WO2011134411A1 - 大豆浓缩蛋白的生产方法 - Google Patents

大豆浓缩蛋白的生产方法 Download PDF

Info

Publication number
WO2011134411A1
WO2011134411A1 PCT/CN2011/073462 CN2011073462W WO2011134411A1 WO 2011134411 A1 WO2011134411 A1 WO 2011134411A1 CN 2011073462 W CN2011073462 W CN 2011073462W WO 2011134411 A1 WO2011134411 A1 WO 2011134411A1
Authority
WO
WIPO (PCT)
Prior art keywords
soybean
extraction
hexane
protein concentrate
soybean meal
Prior art date
Application number
PCT/CN2011/073462
Other languages
English (en)
French (fr)
Inventor
祁鲲
Original Assignee
Qi Kun
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qi Kun filed Critical Qi Kun
Publication of WO2011134411A1 publication Critical patent/WO2011134411A1/zh

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
    • A23J1/142—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds by extracting with organic solvents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/14—Vegetable proteins
    • A23J3/16—Vegetable proteins from soybean
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a method for producing soy protein concentrate. Specifically, the method uses the hexane to extract the soybean oil, and then separates the extracted solid phase and the liquid phase, and directly separates the solid phase (soybean meal containing partial hexane (hereinafter, referred to as "wet soybean meal”). ”)) As a raw material, an aqueous solution of ethanol is used as an extractant for extraction, and then the solid phase and the liquid phase after extraction are separated, and the soybean protein is prepared by further treating the solid phase.
  • Wet soybean meal wet soybean meal
  • soy protein concentrate is processed by pulverization, peeling, leaching oil, separation, ethanol extraction, drying, etc., and removes oil and low molecular soluble non-protein components in soybean (mainly It is a soybean deep-processed product obtained after being soluble sugar, ash, alcohol-soluble protein and various odor substances.
  • the method for producing soy protein concentrate at home and abroad mainly uses dry soybean soybean meal as raw material and ethanol as extracting agent to extract the sugar substance in soybean meal, thereby increasing the protein content in soybean meal and meeting the requirements of concentrated protein products. Protein content.
  • the dried soybean meal is prepared as follows: using soybean as a raw material, after cleaning, peeling, and rolling, the oil in the soybean flake is extracted by using hexane, and after fully extracting, the oil in the soybean flake is extracted. About 95%.
  • the solid phase and the liquid phase after the extraction are separated, and the obtained solid phase (i.e., the soybean flakes which have not evaporated the hexane solvent) is wet soybean meal (generally, wet soybean meal contains 25-35 wt% of hexamidine).
  • the wet soybean meal can be subjected to a process such as solvent removal, drying, packaging, etc. to obtain a qualified, dry soybean meal.
  • This dry soybean meal has a high powder content, coupled with packaging, transportation and other processes, and the cost of raw materials is higher, which brings certain difficulties to the use of this soybean meal to produce soy protein concentrate. Summary of the invention
  • the present invention proposes a method for directly producing soybean protein concentrate by using wet soybean meal as a raw material.
  • the method of the present invention can shorten the process flow and greatly reduce the cost of raw materials.
  • the method of the invention extracts the wet soybean meal by using an aqueous solution of ethanol as an extracting agent, and then separates the solid phase and the liquid phase after extraction, and further processes the solid phase to prepare a soybean protein concentrate, and further processes the liquid phase.
  • a method for producing soy protein concentrate includes the following steps Step 1: Step 1.
  • soybean as a raw material, after peeling and rolling, preparing a soybean flake, extracting the soybean flakes with hexane, separating the extracted solid phase and liquid phase, and the solid phase is Wet soybean meal; Step 2. Using the wet soybean meal as a raw material, extracting the wet soybean meal directly with an aqueous solution of ethanol as an extracting agent, and then separating the solid phase and the liquid phase; Step 3. Removing the above step 2 Ethanol, hexane and water in the solid phase obtained to obtain the soy protein concentrate.
  • the ethanol, hexane and water in the liquid phase obtained in the above step 2 are removed to obtain a soy molasses by-product, which is also rich in soy isoflavones and soy saponins.
  • the obtained wet soybean meal contains 25-35% of hexanyl.
  • the concentration of the aqueous ethanol solution is 60 to 75% (v/v)
  • the weight ratio of the aqueous ethanol solution to the wet soybean meal is 6: (1 to 2)
  • the extraction temperature is 38 to 58. °C
  • extraction time is 90 ⁇ 180 minutes.
  • the extraction in the step 2 is countercurrent extraction.
  • a dry protein content obtained by using a weight ratio of aqueous ethanol to wet soybean meal of 6:1 is higher than a dry protein content of a weight ratio of 3:1.
  • the higher the dry protein content of the obtained soy protein concentrate product, for example, the extraction of 180 minutes is higher than the dry protein content obtained by 90 minutes.
  • the ruthenium in the liquid phase obtained in the step 1 can be distilled off, condensed, and recycled.
  • the ethanol, hexane and water in the liquid phase obtained in the step 2 can be distilled off, condensed, naturally separated, and recycled.
  • the process for preparing the wet soybean meal used in the present invention can be carried out by any of the prior art processes, for example, by the following method: using soybean as a raw material, after cleaning, peeling, and rolling, a soy flake is prepared, and hexane is used as an extract.
  • the solvent is used to extract the oil and fat in the soybean flakes by countercurrent extraction. After sufficient extraction, the extraction solvent contains 15-30% by weight of oil and fat in the hexane, and the extracted solid phase and liquid phase are separated.
  • the ruthenium in the obtained liquid phase is evaporated using a hexane evaporation apparatus to obtain a crude oil product.
  • the evaporated hexane solvent can be recycled after being condensed.
  • the soybean meal protein is prepared by directly using the wet soybean meal as a raw material.
  • the specific preparation steps are as follows: using wet soybean meal as a raw material, extracting the wet soybean meal directly with an aqueous solution of ethanol as an extracting agent, and then performing solid phase and liquid phase separation; then removing ethanol, hexanide and the solid phase in the obtained solid phase. Moisture, the soy protein concentrate is obtained.
  • the concentration of the aqueous ethanol solution used above may be 60% to 75% (v/v. Further, the weight ratio of the aqueous ethanol solution to the wet soybean meal is preferably 6: (1 to 2). Further, the extraction temperature is preferably 38 to 58 ° C. The extraction time is preferably from 90 to 180 minutes.
  • the extraction with an aqueous ethanol solution may be various conventional extraction methods used in solid-liquid extraction, preferably a countercurrent extraction method. Removal of the ethanol in the separated solid phase, After hydrating and hydrating, it is pulverized to obtain a powdery soy protein concentrate. The removal of ethanol, hexane and moisture can be carried out using a vertical multi-layer dryer.
  • the pulverization can be carried out using various conventional equipment and conventional The method is carried out, and there is no limitation on the particle size of the pulverization, and it can be selected as needed.
  • the direct production of soy protein concentrate using wet soybean meal simplifies the product flow and greatly reduces the product cost.
  • the liquid phase obtained by separating the solid phase and the liquid phase after extraction with an aqueous solution of ethanol ethanol and hexose are recovered, and soybean molasses is obtained.
  • the extracted molasses is also rich in alcohol-soluble substances such as soy isoflavones and soybean saponins, and water-soluble substances, which creates conditions for the extraction of soybean isoflavones and soybean saponins.
  • alcohol-soluble substances such as soy isoflavones and soybean saponins
  • water-soluble substances which creates conditions for the extraction of soybean isoflavones and soybean saponins.
  • the ethanol and hexanyl in the recovered liquid phase can be carried out using a falling film evaporator or a vacuum thin film evaporator.
  • the process for producing soy protein concentrate of the present invention can be carried out continuously under completely closed process conditions. More specific embodiments are specifically described below: Step 1.
  • soybean as a raw material, after cleaning, peeling, and rolling, a soy flake is prepared, and the oil in the soybean flake is extracted using hexane, and the extracted solid phase is separated.
  • the hexane in the obtained liquid phase was evaporated using a hexane evaporation apparatus to obtain a crude fat product.
  • the evaporated hexane is condensed and can be recycled.
  • the hexane solvent contained in the soybean flakes as the solid phase was not evaporated, and such a soybean flake containing about 25 to 35 wt% of S? was wet soybean meal.
  • the present invention is mainly characterized in that the soybean meal protein is prepared by directly using the wet soybean meal as a raw material.
  • Step 2 Using a 60% ⁇ 75% (v/v) aqueous solution of ethanol as an extractant, the wet soybean meal prepared in the first step is directly subjected to countercurrent extraction, wherein the weight ratio of the aqueous ethanol solution to the wet soybean meal is 6: (1) ⁇ 2), extraction temperature 38 ⁇ 58 ° C, extraction time 90 ⁇ 180 minutes. Thereby, water-soluble substances such as sugars and alcohol-soluble substances in the wet soybean meal are extracted.
  • the wet soybean meal is expanded by about 40% after being extracted with an aqueous solution of ethanol. The solid phase and liquid phase separation are then carried out.
  • a powdery soybean protein concentrate can be obtained.
  • the ethanol, hexane and water removed from the solid phase can be recycled after being recovered and separated.
  • Step 3 The liquid phase separated from the step 2 contains 5-15 wt% of water-soluble and alcohol-soluble substances such as sugars, and a small amount of hexane solvent is entrained, and ethanol and a small amount of hexane are evaporated by an ethanol evaporation device. When it is taken out, soy molasses having a water content of 40 to 60% by weight can be obtained.
  • the evaporated ethanol and a small amount of hexane solvent are condensed, separated by natural stratification, and the separated ethanol and hexane are recycled to the respective solvent systems for recycling.
  • the process is performed under completely closed process conditions.
  • the above two extractions can be performed in different extraction sections in the same leaching apparatus, It can be separately completed in two series of leaching equipments; the leaching equipment can adopt the commonly used flat-type leaching device, crawler type leaching device, annular leaching device and the like.
  • 100 kg of wet soybean meal was taken, placed in a leaching apparatus, and subjected to countercurrent extraction with 600 kg of a 60% (v/v) aqueous solution of ethanol, and the extraction temperature was 38 ° C, and the extraction time was 180 minutes. Thereby, water-soluble substances such as sugars and alcohol-soluble substances in the wet soybean meal are extracted.
  • the solid phase and the liquid phase are separated, and the solid phase obtained by the countercurrent extraction is subjected to removal of ethanol, hexane and water and pulverized to obtain a powdery soybean protein concentrate.
  • Protein analysis of the obtained soy protein concentrate using the internationally recognized semi-micro-Kjeldahl method showed that the obtained soy protein concentrate product had a dry protein content of 70% by weight, which met the quality standard of soybean protein concentrate in commercial trade. .
  • Example 3 Take 100 kg of the same wet soybean meal as in Example 1, placed in a leaching apparatus, and subjected to countercurrent extraction with 450 kg of a 75% (v/v) aqueous solution of ethanol, the extraction temperature was 50 ° C, and the extraction time was 90 minutes, as in Example 1. Methods and procedures for preparing soy protein concentrate.
  • the soy protein concentrate product obtained had a dry protein content of 62% by weight.
  • Example 4 Take 100 kg of the same wet soybean meal as in Example 1, placed in a leaching apparatus, and subjected to countercurrent extraction with 400 kg of a 70% (v/v) aqueous solution of ethanol, the extraction temperature was 58 ° C, and the extraction time was 150 minutes, as in Example 1. Methods and procedures for preparing soy protein concentrate.
  • the soy protein concentrate product obtained had a dry protein content of 67% by weight.
  • Example 5 Take 100 kg of the same wet soybean meal as in Example 1, placed in a leaching apparatus, and subjected to countercurrent extraction with 300 kg of a 60% (v/v) aqueous solution of ethanol, the extraction temperature was 45 ° C, and the extraction time was 160 minutes, as in Example 1. Method and stepwise preparation of soy protein concentrate.
  • the soy protein concentrate product obtained had a dry protein content of 61% by weight.
  • Example 1 Take 100 kg of the same wet soybean meal as in Example 1, placed in a leaching apparatus, and subjected to countercurrent extraction with 540 kg of a 65% (v/v) aqueous ethanol solution, the extraction temperature was 55 ° C, and the extraction time was 120 minutes, as in Example 1.
  • the soy protein concentrate product obtained had a dry protein content of 66% by weight. It can be seen that the content of the dry protein in the soy protein concentrate product produced by the method of the present invention is more than 60% by weight, which is equivalent to the protein content in the currently marketed soy protein concentrate product, and the method for producing soy protein concentrate of the present invention has The process is shortened and the advantages of raw material costs are greatly reduced.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Beans For Foods Or Fodder (AREA)
  • Peptides Or Proteins (AREA)

Description

大豆浓缩蛋白的生产方法
技术领域
本发明涉及一种大豆浓缩蛋白的生产方法。 具体来说， 所述的方法使用 己垸萃取大豆油脂后， 分离萃取后的固相和液相， 直接以分离出的固相 （含 有部分己烷的大豆豆粕 （下文中， 称为 "湿豆粕" ）） 为原料， 采用乙醇水溶 液为萃取剂进行萃取， 然后将萃取后的固相和液相分离， 通过对固相进一步 处理来制得大豆浓缩蛋白。 背景技术
现有技术中， 大豆浓缩蛋白是以大豆为原料， 经过粉碎、 去皮、 浸提油 脂、 分离、 乙醇萃取、 干燥等加工工艺， 去除了大豆中的油脂、 低分子可溶 性非蛋白组分 （主要是可溶性糖、 灰分、 醇溶性蛋白和各种气味物质等） 后 所得到的大豆深加工产品。 目前国内外生产大豆浓缩蛋白的方法主要是以干燥的大豆豆粕为原料， 以乙醇为萃取剂， 将豆粕中的糖类物质萃取出来， 从而使豆粕中的蛋白含量 提高， 达到浓缩蛋白产品所要求的蛋白含量。 通常， 所述干燥的大豆豆粕按 照如下方式进行制备： 以大豆为原料， 通过清理、 脱皮、 轧片后， 使用己垸 萃取大豆片中的油脂， 经充分萃取后， 大豆片中的油脂被萃取出 95%左右。 分离萃取后的固相和液相， 所得的固相 （即， 未蒸发己垸溶剂的大豆片） 即 为湿豆粕 （一般而言， 湿豆粕中含有 25-35wt%的己垸）。 所述湿豆粕经脱除 溶剂、 烘干、 包装等工艺过程， 方能得到合格的、 干燥的大豆豆粕。 这种干 燥的大豆豆粕粉末度高， 再加上包装、 运输等过程， 原料成本更高， 给使用 这种豆粕生产大豆浓缩蛋白带来了一定的困难。 发明内容
为解决上述技术问题， 本发明提出直接使用湿豆粕为原料来制得大豆浓 缩蛋白的方法。 本发明的方法能够缩短工艺流程， 并大幅降低原料成本。 本发明的方法采用乙醇水溶液为萃取剂对所述湿豆粕进行萃取， 然后将 萃取后的固相和液相分离， 通过对固相进一步处理来制得大豆浓缩蛋白， 并 通过对液相进一步处理来制得大豆糖蜜副产品。 具体而言， 一方面， 按照本发明， 生产大豆浓缩蛋白的方法包括以下步 骤: 歩骤 1. 以大豆为原料， 经脱皮、 轧片后， 制得大豆片， 使用己烷对所述 大豆片进行萃取， 分离萃取后的固相和液相， 所述固相即为湿豆粕； 歩骤 2. 以所述湿豆粕为原料， 用乙醇水溶液为萃取剂直接对所述湿豆粕 进行萃取， 之后进行固相和液相的分离； 歩骤 3. 脱除上述歩骤 2中所得固相中的乙醇、 己烷和水分， 得到所述大 豆浓缩蛋白。 另一方面， 脱除上述歩骤 2 中所得液相中的乙醇、 己烷和水分， 得到大 豆糖蜜副产品， 此副产品中还富含大豆异黄酮和大豆皂甙。 所述歩骤 1中， 所得湿豆粕中含有 25-35^%的己垸。 所述步骤 2 中， 所述乙醇水溶液的浓度为 60〜75% ( v/v) , 所述乙醇水 溶液与所述湿豆粕的重量比为 6:(1〜2)， 萃取温度为 38〜58 °C， 萃取时间为 90〜180分钟。 优选地， 所述歩骤 2中的萃取为逆流萃取。 所述步骤 2 的萃取中， 使用的乙醇水溶液的浓度越低， 所获得的大豆浓 缩蛋白产品的干基蛋白含量越高。例如， 使用乙醇水溶液的浓度为 60% ( v/v) 所获得的干基蛋白含量高于使用浓度为 75% ( v/v ) 的干基蛋白含量。 所述步骤 2 的萃取中， 对于乙醇水溶液与湿豆粕的重量比， 使用的重量 比越高， 所获得的大豆浓缩蛋白产品的干基蛋白含量越高。 例如， 使用乙醇 水溶液与湿豆粕的重量比为 6: 1所获得的干基蛋白含量高于重量比为 3: 1的干 基蛋白含量。 所述步骤 2 的萃取中， 萃取温度越高， 所获得的大豆浓缩蛋白产品的干 基蛋白含量越高， 例如， 在 58°C进行萃取所获得的干基蛋白含量高于在 38°C 进行萃取的所获得的干基蛋白含量。 所述步骤 2 的萃取中， 对于萃取时间， 萃取时间越长， 所获得的大豆浓 缩蛋白产品的干基蛋白含量越高， 例如， 萃取 180分钟比 90分钟所获得的干 基蛋白含量更高。 优选地， 可以将所述步骤 1所得液相中的己垸蒸出， 冷凝， 并循环使用。 优选地， 可以将步骤 2所得液相中的乙醇、 己烷和水分蒸出， 冷凝， 自 然分层分离， 并循环使用。 具体实施方式
下面对本发明进行具体说明。 制备本发明所用湿豆粕的工艺可以用现有技术中的任何工艺， 比如， 可 以用如下方法制备： 以大豆为原料， 通过清理、 脱皮、 轧片后， 制得大豆片， 使用己垸作为萃取溶剂， 逆流萃取大豆片中的油脂， 经充分萃取后， 萃取溶 剂己烷中含油脂 15-30wt%， 分离萃取后的固相和液相。 使用己垸蒸发设备， 将所得液相中的己垸蒸发出去， 得到粗油脂产品。 这里， 蒸发出的己烷溶剂 冷凝后可以循环使用。 经过己垸萃取后， 大豆片中的油脂被萃取出 95%左右。 此时， 作为固相的大豆片中含有的己垸溶剂未进行蒸发， 这种大约含有己垸 25-35wt%的大豆片即为湿豆粕。 本发明中， 将所述湿豆粕直接作为原料来制备大豆浓缩蛋白。 具体的制 备歩骤为： 以湿豆粕为原料， 用乙醇水溶液为萃取剂直接对所述湿豆粕进行 萃取， 之后进行固相和液相分离； 然后脱除所得固相中的乙醇、 己垸和水分， 得到所述大豆浓缩蛋白。 以上采用的乙醇水溶液的浓度可为 60%〜75% ( v/v 此外， 优选乙醇水 溶液与湿豆粕的重量比为 6:( 1〜2)。 另外， 萃取温度优选为 38〜58°C， 萃取 时间优选为 90〜180分钟。 用乙醇水溶液进行萃取的方法可以是固 -液萃取中使用的各种常规萃取方 法， 优选为逆流萃取方法。 脱除上述分离出的固相中的乙醇、 己垸和水分后， 经过粉碎即得到粉末 状的大豆浓缩蛋白。 所述脱除乙醇、 己垸和水分可以采用立式多层式的烘干机进行。 所述粉碎可以使用各种常规设备和常规方法进行， 对于粉碎的粒度没有 限制， 可以根据需要进行选择。 根据本发明的方法， 使用湿豆粕直接生产大豆浓缩蛋白， 简化了产品流 程， 大幅度降低了产品成本。 另一方面， 对于通过用乙醇水溶液萃取后， 分离固相和液相得到的液相， 回收乙醇和己垸后， 即可得到大豆糖蜜。 如此， 根据本发明的方法， 萃取得 到的糖蜜中还富含大豆异黄酮、 大豆皂甙等醇溶性物质和水溶性物质， 为大 豆异黄酮和大豆皂甙的提取创造了条件。 所述回收液相中的乙醇和己垸可以采用降膜式蒸发器或真空薄膜蒸发器 进行。 本发明的生产大豆浓缩蛋白的方法可以在完全封闭的操作工艺条件下连 续完成。 下面具体说明更为优选的具体实施方式： 步骤 1. 以大豆为原料， 通过清理、 脱皮、 轧片后， 制得大豆片， 使用己 垸萃取大豆片中的油脂， 分离萃取后的固相和液相， 使用己垸蒸发设备， 将 所得液相中的己烷蒸发出去， 得到粗油脂产品。 这里， 蒸发出的己烷冷凝后可以循环使用。 此时， 作为固相的大豆片中 含有的己烷溶剂未进行蒸发， 这种大约含有 25-35wt% S垸的大豆片即为湿豆 粕。 本发明的特点主要在于， 将所述湿豆粕直接作为原料来制备大豆浓缩蛋 白。 歩骤 2. 用 60%〜75% ( v/v ) 的乙醇水溶液作为萃取剂， 对歩骤 1制备的 湿豆粕直接进行逆流萃取，其中， 乙醇水溶液与湿豆粕的重量比为 6: ( 1〜2)， 萃取温度 38〜58°C， 萃取时间 90〜180分钟。 由此将湿豆粕中的糖类等水溶性物质和醇溶性物质萃取出来。 湿豆粕经 乙醇水溶液萃取后体积会膨胀 40%左右。 之后进行固相和液相分离。 分离得到的固相经脱除溶剂和粉碎后， 即可得到粉末状的大豆浓缩蛋白。 从固相脱除出来的乙醇、 己烷和水分经回收分离后可以循环使用。 步骤 3. 从步骤 2分离的液相中含有 5-15wt%的糖类等水溶性和醇溶性物 质， 并夹带有少量的己烷溶剂， 通过乙醇蒸发设备将其中的乙醇和少量的己 烷蒸发出去， 可得到含水量 40-60wt%的大豆糖蜜。 优选地， 蒸发出的乙醇和少量的己垸溶剂冷凝后， 自然分层分离， 分离 出的乙醇和己烷进入各自的溶剂系统循环使用。 在本具体实施方式中， 所述操作过程均是在完全封闭的工艺条件下完成 的。 此外， 使用己垸从大豆片中萃取油脂和使用乙醇水溶液萃取湿豆粕中的 糖类等水溶性物质和醇溶性物质， 上述两次萃取可以在同一个浸出设备中的 不同萃取段中完成， 也可以在两个串联的浸出设备中分别完成； 浸出设备可 以采用目前常用的平转式浸出器、 履带式浸出器、 环形浸出器等。 实施例
下面给出具体的实施例， 对本发明生产大豆浓缩蛋白的方法进行说明， 但本发明并不限于此。 实施例 1
以大豆为原料， 经脱皮、 轧片后， 制得大豆片， 使用己烷对所述大豆片 进行萃取，分离萃取后的固相和液相，所述固相即为湿豆粕。取湿豆粕 100kg, 置于浸出设备中， 用 60% ( v/v) 的乙醇水溶液 600kg进行逆流萃取， 萃取温 度 38°C， 萃取时间 180分钟。 由此将湿豆粕中的糖类等水溶性物质和醇溶性 物质萃取出来。 萃取后将固相和液相分离， 将逆流萃取后所得的固相脱除乙醇、 己烷和 水分并经粉碎， 得到粉末状的大豆浓缩蛋白。 对所获得大豆浓缩蛋白采用国 际公认的半微量凯氏定氮法进行蛋白分析， 表明： 所获得的大豆浓缩蛋白产 品的干基蛋白含量为 70wt%， 达到了商业贸易中大豆浓缩蛋白的质量标准。 萃取所得液相， 其中含有 5- 15^%的糖类等水溶性物质和醇溶性物质并 夹带有少量的己烷溶剂， 使用乙醇蒸发设备， 将其中的乙醇和少量的己垸蒸 发， 可得到含水量 40-60wt%的糖蜜类副产物， 所得到的糖蜜副产物的性能指 标与传统工艺的性能指标相同。 实施例 2
取与实施例 1相同的湿豆粕 100kg, 置于浸出设备中， 用 75% ( v/v) 的 乙醇水溶液 450kg进行逆流萃取， 萃取温度 50°C， 萃取时间 90分钟， 按实施 例 1 中的方法和步骤制取大豆浓缩蛋白。 所获得的大豆浓缩蛋白产品的干基 蛋白含量为 62wt%。 实施例 3
取与实施例 1相同的湿豆粕 100kg, 置于浸出设备中， 用 70% ( v/v) 的 乙醇水溶液 400kg进行逆流萃取， 萃取温度 58 °C， 萃取时间 150分钟， 按实 施例 1 中的方法和步骤制取大豆浓缩蛋白。 所获得的大豆浓缩蛋白产品的干 基蛋白含量为 67wt%。 实施例 4
取与实施例 1相同的湿豆粕 100kg, 置于浸出设备中， 用 60% ( v/v) 的 乙醇水溶液 300kg进行逆流萃取， 萃取温度 45 °C， 萃取时间 160分钟， 按实 施例 1 中的方法和歩骤制取大豆浓缩蛋白。 所获得的大豆浓缩蛋白产品的干 基蛋白含量为 61wt%。 实施例 5
取与实施例 1相同的湿豆粕 100kg, 置于浸出设备中， 用 65% ( v/v) 的 乙醇水溶液 540kg进行逆流萃取， 萃取温度 55 °C， 萃取时间 120分钟， 按实 施例 1 中的方法和歩骤制取大豆浓缩蛋白。 所获得的大豆浓缩蛋白产品的干 基蛋白含量为 66wt%。 可见， 采用本发明的方法生产的大豆浓缩蛋白产品中干基蛋白质的含量 大于 60wt%， 与目前市售的大豆浓缩蛋白产品中的蛋白质含量相当， 并且， 本发明的生产大豆浓缩蛋白的方法具有缩短了工艺流程， 大幅降低了原料成 本的优点。 以上列举的仅是实现本发明的几个具体实施例。显然，本发明不限于以上 实施例， 还可以有许多变形。 本领域的普通技术人员能从本发明公开的内容 直接导出或联想到的所有变形， 均应认为是本发明的保护范围。

Claims

权利要求书
1. 一种生产大豆浓缩蛋白的方法， 包括以下步骤：
步骤 1. 以大豆为原料， 经脱皮、 轧片后， 制得大豆片， 使用己烷对所述大豆片 进行萃取， 分离萃取后的固相和液相， 所述固相即为湿豆粕；
步骤 2. 以所述湿豆粕为原料，用乙醇水溶液为萃取剂直接对所述湿豆粕进行萃 取， 之后进行固相和液相的分离；
步骤 3. 脱除上述步骤 2中所得固相中的乙醇、 己烷和水分， 得到所述大豆浓缩 蛋白。
2. 如权利要求 1所述的方法， 其中， 所述乙醇水溶液的浓度为 60〜75% (v/v) , 所 述乙醇水溶液与所述湿豆粕的重量比为 6:(1〜2)， 萃取温度为 38〜58 °C， 萃取时 间为 90〜180分钟。
3. 根据权利要求 1或 2所述的方法， 其中， 所述步骤 2中的萃取为逆流萃取。
4. 根据权利要求 1或 2所述的方法， 其中， 所述方法进一步包括将步骤 1所得液 相中的己垸蒸出， 冷凝， 并循环使用的步骤。
5. 根据权利要求 1或 2所述的方法， 其中， 所述方法进一步包括将步骤 2所得液 相中的乙醇、 己垸和水分蒸出， 冷凝， 自然分层分离， 并循环使用的步骤。
PCT/CN2011/073462 2010-04-30 2011-04-28 大豆浓缩蛋白的生产方法 WO2011134411A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN 201010163390 CN101851268A (zh) 2010-04-30 2010-04-30 湿豆粕直接制取大豆浓缩蛋白工艺
CN201010163390.6 2010-04-30

Publications (1)

Publication Number Publication Date
WO2011134411A1 true WO2011134411A1 (zh) 2011-11-03

Family

ID=42802970

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2011/073462 WO2011134411A1 (zh) 2010-04-30 2011-04-28 大豆浓缩蛋白的生产方法

Country Status (2)

Country Link
CN (1) CN101851268A (zh)
WO (1) WO2011134411A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3469917A1 (en) * 2017-10-10 2019-04-17 Rio Pardo Bioenergia SA. Production process of soybean meal with high content of soluble proteins and product so obtained

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101851268A (zh) * 2010-04-30 2010-10-06 祁鲲 湿豆粕直接制取大豆浓缩蛋白工艺
CN102696855A (zh) * 2012-05-29 2012-10-03 郑州鼎盛机械设备有限公司 利用花生粕脱糖制备花生蛋白粉的方法
CN103053796A (zh) * 2013-01-22 2013-04-24 江南大学 一种玉米酒糟(ddgs)连续浸出脱脂方法
PL3193627T5 (pl) * 2014-09-18 2023-12-04 Dsm Ip Assets B.V. Sposób wytwarzania mieszanki białek z nasion oleistych
CN115005317A (zh) * 2022-06-16 2022-09-06 中国农业科学院饲料研究所 一种棕榈仁浓缩蛋白、其制备方法及生产系统

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998800A (en) * 1973-12-26 1976-12-21 The Procter & Gamble Company Deflavoring oleaginous seed protein materials
CN101851268A (zh) * 2010-04-30 2010-10-06 祁鲲 湿豆粕直接制取大豆浓缩蛋白工艺

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3998800A (en) * 1973-12-26 1976-12-21 The Procter & Gamble Company Deflavoring oleaginous seed protein materials
CN101851268A (zh) * 2010-04-30 2010-10-06 祁鲲 湿豆粕直接制取大豆浓缩蛋白工艺

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GU, YUXING ET AL.: "Optimization of alcohol extracting process for soybean protein concentrate", FOOD SCIENCE AND TECHNOLOGY, no. 6, 20 November 1997 (1997-11-20), pages 15 - 18 *
GU, YUXING ET AL.: "Optimization of alcohol leaching process for soybean protein concentrate", CHINA OIL AND FATS, vol. 22, no. 3, 25 June 1997 (1997-06-25), pages 12 - 14 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3469917A1 (en) * 2017-10-10 2019-04-17 Rio Pardo Bioenergia SA. Production process of soybean meal with high content of soluble proteins and product so obtained

Also Published As

Publication number Publication date
CN101851268A (zh) 2010-10-06

Similar Documents

Publication Publication Date Title
WO2011134411A1 (zh) 大豆浓缩蛋白的生产方法
JPS584544B2 (ja) ヨウバイチユウシユツホウ
CN102224908A (zh) 一种黑蒜汁及黑蒜粉的制备方法
CN104087424B (zh) 一步萃取、分离牡丹鲜花精油、水溶液、脂质的方法
WO2009089030A1 (en) Dry-mill ethanol plant extraction enhancement
CN107056876A (zh) 一种从油茶籽粕中提取高纯度茶皂素的方法
CN107011988A (zh) 一种椰子油提取工艺及其椰子油
WO2015020051A1 (ja) 全果りんご及び・又はリンゴ搾汁残渣からのセラミドおよび又はペクチンの抽出方法
CN1144835A (zh) 从迷迭香植物中提取抗氧化剂的方法
JP7144270B2 (ja) 可溶性タンパク質を高含有量で含む大豆粕の生成プロセスとこれにより得られる生成物
CN102586007A (zh) 一种茶油的提取方法
CN102295987B (zh) 溶剂法从紫菜中提取紫菜油的方法
CN101311148B (zh) 一种双水相萃取精制柿果（子）番茄红素的方法
CN104740089A (zh) 一种绿茶提取物及其制备方法
RU2330430C1 (ru) Способ производства пищевых волокон
JP2002536533A (ja) 揮発性油の抽出
CN105266075A (zh) 一种利用海参漂烫液提取海参皂苷的方法
CN107446693B (zh) 一种超声波-汽液共存态乙醇流体萃取油脂的方法
CN110643433A (zh) 一种石斛花精油的提取方法
WO2015054907A1 (zh) 一种绿咖啡豆提取物及其生产方法
CN103952238A (zh) 一种沙姜提取物的制备方法
CN111154512A (zh) 脱除褐煤粗蜡中树脂的方法及从褐煤中提取褐煤蜡的方法
US10982170B2 (en) Extraction methods of field muskmelon seed oil
CN102488757A (zh) 一种提取芍药花粉黄酮的方法
WO2022045354A1 (ja) 植物ステロール原料の製造方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 11774402

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

32PN Ep: public notification in the ep bulletin as address of the adressee cannot be established

Free format text: NOTING OF LOSS OF RIGHTS PURSUANT TO RULE 112(1) EPC (EPO FORM 1205N DATED 01.03.2013)

122 Ep: pct application non-entry in european phase

Ref document number: 11774402

Country of ref document: EP

Kind code of ref document: A1