WO2021256543A1 - Beer-flavored beverage - Google Patents

Beer-flavored beverage Download PDF

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Publication number
WO2021256543A1
WO2021256543A1 PCT/JP2021/023062 JP2021023062W WO2021256543A1 WO 2021256543 A1 WO2021256543 A1 WO 2021256543A1 JP 2021023062 W JP2021023062 W JP 2021023062W WO 2021256543 A1 WO2021256543 A1 WO 2021256543A1
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WO
WIPO (PCT)
Prior art keywords
beer
less
fermentation
mass
taste beverage
Prior art date
Application number
PCT/JP2021/023062
Other languages
French (fr)
Japanese (ja)
Inventor
悠一 加藤
雄悟 早川
智之 岸本
央行 阿部
Original Assignee
サントリーホールディングス株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by サントリーホールディングス株式会社 filed Critical サントリーホールディングス株式会社
Priority to JP2022531915A priority Critical patent/JPWO2021256543A1/ja
Publication of WO2021256543A1 publication Critical patent/WO2021256543A1/en

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Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C11/00Fermentation processes for beer

Definitions

  • the present invention relates to a beer-taste beverage.
  • the present invention provides a beer-taste beverage having a low ethanol content and an excellent fermentation feeling and flavor typical of a beer-taste beverage, and a method for producing the same.
  • the present invention includes the following aspects of the invention.
  • Diacetyl is contained in an amount of less than 0.07 mass ppm
  • ethyl acetate is contained in an amount of 0.07 mass ppm or more
  • ethanol is contained in an amount of 0.4 (v / v)% or more and less than 1.5 (v / v)%.
  • the content of H 2 S is less than 5 mass ppb
  • the beer-taste beverage according to [1] which has a pH of less than 4.0.
  • the amount of yeast added is 40 ⁇ 10 6 cells / mL or more, Fermentation temperature is 0.6-6.0 ° C, How to make beer-taste beverages.
  • the low-alcohol beer is less than 5 ppb by weight of H 2 S, diacetyl less than 0.07 ppm by weight, ethyl acetate 0.07 mass ppm or more, and, ethanol 0.4 (v / v)% or more 1.
  • the beer-taste beverage of one preferred embodiment of the present invention can be a beverage having a low ethanol content and an excellent fermentation feeling and flavor like a beer-taste beverage.
  • the beer-taste beverage of one aspect of the present invention is a beverage having a lower ethanol content than a general beer-taste beverage, and specifically, ethanol is 0.4 (v / v)% or more. It is a beverage contained in an amount of less than 5 (v / v)%.
  • ethanol is 0.4 (v / v)% or more.
  • It is a beverage contained in an amount of less than 5 (v / v)%.
  • the "beer-taste beverage” is not particularly limited as long as it is a beverage exhibiting the flavor of beer.
  • beer-taste beverage examples include alcohol-containing beer-taste beverages, non-alcoholic beer-taste beverages having an alcohol content of less than 1 (v / v)%, and the like. That is, the beer-taste beverage of the present specification includes any carbonated beverage having a beer flavor, unless otherwise specified. Therefore, it is not limited to beverages produced by adding yeast to wheat juice and fermenting, but also esters, higher alcohols (for example, isoamyl acetate, ethyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl caproate), 4 -Includes carbonated beverages to which beer fragrances including vinyl guayacol, linalol, etc.
  • the beer-taste beverage of the present invention may be a malt-based beer-taste beverage that uses malt as a raw material, or a malt-free beer-taste beverage that does not use malt, but a malt-based beer-taste beverage is preferable. Beer-taste beverages using barley malt are more preferred.
  • the beer-taste beverage of the present invention is a fermented beer-taste beverage having a final fermentation degree (LA) of 60% or less, which is obtained through a fermentation step using top-fermenting yeast (Saccharomyces Cerevisiae, etc.) or bottom-fermenting yeast.
  • LA final fermentation degree
  • the beer-taste beverage of the present invention may be a distilled liquor-containing beer-taste beverage containing distilled liquor such as spirits, whiskey, and shochu, and among them, the spirits-containing beer-taste beverage is preferable.
  • the ethanol content of the beer-taste beverage of one aspect of the present invention is less than 1.5 (v / v)%, but is preferably 1.3 (from the viewpoint of making a beer-taste beverage with a further improved fermentation feeling). Less than v / v)%, more preferably less than 1.1 (v / v)%, even more preferably less than 1.0 (v / v)%, less than 0.9 (v / v)%, 0. It may be less than 8 (v / v)%, less than 0.7 (v / v)%, and less than 0.6 (v / v)%.
  • the ethanol content of the beer-taste beverage of one aspect of the present invention is 0.4 (v / v)% or more, preferably 0.41 (v / v)% or more, more preferably 0.42. (V / v)% or more, more preferably 0.43 (v / v)% or more, still more preferably 0.44 (v / v)% or more, still more preferably 0.45 (v / v)%. The above is even more preferably 0.48 (v / v)% or more, and particularly preferably 0.50 (v / v)% or more. In the present specification, the ethanol content is expressed as a volume / volume-based percentage (v / v)%.
  • the ethanol content of the beverage can be measured by any known method, for example, by a vibration densitometer.
  • the ethanol content can be adjusted by adding diluted water or carbonated water, the type of raw material (fermentation, corn glitz, sugar solution, etc.), the amount of raw material, the type of enzyme, the amount of enzyme added, the timing of enzyme addition, and the charging tank.
  • the beer-taste beverage of the present invention contains diacetyl in an amount of less than 0.07% by mass, and the content of diacetyl is preferably 0.068% by mass or less, more preferably 0.065% by mass or less, and 0.063% by mass. It is more preferably ppm or less.
  • the content of diacetyl in the beer-taste beverage of the present invention is 0.060 mass ppm or less, 0.058 mass ppm or less, 0.056 mass ppm or less, 0.053 mass ppm or less, or 0.050 mass ppm or less. May be.
  • the content of diacetyl in the beer-taste beverage of the present invention is not particularly limited at the lower limit, but is 0.001 mass ppm or more, 0.01 mass ppm or more, 0.02 mass ppm or more, 0.03 mass ppm or more. , 0.04 mass ppm or more, or 0.05 mass ppm or more.
  • the content of diacetyl may be adjusted by adding diacetyl to a beer-taste beverage, or may be adjusted by appropriately setting fermentation conditions and the like.
  • the content of diacetyl can be measured by gas chromatography.
  • diacetyl-containing fragrance To adjust the content of diacetyl, add diluted or carbonated water, add diacetyl-containing fragrance, amount of diacetyl-containing fragrance, amount of diacetyl-containing raw material, add diacetyl-containing raw material, type of raw material, amount of raw material, enzyme Type, amount of enzyme added, timing of enzyme addition, set temperature of each temperature range when preparing wheat juice (including during saccharification), holding time and pH, original extract concentration of pre-fermentation liquid, pH of pre-fermentation liquid , Original extract concentration in fermentation process, pH in fermentation process, fermentation conditions (pH, oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure Settings, carbon dioxide concentration, post-fermentation conditions, aging conditions, cooling timing, temperature change timing, etc.) can be appropriately set.
  • the content of H 2 S in the beer-taste beverage of the present invention is less than 5 mass ppb. Since a hot spring odor is emitted when the amount of H 2 S contained in the beverage is large, the content of H 2 S is preferably 4.5 mass ppb or less, more preferably 3.5 mass ppb, still more preferably 3.0 mass ppb or less. , 2.5 mass ppb or less is more preferable, 2.4 mass ppb or less is further preferable, 2.3 mass ppb or less is further preferable, 2.2 mass ppb or less is further preferable, and 2.1 mass ppb or less is further preferable.
  • the beverage of the present invention is particularly preferably free of H 2 S.
  • the content of H 2 S can be determined by gas chromatography.
  • Adjustment of the content of H 2 S, the addition of dilution water or carbonated water, the kind of raw materials, the amount of raw materials, the type of enzyme, the amount of the enzyme, the timing of addition of the enzyme, the preparation of the wort (during saccharification Set temperature, holding time and pH of each temperature range (including), original extract concentration of pre-fermentation liquid, pH of pre-fermentation liquid, original extract concentration in fermentation process, pH in fermentation process, fermentation conditions (pH, oxygen concentration) , Aeration conditions, yeast varieties, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, post-fermentation conditions, aging conditions, cooling timing, temperature change timing, etc.), Beer fermentation conditions and the like can be set as appropriate.
  • H 2 S is to generate yeast. If you want to reduce H 2 S from a beverage or fermented liquid (including during fermentation and when fermentation is completed) after production, use a method of sending gas such as carbon dioxide or nitrogen to the tank, so-called bubbling, to increase the amount of H 2 S. Can be reduced. It can also be reduced by raising the temperature.
  • gas such as carbon dioxide or nitrogen
  • the beer-taste beverage of the present invention has an ethanol content of less than 1.5 (v / v)%, it is necessary to guarantee microorganisms by lowering the pH. Specifically, it is necessary to add an organic acid, which is an acidity-imparting substance, within a range that does not impart an unsuitable acidity to the beverage while keeping the pH below a certain value.
  • an organic acid which is an acidity-imparting substance
  • the pH is lowered to ensure microbial assurance, and an inappropriate acidity is not imparted to the beverage. Is possible.
  • the beer-taste beverage of one aspect of the present invention is a fermented beer-taste beverage having a final fermentation degree (LA) of 60% or less from the viewpoint of further improving the fermented feeling that is typical of beer-taste beverages.
  • LA final fermentation degree
  • the beer-taste beverage of the present invention contains ethyl acetate in an amount of 0.07 mass ppm or more.
  • the content of ethyl acetate is preferably 0.1 mass ppm or more, more preferably 0.15 mass ppm or more, still more preferably 0.3 mass ppm or more, still more preferably 0.5 mass ppm or more, still more preferably.
  • the content of ethyl acetate is preferably 55 mass ppm or less, more preferably 50 mass ppm or less, further preferably 45 mass ppm or less, still more preferably 40 mass ppm or less, still more preferably 37.5 mass ppm or less. , More preferably 35 mass ppm or less, further preferably 32.5 mass ppm or less, particularly preferably 30 mass ppm or less, 25 mass ppm or less, 20 mass ppm or less, 15 mass ppm or less, 10 mass ppm or less, It may be 5 mass ppm or less, 3 mass ppm or less, 2 mass ppm or less, and 1.5 mass ppm or less.
  • the content of ethyl acetate can be measured by gas chromatography.
  • To adjust the content of ethyl acetate add diluted water or carbonated water, add fragrance containing ethyl acetate, amount of fragrance containing ethyl acetate, amount of raw material containing ethyl acetate, add raw material containing ethyl acetate, raw materials containing ethyl acetate.
  • Type amount of ethyl acetate substrate, amount of raw material to be the substrate of ethyl acetate, type of enzyme, amount of enzyme added, timing of addition of enzyme, saccharification time in the preparation tank, protein decomposition time in the preparation tank, preparation
  • the pH in the tank, the pH in the preparation process (the process of producing wheat juice from the addition of malt to the addition of yeast), the amount of acid added for pH adjustment, and the timing of pH adjustment (at the time of preparation, fermentation, fermentation completion) Time, before beer filtration, after beer filtration, etc.), set temperature and holding time of each temperature range when preparing wheat juice (including during saccharification), original extract concentration of pre-fermentation liquid, original extract concentration in fermentation process, Fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, fermentation removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) can be appropriately set. ..
  • the beer-taste beverage according to one aspect of the present invention preferably contains isoamyl acetate in an amount of 0.001 mass ppm or more in order to further improve the fermentation feeling.
  • the content of isoamyl acetate is preferably 0.001% by mass or more, more preferably 0.01% by mass or more, still more preferably 0.02% by mass or more, still more preferably.
  • the content of isoamyl acetate is preferably 25 mass ppm or less, more preferably 20 mass ppm or less, still more preferably 15 mass ppm or less, still more preferably 12.5 mass ppm or less, still more preferably 10 mass ppm or less.
  • the content of isoamyl acetate can be measured by gas chromatography.
  • Type amount of isoamyl acetate substrate, amount of raw material used as substrate for isoamyl acetate, type of enzyme, amount of enzyme added, timing of enzyme addition, saccharification time in the preparation tank, protein decomposition time in the preparation tank, preparation
  • the pH in the tank, the pH in the preparation process (the process of producing wheat juice from the addition of malt to the addition of yeast), the amount of acid added for pH adjustment, and the timing of pH adjustment (at the time of preparation, fermentation, fermentation completion) Time, before beer filtration, after beer filtration, etc.), set temperature and holding time of each temperature range when preparing wheat juice (including during saccharification), original extract concentration of pre-fermentation liquid, original extract concentration in fermentation process, Fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, fermentation removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) can be appropriately set. ..
  • the final fermentation degree (LA) of the beer-taste beverage of the present invention is 60% or less, but the viewpoint of further improving the fermentation feeling like a beer-taste beverage while suppressing the entanol content to less than 1.5 (v / v)%. Therefore, the final fermentation degree (LA) is preferably 15% or more, more preferably 16% or more, further preferably 17% or more, further preferably 18% or more, further preferably 19% or more, and more preferably 20% or more. More preferably, 21% or more is even more preferable, 23% or more is even more preferable, 25% or more is even more preferable, 27% or more is even more preferable, 29% or more is more preferable, 31% or more is even more preferable, and more.
  • the final fermentation degree (LA) of the beer-taste beverage may be 34% or more, 37% or more, or 40% or more, 48% or less, 45% or less, 42% or less, 41% or less, 40% or less. , 39% or less, 38% or less, 37% or less, 36% or less, 35% or less, 34% or less, 33% or less, 32% or less, or 31% or less.
  • final fermentation degree (LA) is the revised BCOJ beer analysis method, Beer Brewing Association, International Technical Committee [Analysis Committee], 2013 supplementary revision (Japan Brewing Society) 8.11 final fermentation degree. It can be measured according to the contents described.
  • the final fermentation degree (LA) value of the beer-taste beverage can be controlled, for example, by adjusting the saccharification conditions of the raw materials, the type and blending amount of the raw materials, and the like. For example, if the saccharification time is lengthened at a temperature at which the saccharifying enzyme exhibits high activity, the decomposition of starch and high molecular weight sugar proceeds, and the final fermentation degree (LA) value increases. By using malt with strong saccharification power, the final fermentation degree (LA) value becomes large. In addition, the final fermentation degree (LA) value is increased by using a sugar solution that is easily assimilated by yeast as a raw material.
  • the lactic acid content of the beverage of the present invention is preferably 1000% by mass or less, more preferably 900% by mass or less, further preferably 700% by mass or less, and further preferably 500% by mass or less. Is even more preferable, 300 mass ppm or less is further preferable, and 200 mass ppm or less is particularly preferable.
  • the lactic acid content of the beverage of the present invention is preferably 50% by mass or more, more preferably 70% by mass or more, still more preferably 100% by mass or more. , 110 mass ppm or more is further preferable, 130 mass ppm or more is further preferable, and 150 mass ppm or more is particularly preferable.
  • the phosphate content of the beverage of the present invention is preferably 1000% by mass or less, more preferably 900% by mass or less, still more preferably 700% by mass or less, in order not to impart an unsuitable acidity to the beer-taste beverage.
  • 500 mass ppm or less is further preferable
  • 300 mass ppm or less is further preferable
  • 200 mass ppm or less is particularly preferable.
  • the phosphate content of the beverage of the present invention is preferably 50% by mass or more, more preferably 70% by mass or more, still more preferably 100% by mass or more.
  • 120 mass ppm or more is further preferable
  • 140 mass ppm or more is further preferable
  • 160 mass ppm or more is particularly preferable.
  • the beer-taste beverage of the present invention preferably contains lactic acid and phosphoric acid.
  • the total content of lactic acid and phosphoric acid is preferably 1700 mass ppm or less, more preferably 1300 mass ppm or less, further preferably 1000 mass ppm or less, and further preferably 700 mass ppm or less, from the viewpoint of imparting a moderate acidity to the beer-taste beverage.
  • 500 mass ppm or less is particularly preferable, and from the viewpoint of lowering the pH of the beverage to obtain a microbial guarantee, 100 mass ppm or more is more preferable, 150 mass ppm or more is further preferable, and 200 mass ppm or more is further more preferable. It is preferable, and 300 mass ppm or more is particularly preferable.
  • the content of lactic acid and phosphoric acid in the beer-taste beverage of the present invention can be controlled, for example, by adjusting the amount of raw materials having a high content of lactic acid, phosphoric acid, lactic acid or phosphoric acid.
  • the beer-taste beverage of the present invention may contain an organic acid other than lactic acid and phosphoric acid.
  • the content of organic acids other than lactic acid and phosphoric acid is less than 100 parts by mass, less than 70 parts by mass, less than 50 parts by mass, less than 20 parts by mass, and 10 parts by mass with respect to 100 parts by mass of the total amount of lactic acid and phosphoric acid. Less than, less than 5 parts by mass, less than 1 part by mass, less than 0.1 parts by mass, or 0 parts by mass.
  • the content of lactic acid and phosphoric acid in the beer-taste beverage of the present invention can be measured by, for example, high performance liquid chromatography.
  • the real extract concentration is preferably 1 (w / w)% or more, more preferably 2 (w / w)% or more, still more preferably 3 (w / w)% or more. , More preferably 4 (w / w)% or more, still more preferably 5 (w / w)% or more, even more preferably 6 (w / w)% or more, still more preferably 6.5 (w / w). )% Or more, more preferably 7 (w / w)% or more, particularly preferably 7.5 (w / w)% or more, 7.7 (w / w)% or more, 7.8 (w / w /).
  • the real extract concentration is preferably 10 (w / w)% or less, more preferably 9.5 (w / w)% or less, still more preferably 9 (w / w / w / w / It is w)% or less, more preferably 8.5 (w / w)% or less, still more preferably 8.4 (w / w)% or less, and even more preferably 8.3 (w / w). )% Or less, and even more preferably 8.2 (w / w)% or less.
  • the real extract concentration can be measured by the method described in the revised BCOJ Beer Analysis Method Beer Brewing Association International Technical Committee [Analysis Committee] ed. can.
  • the real extract concentration can be adjusted by adding diluted water or carbonated water, the type of raw material (wort, corn writ, sugar solution, etc.), wort crushed grain size, wort crushing method (wet crushing, dry crushing, etc.), and wort crushing.
  • Humidity degree of humidity control
  • temperature at wort crushing type of mill used for wort crushing, amount of raw materials, type of enzyme, amount of enzyme added, timing of addition of enzyme, time of enzymatic decomposition, preparation Saccharification time in the tank, protein decomposition time in the preparation tank, pH in the preparation tank, pH in the preparation process (wort production process from wort injection to before wort addition), amount of acid added for pH adjustment , Timing of pH adjustment (at the time of preparation, at the time of fermentation, at the time of completion of fermentation, before beer filtration, after beer filtration, etc.), when preparing wort (including during saccharification), set temperature and holding time of each temperature range, wort Filtering time, temperature during wort filtration, pH during wort filtration, wort recovery amount during wort filtration, amount of sparging water during wort filtration, pH of sparging water during wort filtration, wort Sparging water temperature during filtration, fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number,
  • beverages having a lower ethanol content than general beer-taste beverages tend to have a low content of organic acids obtained by fermentation, so that the refreshing aftertaste of beer tends to be insufficient.
  • the upper limit of the pH of the beer-taste beverage is preferably less than 4.0, more preferably 3.9 or less, still more preferably 3.8 or less, still more preferably 3.7 or less, and particularly preferably 3.6 or less. ..
  • the lower limit of the pH of the beer-taste beverage is preferably 2.7 or more, more preferably 2.8 or more, still more preferably 3.0 or more, still more preferably 3.1 or more, still more preferably 3. It is .2 or more, and particularly preferably 3.3 or more.
  • To adjust the pH add diluted water or carbonated water, the type of raw material (fermentation, corn glitz, sugar solution, etc.), the amount of raw material, the type of enzyme, the amount of enzyme added, the timing of enzyme addition, and the saccharification time in the preparation tank.
  • the addition amount, the number of yeast growths, the timing of removing yeast, the fermentation temperature, the fermentation time, the pressure setting, the carbon dioxide concentration, etc. can be appropriately set.
  • the color of the beer-taste beverage of one aspect of the present invention is not particularly limited, but may be amber or golden like ordinary beer, black like black beer, or colorless and transparent, or a coloring agent. Etc. may be added to give a desired color.
  • the color of the beverage can be discriminated by the naked eye, but it may be defined by the total light transmittance, the chromaticity, or the like.
  • the beer-taste beverage of one aspect of the present invention may be any form in which the beverage is packed in a container, and examples of the container include, for example, bottles, PET bottles, cans, or barrels, but it is particularly easy to carry. From the viewpoint of the above, cans, bottles or PET bottles are preferable.
  • Malt may or may not be used with water as the main raw material for the beer-taste beverage of one aspect of the present invention. Further, hops may be used, and in addition, distilled liquor, sweetener, water-soluble dietary fiber, bitterness or bitterness-imparting agent, antioxidant, flavor, acidity-imparting substance and the like may be used.
  • the malt refers to malt seeds such as barley, wheat, rye, oats, oats, adlay, and oats that have been germinated, dried, and rooted.
  • the variety may be any.
  • barley malt is preferable as the malt to be used.
  • Barley malt is one of the most commonly used malts as a raw material for Japanese beer-taste beverages.
  • barley such as two-row barley and six-row barley, but any of them may be used.
  • colored malt and the like can also be used. When using colored malt, different types of colored malt may be used in appropriate combinations, or one type of colored malt may be used.
  • grains other than malt may be used together with malt.
  • Such grains include, for example, wheat that does not fall under malt (barley, wheat, rye, crow barley, oats, pigeons, starch, etc.), rice (white rice, brown rice, etc.), corn, corn, potatoes, etc. , Beans (soybeans, oats, etc.), buckwheat, sorghum, oats, hie, starch obtained from them, extracts (extracts) thereof and the like.
  • a liquid sugar containing a carbon source and a nitrogen source as an amino acid-containing material such as the above-mentioned grains other than malt.
  • hop form used in one aspect of the present invention examples include pellet hops, powdered hops, hop extracts and the like. Further, as the hop to be used, a processed hop product such as an isodized hop or a reduced hop may be used. The amount of hops added is appropriately adjusted, but is preferably 0.0001 to 1% by mass with respect to the total amount of the beverage.
  • the beer-taste beverage of one aspect of the present invention can contain a pre-prepared distilled liquor as an alcohol component.
  • distilled liquor is not particularly limited, and examples thereof include raw alcohol, spirits, vodka, rum, tequila, gin, and shochu.
  • spirits are alcoholic beverages obtained by using grains such as wheat, rice, buckwheat, and corn as raw materials, saccharifying them with malt or, if necessary, using an enzyme agent, fermenting them with yeast, and then further distilling them. means.
  • the sweetener examples include a commercially available saccharified solution obtained by decomposing starch derived from grains with an acid or an enzyme, commercially available sugars such as starch syrup, sugars of trisaccharides or higher, sugar alcohols, natural sweeteners such as stevia, and artificial sweeteners. Can be mentioned.
  • the form of these saccharides may be a liquid such as a solution or a solid such as a powder.
  • the type of starch raw material grain the method for purifying starch, and the treatment conditions such as hydrolysis with enzymes or acids.
  • a saccharide having an increased proportion of maltose may be used by appropriately setting the conditions for hydrolysis with an enzyme or acid.
  • sucrose, fructose, glucose, maltose, trehalose, maltotriose, and solutions thereof can also be used.
  • artificial sweetener include aspartame, acesulfame potassium (acesulfame K), sucralose and the like.
  • water-soluble dietary fiber examples include indigestible dextrin, polydextrose, guar gum decomposition product, pectin, glucomannan, alginic acid, laminarin, fucoidin, carrageenan, etc. from the viewpoint of versatility such as stability and safety. , Indigestible dextrin or polydextrose is preferred.
  • the bitterness is preferably imparted by hops or the like, but a bitterness agent or a bitterness-imparting agent may be used together with or in place of hops.
  • the bitterness agent or bitterness-imparting agent is not particularly limited, and those used as a bitterness-imparting agent in ordinary beer and effervescent liquor can be used.
  • the antioxidant is not particularly limited, and those used as antioxidants in ordinary beer and low-malt beer can be used, and examples thereof include ascorbic acid, erythorbic acid, and catechin.
  • the fragrance is not particularly limited, and a general beer fragrance can be used. Beer flavors are used for beer-like flavoring.
  • the beer fragrance include esters, higher alcohols and the like, and specific examples thereof include ethyl acetate, isoamyl acetate, n-propanol, isobutanol, ethyl caproate, 4-vinylguaiacol and acetaldehyde.
  • the beer-taste beverage of one aspect of the present invention may contain an acidity-imparting substance other than lactic acid and phosphoric acid.
  • an acidity-imparting substance other than lactic acid and phosphoric acid is not particularly limited as long as it imparts acidity, but for example, citric acid, gluconic acid, malic acid, phytic acid, acetic acid, succinic acid, tartaric acid, pyruvate, and pyro. Glutamic acid, brewed vinegar and salts thereof can be used.
  • the acidity-imparting substances citric acid, tartaric acid, brewed vinegar, and salts thereof are preferable, and brewed vinegar is more preferable.
  • two or more components may be used in combination as the acidity-imparting substance.
  • the content of all acidity-imparting substances including lactic acid and phosphoric acid is such that the acidity-imparting substance has an acidity of 0.01 to 0.4% from the viewpoint of improving the refreshing feeling of the aftertaste. Is preferable.
  • “Acidity” means what is calculated based on the acidity measuring method specified in the Japanese Agricultural Standards (August 8, 2006, Agricultural Water Declaration No. 1127).
  • Carbon dioxide gas contained in the beer-taste beverage of one aspect of the present invention may be added by mixing with carbonated water, or carbon dioxide gas may be added directly to the raw material liquid.
  • the carbon dioxide concentration of the beer-taste beverage of the present invention is preferably 0.30 (w / w)% or more, more preferably 0.35 (w / w)% or more, still more preferably 0.40 (w / w). % Or more, more preferably 0.42 (w / w)% or more, particularly preferably 0.45 (w / w)% or more, and preferably 0.80 (w / w)% or less. More preferably 0.70 (w / w)% or less, still more preferably 0.60 (w / w)% or less, still more preferably 0.57 (w / w) or less, and particularly preferably 0.55 (w). / W)% or less.
  • the carbon dioxide gas concentration is measured by immersing the container containing the target beverage in a water tank at 20 ° C. for 30 minutes or more while shaking it occasionally to adjust the temperature of the beverage to 20 ° C.
  • the measurement can be performed using an apparatus (for example, GVA-500 (manufactured by Kyoto Electronics Industry Co., Ltd.)).
  • the amount of carbon dioxide gas contained in the beer-taste beverage is represented by the carbon dioxide gas pressure of the beverage, but is not particularly limited as long as the effect of the present invention is not impaired.
  • the upper limit of the carbon dioxide gas pressure of the beverage is 5.0kg / cm 2, 4.5kg / cm 2 or 4.0 kg / cm 2
  • the lower limit is 0.20kg / cm 2
  • 0.50kg / It is cm 2 or 1.0 kg / cm 2
  • any of these upper and lower limits may be combined.
  • carbon dioxide gas pressure of the beverage 0.20 kg / cm 2 or more 5.0 kg / cm 2 or less, 0.50 kg / cm 2 or more 4.5 kg / cm 2 or less, or, 1.0 kg / cm 2 or more 4. It may be 0 kg / cm 2 or less.
  • the gas pressure means the gas pressure in the container, except for special cases.
  • the pressure is measured by a method well known to those skilled in the art, for example, after fixing a sample at 20 ° C to a gas internal pressure gauge, the stopcock of the gas internal pressure gauge is opened once to release the gas, the stopcock is closed again, and the gas internal pressure gauge is measured. It can be measured by using a method of swinging and reading the value when the pointer reaches a certain position, or by using a commercially available gas pressure measuring device.
  • additives may be added, if necessary, as long as the effects of the present invention are not impaired.
  • additives include colorants, foam-forming agents, fermentation accelerators, yeast extracts, protein-based substances such as peptide-containing substances, and seasonings such as amino acids.
  • the colorant is used to give a beer-like color to a beverage, and a caramel color or the like can be used.
  • Foam-forming agents are used to form beer-like foam in beverages or to retain foam in beverages, and are plant-extracted saponin-based substances such as soybean saponin and kiraya saponin, and plants such as corn and soybean.
  • Proteins, peptide-containing substances, protein-based substances such as bovine serum albumin, yeast extracts and the like can be appropriately used.
  • the fermentation accelerator is used to promote fermentation by yeast, and for example, yeast extract, bran components such as rice and wheat, vitamins, mineral agents and the like can be used alone or in combination.
  • benzoic acid, benzoate such as sodium benzoate, and dimethyl dicarbonate may be added.
  • the beer-taste beverage of the present invention is a fermented beer-taste beverage including a fermentation step.
  • a method for producing a beer-taste beverage according to one aspect of the present invention will be described.
  • the method for producing a beer-taste beverage of the present invention is not particularly limited, and examples thereof include methods having the following steps (1) and (2).
  • -Step (1) A step of preparing a fermentation raw material liquid by saccharifying a mixture containing various raw materials such as water and malt.
  • -Step (2) A step of adding yeast to the fermentation raw material liquid to perform alcoholic fermentation.
  • one or more of the following steps (3) to (7) may be further provided, if necessary.
  • ⁇ Step (5) H 2 S content step of adjusting the.
  • -Step (6) A step of adjusting the ethanol content.
  • -Step (7) A step of adjusting the lactic acid content and / or the phosphoric acid content.
  • Step (1) is a step of preparing a fermentation raw material liquid by saccharifying a mixture containing various raw materials such as water and malt. It is preferable to use grains as raw materials, and it is more preferable to use wheat and malt.
  • the malt ratio is not particularly limited, but the upper limit is 100% or less, preferably 90% or less, more preferably 80% or less, further preferably 70% or less, still more preferably 60% or less, and the lower limit value. It is preferably adjusted to be preferably 10% or more, more preferably 15% or more, still more preferably 17.5% or more, still more preferably 20% or more, and particularly preferably 22.5% or more.
  • a method for preparing the fermentation raw material liquid a method is obtained in which raw materials such as water and malt are put into a charging kettle or a charging tank, and if necessary, an enzyme such as amylase is added to prepare a mixture, and then saccharification treatment is performed. Can be mentioned. After that, it is preferable that the obtained fermentation raw material liquid is filtered and boiled to remove solids such as coagulated protein in a clarification tank.
  • hops In addition to water and malt, hops, dietary fiber, sweeteners, antioxidants, bitterness-imparting agents, flavors, acidity-imparting substances, pigments and the like may be added as the mixture to be subjected to the saccharification treatment. These may be added before the saccharification treatment, may be added during the saccharification treatment, or may be added after the completion of the saccharification treatment. In addition, these may be added after the alcoholic fermentation of the next step.
  • the mixture to which the raw material is added is heated to saccharify the starch of the raw material.
  • the temperature and time of the saccharification treatment are appropriately adjusted depending on the type of malt used, the malt ratio, raw materials other than water and malt, the type and amount of enzyme used, and the like.
  • the step (2) is a step of adding yeast to the fermentation raw material liquid obtained by the saccharification treatment in the step (1) to perform alcoholic fermentation.
  • the yeast used in this step can be appropriately selected in consideration of the type of fermented beverage to be produced, the desired flavor, fermentation conditions, etc., and may be top-fermented yeast or bottom-fermented yeast. Although good, it is preferable to use bottom fermenting yeast.
  • Yeast may be added to the fermentation raw material solution as it is in a yeast suspension, or a slurry obtained by concentrating yeast by centrifugation or sedimentation may be added to the fermentation raw material solution. Further, after centrifugation, the supernatant may be completely removed before addition.
  • the amount of yeast added to the fermentation feedstock solution can be appropriately set, but is preferably 40 ⁇ 10 6 cells / ml or more, and more preferably 40 ⁇ 10 6 cells / ml to 1 ⁇ 10 8 cells / ml. ..
  • Fermentation conditions for alcoholic fermentation can be set as appropriate, but the ethanol content in the finally obtained beer-taste beverage is 0.4 (v / v)% or more and 1.5 (v / v). % less, diacetyl content of less than 0.07 mass ppm, and it is preferable to set such that the content of H 2 S is less than 5 mass ppb. Since the ethanol content increases in a short time when the fermentation rate is high, it is preferable to ferment at a temperature lower than usual.
  • the fermentation temperature is preferably 21 ° C. or lower, more preferably 15 ° C. or lower, still more preferably 9 ° C. or lower, still more preferably 5 ° C. or lower, still more preferably 4 ° C.
  • the lower limit of the fermentation time is preferably 1 hour or more, more preferably 5 hours or more, still more preferably 15 hours or more, still more preferably 20 hours or more, still more preferably 25 hours or more, still more preferably 30 hours or more. Especially preferably, it is 35 hours or more.
  • the upper limit of the fermentation time is preferably 720 hours or less, more preferably 480 hours or less, still more preferably 360 hours or less, still more preferably 240 hours or less, and particularly preferably 200 hours or less.
  • yeast is removed with a filter or the like.
  • a step of adding additives such as water, a fragrance, an acidity-imparting substance, and a pigment may be included, if necessary. Further, the maltose content, the original extract concentration, the real extract concentration and the like may be adjusted in the step of adding additives such as water, fragrance, acidity-imparting substance and pigment.
  • the order of steps (3), step (4), step (5), step (6) and step (7) is not particularly limited and may be performed from any step.
  • step (3) the diacetyl content in the beverage before preparation is measured, and based on the measured value, a raw material containing diacetyl or diacetyl is added, or water or carbonated water is added to dilute the diacetyl content. It is preferable to make adjustments so as to be within the above-mentioned range. Diacetyl is produced during the fermentation process. Therefore, in the step (2), it is preferable to adjust the diacetyl content by stopping the fermentation in the middle. If the diacetyl content is already within the desired range due to the presence of diacetyl derived from the raw materials in the beverage before preparation, it is not necessary to add diacetyl or dilute by adding water in this step. be.
  • step (4) the ethyl acetate content in the beverage before preparation is measured, and based on the measured value, a raw material containing ethyl acetate or ethyl acetate is added, or water or carbonated water is added to dilute the mixture. It is preferable to make adjustments so that the ethyl acetate content is within the above range. Ethyl acetate is produced in the fermentation process. Therefore, in the step (2), it is preferable to adjust the ethyl acetate content by stopping the fermentation in the middle.
  • the operation of adding ethyl acetate or adding water to dilute the product is performed in this step. Is unnecessary.
  • step (5) the H 2 S content in the beverage before adjustment is measured, and based on the measured value, water or carbonated water is added to the beverage to dilute it, and the above range of the H 2 S content is obtained. It is preferable to make adjustments so as to be. H 2 S is produced in the fermentation process. Therefore, in the step (2), by stopping the fermentation in the middle, it is preferable to adjust the H 2 S content. If the H 2 S content is already within the desired range due to the presence of H 2 S derived from the raw material in the beverage before preparation, an operation such as adding water or carbonated water according to this step to dilute the beverage. Is unnecessary.
  • the ethanol content can be adjusted by controlling the fermentation conditions of the step (2) and adding raw materials containing ethanol such as distilled liquor, but in the step (2), the fermentation is carried out in the middle. It is preferable to adjust the ethanol content by stopping. Further, the ethanol content may be adjusted by adding water, carbonated water or the like to dilute the mixture.
  • step (7) the content of lactic acid and phosphoric acid in the beverage before preparation is measured, and based on the measured value, lactic acid, phosphoric acid, or two acids are added, or water is added to dilute the mixture. It is preferable to adjust the lactic acid content and the phosphoric acid content so as to be in the desired ranges. If the lactic acid content and the phosphoric acid content of the beverage before adjustment are within the desired ranges, adjustment by this step is not necessary.
  • the beer-taste beverage thus obtained is filled in a predetermined container and distributed as a product on the market.
  • the method for bottling beer-taste beverages is not particularly limited, and a bottling method well known to those skilled in the art can be used.
  • the containerging process the beer-taste beverage is filled and sealed in the container.
  • a container of any form and material may be used in the container packing step, and examples of the container are as described above.
  • steps performed in the production of beer-taste beverages well known to those skilled in the art such as a liquor storage step and a filtration step, may be performed.
  • ⁇ Beverage preparation> After the crushed barley malt is put into a charging tank containing 100 L of warm water maintained at 52 ° C., a saccharified solution is obtained so as to have the final fermentation degree (LA) shown in Tables 1 and 2. Adjust the temperature and time. Specifically, the saccharified solution was prepared by gradually raising the temperature while holding the temperature in the temperature range of 45 to 55 ° C. for 5 to 120 minutes and in the temperature range of 60 ° C. to 85 ° C. for 5 to 120 minutes. .. For example, in Example 1, the temperature was maintained at 52 ° C. for 40 minutes, and then at 80 ° C. for 40 minutes to gradually raise the temperature.
  • the content of lactic acid and phosphoric acid in the beverage was added so as to be the amounts shown in Tables 1 and 2.
  • the malt meal was removed by filtration to obtain wort.
  • Liquid sugar and water were added to the obtained wort so as to have the real extract concentrations shown in Tables 1 and 2, and hops were further added and boiled.
  • wort to solid-liquid separation treatment after boiling the resulting clear wort is cooled, by the addition of yeast, ethanol content described in Table 1 and Table 2, H 2 S content, diacetyl content
  • a fermentation solution is prepared by adjusting the fermentation temperature, fermentation time, addition amount and timing of addition of polysaccharide-degrading enzyme so as to have the amount and ethyl acetate content, and the fermentation solution is filtered to obtain a beer test. Beverages were prepared respectively.
  • Example 1 the fermentation temperature 1.0 ° C., subjected to 36 hours fermentation in the number of added yeast (60 ⁇ 10 6 cells / mL ), H 2 S is less than 5 mass ppb, diacetyl is less than 0.07 ppm by weight , It was confirmed that ethyl acetate was 0.07 mass ppm or more, and filtration was carried out.
  • the type of enzyme, the amount and timing of addition, the set temperature of each temperature range when preparing the saccharified solution, the holding time, the fermentation temperature, the fermentation conditions such as the fermentation time, etc. appropriately set, ethanol content described in Table 1 and Table 2, H 2 S content, diacetyl content, ethyl acetate content, the final degree of fermentation (LA), were respectively adjusted such that the real extract concentration, etc. ..
  • Example 1 to 6 and Comparative Examples 1 and 2 shown in Table 1 the beverages were produced under the same conditions except for the contents of lactic acid and phosphoric acid. From these sensory evaluations, it was found that if the contents of lactic acid and phosphoric acid are within a certain range, the acidity unsuitable for beer-taste beverages can be suppressed. In been Examples 7-12 and Comparative Examples 3-7 in Table 2, the content of lactic acid and phosphoric acid are the same, there by fermentation conditions, ethanol, H 2 S, beverages with varying content of diacetyl, etc. rice field.

Abstract

Provided is a beer-flavored beverage that contains less than 0.07 ppm by mass diacetyl, at least 0.07 ppm by mass ethyl acetate, and at least 0.4 (v/v)% but less than 1.5 (v/v)% ethanol; has a H2S content of less than 5 ppb by mass; and has a final degree of fermentation (LA) of at most 60%.

Description

ビールテイスト飲料Beer taste beverage
 本発明は、ビールテイスト飲料に関する。 The present invention relates to a beer-taste beverage.
 近年の消費者の健康志向から、アルコール濃度を低減させたローアルコールビールに対する需要が拡大している。しかし、ローアルコールビールは、通常のビールよりも全体的に味が薄く感じられる傾向にあり、厚みがない平たんな味になりやすい。
 一般的に、香味で厚みを付与するために、添加剤を配合することによって香味を改善する方法が知られている。たとえば、特許文献1(特開2019-208453号公報)には、ヒドロキシ酸エステル類を配合し、かつヒドロキシ酸エステル類の濃度(質量)に対する甘味度の比を特定の範囲内に調整することにより、エキス分を増大させることなく、のどへのひっかかりを増大させられるノンアルコールビールが記載されている。
 一般的なビールの製造における発酵工程では、糖やアミノ酸などが酵母に代謝され、エタノールやエステル類、高級アルコール類などが酵母によって産生され、これらの成分がビールらしい風味を付与する。しかし、一般的に、エタノール含有量が0.00%のノンアルコールビールテイスト飲料は発酵工程を用いずに製造されるため、エステル類、高級アルコール等の香料を添加して発酵感を付与することがあるが、このような香料の添加だけではビールらしい発酵感を再現することは難しい。
Due to the health consciousness of consumers in recent years, the demand for low-alcohol beer with reduced alcohol concentration is increasing. However, low-alcohol beer tends to have a lighter taste as a whole than ordinary beer, and tends to have a flat taste with no thickness.
Generally, there is known a method of improving the flavor by adding an additive in order to impart thickness to the flavor. For example, in Patent Document 1 (Japanese Unexamined Patent Publication No. 2019-208453), hydroxy acid esters are blended, and the ratio of sweetness to the concentration (mass) of hydroxy acid esters is adjusted within a specific range. , Non-alcoholic beer that can increase the catch on the throat without increasing the extract content is described.
In the fermentation process in general beer production, sugars and amino acids are metabolized to yeast, and ethanol, esters, higher alcohols and the like are produced by yeast, and these components impart a beer-like flavor. However, in general, non-alcoholic beer-taste beverages with an ethanol content of 0.00% are produced without using a fermentation process, so flavorings such as esters and higher alcohols should be added to give a fermented feeling. However, it is difficult to reproduce the fermented feeling like beer only by adding such a fragrance.
特開2019-208453号公報Japanese Unexamined Patent Publication No. 2019-208453
 本発明は、エタノール含有量が低く、ビールテイスト飲料らしい発酵感および風味が優れたビールテイスト飲料およびその製造方法を提供する。 The present invention provides a beer-taste beverage having a low ethanol content and an excellent fermentation feeling and flavor typical of a beer-taste beverage, and a method for producing the same.
 本発明には、以下の態様の発明が含まれる。
[1]
 ジアセチルを0.07質量ppm未満、酢酸エチルを0.07質量ppm以上、および、エタノールを0.4(v/v)%以上1.5(v/v)%未満の量で含有し、
 HSの含有量が5質量ppb未満であり、
 最終発酵度(LA)が60%以下である、ビールテイスト飲料。
[2]
 pHが4.0未満である、[1]に記載のビールテイスト飲料。
[3]
 乳酸を50~1000質量ppm、または、リン酸を50~1000ppm含有する、[1]または[2]に記載のビールテイスト飲料。
[4]
 リアルエキス濃度が1~10(w/w)%である、[1]~[4]のいずれかに記載のビールテイスト飲料。
[5]
 麦芽と水を含む原材料から麦汁を製造する工程、
 麦汁に酵母を添加して発酵させる工程、および
 発酵を途中で停止させる工程を有し、
 酵母の添加量が40×10cells/mL以上であり、
 発酵温度が0.6~6.0℃である、
 ビールテイスト飲料の製造方法。
[6]
 発酵工程の発酵時間が25時間以上である、[5]に記載の製造方法
[7]
 前記ビールテイスト飲料の最終発酵度(LA)が50%以下である、[5]または[6]に記載の製造方法。
[8]
 ホップを添加する工程をさらに含む、[5]~[7]のいずれかに記載の製造方法。
[9]
 醸造酢を添加する工程をさらに含む、[5]~[8]のいずれかに記載の製造方法。
[10]
 前記酵母が下面発酵酵母である、[5]~[9]のいずれかに記載の製造方法。
[11]
 前記ビールテイスト飲料が、HSを5質量ppb未満、ジアセチルを0.07質量ppm未満、酢酸エチルを0.07質量ppm以上、および、エタノールを0.4(v/v)%以上1.5(v/v)%未満の量で含有する、[5]~[10]のいずれかに記載の製造方法。
[12]
 [5]~[11]のいずれかに記載の製造方法で製造されたビールテイスト飲料。
[13]
 [5]~[11]のいずれかに記載の製造方法で製造されたビールテイスト飲料であって、[1]~[5]のいずれかに記載のビールテイスト飲料。
The present invention includes the following aspects of the invention.
[1]
Diacetyl is contained in an amount of less than 0.07 mass ppm, ethyl acetate is contained in an amount of 0.07 mass ppm or more, and ethanol is contained in an amount of 0.4 (v / v)% or more and less than 1.5 (v / v)%.
The content of H 2 S is less than 5 mass ppb,
A beer-taste beverage with a final fermentation degree (LA) of 60% or less.
[2]
The beer-taste beverage according to [1], which has a pH of less than 4.0.
[3]
The beer-taste beverage according to [1] or [2], which contains 50 to 1000 ppm by mass of lactic acid or 50 to 1000 ppm of phosphoric acid.
[4]
The beer-taste beverage according to any one of [1] to [4], wherein the real extract concentration is 1 to 10 (w / w)%.
[5]
The process of producing wort from raw materials containing malt and water,
It has a process of adding yeast to wort and fermenting it, and a process of stopping fermentation in the middle.
The amount of yeast added is 40 × 10 6 cells / mL or more,
Fermentation temperature is 0.6-6.0 ° C,
How to make beer-taste beverages.
[6]
The production method [7] according to [5], wherein the fermentation time in the fermentation step is 25 hours or more.
The production method according to [5] or [6], wherein the final fermentation degree (LA) of the beer-taste beverage is 50% or less.
[8]
The production method according to any one of [5] to [7], further comprising a step of adding hops.
[9]
The production method according to any one of [5] to [8], further comprising a step of adding brewed vinegar.
[10]
The production method according to any one of [5] to [9], wherein the yeast is a bottom fermentation yeast.
[11]
The low-alcohol beer is less than 5 ppb by weight of H 2 S, diacetyl less than 0.07 ppm by weight, ethyl acetate 0.07 mass ppm or more, and, ethanol 0.4 (v / v)% or more 1. The production method according to any one of [5] to [10], which is contained in an amount of less than 5 (v / v)%.
[12]
A beer-taste beverage produced by the production method according to any one of [5] to [11].
[13]
The beer-taste beverage produced by the production method according to any one of [5] to [11] and according to any one of [1] to [5].
 本発明の好適な一態様のビールテイスト飲料は、エタノール含有量が低く、ビールテイスト飲料らしい発酵感および風味が優れた飲料となり得る。 The beer-taste beverage of one preferred embodiment of the present invention can be a beverage having a low ethanol content and an excellent fermentation feeling and flavor like a beer-taste beverage.
1.ビールテイスト飲料
 本発明の一態様のビールテイスト飲料は、一般的なビールテイスト飲料に比べてエタノール含有量が少ない飲料であり、具体的にはエタノールを0.4(v/v)%以上1.5(v/v)%未満の量で含有する飲料である。これによって、エタノールを含まないノンアルコールビールテイスト飲料に比べて、ビールテイスト飲料らしい発酵感に優れると共に、風味が優れた飲料を提供できる。
 本明細書において、「ビールテイスト飲料」はビールの風味を呈する飲料であれば特に限定されない。ビールテイスト飲料の種類としては、たとえば、アルコール含有のビールテイスト飲料、アルコール度数が1(v/v)%未満のノンアルコールビールテイスト飲料等も含まれる。つまり、本明細書のビールテイスト飲料は、特に断わりがない場合、ビール風味を有するいずれの炭酸飲料をも包含する。したがって、麦汁に酵母を添加して発酵させて製造される飲料に限定されず、エステル、高級アルコール(たとえば、酢酸イソアミル、酢酸エチル、n-プロパノール、イソブタノール、アセトアルデヒド、カプロン酸エチル)、4-ビニルグアイアコール、リナロール等を含むビール香料が添加された炭酸飲料も包含する。
 本発明のビールテイスト飲料は、原料として麦芽を用いた麦芽使用ビールテイスト飲料であってもよく、麦芽を用いない麦芽不使用ビールテイスト飲料であってもよいが、麦芽使用ビールテイスト飲料が好ましく、大麦麦芽使用ビールテイスト飲料がより好ましい。
 本発明のビールテイスト飲料は、上面発酵酵母(サッカロマイセス・セレビシエ(Saccharomyces Cerevisiae)等)や下面発酵酵母を用いて発酵工程を経て得られる、最終発酵度(LA)が60%以下の発酵ビールテイスト飲料であり、発酵麦芽ビールテイスト飲料がより好ましく、発酵大麦麦芽ビールテイスト飲料がさらに好ましい。
 また、本発明のビールテイスト飲料は、スピリッツ、ウイスキー、焼酎などの蒸留酒を含有する、蒸留酒含有ビールテイスト飲料であってもよく、その中では、スピリッツ含有ビールテイスト飲料が好ましい。
1. 1. Beer-taste beverage The beer-taste beverage of one aspect of the present invention is a beverage having a lower ethanol content than a general beer-taste beverage, and specifically, ethanol is 0.4 (v / v)% or more. It is a beverage contained in an amount of less than 5 (v / v)%. As a result, it is possible to provide a beverage having an excellent fermented feeling and a good flavor, which is typical of a beer-taste beverage, as compared with a non-alcoholic beer-taste beverage that does not contain ethanol.
In the present specification, the "beer-taste beverage" is not particularly limited as long as it is a beverage exhibiting the flavor of beer. Examples of the type of beer-taste beverage include alcohol-containing beer-taste beverages, non-alcoholic beer-taste beverages having an alcohol content of less than 1 (v / v)%, and the like. That is, the beer-taste beverage of the present specification includes any carbonated beverage having a beer flavor, unless otherwise specified. Therefore, it is not limited to beverages produced by adding yeast to wheat juice and fermenting, but also esters, higher alcohols (for example, isoamyl acetate, ethyl acetate, n-propanol, isobutanol, acetaldehyde, ethyl caproate), 4 -Includes carbonated beverages to which beer fragrances including vinyl guayacol, linalol, etc. have been added.
The beer-taste beverage of the present invention may be a malt-based beer-taste beverage that uses malt as a raw material, or a malt-free beer-taste beverage that does not use malt, but a malt-based beer-taste beverage is preferable. Beer-taste beverages using barley malt are more preferred.
The beer-taste beverage of the present invention is a fermented beer-taste beverage having a final fermentation degree (LA) of 60% or less, which is obtained through a fermentation step using top-fermenting yeast (Saccharomyces Cerevisiae, etc.) or bottom-fermenting yeast. Therefore, fermented malt beer-taste beverages are more preferable, and fermented barley malt beer-taste beverages are even more preferable.
Further, the beer-taste beverage of the present invention may be a distilled liquor-containing beer-taste beverage containing distilled liquor such as spirits, whiskey, and shochu, and among them, the spirits-containing beer-taste beverage is preferable.
 本発明の一態様のビールテイスト飲料のエタノール含有量は、1.5(v/v)%未満であるが、発酵感をより向上させたビールテイスト飲料とする観点から、好ましくは1.3(v/v)%未満、より好ましくは1.1(v/v)%未満、さらに好ましくは1.0(v/v)%未満であり、0.9(v/v)%未満、0.8(v/v)%未満、0.7(v/v)%未満、0.6(v/v)%未満であってもよい。
 また、本発明の一態様のビールテイスト飲料のエタノール含有量は、0.4(v/v)%以上であるが、好ましくは0.41(v/v)%以上、より好ましくは0.42(v/v)%以上、さらに好ましくは0.43(v/v)%以上、よりさらに好ましくは0.44(v/v)%以上、よりさらに好ましくは0.45(v/v)%以上、よりさらに好ましくは0.48(v/v)%以上、特に好ましくは0.50(v/v)%以上である。
 本明細書において、エタノール含有量は、体積/体積基準の百分率(v/v)%で示されるものとする。また、飲料のエタノール含有量は、公知のいずれの方法によっても測定することができるが、たとえば、振動式密度計によって測定することができる。
 エタノール含有量の調整は、希釈水または炭酸水の添加、原材料(麦芽、コーングリッツ、糖液等)の種類、原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、仕込槽で糖化時間、仕込槽でのタンパク分解時間、仕込槽でのpH、仕込工程(麦芽投入から酵母添加前での麦汁製造工程)でのpH、pH調整の際に使用する酸の添加量、pH調整のタイミング(仕込時、発酵時、発酵完了時、ビール濾過前、ビール濾過後など)、麦汁を調製する際(糖化時含む)の各温度領域の設定温度及び保持時間、発酵前液のオリジナルエキス濃度、発酵工程でのオリジナルエキス濃度、発酵条件(酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度等)、スピリッツや醸造アルコールなどの添加等を適宜設定して行うことができる。
The ethanol content of the beer-taste beverage of one aspect of the present invention is less than 1.5 (v / v)%, but is preferably 1.3 (from the viewpoint of making a beer-taste beverage with a further improved fermentation feeling). Less than v / v)%, more preferably less than 1.1 (v / v)%, even more preferably less than 1.0 (v / v)%, less than 0.9 (v / v)%, 0. It may be less than 8 (v / v)%, less than 0.7 (v / v)%, and less than 0.6 (v / v)%.
The ethanol content of the beer-taste beverage of one aspect of the present invention is 0.4 (v / v)% or more, preferably 0.41 (v / v)% or more, more preferably 0.42. (V / v)% or more, more preferably 0.43 (v / v)% or more, still more preferably 0.44 (v / v)% or more, still more preferably 0.45 (v / v)%. The above is even more preferably 0.48 (v / v)% or more, and particularly preferably 0.50 (v / v)% or more.
In the present specification, the ethanol content is expressed as a volume / volume-based percentage (v / v)%. The ethanol content of the beverage can be measured by any known method, for example, by a vibration densitometer.
The ethanol content can be adjusted by adding diluted water or carbonated water, the type of raw material (fermentation, corn glitz, sugar solution, etc.), the amount of raw material, the type of enzyme, the amount of enzyme added, the timing of enzyme addition, and the charging tank. Saccharification time, protein decomposition time in the preparation tank, pH in the preparation tank, pH in the preparation process (beer production process from malt addition to before yeast addition), amount of acid added for pH adjustment, pH Adjustment timing (during preparation, fermentation, completion of fermentation, before beer filtration, after beer filtration, etc.), set temperature and holding time of each temperature range when preparing wheat juice (including during saccharification), pre-fermentation liquid Original extract concentration, original extract concentration in fermentation process, fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration Etc.), addition of spirits, brewed alcohol, etc. can be appropriately set.
 本発明のビールテイスト飲料は、ジアセチルを0.07質量ppm未満で含有するが、ジアセチルの含有量は、0.068質量ppm以下が好ましく、0.065質量ppm以下がより好ましく、0.063質量ppm以下がさらに好ましい。また、本発明のビールテイスト飲料におけるジアセチルの含有量は、0.060質量ppm以下、0.058質量ppm以下、0.056質量ppm以下、0.053質量ppm以下、または0.050質量ppm以下であってもよい。
 また、本発明のビールテイスト飲料におけるジアセチルの含有量は、下限の制限は特にないが、0.001質量ppm以上、0.01質量ppm以上、0.02質量ppm以上、0.03質量ppm以上、0.04質量ppm以上、または0.05質量ppm以上であってもよい。
 なお、ジアセチルの含有量は、ビールテイスト飲料に、ジアセチルを添加して調整してもよく、発酵条件等を適宜設定の上で調整してもよい。
 ジアセチルの含有量はガスクロマトグラフィーによって測定できる。
 ジアセチルの含有量の調整は、希釈水または炭酸水の添加、ジアセチル含有香料の添加、ジアセチル含有香料の量、ジアセチル含有原料の量、ジアセチル含有原料の添加、原材料の種類、原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、麦汁を調製する際(糖化時含む)の各温度領域の設定温度、保持時間及びpH、発酵前液のオリジナルエキス濃度、発酵前液のpH、発酵工程でのオリジナルエキス濃度、発酵工程でのpH、発酵条件(pH、酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度、後発酵条件、熟成条件、冷却タイミング、温度変更タイミング等)等を適宜設定して行うことができる。
The beer-taste beverage of the present invention contains diacetyl in an amount of less than 0.07% by mass, and the content of diacetyl is preferably 0.068% by mass or less, more preferably 0.065% by mass or less, and 0.063% by mass. It is more preferably ppm or less. The content of diacetyl in the beer-taste beverage of the present invention is 0.060 mass ppm or less, 0.058 mass ppm or less, 0.056 mass ppm or less, 0.053 mass ppm or less, or 0.050 mass ppm or less. May be.
The content of diacetyl in the beer-taste beverage of the present invention is not particularly limited at the lower limit, but is 0.001 mass ppm or more, 0.01 mass ppm or more, 0.02 mass ppm or more, 0.03 mass ppm or more. , 0.04 mass ppm or more, or 0.05 mass ppm or more.
The content of diacetyl may be adjusted by adding diacetyl to a beer-taste beverage, or may be adjusted by appropriately setting fermentation conditions and the like.
The content of diacetyl can be measured by gas chromatography.
To adjust the content of diacetyl, add diluted or carbonated water, add diacetyl-containing fragrance, amount of diacetyl-containing fragrance, amount of diacetyl-containing raw material, add diacetyl-containing raw material, type of raw material, amount of raw material, enzyme Type, amount of enzyme added, timing of enzyme addition, set temperature of each temperature range when preparing wheat juice (including during saccharification), holding time and pH, original extract concentration of pre-fermentation liquid, pH of pre-fermentation liquid , Original extract concentration in fermentation process, pH in fermentation process, fermentation conditions (pH, oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure Settings, carbon dioxide concentration, post-fermentation conditions, aging conditions, cooling timing, temperature change timing, etc.) can be appropriately set.
 本発明のビールテイスト飲料のHSの含有量は5質量ppb未満である。飲料に含まれるHSが多いと温泉臭がするので、HSの含有量は4.5質量ppb以下が好ましく、3.5質量ppbがより好ましく、3.0質量ppb以下がさらに好ましく、2.5質量ppb以下がさらに好ましく、2.4質量ppb以下がさらに好ましく、2.3質量ppb以下がさらに好ましく、2.2質量ppb以下がさらに好ましく、2.1質量ppb以下がさらに好ましく、2.0質量ppb以下がさらに好ましく、1.9質量ppb以下がさらに好ましく、1.8質量ppb以下がさらに好ましく、1.7質量ppb以下がさらに好ましく、1.6質量ppb以下がさらに好ましく、1.5質量ppb以下がさらに好ましく、1.4質量ppb以下がさらに好ましく、1.3質量ppb以下がさらに好ましく、1.2質量ppb以下がさらに好ましく、1.1質量ppb以下がさらに好ましく、1.0質量ppb以下がよりさらに好ましい。また、本発明の飲料はHSを含まないことが特に好ましい。
 本明細書において、HSの含有量はガスクロマトグラフィーによって測定することができる。
 HSの含有量の調整は、希釈水または炭酸水の添加、原材料の種類、原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、麦汁を調製する際(糖化時含む)の各温度領域の設定温度、保持時間及びpH、発酵前液のオリジナルエキス濃度、発酵前液のpH、発酵工程でのオリジナルエキス濃度、発酵工程でのpH、発酵条件(pH、酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度、後発酵条件、熟成条件、冷却タイミング、温度変更タイミング等)、ビール濾過条件等を適宜設定して行うことができる。HSは酵母が生成するものである。生成した後に、飲料あるいは発酵液(発酵中、発酵完了時含む)からHSを減らしたい場合は二酸化炭素や窒素などの気体をタンクへ送り込む方法、いわゆるバブリングという方法でHSの量を減らすことができる。また、温度を上げることでも減らすことができる。
The content of H 2 S in the beer-taste beverage of the present invention is less than 5 mass ppb. Since a hot spring odor is emitted when the amount of H 2 S contained in the beverage is large, the content of H 2 S is preferably 4.5 mass ppb or less, more preferably 3.5 mass ppb, still more preferably 3.0 mass ppb or less. , 2.5 mass ppb or less is more preferable, 2.4 mass ppb or less is further preferable, 2.3 mass ppb or less is further preferable, 2.2 mass ppb or less is further preferable, and 2.1 mass ppb or less is further preferable. , 2.0 mass ppb or less is more preferable, 1.9 mass ppb or less is further preferable, 1.8 mass ppb or less is further preferable, 1.7 mass ppb or less is further preferable, and 1.6 mass ppb or less is further preferable. , 1.5 mass ppb or less is further preferable, 1.4 mass ppb or less is further preferable, 1.3 mass ppb or less is further preferable, 1.2 mass ppb or less is further preferable, and 1.1 mass ppb or less is further preferable. , 1.0 mass ppb or less is even more preferable. Further, the beverage of the present invention is particularly preferably free of H 2 S.
In the present specification, the content of H 2 S can be determined by gas chromatography.
Adjustment of the content of H 2 S, the addition of dilution water or carbonated water, the kind of raw materials, the amount of raw materials, the type of enzyme, the amount of the enzyme, the timing of addition of the enzyme, the preparation of the wort (during saccharification Set temperature, holding time and pH of each temperature range (including), original extract concentration of pre-fermentation liquid, pH of pre-fermentation liquid, original extract concentration in fermentation process, pH in fermentation process, fermentation conditions (pH, oxygen concentration) , Aeration conditions, yeast varieties, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, post-fermentation conditions, aging conditions, cooling timing, temperature change timing, etc.), Beer fermentation conditions and the like can be set as appropriate. H 2 S is to generate yeast. If you want to reduce H 2 S from a beverage or fermented liquid (including during fermentation and when fermentation is completed) after production, use a method of sending gas such as carbon dioxide or nitrogen to the tank, so-called bubbling, to increase the amount of H 2 S. Can be reduced. It can also be reduced by raising the temperature.
 本発明のビールテイスト飲料は、エタノールの含有量が1.5(v/v)%未満であるため、pHを下げることによる微生物保証が必要になる。具体的には、pHを一定の値以下としながら、不適な酸味を飲料に付与しない範囲で酸味付与物質である有機酸を添加する必要がある。
 本発明のビールテイスト飲料においては、乳酸を50~1000質量ppm、または、リン酸を50~1000質量ppm含むことによって、pHを下げて微生物保証を確保しながら、不適な酸味を飲料に付与しないことが可能となる。
Since the beer-taste beverage of the present invention has an ethanol content of less than 1.5 (v / v)%, it is necessary to guarantee microorganisms by lowering the pH. Specifically, it is necessary to add an organic acid, which is an acidity-imparting substance, within a range that does not impart an unsuitable acidity to the beverage while keeping the pH below a certain value.
In the beer-taste beverage of the present invention, by containing 50 to 1000 mass ppm of lactic acid or 50 to 1000 mass ppm of phosphoric acid, the pH is lowered to ensure microbial assurance, and an inappropriate acidity is not imparted to the beverage. Is possible.
 本発明の一態様のビールテイスト飲料は、ビールテイスト飲料らしい発酵感をより向上させる観点から、最終発酵度(LA)が60%以下の発酵ビールテイスト飲料である。 酵母を用いた発酵工程を経ると、酢酸エチルや酢酸イソアミルといった発酵感をより向上させ得る香気成分が生じる。そのため、本発明のビールテイスト飲料は、酢酸エチルを0.07質量ppm以上の量で含有する。
 酢酸エチルの含有量は、好ましくは0.1質量ppm以上、より好ましくは0.15質量ppm以上、さらに好ましくは0.3質量ppm以上、よりさらに好ましくは0.5質量ppm以上、よりさらに好ましくは0.6質量ppm以上、よりさらに好ましくは0.7質量ppm以上、よりさらに好ましくは0.8質量ppm以上、よりさらに好ましくは0.9質量ppm以上、特に好ましくは1.0質量ppm以上である。また、酢酸エチルの含有量は、好ましくは55質量ppm以下、より好ましくは50質量ppm以下、さらに好ましくは45質量ppm以下、よりさらに好ましくは40質量ppm以下、さらに好ましくは37.5質量ppm以下、よりさらに好ましくは35質量ppm以下、さらに好ましくは32.5質量ppm以下、特に好ましくは30質量ppm以下であり、25質量ppm以下、20質量ppm以下、15質量ppm以下、10質量ppm以下、5質量ppm以下、3質量ppm以下、2質量ppm以下、1.5質量ppm以下であってもよい。
 酢酸エチルの含有量はガスクロマトグラフィーによって測定することができる。
 酢酸エチルの含有量の調整は、希釈水または炭酸水の添加、酢酸エチル含有香料の添加、酢酸エチル含有香料の量、酢酸エチル含有原料の量、酢酸エチル含有原料の添加、酢酸エチルを含む原材料の種類、酢酸エチルの基質の量、酢酸エチルの基質となる原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、仕込槽で糖化時間、仕込槽でのタンパク分解時間、仕込槽でのpH、仕込工程(麦芽投入から酵母添加前での麦汁製造工程)でのpH、pH調整の際に使用する酸の添加量、pH調整のタイミング(仕込時、発酵時、発酵完了時、ビール濾過前、ビール濾過後など)、麦汁を調製する際(糖化時含む)の各温度領域の設定温度及び保持時間、発酵前液のオリジナルエキス濃度、発酵工程でのオリジナルエキス濃度、発酵条件(酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度等)等を適宜設定して行うことができる。
The beer-taste beverage of one aspect of the present invention is a fermented beer-taste beverage having a final fermentation degree (LA) of 60% or less from the viewpoint of further improving the fermented feeling that is typical of beer-taste beverages. Through the fermentation process using yeast, aroma components such as ethyl acetate and isoamyl acetate that can further improve the fermentation feeling are produced. Therefore, the beer-taste beverage of the present invention contains ethyl acetate in an amount of 0.07 mass ppm or more.
The content of ethyl acetate is preferably 0.1 mass ppm or more, more preferably 0.15 mass ppm or more, still more preferably 0.3 mass ppm or more, still more preferably 0.5 mass ppm or more, still more preferably. Is 0.6 mass ppm or more, more preferably 0.7 mass ppm or more, still more preferably 0.8 mass ppm or more, still more preferably 0.9 mass ppm or more, and particularly preferably 1.0 mass ppm or more. Is. The content of ethyl acetate is preferably 55 mass ppm or less, more preferably 50 mass ppm or less, further preferably 45 mass ppm or less, still more preferably 40 mass ppm or less, still more preferably 37.5 mass ppm or less. , More preferably 35 mass ppm or less, further preferably 32.5 mass ppm or less, particularly preferably 30 mass ppm or less, 25 mass ppm or less, 20 mass ppm or less, 15 mass ppm or less, 10 mass ppm or less, It may be 5 mass ppm or less, 3 mass ppm or less, 2 mass ppm or less, and 1.5 mass ppm or less.
The content of ethyl acetate can be measured by gas chromatography.
To adjust the content of ethyl acetate, add diluted water or carbonated water, add fragrance containing ethyl acetate, amount of fragrance containing ethyl acetate, amount of raw material containing ethyl acetate, add raw material containing ethyl acetate, raw materials containing ethyl acetate. Type, amount of ethyl acetate substrate, amount of raw material to be the substrate of ethyl acetate, type of enzyme, amount of enzyme added, timing of addition of enzyme, saccharification time in the preparation tank, protein decomposition time in the preparation tank, preparation The pH in the tank, the pH in the preparation process (the process of producing wheat juice from the addition of malt to the addition of yeast), the amount of acid added for pH adjustment, and the timing of pH adjustment (at the time of preparation, fermentation, fermentation completion) Time, before beer filtration, after beer filtration, etc.), set temperature and holding time of each temperature range when preparing wheat juice (including during saccharification), original extract concentration of pre-fermentation liquid, original extract concentration in fermentation process, Fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, fermentation removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) can be appropriately set. ..
 また、本発明の一態様のビールテイスト飲料は、発酵感をより向上させるために酢酸イソアミルを0.001質量ppm以上の量で含有することが好ましい。
 本発明の一態様のビールテイスト飲料において、酢酸イソアミルの含有量は、好ましくは0.001質量ppm以上、より好ましくは0.01質量ppm以上、さらに好ましくは0.02質量ppm以上、よりさらに好ましくは0.03質量ppm以上、よりさらに好ましくは0.04質量ppm以上、よりさらに好ましくは0.05質量ppm以上、よりさらに好ましくは0.06質量ppm以上、よりさらに好ましくは0.07質量ppm以上、よりさらに好ましくは0.08質量ppm以上、よりさらに好ましくは0.09質量ppm以上、特に好ましくは0.10質量ppm以上である。
 また、酢酸イソアミルの含有量は、好ましくは25質量ppm以下、より好ましくは20質量ppm以下、さらに好ましくは15質量ppm以下、よりさらに好ましくは12.5質量ppm以下、さらに好ましくは10質量ppm以下、よりさらに好ましくは9質量ppm以下、さらに好ましくは8質量ppm以下、よりさらに好ましくは7質量ppm以下、さらに好ましくは6質量ppm以下、特に好ましくは5質量ppm以下であり、4質量ppm以下、3質量ppm以下、2質量ppm以下、1質量ppm以下、0.70質量ppm以下、0.50質量ppm以下、0.30質量ppm以下、0.20質量ppm以下であってもよい。
 酢酸イソアミルの含有量はガスクロマトグラフィーによって測定することができる。
 酢酸イソアミルの含有量の調整は、希釈水または炭酸水の添加、酢酸イソアミル含有香料の添加、酢酸イソアミル含有香料の量、酢酸イソアミル含有原料の量、酢酸イソアミル含有原料の添加、酢酸イソアミルを含む原材料の種類、酢酸イソアミルの基質の量、酢酸イソアミルの基質となる原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、仕込槽で糖化時間、仕込槽でのタンパク分解時間、仕込槽でのpH、仕込工程(麦芽投入から酵母添加前での麦汁製造工程)でのpH、pH調整の際に使用する酸の添加量、pH調整のタイミング(仕込時、発酵時、発酵完了時、ビール濾過前、ビール濾過後など)、麦汁を調製する際(糖化時含む)の各温度領域の設定温度及び保持時間、発酵前液のオリジナルエキス濃度、発酵工程でのオリジナルエキス濃度、発酵条件(酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度等)等を適宜設定して行うことができる。
Further, the beer-taste beverage according to one aspect of the present invention preferably contains isoamyl acetate in an amount of 0.001 mass ppm or more in order to further improve the fermentation feeling.
In the beer-taste beverage of one aspect of the present invention, the content of isoamyl acetate is preferably 0.001% by mass or more, more preferably 0.01% by mass or more, still more preferably 0.02% by mass or more, still more preferably. Is 0.03 mass ppm or more, more preferably 0.04 mass ppm or more, still more preferably 0.05 mass ppm or more, still more preferably 0.06 mass ppm or more, still more preferably 0.07 mass ppm or more. As described above, it is more preferably 0.08 mass ppm or more, still more preferably 0.09 mass ppm or more, and particularly preferably 0.10 mass ppm or more.
The content of isoamyl acetate is preferably 25 mass ppm or less, more preferably 20 mass ppm or less, still more preferably 15 mass ppm or less, still more preferably 12.5 mass ppm or less, still more preferably 10 mass ppm or less. More preferably 9 mass ppm or less, further preferably 8 mass ppm or less, still more preferably 7 mass ppm or less, still more preferably 6 mass ppm or less, particularly preferably 5 mass ppm or less, 4 mass ppm or less, It may be 3 mass ppm or less, 2 mass ppm or less, 1 mass ppm or less, 0.70 mass ppm or less, 0.50 mass ppm or less, 0.30 mass ppm or less, 0.20 mass ppm or less.
The content of isoamyl acetate can be measured by gas chromatography.
To adjust the content of isoamyl acetate, add diluted water or carbonated water, add fragrance containing isoamyl acetate, amount of fragrance containing isoamyl acetate, amount of raw material containing isoamyl acetate, add raw material containing isoamyl acetate, raw materials containing isoamyl acetate. Type, amount of isoamyl acetate substrate, amount of raw material used as substrate for isoamyl acetate, type of enzyme, amount of enzyme added, timing of enzyme addition, saccharification time in the preparation tank, protein decomposition time in the preparation tank, preparation The pH in the tank, the pH in the preparation process (the process of producing wheat juice from the addition of malt to the addition of yeast), the amount of acid added for pH adjustment, and the timing of pH adjustment (at the time of preparation, fermentation, fermentation completion) Time, before beer filtration, after beer filtration, etc.), set temperature and holding time of each temperature range when preparing wheat juice (including during saccharification), original extract concentration of pre-fermentation liquid, original extract concentration in fermentation process, Fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, fermentation removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.) can be appropriately set. ..
 本発明のビールテイスト飲料の最終発酵度(LA)は60%以下であるが、エンタノール含有量を1.5(v/v)%未満に抑えながらビールテイスト飲料らしい発酵感をより向上させる観点から、最終発酵度(LA)は15%以上が好ましく、16%以上がより好ましく、17%以上がさらに好ましく、18%以上がよりさらに好ましく、19%以上がよりさらに好ましく、20%以上がよりさらに好ましく、21%以上がよりさらに好ましく、23%以上がよりさらに好ましく、25%以上がよりさらに好ましく、27%以上がよりさらに好ましく、29%以上がより好ましく、31%以上がさらに好ましく、また、57%以下が好ましく、55%以下がより好ましく、52%以下がさらに好ましい。また、ビールテイスト飲料の最終発酵度(LA)は34%以上、37%以上、または40%以上であってもよく、48%以下、45%以下、42%以下、41%以下、40%以下、39%以下、38%以下、37%以下、36%以下、35%以下、34%以下、33%以下、32%以下、または、31%以下であってもよい。 The final fermentation degree (LA) of the beer-taste beverage of the present invention is 60% or less, but the viewpoint of further improving the fermentation feeling like a beer-taste beverage while suppressing the entanol content to less than 1.5 (v / v)%. Therefore, the final fermentation degree (LA) is preferably 15% or more, more preferably 16% or more, further preferably 17% or more, further preferably 18% or more, further preferably 19% or more, and more preferably 20% or more. More preferably, 21% or more is even more preferable, 23% or more is even more preferable, 25% or more is even more preferable, 27% or more is even more preferable, 29% or more is more preferable, 31% or more is even more preferable, and more. , 57% or less, more preferably 55% or less, still more preferably 52% or less. The final fermentation degree (LA) of the beer-taste beverage may be 34% or more, 37% or more, or 40% or more, 48% or less, 45% or less, 42% or less, 41% or less, 40% or less. , 39% or less, 38% or less, 37% or less, 36% or less, 35% or less, 34% or less, 33% or less, 32% or less, or 31% or less.
 本明細書において、「最終発酵度(LA)」は、改訂BCOJビール分析法 ビール酒造組合 国際技術委員会 〔分析委員会〕編2013年増補改訂(公益財団法人日本醸造協会)8.11最終発酵度に記載の内容により測定することができる。 In this specification, "final fermentation degree (LA)" is the revised BCOJ beer analysis method, Beer Brewing Association, International Technical Committee [Analysis Committee], 2013 supplementary revision (Japan Brewing Society) 8.11 final fermentation degree. It can be measured according to the contents described.
 ビールテイスト飲料の最終発酵度(LA)の値は、たとえば、原料の糖化条件、原材料の種類や配合量等を調整することにより、制御することができる。たとえば、糖化酵素が高い活性を示す温度にて、糖化時間を長くすれば、でんぷんや高分子の糖の分解が進み、最終発酵度(LA)の値が大きくなる。糖化力の強い麦芽を使用することで最終発酵度(LA)の値が大きくなる。また、酵母が資化しやすい糖液を原料として使用することで最終醗酵度(LA)の値が大きくなる。 The final fermentation degree (LA) value of the beer-taste beverage can be controlled, for example, by adjusting the saccharification conditions of the raw materials, the type and blending amount of the raw materials, and the like. For example, if the saccharification time is lengthened at a temperature at which the saccharifying enzyme exhibits high activity, the decomposition of starch and high molecular weight sugar proceeds, and the final fermentation degree (LA) value increases. By using malt with strong saccharification power, the final fermentation degree (LA) value becomes large. In addition, the final fermentation degree (LA) value is increased by using a sugar solution that is easily assimilated by yeast as a raw material.
 ビールテイスト飲料に不適な酸味を付与しないために、本発明の飲料の乳酸の含有量は
1000質量ppm以下が好ましく、900質量ppm以下がより好ましく、700質量ppm以下がさらに好ましく、500質量ppm以下がよりさらに好ましく、300質量ppm以下がさらに好ましく、200質量ppm以下が特に好ましい。
 また、ビールテイスト飲料のpHを下げて微生物保証を得るために、本発明の飲料の乳酸の含有量は、50質量ppm以上が好ましく、70質量ppm以上がより好ましく、100質量ppm以上がさらに好ましく、110質量ppm以上がよりさらに好ましく、130質量ppm以上がさらに好ましく、150質量ppm以上が特に好ましい。
In order not to impart an unsuitable acidity to the beer-taste beverage, the lactic acid content of the beverage of the present invention is preferably 1000% by mass or less, more preferably 900% by mass or less, further preferably 700% by mass or less, and further preferably 500% by mass or less. Is even more preferable, 300 mass ppm or less is further preferable, and 200 mass ppm or less is particularly preferable.
Further, in order to lower the pH of the beer-taste beverage and obtain a microbial guarantee, the lactic acid content of the beverage of the present invention is preferably 50% by mass or more, more preferably 70% by mass or more, still more preferably 100% by mass or more. , 110 mass ppm or more is further preferable, 130 mass ppm or more is further preferable, and 150 mass ppm or more is particularly preferable.
 同様に、ビールテイスト飲料に不適な酸味を付与しないために、本発明の飲料のリン酸の含有量は1000質量ppm以下が好ましく、900質量ppm以下がより好ましく、700質量ppm以下がさらに好ましく、500質量ppm以下がよりさらに好ましく、300質量ppm以下がさらに好ましく、200質量ppm以下が特に好ましい。
 また、ビールテイスト飲料のpHを下げて微生物保証を得るために、本発明の飲料のリン酸の含有量は50質量ppm以上が好ましく、70質量ppm以上がより好ましく、100質量ppm以上がさらに好ましく、120質量ppm以上がよりさらに好ましく、140質量ppm以上がさらに好ましく、160質量ppm以上が特に好ましい。
Similarly, the phosphate content of the beverage of the present invention is preferably 1000% by mass or less, more preferably 900% by mass or less, still more preferably 700% by mass or less, in order not to impart an unsuitable acidity to the beer-taste beverage. 500 mass ppm or less is further preferable, 300 mass ppm or less is further preferable, and 200 mass ppm or less is particularly preferable.
Further, in order to lower the pH of the beer-taste beverage and obtain a microbial guarantee, the phosphate content of the beverage of the present invention is preferably 50% by mass or more, more preferably 70% by mass or more, still more preferably 100% by mass or more. , 120 mass ppm or more is further preferable, 140 mass ppm or more is further preferable, and 160 mass ppm or more is particularly preferable.
 本発明のビールテイスト飲料は乳酸およびリン酸を含むことが好ましい。
 乳酸とリン酸の含有量の合計は、ビールテイスト飲料に程よい酸味を付与する観点から、1700質量ppm以下が好ましく、1300質量ppm以下がより好ましく、1000質量ppm以下がさらに好ましく、700質量ppm以下がよりさらに好ましく、500質量ppm以下が特に好ましく、また、飲料のpHを下げて微生物保証を得る観点から、100質量ppm以上が好ましく、150質量ppm以上がさらに好ましく、200質量ppm以上がよりさらに好ましく、300質量ppm以上が特に好ましい。
The beer-taste beverage of the present invention preferably contains lactic acid and phosphoric acid.
The total content of lactic acid and phosphoric acid is preferably 1700 mass ppm or less, more preferably 1300 mass ppm or less, further preferably 1000 mass ppm or less, and further preferably 700 mass ppm or less, from the viewpoint of imparting a moderate acidity to the beer-taste beverage. Is even more preferable, 500 mass ppm or less is particularly preferable, and from the viewpoint of lowering the pH of the beverage to obtain a microbial guarantee, 100 mass ppm or more is more preferable, 150 mass ppm or more is further preferable, and 200 mass ppm or more is further more preferable. It is preferable, and 300 mass ppm or more is particularly preferable.
 本発明のビールテイスト飲料の乳酸およびリン酸の含有量は、たとえば、乳酸、リン酸、乳酸またはリン酸の含有量の多い原材料の使用量を調整することによって制御できる。
 なお、本発明のビールテイスト飲料は乳酸およびリン酸以外の有機酸を含んでもよい。
 乳酸およびリン酸以外の有機酸の含有量は、乳酸およびリン酸の合計量100質量部に対して、100質量部未満、70質量部未満、50質量部未満、20質量部未満、10質量部未満、5質量部未満、1質量部未満、0.1質量部未満、または0質量部とすることができる。
The content of lactic acid and phosphoric acid in the beer-taste beverage of the present invention can be controlled, for example, by adjusting the amount of raw materials having a high content of lactic acid, phosphoric acid, lactic acid or phosphoric acid.
The beer-taste beverage of the present invention may contain an organic acid other than lactic acid and phosphoric acid.
The content of organic acids other than lactic acid and phosphoric acid is less than 100 parts by mass, less than 70 parts by mass, less than 50 parts by mass, less than 20 parts by mass, and 10 parts by mass with respect to 100 parts by mass of the total amount of lactic acid and phosphoric acid. Less than, less than 5 parts by mass, less than 1 part by mass, less than 0.1 parts by mass, or 0 parts by mass.
 本発明のビールテイスト飲料の乳酸およびリン酸の含有量は、たとえば、高速液体クロマトグラフィーによって測定することができる。 The content of lactic acid and phosphoric acid in the beer-taste beverage of the present invention can be measured by, for example, high performance liquid chromatography.
 本発明の一態様のビールテイスト飲料において、リアルエキス濃度は、好ましくは1(w/w)%以上、より好ましくは2(w/w)%以上、さらに好ましくは3(w/w)%以上、よりさらに好ましくは4(w/w)%以上、さらに好ましくは5(w/w)%以上、よりさらに好ましくは6(w/w)%以上、よりさらに好ましくは6.5(w/w)%以上、よりさらに好ましくは7(w/w)%以上、特に好ましくは7.5(w/w)%以上であり、7.7(w/w)%以上、7.8(w/w)%以上、7.9(w/w)%以上、8.0(w/w)%以上であってもよい。また、本発明の一態様のビールテイスト飲料において、リアルエキス濃度は、好ましくは10(w/w)%以下、より好ましくは9.5(w/w)%以下、さらに好ましくは9(w/w)%以下、よりさらに好ましくは8.5(w/w)%以下であり、よりさらに好ましくは8.4(w/w)%以下であり、よりさらに好ましくは8.3(w/w)%以下であり、よりさらに好ましくは8.2(w/w)%以下である。
 リアルエキス濃度は、改訂BCOJビール分析法 ビール酒造組合 国際技術委員会 〔分析委員会〕編2013年増補改訂(公益財団法人日本醸造協会)8.4.3アルコライザー法に記載の方法によって測定することができる。
 リアルエキス濃度の調整は、希釈水または炭酸水の添加、原材料(麦芽、コーングリッツ、糖液等)の種類、麦芽の粉砕粒度、麦芽の粉砕様式(湿式粉砕、乾式粉砕など)、麦芽粉砕時の湿度(調湿の程度)、麦芽粉砕時の温度、麦芽粉砕の際に使用するミルの種類、原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、酵素分解の時間、仕込槽で糖化時間、仕込槽でのタンパク分解時間、仕込槽でのpH、仕込工程(麦芽投入から酵母添加前での麦汁製造工程)でのpH、pH調整の際に使用する酸の添加量、pH調整のタイミング(仕込時、発酵時、発酵完了時、ビール濾過前、ビール濾過後など)、麦汁を調製する際(糖化時含む)の各温度領域の設定温度及び保持時間、麦汁濾過の時間、麦汁濾過時の温度、麦汁濾過時のpH、麦汁濾過での麦汁回収量、麦汁濾過時のスパージング水の量、麦汁濾過時のスパージング水のpH、麦汁濾過時のスパージング水の温度、発酵条件(酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度等)、スピリッツや醸造アルコールなどの添加等を適宜設定して行うことができる。
In the beer-taste beverage of one aspect of the present invention, the real extract concentration is preferably 1 (w / w)% or more, more preferably 2 (w / w)% or more, still more preferably 3 (w / w)% or more. , More preferably 4 (w / w)% or more, still more preferably 5 (w / w)% or more, even more preferably 6 (w / w)% or more, still more preferably 6.5 (w / w). )% Or more, more preferably 7 (w / w)% or more, particularly preferably 7.5 (w / w)% or more, 7.7 (w / w)% or more, 7.8 (w / w /). It may be w)% or more, 7.9 (w / w)% or more, and 8.0 (w / w)% or more. Further, in the beer-taste beverage of one aspect of the present invention, the real extract concentration is preferably 10 (w / w)% or less, more preferably 9.5 (w / w)% or less, still more preferably 9 (w / w / w / w / It is w)% or less, more preferably 8.5 (w / w)% or less, still more preferably 8.4 (w / w)% or less, and even more preferably 8.3 (w / w). )% Or less, and even more preferably 8.2 (w / w)% or less.
The real extract concentration can be measured by the method described in the revised BCOJ Beer Analysis Method Beer Brewing Association International Technical Committee [Analysis Committee] ed. can.
The real extract concentration can be adjusted by adding diluted water or carbonated water, the type of raw material (wort, corn writ, sugar solution, etc.), wort crushed grain size, wort crushing method (wet crushing, dry crushing, etc.), and wort crushing. Humidity (degree of humidity control), temperature at wort crushing, type of mill used for wort crushing, amount of raw materials, type of enzyme, amount of enzyme added, timing of addition of enzyme, time of enzymatic decomposition, preparation Saccharification time in the tank, protein decomposition time in the preparation tank, pH in the preparation tank, pH in the preparation process (wort production process from wort injection to before wort addition), amount of acid added for pH adjustment , Timing of pH adjustment (at the time of preparation, at the time of fermentation, at the time of completion of fermentation, before beer filtration, after beer filtration, etc.), when preparing wort (including during saccharification), set temperature and holding time of each temperature range, wort Filtering time, temperature during wort filtration, pH during wort filtration, wort recovery amount during wort filtration, amount of sparging water during wort filtration, pH of sparging water during wort filtration, wort Sparging water temperature during filtration, fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast addition amount, yeast growth number, yeast removal timing, fermentation temperature, fermentation time, pressure setting, carbon dioxide concentration, etc.), spirits And the addition of wort or the like can be appropriately set.
 一般的に、エタノール含有量が一般的なビールテイスト飲料よりも低い飲料は、発酵によって得られる有機酸の含有量が低くなりやすいため、ビールらしい後味のすっきり感が不足しやすいが、本発明の一態様のビールテイスト飲料はpHを2.6~4.1とすることによって、ビールらしい後味のすっきり感を付与することができる。
 ビールテイスト飲料のpHの上限は、好ましくは4.0未満、より好ましくは3.9以下、さらに好ましくは3.8以下、よりさらに好ましくは3.7以下、特に好ましくは3.6以下である。また、ビールテイスト飲料のpHの下限は、好ましくは2.7以上、より好ましくは2.8以上、さらに好ましくは3.0以上であり、よりさらに好ましくは3.1以上、よりさらに好ましくは3.2以上であり、特に好ましくは3.3以上である。
 pHの調整は、希釈水または炭酸水の添加、原材料(麦芽、コーングリッツ、糖液等)の種類、原材料の量、酵素の種類、酵素の添加量、酵素の添加のタイミング、仕込槽で糖化時間、仕込槽でのタンパク分解時間、仕込槽でのpH、仕込工程(麦芽投入から酵母添加前での麦汁製造工程)でのpH、pH調整の際に使用する酸の種類(乳酸、リン酸、リンゴ酸、酒石酸、クエン酸など)、pH調整の際に使用する酸の添加量、pH調整のタイミング(仕込時、発酵時、発酵完了時、ビール濾過前、ビール濾過後など)、麦汁を調製する際(糖化時含む)の各温度領域の設定温度及び保持時間、発酵前液のオリジナルエキス濃度、発酵工程でのオリジナルエキス濃度、発酵条件(酸素濃度、通気条件、酵母品種、酵母の添加量、酵母増殖数、酵母の除去タイミング、発酵温度、発酵時間、圧力設定、二酸化炭素濃度等)等を適宜設定して行うことができる。
In general, beverages having a lower ethanol content than general beer-taste beverages tend to have a low content of organic acids obtained by fermentation, so that the refreshing aftertaste of beer tends to be insufficient. By setting the pH of the beer-taste beverage of one aspect to 2.6 to 4.1, a refreshing aftertaste like beer can be imparted.
The upper limit of the pH of the beer-taste beverage is preferably less than 4.0, more preferably 3.9 or less, still more preferably 3.8 or less, still more preferably 3.7 or less, and particularly preferably 3.6 or less. .. The lower limit of the pH of the beer-taste beverage is preferably 2.7 or more, more preferably 2.8 or more, still more preferably 3.0 or more, still more preferably 3.1 or more, still more preferably 3. It is .2 or more, and particularly preferably 3.3 or more.
To adjust the pH, add diluted water or carbonated water, the type of raw material (fermentation, corn glitz, sugar solution, etc.), the amount of raw material, the type of enzyme, the amount of enzyme added, the timing of enzyme addition, and the saccharification time in the preparation tank. , Protein decomposition time in the preparation tank, pH in the preparation tank, pH in the preparation process (beer production process from malt injection to before yeast addition), type of acid used for pH adjustment (lactic acid, phosphoric acid) , Apple acid, tartrate acid, citric acid, etc.), amount of acid used for pH adjustment, timing of pH adjustment (at the time of preparation, fermentation, completion of fermentation, before beer filtration, after beer filtration, etc.), wheat juice Set temperature and holding time of each temperature range when preparing (including during saccharification), original extract concentration of pre-fermentation liquid, original extract concentration in fermentation process, fermentation conditions (oxygen concentration, aeration conditions, yeast varieties, yeast) The addition amount, the number of yeast growths, the timing of removing yeast, the fermentation temperature, the fermentation time, the pressure setting, the carbon dioxide concentration, etc.) can be appropriately set.
 本発明の一態様のビールテイスト飲料の色は、特に限定されないが、通常のビールのような琥珀色や黄金色、黒ビールのような黒色、または、無色透明であってもよく、あるいは着色料などを添加して、所望の色を付けてもよい。飲料の色は、肉眼でも判別することができるが、全光線透過率や色度等によって規定してもよい。 The color of the beer-taste beverage of one aspect of the present invention is not particularly limited, but may be amber or golden like ordinary beer, black like black beer, or colorless and transparent, or a coloring agent. Etc. may be added to give a desired color. The color of the beverage can be discriminated by the naked eye, but it may be defined by the total light transmittance, the chromaticity, or the like.
 本発明の一態様のビールテイスト飲料は、飲料が容器に詰められた態様であればよく、容器の例としては、たとえば、ビン、ペットボトル、缶、または樽が挙げられるが、特に持ち運びが容易であるとの観点から、缶、ビンまたはペットボトルが好ましい。 The beer-taste beverage of one aspect of the present invention may be any form in which the beverage is packed in a container, and examples of the container include, for example, bottles, PET bottles, cans, or barrels, but it is particularly easy to carry. From the viewpoint of the above, cans, bottles or PET bottles are preferable.
1.1 原材料
 本発明の一態様のビールテイスト飲料の主な原材料として、水と共に麦芽を用いてもよく、また、麦芽を用いなくてもよい。さらにホップを用いてもよく、その他に、蒸留酒、甘味料、水溶性食物繊維、苦味料または苦味付与剤、酸化防止剤、香料、酸味付与物質等を用いてもよい。
1.1 Raw materials Malt may or may not be used with water as the main raw material for the beer-taste beverage of one aspect of the present invention. Further, hops may be used, and in addition, distilled liquor, sweetener, water-soluble dietary fiber, bitterness or bitterness-imparting agent, antioxidant, flavor, acidity-imparting substance and the like may be used.
 原材料として麦芽を用いる場合、当該麦芽とは、大麦、小麦、ライ麦、カラス麦、オート麦、ハト麦、エン麦などの麦類の種子を発芽させて乾燥させ、除根したものをいい、産地や品種は、いずれのものであってもよい。
 本発明の一態様においては、用いる麦芽としては、大麦麦芽が好ましい。大麦麦芽は、日本のビールテイスト飲料の原料として最も一般的に用いられる麦芽の1つである。大麦には、二条大麦、六条大麦などの種類があるが、いずれを用いてもよい。さらに、通常麦芽のほか、色麦芽なども用いることができる。なお、色麦芽を用いる際には、種類の異なる色麦芽を適宜組み合わせて用いてもよいし、一種類の色麦芽を用いてもよい。
When malt is used as a raw material, the malt refers to malt seeds such as barley, wheat, rye, oats, oats, adlay, and oats that have been germinated, dried, and rooted. The variety may be any.
In one aspect of the present invention, barley malt is preferable as the malt to be used. Barley malt is one of the most commonly used malts as a raw material for Japanese beer-taste beverages. There are various types of barley such as two-row barley and six-row barley, but any of them may be used. Further, in addition to normal malt, colored malt and the like can also be used. When using colored malt, different types of colored malt may be used in appropriate combinations, or one type of colored malt may be used.
 また、麦芽と共に、麦芽以外の穀物を用いてもよい。そのような穀物としては、たとえば、麦芽には該当しない麦(大麦、小麦、ライ麦、カラス麦、オート麦、ハト麦、エン麦等)、米(白米、玄米等)、とうもろこし、こうりゃん、ばれいしょ、豆(大豆、えんどう豆等)、そば、ソルガム、粟、ひえ、およびそれらから得られたデンプン、これらの抽出物(エキス)等が挙げられる。 Also, grains other than malt may be used together with malt. Such grains include, for example, wheat that does not fall under malt (barley, wheat, rye, crow barley, oats, pigeons, starch, etc.), rice (white rice, brown rice, etc.), corn, corn, potatoes, etc. , Beans (soybeans, oats, etc.), buckwheat, sorghum, oats, hie, starch obtained from them, extracts (extracts) thereof and the like.
 ビールテイスト飲料の原材料に麦芽を用いない場合には、炭素源を含有する液糖、麦芽以外の上述の穀物等のアミノ酸含有材料としての窒素源を用いことが好ましい。 When malt is not used as a raw material for beer-taste beverages, it is preferable to use a liquid sugar containing a carbon source and a nitrogen source as an amino acid-containing material such as the above-mentioned grains other than malt.
 本発明の一態様で用いるホップの形態としては、たとえば、ペレットホップ、粉末ホップ、ホップエキス等が挙げられる。また、用いるホップは、イソ化ホップ、還元ホップ等のホップ加工品を用いてもよい。
 ホップの添加量としては、適宜調製されるが、飲料全量に対して、好ましくは0.0001~1質量%である。
Examples of the hop form used in one aspect of the present invention include pellet hops, powdered hops, hop extracts and the like. Further, as the hop to be used, a processed hop product such as an isodized hop or a reduced hop may be used.
The amount of hops added is appropriately adjusted, but is preferably 0.0001 to 1% by mass with respect to the total amount of the beverage.
 本発明の一態様のビールテイスト飲料は、アルコール成分として、予め調製された蒸留酒を含むことができる。このような蒸留酒としては、特に制限されるものではないが、たとえば、原料アルコール、スピリッツ、ウォッカ、ラム、テキーラ、ジン、焼酎などが挙げられる。
 ここで、スピリッツとは、麦、米、そば、とうもろこし等の穀物を原料として、麦芽又は必要により酵素剤を用いて糖化し、酵母を用いて発酵させた後、さらに蒸留して得られる酒類を意味する。
The beer-taste beverage of one aspect of the present invention can contain a pre-prepared distilled liquor as an alcohol component. Such distilled liquor is not particularly limited, and examples thereof include raw alcohol, spirits, vodka, rum, tequila, gin, and shochu.
Here, spirits are alcoholic beverages obtained by using grains such as wheat, rice, buckwheat, and corn as raw materials, saccharifying them with malt or, if necessary, using an enzyme agent, fermenting them with yeast, and then further distilling them. means.
 甘味料としては、穀物由来のデンプンを酸または酵素等で分解した市販の糖化液、市販の水飴等の糖類、三糖類以上の糖、糖アルコール、ステビア等の天然甘味料、人工甘味料等が挙げられる。
 これらの糖類の形態は、溶液等の液体であってもよく、粉末等の固体であってもよい。
 また、デンプンの原料穀物の種類、デンプンの精製方法、および酵素や酸による加水分解等の処理条件についても特に制限はない。たとえば、酵素や酸による加水分解の条件を適宜設定することにより、マルトースの比率を高めた糖類を用いてもよい。その他、スクロース、フルクトース、グルコース、マルトース、トレハロース、マルトトリオースおよびこれらの溶液(糖液)等を用いることもできる。
 また、人工甘味料としては、たとえば、アスパルテーム、アセスルファムカリウム(アセスルファムK)、スクラロース等が挙げられる。
Examples of the sweetener include a commercially available saccharified solution obtained by decomposing starch derived from grains with an acid or an enzyme, commercially available sugars such as starch syrup, sugars of trisaccharides or higher, sugar alcohols, natural sweeteners such as stevia, and artificial sweeteners. Can be mentioned.
The form of these saccharides may be a liquid such as a solution or a solid such as a powder.
Further, there are no particular restrictions on the type of starch raw material grain, the method for purifying starch, and the treatment conditions such as hydrolysis with enzymes or acids. For example, a saccharide having an increased proportion of maltose may be used by appropriately setting the conditions for hydrolysis with an enzyme or acid. In addition, sucrose, fructose, glucose, maltose, trehalose, maltotriose, and solutions thereof (sugar solution) can also be used.
Examples of the artificial sweetener include aspartame, acesulfame potassium (acesulfame K), sucralose and the like.
 水溶性食物繊維としては、たとえば、難消化性デキストリン、ポリデキストロース、グアーガム分解物、ペクチン、グルコマンナン、アルギン酸、ラミナリン、フコイジン、カラギーナン等が挙げられ、安定性や安全性等の汎用性の観点から、難消化性デキストリンまたはポリデキストロースが好ましい。 Examples of the water-soluble dietary fiber include indigestible dextrin, polydextrose, guar gum decomposition product, pectin, glucomannan, alginic acid, laminarin, fucoidin, carrageenan, etc. from the viewpoint of versatility such as stability and safety. , Indigestible dextrin or polydextrose is preferred.
 本発明の一態様のビールテイスト飲料において、苦味は、ホップ等によって付与することが好ましいが、ホップと共に、もしくは、ホップに代えて、苦味料または苦味付与剤を用いてもよい。
 苦味料または苦味付与剤としては、特に限定されず、通常のビールや発泡酒に苦味付与剤として用いられるものが使用でき、たとえば、ニガヨモギ、マンネンロウ、レイシ、姫茴香、杜松実、セージ、迷迭香、マンネンタケ、月桂樹、クワシン、ナリンジン、アブシンチン、ニガヨモギ抽出物、柑橘抽出物、ニガキ抽出物、コーヒー抽出物、茶抽出物、ゴーヤ抽出物、ハス胚芽抽出物、キダチアロエ抽出物、マンネンロウ抽出物、レイシ抽出物、ローレル抽出物、セージ抽出物、キャラウェイ抽出物等が挙げられる。
In the beer-taste beverage of one aspect of the present invention, the bitterness is preferably imparted by hops or the like, but a bitterness agent or a bitterness-imparting agent may be used together with or in place of hops.
The bitterness agent or bitterness-imparting agent is not particularly limited, and those used as a bitterness-imparting agent in ordinary beer and effervescent liquor can be used. Sage, Mannentake, Laurel, Kuwashin, Naringin, Absinthin, Wormwood extract, Citrus extract, Wormwood extract, Coffee extract, Tea extract, Bitter gourd extract, Hass germ extract, Kidachi aloe extract, Mannen wax extract, Examples thereof include wormwood extract, laurel extract, sage extract, and caraway extract.
 酸化防止剤としては、特に限定されず、通常のビールや発泡酒に酸化防止剤として用いられるものが使用でき、たとえば、アスコルビン酸、エリソルビン酸、およびカテキン等が挙げられる。 The antioxidant is not particularly limited, and those used as antioxidants in ordinary beer and low-malt beer can be used, and examples thereof include ascorbic acid, erythorbic acid, and catechin.
 香料としては、特に限定されず、一般的なビール香料を用いることができる。ビール香料は、ビール様の風味付けのために用いるものである。
 ビール香料としては、エステルや高級アルコール等が挙げられ、具体的には、酢酸エチル、酢酸イソアミル、n-プロパノール、イソブタノール、カプロン酸エチル、4ビニルグアイアコールおよびアセトアルデヒド等が挙げられる。
The fragrance is not particularly limited, and a general beer fragrance can be used. Beer flavors are used for beer-like flavoring.
Examples of the beer fragrance include esters, higher alcohols and the like, and specific examples thereof include ethyl acetate, isoamyl acetate, n-propanol, isobutanol, ethyl caproate, 4-vinylguaiacol and acetaldehyde.
 本発明の一態様のビールテイスト飲料は、乳酸およびリン酸以外の酸味付与物質を含有してもよい。他の酸味付与物質を用いることで、飲料の発酵感と後味のすっきり感をさらに向上させることができる。
 乳酸およびリン酸以外の酸味付与物質としては、酸味を付与するものであれば特に限定されないが、たとえば、クエン酸、グルコン酸、リンゴ酸、フィチン酸、酢酸、コハク酸、酒石酸、ピルビン酸、ピログルタミン酸、醸造酢、それらの塩を用いることができる。これらの酸味付与物質の中でも、クエン酸、酒石酸、醸造酢、それらの塩が好ましく、醸造酢がより好ましい。また、酸味付与物質として、2以上の成分を併用してもよい。
 本発明の一態様のビールテイスト飲料において、乳酸およびリン酸を含む全ての酸味付与物質の含有量は、後味のすっきり感を向上させる観点から、酸味付与物質が酸度0.01~0.4%であることが好ましい。「酸度」とは、日本農林規格(平成18年8月8日農水告第1127号)に定められた酸度の測定方法に基づいて算出されるものを意味する。
The beer-taste beverage of one aspect of the present invention may contain an acidity-imparting substance other than lactic acid and phosphoric acid. By using other acidity-imparting substances, the fermented feeling and the refreshing feeling of the aftertaste of the beverage can be further improved.
The acidity-imparting substance other than lactic acid and phosphoric acid is not particularly limited as long as it imparts acidity, but for example, citric acid, gluconic acid, malic acid, phytic acid, acetic acid, succinic acid, tartaric acid, pyruvate, and pyro. Glutamic acid, brewed vinegar and salts thereof can be used. Among these acidity-imparting substances, citric acid, tartaric acid, brewed vinegar, and salts thereof are preferable, and brewed vinegar is more preferable. In addition, two or more components may be used in combination as the acidity-imparting substance.
In the beer-taste beverage of one aspect of the present invention, the content of all acidity-imparting substances including lactic acid and phosphoric acid is such that the acidity-imparting substance has an acidity of 0.01 to 0.4% from the viewpoint of improving the refreshing feeling of the aftertaste. Is preferable. "Acidity" means what is calculated based on the acidity measuring method specified in the Japanese Agricultural Standards (August 8, 2006, Agricultural Water Declaration No. 1127).
1.2 炭酸ガス
 本発明の一態様のビールテイスト飲料に含まれる炭酸ガスは、炭酸水との混和によって加えてもよく、または原料液に炭酸ガスを直接添加してもよい。
1.2 Carbon dioxide gas The carbon dioxide gas contained in the beer-taste beverage of one aspect of the present invention may be added by mixing with carbonated water, or carbon dioxide gas may be added directly to the raw material liquid.
 本発明のビールテイスト飲料の炭酸ガス濃度は、好ましくは0.30(w/w)%以上、より好ましくは0.35(w/w)%以上、更に好ましくは0.40(w/w)%以上で、より更に好ましくは0.42(w/w)%以上、特に好ましくは0.45(w/w)%以上であり、また、好ましくは0.80(w/w)%以下、より好ましくは0.70(w/w)%以下、更に好ましくは0.60(w/w)%以下、より更に好ましくは0.57(w/w)以下、特に好ましくは0.55(w/w)%以下である。
 なお、本明細書において、炭酸ガス濃度は、対象となる飲料が入った容器を時々振りながら20℃の水槽に30分間以上浸して、当該飲料が20℃になるよう調整した後に、ガスボリューム測定装置(例えば、GVA-500(京都電子工業株式会社製)等)を用いて測定することができる。
 ビールテイスト飲料に含まれる炭酸ガスの量は、飲料の炭酸ガス圧によって表されるが、本発明の効果を妨げない限り、特に限定されない。典型的には、飲料の炭酸ガス圧の上限は5.0kg/cm、4.5kg/cm、または4.0kg/cmであり、下限は0.20kg/cm、0.50kg/cm、または1.0kg/cmであり、これらの上限および下限のいずれを組み合わせてもよい。たとえば、飲料の炭酸ガス圧は、0.20kg/cm以上5.0kg/cm以下、0.50kg/cm以上4.5kg/cm以下、または、1.0kg/cm以上4.0kg/cm以下であってよい。
 本明細書において、ガス圧とは、特別な場合を除き、容器内におけるガス圧をいう。
 圧力の測定は、当業者によく知られた方法、たとえば20℃にした試料をガス内圧計に固定した後、一度ガス内圧計の活栓を開いてガスを抜き、再び活栓を閉じ、ガス内圧計を振り動かして指針が一定の位置に達したときの値を読み取る方法を用いて、または市販のガス圧測定装置を用いて測定することができる。
The carbon dioxide concentration of the beer-taste beverage of the present invention is preferably 0.30 (w / w)% or more, more preferably 0.35 (w / w)% or more, still more preferably 0.40 (w / w). % Or more, more preferably 0.42 (w / w)% or more, particularly preferably 0.45 (w / w)% or more, and preferably 0.80 (w / w)% or less. More preferably 0.70 (w / w)% or less, still more preferably 0.60 (w / w)% or less, still more preferably 0.57 (w / w) or less, and particularly preferably 0.55 (w). / W)% or less.
In the present specification, the carbon dioxide gas concentration is measured by immersing the container containing the target beverage in a water tank at 20 ° C. for 30 minutes or more while shaking it occasionally to adjust the temperature of the beverage to 20 ° C. The measurement can be performed using an apparatus (for example, GVA-500 (manufactured by Kyoto Electronics Industry Co., Ltd.)).
The amount of carbon dioxide gas contained in the beer-taste beverage is represented by the carbon dioxide gas pressure of the beverage, but is not particularly limited as long as the effect of the present invention is not impaired. Typically, the upper limit of the carbon dioxide gas pressure of the beverage is 5.0kg / cm 2, 4.5kg / cm 2 or 4.0 kg / cm 2,, the lower limit is 0.20kg / cm 2, 0.50kg / It is cm 2 or 1.0 kg / cm 2 , and any of these upper and lower limits may be combined. For example, carbon dioxide gas pressure of the beverage, 0.20 kg / cm 2 or more 5.0 kg / cm 2 or less, 0.50 kg / cm 2 or more 4.5 kg / cm 2 or less, or, 1.0 kg / cm 2 or more 4. It may be 0 kg / cm 2 or less.
As used herein, the gas pressure means the gas pressure in the container, except for special cases.
The pressure is measured by a method well known to those skilled in the art, for example, after fixing a sample at 20 ° C to a gas internal pressure gauge, the stopcock of the gas internal pressure gauge is opened once to release the gas, the stopcock is closed again, and the gas internal pressure gauge is measured. It can be measured by using a method of swinging and reading the value when the pointer reaches a certain position, or by using a commercially available gas pressure measuring device.
1.3 その他の添加物
 本発明の一態様のビールテイスト飲料は、本発明の効果を妨げない範囲で、必要に応じて、様々な添加物を添加してもよい。
 そのような添加物としては、たとえば、着色料、泡形成剤、発酵促進剤、酵母エキス、ペプチド含有物等のタンパク質系物質、アミノ酸等の調味料が挙げられる。
 着色料は、飲料にビール様の色を与えるために使用するものであり、カラメル色素などを用いることができる。泡形成剤は、飲料にビール様の泡を形成させるため、あるいは飲料の泡を保持させるために使用するものであり、大豆サポニン、キラヤサポニン等の植物抽出サポニン系物質、コーン、大豆などの植物タンパク、およびペプチド含有物、ウシ血清アルブミン等のタンパク質系物質、酵母エキスなどを適宜使用することができる。
 発酵促進剤は、酵母による発酵を促進させるために使用するものであり、たとえば、酵母エキス、米や麦などの糠成分、ビタミン、ミネラル剤などを単独または組み合わせて使用することができる。
 また、添加物として、安息香酸、安息香酸ナトリウムなどの安息香酸塩、二炭酸ジメチルを添加してもよい。
1.3 Other Additives In the beer-taste beverage of one aspect of the present invention, various additives may be added, if necessary, as long as the effects of the present invention are not impaired.
Examples of such additives include colorants, foam-forming agents, fermentation accelerators, yeast extracts, protein-based substances such as peptide-containing substances, and seasonings such as amino acids.
The colorant is used to give a beer-like color to a beverage, and a caramel color or the like can be used. Foam-forming agents are used to form beer-like foam in beverages or to retain foam in beverages, and are plant-extracted saponin-based substances such as soybean saponin and kiraya saponin, and plants such as corn and soybean. Proteins, peptide-containing substances, protein-based substances such as bovine serum albumin, yeast extracts and the like can be appropriately used.
The fermentation accelerator is used to promote fermentation by yeast, and for example, yeast extract, bran components such as rice and wheat, vitamins, mineral agents and the like can be used alone or in combination.
Further, as an additive, benzoic acid, benzoate such as sodium benzoate, and dimethyl dicarbonate may be added.
2. ビールテイスト飲料の製造方法
本発明のビールテイスト飲料は発酵工程を含む発酵ビールテイスト飲料である。
以下、本発明の一態様のビールテイスト飲料の製造方法を説明する。
2. 2. Method for Producing Beer-Taste Beverage The beer-taste beverage of the present invention is a fermented beer-taste beverage including a fermentation step.
Hereinafter, a method for producing a beer-taste beverage according to one aspect of the present invention will be described.
 本発明のビールテイスト飲料の製造方法としては、特に制限はなく、たとえば、下記工程(1)~(2)を有する方法が挙げられる。
・工程(1):水および麦芽等の各種原材料を含む混合物を糖化処理して発酵原料液を調製する工程。
・工程(2):前記発酵原料液に酵母を添加して、アルコール発酵を行う工程。
 また、本発明のビールテイスト飲料の製造方法において、必要に応じて、さらに下記工程(3)~(7)の1以上を有してもよい。
・工程(3):ジアセチル含有量を調整する工程。
・工程(4):酢酸エチル含有量を調整する工程。
・工程(5):HS含有量を調整する工程。
・工程(6):エタノール含有量を調整する工程。
・工程(7):乳酸含有量および/またはリン酸含有量を調整する工程。
 以下、上記の各工程について説明する。
The method for producing a beer-taste beverage of the present invention is not particularly limited, and examples thereof include methods having the following steps (1) and (2).
-Step (1): A step of preparing a fermentation raw material liquid by saccharifying a mixture containing various raw materials such as water and malt.
-Step (2): A step of adding yeast to the fermentation raw material liquid to perform alcoholic fermentation.
Further, in the method for producing a beer-taste beverage of the present invention, one or more of the following steps (3) to (7) may be further provided, if necessary.
-Step (3): A step of adjusting the diacetyl content.
-Step (4): A step of adjusting the ethyl acetate content.
· Step (5): H 2 S content step of adjusting the.
-Step (6): A step of adjusting the ethanol content.
-Step (7): A step of adjusting the lactic acid content and / or the phosphoric acid content.
Hereinafter, each of the above steps will be described.
<工程(1)>
 工程(1)は、水および麦芽等の各種原材料を含む混合物を糖化処理して発酵原料液を調製する工程である。
 原材料には穀物を用いることが好ましく、麦、麦芽を用いることがより好ましい。麦芽比率は特に限定されないが、上限値は100%以下、好ましくは90%以下、より好ましくは80%以下、さらに好ましくは70%以下、よりさらに好ましくは60%以下であり、また、下限値は好ましくは10%以上、より好ましくは15%以上、さらに好ましくは17.5%以上、よりさらに好ましくは20%以上、特に好ましくは22.5%以上となるように調整されることが好ましい。
 発酵原料液の調製方法としては、水および麦芽等の原材料を仕込釜または仕込槽に投入し、必要に応じてアミラーゼ等の酵素を添加して混合物を調製した後、糖化処理して得る方法が挙げられる。その後、得られた発酵原料液は、ろ過して煮沸し、清澄タンクにて凝固タンパク等の固形分を取り除くことが好ましい。
<Process (1)>
Step (1) is a step of preparing a fermentation raw material liquid by saccharifying a mixture containing various raw materials such as water and malt.
It is preferable to use grains as raw materials, and it is more preferable to use wheat and malt. The malt ratio is not particularly limited, but the upper limit is 100% or less, preferably 90% or less, more preferably 80% or less, further preferably 70% or less, still more preferably 60% or less, and the lower limit value. It is preferably adjusted to be preferably 10% or more, more preferably 15% or more, still more preferably 17.5% or more, still more preferably 20% or more, and particularly preferably 22.5% or more.
As a method for preparing the fermentation raw material liquid, a method is obtained in which raw materials such as water and malt are put into a charging kettle or a charging tank, and if necessary, an enzyme such as amylase is added to prepare a mixture, and then saccharification treatment is performed. Can be mentioned. After that, it is preferable that the obtained fermentation raw material liquid is filtered and boiled to remove solids such as coagulated protein in a clarification tank.
 また、糖化処理に供する混合物として、水および麦芽以外に、ホップ、食物繊維、甘味料、酸化防止剤、苦味付与剤、香料、酸味付与物質、色素等を加えてもよい。
 これらは、糖化処理を行う前に加えてもよく、糖化処理の途中で加えてもよく、糖化処理の終了後に加えてもよい。また、これらは、次工程のアルコール発酵後に加えてもよい。
In addition to water and malt, hops, dietary fiber, sweeteners, antioxidants, bitterness-imparting agents, flavors, acidity-imparting substances, pigments and the like may be added as the mixture to be subjected to the saccharification treatment.
These may be added before the saccharification treatment, may be added during the saccharification treatment, or may be added after the completion of the saccharification treatment. In addition, these may be added after the alcoholic fermentation of the next step.
 本工程において、原材料を加えた前記混合物は、加温し、原材料の澱粉質を糖化させる。
 糖化処理の温度および時間は、使用する麦芽の種類や、麦芽比率、水および麦芽以外の原材料、使用する酵素の種類や量等によって適宜調整する。
In this step, the mixture to which the raw material is added is heated to saccharify the starch of the raw material.
The temperature and time of the saccharification treatment are appropriately adjusted depending on the type of malt used, the malt ratio, raw materials other than water and malt, the type and amount of enzyme used, and the like.
<工程(2)>
 工程(2)は、工程(1)で糖化処理して得られた発酵原料液に、酵母を添加し、アルコール発酵を行う工程である。
 本工程で用いる酵母は、製造すべき発酵飲料の種類、目的とする香味や発酵条件等を考慮して適宜選択することができ、上面発酵酵母を用いてもよく、下面発酵酵母を用いてもよいが、下面発酵酵母を用いることが好ましい。
<Process (2)>
The step (2) is a step of adding yeast to the fermentation raw material liquid obtained by the saccharification treatment in the step (1) to perform alcoholic fermentation.
The yeast used in this step can be appropriately selected in consideration of the type of fermented beverage to be produced, the desired flavor, fermentation conditions, etc., and may be top-fermented yeast or bottom-fermented yeast. Although good, it is preferable to use bottom fermenting yeast.
 酵母は、酵母懸濁液のまま発酵原料液に添加してもよいし、遠心分離あるいは沈降により酵母を濃縮したスラリーを発酵原料液に添加してもよい。また、遠心分離の後、完全に上澄みを取り除いたものを添加してもよい。酵母の発酵原料液への添加量は適宜設定できるが、40×10cells/ml以上であることが好ましく、40×10cells/ml~1×10cells/mlであることがより好ましい。 Yeast may be added to the fermentation raw material solution as it is in a yeast suspension, or a slurry obtained by concentrating yeast by centrifugation or sedimentation may be added to the fermentation raw material solution. Further, after centrifugation, the supernatant may be completely removed before addition. The amount of yeast added to the fermentation feedstock solution can be appropriately set, but is preferably 40 × 10 6 cells / ml or more, and more preferably 40 × 10 6 cells / ml to 1 × 10 8 cells / ml. ..
 アルコール発酵を行う際の発酵条件は、適宜設定することができるが、最終的に得られるビールテイスト飲料中のエタノール含有量が0.4(v/v)%以上1.5(v/v)%未満、ジアセチル含有量が0.07質量ppm未満、および、HSの含有量が5質量ppb未満となるように設定することが好ましい。
 発酵速度が速いと短時間でエタノール含有量が増加するので、通常よりも低い温度で発酵させることが好ましい。発酵温度は好ましくは21℃以下、より好ましくは15℃以下、さらに好ましくは9℃以下、よりさらに好ましくは5℃以下、よりさらに好ましくは4℃以下、よりさらに好ましくは3℃以下、よりさらに好ましくは2℃以下、よりさらに好ましくは1.75℃以下、特に好ましくは1.5℃以下であり、また、好ましくは1℃以上である。
 また、発酵時間が一定時間を超えると、エタノール含有量が増加し、HSおよびジアセチルの産生が増加するので、通常よりも短い時間で発酵させることが好ましい。発酵時間の下限値は好ましくは1時間以上、より好ましくは5時間以上、さらに好ましくは15時間以上、よりさらに好ましくは20時間以上、よりさらに好ましくは25時間以上、よりさらに好ましくは30時間以上、特に好ましくは35時間以上である。また、発酵時間の上限値は好ましくは720時間以下、より好ましくは480時間以下、さらに好ましくは360時間以下、よりさらに好ましくは240時間以下、特に好ましくは200時間以下である。
Fermentation conditions for alcoholic fermentation can be set as appropriate, but the ethanol content in the finally obtained beer-taste beverage is 0.4 (v / v)% or more and 1.5 (v / v). % less, diacetyl content of less than 0.07 mass ppm, and it is preferable to set such that the content of H 2 S is less than 5 mass ppb.
Since the ethanol content increases in a short time when the fermentation rate is high, it is preferable to ferment at a temperature lower than usual. The fermentation temperature is preferably 21 ° C. or lower, more preferably 15 ° C. or lower, still more preferably 9 ° C. or lower, still more preferably 5 ° C. or lower, still more preferably 4 ° C. or lower, still more preferably 3 ° C. or lower, still more preferable. Is 2 ° C. or lower, more preferably 1.75 ° C. or lower, particularly preferably 1.5 ° C. or lower, and preferably 1 ° C. or higher.
Also, when the fermentation time exceeds a predetermined time, the ethanol content is increased, since the production of H 2 S and diacetyl is increased, it is preferable to ferment in a shorter time than usual. The lower limit of the fermentation time is preferably 1 hour or more, more preferably 5 hours or more, still more preferably 15 hours or more, still more preferably 20 hours or more, still more preferably 25 hours or more, still more preferably 30 hours or more. Especially preferably, it is 35 hours or more. The upper limit of the fermentation time is preferably 720 hours or less, more preferably 480 hours or less, still more preferably 360 hours or less, still more preferably 240 hours or less, and particularly preferably 200 hours or less.
 工程(2)のアルコール発酵後は、ろ過機等で酵母を取り除く。
 アルコール発酵工程の前後で、必要に応じて水や香料、酸味付与物質、色素等の添加剤を加える工程を含んでもよい。また、水や香料、酸味付与物質、色素等の添加剤を加える工程において、マルトース含有量、オリジナルエキス濃度、リアルエキス濃度等を調整してもよい。
 アルコール発酵工程の後、エタノールを除去することによって、エタノール含有量を減らすことができる。
After the alcoholic fermentation in step (2), yeast is removed with a filter or the like.
Before and after the alcoholic fermentation step, a step of adding additives such as water, a fragrance, an acidity-imparting substance, and a pigment may be included, if necessary. Further, the maltose content, the original extract concentration, the real extract concentration and the like may be adjusted in the step of adding additives such as water, fragrance, acidity-imparting substance and pigment.
By removing the ethanol after the alcoholic fermentation step, the ethanol content can be reduced.
<工程(3)、工程(4)、工程(5)、工程(6)、工程(7)>
 工程(3)、工程(4)、工程(5)、工程(6)および工程(7)は、工程(1)または工程(2)の中で、また、工程(1)終了後の工程(2)の前後で行ってもよい。ただし、最終的に製造される飲料中の含有量を正確に調整する観点から、工程(1)終了後であって、工程(2)開始前に行うことが好ましい。
 なお、工程(3)、工程(4)、工程(5)、工程(6)および工程(7)の順序は、特に制限はなく、どの工程から行ってもよい。
<Step (3), Step (4), Step (5), Step (6), Step (7)>
Step (3), step (4), step (5), step (6) and step (7) are included in step (1) or step (2) and after step (1) is completed. You may go before and after 2). However, from the viewpoint of accurately adjusting the content in the finally produced beverage, it is preferable to carry out the process after the end of the step (1) and before the start of the step (2).
The order of steps (3), step (4), step (5), step (6) and step (7) is not particularly limited and may be performed from any step.
 工程(3)において、調整前の飲料中のジアセチル含有量を測定し、その測定値の基づき、ジアセチルまたはジアセチルを含む原材料を添加、もしくは水または炭酸水等を加えて希釈して、ジアセチル含有量の上述の範囲となるように調整を行うことが好ましい。ジアセチルは発酵工程で産生される。そこで、工程(2)において、発酵を途中で停止させることによって、ジアセチル含有量を調整させることが好ましい。
 なお、調整前の飲料中の原材料に由来するジアセチルの存在によって、すでにジアセチル含有量が所望の範囲内である場合には、本工程によるジアセチルの添加や水を加えて希釈するといった操作は不要である。
In step (3), the diacetyl content in the beverage before preparation is measured, and based on the measured value, a raw material containing diacetyl or diacetyl is added, or water or carbonated water is added to dilute the diacetyl content. It is preferable to make adjustments so as to be within the above-mentioned range. Diacetyl is produced during the fermentation process. Therefore, in the step (2), it is preferable to adjust the diacetyl content by stopping the fermentation in the middle.
If the diacetyl content is already within the desired range due to the presence of diacetyl derived from the raw materials in the beverage before preparation, it is not necessary to add diacetyl or dilute by adding water in this step. be.
 工程(4)において、調整前の飲料中の酢酸エチル含有量を測定し、その測定値の基づき、酢酸エチルまたは酢酸エチルを含む原材料を添加、もしくは水または炭酸水等を加えて希釈して、酢酸エチル含有量の上述の範囲となるように調整を行うことが好ましい。酢酸エチルは発酵工程で産生される。そこで、工程(2)において、発酵を途中で停止させることによって、酢酸エチル含有量を調整させることが好ましい。
 なお、調整前の飲料中の原材料に由来する酢酸エチルの存在によって、すでに酢酸エチル含有量が所望の範囲内である場合には、本工程による酢酸エチルの添加や水を加えて希釈するといった操作は不要である。
In step (4), the ethyl acetate content in the beverage before preparation is measured, and based on the measured value, a raw material containing ethyl acetate or ethyl acetate is added, or water or carbonated water is added to dilute the mixture. It is preferable to make adjustments so that the ethyl acetate content is within the above range. Ethyl acetate is produced in the fermentation process. Therefore, in the step (2), it is preferable to adjust the ethyl acetate content by stopping the fermentation in the middle.
If the ethyl acetate content is already within the desired range due to the presence of ethyl acetate derived from the raw material in the beverage before preparation, the operation of adding ethyl acetate or adding water to dilute the product is performed in this step. Is unnecessary.
 工程(5)において、調整前の飲料中のHS含有量を測定し、その測定値の基づき、飲料に水または炭酸水等を加えて希釈して、HS含有量の上述の範囲となるように調整を行うことが好ましい。HSは発酵工程で産生される。そこで、工程(2)において、発酵を途中で停止させることによって、HS含有量を調整させることが好ましい。
 なお、調整前の飲料中の原材料に由来するHSの存在によって、すでにHS含有量が所望の範囲内である場合には、本工程による水または炭酸水を加えて希釈するといった操作は不要である。
In step (5), the H 2 S content in the beverage before adjustment is measured, and based on the measured value, water or carbonated water is added to the beverage to dilute it, and the above range of the H 2 S content is obtained. It is preferable to make adjustments so as to be. H 2 S is produced in the fermentation process. Therefore, in the step (2), by stopping the fermentation in the middle, it is preferable to adjust the H 2 S content.
If the H 2 S content is already within the desired range due to the presence of H 2 S derived from the raw material in the beverage before preparation, an operation such as adding water or carbonated water according to this step to dilute the beverage. Is unnecessary.
 工程(6)において、エタノール含有量は、工程(2)の発酵条件を制御すること、蒸留酒等のエタノールを含む原材料を添加することによって調整できるが、工程(2)において、発酵を途中で停止させることによって、エタノール含有量を調整させることが好ましい。また、水または炭酸水等を加えて希釈して、エタノール含有量を調整してもよい。 In the step (6), the ethanol content can be adjusted by controlling the fermentation conditions of the step (2) and adding raw materials containing ethanol such as distilled liquor, but in the step (2), the fermentation is carried out in the middle. It is preferable to adjust the ethanol content by stopping. Further, the ethanol content may be adjusted by adding water, carbonated water or the like to dilute the mixture.
 工程(7)において、調整前の飲料中の乳酸およびリン酸の含有量を測定し、その測定値の基づき、乳酸、リン酸、もしくは2つの酸を添加、または水を加えて希釈して、乳酸含有量およびリン酸含有量が所望の範囲となるように調整を行うことが好ましい。なお、調整前の飲料の乳酸含有量およびリン酸含有量が所望の範囲内である場合には、本工程による調整は不要である。 In step (7), the content of lactic acid and phosphoric acid in the beverage before preparation is measured, and based on the measured value, lactic acid, phosphoric acid, or two acids are added, or water is added to dilute the mixture. It is preferable to adjust the lactic acid content and the phosphoric acid content so as to be in the desired ranges. If the lactic acid content and the phosphoric acid content of the beverage before adjustment are within the desired ranges, adjustment by this step is not necessary.
 このようにして得られたビールテイスト飲料は、所定の容器に充填され、製品として市場に流通する。
 ビールテイスト飲料の容器詰め方法としては、特に限定されず、当業者に周知の容器詰め方法を用いることができる。容器詰め工程によって、ビールテイスト飲料は容器に充填・密閉される。容器詰め工程には、いずれの形態・材質の容器を用いてもよく、容器の例としては、上述のとおりである。
The beer-taste beverage thus obtained is filled in a predetermined container and distributed as a product on the market.
The method for bottling beer-taste beverages is not particularly limited, and a bottling method well known to those skilled in the art can be used. By the containerging process, the beer-taste beverage is filled and sealed in the container. A container of any form and material may be used in the container packing step, and examples of the container are as described above.
 これらの工程後、貯酒工程およびろ過工程等の当業者に周知のビールテイスト飲料の製造で行われる工程を行ってもよい。 After these steps, steps performed in the production of beer-taste beverages well known to those skilled in the art, such as a liquor storage step and a filtration step, may be performed.
 以下、実施例により本発明をさらに詳しく説明するが、本発明はこれらの実施例によっては制限されない。 Hereinafter, the present invention will be described in more detail by way of examples, but the present invention is not limited by these examples.
<飲料の調製>
 粉砕した大麦麦芽を、52℃で保持された温水100Lが入った仕込槽に投入した後、表1および表2に記載された最終発酵度(LA)になるように、糖化液を得るための温度と時間を調整し。具体的には、45~55℃の温度領域で5分~120分、60℃~85℃の温度領域で5分~120分保持しながら、段階的に昇温して、糖化液を調製した。たとえば、実施例1では、52℃で40分保持し、続いて80℃で40分間保持して段階的に昇温した。
 その後、飲料の乳酸およびリン酸の含有量が表1および表2に記載された量になるように添加した。その後、濾過して麦芽粕を除去し、麦汁を得た。得られた麦汁に、表1および表2に記載されたリアルエキス濃度になるように液糖や水を添加し、さらにホップを添加して煮沸を行った。煮沸後の麦汁を固液分離処理し、得られた清澄な麦汁を冷却し、酵母を添加して、表1および表2に記載されたエタノール含有量、HS含有量、ジアセチル含有量、および酢酸エチル含有量になるように、発酵温度、発酵時間、多糖分解酵素の添加量や添加のタイミングを調整して発酵液を調製し、当該発酵液を濾過して、ビールである試験用飲料をそれぞれ調製した。たとえば、実施例1では、発酵温度1.0℃、酵母添加数(60×10cells/mL)で36時間醗酵をおこない、HSが5質量ppb未満、ジアセチルが0.07質量ppm未満、酢酸エチルが0.07質量ppm以上であることを確認し、濾過を実施した。
 なお、各実施例および比較例においては、酵素の種類、添加量および添加のタイミング、糖化液を調製する際の各温度領域の設定温度、保持時間、発酵温度、発酵時間等の発酵条件等を適宜設定し、表1および表2に記載されたエタノール含有量、HS含有量、ジアセチル含有量、酢酸エチル含有量、最終発酵度(LA)、リアルエキス濃度等となるようにそれぞれ調整した。
<Beverage preparation>
After the crushed barley malt is put into a charging tank containing 100 L of warm water maintained at 52 ° C., a saccharified solution is obtained so as to have the final fermentation degree (LA) shown in Tables 1 and 2. Adjust the temperature and time. Specifically, the saccharified solution was prepared by gradually raising the temperature while holding the temperature in the temperature range of 45 to 55 ° C. for 5 to 120 minutes and in the temperature range of 60 ° C. to 85 ° C. for 5 to 120 minutes. .. For example, in Example 1, the temperature was maintained at 52 ° C. for 40 minutes, and then at 80 ° C. for 40 minutes to gradually raise the temperature.
Then, the content of lactic acid and phosphoric acid in the beverage was added so as to be the amounts shown in Tables 1 and 2. Then, the malt meal was removed by filtration to obtain wort. Liquid sugar and water were added to the obtained wort so as to have the real extract concentrations shown in Tables 1 and 2, and hops were further added and boiled. Wort to solid-liquid separation treatment after boiling, the resulting clear wort is cooled, by the addition of yeast, ethanol content described in Table 1 and Table 2, H 2 S content, diacetyl content A fermentation solution is prepared by adjusting the fermentation temperature, fermentation time, addition amount and timing of addition of polysaccharide-degrading enzyme so as to have the amount and ethyl acetate content, and the fermentation solution is filtered to obtain a beer test. Beverages were prepared respectively. For example, in Example 1, the fermentation temperature 1.0 ° C., subjected to 36 hours fermentation in the number of added yeast (60 × 10 6 cells / mL ), H 2 S is less than 5 mass ppb, diacetyl is less than 0.07 ppm by weight , It was confirmed that ethyl acetate was 0.07 mass ppm or more, and filtration was carried out.
In each Example and Comparative Example, the type of enzyme, the amount and timing of addition, the set temperature of each temperature range when preparing the saccharified solution, the holding time, the fermentation temperature, the fermentation conditions such as the fermentation time, etc. appropriately set, ethanol content described in Table 1 and Table 2, H 2 S content, diacetyl content, ethyl acetate content, the final degree of fermentation (LA), were respectively adjusted such that the real extract concentration, etc. ..
<官能評価> 
 得られた飲料について、日頃から訓練を受けた6人のパネラーが、約4℃に冷却した各飲料について官能評価を行った。実施例1~6および比較例1および2では、乳酸およびリン酸の含有量と「ビールテイスト飲料に不適な酸味」との関係性について検討した。また、実施例7~12および比較例3~7では、ジアセチル、HS等の含有量と「ビールテイスト飲料らしい発酵感」および「ビールテイスト飲料らしくない甘み」との関係性について検討した。
 「ビールテイスト飲料に不適な酸味」、「ビールテイスト飲料らしい発酵感」および「ビールテイスト飲料らしくない甘み」について、下記のスコア基準に基づき、3(最大値)~1(最小値)の範囲で、0.1刻みのスコアにて評価し、6人のパネラーのスコアの平均値を算出した。結果を表1および表2に示す。
 評価に際しては、「ビールテイスト飲料に不適な酸味」が下記基準「1」「2」および「3」に該当するサンプル、「ビールテイスト飲料らしい発酵感」が下記基準「1」「2」および「3」に該当するサンプル、および、「ビールテイスト飲料らしくない甘み」が下記基準「1」「2」および「3」に該当するそれぞれのサンプルを予め用意し、各パネラー間での基準の統一を図った。また、表1および表2のいずれの官能評価においても、同じ飲料に対して、各パネラー間での2.0以上のスコアの値の差異は確認されなかった。
<Sensory evaluation>
The obtained beverages were subjected to sensory evaluation by six trained panelists on each beverage cooled to about 4 ° C. In Examples 1 to 6 and Comparative Examples 1 and 2, the relationship between the contents of lactic acid and phosphoric acid and "acidity unsuitable for beer-taste beverages" was examined. In addition, in Examples 7 to 12 and Comparative Examples 3 to 7, diacetyl, was examined the relationship between the content such as H 2 S and "seems low-alcohol beer fermentation feeling" and "sweet not like low-alcohol beer".
"Unsuitable acidity for beer-taste beverages", "fermentation feeling like beer-taste beverages" and "sweetness not like beer-taste beverages" are in the range of 3 (maximum value) to 1 (minimum value) based on the following score criteria. , The score was evaluated in increments of 0.1, and the average value of the scores of the six panelists was calculated. The results are shown in Tables 1 and 2.
In the evaluation, "unsuitable acidity for beer-taste beverages" is a sample that meets the following criteria "1", "2" and "3", and "fermentation feeling like beer-taste beverage" is the following criteria "1""2" and " Prepare samples that correspond to "3" and samples that correspond to the following criteria "1", "2", and "3" for "sweetness that is not like a beer-taste beverage", and unify the criteria among the panelists. planned. In addition, in both the sensory evaluations in Tables 1 and 2, no difference in score values of 2.0 or more was confirmed between the panelists for the same beverage.
[ビールテイスト飲料に不適な酸味](実施例1~6、比較例1~2)
・「3」:感じない。
・「2」:ほとんど感じない。
・「1」:感じる。
 そして、6人のパネラーの平均値を基に、以下の基準で評価をし、2.0以上を合格とした。
[Sour taste unsuitable for beer-taste beverages] (Examples 1 to 6, Comparative Examples 1 to 2)
・ "3": I don't feel it.
・ "2": I hardly feel it.
・ "1": I feel it.
Then, based on the average value of 6 panelists, the evaluation was made according to the following criteria, and 2.0 or more was passed.
[ビールテイスト飲料らしい醗酵感](実施例7~12、比較例3~7)
・「3」:非常に良い。
・「2」:良い。
・「1」:悪い。
 そして、6人のパネラーの平均値を基に、以下の基準で評価をし、2.0以上を合格とした。
[Fermentation feeling like beer-taste beverage] (Examples 7 to 12, Comparative Examples 3 to 7)
・ "3": Very good.
・ "2": Good.
・ "1": Bad.
Then, based on the average value of 6 panelists, the evaluation was made according to the following criteria, and 2.0 or more was passed.
「ビールテイスト飲料らしくない甘み」(実施例7~12、比較例3~7)
・「3」:感じない。
・「2」:ほとんど感じない。
・「1」:感じる。
 そして、6人のパネラーの平均値を基に、以下の基準で評価をし、2.0以上を合格とした。
"Sweetness not like beer-taste beverage" (Examples 7 to 12, Comparative Examples 3 to 7)
・ "3": I don't feel it.
・ "2": I hardly feel it.
・ "1": I feel it.
Then, based on the average value of 6 panelists, the evaluation was made according to the following criteria, and 2.0 or more was passed.
[ビールテイスト飲料の総合評価](実施例7~12、比較例3~7)
 各パネラーが試飲した際の、「ビールテイスト飲料らしい醗酵感」および「ビールテイスト飲料らしくない甘み」に基づき、下記基準によって3段階で総合評価を行った。
・「A」:「ビールテイスト飲料らしい醗酵感」および「ビールテイスト飲料らしくない甘み」の評価の両者が2.5以上。
・「C」:「ビールテイスト飲料らしい醗酵感」および「ビールテイスト飲料らしくない甘み」の評価のどちらか一方が2未満。
・「B」:「A」および「C」に該当しない。
[Comprehensive evaluation of beer-taste beverages] (Examples 7 to 12, Comparative Examples 3 to 7)
Based on the "fermentation feeling that is typical of beer-taste beverages" and the "sweetness that is not typical of beer-taste beverages" when tasting by each panelist, a comprehensive evaluation was made on a three-point scale according to the following criteria.
-"A": Both the evaluations of "fermentation feeling like beer-taste beverage" and "sweetness not like beer-taste beverage" are 2.5 or more.
-"C": Either one of the evaluations of "fermentation feeling like beer-taste beverage" and "sweetness not like beer-taste beverage" is less than 2.
-"B": Not applicable to "A" and "C".
Figure JPOXMLDOC01-appb-T000001
 
Figure JPOXMLDOC01-appb-T000002
 
Figure JPOXMLDOC01-appb-T000001
 
Figure JPOXMLDOC01-appb-T000002
 
 表1に記載された実施例1~6および比較例1~2では、乳酸とリン酸の含有量以外は同じ条件で製造した飲料であった。これらの官能評価から、乳酸およびリン酸の含有量が一定の範囲内であれば、ビールテイスト飲料に不適な酸味を抑えられることがわかった。
 表2に記載された実施例7~12および比較例3~7では、乳酸およびリン酸の含有量は同じで、発酵条件、エタノール、HS、ジアセチル等の含有量を変えた飲料であった。これらの官能評価から、エタノール、HS、ジアセチルおよび酢酸エチルの含有量 ならびに、最終発酵度(LA)が一定の範囲内であれば、ビールテイスト飲料らしい発酵感を有し、ビールテイスト飲料らしくない甘みを抑えられることがわかった。実施例8~10、比較例3および比較例4ではHSを検出せず、その含有量は1質量ppb以下であった。
 
In Examples 1 to 6 and Comparative Examples 1 and 2 shown in Table 1, the beverages were produced under the same conditions except for the contents of lactic acid and phosphoric acid. From these sensory evaluations, it was found that if the contents of lactic acid and phosphoric acid are within a certain range, the acidity unsuitable for beer-taste beverages can be suppressed.
In been Examples 7-12 and Comparative Examples 3-7 in Table 2, the content of lactic acid and phosphoric acid are the same, there by fermentation conditions, ethanol, H 2 S, beverages with varying content of diacetyl, etc. rice field. These sensory evaluation, ethanol, H 2 S, the content of diacetyl and ethyl acetate and, if the final degree of fermentation (LA) is within a certain range, has a fermentation feeling seems low-alcohol beer, Rashiku low-alcohol beer It turned out that the sweetness was not suppressed. Examples 8-10 do not detect H 2 S in Comparative Example 3 and Comparative Example 4, the content was not more than 1 mass ppb.

Claims (12)

  1.  ジアセチルを0.07質量ppm未満、酢酸エチルを0.07質量ppm以上、および、エタノールを0.4(v/v)%以上1.5(v/v)%未満の量で含有し、
     HSの含有量が5質量ppb未満であり、
     最終発酵度(LA)が60%以下である、ビールテイスト飲料。
    Diacetyl is contained in an amount of less than 0.07 mass ppm, ethyl acetate is contained in an amount of 0.07 mass ppm or more, and ethanol is contained in an amount of 0.4 (v / v)% or more and less than 1.5 (v / v)%.
    The content of H 2 S is less than 5 mass ppb,
    A beer-taste beverage with a final fermentation degree (LA) of 60% or less.
  2.  pHが4.0未満である、請求項1に記載のビールテイスト飲料。 The beer-taste beverage according to claim 1, wherein the pH is less than 4.0.
  3.  乳酸を50~1000質量ppm、または、リン酸を50~1000ppm含有する、請求項1または2に記載のビールテイスト飲料。 The beer-taste beverage according to claim 1 or 2, which contains 50 to 1000 ppm by mass of lactic acid or 50 to 1000 ppm of phosphoric acid.
  4.  リアルエキス濃度が1~10(w/w)%である、請求項1~4のいずれかに記載のビールテイスト飲料。 The beer-taste beverage according to any one of claims 1 to 4, wherein the real extract concentration is 1 to 10 (w / w)%.
  5.  麦芽と水を含む原材料から麦汁を製造する工程、
     麦汁に酵母を添加して発酵させる工程、および
     発酵を途中で停止させる工程を有し、
     酵母の添加量が40×10cells/mL以上であり、
     発酵温度が0.6~6.0℃である、
     ビールテイスト飲料の製造方法。
    The process of producing wort from raw materials containing malt and water,
    It has a process of adding yeast to wort and fermenting it, and a process of stopping fermentation in the middle.
    The amount of yeast added is 40 × 10 6 cells / mL or more,
    Fermentation temperature is 0.6-6.0 ° C,
    How to make beer-taste beverages.
  6.  発酵工程の発酵時間が25時間以上である、請求項5に記載の製造方法 The production method according to claim 5, wherein the fermentation time in the fermentation step is 25 hours or more.
  7.  前記ビールテイスト飲料の最終発酵度(LA)が50%以下である、請求項5または6に記載の製造方法。 The production method according to claim 5 or 6, wherein the final fermentation degree (LA) of the beer-taste beverage is 50% or less.
  8.  ホップを添加する工程をさらに含む、請求項5~7のいずれかに記載の製造方法。 The production method according to any one of claims 5 to 7, further comprising a step of adding hops.
  9.  醸造酢を添加する工程をさらに含む、請求項5~8のいずれかに記載の製造方法。 The production method according to any one of claims 5 to 8, further comprising a step of adding brewed vinegar.
  10.  前記酵母が下面発酵酵母である、請求項5~9のいずれかに記載の製造方法。 The production method according to any one of claims 5 to 9, wherein the yeast is a bottom-fermenting yeast.
  11.  前記ビールテイスト飲料が、HSを5質量ppb未満、ジアセチルを0.07質量ppm未満、酢酸エチルを0.07質量ppm以上、および、エタノールを0.4(v/v)%以上1.5(v/v)%未満の量で含有する、請求項5~10のいずれかに記載の製造方法。 The low-alcohol beer is less than 5 ppb by weight of H 2 S, diacetyl less than 0.07 ppm by weight, ethyl acetate 0.07 mass ppm or more, and, ethanol 0.4 (v / v)% or more 1. The production method according to any one of claims 5 to 10, which is contained in an amount of less than 5 (v / v)%.
  12.  請求項5~11のいずれかに記載の製造方法で製造されたビールテイスト飲料。
     
    A beer-taste beverage produced by the production method according to any one of claims 5 to 11.
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JPWO2023127922A1 (en) * 2021-12-27 2023-07-06
WO2023127922A1 (en) * 2021-12-27 2023-07-06 サントリーホールディングス株式会社 Beer-flavored beverage
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