WO2014094338A1 - Procédé et boitier de détection permettant de détecter un hème libre d'exsudat de rhinopharynx - Google Patents

Procédé et boitier de détection permettant de détecter un hème libre d'exsudat de rhinopharynx Download PDF

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Publication number
WO2014094338A1
WO2014094338A1 PCT/CN2013/000030 CN2013000030W WO2014094338A1 WO 2014094338 A1 WO2014094338 A1 WO 2014094338A1 CN 2013000030 W CN2013000030 W CN 2013000030W WO 2014094338 A1 WO2014094338 A1 WO 2014094338A1
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WO
WIPO (PCT)
Prior art keywords
storage area
sampler
test
sample cup
box
Prior art date
Application number
PCT/CN2013/000030
Other languages
English (en)
Chinese (zh)
Inventor
温鑫
崔子伦
杜晓丽
Original Assignee
青岛贝尔奥生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 青岛贝尔奥生物科技有限公司 filed Critical 青岛贝尔奥生物科技有限公司
Publication of WO2014094338A1 publication Critical patent/WO2014094338A1/fr

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/726—Devices

Definitions

  • the present invention relates to a detection method, and in particular to a method for detecting free hemoglobin in the nasopharynx, and the present invention also relates to a special detection kit for detecting free hemoglobin in the nasopharynx.
  • Free hemoglobin in the nasopharynx exudate is often caused by the release of free hemoglobin from cytochromes, oxygen receptors and FixL transmembrane proteins of tissue tumor cells, precipitation or tumor tissue microcirculation exudation, and its free blood redness
  • the content of the element is very small, it is invisible to the naked eye. It is generally required to be tested by advanced instruments and analytical chemistry. The cost is high and the cycle is long. Therefore, a simple and low-cost detection method is invented to quickly determine the nasopharynx. Whether the liquid contains free hemoglobin has become an urgent problem to be solved. Book
  • the present invention provides a method for detecting free hemoglobin in the nasopharynx, and the present invention provides The method has high sensitivity and low cost, and can quickly detect free hemoglobin in the nasopharynx exudate; and the invention also provides a special detection box for detecting free hemoglobin in the nasopharynx exudate.
  • the method for detecting free hemoglobin in the nasopharynx exudate provided by the invention comprises the following detection steps -
  • sample cup Fold the sampler from the broken part of the sampler, and put the collection head with liquid exudation into the sample cup.
  • the sample cup is pre-loaded with trishydroxymethylaminocarbamate-hydrochloric acid buffer, and the amount of buffer is 3ml. Mixing to obtain a sample solution; preferably, the pH of the tris-hydroxymethylaminocarbamidine-hydrochloric acid buffer is 7.4;
  • the reagent A is prepared as follows: 50 mg of 3,3',5,5'-tetramethylbenzidine is dissolved in 100 ml of a pH 7.4 citrate buffer;
  • the reagent B was a 3% hydrogen peroxide solution.
  • the detection method of the permeate having a free hemoglobin content of 0.5 ng/L or more can be initially judged, and the detection method of the exudate having a free hemoglobin content of 20 ng/L or more can be used. It is determined that the exudate contains free hemoglobin, so the sensitivity of the detection method is high.
  • the present invention also provides a detection box for detecting free hemoglobin in the nasopharynx, comprising a casing and an outer casing outside the casing, wherein the outer surface of the outer casing is provided with a color swatch,
  • the box body is provided with a tweezers storage area, a sample cup storage area, a dropper storage area, a test cell and a sampler storage area which are recessed inward.
  • sampler storage area is disposed above the box body, and the sampler is installed therein, and the sampler is full length
  • the collecting head is made of a nylon flocking swab.
  • the shank is a plastic rod, and a folding structure is formed in the middle for folding the shank.
  • the connecting portion of the collecting head and the shank is provided with a broken portion made of a brittle plastic material.
  • test pool is disposed above the test box, having a diameter of 1.8 cm and a depth of 1.2 cm.
  • the dropper storage area is disposed on both sides of the test pool.
  • sample cup storage area is disposed on the left side of the test cell.
  • the scorpion storage area is disposed on the left side of the sample cup storage area.
  • a dropper is disposed in the dropper storage area, and the dropper is provided with a scale.
  • a sample cup is disposed in the sample cup storage area, and the sample cup is provided with a sealing cover, and the cup of the sample cup has a cylindrical shape.
  • the raft storage area is provided with a rafter, and the rafter is a plastic raft.
  • the detection method provided by the invention is simple and practical, low in cost, and can quickly screen a sample containing no free hemoglobin in the nasopharynx exudate, a sample containing free hemoglobin, and a sample which may contain free hemoglobin. Preparing for further examination; the minimum free hemoglobin concentration detectable by the invention is up to 0.5 ng/L, and the sensitivity is high; and the present invention also provides a sampler, which can be specially used for taking exudate in the nasopharyngeal area. At the same time, the invention also provides a detection box, which can be dedicated to Description
  • the reagents and reagents used in the detection process are simple in structure, and the entire detection step can be completed in one kit, saving space and reagents, and reducing the labor burden on the operator.
  • 1 is a schematic structural view of an outer package.
  • FIG. 2 is a schematic structural view of a casing.
  • FIG. 3 is a schematic structural view of a sampler.
  • Detection of free hemoglobin in the nasopharynx Open the outer box 1 of the test box, take the sampler from the box 3, put on the latex gloves, put it into the nasal cavity with the sampler, and go straight to the nasopharynx along the lower nasal passage.
  • the sample cup of the sample cup placement area 6 is prefilled with 3 ml of tris (hydroxymethylaminomethane-hydrochloric acid buffer) having a pH of 7.4, the sampler is broken, and the collection head 9 with the liquid permeation on the sampler is placed.
  • the two dropper are pre-loaded with reagent A and reagent B, respectively, in the test tank 8 sequentially add 1ml of sample liquid, reagent A 0.5ml, Reagent B 0.5ml, mix, observe the color change within 2 minutes, compare the color test plate 2 with the outer box of the test box to record the test result;
  • the reagent A is prepared as follows: Take 3,3',5,5' - 50 mg of tetramethylbenzidine dissolved in 100 ml of pH 4.4 citrate buffer; the reagent B It is a 3% hydrogen peroxide solution.
  • a detecting box for detecting free hemoglobin in the nasopharynx comprising a box body 3 and an outer box 1 outside the box body 3, the outer surface of the outer box 1 is provided with a color swatch 2, the box body 3: a dice storage area 5 recessed inwardly, a sample cup storage area 6, a dropper storage area 7, a test cell 8 and a sampler storage area 4, the sampler storage area 7 being disposed above the box body 3, the interior There is a sampler, the sampler has a total length of 14.5 cm, and includes a collecting head 9 and a shank 11 connected thereto; the collecting head 9 is made of a nylon flocking swab; the shank 11 is a plastic rod, in the middle a folding structure 10 for folding the shank 11 is provided Description
  • the connecting portion of the collecting head 9 and the shank 11 is provided with a breaking portion 12 made of a brittle plastic material;
  • the test cell 8 is disposed above the test box, having a diameter of 1.8 cm and a depth of 1.2 cm;
  • the dropper storage area 7 is provided at
  • the sample cup storage area 6 is disposed on the left side of the test cell 8;
  • the scorpion storage area 5 area is disposed on the left side of the sample cup storage area 6;
  • the dropper storage area 7 is provided with a dropper
  • the sample tube storage area 6 is provided with a sample cup
  • the sample cup is provided with a sealing cover
  • the cup of the sample cup is cylindrical;
  • the tweezers storage area 5 is provided with a tweezers
  • the dice are plastic crucibles.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention concerne un procédé de détection d'un hème libre d'exsudat de rhinopharynx. Le procédé comprend les étapes consistant à : procéder à un échantillonnage dans le rhinopharynx; diluer la solution d'échantillon; ajouter des réactifs de détection selon une certaine proportion; et déterminer si un hème existe en fonction du changement de couleur. La présente invention concerne en outre un boîtier de détection spécial permettant de détecter un hème libre d'exsudat de rhinopharynx, ledit boîtier de détection spécial pouvant être spécialement conçu pour contenir une cuve et des réactifs de détection utilisés dans le processus de détection. Le boîtier de détection est doté d'une structure simple, l'ensemble de l'étape de détection peut être achevée uniquement au moyen du boîtier de détection, l'espace et les réactifs sont économisés et la charge de travail de l'opérateur est réduite.
PCT/CN2013/000030 2012-12-17 2013-01-15 Procédé et boitier de détection permettant de détecter un hème libre d'exsudat de rhinopharynx WO2014094338A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2012105489068 2012-12-17
CN201210548906 2012-12-19

Publications (1)

Publication Number Publication Date
WO2014094338A1 true WO2014094338A1 (fr) 2014-06-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2013/000030 WO2014094338A1 (fr) 2012-12-17 2013-01-15 Procédé et boitier de détection permettant de détecter un hème libre d'exsudat de rhinopharynx

Country Status (2)

Country Link
CN (1) CN103267756B (fr)
WO (1) WO2014094338A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104062294A (zh) * 2014-07-11 2014-09-24 青岛千士医疗科技有限公司 一种支气管/肺上皮细胞线粒体损伤的检测方法
CN104990914B (zh) * 2015-06-02 2017-09-19 蒋春亮 一种肿瘤组织细胞原血红素定量检测试剂及其制备方法
CN104865250A (zh) * 2015-06-02 2015-08-26 蒋春亮 一种异型性组织细胞干化学分析试剂膜及其制备方法
CN104849272A (zh) * 2015-06-02 2015-08-19 蒋春亮 一种异型性组织细胞分析试剂
CN110772341A (zh) * 2019-12-11 2020-02-11 河南温迪生物科技研究院有限公司 用于实验研究的快速无创鼻粘液采样盒及其使用方法和应用
CN112858269A (zh) * 2020-12-25 2021-05-28 广东绿康医疗器械有限公司 鼻咽粘膜细胞游离血红素的检测试剂制备方法及使用方法
CN115372350A (zh) * 2022-09-02 2022-11-22 中迅优检生物科技(江苏)有限公司 一种游离血红素单检双判方法

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN2393493Y (zh) * 1999-07-16 2000-08-30 北京三早生物工程技术有限公司 肿瘤早查盒
WO2005106498A1 (fr) * 2004-04-30 2005-11-10 Enterix Pty. Limited Dispositif et procede de detection de la presence d'hemoglobine dans un echantillon biologique
WO2006062800A1 (fr) * 2004-12-04 2006-06-15 Freedom Health, Llc Anticorps monoclonaux et polyclonaux contre l'hemoglobine equine ainsi qu'appareil et procedes permettant d'utiliser ces anticorps et/ou des reactions de peroxydase dans l'identification et la localisation d'ulceres chez les chevaux

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201939387U (zh) * 2011-01-26 2011-08-24 深圳市麦瑞科林科技有限公司 一种鼻腔采样拭子
CN102928424B (zh) * 2012-10-30 2014-06-11 青岛贝尔奥生物科技有限公司 一种检测宫颈渗液血红素的方法
CN102914538B (zh) * 2012-10-30 2014-06-11 青岛贝尔奥生物科技有限公司 一种检测直肠渗液血红素的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2393493Y (zh) * 1999-07-16 2000-08-30 北京三早生物工程技术有限公司 肿瘤早查盒
WO2005106498A1 (fr) * 2004-04-30 2005-11-10 Enterix Pty. Limited Dispositif et procede de detection de la presence d'hemoglobine dans un echantillon biologique
WO2006062800A1 (fr) * 2004-12-04 2006-06-15 Freedom Health, Llc Anticorps monoclonaux et polyclonaux contre l'hemoglobine equine ainsi qu'appareil et procedes permettant d'utiliser ces anticorps et/ou des reactions de peroxydase dans l'identification et la localisation d'ulceres chez les chevaux

Non-Patent Citations (1)

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Title
DENG, XIAOQIN ET AL.: "TMB as Color Developing Agent Determining Free Hemoglobin in Plasma.", SHANGHAI JOURNAL OF MEDICAL LABORATORY SCIENCES., vol. 11, no. 1, January 1996 (1996-01-01), pages 61 *

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CN103267756A (zh) 2013-08-28
CN103267756B (zh) 2014-11-05

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