WO2012023391A1 - 非特異吸着の精製機構を備えたspfs用センサ - Google Patents
非特異吸着の精製機構を備えたspfs用センサ Download PDFInfo
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- WO2012023391A1 WO2012023391A1 PCT/JP2011/067085 JP2011067085W WO2012023391A1 WO 2012023391 A1 WO2012023391 A1 WO 2012023391A1 JP 2011067085 W JP2011067085 W JP 2011067085W WO 2012023391 A1 WO2012023391 A1 WO 2012023391A1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N21/05—Flow-through cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Definitions
- the present invention relates to a combination of a purification area and a sensor area arranged in series in a flow path for surface plasmon excitation enhanced fluorescence spectroscopy (SPFS; Surface Plasmon-field enhanced Fluorescence Spectroscopy).
- SPFS surface plasmon excitation enhanced fluorescence spectroscopy
- SPFS means that the irradiated laser light has a rough wave (surface plasmon) on the surface of the metal thin film in contact with the dielectric under the condition that the irradiated laser light is attenuated by total reflection [ATR] on the surface of the metal thin film.
- ATR total reflection
- SPFS is a system that detects a very small concentration region, it has an essential problem that a decrease in assay signal and a further increase in assay blank lead to a decrease in detection sensitivity. Furthermore, in SPFS, when non-specific adsorption at a level that causes a resonance angle shift occurs, there is a serious problem that the electric field enhancement intensity changes.
- Patent Document 1 discloses a biosensor for measuring SPR (SPR: Surface Plasmon Resonance) composed of a substrate having at least a surface to which a physiologically active substance is bound and a surface (reference part) that does not hold the physiologically active substance.
- the reference part is protected by two or more hydrophilic groups. As a result, non-specific adsorption to the reference portion is attempted to be suppressed.
- Patent Document 2 SPR measurement in which two regions, a measurement region where a ligand is fixed and a reference region where a ligand is not fixed, are arranged in parallel along the flow direction of the analyte.
- a sensor is disclosed. The difference between the measurement signal detected from the measurement area and the reference signal detected from the reference area makes it possible to obtain measurement data in which individual differences between different sensors are canceled.
- an object of the present invention is to provide a sensor area that can suppress a decrease in assay signal and an increase in assay blank in SPFS measurement.
- the present inventors have found that the nonspecific adsorption component contained in the specimen can be removed in advance by contacting with the specimen purification area before contacting with the sensor area.
- the headline and the present invention were completed.
- the SPFS sensor chip of the present invention is characterized in that, in the surface plasmon excitation enhanced fluorescence spectroscopy [SPFS] measurement flow path, a purification area is arranged upstream with respect to the flow direction, and a sensor area is arranged downstream thereof.
- SPFS surface plasmon excitation enhanced fluorescence spectroscopy
- the sensor area includes: a transparent support; a metal thin film formed on one surface of the support; a self-organized single film formed on the other surface of the metal thin film that is not in contact with the support.
- a molecular film [SAM]; a hydrophilic polymer layer formed on the other surface of the SAM which is not in contact with the metal thin film; and a hydrophilic polymer layer which is not in contact with the SAM of the hydrophilic polymer layer It is preferably formed on the surface of a plasmon excitation sensor comprising: a physiologically active substance immobilized on one surface.
- the purification area preferably includes a specimen purification area, the specimen purification area comprising: a transparent support; a metal thin film formed on one surface of the support; and the support of the metal thin film.
- the purification area preferably includes the sample purification area and the labeled antibody purification area, and the labeled antibody purification area is preferably formed on the surface of the plasmon excitation sensor.
- the hydrophilic polymer constituting the hydrophilic polymer layer contained in the sensor substrate is preferably different from the hydrophilic polymer constituting the hydrophilic polymer layer contained in the plasmon excitation sensor.
- the purification area may be arranged in all the channels upstream from the sensor area.
- the assay method of the present invention is characterized by including at least the following steps (a) to (e).
- step (x) Furthermore, it is preferable to include the following step (x).
- the present invention can provide a combination of a purification area and a sensor area that can suppress a decrease in assay signal and an increase in assay blank in SPFS measurement.
- the present invention can purify a mixture of a ligand / contaminant conjugate and a fluorescent dye to increase the purity of the conjugate of the ligand and the fluorescent dye, thereby further reducing the assay signal and raising the assay blank.
- a combination of a purification area and a sensor area can be provided.
- FIG. 1 shows the steps (a) and (b) included in the assay method of the present invention, that is, the sample purification area (30) arranged upstream in the flow direction in the SPFS measurement channel (10).
- the nonspecific adsorption substance (22,23) contained in the specimen (20) is removed, and the purified specimen (40) (that is, the specimen enriched with the analyte (21)) reacts with the sensor area (50).
- FIG. 2 shows that the labeled antibody solution (60) is brought into contact with the labeled antibody purification area (70) in the step (x) that is preferably included in the assay method of the present invention, that is, the SPFS measurement channel (10).
- FIG. FIG. 3 shows a transparent support, a metal thin film formed on one surface of the support, and a self-assembled monomolecule formed on the other surface of the metal thin film that is not in contact with the support.
- a substrate (corresponding to "SAM” in the graph) comprising a film [SAM] and CMD (carboxymethyl dextran) formed on the other surface of the SAM contained in the substrate that is not in contact with the metal thin film
- SAM film [SAM] and CMD (carboxymethyl dextran) formed on the other surface of the SAM contained in the substrate that is not in contact with the metal thin film
- CMD carboxymethyl dextran
- FIG. 4 shows that when plasma flows on the sensor substrate, some plasma component is adsorbed on the sensor substrate, so that the resonance angle shifts by about 1 ° compared to when the buffer flows on the sensor substrate.
- FIG. 5 is a graph showing the assay signals obtained from the results of Example 1 and Comparative Example 1, respectively.
- FIG. 6 is a graph showing blank signals obtained from the results of Example 2 and Comparative Example 2 as bar graphs.
- the SPFS sensor chip of the present invention is characterized in that, in the surface plasmon excitation enhanced fluorescence spectroscopy [SPFS] measurement flow path, a purification area is arranged upstream with respect to the flow direction, and a sensor area is arranged downstream thereof. . That is, the SPFS sensor chip has a refinement area and a sensor area arranged in series with respect to the flow direction.
- SPFS surface plasmon excitation enhanced fluorescence spectroscopy
- the sensor area used in the present invention was formed on a transparent support; a metal thin film formed on one surface of the support; and the other surface of the metal thin film not in contact with the support A self-assembled monolayer [SAM]; a hydrophilic polymer layer formed on the other surface of the SAM not in contact with the metal thin film; and the SAM of the hydrophilic polymer layer And a biologically active substance immobilized on the other surface not in contact with the surface of the plasmon excitation sensor comprising: a purified specimen (40) as shown in FIG. That is, the area where concentrated analyte is captured and subjected to SPFS measurement.
- the “transparent support” used in the present invention may be made of quartz or glass, or may be made of plastic such as polycarbonate [PC] or cycloolefin polymer [COP], and has a refractive index [nd].
- the size (length ⁇ width) is not particularly limited as long as the thickness is preferably 1.40 to 2.20 and the thickness is preferably 0.01 to 10 mm, more preferably 0.5 to 5 mm.
- the transparent support made of glass is commercially available from “BK7” (refractive index [nd] 1.52) and “LaSFN9” (refractive index [nd] 1.85), ( “K-PSFn3” (refractive index [nd] 1.84), “K-LaSFn17” (refractive index [nd] 1.88) and “K-LaSFn22” (refractive index [nd]) manufactured by Sumita Optical Glass Co., Ltd. 1.90) and “S-LAL10” (refractive index [nd] 1.72) manufactured by OHARA INC. Are preferred from the viewpoints of optical properties and detergency.
- the transparent support is preferably cleaned with acid and / or plasma before forming a metal thin film on the surface.
- the cleaning treatment with acid it is preferable to immerse in 0.001 to 1N hydrochloric acid for 1 to 3 hours.
- Examples of the plasma cleaning treatment include a method of immersing in a plasma dry cleaner (“PDC200” manufactured by Yamato Scientific Co., Ltd.) for 0.1 to 30 minutes.
- a plasma dry cleaner (“PDC200” manufactured by Yamato Scientific Co., Ltd.) for 0.1 to 30 minutes.
- the “metal thin film” formed on one surface of the transparent support is preferably made of at least one metal selected from the group consisting of gold, silver, aluminum, copper, and platinum, more preferably gold. It is desirable to consist of these, and the alloy of these metals may be sufficient. Such metal species are preferable because they are stable against oxidation and increase in electric field due to surface plasmons increases.
- a thin film of chromium, nickel chromium alloy or titanium in advance because glass and a metal thin film can be bonded more firmly only when a glass flat substrate is used as the transparent support.
- Examples of methods for forming a metal thin film on a transparent support include sputtering, vapor deposition (resistance heating vapor deposition, electron beam vapor deposition, etc.), electrolytic plating, electroless plating, and the like. Since it is easy to adjust the thin film formation conditions, it is preferable to form a chromium thin film and / or a metal thin film by sputtering or vapor deposition.
- the thickness of the metal thin film is preferably gold: 5 to 500 nm, silver: 5 to 500 nm, aluminum: 5 to 500 nm, copper: 5 to 500 nm, platinum: 5 to 500 nm, and alloys thereof: 5 to 500 nm.
- the thickness of the thin film is preferably 1 to 20 nm.
- gold 20-70 nm
- silver 20-70 nm
- aluminum 10-50 nm
- copper 20-70 nm
- platinum 20-70 nm
- alloys thereof 10-70 nm
- chromium The thickness of the thin film is more preferably 1 to 3 nm.
- the thickness of the metal thin film is within the above range because surface plasmons are easily generated. Moreover, if it is a metal thin film which has such thickness, a magnitude
- SAM Self-Assembled Monolayer
- the SAM contains a carboxyalkanethiol having about 4 to 20 carbon atoms (for example, available from Dojindo Laboratories Co., Ltd., Sigma Aldrich Japan Co., Ltd.), in particular.
- a carboxyalkanethiol having about 4 to 20 carbon atoms for example, available from Dojindo Laboratories Co., Ltd., Sigma Aldrich Japan Co., Ltd.
- 10-carboxy-1-decanethiol is used.
- Carboxyalkanethiol having 4 to 20 carbon atoms has properties such as little optical influence of SAM formed using it, that is, high transparency, low refractive index, and thin film thickness. Therefore, it is preferable.
- the SAM formation method is not particularly limited, and a conventionally known method can be used.
- a method of immersing a glass transparent support having a metal thin film formed on an ethanol solution containing 10-carboxy-1-decanethiol manufactured by Dojindo Laboratories. It is done.
- the thiol group of 10-carboxy-1-decanethiol binds to and is immobilized on the metal, and self-assembles on the surface of the metal thin film to form a SAM.
- the hydrophilic polymer layer is preferably provided so as to intervene the bond between the SAM and the physiologically active substance.
- the hydrophilic polymer constituting the hydrophilic polymer layer is preferably dextran such as carboxymethyldextran [CMD] as the polysaccharide and polyacrylic acid as the synthetic resin.
- hydrophilic polymer layer on the SAM surface for example, 1- (3-dimethylaminopropyl) -3ethylcarbodiimide [EDC] alone, which is a water-soluble carbodiimide, or EDC and N-hydroxysuccinimide [NHS] is used.
- EDC 1- (3-dimethylaminopropyl) -3ethylcarbodiimide
- NHS N-hydroxysuccinimide
- the physiologically active substance may be a protein that specifically binds to the substance to be captured, such as an enzyme that specifically binds to an antigen, an enzyme for a substrate / coenzyme, a receptor for a hormone, or a protein A for an antibody.
- an enzyme that specifically binds to an antigen
- an enzyme for a substrate / coenzyme such as an enzyme for a substrate / coenzyme, a receptor for a hormone, or a protein A for an antibody.
- -Protein G avidins for biotin, calmodulin for calcium, and the like.
- an antibody is preferable as the "protein that specifically binds to the target substance to be captured”.
- the method for immobilizing a physiologically active substance is a known method, for example, by activating the carboxyl group of SAM or hydrophilic polymer by EDC alone or in combination with EDC and immobilizing the physiologically active substance having an amino group.
- the method of making it is not limited to these as long as the protein is not inactivated.
- the purification area preferably includes a sample purification area, and more preferably includes both a sample purification area and a labeled antibody purification area.
- the purification area is preferably arranged in all the upstream channels from the sensor area.
- the sample purification area is a non-specific adsorption substance other than the analyte contained in the sample, that is, an area that non-specifically adsorbs and attaches so-called impurities, and the sample that has passed through this purification area is a part of the impurities ( Preferably all) have been removed.
- This non-specific adsorbent includes, for example, albumin and the like, and albumin is preferentially captured in the sample purification area.
- the specimen purification area comprises: a transparent support; a metal thin film formed on one surface of the support; a self-organization formed on the other surface of the metal thin film that is not in contact with the support A monomolecular film [SAM]; and a hydrophilic polymer layer formed on the other surface of the SAM that is not in contact with the metal thin film; and formed on the surface of a sensor substrate.
- SAM monomolecular film
- the “sensor substrate” (sample purification area) has the same configuration as the “plasmon excitation sensor” (sensor area) that does not immobilize the physiologically active substance on the hydrophilic polymer layer, and immobilizes the physiologically active substance. Except for this, it can be produced by the same method as the plasmon excitation sensor.
- hydrophilic polymer constituting the hydrophilic polymer layer included in the “plasmon excitation sensor” sensor area and labeled antibody purification area
- high hydrophilicity included in the “sensor substrate” sample purification area
- the hydrophilic polymer constituting the molecular layer may be different or the same. If they are different, more kinds of contaminants contained in the sample can be adsorbed and attached non-specifically, and as a result, the decrease in assay signal and the increase in assay blank can be further suppressed. Therefore, it is preferable.
- the purified area for labeled antibody is a conjugate in which a fluorescent dye is labeled on a ligand such as an antibody or various contaminants (non-specifically adsorbed substances / antibodies that do not recognize an analyte even if they are antibodies) that are considered to be contained in trace amounts.
- the area of the mixture of gates that is, the non-specifically adsorbed / adhered conjugate of the contaminant and the fluorescent dye in the mixture of the conjugate of the ligand and the fluorescent dye, and the conjugate of the contaminant and the fluorescent dye.
- Such a purified area for labeled antibody is formed on the surface of the “plasmon excitation sensor”, that is, the purified area for labeled antibody and the sensor area preferably have the same configuration. If the labeled antibody purification area and the sensor area have the same configuration, the labeled antibody solution (60) that has passed through the labeled antibody purification area (70) having the same configuration as the sensor area (50), as shown in FIG.
- the labeled antibody purification area can be prepared in the same manner as the sensor area.
- the flow path may be a Y-shaped (form in which the sample purification area and the labeled antibody purification area are divided into two branches and merged into the sensor area),
- the sensor substrate purification area for specimen
- the sensor substrate is removed from the flow path, and the plasmon excitation sensor (purification area for labeled antibody) is installed at the same location. Also good.
- the purified labeled antibody is detected by the sensor.
- a mode of passing through the area is preferable.
- the assay method of the present invention includes at least the following steps (a) to (e), and preferably includes a washing step and further the following step (x).
- Cleaning step cleaning the surface of the plasmon excitation sensor obtained through the step (b) and / or the surface of the plasmon excitation sensor obtained through the step (c), and step (x): Contacting the conjugate with a labeled antibody purification area.
- Step (a) is a step of bringing a specimen into contact with the specimen purification area.
- samples of the “specimen” include blood (serum / plasma), urine, nasal fluid, saliva, feces, body cavity fluid (spinal fluid, ascites, pleural effusion, etc.), etc. May be used. Of these samples, blood, serum, plasma, urine, nasal fluid and saliva are preferred.
- Contact means that the specimen is contained in the liquid feed circulating in the flow path, and only one surface of the sensor substrate on which the hydrophilic polymer layer is formed is immersed in the liquid feed. A mode in which the area and the specimen are brought into contact is preferable.
- the “flow path” has a rectangular tube shape or a round tube (tube) shape, and is a place where a sensor substrate and a plasmon excitation sensor (hereinafter collectively referred to as “chip”) are installed. It is preferable that the vicinity has a rectangular tube structure, and the vicinity where the drug solution is delivered preferably has a round tube (tube) shape.
- the material is a homopolymer or copolymer containing methyl methacrylate, styrene or the like as a raw material in the chip portion or the channel top plate; polyolefin such as polyethylene, etc., and silicone rubber, Teflon (registered trademark) in the drug solution delivery portion. Polymers such as polyethylene and polypropylene are used.
- the cross section of the channel of the tip portion is preferably about 100 nm to 1 mm in length and width independently.
- a substrate is formed on an integrally molded plastic product, or a separately manufactured substrate is fixed, and the metal thin film surface is fixed.
- the SAM and hydrophilic polymer layer, etc. it can be manufactured by covering with a plastic integrally molded product corresponding to the channel top plate. If necessary, the prism can be integrated into the flow path.
- the “liquid feeding” is preferably the same as the solvent or buffer in which the specimen is diluted.
- PBS phosphate buffered saline
- TBS Tris buffered saline
- HBS HEPES buffered saline
- the temperature and time for circulating the liquid supply vary depending on the type of specimen and are not particularly limited, but are usually 20 to 40 ° C. ⁇ 1 to 60 minutes, preferably 37 ° C. ⁇ 5 to 15 minutes.
- the initial concentration of the analyte contained in the sample being fed may be 100 ⁇ g / mL to 0.001 pg / mL.
- the total amount of the liquid delivery that is, the volume of the flow path is usually 0.001 to 20 mL, preferably 0.1 to 1 mL.
- the liquid flow rate is usually 1 to 2,000 ⁇ L / min, preferably 5 to 500 ⁇ L / min.
- the step (b) is a step of bringing the sample obtained through the step (a) into contact with the sensor area.
- the specimen obtained through the above step (a) has various impurities adsorbed and adhered nonspecifically to the specimen purification area (that is, the hydrophilic polymer layer of the sensor substrate, the SAM, or the metal thin film surface).
- the analyte concentration is high.
- Contact means that such a specimen is included in the liquid supply circulating in the flow path, and only one surface on which the first ligand of the plasmon excitation sensor is immobilized is immersed in the liquid supply. A mode in which the sensor area and the specimen are brought into contact with each other is preferable.
- the cleaning step is a step of cleaning the surface of the plasmon excitation sensor obtained through the step (b) and / or the surface of the plasmon excitation sensor obtained through the following step (c).
- washing solution used in the washing step for example, a surfactant such as Tween 20, Triton X100 is dissolved in the same solvent or buffer solution used in the reaction of steps (b) and (c), Those containing 00001 to 1% by weight or 10 to 500 mM of a salt such as sodium chloride or potassium chloride are desirable.
- a low pH buffer solution for example, 10 mM Glycine HCl having a pH of 1.5 to 4.0 may be used.
- the temperature and flow rate at which the cleaning liquid is circulated are preferably the same as the temperature and flow rate at which the liquid feed in step (b) is circulated.
- the time for circulating the cleaning liquid is usually 0.5 to 180 minutes, preferably 5 to 60 minutes.
- fluorescent dye is a general term for substances that emit fluorescence by irradiating predetermined excitation light in the present invention, or excited by using an electric field effect. Including luminescence.
- the fluorescent dye used in the present invention is not particularly limited as long as it is not quenched due to light absorption by the metal thin film, and may be any known fluorescent dye.
- fluorescent dyes with large Stokes shifts that allow the use of a fluorometer with a filter rather than a monochromator and also increase the efficiency of detection are preferred.
- fluorescent dyes examples include fluorescein family fluorescent dyes (Integrated DNA Technologies), polyhalofluorescein family fluorescent dyes (Applied Biosystems Japan Co., Ltd.), and hexachlorofluorescein family fluorescent dyes. (Applied Biosystems Japan), Coumarin family fluorescent dye (Invitrogen), Rhodamine family fluorescent dye (GE Healthcare Biosciences), cyanine family fluorescent dye, Indocarbocyanine family fluorescent dye, oxazine family fluorescent dye, thiazine family fluorescent dye, squaraine family fluorescent dye, chelated lanthanide dye Millie's fluorescent dye, BODIPY® family fluorescent dye (manufactured by Invitrogen), naphthalenesulfonic acid family fluorescent dye, pyrene family fluorescent dye, triphenylmethane family fluorescent dye, Alexa Fluor (Registered trademark) dye series (manufactured by Invitrogen Corp.) and the like, and further, U.S. Patent Nos. 6,406,297, 6,22
- Table 1 shows the absorption wavelength (nm) and emission wavelength (nm) of typical fluorescent dyes included in these families.
- the fluorescent dye is not limited to the organic fluorescent dye.
- rare earth complex fluorescent dyes such as Eu and Tb can also be used as fluorescent dyes in the present invention.
- Rare earth complexes are generally characterized by a large wavelength difference between the excitation wavelength (about 310 to 340 nm) and the emission wavelength (about 615 nm for Eu complex and 545 nm for Tb complex) and a long fluorescence lifetime of several hundred microseconds or more. is there.
- An example of a commercially available rare earth complex-based fluorescent dye is ATBTA-Eu 3+ .
- a fluorescent dye having a maximum fluorescence wavelength in a wavelength region where light absorption by the metal contained in the metal thin film is small when performing fluorescence measurement described later For example, when gold is used as the metal thin film, it is desirable to use a fluorescent dye having a maximum fluorescence wavelength of 600 nm or more in order to minimize the influence of light absorption by the gold thin film. Therefore, in this case, it is particularly desirable to use a fluorescent dye having a maximum fluorescence wavelength in the near infrared region, such as Cy5, Alexa Fluor (registered trademark) 647.
- a fluorescent dye having the maximum fluorescence wavelength in the near-infrared region can minimize the influence of light absorption by iron derived from blood cell components in the blood. Is also useful.
- a fluorescent dye having a maximum fluorescence wavelength of 400 nm or more it is desirable to use.
- These fluorescent dyes may be used alone or in combination of two or more.
- conjugate of second ligand and fluorescent dye “A conjugate of a ligand (second ligand) that may be the same as or different from the ligand (first ligand) contained in the sensor area used in the present invention and a fluorescent dye”
- a secondary antibody an antibody that can recognize and bind to an analyte (target antigen) contained in the specimen is preferable.
- the second ligand is a ligand used for the purpose of labeling the analyte with a fluorescent dye, and may be the same as or different from the first ligand.
- the primary antibody used as the first ligand is a polyclonal antibody
- the secondary antibody used as the second ligand may be a monoclonal antibody or a polyclonal antibody, but the primary antibody is monoclonal.
- the secondary antibody is preferably a monoclonal antibody that recognizes an epitope that the primary antibody does not recognize, or a polyclonal antibody.
- a complex in which a second analyte that competes with an analyte (target antigen) contained in a specimen (competitive antigen; however, different from the target antigen) and a secondary antibody are bound in advance.
- the embodiment to be used is also preferable. Such an embodiment is preferable because the amount of fluorescent signal (fluorescent signal) and the amount of target antigen can be proportional.
- a carboxyl group is added to the fluorescent dye, and the carboxyl group is converted into a water-soluble carbodiimide [ WSC] (for example, 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride [EDC] and the like) and N-hydroxysuccinimide [NHS], and then an active esterified carboxyl group
- WSC water-soluble carbodiimide
- EDC 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride [EDC] and the like
- NHS N-hydroxysuccinimide
- Method of dehydrating and immobilizing an amino group possessed by a secondary antibody using water-soluble carbodiimide Method of immobilizing by reacting a secondary antibody and a fluorescent dye each having an isothiocyanate and amino group; Sulfonyl halide and amino group Reacting and fixing secondary antibody and fluorescent dye each having A method of reacting and immobil
- the concentration of the conjugate of the second ligand and fluorescent dye produced in this manner during feeding is preferably 0.001 to 10,000 ⁇ g / mL, more preferably 1 to 1,000 ⁇ g / mL.
- the temperature, time, and flow rate at which the liquid is circulated are the same as in the above step (b).
- Step (x) is a step of bringing the conjugate into contact with a labeled antibody purification area in advance. That is, the step (x) is a step of removing the labeled contaminants contained in the labeled antibody (60) by bringing the labeled antibody solution (60) into contact with the labeled antibody purification area (70) as shown in FIG. It is.
- Step (d) means that the prism is applied to the plasmon excitation sensor obtained through the step (c), preferably the washing step, from the other surface of the transparent support on which the metal thin film is not formed. In this step, the amount of fluorescence emitted from the excited fluorescent dye is measured by the SPFS detection unit.
- the light source used in the assay method of the present invention is not particularly limited as long as it can cause plasmon excitation in a metal thin film, but in terms of unity of wavelength distribution and intensity of light energy, laser light is used. Is preferably used as the light source. It is desirable to adjust the energy and photon amount immediately before the laser light enters the prism through the optical filter.
- the surface plasmon is generated on the surface of the metal thin film by the laser light irradiation under the total reflection attenuation condition [ATR]. Due to the electric field enhancement effect of surface plasmons, the fluorescent dye is excited by photons that are increased by several tens to several hundred times the amount of photons irradiated. Note that the amount of photon increase due to the electric field enhancement effect depends on the refractive index of the transparent support, the metal species of the metal thin film, and the film thickness thereof, but is usually about 10 to 20 times that of gold.
- the fluorescent dye In the fluorescent dye, electrons in the molecule are excited by light absorption, move to the first electron excited state in a short time, and when returning from this state (level) to the ground state, the wavelength of the wavelength corresponding to the energy difference Fluoresce.
- laser light for example, an LD having a wavelength of 200 to 900 nm, an 0.001 to 1,000 mW; a wavelength of 230 to 800 nm (resonance wavelength is determined by the metal species used in the metal thin film), and a semiconductor of 0.01 to 100 mW A laser etc. are mentioned.
- the “prism” is intended to allow the laser light through various filters to efficiently enter the plasmon excitation sensor, and preferably has the same refractive index as that of the transparent support.
- various prisms for which total reflection conditions can be set can be selected as appropriate, and therefore, there is no particular limitation on the angle and shape. Examples of such commercially available prisms include those similar to the above-mentioned commercially available “glass-made transparent support”.
- optical filter examples include a neutral density [ND] filter, a polarizing filter, a cut filter, a condensing lens, and a diaphragm lens.
- the “darkening [ND] filter” (or neutral density filter) is intended to adjust the amount of incident laser light. In particular, when a detector with a narrow dynamic range is used, it is preferable to use it for carrying out a highly accurate measurement.
- the “polarizing filter” is used to convert the laser light into P-polarized light that efficiently generates surface plasmons.
- Cut filters are: external light (illumination light outside the device), excitation light (excitation light transmission component), stray light (excitation light scattering component in various places), plasmon scattering light (excitation light originated from plasmon A filter that removes optical noise (such as scattered light generated by the influence of structures or deposits on the surface of the excitation sensor) and autofluorescence of the fluorescent dye, such as an interference filter and a color filter.
- the “condensing lens” is intended to efficiently collect the fluorescent signal on the detector, and may be an arbitrary condensing system.
- a simple condensing system a commercially available objective lens (for example, manufactured by Nikon Corporation or Olympus Corporation) used in a microscope or the like may be used.
- the magnification of the objective lens is preferably 10 to 100 times.
- the “SPFS detector” is preferably a photomultiplier tube [PMT] (a photomultiplier manufactured by Hamamatsu Photonics Co., Ltd.) from the viewpoint of ultra-high sensitivity. Also, although the sensitivity is lower than these, a CCD image sensor capable of multipoint measurement is also suitable because it can be viewed as an image and noise light can be easily removed.
- PMT photomultiplier tube
- CCD image sensor capable of multipoint measurement is also suitable because it can be viewed as an image and noise light can be easily removed.
- Step (e) is a step of calculating the amount of analyte contained in the specimen from the measurement result obtained in step (d).
- a calibration curve is created by performing measurement with a target antigen or target antibody at a known concentration, and an analyte in the sample to be measured (based on the created calibration curve) This is a step of calculating the amount of target antigen or target antibody) from the measurement signal.
- Nucleic acids DNA that may be single-stranded or double-stranded, RNA, polynucleotides, oligonucleotides, PNA (peptide nucleic acids) etc., or nucleosides, nucleotides and their modified molecules), proteins (polypeptides, Oligopeptides, etc.), amino acids (including modified amino acids), carbohydrates (oligosaccharides, polysaccharides, sugar chains, etc.), lipids, or their modified molecules, complexes, etc., and specifically, AFP [ It may be a carcinoembryonic antigen such as [alpha-fetoprotein], a tumor marker, a signal transmitter, a hormone, etc., and is not particularly limited.
- the obtained support was immersed in an ethanol solution containing 1 mM 11-amino-1-undecanethiol for 24 hours or more to form a SAM on one side of the gold thin film.
- the support was removed from the solution, washed with ethanol and isopropanol, and then dried with an air gun.
- step (a) 0.8 mL of commercially available bovine plasma (manufactured by Kojin Bio Co., Ltd.) to which 0.1 ng / ml AFP has been added is brought into contact with the sample purification area for 25 minutes, and the CMD adsorbing component in this bovine plasma Removed.
- step (b) 0.8 mL of bovine plasma (specimen) obtained through step (a) was brought into contact with the sensor area formed on the surface of the plasmon excitation sensor (Production Example 1) for 25 minutes.
- the sensor area was washed three times with 0.8 mL of a TBS solution containing 0.05% by weight of Tween20.
- step (c) 0.8 mL of a secondary antibody (1% BSA / PBS solution prepared to 1 ⁇ g / mL) labeled with Alexa Fluor (registered trademark) 647 is added to the sensor area and reacted for 20 minutes. I let you.
- a secondary antibody 1% BSA / PBS solution prepared to 1 ⁇ g / mL labeled with Alexa Fluor (registered trademark) 647 is added to the sensor area and reacted for 20 minutes. I let you.
- step (d) laser light (633 nm, 633 nm, from the other surface on which the plasmon excitation sensor is not formed with the gold thin film of the glass transparent support via a prism (manufactured by Sigma Kogyo Co., Ltd.) 10 ⁇ W), the signal value when the amount of fluorescence emitted from the excited fluorescent dye was measured with a photomultiplier tube [PMT] was measured and used as an “assay measurement signal”.
- the solution from which the CMD adsorbing component in bovine plasma was removed that is, the SPFS measurement signal when AFP was 0 ng / mL was defined as “blank signal”.
- step (e) the assay signal was evaluated by the following formula.
- step (x) 0.8 mL of secondary antibody labeled with Alexa fluor (registered trademark) 647 (1% BSA / PBS solution prepared to be 1,000 ng / mL) is added to the purification area for labeled antibody For 20 minutes.
- Alexa fluor registered trademark
- the reaction solution was recovered and used as a purified labeled antibody solution.
- the assay method using the combination of the purification area and the sensor area of the present invention is a method that can be detected with high sensitivity and high accuracy, for example, even a trace amount of tumor marker contained in blood can be detected. From this result, the presence of a preclinical non-invasive cancer (carcinoma in situ) that cannot be detected by palpation or the like can also be predicted with high accuracy.
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Abstract
Description
工程(b):該工程(a)を経て得られた検体を、上記センサエリアに接触させる工程,
工程(c):上記プラズモン励起センサに含まれるリガンドとは同じであっても異なっていてもよいリガンドと蛍光色素とのコンジュゲートを、該工程(b)を経たセンサエリアと反応させる工程,
工程(d):該工程(c)を経て得られたプラズモン励起センサに、上記透明支持体の、上記金属薄膜を形成していないもう一方の表面から、プリズムを経由してレーザ光を照射し、励起された蛍光色素から発光された蛍光量を測定する工程,および
工程(e):該工程(d)で得られた測定結果から、検体中に含有されるアナライト量を算出する工程。
本発明のSPFS用センサチップは、表面プラズモン励起増強蛍光分光〔SPFS〕測定用流路において、フロー方向に対して上流に精製エリアを配置し、その下流にセンサエリアを配置することを特徴とする。すなわち、SPFS用センサチップは、フロー方向に対して順に精製エリアとセンサエリアとを直列に配置したものである。
本発明に用いられるセンサエリアは、透明支持体と;該支持体の一方の表面に形成された金属薄膜と;該金属薄膜の、該支持体とは接していないもう一方の表面に形成された自己組織化単分子膜〔SAM〕と;該SAMの、該金属薄膜とは接していないもう一方の表面に形成された親水性高分子層と;該親水性高分子層の、該SAMとは接していないもう一方の表面に固定化された生理活性物質と;を含んでなるプラズモン励起センサの表面上に形成されていることが好ましく、図1に示すように、精製された検体(40)、すなわち、濃縮されたアナライトを捕捉し、SPFS測定に供されるエリアである。
本発明で用いられる「透明支持体」としては、石英製やガラス製であっても、ポリカーボネート〔PC〕,シクロオレフィンポリマー〔COP〕などのプラスチック製であってもよく、屈折率〔nd〕が好ましくは1.40~2.20であり、厚さが好ましくは0.01~10mm、より好ましくは0.5~5mmであれば、大きさ(縦×横)は特に限定されない。
上記透明支持体の一方の表面に形成された「金属薄膜」としては、好ましくは、金、銀、アルミニウム、銅、および白金からなる群から選ばれる少なくとも1種の金属からなり、より好ましくは金からなることが望ましく、これら金属の合金であってもよい。このような金属種は、酸化に対して安定であり、かつ表面プラズモンによる電場増強が大きくなることから好適である。
SAM〔Self-Assembled Monolayer;自己組織化単分子膜〕は、金属薄膜の透明支持体とは反対側の面に形成され、生理活性物質、好ましくは親水性高分子を金属薄膜に固定する際の土台としての役割を有する。
親水性高分子層は、SAMと生理活性物質との結合を介在するように設けることが好ましい。親水性高分子層を構成する親水性高分子は、多糖類としてカルボキシメチルデキストラン〔CMD〕等のデキストランが、合成樹脂としてポリアクリル酸が好ましい。
生理活性物質は、捕捉対象物質と特異的に結合するタンパク質であってもよく、例えば、抗原と特異的に結合する抗体のように、基質・補酵素に対する酵素,ホルモンに対するレセプタ,抗体に対するプロテインA・プロテインG,ビオチンに対するアビジン類,カルシウムに対するカルモジュリンなどが挙げられ、これらのうち、「捕捉対象物質と特異的に結合するタンパク質」としては抗体が好ましい。
精製エリアは、検体用精製エリアを含むことが好ましく、検体用精製エリアおよび標識抗体用精製エリアの両方を含むことがより好ましい。
検体用精製エリアは、検体に含まれるアナライト以外の非特異吸着物質、所謂夾雑物を非特異的に吸着・付着させるエリアであって、この精製エリアを通過した検体は夾雑物の一部(好ましくは全部)が除去されている。
標識抗体用精製エリアは、例えば抗体などのリガンドや微量に含まれると考えられる種々の夾雑物(非特異吸着物質・抗体であってもアナライトを認識しない抗体など)に蛍光色素を標識したコンジュゲートの混合物、すなわちリガンドと蛍光色素とのコンジュゲートと夾雑物と蛍光色素とのコンジュゲートとの混合物のうち、夾雑物と蛍光色素とのコンジュゲートを非特異的に吸着・付着させるエリアであって、この精製エリアを通過した該混合物は、主に標識抗体用精製エリアに吸着・付着した夾雑物と蛍光色素とのコンジュゲートの一部(好ましくは全部)が取り除かれている。
本発明のアッセイ法は、少なくとも、下記工程(a)~(e)を含むことを特徴とし、洗浄工程、さらに下記工程(x)を含むことが好ましい。
工程(b):該工程(a)を経て得られた検体を、センサエリアに接触させる工程,
工程(c):プラズモン励起センサに含まれるリガンドとは同じであっても異なっていてもよいリガンドと蛍光色素とのコンジュゲートを、該工程(b)を経たセンサエリアと反応させる工程,
工程(d):該工程(c)を経て得られたプラズモン励起センサに、上記透明支持体の、上記金属薄膜を形成していないもう一方の表面から、プリズムを経由してレーザ光を照射し、励起された蛍光色素から発光された蛍光量を測定する工程,および
工程(e):該工程(d)で得られた測定結果から、検体中に含有されるアナライト量を算出する工程。
工程(x):予め、上記コンジュゲートを、標識抗体用精製エリアに接触させる工程。
工程(a)とは、検体を、検体用精製エリアに接触させる工程である。
「検体」としては、例えば、血液(血清・血漿),尿,鼻孔液,唾液,便,体腔液(髄液,腹水,胸水等)などが挙げられ、所望の溶媒、緩衝液等に適宜希釈して用いてもよい。これら検体のうち、血液,血清,血漿,尿,鼻孔液および唾液が好ましい。
「接触」は、流路中に循環する送液に検体が含まれ、センサ基板の親水性高分子層が形成されている片面のみが該送液中に浸漬されている状態において、検体用精製エリアと検体とを接触させる態様が好ましい。
工程(b)とは、上記工程(a)を経て得られた検体を、センサエリアに接触させる工程である。上記工程(a)を経て得られた検体は、種々の夾雑物が検体用精製エリア(すなわち、センサ基板の親水性高分子層やSAM,金属薄膜表面)に非特異的に吸着・付着したため、そのアナライト濃度が高くなっている。
「接触」は、流路中に循環する送液にこのような検体が含まれ、プラズモン励起センサの第1のリガンドが固定化されている片面のみが該送液中に浸漬されている状態において、センサエリアと該検体とを接触させる態様が好ましい。
洗浄工程とは、上記工程(b)を経て得られたプラズモン励起センサの表面および/または下記工程(c)を経て得られたプラズモン励起センサの表面を洗浄する工程である。
工程(c):プラズモン励起センサに含まれるリガンド(第1のリガンド)とは同じであっても異なっていてもよいリガンド(第2のリガンド)と蛍光色素とのコンジュゲートを、上記工程(b)、好ましくは上記洗浄工程を経たセンサエリアと反応させる工程である。
「蛍光色素」とは、本発明において、所定の励起光を照射する、または電界効果を利用して励起することによって蛍光を発光する物質の総称であり、該「蛍光」は、燐光など各種の発光も含む。
「本発明で用いられるセンサエリアに含まれるリガンド(第1のリガンド)とは同じであっても異なっていてもよいリガンド(第2のリガンド)と蛍光色素とのコンジュゲート」は、リガンドとして2次抗体を用いる場合、検体中に含有されるアナライト(標的抗原)を認識し結合し得る抗体であることが好ましい。
工程(x)とは、予め、上記コンジュゲートを、標識抗体用精製エリアに接触させる工程である。すなわち、工程(x)とは、図2に示すように、標識抗体用精製エリア(70)に標識抗体液(60)を接触させることで標識抗体(60)に含まれる標識夾雑物を取り除く工程である。
工程(d)とは、上記工程(c)、好ましくは上記洗浄工程を経て得られたプラズモン励起センサに、上記透明支持体の、上記金属薄膜を形成していないもう一方の表面から、プリズムを経由してレーザ光を照射し、励起された蛍光色素から発光された蛍光量をSPFS検出部により測定する工程である。
本発明のアッセイ法で用いる光源は、金属薄膜にプラズモン励起を生じさせることができるものであれば、特に制限がないものの、波長分布の単一性および光エネルギーの強さの点で、レーザ光を光源として用いることが好ましい。レーザ光は、光学フィルタを通して、プリズムに入射する直前のエネルギーおよびフォトン量を調節することが望ましい。
工程(e)とは、上記工程(d)で得られた測定結果から、検体中に含有されるアナライト量を算出する工程である。
「アナライト」としては、第1のリガンドに特異的に認識され(または、認識し)結合し得る分子または分子断片であって、このような「分子」または「分子断片」としては、例えば、核酸(一本鎖であっても二本鎖であってもよいDNA,RNA,ポリヌクレオチド,オリゴヌクレオチド,PNA(ペプチド核酸)等,またはヌクレオシド,ヌクレオチドおよびそれらの修飾分子),タンパク質(ポリペプチド,オリゴペプチド等),アミノ酸(修飾アミノ酸も含む。),糖質(オリゴ糖,多糖類,糖鎖等),脂質,またはこれらの修飾分子,複合体などが挙げられ、具体的には、AFP〔αフェトプロテイン〕などのがん胎児性抗原や腫瘍マーカー,シグナル伝達物質,ホルモンなどであってもよく、特に限定されない。
さらに、工程(e)は、上記工程(b)の前に測定したシグナルを"ブランクシグナル"、としたとき、下記式で表されるアッセイシグナル変化量を算出することができる。
まず、屈折率〔nd〕1.72,厚さ1mmのガラス製の透明支持体((株)オハラ製の「S-LAL 10」)をプラズマ洗浄し、該支持体の片面にクロム薄膜をスパッタリング法により形成した後、その表面にさらに金薄膜をスパッタリング法により形成した。クロム薄膜の厚さは1~3nm,金薄膜の厚さは44~52nmであった。
抗αフェトプロテイン〔AFP〕モノクローナル抗体(1D5)を含まないMESを用いた以外は作製例1と同様にして、支持体表面にCMDが固相化されたセンサ基板を作製した。
はじめに、センサ基板(作製例2)の表面上に形成された検体用精製エリアを、Tween20を0.05重量%含むTBS溶液0.8mLで3回洗浄した。
得られた結果を図5に示す。
はじめに、プラズモン励起センサ(作製例1)の表面上に形成された標識抗体用精製エリアを、Tween20を0.05重量%含むTBS溶液0.8mLで3回洗浄した。
未精製のウシ血漿を用いた以外は実施例1と同様にしてアッセイ法を実施した。得られた結果を図5に示す。
検体として、0.1ng/mlのAFPを添加した1重量%BSAを含むPBS溶液を用いた以外は実施例1と同様にしてアッセイ法を実施した。得られた結果を図6に示す。
20・・・・・・検体
21・・・・・・アナライト
22,23・・・非特異吸着物質
30・・・・・・検体用精製エリア
31・・・・・・その一方の表面に金属薄膜が形成された透明支持体
32・・・・・・SAM
33・・・・・・カルボキシメチルデキストラン〔CMD〕
40・・・・・・精製された検体
50・・・・・・センサエリア
51・・・・・・その一方の表面に金属薄膜が形成された透明支持体
52・・・・・・SAM
53・・・・・・カルボキシメチルデキストラン〔CMD〕
54・・・・・・抗体(生理活性物質)
60・・・・・・標識抗体液
61・・・・・・標識抗体
70・・・・・・標識抗体用精製エリア
71・・・・・・その一方の表面に金属薄膜が形成された透明支持体
72・・・・・・SAM
73・・・・・・カルボキシメチルデキストラン〔CMD〕
74・・・・・・抗体
A・・・・・・バッファーより少し高め
Claims (11)
- 表面プラズモン励起増強蛍光分光〔SPFS〕測定用流路において、
フロー方向に対して上流に精製エリアを配置し、
その下流にセンサエリアを配置することを特徴とするSPFS用センサチップ。 - 上記センサエリアが、
透明支持体と;
該支持体の一方の表面に形成された金属薄膜と;
該金属薄膜の、該支持体とは接していないもう一方の表面に形成された自己組織化単分子膜〔SAM〕と;
該SAMの、該金属薄膜とは接していないもう一方の表面に形成された親水性高分子層と;
該親水性高分子層の、該SAMとは接していないもう一方の表面に固定化された生理活性物質と;
を含んでなるプラズモン励起センサの表面上に形成されている請求項1に記載のSPFS用センサチップ。 - 上記精製エリアが、検体用精製エリアを含む請求項1または2に記載のSPFS用センサチップ。
- 上記精製エリアが、検体用精製エリアおよび標識抗体用精製エリアを含む請求項1または2に記載のSPFS用センサチップ。
- 上記検体用精製エリアが、
透明支持体と;
該支持体の一方の表面に形成された金属薄膜と;
該金属薄膜の、該支持体とは接していないもう一方の表面に形成された自己組織化単分子膜〔SAM〕と;
該SAMの、該金属薄膜とは接していないもう一方の表面に形成された親水性高分子層と;
を含んでなるセンサ基板の表面上に形成されている請求項3または4に記載のSPFS用センサチップ。 - 上記標識抗体用精製エリアが、
透明支持体と;
該支持体の一方の表面に形成された金属薄膜と;
該金属薄膜の、該支持体とは接していないもう一方の表面に形成されたSAMと;
該SAMの、該金属薄膜とは接していないもう一方の表面に形成された親水性高分子層と;
該親水性高分子層の、該SAMとは接していないもう一方の表面に固定化された生理活性物質と;
を含んでなるプラズモン励起センサの表面上に形成されている請求項4に記載のSPFS用センサチップ。 - 上記精製エリアが、検体用精製エリアおよび標識抗体用精製エリアを含み、
上記標識抗体用精製エリアが、上記プラズモン励起センサの表面上に形成されている請求項2に記載のSPFS用センサチップ。 - 透明支持体と;
該支持体の一方の表面に形成された金属薄膜と;
該金属薄膜の、該支持体とは接していないもう一方の表面に形成されたSAMと;
該SAMの、該金属薄膜とは接していないもう一方の表面に形成された親水性高分子層と;
を含んでなるセンサ基板、および
透明支持体と;
該支持体の一方の表面に形成された金属薄膜と;
該金属薄膜の、該支持体とは接していないもう一方の表面に形成されたSAMと;
該SAMの、該金属薄膜とは接していないもう一方の表面に形成された親水性高分子層と;
該親水性高分子層の、該SAMとは接していないもう一方の表面に固定化された生理活性物質と;
を含んでなるプラズモン励起センサを備え、
該センサ基板に含まれる親水性高分子層を構成する親水性高分子と、
該プラズモン励起センサに含まれる親水性高分子層を構成する親水性高分子とが
異なっている請求項1~7のいずれか一項に記載のSPFS用センサチップ。 - 上記精製エリアが、上記センサエリアから上流の流路すべてに配置されている請求項1~8のいずれか一項に記載のSPFS用センサチップ。
- 少なくとも、下記工程(a)~(e)を含むことを特徴とするアッセイ法;
工程(a):検体を、請求項3~6のいずれか一項に記載の検体用精製エリアに接触させる工程,
工程(b):該工程(a)を経て得られた検体を、請求項1~8のいずれか一項に記載のセンサエリアに接触させる工程,
工程(c):請求項2および6~8のいずれか一項に記載のプラズモン励起センサに含まれるリガンドとは同じであっても異なっていてもよいリガンドと蛍光色素とのコンジュゲートを、該工程(b)を経たセンサエリアと反応させる工程,
工程(d):該工程(c)を経て得られたプラズモン励起センサに、上記透明支持体の、上記金属薄膜を形成していないもう一方の表面から、プリズムを経由してレーザ光を照射し、励起された蛍光色素から発光された蛍光量を測定する工程,ならびに
工程(e):該工程(d)で得られた測定結果から、検体中に含有されるアナライト量を算出する工程。 - さらに、下記工程(x)を含む請求項10に記載のアッセイ法;
工程(x):予め、上記コンジュゲートを、請求項4~7のいずれかに記載の標識抗体用精製エリアに接触させる工程。
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JP2012529537A JP5821852B2 (ja) | 2010-08-17 | 2011-07-27 | 非特異吸着の精製機構を備えたspfs用センサ |
US13/817,356 US9134235B2 (en) | 2010-08-17 | 2011-07-27 | SPFS sensor equipped with mechanism purifying non-specifically adsorptive contaminants |
EP11818034.8A EP2607888B1 (en) | 2010-08-17 | 2011-07-27 | Spfs sensor equipped with non-specific adsorption type purification mechanism |
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US (1) | US9134235B2 (ja) |
EP (1) | EP2607888B1 (ja) |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2014087802A1 (ja) | 2012-12-05 | 2014-06-12 | コニカミノルタ株式会社 | 表面プラズモン励起増強蛍光分光法(spfs)を用いた免疫測定法における夾雑物由来の非特異的シグナルの抑制方法 |
WO2015129361A1 (ja) * | 2014-02-26 | 2015-09-03 | コニカミノルタ株式会社 | 表面プラズモン励起増強蛍光分光測定用センサーチップ |
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EP3273226A4 (en) * | 2015-03-17 | 2018-01-31 | Konica Minolta, Inc. | Detection device |
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EP2607888A4 (en) | 2015-08-19 |
US20130143332A1 (en) | 2013-06-06 |
EP2607888A1 (en) | 2013-06-26 |
JP5821852B2 (ja) | 2015-11-24 |
US9134235B2 (en) | 2015-09-15 |
EP2607888B1 (en) | 2022-01-12 |
JPWO2012023391A1 (ja) | 2013-10-28 |
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