WO2014097850A1

WO2014097850A1 - Method for producing fermented beverage

Info

Publication number: WO2014097850A1
Application number: PCT/JP2013/082080
Authority: WO
Inventors: 哲平黒川; 巧弥大橋; 耕平渡邊
Original assignee: アサヒビール株式会社
Priority date: 2012-12-19
Filing date: 2013-11-28
Publication date: 2014-06-26
Also published as: JP6208939B2; JP2014117258A

Abstract

The present invention addresses the problem of providing a method for producing a fermented beverage by highly efficiently fermenting a wheat wort having a higher original wheat wort extract concentration than those employed in the conventional high-concentration brewing methods. A means for solving the problem is a method for producing a fermented beverage, comprising the steps of: adding beer yeast to a wheat wort having an original wheat wort extract concentration of 25 to 32 wt%; and fermenting the wheat wort, to which the beer yeast has been added, while aerating the wheat wort for 24 hours or longer.

Description

Method for producing fermented beverage

The present invention relates to a method for producing a fermented beverage, and more particularly to a method for producing a fermented beverage using high-concentration wort.

Fermented beverages refer to beverages obtained by fermenting malt and auxiliary materials, or wort produced by saccharifying only auxiliary materials using beer yeast. Fermented beverages include beer, beverages that have the same taste and aroma as beer, and specific examples include happoshu, sparkling liqueurs, and other brewed liquors.

High concentration brewing of beer has been performed for the purpose of improving production efficiency and reducing environmental impact. High concentration brewing is fermented with wort of higher than normal wort extract to obtain a high alcohol concentration fermented liquid, and this fermented liquid is diluted with water to obtain a beer with a desired alcohol concentration. It is a brewing method.

High-concentration brewing has the advantage of increasing the production efficiency of manufacturing equipment such as fermentation and storage tanks and reducing energy costs. On the other hand, there is a problem that the fermentation time becomes longer because a large amount of the raw wort extract is fermented, and the flavor is different from that produced by a normal method.

That is, normal beer fermentation is often performed at a raw wort extract concentration of about 12 to 15%. Fermentation at a high concentration exceeding 20% results in delayed fermentation, poor uptake of extract / amino acid, death of yeast, and the like, resulting in beer having a poor flavor balance such as remaining sweet taste or strong yeast odor.

Patent Document 1 describes a method for producing beer in which an enzyme is allowed to act during fermentation when performing high-concentration brewing. However, this method, like sake, is a brewing method in which saccharification and fermentation proceed simultaneously, and it requires a long fermentation period of 20 days or more.

Patent Document 2 describes a method for improving the maltose-assimilating fermentability of yeast by gene recombination, fermenting high-concentration wort using the yeast, and obtaining high-concentration beer. However, gene recombination requires advanced techniques and equipment, and complicated operations, and this method is not practical.

In Patent Document 3, in the production of a fermented malt beverage that is fermented by adding yeast to wort, fermentation of wort is promoted by aeration, and the fermentation ability is poor even if the aeration time is too short or too long. It is described that there is a proper constant ventilation time. Based on this knowledge, a method for producing a fermented malt beverage is described in which wort having an original wort extract concentration of 22% is fermented while being aerated for 8 to 14 hours.

JP 2002-253197 A JP-A-6-245750 JP 2003-102458 A

The present invention solves the above-mentioned conventional problems, and an object of the present invention is to provide a method for producing a fermented beverage that efficiently ferments wort having an original wort extract concentration higher than that of conventional high-concentration brewing. It is in.

The present invention includes a step of adding brewer's yeast to wort having an original wort extract concentration of 25 to 32% by weight; ;
A method for producing a fermented beverage is provided.

In one certain form, fermentation temperature is 12 degreeC or more in the said method.

In one certain form, the addition amount of beer yeast is 30 * 10 < ⁶ > cells / ml or more.

In one embodiment, the brewer's yeast is the bottom brewer's yeast.

In one embodiment, fermentation is performed until the residual concentration of the fermentable extract is 1% or less.

In one embodiment, fermentation is performed until the alcohol concentration reaches 12% or more.

The present invention also provides a fermented beverage having a fermentable extract residual concentration of 1% or less and an alcohol concentration of 12% or more.

According to the method of the present invention, there is provided a method for producing a fermented beverage from which a fermented beverage having a good flavor balance can be obtained using wort having a higher concentration of the original wort extract than conventional high-concentration brewing. As a result, in the production of fermented beverages, production efficiency is improved, the environmental burden is reduced, and in addition, a fermented beverage having a high alcohol concentration of 12% or more and 15% or more is provided.

(A) is a graph showing the relationship between the value of extract difference and aeration time, and (b) is a graph showing the relationship between alcohol concentration and aeration time.

First, wort is manufactured. Here, wort refers to a sugar solution produced by saccharification of only malt and auxiliary materials, or auxiliary materials such as soybean protein degradation products. For example, wort is produced by heating crushed malt, auxiliary materials such as barley, and warm water to produce saccharose. The wort used for fermentation is high-concentration wort. The high-concentration wort preferably has a raw wort extract concentration of 25% to 35% by weight, more preferably 27% to 32% by weight, and still more preferably 29 to 31% by weight. When the concentration of the original wort extract is 25% by weight or more, the above-mentioned advantages due to high concentration brewing can be easily obtained, and when it exceeds 35% by weight, the viscosity of the wort becomes high and filtration becomes difficult.

As a method for preparing high-concentration wort, (1) changing the ratio of raw material malt and the like in the preparation step and the preparation water (preparation composition), (2) evaporating and condensing the water by boiling, (3) Examples include changing the amount of warm water added for the preparation of the extract after boiling, and (4) using liquid sugar or malt extra. The saccharification temperature of raw materials such as malt may be controlled by the infusion method or the decoration method.

The wort is then fermented. The wort fermentation is performed under aerated condition for at least a predetermined time. Specifically, wort is put into a fermenter, beer yeast is added to the wort, and air is blown into it. You may add beer yeast before putting wort in a fermenter. The amount of air blown into the wort is appropriately adjusted according to the desired flavor quality, the kind of yeast, etc., but is generally 0 to 15 ppm in dissolved oxygen.

Aeration is preferably performed for 24 hours or more. By doing so, the amount of the appearance extract remaining in the fermentation liquid is reduced, and a refreshing flavor with less sweetness is realized. There is no upper limit to the aeration time, and it may be performed over the fermentation process. However, even if aeration is performed over 50 hours, the effect of promoting fermentation is reduced. The aeration time is preferably 24 to 72 hours.

It is preferable to start aeration immediately after adding brewer's yeast. By doing so, it is possible to maintain the aerobic metabolism of the yeast and promote the growth of the yeast and the change of the cell membrane composition. Aeration may be performed intermittently as long as dissolved oxygen does not disappear.

Yeast added to wort is beer yeast. As the brewer's yeast, the upper fermented yeast generally has higher alcohol resistance, but the lower fermented yeast may be used in the present invention.

The amount of yeast added to the wort is 30 × 10 ⁶ (cells / ml) or more, preferably 30 to 50 × 10 ⁶ per ml of wort. If the amount of yeast to be added is less than 30 × 10 ⁶ cells / ml, the raw wort extract is not sufficiently consumed during the fermentation process, and sweetness remains. When the amount of yeast exceeds 80 × 10 ⁶ cells / ml, the yeast odor becomes too strong.

The fermentation temperature is 12 ° C or higher, preferably 14-17 ° C, more preferably 15 ° C. If the fermentation temperature is less than 12 ° C., the raw wort extract is not sufficiently consumed and the sweetness remains. In the bottom fermentation yeast, if the fermentation temperature exceeds 17 ° C., the yeast tends to be killed, and the fermentation may not proceed normally.

The fermentation time varies depending on the type of yeast used, but is 300 hours to 480 hours, preferably 330 hours to 400 hours. When the fermentation time is less than 300 hours, the fermentation is not completed sufficiently, and when it exceeds 480 hours, the yeast odor becomes too strong.

Further, as a ripening step, the obtained fermentation broth is aged at about 15 ° C. in a storage tank, and stored and stabilized at about −1 to 0 ° C. Subsequently, as a filtration step, yeast, protein, and the like are removed by filtering the fermented solution after ripening, and a desired fermented solution having a high alcohol concentration is obtained. The alcohol concentration of the obtained fermentation broth is 12 V / V% or more, preferably 15 V / V% or more.

The fermented liquid after aging is processed in the production of ordinary fermented beverages, for example, filtration, adjustment of final concentration by degassed water, injection of carbon dioxide gas, addition of flavor, seasoning or coloring agent, pasteurization ( A fermented beverage is prepared by appropriately performing pasting), filling (packaging) into containers (for example, barrels, cans, cans), and labeling containers.

The present invention will be described more specifically with reference to the following examples, but the present invention is not limited thereto.

Example 1
The harvested barley was immersed in water to germinate properly and then dried with hot air to produce malt. The malt was crushed by a conventional method. Next, 25 kg of crushed malt and 62.5 L of warm water were added to the charging tank and mixed to prepare a miche. Miche was prepared by holding at 50 ° C for 30 minutes. Then, the starch was gradually saccharified using an enzyme derived from malt by gradually raising the temperature of the mash and holding it at a predetermined temperature for a certain period of time. The saccharification treatment was performed by holding at 64.5 ° C. for 10 minutes and at 70 ° C. for 10 minutes. After maintaining the saccharification treatment at 78 ° C. for 5 minutes, about 100 L of transparent wort was obtained by filtering the miche in a wort filtration tank.

The obtained wort was transferred to a boiling kettle. 17.5 kg of malt extract, 20 kg of sugar solution and hops were added to the wort. Subsequently, wort boiling was performed at 100 ° C. for 70 minutes by heating the boiling pot. The original wort extract concentration of the wort was adjusted to 30% by weight. The wort was transferred to a settling tank, and clear wort was obtained by removing the hop cake that had sunk in the lower layer.

The obtained wort was cooled to 12 ° C. The cooled wort was transferred to a fermentation tank, and 30 × 10 ⁶ muddy yeasts (lower beer yeast) were added to the fermentation tank per 1 ml of wort. Fermentation was performed at 15 ° C. while air was blown into the fermentation tank at a flow rate of 1.0 NL / min.

Aeration was started immediately after the addition of brewer's yeast, and the wort was changed for 0 hours, 12 hours, 24 hours, 36 hours, 48 hours and 72 hours, respectively. Thereafter, the fermentation was continued and finished when the fermentation time reached about 360 hours. As a result, five types of fermentation broths having different aeration times were obtained.

The residual concentration of fermentable extract (extract difference) of the obtained fermentation broth was measured. The fermentable extract residual concentration is based on the concentration of an extract (fermentable extract) that can be assimilated by yeast contained in wort. It is a value representing the concentration of.

FIG. 1 (a) is a graph showing the relationship between the extract difference value of the fermentation broth and the aeration time. From this graph, it can be seen that the extract difference decreases to 1% or less when the aeration time is 24 hours or more.

FIG. 1 (b) is a graph showing the relationship between the alcohol concentration value of the fermentation broth and the aeration time. From this graph, it can be seen that the alcohol concentration increases to 15% or more when the aeration time is 24 hours or more.

These fermentation broths were subsequently aged in a storage tank and stored and stabilized at about -1 to 0 ° C. Degassed water was added so that the obtained fermented liquor was 5 V / V% alcohol to produce beer.

Among the obtained beers, those with aeration time of 12 hours, 24 hours and 72 hours were subjected to sensory evaluation. At that time, several evaluators sampled beer and expressed the impression of taste. The percentage of people who pointed out the taste based on the number of evaluators (number of indications) was taken as the indication rate. The results are shown in Tables 1 to 3.

[Table 1]
Aeration time 12, number of evaluators 11

[Table 2]
Ventilation time 24, 7 evaluators

[Table 3]
Ventilation time 72, number of evaluators 5

Sensory evaluation showed that the sweetness of beer decreased when the aeration time was 24 hours or longer.

Next, of the obtained beer, those that were not vented and those that were vented for 48 hours were subjected to sensory evaluation. At that time, several evaluators sampled beer and expressed the impression of taste. The number of people who pointed out the taste based on the number of evaluators was recorded. The results are shown in Table 4.

[Table 4]
Number of evaluators 6

*: 4-Vinylguaiacol

Furthermore, the same evaluator was asked which is stronger for sweetness and which is better for flavor balance. The results are shown in Table 5.

[Table 5]
Number of evaluators 6

Table 4 and Table 5 show that, when aeration is performed for 48 hours, beer with less sweetness and good flavor balance can be produced.

Example 2 and Comparative Example 1
In the same manner as in Example 1, wort having an original wort extract concentration of 30% by weight was prepared. The wort was cooled to 12 ° C., the cooled wort was transferred to a fermentation tank, and 40 × 10 ⁶ muddy yeasts (lower beer yeast) were added to the fermentation tank per 1 ml of wort. Fermentation was performed at 15 ° C. while air was blown into the fermentation tank at a flow rate of 1.0 NL / min.

Aeration started immediately after adding brewer's yeast and ended after 36 hours. Thereafter, the fermentation was continued and finished when the fermentation time reached about 360 hours. Subsequently, it was aged in a storage tank, stored under conditions of about -1 to 0 ° C and stabilized to obtain a fermentation broth. The fermentable extract residual concentration of the obtained fermentation broth was 0.60%, and the alcohol concentration was 15.49 V / V%.

As a comparative example, a fermentation broth was obtained in the same manner as in Example 3 except that 20 × 10 ⁶ yeasts were added per 1 ml of wort. The fermented extract residual concentration of the obtained fermentation broth was 6.69%, and the alcohol concentration was 12.84 V / V%.

From the above results, it was shown that when the amount of yeast added to the wort is small, the fermentable extract is not consumed sufficiently and the amount of alcohol produced is also reduced.

Example 3 and Comparative Example 2
In the same manner as in Example 1, wort having an original wort extract concentration of 30% by weight was prepared. The wort was cooled to 12 ° C., the cooled wort was transferred to a fermentation tank, and 40 × 10 ⁶ muddy yeasts (lower beer yeast) were added to the fermentation tank per 1 ml of wort. Fermentation was performed at 15 ° C. while air was blown into the fermentation tank at a flow rate of 1.0 NL / min.

Aeration started immediately after the addition of brewer's yeast and ended after 24 hours. Thereafter, the fermentation was continued and finished when the fermentation time reached about 360 hours. Subsequently, it was aged in a storage tank, stored under conditions of about -1 to 0 ° C and stabilized to obtain a fermentation broth. The fermentable extract residual concentration of the obtained fermentation broth was 0.99%, and the alcohol concentration was 15.14 V / V%.

As a comparative example, a fermentation broth was obtained in the same manner as in Example 4 except that fermentation was performed at 10 ° C. The fermentable extract residual density | concentration of the obtained fermented liquor was 4.69%, and alcohol concentration was 12.79 V / V%.

From the above results, it was shown that when the fermentation temperature is low, the fermentable extract is not consumed sufficiently and the amount of alcohol produced is also reduced.

Claims

Adding brewer's yeast to wort having an original wort extract concentration of 25-32% by weight;
The manufacturing method of fermented drinks including this.
The method for producing a fermented beverage according to claim 1, wherein the fermentation temperature is 12 ° C or higher.
The manufacturing method of the fermented drinks of Claim 1 or 2 whose addition amount of beer yeast is 30 * 10 < 6 > cells / ml or more.
The method for producing a fermented beverage according to any one of claims 1 to 3, wherein the brewer's yeast is lower surface brewer's yeast.
The method for producing a fermented beverage according to any one of claims 1 to 4, wherein the fermentation is performed until the residual concentration of the fermentable extract is 1% or less.
The method for producing a fermented beverage according to any one of claims 1 to 5, wherein the fermentation is performed until the alcohol concentration reaches 12% or more.
Fermented beverage having a fermentable extract residual concentration of 1% or less and an alcohol concentration of 12% or more.