WO2009060847A1 - 核酸増幅用サンプルの調製方法及び調製キット - Google Patents

核酸増幅用サンプルの調製方法及び調製キット Download PDF

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Publication number
WO2009060847A1
WO2009060847A1 PCT/JP2008/070089 JP2008070089W WO2009060847A1 WO 2009060847 A1 WO2009060847 A1 WO 2009060847A1 JP 2008070089 W JP2008070089 W JP 2008070089W WO 2009060847 A1 WO2009060847 A1 WO 2009060847A1
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
amplification
sample
biological sample
acid amplification
Prior art date
Application number
PCT/JP2008/070089
Other languages
English (en)
French (fr)
Inventor
Yasuyoshi Mori
Tsugunori Notomi
Tsuyoshi Shindome
Original Assignee
Eiken Kagaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eiken Kagaku Kabushiki Kaisha filed Critical Eiken Kagaku Kabushiki Kaisha
Priority to US12/741,322 priority Critical patent/US9347055B2/en
Priority to EP08847811.0A priority patent/EP2218791B1/en
Priority to JP2009540053A priority patent/JP5290987B2/ja
Priority to CN200880115388.2A priority patent/CN101849024B/zh
Priority to CA2703884A priority patent/CA2703884C/en
Priority to ES08847811.0T priority patent/ES2528483T3/es
Publication of WO2009060847A1 publication Critical patent/WO2009060847A1/ja
Priority to ZA2010/03126A priority patent/ZA201003126B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　生体試料及び核酸抽出液中に含まれる核酸の増幅を阻害する物質を取り除き、生体試料に含まれる標的核酸の有無及び標的遺伝子の発現量を、酵素を用いた核酸の増幅手段によって簡易かつ正確に評価できるようにすること。本発明は、生体試料に含まれる核酸の増幅に使用する核酸増幅用サンプルの調製方法であって、生体試料に核酸抽出試薬を加えて核酸抽出液を得る抽出ステップと、核酸抽出液をゼオライトに接触させることにより、より高感度の核酸検出を可能とする核酸増幅に適した核酸増幅用サンプルを得る精製ステップと、を備える調製方法を提供する。
PCT/JP2008/070089 2007-11-05 2008-11-05 核酸増幅用サンプルの調製方法及び調製キット WO2009060847A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US12/741,322 US9347055B2 (en) 2007-11-05 2008-11-05 Method and kit for preparation of sample for use in nucleic acid amplification
EP08847811.0A EP2218791B1 (en) 2007-11-05 2008-11-05 Method and kit for preparation of sample for use in nucleic acid amplification
JP2009540053A JP5290987B2 (ja) 2007-11-05 2008-11-05 核酸増幅用サンプルの調製方法及び調製キット
CN200880115388.2A CN101849024B (zh) 2007-11-05 2008-11-05 核酸扩增用样品的制备方法及制备试剂盒
CA2703884A CA2703884C (en) 2007-11-05 2008-11-05 Method and kit for preparation of sample for use in nucleic acid amplification
ES08847811.0T ES2528483T3 (es) 2007-11-05 2008-11-05 Método y kit para la preparación de una muestra para su uso en amplificación de ácido nucleico
ZA2010/03126A ZA201003126B (en) 2007-11-05 2010-05-04 Method and kit for preparation of sample for use in nucleic acid amplification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-287655 2007-11-05
JP2007287655 2007-11-05

Publications (1)

Publication Number Publication Date
WO2009060847A1 true WO2009060847A1 (ja) 2009-05-14

Family

ID=40625735

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/070089 WO2009060847A1 (ja) 2007-11-05 2008-11-05 核酸増幅用サンプルの調製方法及び調製キット

Country Status (9)

Country Link
US (1) US9347055B2 (ja)
EP (1) EP2218791B1 (ja)
JP (1) JP5290987B2 (ja)
CN (1) CN101849024B (ja)
CA (1) CA2703884C (ja)
ES (1) ES2528483T3 (ja)
RU (1) RU2453600C2 (ja)
WO (1) WO2009060847A1 (ja)
ZA (1) ZA201003126B (ja)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013004366A1 (en) 2011-07-01 2013-01-10 Qiagen Gmbh Filter module in biomolecule isolation
JP2013021959A (ja) * 2011-07-20 2013-02-04 Sony Corp 核酸抽出方法及び核酸抽出用カートリッジ
JP2014030392A (ja) * 2012-08-03 2014-02-20 Ngk Insulators Ltd 標的核酸の検出方法、検出キット
KR101365737B1 (ko) 2012-08-28 2014-02-20 한정헌 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
KR101380909B1 (ko) * 2013-03-20 2014-04-01 한국생명공학연구원 핵산 정제용 흡착제 및 그 흡착제를 이용한 정제방법
US9498737B2 (en) 2011-09-13 2016-11-22 Sony Corporation Method of purifying nucleic acids, method of extracting nucleic acids and kit for purifying nucleic acids
US10934541B2 (en) 2015-09-10 2021-03-02 Kaneka Corporation Method for separating nucleic acid from specimen containing nucleic acid and device therefor
WO2023095704A1 (ja) 2021-11-25 2023-06-01 富士フイルム株式会社 核酸検査方法および検査キット

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EP2718260B1 (en) 2011-06-08 2018-07-25 Life Technologies Corporation Design and development of novel detergents for use in pcr systems
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
BR112013032629A2 (pt) 2011-06-19 2017-08-01 Abogen Inc dispositivos, soluções e métodos para coletamento de amostra
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
AU2015226811B2 (en) 2014-03-07 2021-07-15 Dna Genotek Inc. Composition and method for stabilizing nucleic acids in biological samples
EP3452197A4 (en) 2016-05-04 2020-01-22 Children's Hospital & Research Center at Oakland FAST EXTRACTION OF NUCLEIC ACIDS FROM CLINICAL SAMPLES FOR DOWNSTREAM APPLICATIONS
JP7143596B2 (ja) * 2017-03-13 2022-09-29 東ソー株式会社 核酸抽出および増幅試薬
CN112805388A (zh) * 2018-10-09 2021-05-14 公益财团法人筑波医疗中心 微生物的检测方法
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
CN113687003B (zh) * 2021-09-14 2022-06-14 谱天（天津）生物科技有限公司 一种提高蛋白质和/或肽段组质谱鉴定数的方法

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JPH09173065A (ja) 1995-09-28 1997-07-08 Becton Dickinson & Co 核酸増幅反応の阻害を減少する方法
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JP2002345462A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 吸着バッファ及び核酸精製方法
JP2005531329A (ja) * 2002-06-28 2005-10-20 アメルシャム・バイオサイエンシーズ・アクチボラグ 沈殿剤としてポリカチオン性ポリマーを用いる核酸の単離
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JPH09173065A (ja) 1995-09-28 1997-07-08 Becton Dickinson & Co 核酸増幅反応の阻害を減少する方法
JP2002345463A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 核酸吸着バッファ及び核酸精製方法
JP2002345462A (ja) * 2001-05-24 2002-12-03 Fuji Photo Film Co Ltd 吸着バッファ及び核酸精製方法
JP2005531329A (ja) * 2002-06-28 2005-10-20 アメルシャム・バイオサイエンシーズ・アクチボラグ 沈殿剤としてポリカチオン性ポリマーを用いる核酸の単離
WO2005116209A1 (en) * 2004-05-26 2005-12-08 Keygene N.V. Method and device for the isolation of nucleic acid from nucleic acid comprising material
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013004366A1 (en) 2011-07-01 2013-01-10 Qiagen Gmbh Filter module in biomolecule isolation
JP2013021959A (ja) * 2011-07-20 2013-02-04 Sony Corp 核酸抽出方法及び核酸抽出用カートリッジ
US9498737B2 (en) 2011-09-13 2016-11-22 Sony Corporation Method of purifying nucleic acids, method of extracting nucleic acids and kit for purifying nucleic acids
US10023860B2 (en) 2011-09-13 2018-07-17 Sony Corporation Method of purifying nucleic acids and kit for purifying nucleic acids
JP2014030392A (ja) * 2012-08-03 2014-02-20 Ngk Insulators Ltd 標的核酸の検出方法、検出キット
KR101365737B1 (ko) 2012-08-28 2014-02-20 한정헌 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
JP2015526091A (ja) * 2012-08-28 2015-09-10 バイオキューブシステム カンパニーリミテッドＢｉｏ Ｃｕｂｅ Ｓｙｓｔｅｍ Ｃｏ．， Ｌｔｄ． 生物学的試料から核酸増幅反応用生物学的分子を迅速に分離するための多孔性固体相及びその用途
KR101380909B1 (ko) * 2013-03-20 2014-04-01 한국생명공학연구원 핵산 정제용 흡착제 및 그 흡착제를 이용한 정제방법
US10934541B2 (en) 2015-09-10 2021-03-02 Kaneka Corporation Method for separating nucleic acid from specimen containing nucleic acid and device therefor
WO2023095704A1 (ja) 2021-11-25 2023-06-01 富士フイルム株式会社 核酸検査方法および検査キット

Also Published As

Publication number Publication date
CA2703884A1 (en) 2009-05-14
RU2453600C2 (ru) 2012-06-20
ES2528483T3 (es) 2015-02-10
CN101849024B (zh) 2015-11-25
EP2218791B1 (en) 2015-01-07
US20110015379A1 (en) 2011-01-20
EP2218791A4 (en) 2011-04-20
CN101849024A (zh) 2010-09-29
RU2010123015A (ru) 2011-12-20
JPWO2009060847A1 (ja) 2011-03-24
US9347055B2 (en) 2016-05-24
ZA201003126B (en) 2011-04-28
JP5290987B2 (ja) 2013-09-18
EP2218791A1 (en) 2010-08-18
CA2703884C (en) 2015-03-17

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