WO2011025262A3 - 결핵균군 및 마이코박테리아 속 구분 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법 - Google Patents

결핵균군 및 마이코박테리아 속 구분 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법 Download PDF

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WO2011025262A3
WO2011025262A3 PCT/KR2010/005703 KR2010005703W WO2011025262A3 WO 2011025262 A3 WO2011025262 A3 WO 2011025262A3 KR 2010005703 W KR2010005703 W KR 2010005703W WO 2011025262 A3 WO2011025262 A3 WO 2011025262A3
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mycobacterium
mycobacterium tuberculosis
composition
real
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PCT/KR2010/005703
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WO2011025262A2 (ko
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강진석
박영석
유은주
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주식회사 엘지생명과학
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Priority to JP2012526652A priority Critical patent/JP5783383B2/ja
Priority to CN201080036118.XA priority patent/CN102471806B/zh
Publication of WO2011025262A2 publication Critical patent/WO2011025262A2/ko
Publication of WO2011025262A3 publication Critical patent/WO2011025262A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
  • Biochemistry (AREA)
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  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

본 발명은 (i) 결핵균 특이적 유전자인 IS6110을 표적으로 하는 프라이머 및/또는 프로브, (ii) 마이코박테리아 속 특이적 유전자인 ITS(Inter-Transcriptional Spacer)를 표적으로 하는 프라이머 및/또는 프로브, 및 선택적으로 (iii) 내부 대조군(internal control)으로서 식물유래 유전자를 표적으로 하는 프라이머 및/또는 프로브를 포함하는 결핵균 및 마이코박테리아 속 구분 검출용 조성물을 제공한다. 본 발명에 따른 조성물을 이용하여 실시간 다중 중합효소 연쇄반응을 수행하는 경우 1회의 반응을 통해서 결핵균 뿐만 아니라 마이코박테리아 속을 검출할 수 있으므로 높은 신뢰성으로 신속하고 용이하게 임상 진단을 할 수 있다.
PCT/KR2010/005703 2009-08-26 2010-08-25 결핵균군 및 마이코박테리아 속 구분 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법 WO2011025262A2 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2012526652A JP5783383B2 (ja) 2009-08-26 2010-08-25 Ｍ．ツベルクローシス（ｍ．ｔｕｂｅｒｃｕｌｏｓｉｓ）群、およびマイコバクテリア（ｍｙｃｏｂａｃｔｅｒｉａ）属を検出するための組成物と、同組成物を用いたマルチプレックスリアルタイムｐｃｒによりｍ．ツベルクローシス（ｍ．ｔｕｂｅｒｃｕｌｏｓｉｓ）群、およびマイコバクテリア（ｍｙｃｏｂａｃｔｅｒｉａ）属を同時に検出する方法
CN201080036118.XA CN102471806B (zh) 2009-08-26 2010-08-25 检测结核性分枝杆菌复合群和分枝杆菌属的组合物以及使用该组合物用多重实时pcr同时检测结核性分枝杆菌复合群和分枝杆菌属的方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090079120A KR101458781B1 (ko) 2009-08-26 2009-08-26 결핵균군 및 마이코박테리아 속 구분 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법
KR10-2009-0079120 2009-08-26

Publications (2)

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WO2011025262A2 WO2011025262A2 (ko) 2011-03-03
WO2011025262A3 true WO2011025262A3 (ko) 2011-07-07

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PCT/KR2010/005703 WO2011025262A2 (ko) 2009-08-26 2010-08-25 결핵균군 및 마이코박테리아 속 구분 검출용 조성물 및 이를 이용한 실시간 다중 중합효소 연쇄반응에 의한 결핵균 및 마이코박테리아 속의 동시 분석 방법

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JP (1) JP5783383B2 (ko)
KR (1) KR101458781B1 (ko)
CN (1) CN102471806B (ko)
WO (1) WO2011025262A2 (ko)

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CN102808031B (zh) * 2012-08-24 2013-11-20 广东出入境检验检疫局检验检疫技术中心 检测及鉴别分枝杆菌的mPCR-DHPLC引物和方法
KR101403507B1 (ko) * 2013-03-21 2014-06-09 주식회사 현일바이오 결핵균 및 비결핵 마이코박테리아의 선택적 검출 방법, 그리고 이를 이용한 키트
US10087490B2 (en) * 2014-12-18 2018-10-02 Roche Molecular Systems, Inc. Compositions and methods for detection of drug resistant Mycobacterium tuberculosis
CN107058522A (zh) * 2017-03-20 2017-08-18 河北省胸科医院 用于检测结核分枝杆菌和非结核分枝杆菌的试剂盒及方法
WO2019031920A1 (ko) * 2017-08-11 2019-02-14 주식회사 팍스젠바이오 결핵 진단용 키트 및 이를 이용한 결핵의 진단방법
US11174520B2 (en) 2018-02-27 2021-11-16 Delta Electronics, Inc. Method for detecting presence or absence of Mycobacterium and kit thereof
TWI677578B (zh) * 2018-02-27 2019-11-21 台達電子工業股份有限公司 檢測結核分枝桿菌的方法及其套組
TWI746897B (zh) * 2018-08-08 2021-11-21 台達電子工業股份有限公司 檢測分枝桿菌的方法及其套組
KR20220098246A (ko) * 2019-12-18 2022-07-11 후지필름 가부시키가이샤 마이코박테륨·투베르쿨로시스, 마이코박테륨·아비움 및 마이코박테륨·인트라셀룰라레를 검출하기 위한 프라이머 세트 및 이것을 이용한 방법, 및 그를 위한 시약 키트
CN112725475A (zh) * 2021-01-05 2021-04-30 四川大学华西医院 一种结核分枝杆菌检测引物和探针组合物、试剂盒及应用

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KR100454585B1 (ko) * 2001-10-09 2004-11-02 주식회사 에스제이하이테크 마이코박테리아의 균주 감별, 결핵균의 스트레인 감별과항생제 내성을 검출하기 위한 프로브를 포함하는마이크로어레이와 이를 이용한 검출 방법 및 진단 키트
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Also Published As

Publication number Publication date
CN102471806A (zh) 2012-05-23
JP2013502922A (ja) 2013-01-31
KR101458781B1 (ko) 2014-11-07
CN102471806B (zh) 2015-06-03
KR20110021369A (ko) 2011-03-04
JP5783383B2 (ja) 2015-09-24
WO2011025262A2 (ko) 2011-03-03

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