WO2011004781A1 - 自動分析装置 - Google Patents
自動分析装置 Download PDFInfo
- Publication number
- WO2011004781A1 WO2011004781A1 PCT/JP2010/061369 JP2010061369W WO2011004781A1 WO 2011004781 A1 WO2011004781 A1 WO 2011004781A1 JP 2010061369 W JP2010061369 W JP 2010061369W WO 2011004781 A1 WO2011004781 A1 WO 2011004781A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- light
- scattered light
- cell
- automatic analyzer
- angle
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 48
- 230000003287 optical effect Effects 0.000 claims abstract description 23
- 210000004027 cell Anatomy 0.000 claims description 80
- 210000002421 cell wall Anatomy 0.000 claims description 8
- 239000012295 chemical reaction liquid Substances 0.000 claims description 5
- 239000011541 reaction mixture Substances 0.000 claims description 3
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 239000002245 particle Substances 0.000 abstract description 82
- 239000004816 latex Substances 0.000 abstract description 28
- 229920000126 latex Polymers 0.000 abstract description 28
- 238000006243 chemical reaction Methods 0.000 abstract description 23
- 230000035945 sensitivity Effects 0.000 abstract description 22
- 230000002546 agglutinic effect Effects 0.000 abstract 1
- 230000009087 cell motility Effects 0.000 abstract 1
- 238000009434 installation Methods 0.000 abstract 1
- 230000008859 change Effects 0.000 description 43
- 238000005259 measurement Methods 0.000 description 37
- 230000010354 integration Effects 0.000 description 16
- 230000007246 mechanism Effects 0.000 description 14
- 239000000243 solution Substances 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 7
- 238000013500 data storage Methods 0.000 description 6
- 108010074051 C-Reactive Protein Proteins 0.000 description 5
- 102100032752 C-reactive protein Human genes 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 230000007423 decrease Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000004907 flux Effects 0.000 description 3
- 229910052736 halogen Inorganic materials 0.000 description 3
- 150000002367 halogens Chemical class 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 2
- 230000004520 agglutination Effects 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000004220 aggregation Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- KMSWZEWPBIGOIU-WNQIDUERSA-N (2s)-2-aminobutanedioic acid;2-oxopentanedioic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O.OC(=O)CCC(=O)C(O)=O KMSWZEWPBIGOIU-WNQIDUERSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004931 aggregating effect Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000010876 biochemical test Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/47—Scattering, i.e. diffuse reflection
- G01N21/49—Scattering, i.e. diffuse reflection within a body or fluid
- G01N21/51—Scattering, i.e. diffuse reflection within a body or fluid inside a container, e.g. in an ampoule
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/251—Colorimeters; Construction thereof
- G01N21/253—Colorimeters; Construction thereof for batch operation, i.e. multisample apparatus
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00386—Holding samples at elevated temperature (incubation) using fluid heat transfer medium
- G01N2035/00396—Holding samples at elevated temperature (incubation) using fluid heat transfer medium where the fluid is a liquid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0439—Rotary sample carriers, i.e. carousels
- G01N2035/0453—Multiple carousels working in parallel
Definitions
- the present invention relates to a sample analyzer for analyzing the amount of components contained in a sample, for example, an automatic analyzer for analyzing the amount of components contained in blood or urine.
- a sample analyzer that analyzes the amount of components contained in a sample
- light from a light source is irradiated to the sample or a reaction mixture in which the sample and the reagent are mixed, and the resulting transmitted light of a single or multiple wavelengths is obtained.
- Automatic analyzers that measure and calculate absorbance and calculate the amount of components from the relationship between absorbance and concentration according to Lambert-Beer's law are widely used (for example, Patent Document 1).
- a number of cells holding the reaction solution are arranged in a circle on a cell disk that is repeatedly rotated and stopped, and the cell light is rotated for about 10 minutes by a pre-arranged transmitted light measurement unit. The change in absorbance over time is measured at regular time intervals.
- the automatic analyzer is equipped with a system for measuring the amount of transmitted light
- two types of reaction are used for reaction of the reaction solution: a color reaction between the substrate and the enzyme and an agglutination reaction between the antigen and the antibody.
- the former is biochemical analysis
- test items include LDH (lactate dehydrogenase), ALP (alkaline phosphatase), and AST (aspartate oxoglutarate aminoton rafenase).
- the latter is an immunoassay
- test items include CRP (C-reactive protein), IgG (immunoglobulin), RF (rheumatoid factor) and the like.
- the measurement substance measured by the latter immunoassay is required to have low blood concentration and high sensitivity.
- the reaction solution is irradiated with light and scattered into latex agglomerates.
- Higher sensitivity has been achieved with the latex immunoaggregation method in which the amount of components contained in a sample is quantified by measuring the amount of light transmitted without being transmitted.
- Patent Document 2 a system (Patent Document 2) that separates transmitted light and scattered light using a diaphragm and measures absorbance and scattered light simultaneously, or measurement of reflected scattered light in a large aggregate formed as a result of the progress of the aggregation reaction Configuration to improve accuracy on the high concentration side (Patent Document 3), measuring the average amount of each of the forward scattered light and the back scattered light using integrating spheres before and after the reaction vessel, and correcting the turbidity change due to the cell position shift
- Patent Document 4 a method (Patent Document 5), etc. that facilitates downsizing and device adjustment by arranging a fluorescence / scattered light measurement detection system on the same plane as the cell rotation direction are disclosed.
- the scattered light is detected at the scattered light receiving angle corresponding to the particle size of the latex reagent. This is very important.
- a variety of latex reagents are used in an automatic analyzer which is a general-purpose device, and the particle size of the latex particles is generally about 0.1 ⁇ m to 1.0 ⁇ m, but the particle size is not disclosed. Even if the conventional technology detects scattered light with an automatic analyzer, it does not support latex reagents of various particle sizes, so it is highly sensitive to latex reagents of any particle size. The arrangement that can be realized was not clear.
- the cell size has been reduced by reducing the amount of the reaction solution in order to reduce the reagent running cost, and the cell size is particularly reduced to an optical path length of about 5 mm and a cell width of about 2.5 mm.
- the rotational speed of the cell cannot be reduced. For this reason, the integration time in each measurement is shortened.
- the rotating cell is measured. In particular, since the amount of scattered light is smaller than the transmitted light, it is important to secure an integration time.
- Patent Document 2 enables simultaneous measurement with scattered light and transmitted light, the configuration in which the scattered light receiver is arranged corresponding to various latex particle sizes is not clear. Moreover, the scattered light of the whole circumference is obtained using a diaphragm, and the cell width and integration time are not taken into consideration.
- Patent Document 3 acquires scattered light, it is for improving the accuracy on the high concentration side, and is not effective for increasing the sensitivity at a low concentration.
- Patent Document 4 scattered light is averaged by an integrating sphere, which does not lead to high sensitivity. Furthermore, this is a system for measuring while the cell is stopped, and does not consider the cell width and integration time of a general-purpose automatic analyzer that measures while the cell is rotating.
- a plurality of light receivers are arranged in the front direction and in a plane perpendicular to the cell rotation direction so that high sensitivity can be achieved for various types of latex particle sizes.
- An automatic analyzer includes a cell disk that holds a cell containing a reaction mixture in which a sample and a reagent are mixed on the circumference, and repeatedly rotates and stops, a light source, and a light receiver.
- a scattered light measuring unit that irradiates the cell with light emitted from the light source and measures the scattered light from the reaction liquid in the cell.
- a plurality of light receivers that are disposed in a vertical plane and receive scattered light having different scattering angles.
- the angle formed by the optical axis of the irradiated light and the optical axis of the scattered light received by each light receiver when viewed from the direction perpendicular to the rotating surface of the cell disk is ⁇ 17. It is preferably within 7 °.
- One of the plurality of light receivers is arranged at a position for receiving scattered light having a scattering angle close to the transmitted light axis, and the other one receives scattered light between the first dark ring and the first bright ring. It is preferable to arrange in a position.
- the first light receiver is arranged at a position for receiving scattered light having a scattering angle of 30 ° or less, and the second light receiver receives scattered light having at least a part of the scattering angles from 30 ° to 50 °. Place it at the position you want.
- the present invention it is possible to receive scattered light at a plurality of angles while securing integration time in the automatic analyzer. This makes it possible to perform highly sensitive measurement for various types of latex reagents. As a result, it is possible to achieve high sensitivity and high accuracy in the conventional inspection items, and to expect to incorporate new inspection items. Furthermore, since detection with a diluted specimen becomes possible, the amount of sample can be reduced.
- grain with a particle size of 0.1 micrometer, and the angle dependency of the rate of change when a particle size changes 1% The figure which shows the angle dependency of the scattered light intensity angle with respect to the particle
- grains with a particle diameter of 0.4 micrometer, and the angle dependence of the rate of change when a particle size changes 1% The figure which shows the angle dependency of the scattered light intensity angle with respect to the particle
- Fig. 1 to Fig. 9 consider the scattering angle dependence of the scattered light intensity when irradiating one latex particle in water (particle size from 0.1 ⁇ m to 1.0 ⁇ m) and the low concentration in high sensitivity measurement Then, assuming the case where the amount of components contained in the sample is small and only slightly aggregates, the result of calculating the scattering angle dependence of the change rate of the scattered light intensity when the particle size of the latex particles changes by 1% is shown.
- the rate of change in this specification is defined as the value after change / value before change. That is, when there is no change, the rate of change is calculated as 1.
- the light amount change (%) is defined as (value after change ⁇ value before change) / value before change. That is, when there is no change, the light amount change (%) is calculated as 0. These are useful as simple approximations.
- the irradiation light wavelength was set to 570 nm conventionally used in the conventional transmitted light measurement. In the measurement, scattered light that has been scattered in the reaction solution and passed through the glass window is measured in the air. This calculation is based on a wide range of considerations and calculations related to scattered light theory.For example, C. F. Bohren, D. R. Huffman, Absorption and Scattering of Light by Small Particles, J. Wiley & Sons, 1983.
- FIG. 1 is a graph for latex particles having a particle size of 0.1 ⁇ m
- FIG. 2 is a graph for latex particles having a particle size of 0.2 ⁇ m
- FIG. 3 is a graph for latex particles having a particle size of 0.3 ⁇ m
- FIG. 5 is a graph for latex particles having a particle size of 0.6 ⁇ m
- FIG. 6 is a graph for latex particles having a particle size of 0.7 ⁇ m
- FIG. 7 is a graph for latex particles having a particle size of 0.8 ⁇ m
- FIG. 9 is a graph for latex particles having a particle size of 1.0 ⁇ m.
- the scattered light intensity has several peaks depending on the scattering angle.
- the scattering angle range from the scattering angle of 0 ° to the peak angle where the scattered light intensity is initially convex downward is defined as the central part, and the scattered light intensity is convex upward in the range where the scattering angle is larger than the central part.
- the angle of the peak is defined as a bright ring, and the angle of the peak that protrudes downward is defined as a dark ring, and numbers are assigned in order from the center. That is, the center, the first dark ring, the first bright ring, the second dark ring, and the second bright ring continue in order from the scattering angle of 0 °.
- a large scattering angle region in the direction in which the rate of change of the scattered light intensity when the particle diameter changes by 1% increases, as shown in FIG. 6, is an angle close to 0 ° at the center or the first dark ring and the first dark ring. It can be seen that it is located between the dark ring and the subsequent bright ring, such as between one bright ring and between the second dark ring and the second bright ring. It is possible to measure with high sensitivity by arranging a light receiver at a position where the rate of change increases.
- a scattering angle region with a large reduction amount in the direction in which the rate of change decreases is in front of the first dark ring, or between the first bright ring and the second dark ring, and the center and dark ring, or the bright ring and the following.
- High-sensitivity measurement is also possible by being positioned between the dark ring. In this way, it is possible to increase the accuracy by arranging a large number of light receivers and measuring the increase / decrease in the rate of change. Therefore, it is useful to arrange a large number of light receivers.
- FIG. 10 shows the rate of change for particles with a particle size of 0.1 ⁇ m to 0.6 ⁇ m
- FIG. 11 shows the rate of change for particles with a particle size of 0.7 ⁇ m to 1.0 ⁇ m. From FIG. 10, it is considered that for particles with a particle size of 0.1 ⁇ m to 0.6 ⁇ m, the irradiation light enters in the vicinity of 0 °, so that it is not suitable for receiving scattered light. It can be seen that measurement is advantageous for high sensitivity.
- the rate of change decreases at the central portion at an angle of 20 ° ⁇ 10 °.
- the vicinity of a scattering angle of 40 ° ⁇ 10 ° where the light receiver is located between one light ring is advantageous for high-sensitivity measurement. For this reason, in order to cope with reagents of various particle sizes, it is desirable to arrange the scattered light detectors at the central portion and at a plurality of locations between the first dark ring and the first bright ring. Specifically, by arranging the optical system so that the light receiving angle is 30 ° or less and a plurality of angles of 30 ° or more, high sensitivity can be realized for any of various reagent particle sizes.
- FIG. 12 is a view of the cell as seen from above, that is, a view as seen from a direction perpendicular to the rotation surface of a cell disk (described later with reference to FIG. 14) that rotates while holding the cell.
- 7 is a schematic view showing the positional relationship between transmitted light 16b and scattered light 16c from the center of the cell irradiated with irradiation light 16a from the left side.
- the respective light flux widths are constant in the cell, and the irradiation light is perpendicularly incident on the cell wall surface.
- the irradiation light beam width l cw is made smaller than the cell width cw because the scattered light at the cell angle becomes stray light.
- the integration time t is obtained from the following equation (1), where cw is the cell width, lw is the total luminous flux width to be considered, mw is the left and right margin from the cell wall surface, and v is the cell rotation speed.
- lw is the following formula (1) where lcw is the width of the irradiation light beam irradiated to the cell, ldw is the width of the scattered light beam, and li is the distance between the irradiation light optical axis Cx and the scattered light optical axis Dx at the cell wall surface position. It is obtained from 2).
- lw lcw / 2 + ldw / 2 + li (2) Further, l i is obtained from the following equation (3) using the cell optical path length L and the angle ⁇ between the irradiation light optical axis and the scattered light receiving optical axis when viewed from the top of the cell.
- ⁇ 0 arctan ((2cw-2vt-4mw-lcw-ldw) / L) (4) If the angle ⁇ formed by the irradiation light optical axis and the scattered light receiving optical axis is not more than ⁇ 0 satisfying Equation (4), a plurality of angles can be received even for cell width reduction.
- the cell has uneven molding, a curved surface with corners, etc., and the left and right margin mw needs to be about 0.5 mm.
- the optical path length L is approximately 5 mm as standard in transmitted light measurement. Since the cell width cw is 2.5 mm or less and the rotation speed v is approximately 100 mm / sec or more with the recent miniaturization, ⁇ 0 can be estimated to be approximately 17.7 ° from the equation (4).
- FIG. 13 is a diagram showing the relationship between transmitted light 16b and scattered light 16c when the cell is viewed from above, that is, when viewed from a direction perpendicular to the plane of rotation of the cell disk. From the above, in the case of obtaining the scattered light of a plurality of scattering angles, that the angle of the transmitted light 16b and the scattered light 16c as viewed from the cell top placing each scattered light receiving unit so that the ⁇ [psi 0 or less The scattered light at a plurality of angles can be easily obtained while securing the integration time.
- the plurality of scattered light receivers are configured to receive light that has passed through the same wall surface as the cell wall through which transmitted light passes.
- the cell wall surface In order to improve the sensitivity in the scattered light measurement, it is important to reduce the received light of the scattered light generated on the cell wall surface as much as possible. Therefore, it is necessary to make the cell wall surface a flat surface with optically little surface unevenness.
- the cell is manufactured by injection molding, and increasing the optically flat surface leads to an increase in manufacturing cost.
- the surface through which the transmitted light passes is already an optically flat surface for measuring the amount of transmitted light. Even in the case of scattered light measurement, by measuring the scattered light that has passed through the same wall as the transmitted light, Measurement is possible without increasing costs.
- the suitable reagent particle diameter differs depending on the angle at which the scattered light is measured. Therefore, for example, in the case of a measurement system that receives scattered light in the 40 ° direction, the reagent particle diameter is 0.8. In the case of a measurement system that sets to ⁇ 1.0 ⁇ m, or a measurement system that receives scattered light in the direction of 20 °, it is possible to measure with high sensitivity by setting the particle diameter of the reagent to 0.6 ⁇ m or less.
- FIG. 14 is a schematic diagram showing an example of the overall configuration of the automatic analyzer according to the present invention.
- This automatic analyzer is equipped with a scattered light measurement unit for higher sensitivity.
- the automatic analyzer mainly has three types of disks, sample disk 3, reagent disk 6 and cell disk 9, and a dispensing mechanism for moving samples and reagents between these disks, a control unit for controlling these, a measuring unit, and a measuring unit.
- An analysis unit that processes the processed data, a control data, a measurement data, a data storage unit that stores the analysis data, an input unit that inputs and outputs data from the data storage unit, and an output unit.
- a plurality of sample cups 2 containing samples 1 are arranged on the circumference of the sample disk 3.
- a plurality of reagent bottles 5 containing the reagents 4 are arranged on the reagent disk 6.
- a plurality of cells 8 are prepared on the circumference in which the sample 1 and the reagent 4 are mixed to form a reaction solution 7.
- the sample dispensing mechanism 10 moves the sample 1 from the sample cup 2 to the cell 8 by a certain amount.
- the reagent dispensing mechanism 11 moves the reagent 4 from the reagent bottle 5 to the cell 8 by a certain amount.
- the stirring unit 12 stirs and mixes the sample 1 and the reagent 4 in the cell 8.
- the cleaning unit 14 discharges the reaction solution 7 from the cell 8 after the analysis and cleans it.
- the next sample 1 is again dispensed from the sample dispensing mechanism 10 into the washed cell 8, and a new reagent 4 is dispensed from the reagent dispensing mechanism 11 and used for another reaction.
- the cell 8 is immersed in a constant temperature fluid 17 in a constant temperature bath whose temperature and flow rate are controlled, and the cell 8 and the reaction liquid 7 therein are moved in a state where the temperature is kept constant.
- Water is used as the constant temperature fluid 17 and is controlled by a constant temperature fluid control unit that controls the temperature and flow rate of the constant temperature fluid.
- the temperature is adjusted to 37 ⁇ 0.1 ° C., which is the reaction temperature.
- a transmitted light measuring unit 13 and a scattered light measuring unit 31 are provided on a part of the circumference of the cell disk.
- the transmitted light measurement unit 13 irradiates the cell 8 with light from the halogen lamp light source 15 a, splits the transmitted light 16 a with the diffraction grating 22, and then arranges photodiodes on the array.
- Light is received by the diode array 21.
- the wavelengths received were 340 nm, 405 nm, 450 nm, 480 nm, 505 nm, 546 nm, 570 nm, 600 nm, 660 nm, 700 nm, 750 nm, and 800 nm.
- the scattered light measurement unit 31 irradiates the cell 8 with the irradiation light 16a from the LED light source 15b, and receives the transmitted light 16b with the transmitted light receiver 32 as a monitor.
- the scattered light 16c was measured by the scattered light receivers 33a and 33b.
- the scattered light receiving angles ⁇ 1 and ⁇ 2 at which the scattered light receivers 33a and 33b are disposed are 20 ° and 40 °, respectively.
- Epitex L660-02V having an irradiation light wavelength of 660 nm was used as the LED light source 15b. In this configuration, the scattered light receivers are arranged at 20 ° and 40 ° positions.
- an optical system such as a fiber or a lens may be arranged at the same position, and the light may be guided to the scattered light receivers arranged at different positions.
- the scattered light receivers 33a and 33b are arranged at positions for receiving scattered light scattered downward with respect to the irradiation light 16a. However, like the scattered light receiver 33c, the scattered light receivers 33a and 33b are arranged upward with respect to the irradiation light 16a. You may arrange
- the angle of the scattered light optical axis was adjusted by monitoring the light amounts of the scattered light receiver 33a on the lower side and the scattered light receiver 33c on the upper side. That is, a separately prepared scatterer was installed at the position of the reaction liquid 7, and the scattered light receiver 33c was adjusted to 20 ° on the upper side by matching the light amounts of the scattered light receiver 33a and the scattered light receiver 33c. This facilitates angle adjustment.
- the scattered light receivers 33a, 33b, 33c and the transmitted light receiver 32 are configured to capture the same angle at the lower side ⁇ 1 and the upper side ⁇ 3, and when integrated as one unit, the scattered light receiver 33a
- the position of the entire unit such as the position of the transmitted light receiver 32
- the noise of the light source position change is measured, but by measuring the two locations of the upper side and the lower 20 ° simultaneously, the noise of the light source position change is canceled and measured. It is possible to increase the sensitivity.
- the cell used was a cell having a width of 2.5 mm and an optical path length of 5 mm, the luminous flux width was 0.5 mm for both irradiation light and scattered light, the cell rotation speed was 200 mm / sec, and the integration time was 5 msec.
- the angle ⁇ formed by the irradiation light optical axis and the scattered light receiving optical axis viewed from the upper part of the cell was 17.7 ° or less including an error in mounting accuracy. This makes it possible to receive a plurality of scattering angles while ensuring at least 2 msec as the integration time. In this embodiment, 5 msec was secured.
- sample 1 in the sample cup 2 is dispensed into the cell 8 by the sample dispensing mechanism 10.
- a predetermined amount of the reagent 4 in the reagent bottle 5 is dispensed into the cell 8 by the reagent dispensing mechanism 11.
- the sample disk 3, the reagent disk 6, and the cell disk 9 are rotationally driven by the respective drive units under the control of the control unit, and the sample cup 2, the reagent bottle 5, and the cell 8 are driven by the timing of the dispensing mechanism. Move to match.
- FIG. 14 is a simplified diagram showing only one reagent disk and reagent dispensing mechanism, but typically there are two reagent disks, a reagent dispensing mechanism, and an agitation unit.
- the transmitted light and scattered light of the reaction solution 7 are measured each time the cell disk 9 passes through the measurement positions of the transmitted light measurement unit 13 and the scattered light measurement unit 31 while the cell disk 9 is rotating, and sequentially the data storage unit via the measurement unit. Is accumulated as reaction process data. After photometry for about 10 minutes, the inside of the cell 8 is washed by the washing mechanism 14 and the next analysis is performed. Meanwhile, if necessary, another reagent 4 is added into the cell 8 by the reagent dispensing mechanism 11 and dispensed, stirred by the stirring unit 12, and further measured for a certain time. As a result, reaction process data of the reaction solution 7 having a constant time interval is stored in the data storage unit.
- the analysis unit analyzes the component amount based on the calibration curve data for each inspection item. Data necessary for control and analysis of each unit is input from the input unit to the data storage unit. The calibration curve data is held in the data storage unit. Various data, results, and alarms are output by an output unit.
- FIG. 17 shows the results of the angle dependence experiment of the rate of change of scattered light in the latex agglutination reaction in this example.
- a CRP reagent Naopia CRP, manufactured by Sekisui Chemical Co., Ltd.
- a CRP calibrator manufactured by Sekisui Chemical Co., Ltd.
- the change in the amount of transmitted light in the 0 ° direction (%) is 0.13%
- the change in the amount of light in the 20 ° direction (%) is 0.71%
- the change in the amount of light (%) is 5 times or more. It was able to be detected greatly, and it was confirmed that it was possible to detect with high sensitivity.
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Dispersion Chemistry (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
Description
またlwは、セルに照射する照射光光束幅をlcw、散乱光光束幅をldwとし、セル壁面位置における照射光光軸Cxと散乱光光軸Dxとの距離をliとすると、次の式(2)から求められる。
さらにliは、セル光路長L、及びセル上部から見た場合の照射光光軸と散乱光受光光軸とのなす角度ψを用いて次式(3)から求められる。
これらを考慮すると、セル上部から見た場合の照射光光軸と散乱光受光光軸とのなす最大角ψ0は式(1)(2)(3)より以下の式(4)で表される。
照射光光軸と散乱光受光光軸とのなす角ψは式(4)を満たすψ0以下であればセル幅低減に対しても複数の角度を受光することができる。
2 サンプルカップ
3 サンプルディスク
4 試薬
5 試薬ボトル
6 試薬ディスク
7 反応液
8 セル
9 セルディスク
10 サンプル分注機構
11 試薬分注機構
12 攪拌部
13 透過光測定部
14 洗浄部
15 光源
15a ハロゲンランプ
15b LED
16 光
16a 照射光
16b 透過光
16c 散乱光
17 恒温流体
21 フォトダイオードアレイ
22 回折格子
31 散乱光測定部
32 透過光受光器、
33a,33b,33c 散乱光受光器
Claims (6)
- サンプルと試薬とが混合した反応液を収めたセルを円周上に保持し、回転と停止を繰り返すセルディスクと、
光源と受光器とを備え、前記セルディスクの回転中に前記光源からの照射光を前記セルに照射し、前記セル中の反応液による散乱光を測定する散乱光測定部とを有し、
前記散乱光測定部は、前記セルディスクの回転による前記セルの移動方向に対して垂直な面内に配置され、それぞれ異なる散乱角度の散乱光を受光する複数の受光器を備えたことを特徴とする自動分析装置。 - 請求項1記載の自動分析装置において、前記セルディスクの回転面に垂直な方向から見たとき、前記照射光の光軸と前記受光器による散乱光受光光軸とのなす角度が±17.7°以内であることを特徴とする自動分析装置。
- 請求項1記載の自動分析装置において、前記複数の受光器は、透過光が通過するセル壁と同じセル壁を通過した散乱光を受光することを特徴とする自動分析装置。
- 請求項1記載の自動分析装置において、前記複数の受光器のうち少なくとも1つは、暗環とそれに続く明環の間の散乱光を受光することを特徴とする自動分析装置。
- 請求項4記載の自動分析装置において、前記暗環と前記明環は第1暗環及び第1明環であることを特徴とする自動分析装置。
- 請求項1記載の自動分析装置において、前記複数の受光器のうち第1の受光器を散乱角度30°以下の散乱光を受光する位置に配置し、第2の受光器を散乱角30°~50°の散乱光を受光する位置に配置したことを特徴とする自動分析装置。
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201080031159.XA CN102472710B (zh) | 2009-07-10 | 2010-07-05 | 自动分析装置 |
US13/382,316 US8852511B2 (en) | 2009-07-10 | 2010-07-05 | Automatic analyzer |
JP2011521906A JP5318206B2 (ja) | 2009-07-10 | 2010-07-05 | 自動分析装置 |
EP10797092.3A EP2453224A4 (en) | 2009-07-10 | 2010-07-05 | AUTOMATIC ANALYZER |
US14/484,384 US9023282B2 (en) | 2009-07-10 | 2014-09-12 | Automatic analyzer |
US14/670,861 US10113962B2 (en) | 2009-07-10 | 2015-03-27 | Automatic analyzer |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2009-163987 | 2009-07-10 | ||
JP2009163987 | 2009-07-10 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US13/382,316 A-371-Of-International US8852511B2 (en) | 2009-07-10 | 2010-07-05 | Automatic analyzer |
US14/484,384 Continuation US9023282B2 (en) | 2009-07-10 | 2014-09-12 | Automatic analyzer |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2011004781A1 true WO2011004781A1 (ja) | 2011-01-13 |
Family
ID=43429204
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2010/061369 WO2011004781A1 (ja) | 2009-07-10 | 2010-07-05 | 自動分析装置 |
Country Status (5)
Country | Link |
---|---|
US (3) | US8852511B2 (ja) |
EP (1) | EP2453224A4 (ja) |
JP (3) | JP5318206B2 (ja) |
CN (2) | CN102472710B (ja) |
WO (1) | WO2011004781A1 (ja) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012098946A1 (ja) * | 2011-01-17 | 2012-07-26 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
WO2012157206A1 (ja) * | 2011-05-13 | 2012-11-22 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
JP2013024746A (ja) * | 2011-07-21 | 2013-02-04 | Hitachi High-Technologies Corp | 自動分析装置 |
WO2013042524A1 (ja) * | 2011-09-20 | 2013-03-28 | 株式会社日立ハイテクノロジーズ | 自動分析装置及び分析方法 |
JP2013213831A (ja) * | 2009-07-10 | 2013-10-17 | Hitachi High-Technologies Corp | 自動分析装置 |
JP2014137319A (ja) * | 2013-01-18 | 2014-07-28 | Hitachi High-Technologies Corp | 自動分析装置 |
WO2016129029A1 (ja) * | 2015-02-09 | 2016-08-18 | 株式会社日立製作所 | 自動分析装置 |
US10976333B2 (en) | 2016-07-19 | 2021-04-13 | Hitachi High-Tech Corporation | Automatic analysis device and automatic analysis method |
WO2022196486A1 (ja) * | 2021-03-18 | 2022-09-22 | 国立研究開発法人産業技術総合研究所 | 光学スペクトルの測定方法及びその測定装置 |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101978275B (zh) | 2008-02-05 | 2015-01-07 | 普凯尔德诊断技术有限公司 | 用于鉴定生物样品中细菌的系统 |
US10288632B2 (en) | 2009-09-21 | 2019-05-14 | Pocared Diagnostics Ltd. | System for conducting the identification of bacteria in biological samples |
CN102692367B (zh) * | 2012-06-14 | 2014-02-12 | 南京中医药大学 | 纳米粒子辨识系统装置及其识别方法 |
JP5948173B2 (ja) * | 2012-07-20 | 2016-07-06 | 株式会社日立ハイテクノロジーズ | 自動分析装置及び自動分析方法 |
JP5984290B2 (ja) * | 2012-07-20 | 2016-09-06 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
CN107656085B (zh) * | 2014-07-01 | 2021-04-09 | 深圳迈瑞生物医疗电子股份有限公司 | 一种血液检测仪 |
CN108027280B (zh) | 2015-06-26 | 2021-07-06 | 雅培实验室 | 用于使得反应容器在诊断分析仪中从处理轨道运动至旋转装置的反应容器运动部件 |
JP6437390B2 (ja) * | 2015-06-30 | 2018-12-12 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
CN105445200B (zh) * | 2015-12-30 | 2018-02-16 | 山西省农业科学院植物保护研究所 | 一种判断农药纳米乳液层状液晶形成的装置及其测试方法 |
KR102462995B1 (ko) * | 2016-01-06 | 2022-11-03 | 엘지이노텍 주식회사 | 수광 모듈 및 그를 포함하는 먼지 센서 |
JP6576843B2 (ja) * | 2016-01-22 | 2019-09-18 | 株式会社日立ハイテクノロジーズ | 自動分析装置及びその散乱光測定光学系評価用標準液 |
JP7077175B2 (ja) * | 2018-08-07 | 2022-05-30 | キヤノン株式会社 | 自動分析装置、自動分析方法、および、プログラム |
JP7192675B2 (ja) * | 2019-06-26 | 2022-12-20 | 株式会社島津製作所 | 光散乱検出装置および光散乱検出方法 |
CN111504869B (zh) * | 2020-05-15 | 2021-06-08 | 中国计量科学研究院 | 流式聚集体杂质分析仪 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4451433A (en) | 1980-11-10 | 1984-05-29 | Hitachi, Ltd. | Automatic chemical analyzer |
JPH01295134A (ja) | 1988-05-24 | 1989-11-28 | Toshiba Corp | 自動化学分折装置 |
JPH0545282A (ja) * | 1991-08-09 | 1993-02-23 | Kurabo Ind Ltd | 自動臨床分析システム |
JPH0572210A (ja) * | 1991-02-05 | 1993-03-23 | Hitachi Ltd | 自動分析装置および自動分析方法 |
JPH10332582A (ja) | 1997-05-28 | 1998-12-18 | Kyowa Medex Co Ltd | 濁度測定装置 |
JP2000065744A (ja) * | 1998-08-25 | 2000-03-03 | Hitachi Ltd | 自動分析装置 |
JP2001141654A (ja) | 1999-10-08 | 2001-05-25 | Dade Behring Marburg Gmbh | 分光光度・比濁検出ユニット |
JP2005536721A (ja) * | 2002-07-19 | 2005-12-02 | エクソンモービル リサーチ アンド エンジニアリング カンパニー | 小角光散乱によって決定される石油混合物中のアスファルテン凝集 |
JP2008008794A (ja) | 2006-06-29 | 2008-01-17 | Olympus Corp | 分析装置 |
Family Cites Families (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5517440A (en) * | 1978-07-24 | 1980-02-06 | Fujitsu General Ltd | Automatic analyzer for blood biochemistry |
US4541719A (en) * | 1982-07-20 | 1985-09-17 | Wyatt Philip J | Method and apparatus for characterizing microparticles and measuring their response to their environment |
JPS5970944A (ja) * | 1982-10-15 | 1984-04-21 | Toshiba Corp | 粒径測定装置 |
US4595291A (en) | 1982-10-15 | 1986-06-17 | Tokyo Shibaura Denki Kabushiki Kaisha | Particle diameter measuring device |
JPS59100862A (ja) * | 1982-12-01 | 1984-06-11 | Hitachi Ltd | 自動分析装置 |
US4781460A (en) | 1986-01-08 | 1988-11-01 | Coulter Electronics Of New England, Inc. | System for measuring the size distribution of particles dispersed in a fluid |
JPS63205546A (ja) * | 1987-02-20 | 1988-08-25 | Nippon Tectron Co Ltd | 自動分析装置 |
JPH0187236U (ja) * | 1987-12-01 | 1989-06-08 | ||
JP2863874B2 (ja) * | 1990-12-30 | 1999-03-03 | 株式会社堀場製作所 | 粒度分布測定装置 |
JPH067054U (ja) * | 1992-06-30 | 1994-01-28 | 株式会社ニッテク | 自動分析装置における気泡検出装置 |
DE19713200C1 (de) * | 1997-03-28 | 1998-06-18 | Alv Laser Vertriebsgesellschaf | Meßgerät zur Bestimmung der statischen und/oder dynamischen Lichtstreuung |
JP3393817B2 (ja) * | 1998-10-16 | 2003-04-07 | 株式会社堀場製作所 | 粒径分布測定装置 |
JP2001174457A (ja) * | 1999-12-21 | 2001-06-29 | Matsushita Electric Ind Co Ltd | 溶液濃度計測方法、溶液濃度計測装置及び尿検査方法。 |
US6498645B1 (en) * | 2000-11-05 | 2002-12-24 | Julius Z. Knapp | Inspection of liquid injectable products for contaminating particles |
US20040016686A1 (en) * | 2002-07-24 | 2004-01-29 | Wyatt Philip J. | Absolute measurement centrifuge |
US7294513B2 (en) * | 2002-07-24 | 2007-11-13 | Wyatt Technology Corporation | Method and apparatus for characterizing solutions of small particles |
US6774994B1 (en) * | 2003-08-13 | 2004-08-10 | Wyatt Technology Corporation | Method and apparatus for determining absolute number densities of particles in suspension |
JP2008032548A (ja) * | 2006-07-28 | 2008-02-14 | Shimadzu Corp | 光散乱検出装置 |
JP2008039539A (ja) * | 2006-08-04 | 2008-02-21 | Shimadzu Corp | 光散乱検出装置 |
JP5260949B2 (ja) * | 2007-04-27 | 2013-08-14 | 古河電気工業株式会社 | 光計測装置および光計測方法 |
CN201110835Y (zh) * | 2007-09-11 | 2008-09-03 | 中国科学院上海光学精密机械研究所 | 大口径钕玻璃表面疵病的激光散射检测装置 |
CN101122555A (zh) * | 2007-09-12 | 2008-02-13 | 上海理工大学 | 基于后向光子相关光谱的高浓度超细颗粒测量装置及方法 |
EP2113564A1 (en) * | 2008-05-01 | 2009-11-04 | Arecor Limited | Protein formulation |
WO2011004781A1 (ja) * | 2009-07-10 | 2011-01-13 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
-
2010
- 2010-07-05 WO PCT/JP2010/061369 patent/WO2011004781A1/ja active Application Filing
- 2010-07-05 CN CN201080031159.XA patent/CN102472710B/zh active Active
- 2010-07-05 CN CN201410818430.4A patent/CN104535536B/zh active Active
- 2010-07-05 EP EP10797092.3A patent/EP2453224A4/en active Pending
- 2010-07-05 JP JP2011521906A patent/JP5318206B2/ja active Active
- 2010-07-05 US US13/382,316 patent/US8852511B2/en active Active
-
2013
- 2013-07-09 JP JP2013143489A patent/JP5613298B2/ja active Active
-
2014
- 2014-09-05 JP JP2014181323A patent/JP5908954B2/ja active Active
- 2014-09-12 US US14/484,384 patent/US9023282B2/en active Active
-
2015
- 2015-03-27 US US14/670,861 patent/US10113962B2/en active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4451433A (en) | 1980-11-10 | 1984-05-29 | Hitachi, Ltd. | Automatic chemical analyzer |
JPH01295134A (ja) | 1988-05-24 | 1989-11-28 | Toshiba Corp | 自動化学分折装置 |
JPH0572210A (ja) * | 1991-02-05 | 1993-03-23 | Hitachi Ltd | 自動分析装置および自動分析方法 |
JPH0545282A (ja) * | 1991-08-09 | 1993-02-23 | Kurabo Ind Ltd | 自動臨床分析システム |
JPH10332582A (ja) | 1997-05-28 | 1998-12-18 | Kyowa Medex Co Ltd | 濁度測定装置 |
JP2000065744A (ja) * | 1998-08-25 | 2000-03-03 | Hitachi Ltd | 自動分析装置 |
JP2001141654A (ja) | 1999-10-08 | 2001-05-25 | Dade Behring Marburg Gmbh | 分光光度・比濁検出ユニット |
JP2005536721A (ja) * | 2002-07-19 | 2005-12-02 | エクソンモービル リサーチ アンド エンジニアリング カンパニー | 小角光散乱によって決定される石油混合物中のアスファルテン凝集 |
JP2008008794A (ja) | 2006-06-29 | 2008-01-17 | Olympus Corp | 分析装置 |
Non-Patent Citations (2)
Title |
---|
.: ".", [KHIMIYA I TEKHNOLOGIYA VODY, vol. 7, no. 1, 1985, pages 82 - 84, XP008149130 * |
See also references of EP2453224A4 |
Cited By (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2013213831A (ja) * | 2009-07-10 | 2013-10-17 | Hitachi High-Technologies Corp | 自動分析装置 |
CN103261876A (zh) * | 2011-01-17 | 2013-08-21 | 株式会社日立高新技术 | 自动分析装置 |
JP2012149903A (ja) * | 2011-01-17 | 2012-08-09 | Hitachi High-Technologies Corp | 自動分析装置 |
US9080972B2 (en) | 2011-01-17 | 2015-07-14 | Hitachi High-Technologies Corporation | Automatic analyzer |
CN103261876B (zh) * | 2011-01-17 | 2016-01-13 | 株式会社日立高新技术 | 自动分析装置 |
WO2012098946A1 (ja) * | 2011-01-17 | 2012-07-26 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
JP2012237691A (ja) * | 2011-05-13 | 2012-12-06 | Hitachi High-Technologies Corp | 自動分析装置 |
CN103547912A (zh) * | 2011-05-13 | 2014-01-29 | 株式会社日立高新技术 | 自动分析装置 |
EP2708873A1 (en) * | 2011-05-13 | 2014-03-19 | Hitachi High-Technologies Corporation | Automatic analysis device |
US9645160B2 (en) | 2011-05-13 | 2017-05-09 | Hitachi High-Technologies Corporation | Automatic analysis device |
CN103547912B (zh) * | 2011-05-13 | 2015-11-25 | 株式会社日立高新技术 | 自动分析装置 |
EP2708873A4 (en) * | 2011-05-13 | 2014-10-08 | Hitachi High Tech Corp | AUTOMATIC ANALYSIS DEVICE |
WO2012157206A1 (ja) * | 2011-05-13 | 2012-11-22 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
JP2013024746A (ja) * | 2011-07-21 | 2013-02-04 | Hitachi High-Technologies Corp | 自動分析装置 |
WO2013042524A1 (ja) * | 2011-09-20 | 2013-03-28 | 株式会社日立ハイテクノロジーズ | 自動分析装置及び分析方法 |
US20140220705A1 (en) * | 2011-09-20 | 2014-08-07 | Hitachi High-Technologies Corporation | Automated analyzer and analyzing method |
US9664678B2 (en) | 2011-09-20 | 2017-05-30 | Hitachi High-Technologies Corporation | Automated analyzer and analyzing method |
JP2014137319A (ja) * | 2013-01-18 | 2014-07-28 | Hitachi High-Technologies Corp | 自動分析装置 |
WO2016129029A1 (ja) * | 2015-02-09 | 2016-08-18 | 株式会社日立製作所 | 自動分析装置 |
US10976333B2 (en) | 2016-07-19 | 2021-04-13 | Hitachi High-Tech Corporation | Automatic analysis device and automatic analysis method |
US11674970B2 (en) | 2016-07-19 | 2023-06-13 | Hitachi High-Tech Corporation | Automatic analysis device and automatic analysis method |
US11971425B2 (en) | 2016-07-19 | 2024-04-30 | Hitachi High-Tech Corporation | Automatic analysis device and automatic analysis method |
WO2022196486A1 (ja) * | 2021-03-18 | 2022-09-22 | 国立研究開発法人産業技術総合研究所 | 光学スペクトルの測定方法及びその測定装置 |
Also Published As
Publication number | Publication date |
---|---|
EP2453224A1 (en) | 2012-05-16 |
CN102472710A (zh) | 2012-05-23 |
CN104535536B (zh) | 2017-08-25 |
JP2015007649A (ja) | 2015-01-15 |
US8852511B2 (en) | 2014-10-07 |
US20120141330A1 (en) | 2012-06-07 |
US20140377853A1 (en) | 2014-12-25 |
CN102472710B (zh) | 2015-01-14 |
JP5613298B2 (ja) | 2014-10-22 |
JP5908954B2 (ja) | 2016-04-26 |
US20150198525A1 (en) | 2015-07-16 |
JPWO2011004781A1 (ja) | 2012-12-20 |
JP2013213831A (ja) | 2013-10-17 |
CN104535536A (zh) | 2015-04-22 |
US9023282B2 (en) | 2015-05-05 |
EP2453224A4 (en) | 2015-05-27 |
JP5318206B2 (ja) | 2013-10-16 |
US10113962B2 (en) | 2018-10-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP5908954B2 (ja) | 自動分析装置 | |
JP5379044B2 (ja) | 自動分析装置 | |
EP2759828B1 (en) | Automated analyzer and analyzing method | |
JP5296015B2 (ja) | 自動分析装置 | |
WO2014013820A1 (ja) | 自動分析装置 | |
JP5948173B2 (ja) | 自動分析装置及び自動分析方法 | |
EP2988111B1 (en) | Analyzer and automatic analyzer | |
JP6437390B2 (ja) | 自動分析装置 | |
JP6031552B2 (ja) | 自動分析装置及び分析方法 | |
JP6138564B2 (ja) | 自動分析装置 | |
JP6657016B2 (ja) | 自動分析装置 | |
WO2016129029A1 (ja) | 自動分析装置 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 201080031159.X Country of ref document: CN |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 10797092 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2011521906 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2010797092 Country of ref document: EP |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 13382316 Country of ref document: US |