WO2013105734A1 - Composition permettant d'améliorer une peau sensible à l'acné - Google Patents

Composition permettant d'améliorer une peau sensible à l'acné Download PDF

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Publication number
WO2013105734A1
WO2013105734A1 PCT/KR2012/010408 KR2012010408W WO2013105734A1 WO 2013105734 A1 WO2013105734 A1 WO 2013105734A1 KR 2012010408 W KR2012010408 W KR 2012010408W WO 2013105734 A1 WO2013105734 A1 WO 2013105734A1
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Prior art keywords
temperata
prata
tam
strain
skin
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PCT/KR2012/010408
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English (en)
Korean (ko)
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김태완
김애정
남욱호
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(주)엠알이노베이션
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Priority to CN201280066080.XA priority Critical patent/CN104039334A/zh
Publication of WO2013105734A1 publication Critical patent/WO2013105734A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F21—LIGHTING
    • F21V—FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES OR SYSTEMS THEREOF; STRUCTURAL COMBINATIONS OF LIGHTING DEVICES WITH OTHER ARTICLES, NOT OTHERWISE PROVIDED FOR
    • F21V21/00—Supporting, suspending, or attaching arrangements for lighting devices; Hand grips
    • F21V21/10—Pendants, arms, or standards; Fixing lighting devices to pendants, arms, or standards
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F21—LIGHTING
    • F21W—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES F21K, F21L, F21S and F21V, RELATING TO USES OR APPLICATIONS OF LIGHTING DEVICES OR SYSTEMS
    • F21W2131/00—Use or application of lighting devices or systems not provided for in codes F21W2102/00-F21W2121/00
    • F21W2131/10—Outdoor lighting
    • F21W2131/103—Outdoor lighting of streets or roads

Definitions

  • the present invention relates to a composition for acne skin improvement and, more particularly picture drawer Douce Tam Prata Tam Prata (Photorhabdus temperata subsp. Temperata) relates to for acne skin improving composition containing a culture of the strain as an effective ingredient.
  • Acne is a chronic, refractory, and inflammatory skin disease that occurs in hair follicles and sebaceous glands.
  • the cause of acne begins with the abnormal action of testosterone, a male hormone in human hormones. It flows into the sebaceous glands and promotes the production of oil, which causes the excessively secreted oil to fill the sebum pores.
  • propionibacterium acnes which is a cause of acne, is difficult to select a drug because of artificial cultivation, and particularly, antibiotics to which an antibiotic is added has a problem that cannot be used as a cosmetic.
  • the antimicrobial substances produced by microorganisms have the advantage of inhibiting a wide range of microorganisms and low selectivity toxicity to higher organisms.
  • Japanese Patent Laid-Open No. 2003-259860 discloses a cosmetic composition containing lactococcus lactose and an antibacterial culture solution using the same.
  • Korean Patent No. 0536522 discloses a cosmetic composition containing Enterococcus kraftium and bacteriocin using the same.
  • cosmetic compositions using lactic acid bacteria and their cultures have disadvantages of high unit cost in the process for obtaining lactic acid bacteria metabolites and high process costs for separation and purification of metabolites.
  • An object of the present invention is photo drawer Douce Tam Prata Tam Prata (Photorhabdus temperata subsp. Temperata) of acne causing microorganisms that occur on the skin containing a culture solution of the strain as an active ingredient propionyl sludge tumefaciens inhibiting the growth of arc Ness bacteria and It is to provide a composition for acne skin improvement, safe to the skin, and excellent moisturizing effect.
  • Skin topical pharmaceutical composition to improve acne skin according to the present invention for achieving the above object is characterized in that it contains the culture medium of the picture drawer Douce Tam Prata Tam Prata (Photorhabdus temperata subsp. Temperata) strain as an effective ingredient .
  • the photo-drawer Douce culture of Tam Prata Tam Prata (Photorhabdus temperata subsp. Temperata) strain is characterized in that comprises portions 10 to 40 by weight based on the composition of 100 parts by weight .
  • the composition is characterized in that the cream, gel, patch, spray, ointment, warning, lotion, linen, or pasta.
  • a cosmetic composition to improve acne according to the invention is characterized in that it contains the culture medium of the picture drawer Douce Tam Tam Prata Prata (Photorhabdus temperata subsp. Temperata) strain as an effective ingredient.
  • the culture of the photo drawer Douce Tam Tam Prata Prata (Photorhabdus temperata subsp. Temperata) strain is characterized in that comprises 10 to 40 parts by weight based on 100 parts by weight of the composition.
  • the cosmetic is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence , Nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, face wash, soap, treatment, essence, cosmetic pack, characterized in that any one selected from the group consisting of.
  • temperata culture medium inoculated by culturing the strain in the culture medium separated in the step (a), (c ) it characterized in that it comprises the step of after removing and separating the polysaccharide from the picture drawer Douce Tam Tam Prata Prata (Photorhabdus temperata subsp. temperata) the strain is cultured broth, separating and removing the strain.
  • drawer Douce Tam Prata Tam Prata Photorhabdus temperata subsp. Temperata
  • strain culture solution in the manufacturing method (a) the separation in step is 20-40 minutes in a sodium chloride solution containing the 300ppm streptomycin After disinfection and grinding, it is characterized in that the separation using a medium.
  • picture drawer Douce Tam Prata Tam Prata (Photorhabdus temperata subsp. Temperata) thereby isolates containing the sludge acne induced microorganism Propionibacterium caused to the skin and inhibiting the growth of tumefaciens arc Ness bacteria, to the skin safety And, moisturizing effect is also provided excellent acne skin improvement composition.
  • FIG. 1 is a view showing the nucleotide sequence of the picture drawer Douce system Prata system Prata strain (Photorhabdus temperata subsp. Temperata) an active ingredient of the present invention
  • Figure 2 is a picture drawer Douce system Prata system Prata view showing a homology comparison between the strains (Photorhabdus temperata subsp. Temperata),
  • Figure 3 is a diagram showing the taxonomy of taxonomy of Photorhabdus temperata ( temperata subsp. Temperata ) as an active ingredient of the present invention.
  • the strain used as active ingredients for acne skin improving the composition of the present invention is a picture drawer Douce system Prata system Prata strain (Photorhabdus temperata subsp. Temperata).
  • This picture drawer Douce system Prata system Prata strain (Photorhabdus temperata subsp. Temperata) is usually insect as a known strain used in the immunosuppressive substance produced, in the present invention, the causative agent of acne propynyl for standing on the skin for these the strain Onibacterium acnes ( Propionibacterium acnes ) by inhibiting the growth of the acne is found to be able to inhibit the development of the acne skin improvement composition of the present invention will be used as an active ingredient.
  • the strain is characterized in that it is used in any one or more forms of the cells or culture medium of the strain, in particular when using the culture medium of the strain is first isolated from the photopathus tamprata tamprata strain from insect pathogenic nematode infection .
  • the isolated photolabdu tamprata tamprata strain is placed in an incubator to be a shaker and incubated at 250 rpm for 20 to 30 hours, preferably 25 to 24 hours, preferably 4 days. Thereafter, 2 times of 2-isopropanol was added to the culture solution and sonicated for 30 minutes to 1 hour, and then left at 2 to 6 ° C., preferably at 4 ° C. for 20 to 25 hours to separate polysaccharides from the culture solution. And remove. Next, the culture medium from which the polysaccharide is removed is centrifuged for 30 to 50 minutes and then filtered to remove the strain, and the remaining culture solution is used as an external skin pharmaceutical composition or cosmetic composition for acne skin improvement.
  • the culture medium of the strain is characterized in that it contains 10 to 40% by weight relative to the total weight of the acne skin improvement composition, when the culture medium of the strain is contained less than 10% by weight relative to the total weight of the acne skin improvement effect It is not suitable for use as a composition for improving acne skin, and when it is contained in an amount exceeding 40% by weight, acne skin improvement effect does not increase any more, but is likely to cause skin irritation.
  • the present invention provides a skin external pharmaceutical composition for improving acne skin containing a culture solution of photolabdus tamprata tamprata strain obtained by the above method as an active ingredient.
  • the extract contains 0.1 to 50% by weight based on the total weight of the composition.
  • the external dermal pharmaceutical composition comprising the extract of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.
  • compositions comprising a culture solution of the photolabdu tamprata tamprata strain according to the present invention, respectively, according to a conventional method, external preparation and sterilization of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, etc. It can be formulated and used in the form of an injectable solution, and preferably provides an external skin pharmaceutical composition of a cream, gel, patch, spray, ointment, warning, lotion, linen, pasta or cataplasma.
  • Examples of carriers, excipients, and diluents that may be included in the composition comprising the culture medium of the photoractus tamprata tamprata strain include lactose, dextrose, sucrose, oligosaccharide, sorbitol, mannitol, xylitol, erythritol, maltitol, and starch. , Acacia rubber, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil Can be mentioned.
  • composition comprising the culture solution of the photolapus tamprata tamprata strain of the present invention is a skin external preparation formulation that can be applied to the skin, such as cream, gel, patch, spray, ointment, warning agent, lotion agent, lining agent, It may be prepared and used as a pharmaceutical composition in the form of an external preparation for skin of pasta or cataplasma, but is not limited thereto.
  • composition containing the culture solution of the tamprata tamprata strain of the present invention can be used in various ways, such as cosmetics and face wash for skin whitening and wrinkle improvement effect.
  • products to which the composition can be added include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nourishing lotion, massage cream, nourishing cream, moisturizing cream, hand cream, foundation , Essences, nutrition essences, packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers, such as face washes, soaps, treatments, essences, beauty packs.
  • Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.
  • the water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like.
  • Their salts thiamine hydrochloride, sodium ascorbate salt, etc.
  • derivatives ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.
  • the water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.
  • the oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.
  • the polymer peptide may be any compound as long as it can be incorporated into cosmetics.
  • collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given.
  • Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.
  • the polymer polysaccharide may be any compound as long as it can be blended into cosmetics.
  • hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned.
  • chondroitin sulfate or its salt can be normally purified from a mammal or fish.
  • the sphingolipid may be any compound as long as it can be blended into cosmetics.
  • ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned.
  • Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.
  • the seaweed extract may be any compound as long as it can be blended into cosmetics.
  • the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like.
  • calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention.
  • Seaweed extract can be obtained by purification from seaweed by conventional methods.
  • Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.
  • Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.
  • ester fats and oils As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, tri
  • Hydrocarbon-based fats and oils such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.
  • silicone-based oils and fats examples include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl.
  • Modified silicone oil amino modified silicone oil and the like.
  • Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.
  • Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.
  • the moisturizing agent examples include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.
  • fat-soluble low molecular humectants examples include cholesterol and cholesterol esters.
  • water-soluble polymer examples include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.
  • fat-soluble polymers examples include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.
  • emollient examples include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.
  • nonionic surfactant nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.
  • nonionic surfactant self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.
  • Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, and alkylamide phosphates.
  • Alkyloylalkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfo acetate, acyl hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. are mentioned. .
  • amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type.
  • An amphoteric surfactant etc. are mentioned.
  • Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide
  • Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorgan
  • Metal soaps such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glyc
  • Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid.
  • paraaminobenzoic acid ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid.
  • fungicides hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.
  • antioxidants examples include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.
  • pH adjuster examples include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.
  • Examples of the alcohol include higher alcohols such as cetyl alcohol.
  • the compounding component which may be added other than this is not limited to this, Moreover, although all said components can be mix
  • the cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.
  • Components included in the cosmetic composition of the present invention as an active ingredient may include components commonly used in cosmetic compositions in addition to the culture medium of the tamprata tamprata strain, for example, stabilizers, solubilizers, vitamins, Conventional adjuvants and carriers such as pigments and fragrances.
  • the functional cosmetic composition for acne improvement of the present invention may be prepared in any formulation commonly prepared in the art, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetics and the like. .
  • the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.
  • the formulation of the present invention is a paste, cream or gel
  • animal carriers vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc.
  • carrier components can be used as carrier components.
  • lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.
  • a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.
  • liquid carrier diluents such as water, ethanol or propylene glycol
  • suspension agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.
  • the carrier component is aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide.
  • Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.
  • composition for improving acne skin of the present invention it is possible to produce and provide various types of cosmetics effective for acne skin improvement as described above.
  • Heterorhaduitis bacteriophora infections 500 or more were surface sterilized in 0.06% NaCl solution containing 300 ppm streptomycin for 30 minutes, and then the whole nematode was ground using a homogenizer. Using a MacConkey agar medium of 1 to fluoresce the light pink strain was isolated.
  • the isolated strain appeared as shown in FIG. 1, and in FIG. 2, Photorhabdus temperata. subsp. As a result of comparing the homology with temperata showed 100% homology, as shown in Fig. 3, the isolated strain was identified as Photorhabdus temperata subsp. temperata ).
  • 5% YS medium was used as the basic medium, and its composition was cooking oil, egg yolk powder, cholesterol, lard, lecithin, whole milk powder, sodium chloride (NaCl), monobasic potassium phosphate, and yeast extract (Yeast extract) was used.
  • Example 3 TABLE 3 ingredient Content (% by weight) Strain Culture of Example 2
  • Example 4 Example 5
  • Example 6 Comparative Example 1 10
  • 20 30
  • Phenolicethanol 1.2
  • Stearic acid 1.5
  • Beeswax 1.0 Cocoglucoside
  • Polyquaternium-10 1.7
  • Sorbitan stearate 0.5
  • Squalene 3.0 Cured Vegetable Oil
  • 1.0 Sodium Magnesium Silicate 0.1 Betaine 3.0
  • Triethanol amine 1.0 incense 0.01 Distilled water Remaining amount Sum 100
  • the growth inhibitory effect on the acne strain according to the content was tested using the photolabdus tamprata tamprata strain culture solution obtained in Example 2.
  • the inoculated solid medium was placed in an anaerobic incubator and incubated at 35 ° C. for 5 days, and the clear zone was observed.
  • the results are shown in Table 4 below.
  • Ersthromycin (0.2%) was used as a control.
  • the cosmetic formulations of Examples 3 to 6 were prepared to test other stability to temperature changes of the cosmetic formulation.
  • the cosmetic formulations of Examples 3 to 6 contain the photolabduus tempprata species Tempprata strain culture solution according to the content of Example 2, and the rest of the components were used as a composition commonly used in the art. It was.
  • the cosmetic formulation and the control example 1 of Examples 3 to 6 of the present invention prepared as described above were stored in a constant temperature water bath kept at constant temperature at 50 ° C. in a glass container for 2 weeks, and then placed in an opaque glass container at 0 ° C. After keeping for a week and kept in a refrigerator completely blocked state for a week and then the degree of discoloration was measured according to the product discoloration evaluation criteria of Table 5 below.
  • Example 6 Temperature Discoloration of the test substance Temperature Example 3
  • Example 4 Example 5
  • Example 6 Comparative Example 1 50 °C 0 0 0 0 0 0 0 °C 0 0 0 0 0 0 0 0
  • Control Example 1 containing 0.2% by weight of erythromycin was tested together.
  • test site was wiped with 70% ethanol and dried, and the prepared test substance was applied to the inner part of the forearm of the tester by 20 ⁇ g.
  • test site was marked with a marker pen, and the test site was observed using a magnifying glass to observe erythema and edema.
  • Comparative Example 1 containing 0.2% eromycin has low skin stability, it contained the Photolabdus tempprata species tempprata culture solution of Example 3 of the present invention.
  • the formulation was shown to be safe for the human skin, indicating that it is safe to use.
  • Example 3 and Comparative Example 1 were randomly divided into two sets of 50 people each, and applied to the face and front ends five times daily for two months to measure changes in the amount of water in the skin.
  • the change in skin moisture content is measured by measuring the conduction of the skin in advance using a coneometer under constant temperature and humidity conditions (temperature 20 °C, humidity 50%) before applying the formulations of Example 3 and Comparative Example 1.
  • the reaction after elapsed was measured on a weekly basis, and evaluated.
  • a composition for acne skin to improve the invention the picture drawer Douce Tam Prata species Tam Prata (Photorhabdus temperata subsp. Temperata) acne induced microorganism Propionibacterium sludge bacterium growth of Solarium arc Ness bacteria generated in the skin by containing the strain It is to be able to provide a composition for acne skin improvement that inhibits, is safe for the skin, and also excellent moisturizing effect.
  • Tam Prata Photorhabdus temperata subsp. Temperata
  • acne induced microorganism Propionibacterium sludge bacterium growth of Solarium arc Ness bacteria generated in the skin by containing the strain
  • the present invention is applied to the acne skin improvement composition technology for improving acne skin.

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  • Cosmetics (AREA)

Abstract

La présente invention concerne une composition permettant d'améliorer une peau sensible à l'acné, contenant une culture en solution de Photorhabdus temperata subsp. temperat comme principe actif. La présente invention concerne une composition permettant d'améliorer une peau sensible à l'acné, qui inhibe la croissance de Propionibacterium acnes, un microorganisme provoquant l'acné dans la peau, qui contient une culture en solution de Photorhabdus temperata subsp. temperat, qui est sûre pour la peau et qui présente des effets hydratants excellents.
PCT/KR2012/010408 2012-01-10 2012-12-04 Composition permettant d'améliorer une peau sensible à l'acné WO2013105734A1 (fr)

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CN201280066080.XA CN104039334A (zh) 2012-01-10 2012-12-04 痤疮皮肤改善用组合物

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KR1020120002844A KR101151861B1 (ko) 2012-01-10 2012-01-10 여드름 피부개선용 조성물
KR10-2012-0002844 2012-01-10

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KR102174525B1 (ko) * 2019-09-17 2020-11-05 코스맥스 주식회사 신규한 판토에아 왈리시 루미테리아 균주 및 그 균주 배양액을 포함하는 피부 미용 개선용 조성물

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KR20040104872A (ko) * 2003-06-04 2004-12-13 김용균 포토랍두스 템페라타 템페라타 ａｎｕ101
KR20100007496A (ko) * 2008-07-14 2010-01-22 안동대학교 산학협력단 곤충병원선충 슈타이너네마 몬티콜럼에서 유래된곤충병원세균 세라티아 ｓｐ. ＡＮＵ101
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KR20040104872A (ko) * 2003-06-04 2004-12-13 김용균 포토랍두스 템페라타 템페라타 ａｎｕ101
KR20100007496A (ko) * 2008-07-14 2010-01-22 안동대학교 산학협력단 곤충병원선충 슈타이너네마 몬티콜럼에서 유래된곤충병원세균 세라티아 ｓｐ. ＡＮＵ101
KR20110138110A (ko) * 2010-06-18 2011-12-26 안동대학교 산학협력단 곤충병원세균으로부터 분리한 새로운 살충활성 물질 및 이의 용도

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KR101151861B1 (ko) 2012-06-08

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