WO2012141542A3 - 미생물 발효를 통해 제조된 생성물을 흡착제를 이용하여 분리 정제하는 장치 및 방법 - Google Patents

미생물 발효를 통해 제조된 생성물을 흡착제를 이용하여 분리 정제하는 장치 및 방법 Download PDF

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WO2012141542A3
WO2012141542A3 PCT/KR2012/002841 KR2012002841W WO2012141542A3 WO 2012141542 A3 WO2012141542 A3 WO 2012141542A3 KR 2012002841 W KR2012002841 W KR 2012002841W WO 2012141542 A3 WO2012141542 A3 WO 2012141542A3
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refining
separating
adsorbent
fermentation
product manufactured
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PCT/KR2012/002841
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English (en)
French (fr)
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WO2012141542A2 (ko
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이상현
엄문호
이율리아
전상준
조정희
최진달래
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지에스칼텍스(주)
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Priority to US14/111,100 priority Critical patent/US10150973B2/en
Publication of WO2012141542A2 publication Critical patent/WO2012141542A2/ko
Publication of WO2012141542A3 publication Critical patent/WO2012141542A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C12M1/06—Apparatus for enzymology or microbiology with gas introduction means with agitator, e.g. impeller
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    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/12—Bioreactors or fermenters specially adapted for specific uses for producing fuels or solvents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C12M25/18—Fixed or packed bed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C12M25/20—Fluidized bed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C12P7/28—Acetone-containing products
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1885—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in parallel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Sustainable Development (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
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  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 미생물 배양을 통하여 생산한 생성물의 발효 분리 정제 장치 및 발효 분리 정제 방법에 대한 것이다. 본 발명의 발효 분리 정제 장치 및 발효 분리 정제 방법은 미생물 배양을 통하여 생산한 생성물을 간편하고 연속적이면서도 높은 효율로 분리 정제할 수 있다.
PCT/KR2012/002841 2011-04-14 2012-04-13 미생물 발효를 통해 제조된 생성물을 흡착제를 이용하여 분리 정제하는 장치 및 방법 WO2012141542A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US14/111,100 US10150973B2 (en) 2011-04-14 2012-04-13 Apparatus and method for separating and refining product manufactured by microbial fermentation by using adsorbent

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20110034883 2011-04-14
KR10-2011-0034883 2011-04-14

Publications (2)

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WO2012141542A2 WO2012141542A2 (ko) 2012-10-18
WO2012141542A3 true WO2012141542A3 (ko) 2013-03-07

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PCT/KR2012/002845 WO2012141546A2 (ko) 2011-04-14 2012-04-13 미생물 발효를 통해 제조된 생성물을 흡착제를 이용하여 분리 정제하는 장치 및 방법
PCT/KR2012/002841 WO2012141542A2 (ko) 2011-04-14 2012-04-13 미생물 발효를 통해 제조된 생성물을 흡착제를 이용하여 분리 정제하는 장치 및 방법

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US (2) US10150973B2 (ko)
EP (1) EP2698423B1 (ko)
KR (2) KR101487057B1 (ko)
CN (1) CN103476918B (ko)
AU (1) AU2012243504B2 (ko)
BR (1) BR112013026238B1 (ko)
CA (1) CA2832960C (ko)
MY (1) MY180565A (ko)
WO (2) WO2012141546A2 (ko)

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CN104974013B (zh) * 2015-05-28 2017-03-15 南京工业大学 一种利用连续色谱技术分离丁醇发酵液的工艺
CN107446813B (zh) * 2017-08-22 2023-10-31 江西省科学院微生物研究所 一种连续转化生产2-pe的装置及其连续转化生产2-pe的方法
CN107583308B (zh) * 2017-10-19 2023-10-10 苏州苏震生物工程有限公司 一种用于1,3-丙二醇和2,3-丁二醇纯化的设备及工艺
KR101989814B1 (ko) 2017-11-28 2019-06-17 지에스칼텍스 주식회사 클로스트리디움 및 대장균에서 복제가 가능한 셔틀 플라스미드 및 이를 이용하여 제조된, 오탄당 대사 및 발효 성능이 증강된 재조합 미생물
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WO2012141546A2 (ko) 2012-10-18
AU2012243504A1 (en) 2013-10-31
KR20120117690A (ko) 2012-10-24
KR101487057B1 (ko) 2015-01-27
US20140038250A1 (en) 2014-02-06
AU2012243504B2 (en) 2015-08-13
EP2698423B1 (en) 2020-12-23
CA2832960C (en) 2020-07-21
EP2698423A2 (en) 2014-02-19
CN103476918A (zh) 2013-12-25
EP2698423A4 (en) 2014-12-10
BR112013026238B1 (pt) 2020-02-11
MY180565A (en) 2020-12-02
KR20120117689A (ko) 2012-10-24
WO2012141546A3 (ko) 2013-03-07
WO2012141542A2 (ko) 2012-10-18
BR112013026238A2 (pt) 2017-06-20
CA2832960A1 (en) 2012-10-18
US20140030777A1 (en) 2014-01-30
KR101418638B1 (ko) 2014-07-10
CN103476918B (zh) 2016-09-14
US10150973B2 (en) 2018-12-11

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