WO2009054510A1 - 等温増幅方法およびそれに用いるｄｎａポリメラーゼ - Google Patents

等温増幅方法およびそれに用いるｄｎａポリメラーゼ Download PDF

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Publication number
WO2009054510A1
WO2009054510A1 PCT/JP2008/069371 JP2008069371W WO2009054510A1 WO 2009054510 A1 WO2009054510 A1 WO 2009054510A1 JP 2008069371 W JP2008069371 W JP 2008069371W WO 2009054510 A1 WO2009054510 A1 WO 2009054510A1
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WO
WIPO (PCT)
Prior art keywords
sequence
dna polymerase
amplification method
acid sequence
nucleic acid
Prior art date
Application number
PCT/JP2008/069371
Other languages
English (en)
French (fr)
Inventor
Yoshihide Hayashizaki
Masayoshi Itoh
Alexander Lezhava
Yoshimi Benno
Yasumasa Mitani
Hajime Kanamori
Original Assignee
Riken
Kabushiki Kaisha Dnaform
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken, Kabushiki Kaisha Dnaform filed Critical Riken
Priority to EP08842183A priority Critical patent/EP2210941A4/en
Priority to US12/739,496 priority patent/US20100221787A1/en
Priority to JP2009503353A priority patent/JP4450867B2/ja
Publication of WO2009054510A1 publication Critical patent/WO2009054510A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

　特定の等温増幅方法に適したＤＮＡポリメラーゼならびにそれを用いた等温増幅方法を提供する。下記(a)または(b)のタンパク質からなるＤＮＡポリメラーゼの存在下、等温で、下記(X)に示す第一のプライマーを用いて核酸試料中の標的核酸配列の増幅反応を行う。前記ＤＮＡポリメラーゼの使用により、前記プライマーを用いる増幅反応を、従来よりも短時間で行うことが可能となる。 (a)配列番号23で表されるアミノ酸配列からなるタンパク質、(b)配列番号25で表されるアミノ酸配列からなるタンパク質 (X)標的核酸配列の3’末端部分の配列(A)にハイブリダイズする配列(Ac’)を3’末端部分に含み且つ前記標的核酸配列において前記配列(A)よりも5’側に存在する配列(B)の相補配列(Bc)にハイブリダイズする配列(B’)を前記配列(Ac’)の5’側に含むプライマー  
PCT/JP2008/069371 2007-10-25 2008-10-24 等温増幅方法およびそれに用いるｄｎａポリメラーゼ WO2009054510A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08842183A EP2210941A4 (en) 2007-10-25 2008-10-24 ISOTHERMAL AMPLIFICATION METHOD AND DNA POLYMERASE USED IN THIS METHOD
US12/739,496 US20100221787A1 (en) 2007-10-25 2008-10-24 Isothermal amplification method and dna polymerase used in the same
JP2009503353A JP4450867B2 (ja) 2007-10-25 2008-10-24 等温増幅方法およびそれに用いる等温増幅用キット

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007277496 2007-10-25
JP2007-277496 2007-10-25

Publications (1)

Publication Number Publication Date
WO2009054510A1 true WO2009054510A1 (ja) 2009-04-30

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/069371 WO2009054510A1 (ja) 2007-10-25 2008-10-24 等温増幅方法およびそれに用いるｄｎａポリメラーゼ

Country Status (4)

Country Link
US (1) US20100221787A1 (ja)
EP (1) EP2210941A4 (ja)
JP (1) JP4450867B2 (ja)
WO (1) WO2009054510A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020013058A1 (ja) * 2018-07-13 2020-01-16 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8985074B2 (en) 2010-03-19 2015-03-24 Eaton Corporation Sensing and control of a variable valve actuation system
CA2921654A1 (en) 2013-08-30 2015-03-05 University Of Connecticut Modified epstein-barr virus dna polymerase and methods for isothermal dna amplification
US20150284716A1 (en) * 2014-02-18 2015-10-08 Board Of Regents, The University Of Texas System Method for single cell sequencing of mirnas and other cellular rnas
CN108754032B (zh) * 2018-07-19 2022-02-18 上海速创诊断产品有限公司 一种具有高特异性的等温核酸扩增体系及其应用
CN116574710A (zh) * 2023-04-23 2023-08-11 天津中合基因科技有限公司 具有链置换功能的dna聚合酶及其应用

Citations (14)

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JPH09224681A (ja) 1995-08-02 1997-09-02 New England Biolabs Inc タンパク質融合による切頭型耐熱性ｄｎａポリメラーゼの過発現及び精製
JPH10313900A (ja) 1997-05-08 1998-12-02 Becton Dickinson & Co Ｒｎａ標的配列の増幅方法
WO1999006591A1 (fr) 1997-07-31 1999-02-11 The Institute Of Physical And Chemical Research Procede de detection de mutation dans une sequence de bases
WO1999054455A1 (en) 1998-04-23 1999-10-28 Takara Shuzo Co., Ltd. Method for synthesizing dna
WO2000028082A1 (fr) 1998-11-09 2000-05-18 Eiken Kagaku Kabushiki Kaisha Procede de synthese d'acide nucleique
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WO2001030993A1 (fr) 1999-10-25 2001-05-03 Wakunaga Pharmaceutical Co., Ltd. Procede de detection d'un acide nucleique cible
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WO2002024902A1 (fr) 2000-09-19 2002-03-28 Eiken Kagaku Kabushiki Kaisha Procede permettant de synthetiser un polynucleotide
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WO2004040019A1 (ja) 2002-10-29 2004-05-13 Riken 核酸の増幅法
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WO2005063977A1 (ja) 2003-12-25 2005-07-14 Riken 核酸の増幅法およびこれを利用した変異核酸の検出法
JP3897805B2 (ja) 2003-12-25 2007-03-28 独立行政法人理化学研究所 核酸の増幅法およびこれを利用した変異核酸の検出法
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020013058A1 (ja) * 2018-07-13 2020-01-16 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体
JPWO2020013058A1 (ja) * 2018-07-13 2021-07-15 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体
JP7324200B2 (ja) 2018-07-13 2023-08-09 タカラバイオ株式会社 Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体

Also Published As

Publication number Publication date
JPWO2009054510A1 (ja) 2011-03-10
EP2210941A4 (en) 2010-11-03
US20100221787A1 (en) 2010-09-02
EP2210941A1 (en) 2010-07-28
JP4450867B2 (ja) 2010-04-14

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