WO2024053297A1 - Beer-taste alcoholic beverage - Google Patents

Beer-taste alcoholic beverage Download PDF

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Publication number
WO2024053297A1
WO2024053297A1 PCT/JP2023/028279 JP2023028279W WO2024053297A1 WO 2024053297 A1 WO2024053297 A1 WO 2024053297A1 JP 2023028279 W JP2023028279 W JP 2023028279W WO 2024053297 A1 WO2024053297 A1 WO 2024053297A1
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WIPO (PCT)
Prior art keywords
beer
taste
alcoholic beverage
wort
taste alcoholic
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PCT/JP2023/028279
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French (fr)
Japanese (ja)
Inventor
一志 青野
真維 寺野
Original Assignee
アサヒグループホールディングス株式会社
アサヒビール株式会社
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Application filed by アサヒグループホールディングス株式会社, アサヒビール株式会社 filed Critical アサヒグループホールディングス株式会社
Publication of WO2024053297A1 publication Critical patent/WO2024053297A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C12/00Processes specially adapted for making special kinds of beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • C12C5/02Additives for beer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/04Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs

Definitions

  • the present invention relates to a beer-taste alcoholic beverage, and particularly to a beer-taste low alcoholic beverage with a low alcohol concentration.
  • Beer is a beverage that has an alcohol concentration of 4.5 to 5% by volume and contains carbon dioxide gas. Beer achieves palatability by, for example, giving a feeling of satisfaction when drinking beer, such as the richness and body feeling, and giving a feeling of refreshment due to the stimulation of carbonic acid.
  • Patent Document 1 describes a low-alcohol fermented malt beverage in which the alcohol concentration is lowered by increasing the proportion of non-fermentable carbohydrates in the wort.
  • a low-alcohol fermented malt beverage is described, which is characterized by the addition of acid.
  • the low-alcohol fermented malt beverage of Patent Document 1 does not have prominent sweetness or other unnatural flavors, and has a well-balanced taste and flavor comparable to that of ordinary beers (Summary) .
  • the sweetness of non-fermentable carbohydrates imparts a rich taste and body feeling similar to that of beer, while the sourness of organic acids does not overpower the sweetness, resulting in a well-balanced flavor. It is taken.
  • Patent Document 2 describes a beer-taste beverage characterized by containing 5 ppm or more of hydroxy acid esters.
  • the beer-taste beverage of Patent Document 2 has enhanced carbonic acid stimulation without increasing carbon dioxide gas pressure (summary). It is speculated that the bitterness of hydroxy acid esters is felt at the same time as the stimulation by carbonic acid, giving the illusion of carbonic acid stimulation, and as a result, the stimulation of carbonic acid is enhanced.
  • Beer-taste alcoholic beverages have unique features such as low calorie content, so when compared to regular beer, they lack a sense of satisfaction or refreshment. Therefore, it is desired to improve palatability by enhancing richness, body feel, carbonic acid stimulation, etc.
  • beer-taste alcoholic beverages with high carbon dioxide gas pressure require the use of containers with high pressure resistance in order to be distributed on the market, which is costly. Furthermore, if the increased carbon dioxide pressure exceeds the allowable gas pressure of the filling equipment, new equipment will need to be installed. Furthermore, when taking measures to enhance carbonic acid stimulation, it is preferable that the flavor of the beer-taste alcoholic beverage is not affected.
  • the present invention solves the above-mentioned problems, and its purpose is to provide a beer-taste alcoholic beverage with enhanced carbonic acid stimulation without increasing carbon dioxide gas pressure and without affecting flavor. It is in.
  • the present invention includes the following aspects.
  • Beer taste having a viscosity of 2.0 mPa ⁇ s or more, preferably 2.0 to 5.0 mPa ⁇ s, more preferably 2.1 to 3.5 mPa ⁇ s, even more preferably 2.3 to 3.2 mPa ⁇ s alcoholic drinks.
  • a method for producing a beer-taste alcoholic beverage comprising:
  • a beer-taste alcoholic beverage with enhanced carbonic acid stimulation is provided without increasing carbon dioxide gas pressure and without affecting flavor.
  • Beer-taste beverage refers to a carbonated beverage that has a flavor and carbonic acid stimulation reminiscent of beer.
  • “Beer” refers to a fermented malt beverage obtained by fermenting malt, hops, water, etc. with yeast as raw materials.
  • beer-taste drinks those containing alcohol are referred to as “beer-taste alcoholic drinks.”
  • alcohol means ethanol.
  • beer-taste drinks those that contain malt as a starchy raw material and are produced through a fermentation process are called fermented malt drinks.
  • Beer-taste alcoholic beverages include beer, low-malt beer, liqueurs obtained by mixing beer or low-malt beer with an alcohol-containing distillate, and the like.
  • the beer-taste alcoholic beverage of the present invention has a viscosity of 2.0 mPa ⁇ s or more. Viscosity is an indicator of the degree of viscosity of a substance.
  • the viscosity refers to a value measured using a viscometer for a beer-taste alcoholic beverage at a liquid temperature of 8.5°C.
  • a specific example of the viscometer is a tuning fork vibrating viscometer.
  • the viscosity of the beer-taste alcoholic beverage is preferably 2.0 to 5.0 mPa ⁇ s, more preferably 2.1 to 3.5 mPa ⁇ s, and still more preferably 2.3 to 3.2 mPa ⁇ s.
  • the viscosity of a beer-taste alcoholic beverage refers to the viscosity of the final product, the beverage.
  • the viscosity of the beer-taste alcoholic beverage can be adjusted in any step included in its production method. Examples of methods for adjusting the viscosity of the beer-taste alcoholic beverage include methods such as changing the type and amount of raw materials used, or adding a thickener as appropriate.
  • a thickener is a substance that increases the viscosity when included in a beer-taste alcoholic beverage.
  • Thickeners are not particularly limited as long as they are substances that can be used in foods and drinks and do not affect flavor in normal usage amounts, but include, for example, pectin, ⁇ -carrageenan, gellan gum, xanthan gum, and locust bean gum. , agar, and other thickening polysaccharides. These may be used alone or in combination of two or more. Among these, pectin, xanthan gum and agar are preferred from the viewpoint that the flavor of the thickener itself is more suitable for the flavor of beer.
  • Pectin is a polysaccharide whose main component is D-galacturonic acid
  • xanthan gum is a polysaccharide produced by fermentation by bacteria of the genus Xanthomonas
  • agar is a polysaccharide whose main components are agarose and agaropectin. It is a polysaccharide.
  • the type and content of the thickener are adjusted so that the viscosity of the beer-taste alcoholic beverage falls within the aforementioned predetermined range.
  • the content of the thickener in the beer-taste alcoholic beverage is 0.05 to 1 w/v%, preferably 0.1 to 0.9 w/v%, more preferably 0.2 to 0.8 w/v %.
  • the beer-taste alcoholic beverage of the present invention may have an alcohol concentration comparable to that of beer.
  • the beer-taste alcoholic beverage of the present invention is 6% by volume (v/v) or less, preferably 1 to 6% by volume, more preferably more than 1% by volume to 6% by volume, even more preferably 1% by volume. .5 to 5.5% by volume.
  • Methods for measuring alcohol concentration (i.e., ethanol concentration) in beverages are widely known to those skilled in the art, and can be measured, for example, by the method described in "National Tax Agency Specified Analysis Method.”
  • the beer-taste alcoholic beverage of the present invention is a beer-taste low-alcohol beverage with a lower alcohol concentration than normal beer.
  • the beer-taste low alcoholic beverage has an alcohol concentration of 4% by volume or less, preferably 1 to 4% by volume, more preferably 2 to 3.9% by volume, and even more preferably 3 to 3.8% by volume.
  • Beer-taste low-alcohol beverages have a problem in that they are less sensitive to carbonation than regular beer, and do not provide an adequate refreshing feeling when drinking.
  • the carbonic acid contained in beer volatilizes and irritates the mouth and throat, but when the alcohol concentration is low, the amount of alcohol that volatilizes with the carbonic acid decreases, and the irritating effect of alcohol decreases, resulting in a decrease in the strength of the carbonic acid irritation. It is thought that then.
  • Examples of methods for adjusting the alcohol concentration of the beer-taste alcoholic beverage include methods such as lowering the final fermentation degree of the wort fermentation liquid, or reducing the amount of alcohol added.
  • the method for producing a beer-taste alcoholic beverage of the present invention will be specifically explained below.
  • the beer-taste alcoholic beverage of the present invention can be produced, for example, by fermenting wort with beer yeast.
  • ⁇ wort> Wort is a liquid containing water and a saccharified solution of starchy raw materials. Wort is produced, for example, as follows.
  • auxiliary raw materials such as barley, and hot water are added to a brewing tank and mixed to prepare maiche.
  • the resulting beer-taste alcoholic beverage becomes a fermented malt beverage.
  • the main raw material is either not used or grains other than malt are used as the main raw material.
  • Preparation of maiche can be carried out by a conventional method, for example, first, the protein derived from the raw material is decomposed into amino acids etc. by holding it at 35 to 60°C for 20 to 90 minutes, and then the process proceeds to the saccharification step. At that time, in addition to the main raw materials and auxiliary raw materials, saccharifying enzymes and flavor components such as spices, herbs, and fragrances are added as necessary.
  • the temperature of the mache is gradually raised and held at a predetermined temperature for a certain period of time to saccharify the starch using malt-derived enzymes and enzymes added to the mache.
  • the temperature and time during the saccharification treatment should be determined by taking into account the type of enzyme used, the amount of mache, the quality of the target wort beer yeast fermentation liquid (hereinafter sometimes simply referred to as "wort fermentation liquid"), etc.
  • the temperature can be determined as appropriate, for example, by holding the temperature at 60 to 72°C for 30 to 90 minutes.
  • the mixture is maintained at 76 to 78°C for about 10 minutes, and then the mache is filtered to obtain a transparent sugar solution.
  • the raw material subjected to saccharification contains malt.
  • the content of malt in the raw material to be subjected to saccharification is not particularly limited, but is 25% or more, preferably 50% or more, and more preferably 67% or more.
  • the raw material subjected to saccharification may have a malt usage ratio of 100%.
  • the ratio of malt (weight (w/w) %) to the raw materials to be subjected to saccharification is referred to as the malt usage ratio.
  • the higher the malt usage ratio the stronger the malt-derived flavor, richness, and drinkability of the resulting wort fermentation liquid will be.
  • the raw material subjected to saccharification preferably contains oats.
  • the content of oats in the raw material to be subjected to saccharification is not particularly limited, but is 15% or more, preferably 20 to 80%, more preferably 40 to 60%.
  • the oats used are preferably those that have been subjected to a malting process to produce malt through germination.
  • auxiliary raw materials refer to raw materials other than malt and hops.
  • auxiliary raw materials include starch raw materials such as barley, wheat, cornstarch, corn grits, rice, and soybean, and carbohydrate raw materials such as liquid sugar and sugar.
  • starch raw materials such as barley, wheat, cornstarch, corn grits, rice, and soybean
  • carbohydrate raw materials such as liquid sugar and sugar.
  • liquid sugar is produced by decomposing and saccharifying starch with an acid or a saccharifying enzyme, and mainly contains glucose, maltose, maltotriose, and the like.
  • spices, herbs, fruits, etc. used for the purpose of imparting or improving flavor are also included in the auxiliary raw materials.
  • Saccharifying enzyme refers to an enzyme that decomposes starch to produce sugar.
  • Transglucosidase is preferably used as the saccharifying enzyme. When transglucosidase is used, the amount of non-assimilable sugars contained in the wort can be changed, making it easy to adjust the final fermentation degree of the wort fermentation liquid and the extract concentration of the wort fermentation liquid. .
  • the saccharifying enzyme for example, ⁇ -amylase, glucoamylase, prunalase, etc. may be used in combination with transglucosidase.
  • transglucosidase is not particularly limited as long as it has catalytic activity for transglycosylation reactions, and transglucosidases derived from various organisms can be used.
  • transglucosidases derived from various organisms can be used.
  • any commercially available transglucosidase may be used, or a combination of these may be used.
  • transglucosidase to mashed potatoes is not particularly limited as long as the enzymatic reaction by the added transglucosidase is sufficiently carried out by the end of the preparation process.
  • transglucosidase may be added together with fermentation raw materials such as malt at the time of preparing mache, or may be added during the saccharification reaction.
  • transglucosidase in order to allow the enzymatic reaction by transglucosidase to proceed sufficiently, transglucosidase is preferably added at the time of preparing mache or at an early stage of the preparation process, and more preferably added at the time of preparing mache.
  • the wort boiling operation may be performed according to the method and conditions normally used when producing beer. For example, a pH-adjusted sugar solution is transferred to a boiling pot and boiled. Hops are added from the time the sugar solution starts boiling to the time it is left in the whirlpool. As the hop, a hop extract or a component extracted from hops may be used.
  • the sugar solution is then transferred to a settling tank called a whirlpool, and after removing hop residue and coagulated proteins produced by boiling, it is cooled to an appropriate temperature using a plate cooler.
  • a settling tank called a whirlpool
  • a plate cooler By the above-mentioned operation up to wort boiling, wort as referred to in the present invention is obtained.
  • transglucosidase is used as the saccharifying enzyme
  • the resulting wort is called transglucosidase-treated wort.
  • Non-assimilable carbohydrates refer to carbohydrates that are not used by brewer's yeast as a nutrient source for alcohol fermentation, etc.
  • the brewer's yeast used in the present invention assimilates glucose, fructose, maltose, and maltotriose. Therefore, carbohydrates other than these are collectively called non-assimilated carbohydrates.
  • Specific examples of non-assimilable carbohydrates contained in the transglucosidase-treated wort include isomaltose, isomaltotriose, panose, oligosaccharides of 4 or more sugars, and non-assimilable polysaccharides. The non-assimilable carbohydrates remain even after the fermentation process and alcohol removal process described below.
  • the amount of transglucosidase used when producing Magnoliahe is adjusted as appropriate, taking into account the type and reaction conditions, so that the final fermentation degree of the wort fermentation liquid or the extract concentration of the wort fermentation liquid is within the above range. be done.
  • the amount of transglucosidase used is 0.05 to 20 g/kg, preferably 0.1 to 7 g/kg, more preferably 0.25 to 5 g/kg, based on the solid content of the mache.
  • the wort extract concentration of the wort is preferably 7-20°P, more preferably 8-15°P.
  • ⁇ wort fermentation liquid> The obtained wort is fermented with brewer's yeast. Fermentation of wort may be carried out according to conventional methods. For example, cooled wort is inoculated with brewer's yeast, transferred to a fermentation tank, and alcoholic fermentation is performed.
  • the appearance and final fermentation degree of the wort fermentation liquid is 95% or less, for example, 30 to 95%. Appearance of the fermented wort If the final fermentation degree is less than 30%, the complex flavor may be weak and the sweetness may not be sufficiently suppressed, and the sweetness or richness of the resulting beer-taste alcoholic beverage may remain as an aftertaste, resulting in a sharp taste. The sensation and refreshing feeling after drinking become insufficient. If the appearance of the wort fermented liquid exceeds 95%, the resulting beer-taste alcoholic beverage may have insufficient texture or richness, and may be too sour, resulting in a loss of beer-like flavor.
  • the final fermentation degree in appearance of the wort fermented liquid is preferably 85% or less, for example, 35 to 85%, more preferably 35 to 60%, and still more preferably 40 to 50%.
  • the degree of fermentation is an index that indicates how much fermentation has progressed in beer after fermentation.
  • the final fermentation degree refers to the ratio of extract that can be assimilated by brewer's yeast to the original wort extract.
  • the extract that can be assimilated by brewer's yeast refers to the extract contained in the product beer from the original wort extract (i.e., the extract that remains after fermenting all the extracts that can be used by brewer's yeast (referred to as the final extract). ) is deducted.
  • the appearance final fermentation degree refers to the final fermentation degree calculated using the appearance extract as the value of the final extract.
  • extract refers to nonvolatile solid content.
  • extract means, depending on the context, the non-volatile solids themselves, the amount of non-volatile solids, or the concentration of non-volatile solids.
  • the appearance extract concentration refers to the concentration (°P) of nonvolatile solids in a liquid containing alcohol (ethanol).
  • the specific gravity and alcohol concentration of the wort fermentation liquid can be measured by the BCOJ beer analysis method, 8.4.3 and 8.3.6 (both alcoholizer methods).
  • the appearance extract concentration can be calculated using these measured values by the BCOJ beer analysis method and the 8.5 extract relationship calculation method.
  • the appearance and final fermentation degree Vend of the fermented wort liquid can be determined, for example, by the following formula (1).
  • Vend (%) ⁇ (P-Eend)/P ⁇ 100 (1) [Wherein, P is the raw wort extract, and Eend is the final appearance extract. ]
  • the raw wort extract P is calculated by theoretically back-calculating the value of the wort extract before alcoholic fermentation from the alcohol concentration of the product beer and the value of the extract according to Balling's formula. Specifically, it can be determined by the method shown in Analytica-EBC (9.4) (2007).
  • the appearance final extract Eend is obtained by collecting beer in a flask, adding a large amount of freshly pressed yeast, and fermenting it at 25°C with stirring until the extract value no longer decreases (24 hours). It can be determined by measuring the value of appearance extract in beer.
  • Appearance final extract Eend is calculated from the specific gravity of the final extract including alcohol, so it may show a negative value. As a result, the apparent final fermentation degree may exceed 100%.
  • the final fermentation degree in appearance can be controlled by, for example, adjusting the saccharification conditions, whether or not enzymes are used when saccharifying the raw materials, and the types and amounts of raw materials. For example, by lowering the amount of assimilable carbohydrates contained in mache, the final fermentation degree in appearance can be lowered.
  • the resulting wort fermentation liquid is further matured in a sake storage tank as a maturing step, and then stored and stabilized under low temperature conditions of about 0°C.
  • the aged wort fermented liquid is filtered to remove yeast, proteins, and the like.
  • the wort fermentation liquor has an authentic extract concentration of 3-15°P.
  • the authentic extract concentration of the wort fermentation liquid is preferably 5-10°P, more preferably 7-9°P.
  • the authentic extract concentration refers to the mass concentration (°P) of nonvolatile solids contained in the beverage.
  • the authentic extract concentration can be measured by BCOJ beer analysis method, 8.4.3 (alcolyzer method).
  • a pH adjuster is added to the wort fermentation liquid to lower the pH.
  • the bacteriostatic properties of the beer-taste low-alcohol beverage may be insufficient and storage stability may be reduced.
  • Sufficiently lowering the pH provides the bacteriostatic properties and storage stability necessary for marketing beer-taste low-alcohol beverages.
  • the specific upper limit of pH is less than 4.2, preferably less than 4.1, more preferably less than 4.0.
  • the specific lower limit of pH is 3.0 or more, preferably 3.3 or more, more preferably 3.6 or more.
  • the type of pH adjuster is not limited. Not limited to food additives, for example, acids and salts thereof that can be used in beverages and foods, their manufacturing processes, and beer raw materials having pH lowering ability can be used as pH adjusters. Examples of beer raw materials having the ability to lower pH include sour malt, dark malt, and the like.
  • Preferred pH adjusting agents are phytic acid, citric acid, lactic acid, lactic acid bacteria, phosphoric acid, malic acid, sulfite anhydride, tartaric acid, gluconic acid, acetic acid, succinic acid, adipic acid, itaconic acid, fumaric acid, and combinations thereof.
  • More preferred pH adjusting agents are phytic acid, lactic acid, lactic acid bacteria, phosphoric acid, malic acid, sulfite anhydride, tartaric acid, and combinations thereof. Considering its influence on the flavor of beer-taste beverages, phytic acid, which has less sourness, is the most preferred among these.
  • carbon dioxide gas is added to the wort fermentation liquid through a carbonation process. This enhances the carbonation stimulation of the beer-taste alcoholic beverage.
  • the amount of carbon dioxide gas added is adjusted so that the foaming properties are similar to those of beer.
  • the gas pressure of the carbon dioxide gas in the beer-taste alcoholic beverage is adjusted within a range that does not exceed the pressure resistance of the container to be filled or the allowable gas pressure of the filling equipment.
  • the carbon dioxide pressure is, for example, 0.25 MPa or less, preferably 0.20 to 0.24 MPa, more preferably 0.22 to 0.24 MPa.
  • the carbon dioxide pressure of a carbon dioxide solution as used herein means a value measured at a liquid temperature of 20° C. according to a conventional method.
  • the beer-taste alcoholic beverage of the present invention thus obtained is filled into a predetermined container and distributed on the market as a product.
  • the method for packaging the beer-taste alcoholic beverage is not particularly limited, and any method known to those skilled in the art can be used.
  • the beer-taste alcoholic beverage of the present invention is filled into a container and sealed.
  • containers of any form and material may be used. Examples of containers include bottles, cans, barrels, and PET bottles. , bottles or plastic bottles are preferred.
  • ⁇ Comparative example 1> [Manufacture of beer-taste beverages] 400 kg of barley malt, 160 kg of cornstarch, and 1500 liters of hot water were introduced into a preparation tank, and saccharification was performed at 65° C. for 60 minutes. This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 8°C using a plate cooler to obtain wort.
  • Brewer's yeast was added to the obtained wort and fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days. The wort fermentation liquid was clarified by filtration and water was added to adjust the original wort extract to 11.19°P. This was filled into a container as a beer-taste drink.
  • Carbonic acid stimulation intensity was measured by a sensory test. That is, five trained panelists specializing in beer sampled the beer-taste beverages and scored them on a scale of 1 to 10 on a scale of 1 to 10 based on the sensory sensations described below. The average score of all panelists was used as the score for each evaluation item. Note that the liquid temperature of the samples used for evaluation was adjusted to about 4°C. It is judged to be good if the evaluation score is 5 points or more.
  • Carbonated water with adjusted carbon dioxide gas pressure was prepared and used as an evaluation standard for carbonic acid stimulation strength.
  • ⁇ Comparative example 2> [Manufacture of beer-taste beverages] 500 kg of barley malt and 1500 liters of hot water were introduced into a preparation tank, and saccharification was performed at 65° C. for 10 minutes. This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 8°C using a plate cooler to obtain wort.
  • Brewer's yeast was added to the obtained wort and fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days. The wort fermentation liquid was clarified by filtration and water was added to adjust the original wort extract to 9.06°P. This was filled into a container as a beer-taste drink.
  • Examples 1 to 4 [Manufacture of beer-taste beverages] Powdered pectin manufactured by Unitec Foods was prepared as a thickener. The pectin was added and dissolved in the beer-taste beverage obtained in Comparative Example 2 to increase the viscosity to produce a beer-taste beverage, which was filled into containers and measured for characteristics in the same manner as in Comparative Example 1. Table 2 shows the amount of pectin added and the measured properties for each example.
  • Example 5 [Manufacture of beer-taste beverages] 400 kg of barley malt, 160 kg of cornstarch, 1500 liters of hot water, and 3.0 kg of transglucosidase (“Transglucosidase L” (trade name) manufactured by Amano Enzyme Co., Ltd.) were introduced into a preparation tank, and saccharification was carried out at a temperature in the range of 60°C to 76°C. I did it. This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 8°C using a plate cooler to obtain transglucosidase-treated wort.
  • Transglucosidase L (trade name) manufactured by Amano Enzyme Co., Ltd.
  • brewer's yeast was added to the transglucosidase-treated wort, fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days. The fermented wort liquid was clarified by filtration and filled into containers as a beer-taste beverage.
  • Example 6 [Manufacture of beer-taste beverages] 250 kg of oat malt, 250 kg of barley malt, 1500 liters of hot water, and 2.0 kg of transglucosidase ("Transglucosidase L" (trade name) manufactured by Amano Enzyme Co., Ltd.) were introduced into a preparation tank, and the temperature was maintained in the range of 60°C to 76°C. Saccharification was performed using This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 10°C using a plate cooler to obtain transglucosidase-treated wort.
  • Transglucosidase L trade name
  • brewer's yeast was added to the transglucosidase-treated wort, fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days to obtain a fermented wort liquid. The liquid after fermentation was clarified by filtration and filled into containers.

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Abstract

The present invention addresses the problem of providing a beer-taste alcoholic beverage having an enhanced feeling of carbonation. As a means for solving the problem, provided is a beer-taste alcoholic beverage having a viscosity of 2.0 mPa s or more.

Description

ビールテイストアルコール飲料beer taste alcoholic beverage
 本発明はビールテイストアルコール飲料に関し、特に、アルコール濃度が低いビールテイスト低アルコール飲料に関する。 The present invention relates to a beer-taste alcoholic beverage, and particularly to a beer-taste low alcoholic beverage with a low alcohol concentration.
 ビールは4.5~5体積%のアルコール濃度を有し、炭酸ガスを含む飲料である。ビールは、例えば、飲用時のコク感及びボディ感等が満足感を与え、炭酸刺激等が爽快感を与えることで、嗜好性を実現している。 Beer is a beverage that has an alcohol concentration of 4.5 to 5% by volume and contains carbon dioxide gas. Beer achieves palatability by, for example, giving a feeling of satisfaction when drinking beer, such as the richness and body feeling, and giving a feeling of refreshment due to the stimulation of carbonic acid.
 近年、アルコール飲料に対する消費者の価値観は多様化し、カロリー、糖質又はアルコール度数を低減等したビールテイストアルコール飲料に対する需要が高まっている。その結果、ビールテイストアルコール飲料の機能ばかりでなく、嗜好性の重要性が高くなっている。 In recent years, consumer values regarding alcoholic beverages have diversified, and demand for beer-taste alcoholic beverages with reduced calories, sugar content, or alcohol content is increasing. As a result, not only the functionality of beer-taste alcoholic beverages but also their palatability have become more important.
 特許文献1には、麦汁中の非発酵性糖質の割合を高めることでアルコール濃度を低くした低アルコール発酵麦芽飲料において、最終製品のpHが3.5~4.4になるように有機酸を添加することを特徴とする低アルコール発酵麦芽飲料が記載されている。特許文献1の低アルコール発酵麦芽飲料は、甘味やその他の不自然な香味が突出することなく、通常のビール類と比べて遜色のないバランスの良い味感、風味を有するものである(要約)。特許文献1の低アルコール発酵麦芽飲料では、非発酵性糖質の甘味がビールと同様のコク味やボディ感を付与する一方で、有機酸の酸味が甘味等を突出させず、香味のバランスがとられている。 Patent Document 1 describes a low-alcohol fermented malt beverage in which the alcohol concentration is lowered by increasing the proportion of non-fermentable carbohydrates in the wort. A low-alcohol fermented malt beverage is described, which is characterized by the addition of acid. The low-alcohol fermented malt beverage of Patent Document 1 does not have prominent sweetness or other unnatural flavors, and has a well-balanced taste and flavor comparable to that of ordinary beers (Summary) . In the low-alcohol fermented malt beverage of Patent Document 1, the sweetness of non-fermentable carbohydrates imparts a rich taste and body feeling similar to that of beer, while the sourness of organic acids does not overpower the sweetness, resulting in a well-balanced flavor. It is taken.
 特許文献2には、5ppm以上のヒドロキシ酸エステル類を含有することを特徴とするビールテイスト飲料が記載されている。特許文献2のビールテイスト飲料は、炭酸ガス圧を高めることなく、炭酸刺激が増強されたものである(要約)。ヒドロキシ酸エステル類の苦味が炭酸による刺激と同時に感じられることによって、炭酸刺激と錯覚する結果、炭酸刺激が増強されるという作用が推察されている。 Patent Document 2 describes a beer-taste beverage characterized by containing 5 ppm or more of hydroxy acid esters. The beer-taste beverage of Patent Document 2 has enhanced carbonic acid stimulation without increasing carbon dioxide gas pressure (summary). It is speculated that the bitterness of hydroxy acid esters is felt at the same time as the stimulation by carbonic acid, giving the illusion of carbonic acid stimulation, and as a result, the stimulation of carbonic acid is enhanced.
特開2012-239460号公報JP2012-239460A 特開2016-47069号公報JP 2016-47069 Publication
 ビールテイストアルコール飲料は、低カロリー等の特有の機能を有するために、通常のビール類と対比した場合に、満足感又は爽快感に不十分な面が存在する。そのため、コク、ボディ感及び炭酸刺激等を増強して嗜好性を向上させることが望まれている。 Beer-taste alcoholic beverages have unique features such as low calorie content, so when compared to regular beer, they lack a sense of satisfaction or refreshment. Therefore, it is desired to improve palatability by enhancing richness, body feel, carbonic acid stimulation, etc.
 ビールテイストアルコール飲料の炭酸刺激を増強する手段として、炭酸ガス圧を高めることなどが知られている。しかしながら、炭酸ガス圧が高いビールテイストアルコール飲料は、市場に流通させるために、耐圧性が高い容器を使う必要が生じ、コストを要する。また、高められた炭酸ガス圧が充填設備の許容ガス圧を越える場合には、新たな設備導入が必要になる。また、炭酸刺激を増強する手段を講じる場合に、ビールテイストアルコール飲料の香味は影響を受けないことが好ましい。 Increasing the carbon dioxide pressure is known as a means to enhance the carbonic acid stimulation of beer-taste alcoholic beverages. However, beer-taste alcoholic beverages with high carbon dioxide gas pressure require the use of containers with high pressure resistance in order to be distributed on the market, which is costly. Furthermore, if the increased carbon dioxide pressure exceeds the allowable gas pressure of the filling equipment, new equipment will need to be installed. Furthermore, when taking measures to enhance carbonic acid stimulation, it is preferable that the flavor of the beer-taste alcoholic beverage is not affected.
 本発明は、前記課題を解決するものであり、その目的とするところは、炭酸ガス圧を高めることなく、香味が影響されることなく、炭酸刺激が増強されたビールテイストアルコール飲料を提供することにある。 The present invention solves the above-mentioned problems, and its purpose is to provide a beer-taste alcoholic beverage with enhanced carbonic acid stimulation without increasing carbon dioxide gas pressure and without affecting flavor. It is in.
 本発明には、以下の態様が含まれる。 The present invention includes the following aspects.
[態様1]
 2.0mPa・s以上、好ましくは2.0~5.0mPa・s、より好ましくは2.1~3.5mPa・s、更に好ましくは2.3~3.2mPa・sの粘度を有するビールテイストアルコール飲料。
[Aspect 1]
Beer taste having a viscosity of 2.0 mPa·s or more, preferably 2.0 to 5.0 mPa·s, more preferably 2.1 to 3.5 mPa·s, even more preferably 2.3 to 3.2 mPa·s alcoholic drinks.
[態様2]
 0.25MPa以下、好ましくは0.20~0.24MPa、より好ましくは0.22~0.24MPaの炭酸ガス圧を有する、態様1のビールテイストアルコール飲料。
[Aspect 2]
The beer-taste alcoholic beverage according to aspect 1, having a carbon dioxide gas pressure of 0.25 MPa or less, preferably 0.20 to 0.24 MPa, more preferably 0.22 to 0.24 MPa.
[態様3]
 4容量%以下、好ましくは1~4容量%、より好ましくは2~3.9容量%、更に好ましくは3~3.8容量%のアルコール濃度を有する、態様1又は2のビールテイストアルコール飲料。
[Aspect 3]
The beer-taste alcoholic beverage according to embodiment 1 or 2, having an alcohol concentration of 4% by volume or less, preferably 1 to 4% by volume, more preferably 2 to 3.9% by volume, even more preferably 3 to 3.8% by volume.
[態様4]
 容器詰め飲料である、態様1~3のいずれかのビールテイストアルコール飲料。
[Aspect 4]
The beer-taste alcoholic beverage according to any one of aspects 1 to 3, which is a packaged beverage.
[態様5]
 発酵麦芽飲料である、態様1~4のいずれかのビールテイストアルコール飲料。
[Aspect 5]
The beer-taste alcoholic beverage according to any one of aspects 1 to 4, which is a fermented malt beverage.
[態様6]
 95%以下、例えば30~95%、好ましくは、85%以下、例えば、35~85%、より好ましくは、35~60%、さらに好ましくは、40~50%の外観最終発酵度を有する、態様5のビールテイストアルコール飲料。
[Aspect 6]
Embodiments having an apparent final fermentation degree of 95% or less, such as 30 to 95%, preferably 85% or less, such as 35 to 85%, more preferably 35 to 60%, even more preferably 40 to 50%. 5 beer-taste alcoholic drinks.
[態様7]
 50~100重量%、より好ましくは67~100重量%の麦芽使用比率を有する、態様5又は6のビールテイストアルコール飲料。
[Aspect 7]
The beer-taste alcoholic beverage according to aspect 5 or 6, having a malt usage ratio of 50 to 100% by weight, more preferably 67 to 100% by weight.
[態様8]
 糖化に供される原料中のオーツ麦の含有量が15重量%以上、好ましくは20~80%、より好ましくは40~60%である、態様5~7のいずれかに記載のビールテイストアルコール飲料。
[Aspect 8]
The beer-taste alcoholic beverage according to any one of aspects 5 to 7, wherein the content of oats in the raw material to be subjected to saccharification is 15% by weight or more, preferably 20 to 80%, more preferably 40 to 60%. .
[態様9]
 粘度を2.0mPa・s以上、好ましくは2.0~5.0mPa・s、より好ましくは2.1~3.5mPa・s、更に好ましくは2.3~3.2mPa・sに調節することを含むビールテイストアルコール飲料の製造方法。
[Aspect 9]
Adjust the viscosity to 2.0 mPa·s or more, preferably 2.0 to 5.0 mPa·s, more preferably 2.1 to 3.5 mPa·s, even more preferably 2.3 to 3.2 mPa·s. A method for producing a beer-taste alcoholic beverage comprising:
[態様10]
 粘度を2.0mPa・s以上、好ましくは2.0~5.0mPa・s、より好ましくは2.1~3.5mPa・s、更に好ましくは2.3~3.2mPa・sに調節することを含むビールテイストアルコール飲料の炭酸刺激を増強する方法。
[Aspect 10]
Adjust the viscosity to 2.0 mPa·s or more, preferably 2.0 to 5.0 mPa·s, more preferably 2.1 to 3.5 mPa·s, even more preferably 2.3 to 3.2 mPa·s. A method for enhancing the carbonation stimulation of a beer-taste alcoholic beverage containing.
 本発明によれば、炭酸ガス圧を高めることなく、香味が影響されることなく、炭酸刺激が増強されたビールテイストアルコール飲料が提供される。 According to the present invention, a beer-taste alcoholic beverage with enhanced carbonic acid stimulation is provided without increasing carbon dioxide gas pressure and without affecting flavor.
<ビールテイストアルコール飲料>
 「ビールテイスト飲料」とは、ビールを想起させる香味及び炭酸刺激を有する炭酸飲料をいう。「ビール」とは、麦芽、ホップ及び水等を原料として、これらを酵母で発酵させて得られる発酵麦芽飲料をいう。ビールテイスト飲料のうちアルコールを含有するものを「ビールテイストアルコール飲料」という。「アルコール」という文言はエタノールを意味する。ビールテイスト飲料のうち、澱粉質原料に麦芽を含み、発酵過程を経て製造されたものを発酵麦芽飲料という。ビールテイストアルコール飲料には、ビール、発泡酒、ビール又は発泡酒をアルコール含有蒸留液と混和して得られたリキュール類等が含まれる。
<Beer-taste alcoholic beverage>
"Beer-taste beverage" refers to a carbonated beverage that has a flavor and carbonic acid stimulation reminiscent of beer. "Beer" refers to a fermented malt beverage obtained by fermenting malt, hops, water, etc. with yeast as raw materials. Among beer-taste drinks, those containing alcohol are referred to as "beer-taste alcoholic drinks." The term "alcohol" means ethanol. Among beer-taste drinks, those that contain malt as a starchy raw material and are produced through a fermentation process are called fermented malt drinks. Beer-taste alcoholic beverages include beer, low-malt beer, liqueurs obtained by mixing beer or low-malt beer with an alcohol-containing distillate, and the like.
 本発明のビールテイストアルコール飲料は、2.0mPa・s以上の粘度を有する。粘度は、物質の粘性の度合いを示す指標である。粘度は、液温8.5℃の状態のビールテイストアルコール飲料について粘度計を用いて測定した値をいう。粘度計の具体例としては、音叉振動式粘度計が挙げられる。ビールテイストアルコール飲料の粘度を所定範囲に調節することによって、飲用した際に感じる炭酸刺激を増強することができる。 The beer-taste alcoholic beverage of the present invention has a viscosity of 2.0 mPa·s or more. Viscosity is an indicator of the degree of viscosity of a substance. The viscosity refers to a value measured using a viscometer for a beer-taste alcoholic beverage at a liquid temperature of 8.5°C. A specific example of the viscometer is a tuning fork vibrating viscometer. By adjusting the viscosity of the beer-taste alcoholic beverage within a predetermined range, it is possible to enhance the carbonic acid stimulation felt when drinking the beverage.
 明確ではないけれども、ビールテイストアルコール飲料の粘度が増大した場合、液体が炭酸ガスを保持する力が増大し、飲用前の揮発が抑制されて、飲用後、口内で放出される炭酸ガスの濃度が高くなるという機構が推察される。 Although it is not clear, when the viscosity of a beer-taste alcoholic beverage increases, the ability of the liquid to retain carbon dioxide increases, and volatilization before drinking is suppressed, resulting in a lower concentration of carbon dioxide released in the mouth after drinking. The mechanism by which this increases is inferred.
 ビールテイストアルコール飲料の粘度は、好ましくは2.0~5.0mPa・s、より好ましくは2.1~3.5mPa・s、更に好ましくは2.3~3.2mPa・sである。 The viscosity of the beer-taste alcoholic beverage is preferably 2.0 to 5.0 mPa·s, more preferably 2.1 to 3.5 mPa·s, and still more preferably 2.3 to 3.2 mPa·s.
 ビールテイストアルコール飲料の粘度は、最終製品である飲料の粘度をいう。ビールテイストアルコール飲料の粘度の調節は、その製造方法に含まれるいずれかの工程において行うことができる。ビールテイストアルコール飲料の粘度を調節する方法は、例えば、原料の種類及び使用量を適宜変更する、又は増粘剤を適宜添加する等の方法が挙げられる。 The viscosity of a beer-taste alcoholic beverage refers to the viscosity of the final product, the beverage. The viscosity of the beer-taste alcoholic beverage can be adjusted in any step included in its production method. Examples of methods for adjusting the viscosity of the beer-taste alcoholic beverage include methods such as changing the type and amount of raw materials used, or adding a thickener as appropriate.
 増粘剤は、ビールテイストアルコール飲料に含有させた場合に、粘度が増大する物質をいう。増粘剤は、飲食品に使用可能なものであり、通常の使用量において香味に影響しない種類の物質であれば特に限定されないが、例えば、ペクチン、κ-カラギーナン、ジェランガム、キサンタンガム、ローカストビーンガム、寒天等の増粘多糖類が挙げられる。これらは、1種または2種以上を組み合わせて使用してもよい。増粘剤自体の香味がビールの香味により適しているという観点から、これらの中でも、ペクチン、キサンタンガム及び寒天が好ましい。なお、ペクチンとは、D-ガラクツロン酸を主成分とする多糖類であり、キサンタンガムとは、キサントモナス属細菌により発酵され生成される多糖類であり、寒天とは、アガロース及びアガロペクチンを主成分とする多糖類である。 A thickener is a substance that increases the viscosity when included in a beer-taste alcoholic beverage. Thickeners are not particularly limited as long as they are substances that can be used in foods and drinks and do not affect flavor in normal usage amounts, but include, for example, pectin, κ-carrageenan, gellan gum, xanthan gum, and locust bean gum. , agar, and other thickening polysaccharides. These may be used alone or in combination of two or more. Among these, pectin, xanthan gum and agar are preferred from the viewpoint that the flavor of the thickener itself is more suitable for the flavor of beer. Pectin is a polysaccharide whose main component is D-galacturonic acid, xanthan gum is a polysaccharide produced by fermentation by bacteria of the genus Xanthomonas, and agar is a polysaccharide whose main components are agarose and agaropectin. It is a polysaccharide.
 増粘剤の種類及び含有量は、ビールテイストアルコール飲料の粘度が前述した所定範囲内になるように調節される。一例として、ビールテイストアルコール飲料の増粘剤の含有量は、0.05~1w/v%、好ましくは0.1~0.9w/v%、より好ましくは0.2~0.8w/v%である。 The type and content of the thickener are adjusted so that the viscosity of the beer-taste alcoholic beverage falls within the aforementioned predetermined range. As an example, the content of the thickener in the beer-taste alcoholic beverage is 0.05 to 1 w/v%, preferably 0.1 to 0.9 w/v%, more preferably 0.2 to 0.8 w/v %.
 本発明のビールテイストアルコール飲料はビールと同程度のアルコール濃度を有してよい。本発明のビールテイストアルコール飲料は、具体的には、6体積(v/v)%以下、好ましくは1~6体積%、より好ましくは1体積%を超えて6体積%まで、更に好ましくは1.5~5.5体積%である。飲料中のアルコール濃度(即ち、エタノール濃度)の測定方法は当業者に広く知られており、例えば、「国税庁所定分析法」に記載の方法により測定することができる。 The beer-taste alcoholic beverage of the present invention may have an alcohol concentration comparable to that of beer. Specifically, the beer-taste alcoholic beverage of the present invention is 6% by volume (v/v) or less, preferably 1 to 6% by volume, more preferably more than 1% by volume to 6% by volume, even more preferably 1% by volume. .5 to 5.5% by volume. Methods for measuring alcohol concentration (i.e., ethanol concentration) in beverages are widely known to those skilled in the art, and can be measured, for example, by the method described in "National Tax Agency Specified Analysis Method."
 ある好ましい一形態において、本発明のビールテイストアルコール飲料は、通常のビールよりもアルコール濃度が低減されたビールテイスト低アルコール飲料である。ビールテイスト低アルコール飲料は、具体的には、4容量%以下、好ましくは1~4容量%、より好ましくは2~3.9容量%、更に好ましくは3~3.8容量%のアルコール濃度を有するビールテイストアルコール飲料をいう。 In one preferred embodiment, the beer-taste alcoholic beverage of the present invention is a beer-taste low-alcohol beverage with a lower alcohol concentration than normal beer. Specifically, the beer-taste low alcoholic beverage has an alcohol concentration of 4% by volume or less, preferably 1 to 4% by volume, more preferably 2 to 3.9% by volume, and even more preferably 3 to 3.8% by volume. An alcoholic beverage with a beer taste.
 ビールテイスト低アルコール飲料は通常のビールに比べて炭酸刺激を感じにくく、飲用時の爽快感が不十分である問題がある。ビールに含まれる炭酸は揮発して口腔から喉を刺激するが、アルコール濃度が低い場合、炭酸と共に揮発するアルコールの量が低下し、アルコールによる刺激作用が少なくなる結果、炭酸刺激の強さが低下すると考えられる。ビールテイストアルコール飲料のアルコール濃度を調節する方法は、例えば、麦汁発酵液の最終発酵度を低くする、又はアルコールの添加量を少量にする等の方法が挙げられる。 Beer-taste low-alcohol beverages have a problem in that they are less sensitive to carbonation than regular beer, and do not provide an adequate refreshing feeling when drinking. The carbonic acid contained in beer volatilizes and irritates the mouth and throat, but when the alcohol concentration is low, the amount of alcohol that volatilizes with the carbonic acid decreases, and the irritating effect of alcohol decreases, resulting in a decrease in the strength of the carbonic acid irritation. It is thought that then. Examples of methods for adjusting the alcohol concentration of the beer-taste alcoholic beverage include methods such as lowering the final fermentation degree of the wort fermentation liquid, or reducing the amount of alcohol added.
<ビールテイストアルコール飲料の製造方法>
 本発明のビールテイストアルコール飲料の製造方法を、以下に具体的に説明する。本発明のビールテイストアルコール飲料は、例えば、麦汁をビール酵母で発酵させて製造することができる。
<Method for producing beer-taste alcoholic beverage>
The method for producing a beer-taste alcoholic beverage of the present invention will be specifically explained below. The beer-taste alcoholic beverage of the present invention can be produced, for example, by fermenting wort with beer yeast.
<麦汁>
 麦汁は澱粉質原料の糖化液及び水を含む液体をいう。麦汁は、例えば、次のようにして製造する。
<wort>
Wort is a liquid containing water and a saccharified solution of starchy raw materials. Wort is produced, for example, as follows.
 まず、主原料として麦芽の破砕物、大麦等の副原料、及び温水を仕込槽に加えて混合してマイシェを調製する。この場合、得られるビールテイストアルコール飲料は発酵麦芽飲料になる。発酵麦芽飲料に該当しないビールテイストアルコール飲料を製造する場合は、主原料を使用しないか、主原料として麦芽以外の穀類を使用する。 First, crushed malt as the main raw material, auxiliary raw materials such as barley, and hot water are added to a brewing tank and mixed to prepare maiche. In this case, the resulting beer-taste alcoholic beverage becomes a fermented malt beverage. When producing beer-taste alcoholic beverages that do not fall under the category of fermented malt beverages, the main raw material is either not used or grains other than malt are used as the main raw material.
 マイシェの調製は、常法により行うことができ、例えば、はじめに35~60℃で20~90分間保持することにより原料に由来するタンパク質をアミノ酸などへ分解し、糖化工程へ移行する。その際、必要に応じて、主原料と副原料以外に、糖化酵素、並びにスパイス、ハーブ類及び香料等の香味成分等が添加される。 Preparation of maiche can be carried out by a conventional method, for example, first, the protein derived from the raw material is decomposed into amino acids etc. by holding it at 35 to 60°C for 20 to 90 minutes, and then the process proceeds to the saccharification step. At that time, in addition to the main raw materials and auxiliary raw materials, saccharifying enzymes and flavor components such as spices, herbs, and fragrances are added as necessary.
 その後、該マイシェを徐々に昇温して所定の温度で一定期間保持することにより、麦芽由来の酵素やマイシェに添加した酵素を利用して、澱粉質を糖化させる。糖化処理時の温度や時間は、用いる酵素の種類やマイシェの量、目的とする麦汁のビール酵母発酵液(以下、単に「麦汁発酵液」ということがある。)の品質等を考慮して、適宜決定することができ、例えば、60~72℃にて30~90分間保持することにより行うことができる。糖化処理後、76~78℃で10分間程度保持した後、マイシェを濾過することにより、透明な糖液を得る。 Thereafter, the temperature of the mache is gradually raised and held at a predetermined temperature for a certain period of time to saccharify the starch using malt-derived enzymes and enzymes added to the mache. The temperature and time during the saccharification treatment should be determined by taking into account the type of enzyme used, the amount of mache, the quality of the target wort beer yeast fermentation liquid (hereinafter sometimes simply referred to as "wort fermentation liquid"), etc. The temperature can be determined as appropriate, for example, by holding the temperature at 60 to 72°C for 30 to 90 minutes. After the saccharification treatment, the mixture is maintained at 76 to 78°C for about 10 minutes, and then the mache is filtered to obtain a transparent sugar solution.
 発酵麦芽飲料を製造する場合、糖化に供される原料(本明細書中、「澱粉質原料」ということがある。)は麦芽を含む。糖化に供される原料中の麦芽の含有量は、特に限定されないが、25%以上、好ましくは50%以上、より好ましくは67%以上である。糖化に供される原料は麦芽使用比率100%であってもよい。尚、糖化に供される原料に対する麦芽の割合(重量(w/w)%)を麦芽使用比率という。一般に、麦芽使用比率が高いほど、得られる麦汁発酵液の麦芽由来の旨味、コク及び飲みごたえが強くなる。 When producing a fermented malt beverage, the raw material subjected to saccharification (herein sometimes referred to as "starchy raw material") contains malt. The content of malt in the raw material to be subjected to saccharification is not particularly limited, but is 25% or more, preferably 50% or more, and more preferably 67% or more. The raw material subjected to saccharification may have a malt usage ratio of 100%. The ratio of malt (weight (w/w) %) to the raw materials to be subjected to saccharification is referred to as the malt usage ratio. Generally, the higher the malt usage ratio, the stronger the malt-derived flavor, richness, and drinkability of the resulting wort fermentation liquid will be.
 糖化に供される原料は、好ましくは、オーツ麦を含む。糖化に供される原料中のオーツ麦の含有量は、特に限定されないが、15%以上、好ましくは20~80%、より好ましくは40~60%である。糖化に供される原料中のオーツ麦の含有量が高いほど、得られるビールテイストアルコール飲料の粘度が高くなる。オーツ麦は、好ましくは、発芽させて麦芽をつくるという製麦処理を施したものを使用する。 The raw material subjected to saccharification preferably contains oats. The content of oats in the raw material to be subjected to saccharification is not particularly limited, but is 15% or more, preferably 20 to 80%, more preferably 40 to 60%. The higher the oat content in the raw materials subjected to saccharification, the higher the viscosity of the resulting beer-taste alcoholic beverage. The oats used are preferably those that have been subjected to a malting process to produce malt through germination.
 副原料とは、麦芽とホップ以外の原料を意味する。該副原料として、例えば、大麦、小麦、コーンスターチ、コーングリッツ、米、こうりゃん等の澱粉原料や、液糖や砂糖等の糖質原料がある。ここで、液糖とは、澱粉質を酸又は糖化酵素により分解、糖化して製造されたものであり、主にグルコース、マルトース、マルトトリオース等が含まれている。その他、香味を付与又は改善することを目的として用いられるスパイス類、ハーブ類、及び果物等も、副原料に含まれる。 Auxiliary raw materials refer to raw materials other than malt and hops. Examples of the auxiliary raw materials include starch raw materials such as barley, wheat, cornstarch, corn grits, rice, and soybean, and carbohydrate raw materials such as liquid sugar and sugar. Here, liquid sugar is produced by decomposing and saccharifying starch with an acid or a saccharifying enzyme, and mainly contains glucose, maltose, maltotriose, and the like. In addition, spices, herbs, fruits, etc. used for the purpose of imparting or improving flavor are also included in the auxiliary raw materials.
 糖化酵素とは、澱粉質を分解して糖を生成させる酵素を意味する。該糖化酵素として、好ましくは、トランスグルコシダーゼを使用する。トランスグルコシダーゼを使用した場合、麦汁に含まれる非資化性糖の量を変化させることができ、麦汁発酵液の最終発酵度及び麦汁発酵液のエキス濃度を調節することが容易になる。糖化酵素として、例えば、α-アミラーゼ、グルコアミラーゼ、プルナラーゼ等を、トランスグルコシダーゼと組み合わせて使用してもよい。 Saccharifying enzyme refers to an enzyme that decomposes starch to produce sugar. Transglucosidase is preferably used as the saccharifying enzyme. When transglucosidase is used, the amount of non-assimilable sugars contained in the wort can be changed, making it easy to adjust the final fermentation degree of the wort fermentation liquid and the extract concentration of the wort fermentation liquid. . As the saccharifying enzyme, for example, α-amylase, glucoamylase, prunalase, etc. may be used in combination with transglucosidase.
 トランスグルコシダーゼとしては、糖転移反応に対する触媒活性を有する酵素であれば、特に限定されるものではなく、各種生物由来のトランスグルコシダーゼを使用することができる。例えば、市販されているトランスグルコシダーゼのうち、いずれの酵素を用いても良く、またこれらを組み合わせて用いることもできる。 The transglucosidase is not particularly limited as long as it has catalytic activity for transglycosylation reactions, and transglucosidases derived from various organisms can be used. For example, any commercially available transglucosidase may be used, or a combination of these may be used.
 マイシェへのトランスグルコシダーゼの添加は、仕込工程終了時点までに添加したトランスグルコシダーゼによる酵素反応が充分に行われる時点であれば特に限定されるものではない。例えばトランスグルコシダーゼは、マイシェの調製時点において、麦芽等の発酵原料とともに添加されてもよく、糖化反応の途中に添加してもよい。本発明においては、トランスグルコシダーゼによる酵素反応を充分に進行させられるため、マイシェの調製時点又は仕込工程の早い段階でトランスグルコシダーゼを添加することが好ましく、マイシェの調製時点に添加することがより好ましい。 The addition of transglucosidase to mashed potatoes is not particularly limited as long as the enzymatic reaction by the added transglucosidase is sufficiently carried out by the end of the preparation process. For example, transglucosidase may be added together with fermentation raw materials such as malt at the time of preparing mache, or may be added during the saccharification reaction. In the present invention, in order to allow the enzymatic reaction by transglucosidase to proceed sufficiently, transglucosidase is preferably added at the time of preparing mache or at an early stage of the preparation process, and more preferably added at the time of preparing mache.
 麦汁煮沸の操作は、ビールを製造する際に通常行われる方法及び条件に従って行えばよい。例えば、pHを調整した糖液を煮沸釜に移し、煮沸する。糖液の煮沸開始時から、ワールプール静置の間に、ホップを添加する。ホップとして、ホップエキス又はホップから抽出した成分を使用してもよい。 The wort boiling operation may be performed according to the method and conditions normally used when producing beer. For example, a pH-adjusted sugar solution is transferred to a boiling pot and boiled. Hops are added from the time the sugar solution starts boiling to the time it is left in the whirlpool. As the hop, a hop extract or a component extracted from hops may be used.
 糖液は次いでワールプールと呼ばれる沈殿槽に移し、煮沸により生じたホップ粕や凝固したタンパク質等を除去した後、プレートクーラーにより適切な温度まで冷却する。上記麦汁煮沸までの操作により、本願発明でいう麦汁が得られる。糖化酵素としてトランスグルコシダーゼを使用した場合、得られた麦汁をトランスグルコシダーゼ処理麦汁という。 The sugar solution is then transferred to a settling tank called a whirlpool, and after removing hop residue and coagulated proteins produced by boiling, it is cooled to an appropriate temperature using a plate cooler. By the above-mentioned operation up to wort boiling, wort as referred to in the present invention is obtained. When transglucosidase is used as the saccharifying enzyme, the resulting wort is called transglucosidase-treated wort.
 非資化性糖質とは、ビール酵母が、アルコール発酵等を行うための栄養源として利用しない糖質をいう。本発明で使用するビール酵母は、グルコース、フルクトース、マルトース、マルトトリオースを資化する。それゆえ、これら以外の糖質を総称して非資化性糖質という。トランスグルコシダーゼ処理麦汁に含まれる非資化性糖質の具体例には、イソマルトース、イソマルトトリオース、パノース、4糖以上のオリゴ糖及び非資化性多糖が含まれる。非資化性糖質は、後述する発酵工程及びアルコール除去工程を経た後も、残存している。 Non-assimilable carbohydrates refer to carbohydrates that are not used by brewer's yeast as a nutrient source for alcohol fermentation, etc. The brewer's yeast used in the present invention assimilates glucose, fructose, maltose, and maltotriose. Therefore, carbohydrates other than these are collectively called non-assimilated carbohydrates. Specific examples of non-assimilable carbohydrates contained in the transglucosidase-treated wort include isomaltose, isomaltotriose, panose, oligosaccharides of 4 or more sugars, and non-assimilable polysaccharides. The non-assimilable carbohydrates remain even after the fermentation process and alcohol removal process described below.
 マイシェを作製する際のトランスグルコシダーゼの使用量は、その種類及び反応条件を考慮して、麦汁発酵液の最終発酵度、又は麦汁発酵液のエキス濃度が上記範囲になるように、適宜調節される。一般には、トランスグルコシダーゼの使用量は、マイシェの固形分を基準にして0.05~20g/kg、好ましくは0.1~7g/kg、より好ましくは0.25~5g/kgである。 The amount of transglucosidase used when producing maishe is adjusted as appropriate, taking into account the type and reaction conditions, so that the final fermentation degree of the wort fermentation liquid or the extract concentration of the wort fermentation liquid is within the above range. be done. Generally, the amount of transglucosidase used is 0.05 to 20 g/kg, preferably 0.1 to 7 g/kg, more preferably 0.25 to 5 g/kg, based on the solid content of the mache.
 麦汁は、5~30°Pの麦汁エキス濃度を有するように水分量を調節する。麦汁エキス濃度が5°P未満である場合、得られる麦汁発酵液の複雑味及び爽やかな香気が不十分になることがあり、30°Pを超える場合、得られる麦汁発酵液の複雑味及び香気が強すぎて、ビールらしい香味が損なわれることがある。麦汁の麦汁エキス濃度は、好ましくは7~20°P、より好ましくは8~15°Pである。 Adjust the water content of the wort so that it has a wort extract concentration of 5 to 30°P. If the wort extract concentration is less than 5°P, the complexity and refreshing aroma of the resulting wort fermentation liquid may be insufficient; if it exceeds 30°P, the complexity and refreshing aroma of the resulting wort fermentation liquid may be insufficient. The taste and aroma may be too strong and the beer-like flavor may be lost. The wort extract concentration of the wort is preferably 7-20°P, more preferably 8-15°P.
<麦汁発酵液>
 得られた麦汁は、ビール酵母により発酵させる。麦汁の発酵は常法に従って行えばよい。例えば、冷却した麦汁にビール酵母を接種して、発酵タンクに移し、アルコール発酵を行う。
<wort fermentation liquid>
The obtained wort is fermented with brewer's yeast. Fermentation of wort may be carried out according to conventional methods. For example, cooled wort is inoculated with brewer's yeast, transferred to a fermentation tank, and alcoholic fermentation is performed.
 麦汁発酵液の外観最終発酵度は、ある一形態において、95%以下、例えば、30~95%である。麦汁発酵液の外観最終発酵度が30%未満である場合、複雑味が弱く、甘味が十分に抑制されなくなることがあり、得られるビールテイストアルコール飲料の甘味又はコク感が後味として残り、キレ感及び飲用後の爽快感が不十分になる。麦汁発酵液の外観最終発酵度が95%を超える場合、得られるビールテイストアルコール飲料の飲みごたえ又はコク感が不十分、また、酸味が強すぎて、ビールらしい香味が損なわれることがある。麦汁発酵液の外観最終発酵度は、好ましくは、85%以下、例えば、35~85%、より好ましくは、35~60%、さらに好ましくは、40~50%である。 In one embodiment, the appearance and final fermentation degree of the wort fermentation liquid is 95% or less, for example, 30 to 95%. Appearance of the fermented wort If the final fermentation degree is less than 30%, the complex flavor may be weak and the sweetness may not be sufficiently suppressed, and the sweetness or richness of the resulting beer-taste alcoholic beverage may remain as an aftertaste, resulting in a sharp taste. The sensation and refreshing feeling after drinking become insufficient. If the appearance of the wort fermented liquid exceeds 95%, the resulting beer-taste alcoholic beverage may have insufficient texture or richness, and may be too sour, resulting in a loss of beer-like flavor. The final fermentation degree in appearance of the wort fermented liquid is preferably 85% or less, for example, 35 to 85%, more preferably 35 to 60%, and still more preferably 40 to 50%.
 発酵度とは、発酵後のビールにおいて、どれだけ発酵が進んだか、発酵の進み方を示す指標である。最終発酵度とは、原麦汁エキスに対して、ビール酵母が資化可能なエキスの割合を意味する。ここで、ビール酵母が資化可能なエキスとは、原麦汁エキスから、製品ビールに含まれるエキス(即ち、ビール酵母が利用可能なエキスをすべて発酵させた後に残存するエキス(最終エキスという))を差し引いたものである。外観最終発酵度とは、最終エキスの値に、外観エキスを使用して計算した最終発酵度をいう。 The degree of fermentation is an index that indicates how much fermentation has progressed in beer after fermentation. The final fermentation degree refers to the ratio of extract that can be assimilated by brewer's yeast to the original wort extract. Here, the extract that can be assimilated by brewer's yeast refers to the extract contained in the product beer from the original wort extract (i.e., the extract that remains after fermenting all the extracts that can be used by brewer's yeast (referred to as the final extract). ) is deducted. The appearance final fermentation degree refers to the final fermentation degree calculated using the appearance extract as the value of the final extract.
 尚、エキスとは不揮発性固形分をいう。エキスという文言は、文脈に応じて、不揮発性固形分そのもの、不揮発性固形分の量、又は不揮発性固形分の濃度を意味する。 Note that the extract refers to nonvolatile solid content. The term extract means, depending on the context, the non-volatile solids themselves, the amount of non-volatile solids, or the concentration of non-volatile solids.
 外観エキス濃度とは、アルコール(エタノール)を含んだ状態の液の不揮発性固形物の濃度(°P)をいう。麦汁発酵液の比重及びアルコール濃度は、BCOJビール分析法、8.4.3及び8.3.6(いずれもアルコライザー法)により、それぞれ測定することができる。外観エキス濃度は、これらの測定値を使用して、BCOJビール分析法、8.5エキス関係計算法により、計算することができる。 The appearance extract concentration refers to the concentration (°P) of nonvolatile solids in a liquid containing alcohol (ethanol). The specific gravity and alcohol concentration of the wort fermentation liquid can be measured by the BCOJ beer analysis method, 8.4.3 and 8.3.6 (both alcoholizer methods). The appearance extract concentration can be calculated using these measured values by the BCOJ beer analysis method and the 8.5 extract relationship calculation method.
 麦汁発酵液の外観最終発酵度Vendは、例えば下記式(1)により、求めることができる。 The appearance and final fermentation degree Vend of the fermented wort liquid can be determined, for example, by the following formula (1).
Vend(%)={(P-Eend)/P}×100  (1)
[式中、Pは原麦汁エキスであり、Eendは、外観最終エキスである。]
Vend (%) = {(P-Eend)/P}×100 (1)
[Wherein, P is the raw wort extract, and Eend is the final appearance extract. ]
 原麦汁エキスPは、製品ビールのアルコール濃度とエキスの値から、Ballingの式に従い、理論上アルコール発酵前の麦汁エキスの値を逆算するものである。具体的には、Analytica-EBC(9.4)(2007)に示される方法により、求めることができる。また、外観最終エキスEendはビールをフラスコに採取し、新鮮な圧搾酵母を多量に添加し、25℃で攪拌しながら、エキスの値がこれ以上低下しなくなるまで発酵させて(24時間)、残存ビール中の外観エキスの値を測定することにより、求めることができる。 The raw wort extract P is calculated by theoretically back-calculating the value of the wort extract before alcoholic fermentation from the alcohol concentration of the product beer and the value of the extract according to Balling's formula. Specifically, it can be determined by the method shown in Analytica-EBC (9.4) (2007). In addition, the appearance final extract Eend is obtained by collecting beer in a flask, adding a large amount of freshly pressed yeast, and fermenting it at 25°C with stirring until the extract value no longer decreases (24 hours). It can be determined by measuring the value of appearance extract in beer.
 外観最終エキスEendは、最終エキスのアルコールを含んだ比重から計算されるため、マイナスの値を示すことがある。その結果、外観最終発酵度は100%を超える場合がある。 Appearance final extract Eend is calculated from the specific gravity of the final extract including alcohol, so it may show a negative value. As a result, the apparent final fermentation degree may exceed 100%.
 外観最終発酵度は、例えば、糖化条件、原料を糖化させる際の酵素の使用有無、及び、原材料の種類や配合量などを調整することにより、制御することができる。例えば、マイシェに含まれる資化性糖質の量を低下させることで、外観最終発酵度を低下させることができる。 The final fermentation degree in appearance can be controlled by, for example, adjusting the saccharification conditions, whether or not enzymes are used when saccharifying the raw materials, and the types and amounts of raw materials. For example, by lowering the amount of assimilable carbohydrates contained in mache, the final fermentation degree in appearance can be lowered.
 発酵終了後、さらに、熟成工程として、得られた麦汁発酵液を、貯酒タンク中で熟成させ、0℃程度の低温条件下で貯蔵し安定化させる。次いで濾過工程として、熟成後の麦汁発酵液を濾過することにより酵母及びタンパク質等を除去する。 After completion of fermentation, the resulting wort fermentation liquid is further matured in a sake storage tank as a maturing step, and then stored and stabilized under low temperature conditions of about 0°C. Next, in a filtration step, the aged wort fermented liquid is filtered to remove yeast, proteins, and the like.
 麦汁発酵液は、3~15°Pの真正エキス濃度を有する。麦汁発酵液の真正エキス濃度が不足する場合は、麦汁発酵液及び得られる低アルコールビールテイスト発酵麦芽飲料の飲みごたえ又はコク感が不十分になることがある。麦汁発酵液の真正エキス濃度が過剰である場合は、複雑味が弱く、甘味が十分に抑制されなくなることがあり、得られる低アルコールビールテイスト発酵麦芽飲料の甘味又はコク感が後味として残り、キレ感及び飲用後の爽快感が不十分になる。麦汁発酵液の真正エキス濃度は、好ましくは5~10°P、より好ましくは7~9°Pである。 The wort fermentation liquor has an authentic extract concentration of 3-15°P. When the authentic extract concentration of the wort fermentation liquid is insufficient, the drinking sensation or richness of the wort fermentation liquid and the resulting low alcohol beer-taste fermented malt beverage may become insufficient. If the true extract concentration of the fermented wort liquid is excessive, the complex taste may be weak and the sweetness may not be sufficiently suppressed, and the sweetness or richness of the resulting low-alcohol beer-taste fermented malt beverage may remain as an aftertaste. The sharp feeling and the refreshing feeling after drinking become insufficient. The authentic extract concentration of the wort fermentation liquid is preferably 5-10°P, more preferably 7-9°P.
 真正エキス濃度とは、飲料に含まれる不揮発性固形物の質量濃度(°P)をいう。真正エキス濃度は、BCOJビール分析法、8.4.3(アルコライザー法)により、測定することができる。 The authentic extract concentration refers to the mass concentration (°P) of nonvolatile solids contained in the beverage. The authentic extract concentration can be measured by BCOJ beer analysis method, 8.4.3 (alcolyzer method).
<ビールテイストアルコール飲料>
 麦汁発酵液は、必要に応じて、pH調整剤を添加してpHを下げる。麦汁発酵液のアルコール濃度が低く、ビールテイスト低アルコール飲料を得る場合等では、ビールテイスト低アルコール飲料の静菌性が不足し、保存安定性が低下するおそれがある。pHを十分に下げることで、ビールテイスト低アルコール飲料を市販する際に必要な静菌性及び保存安定性が付与される。この場合、具体的なpHの上限値は4.2未満、好ましくはpH4.1未満、より好ましくは、4.0未満である。一方、具体的なpHの下限値は3.0以上、好ましくは3.3以上、より好ましくは3.6以上である。
<Beer-taste alcoholic beverage>
If necessary, a pH adjuster is added to the wort fermentation liquid to lower the pH. In cases where the alcohol concentration of the wort fermentation liquid is low and a beer-taste low-alcohol beverage is obtained, the bacteriostatic properties of the beer-taste low-alcohol beverage may be insufficient and storage stability may be reduced. Sufficiently lowering the pH provides the bacteriostatic properties and storage stability necessary for marketing beer-taste low-alcohol beverages. In this case, the specific upper limit of pH is less than 4.2, preferably less than 4.1, more preferably less than 4.0. On the other hand, the specific lower limit of pH is 3.0 or more, preferably 3.3 or more, more preferably 3.6 or more.
 pH調整剤の種類は限定されない。食品添加物に限らず、例えば飲料及び食品やそれらの製造工程に使用することができる酸、それらの塩及びpH低下能を有するビール原料であれば、pH調整剤として使用することができる。pH低下能を有するビール原料として、例えば、サワーモルト、濃色麦芽等がある。好ましいpH調整剤はフィチン酸、クエン酸、乳酸、乳酸菌、リン酸、リンゴ酸、無水亜硫酸、酒石酸、グルコン酸、酢酸、コハク酸、アジピン酸、イタコン酸、フマル酸及びこれらの組み合わせである。より好ましいpH調整剤はフィチン酸、乳酸、乳酸菌、リン酸、リンゴ酸、無水亜硫酸、酒石酸及びこれらの組み合わせである。ビールテイスト飲料の香味に与える影響を考慮すると、これらの中でも酸味が少ないフィチン酸が最も好ましい。 The type of pH adjuster is not limited. Not limited to food additives, for example, acids and salts thereof that can be used in beverages and foods, their manufacturing processes, and beer raw materials having pH lowering ability can be used as pH adjusters. Examples of beer raw materials having the ability to lower pH include sour malt, dark malt, and the like. Preferred pH adjusting agents are phytic acid, citric acid, lactic acid, lactic acid bacteria, phosphoric acid, malic acid, sulfite anhydride, tartaric acid, gluconic acid, acetic acid, succinic acid, adipic acid, itaconic acid, fumaric acid, and combinations thereof. More preferred pH adjusting agents are phytic acid, lactic acid, lactic acid bacteria, phosphoric acid, malic acid, sulfite anhydride, tartaric acid, and combinations thereof. Considering its influence on the flavor of beer-taste beverages, phytic acid, which has less sourness, is the most preferred among these.
 麦汁発酵液には、必要に応じて、カーボネーション工程によって炭酸ガスを添加する。そのことで、ビールテイストアルコール飲料の炭酸刺激が増強される。炭酸ガスの添加量はビールと同様の発泡性になるように調整する。ビールテイストアルコール飲料の炭酸ガスのガス圧は、充填される容器の耐圧性または充填設備の許容ガス圧を超えない範囲内に調節される。炭酸ガス圧は、例えば、0.25MPa以下、好ましくは0.20~0.24MPa、より好ましくは0.22~0.24MPaである。本明細書でいう炭酸ガス溶液の炭酸ガス圧は、常法に従い、液温20℃の条件にて測定された値を意味している。 If necessary, carbon dioxide gas is added to the wort fermentation liquid through a carbonation process. This enhances the carbonation stimulation of the beer-taste alcoholic beverage. The amount of carbon dioxide gas added is adjusted so that the foaming properties are similar to those of beer. The gas pressure of the carbon dioxide gas in the beer-taste alcoholic beverage is adjusted within a range that does not exceed the pressure resistance of the container to be filled or the allowable gas pressure of the filling equipment. The carbon dioxide pressure is, for example, 0.25 MPa or less, preferably 0.20 to 0.24 MPa, more preferably 0.22 to 0.24 MPa. The carbon dioxide pressure of a carbon dioxide solution as used herein means a value measured at a liquid temperature of 20° C. according to a conventional method.
 このようにして得られた本発明のビールテイストアルコール飲料は、所定の容器に充填され、製品として市場に流通する。ビールテイストアルコール飲料の容器詰め方法としては、特に限定されず、当業者に周知の容器詰め方法を用いることができる。容器詰め工程によって、本発明のビールテイストアルコール飲料は容器に充填・密閉される。容器詰め工程には、いずれの形態・材質の容器を用いてもよく、容器の例としては、ビン、缶、樽またはペットボトルが挙げられるが、特に持ち運びが容易であるとの観点から、缶、ビンやペットボトルが好ましい。 The beer-taste alcoholic beverage of the present invention thus obtained is filled into a predetermined container and distributed on the market as a product. The method for packaging the beer-taste alcoholic beverage is not particularly limited, and any method known to those skilled in the art can be used. In the container filling step, the beer-taste alcoholic beverage of the present invention is filled into a container and sealed. In the container filling process, containers of any form and material may be used. Examples of containers include bottles, cans, barrels, and PET bottles. , bottles or plastic bottles are preferred.
 以下の実施例により本発明を更に具体的に説明するが、本発明は、これらに限定されるものではない。実施例において、濃度、含有量の単位は特に断らない限り重量基準である。 The present invention will be explained in more detail with reference to the following examples, but the present invention is not limited thereto. In the examples, the units of concentration and content are based on weight unless otherwise specified.
<比較例1>
[ビールテイスト飲料の製造]
仕込槽に大麦麦芽400kg、コーンスターチ160kg、温水1500リットルを導入し、65℃にて60分間糖化を行った。この糖化液を濾過槽であるロイターにて濾過し、その後煮沸釜に移して60分間煮沸し、ホップ8kgを添加した。煮沸後、蒸発分の温水を追加し、2500Lとした。ワールプール槽にて熱トルーブを除去した後、プレートクーラーを用いて8℃まで冷却し、麦汁を得た。
<Comparative example 1>
[Manufacture of beer-taste beverages]
400 kg of barley malt, 160 kg of cornstarch, and 1500 liters of hot water were introduced into a preparation tank, and saccharification was performed at 65° C. for 60 minutes. This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 8°C using a plate cooler to obtain wort.
 得られた麦汁にビール酵母を加え、7日間10℃前後で発酵させた後、ビール酵母を除去した。タンクを移し替えて7日間熟成させた後、-1℃付近まで冷却し14日間安定化させた。麦汁発酵液は濾過にて清澄化し、加水して、原麦汁エキスを11.19°Pに調整した。これをビールテイスト飲料として容器に充填した。 Brewer's yeast was added to the obtained wort and fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days. The wort fermentation liquid was clarified by filtration and water was added to adjust the original wort extract to 11.19°P. This was filled into a container as a beer-taste drink.
[特性の測定]
 常法に従って、得られたビールテイスト飲料の原麦汁エキス濃度、外観最終発酵度、真正エキス濃度、炭酸ガス圧及びアルコール濃度を測定した。また、次の手順によりビールテイスト飲料の粘度を測定した。
[Measurement of characteristics]
The original wort extract concentration, appearance final fermentation degree, true extract concentration, carbon dioxide pressure, and alcohol concentration of the resulting beer-taste beverage were measured according to conventional methods. In addition, the viscosity of the beer-taste beverage was measured according to the following procedure.
 即ち、前処理として飲料の炭酸ガスを揮発させた。次いで、恒温槽にて液温を8.5℃に調節した。調温した液を音叉振動式粘度計(エー・アンド・デイ社製「SV-10A」(商品名))にセットして、その粘度を測定した。特性の測定結果は表1に示す。 That is, carbon dioxide gas in the beverage was volatilized as a pretreatment. Next, the liquid temperature was adjusted to 8.5°C in a constant temperature bath. The temperature-controlled liquid was set in a tuning fork vibrating viscometer ("SV-10A" (trade name) manufactured by A&D Co., Ltd.), and its viscosity was measured. The measurement results of the characteristics are shown in Table 1.
[炭酸刺激強度の測定]
 炭酸刺激強度は官能試験にて測定した。つまり、訓練されたビール専門パネリスト5名がビールテイスト飲料を試飲し、感じられた官能について後述の評価基準に従って1~10点の10段階で採点した。パネリスト全員の採点の平均値を各評価項目の評点とした。なお、評価に供されるサンプルは、液温を約4℃に調節した。評価点が5点以上の場合に良いと判断される。
[Measurement of carbonic acid stimulus intensity]
Carbonic acid stimulation intensity was measured by a sensory test. That is, five trained panelists specializing in beer sampled the beer-taste beverages and scored them on a scale of 1 to 10 on a scale of 1 to 10 based on the sensory sensations described below. The average score of all panelists was used as the score for each evaluation item. Note that the liquid temperature of the samples used for evaluation was adjusted to about 4°C. It is judged to be good if the evaluation score is 5 points or more.
(評価基準)
 炭酸ガス圧を調整した炭酸水を調製し、炭酸刺激強度の評価基準として使用した。











































(Evaluation criteria)
Carbonated water with adjusted carbon dioxide gas pressure was prepared and used as an evaluation standard for carbonic acid stimulation strength.











































Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
<比較例2>
[ビールテイスト飲料の製造]
 仕込槽に大麦麦芽500kg、温水1500リットルを導入し、65℃にて10分間糖化を行った。この糖化液を濾過槽であるロイターにて濾過し、その後煮沸釜に移して60分間煮沸し、ホップ8kgを添加した。煮沸後、蒸発分の温水を追加し、2500Lとした。ワールプール槽にて熱トルーブを除去した後、プレートクーラーを用いて8℃まで冷却し、麦汁を得た。
<Comparative example 2>
[Manufacture of beer-taste beverages]
500 kg of barley malt and 1500 liters of hot water were introduced into a preparation tank, and saccharification was performed at 65° C. for 10 minutes. This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 8°C using a plate cooler to obtain wort.
 得られた麦汁にビール酵母を加え、7日間10℃前後で発酵させた後、ビール酵母を除去した。タンクを移し替えて7日間熟成させた後、-1℃付近まで冷却し14日間安定化させた。麦汁発酵液は濾過にて清澄化し、加水して、原麦汁エキスを9.06°Pに調整した。これをビールテイスト飲料として容器に充填した。 Brewer's yeast was added to the obtained wort and fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days. The wort fermentation liquid was clarified by filtration and water was added to adjust the original wort extract to 9.06°P. This was filled into a container as a beer-taste drink.
 比較例1と同様にしてビールテイスト飲料の特性を測定した。結果を表2に示す。 The characteristics of the beer-taste beverage were measured in the same manner as in Comparative Example 1. The results are shown in Table 2.
<実施例1~4>
[ビールテイスト飲料の製造]
 増粘剤として、ユニテックフーズ社製の粉末ペクチンを準備した。比較例2で得られたビールテイスト飲料に前記ペクチンを添加し溶解させ、粘度を増大させてビールテイスト飲料を製造し、容器に充填し、比較例1と同様にして特性を測定した。各実施例についてのペクチンの添加量、測定された特性を、表2に示す。
<Examples 1 to 4>
[Manufacture of beer-taste beverages]
Powdered pectin manufactured by Unitec Foods was prepared as a thickener. The pectin was added and dissolved in the beer-taste beverage obtained in Comparative Example 2 to increase the viscosity to produce a beer-taste beverage, which was filled into containers and measured for characteristics in the same manner as in Comparative Example 1. Table 2 shows the amount of pectin added and the measured properties for each example.
<実施例5>
[ビールテイスト飲料の製造]
 仕込槽に大麦麦芽400kg、コーンスターチ160kg、温水1500リットル及びトランスグルコシダーゼ(天野エンザイム株式会社製「トランスグルコシダーゼL」(商品名))3.0kgを導入し、60℃から76℃の範囲の温度で糖化を行った。この糖化液を濾過槽であるロイターにて濾過し、その後煮沸釜に移して60分間煮沸し、ホップ8kgを添加した。煮沸後、蒸発分の温水を追加し、2500Lとした。ワールプール槽にて熱トルーブを除去した後、プレートクーラーを用いて8℃まで冷却し、トランスグルコシダーゼ処理麦汁を得た。
<Example 5>
[Manufacture of beer-taste beverages]
400 kg of barley malt, 160 kg of cornstarch, 1500 liters of hot water, and 3.0 kg of transglucosidase (“Transglucosidase L” (trade name) manufactured by Amano Enzyme Co., Ltd.) were introduced into a preparation tank, and saccharification was carried out at a temperature in the range of 60°C to 76°C. I did it. This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 8°C using a plate cooler to obtain transglucosidase-treated wort.
 次にトランスグルコシダーゼ処理麦汁にビール酵母を加え、7日間10℃前後で発酵させた後、ビール酵母を除去した。タンクを移し替えて7日間熟成させた後、-1℃付近まで冷却し14日間安定化させた。麦汁発酵液は濾過にて清澄化し、ビールテイスト飲料として容器に充填した。 Next, brewer's yeast was added to the transglucosidase-treated wort, fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days. The fermented wort liquid was clarified by filtration and filled into containers as a beer-taste beverage.
 比較例1と同様にして、得られたビールテイスト飲料の特性を測定した。結果を表2に示す。 The characteristics of the resulting beer-taste beverage were measured in the same manner as in Comparative Example 1. The results are shown in Table 2.
<実施例6>
[ビールテイスト飲料の製造]
 仕込槽にオーツ麦麦芽250kg、大麦麦芽250kg、温水1500リットル及びトランスグルコシダーゼ(天野エンザイム株式会社製「トランスグルコシダーゼL」(商品名))2.0kgを導入し、60℃から76℃の範囲の温度で糖化を行った。この糖化液を濾過槽であるロイターにて濾過し、その後煮沸釜に移して60分間煮沸し、ホップ8kgを添加した。煮沸後、蒸発分の温水を追加し、2500Lとした。ワールプール槽にて熱トルーブを除去した後、プレートクーラーを用いて10℃まで冷却し、トランスグルコシダーゼ処理麦汁を得た。
<Example 6>
[Manufacture of beer-taste beverages]
250 kg of oat malt, 250 kg of barley malt, 1500 liters of hot water, and 2.0 kg of transglucosidase ("Transglucosidase L" (trade name) manufactured by Amano Enzyme Co., Ltd.) were introduced into a preparation tank, and the temperature was maintained in the range of 60°C to 76°C. Saccharification was performed using This saccharified liquid was filtered using a Reuter filter, and then transferred to a boiling pot and boiled for 60 minutes, and 8 kg of hops were added thereto. After boiling, warm water for evaporation was added to make 2500L. After removing the thermotrobe in a whirlpool tank, the mixture was cooled to 10°C using a plate cooler to obtain transglucosidase-treated wort.
 次にトランスグルコシダーゼ処理麦汁にビール酵母を加え、7日間10℃前後で発酵させた後、ビール酵母を除去した。タンクを移し替えて7日間熟成させた後、-1℃付近まで冷却し14日間安定化させて、麦汁発酵液を得た。発酵後の液は濾過にて清澄化し、容器に充填した。 Next, brewer's yeast was added to the transglucosidase-treated wort, fermented at around 10°C for 7 days, and then the brewer's yeast was removed. After changing the tank and aging for 7 days, the mixture was cooled to around -1°C and stabilized for 14 days to obtain a fermented wort liquid. The liquid after fermentation was clarified by filtration and filled into containers.
 比較例1と同様にして、得られたビールテイスト飲料の特性を測定した。結果を表2に示す。 The characteristics of the resulting beer-taste beverage were measured in the same manner as in Comparative Example 1. The results are shown in Table 2.
Figure JPOXMLDOC01-appb-T000002
Figure JPOXMLDOC01-appb-T000002

Claims (10)

  1.  2.0mPa・s以上の粘度を有するビールテイストアルコール飲料。 A beer-taste alcoholic beverage with a viscosity of 2.0 mPa・s or more.
  2.  0.25MPa以下の炭酸ガス圧を有する、請求項1に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to claim 1, having a carbon dioxide pressure of 0.25 MPa or less.
  3.  4容量%以下のアルコール濃度を有する、請求項1又は2に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to claim 1 or 2, having an alcohol concentration of 4% by volume or less.
  4.  容器詰め飲料である、請求項1~3のいずれか一項に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to any one of claims 1 to 3, which is a packaged beverage.
  5.  発酵麦芽飲料である、請求項1~4のいずれか一項に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to any one of claims 1 to 4, which is a fermented malt beverage.
  6.  95%以下の外観最終発酵度を有する、請求項5に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to claim 5, which has an apparent final fermentation degree of 95% or less.
  7.  50~100重量%の麦芽使用比率を有する、請求項5又は6に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to claim 5 or 6, having a malt usage ratio of 50 to 100% by weight.
  8.  糖化に供される原料中のオーツ麦の含有量が15重量%以上である、請求項5~7のいずれか一項に記載のビールテイストアルコール飲料。 The beer-taste alcoholic beverage according to any one of claims 5 to 7, wherein the content of oats in the raw materials subjected to saccharification is 15% by weight or more.
  9.  粘度を2.0mPa・s以上に調節することを含むビールテイストアルコール飲料の製造方法。 A method for producing a beer-taste alcoholic beverage, which includes adjusting the viscosity to 2.0 mPa·s or more.
  10.  粘度を2.0mPa・s以上に調節することを含むビールテイストアルコール飲料の炭酸刺激を増強する方法。 A method for enhancing the carbonation stimulation of a beer-taste alcoholic beverage, which includes adjusting the viscosity to 2.0 mPa·s or more.
PCT/JP2023/028279 2022-09-09 2023-08-02 Beer-taste alcoholic beverage WO2024053297A1 (en)

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Citations (3)

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Publication number Priority date Publication date Assignee Title
JP2008136412A (en) * 2006-12-01 2008-06-19 Asahi Breweries Ltd Method for producing sparkling alcoholic beverage
JP2011072253A (en) * 2009-09-30 2011-04-14 Sapporo Breweries Ltd Sparkling alcoholic beverage and method for producing the same
JP2017118861A (en) * 2015-12-25 2017-07-06 アサヒビール株式会社 Alcoholic taste sparkling beverage

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008136412A (en) * 2006-12-01 2008-06-19 Asahi Breweries Ltd Method for producing sparkling alcoholic beverage
JP2011072253A (en) * 2009-09-30 2011-04-14 Sapporo Breweries Ltd Sparkling alcoholic beverage and method for producing the same
JP2017118861A (en) * 2015-12-25 2017-07-06 アサヒビール株式会社 Alcoholic taste sparkling beverage

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KUMADA, JUN-ICHI: "Foam of beer", KAGAKU TO SEIBUTSU - CHEMISTRY AND BIOLOGY, GAKKAI SHUPPAN SENTA / JAPAN SCIENTIFIC SOCIETIES PRESS, JP, vol. 13, no. 8, 1 January 1975 (1975-01-01), JP , pages 504 - 509, XP009553658, ISSN: 0453-073X, DOI: 10.1271/kagakutoseibutsu1962.13.504 *

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