receivers 52 and 62. Is done.
- the movement of the side portion 33 is guided by the placement surface 521 (FIG. 10) below ( ⁇ Z) the shelf plate 3 and the inclined surface 515 above the shelf plate 3.
- the shelf 3 is prevented from being caught by the stopper 51A due to the inclination of the second inclined surface 513 of the stopper 51A.
- the side portion 32 is guided in the same manner as the side portion 33.
- the shelf board 3 moves to the inside of the inner box 20 and is placed on the shelf receivers 52 and 62. At this time, since the mounting surface 521 is inclined, the side portion 33 of the shelf board 3 comes into line contact with the shelf receiver 52. Therefore, the inside of the inner box 20 can be reliably sterilized by filling the culture chamber 2A with the sterilizing gas.
- the side portion 32 is also in line contact with the shelf holder 62 in the same manner as the side portion 33.
- the curvature of the curved part 333 of the side part 33 is set larger than the curvature of the curved part 521A. In order to slide down the mounting surface 521 so that the shelf board 3 is positioned at a predetermined position, the shelf board 3 is reliably positioned in the X-axis direction.
- the inner door 21 and the outer door 11 are closed.
- a shelf board is taken out The outer door 11 and the inner door 21 are opened, and the shelf plate 3 in the inner box 20 can be taken out through the opening 21A.
- the shelf board 3 placed on the shelf receivers 52 and 62 is moved from the rear side to the front side. Since the movement in the vertical direction is restricted by the stoppers 51A and 52A, for example, it is possible to prevent the shelf board 3 from being tilted and falling off the shelf board 3 while the shelf board 3 is moving.
- the inclination of the shelf 3 indicates that the front end of the shelf 3 is inclined so as to be lower than the rear end. Further, since the side portion 33 of the shelf board 3 comes into contact with the first inclined surfaces 512 and 516 of the stoppers 51A and 51B provided on the upper shelf support 51, the inclination angle of the shelf board 3 is further increased. This prevents the shelves 3 from falling off.
- the culture apparatus 100 is an apparatus for cultivating an object to be cultured inside the inner box 20.
- the culture apparatus 100 includes an outer box 10, an inner box 20, an outer door 11, and an inner door 21.
- the inner box 20 includes metal plates 72, 73, 75, 76 and the like disposed inside the outer box 10.
- the outer door 11 and the inner door 21 open and close the opening 21A.
- the culture apparatus 100 further includes shelf holders 5 and 6 and a heat transfer sheet 9.
- the shelf supports 5 and 6 are formed on the side plates 22 and 23 of the inner box 20 by pressing, and both side portions 32 and 33 of the bottom plate 31 (bottom surface) of the shelf plate 3 on which the culture object is placed are placed. .
- the heat transfer sheet 9 is affixed to the outer surface of the side plate 23 of the inner box 20 and transfers the heat of the heater wire 900 to the inner box 20. After the heat transfer sheet 9 is affixed to the outer surface of the side plate 23 of the inner box 20, it is also affixed to the recesses 518 and 519 formed on the outer side of the side plate 23 of the inner box 20 by forming the shelf holder 51. As described above, the heat transfer sheet 9 is intermittently provided with a plurality of cuts 911 to 917, extraction holes 918, and extraction holes 919. Therefore, the heat of the heater wire 900 can be reliably transmitted via the heat transfer sheet 9 to a position away from the heater wire 900 on the side plate 23 of the inner box 20.
- the support pillar etc. for supporting the shelf board 3 are not provided in the inside of the inner box 20, the inside of the inner box 20 becomes easy to clean. Moreover, since it is not necessary to provide the support pillar etc. for supporting the shelf board 3 in the inner box 20, the number of parts of the culture apparatus 100 can be reduced and the manufacturing cost of the culture apparatus 100 can be reduced. .
- the heat transfer sheet 9 has an extraction hole 918 for extracting gas generated when the heat transfer sheet 9 is attached to a surface including the depressions 518 and 519 on the outer side of the side plate 23 of the inner box 20.
- the heat transfer sheet 9 can be placed along the recess 519, and the contact area between the heat transfer sheet 9 and the side plate 23 is increased, so that the heat of the heater wire 900 is reliably transmitted to the inner box 20. Can do.
- the shelf holder 51 has a long shape.
- the cuts 911 to 917 are formed by cutting along the longitudinal direction (Y axis) of the shelf holder 51.
- the side plate 23 of the inner box 20 is further formed with a stopper 51A for further recessing the recess 518 by press working to limit the vertical movement of the shelf plate 3 placed on the shelf support 52. .
- the extraction hole 918 is provided at a position facing the recess 519 formed inside the recess 518 when the stopper 51A is formed.
- the heat transfer sheet 9 is bent so as to be attached to the recess 519 together with the recess 518. Accordingly, when the stopper 51A is further formed on the shelf holder 51, it is extracted to a position facing the depression 519 in order to discharge the gas in the space surrounded by the adhesive of the heat transfer sheet 9 around the stopper 51A. Holes 918 will be provided. Therefore, the heat of the heater wire 900 can be reliably transmitted to the inner box 20 by reliably extracting the gas generated when the inner box 20 is attached to the side plate 23.
- the said embodiment is for making an understanding of this indication easy, and is not for limiting and limiting this indication.
- the present disclosure can be changed and improved without departing from the gist thereof, and the present disclosure includes equivalents thereof.
- the heater wire 900 is provided between the outer box 10 and the inner box 20 and heat is dissipated from the heater wire 900, but the present invention is not limited to this.
- a cooling device including an evaporator that absorbs heat may be provided between the box 10 and the inner box 20, and the heat of the culture chamber 2A may be absorbed by the cooling device via the inner box 20 and the heat transfer sheet 9.
- both the heater wire 900 and the evaporator may be provided between the outer box 10 and the inner box 20, and heat may be radiated from the heater wire 900 or absorbed by the evaporator.
- heat-transfer sheets 9, 9A, and 9B were affixed with respect to the inner box 10, it is not limited to this.
- a heat transfer sheet may be attached to the surface (-Z) of the bottom plate 25 on the bottom plate 15 side. Further, for example, only at least one of the heat transfer sheets 9 and 9A may be attached.
- an adhesive may be applied to the outer surface of the side plate 23, or an adhesive may be applied to both the heat transfer sheet 9 and the side plate 23.
- the extraction hole 918 is provided at a position facing the recess 519, but the present invention is not limited to this.
- the extraction hole 918 may be provided at a position facing the recess 518, or may be provided at a position facing the side plate 23.
- two stoppers 51A and 51B are provided in the longitudinal direction (Y axis) of the shelf holder 51, but the present invention is not limited to this.
- one stopper having the same configuration as the stopper 51 ⁇ / b> A in the longitudinal direction of the shelf receiver 51 may be provided, or three or more stoppers may be provided.
- one stopper may have a long shape along the longitudinal direction (Y axis) of the shelf support 51.
- the stoppers 51A and 51B are provided on the inclined surface 515.
- the present invention is not limited to this.
- the stoppers 51A and 51B may be provided from the inclined surface 515 to the raised portion 231 of the side plate 23 on the lower side of the inclined surface 515 (-Z in FIG. 7).
- the joint portions 721 and 731 are closer to the side plates 22 and 23 than the corner portions 201 and 202 of the inner box 20, and the joint portions 722 and 732 are closer to the side plates than the corner portions 203 and 204 of the inner box 20.
- the inner box 20 is formed so as to be closer to 22, 23, the present invention is not limited to this.
- the inner box 20 is formed so that the joint portions 721 and 731 are closer to the top plate 26 than the corner portions 201 and 202, and the joint portions 722 and 732 are closer to the bottom plate 25 than the corner portions 203 and 204. Also good.
- the 1st bending piece 331 was bent so that the curvature of the curved part 333 (FIG. 10) might become larger than the curvature of the curved part 521A, it is limited to this. is not.
- the first bent piece 331 may be bent so that the curvature of the curved portion 333 is smaller than the curvature of the curved portion 521A.
- a gap is formed between the side portion 33 of the shelf board 3 placed on the placement surface 521, the raised portion 231, and the placement surface 521. The sterilization gas in the culture chamber 2A is allowed to enter the gap so that the culture chamber 2A can be sterilized reliably.
- the protrusion 401 (FIG. 4) was exhibiting the elongate shape which followed the X-axis, it is not limited to this.
- a plurality of protrusions that exhibit the same function as the protrusion 401 may be provided along the X axis, or one protrusion may be provided.
- the metal plate was bent and the shelf board 3 was formed integrally, it is not limited to this.
- the side plates 32 and 33 may be welded to the bottom plate 31 to form the shelf plate 3.
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Abstract
Description
===培養装置===
以下、図1乃至図4を参照して、本実施形態における培養装置について説明する。図1は、本実施形態における培養装置を示す斜視図である。図2は、本実施形態における外扉及び内扉が開かれた状態の培養装置を示す斜視図である。図3は、本実施形態における培養装置を示す断面図である。図4は、本実施形態における外扉が省略された状態の培養装置を示す断面図である。図3及び図4は、培養装置100の略中央を通るZY平面に平行な断面から+X方向へ見た状態の培養装置100を示している。
===内箱===
以下、図3、図5及び図6を参照して、本実施形態における内箱について説明する。図5は、本実施形態における外扉及び内扉が省略された状態の培養装置を示す正面図である。図6は、本実施形態における内箱及び外箱を示す断面図である。尚、図6は、図1における培養装置100の略中央を通るZX平面に平行な断面から+Y方向へ見た状態の内箱20及び外箱10を示している。又、説明の便宜上、内箱20における側板22、23、背板24、底板25、天板26が夫々平面板として示されている。
===内箱の内部===
以下、図4、図5、図6乃至図10を参照して、本実施形態における内箱の内部について説明する。図7は、本実施形態における棚受を示す斜視図である。図8は、本実施形態における棚受を示す正面図である。図9は、本実施形態における棚受を示す側面図である。図10は、本実施形態における棚板が載置されている状態の棚受を示す側面図である。
=棚受=
複数の棚受5、6は、棚板3が載置される。複数の棚受5の棚受51乃至59は夫々、複数の棚受6の棚受61乃至69と対となって棚板3が略水平となるように棚板3を支持する。尚、複数の棚受5の棚受51乃至59は夫々、複数の棚受6の棚受61乃至69と同様な構成であるので、複数の棚受5についてのみ説明し、複数の棚受6についてはその説明を省略する。
=突部=
水皿402(図4)を取り出すために、水皿402における開口21Aに近い側(-Y)の端部を、パッキン210の一部2Dよりも垂直方向における高さが高くなるように持ち上げる必要がある。例えば、水皿402に比較的多量の水が貯留されている場合、水皿402の-Y側の端部が急激に持ちあげられたときに、水皿402内の水がこぼれてしまうことがある。尚、パッキン210の一部2Dは、パッキン210における開口21Aの下側の縁に設けられている部分である。
===棚板===
以下、図10及び図11を参照して、本実施形態における棚板について説明する。図10は、本実施形態における棚板が載置されている状態の棚受を示す側面図である。図11は、本実施形態における培養装置と棚板とを示す図である。尚、図11は、棚板の平面図を示している。図11は、更に、図5の垂直方向(Z軸)における棚受51、52の間を通り且つXY平面に平行な断面から-Z方向に向かって見た培養装置100を示している。
===伝熱シート===
以下、図6、図12乃至図17を参照して、本実施形態における伝熱シートについて説明する。図12は、本実施形態における伝熱シートを示す図である。図13は、本実施形態における内箱の側板と伝熱シートとを示す図である。図14は、本実施形態における内箱の側板と伝熱シートとヒータ線とを示す図である。尚、図13及び図14は、側板23における側板13側(外側)の面(+X)に貼付されている伝熱シート9を示している。図15は、本実施形態における棚受と伝熱シートとを示す断面図である。尚、図15は、図14の線分Z1を含み且つXZ平面に平行な断面から+Y方向へ向かって見た側板23等を示している。図16は、本実施形態におけるストッパと伝熱シートとを示す断面図である。図17は、本実施形態における伝熱シートが窪みに押し込まれた状態のストッパと伝熱シートとを示す断面図である。尚、図16及び図17は、図14の線分Z2を含み且つXZ平面に平行な断面から+Y方向へ向かって見た側板23等を示している。図16は、窪み519に押し込まれていない状態の伝熱シート9を示している。図17は、窪み519に押し込まれた状態の伝熱シート9を示している。
=伝熱シートの構成=
伝熱シート9、9A、9Bは、ヒータ線900から放散される熱を内箱20に伝える。伝熱シート9は、側板23における側板13側(外側)の面(+X)に貼付される。伝熱シート9Aは、側板22における側板12側(外側)の面(-X)に貼付される。伝熱シート9Bは、天板26における天板16側(外側)の面(-X)に貼付される。尚、伝熱シート9、9Aの構成は同様であるので、伝熱シート9の構成についてのみ説明し、伝熱シート9Aの構成については、その説明を省略する。
=伝熱シートの側板への貼付=
伝熱シート9の台紙90を剥離し、接着剤が塗布されている面を側板23の外側の面に貼付される(図13)。
===棚板の出し入れ===
以下、図2、図10及び図18を参照して、本実施形態における棚板の出し入れについて説明する。図18は、本実施形態における棚板と培養装置とを示す側面図である。尚、説明の便宜上、培養装置100における側板22、12は省略されている。
=棚板が入れられる場合=
外扉11及び内扉21が開かれて、開口21Aを介して内箱20内に棚板3を入れることが可能な状態となる。
=棚板が取り出される場合=
外扉11及び内扉21が開かれて、開口21Aを介して内箱20内の棚板3を取り出すことが可能な状態となる。
5、6 複数の棚受
9、9A、9B 伝熱シート
10 外箱
12、13、22、23 側板
15、25、31 底板
20 内箱
51乃至59、61乃至69、501、502 棚受
100 培養装置
91A、91B 補強孔
900 ヒータ線
911乃至917 切れ込み
918、919 抜出孔
Claims (4)
- 外箱と、前記外箱の内側に配置される金属板からなる内箱と、前記内箱の外側に配置されるヒータと、前記外箱及び前記内箱の前面に形成される開口を開閉する扉と、を有し、前記内箱の内部で被培養体を培養する培養装置において、
前記内箱の両側板にプレス加工で形成され、前記被培養体が載置される棚板の底面の両側部が載置される棚受と、
前記内箱の少なくとも一方の側板の外側の面に貼付され、前記ヒータの熱を前記内箱に伝える伝熱シートと、
を備え、
前記伝熱シートは、前記内箱の側板の外側の面に貼付された後、前記棚受が形成されたことにより前記内箱の側板の外側に形成される第1窪みにも貼付されるように、前記伝熱シートを折り曲げるための第1切れ込みを断続的に複数有する
ことを特徴とする培養装置。 - 前記伝熱シートは、前記内箱の側板の外側の前記第1窪みを含む面に貼付された際に生じる気体を抜き出すための抜出孔を有する
ことを特徴とする請求項1に記載の培養装置。 - 前記棚受は、長尺形状を呈し、
前記第1切れ込みは、前記棚受の長手方向に沿って切り込んで形成される
ことを特徴とする請求項1又は2に記載の培養装置。 - 前記内箱の側板は、前記第1窪みをプレス加工で更に窪ませて、前記棚受に載置されている前記棚板の垂直方向の移動を制限するためのストッパが更に形成され、
前記抜出孔は、前記ストッパが形成されたことにより前記第1窪みの内部に形成される第2窪みと対向する位置に設けられ、
前記伝熱シートは、前記内箱の側板の外側の面に貼付された後、前記第2窪みにも貼付されるように、前記伝熱シートを折り曲げるための第2切れ込みを更に有する
ことを特徴とする請求項1乃至3の何れかに記載の培養装置。
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JP2015541648A JP5944063B2 (ja) | 2013-10-11 | 2014-10-10 | 培養装置 |
CN201480050571.4A CN105555944B (zh) | 2013-10-11 | 2014-10-10 | 培养装置 |
EP14852396.2A EP3031901B1 (en) | 2013-10-11 | 2014-10-10 | Culture apparatus |
US15/065,588 US10023834B2 (en) | 2013-10-11 | 2016-03-09 | Culture apparatus |
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JP2004222731A (ja) * | 2004-03-30 | 2004-08-12 | Sanyo Electric Co Ltd | 培養装置 |
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JP5908240B2 (ja) * | 2011-09-22 | 2016-04-26 | パナソニックヘルスケアホールディングス株式会社 | 培養装置 |
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JP7150131B2 (ja) | 2019-02-18 | 2022-10-07 | 株式会社日立ハイテク | 培養容器ラック及び分析装置 |
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EP3031901A1 (en) | 2016-06-15 |
EP3031901B1 (en) | 2017-09-27 |
US10023834B2 (en) | 2018-07-17 |
US20160186122A1 (en) | 2016-06-30 |
CN105555944B (zh) | 2017-03-15 |
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