WO2009038195A1 - 変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 - Google Patents

変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 Download PDF

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WO2009038195A1
WO2009038195A1 PCT/JP2008/067029 JP2008067029W WO2009038195A1 WO 2009038195 A1 WO2009038195 A1 WO 2009038195A1 JP 2008067029 W JP2008067029 W JP 2008067029W WO 2009038195 A1 WO2009038195 A1 WO 2009038195A1
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residue
amino acid
substitution
natural amino
protein
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PCT/JP2008/067029
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French (fr)
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Shigeyuki Yokoyama
Kensaku Sakamoto
Tatsuo Yanagisawa
Takatsugu Kobayashi
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Riken
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Priority to BRPI0817212-9A2A priority Critical patent/BRPI0817212A2/pt
Priority to CA2706889A priority patent/CA2706889C/en
Priority to AU2008301614A priority patent/AU2008301614A1/en
Priority to CN2008801078970A priority patent/CN101952428B/zh
Priority to EP08832011.4A priority patent/EP2192185B1/en
Priority to JP2009533208A priority patent/JP5590649B2/ja
Publication of WO2009038195A1 publication Critical patent/WO2009038195A1/ja
Priority to US12/727,037 priority patent/US8735093B2/en
Priority to US14/248,062 priority patent/US9133449B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

　重原子、セレン、反応性官能基、蛍光基、クロスリンカー等、有用な官能基を有する非天然アミノ酸として好適なリジン誘導体、特にＮε－ベンジルオキシカルボニル－リジン（Ｚ－Ｌｙｓ）誘導体を所望のタンパク質に部位特異的に導入する方法を提供する。 　配列番号２に示したピロリジル－ｔＲＮＡ合成酵素のアミノ酸配列においてピロリジン結合部位を構成する３０６位のチロシン、３０９位のロイシン、及び３４８位のシステインから選択される少なくとも１つのアミノ酸残基が置換された変異体酵素であって、当該アミノ酸置換が、 　３０６位のチロシンからグリシン又はアラニンへ、 　３０９位のロイシンからグリシン又はアラニンへ、及び 　３４８位のシステインからバリン、セリン又はアラニンへ、 　の置換である変異体ピロリジル－ｔＲＮＡ合成酵素。
PCT/JP2008/067029 2007-09-20 2008-09-19 変異体ピロリジル－ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法 WO2009038195A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BRPI0817212-9A2A BRPI0817212A2 (pt) 2007-09-20 2008-09-19 Pirrolisil-trna sintetase mutante, e método para a produção de proteína tendo aminoácido não-natural integrado nela por sua utilização
CA2706889A CA2706889C (en) 2007-09-20 2008-09-19 Mutant pyrrolysyl-trna synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
AU2008301614A AU2008301614A1 (en) 2007-09-20 2008-09-19 Mutant pyrrolysyl-TRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
CN2008801078970A CN101952428B (zh) 2007-09-20 2008-09-19 突变体吡咯赖氨酰-tRNA合成酶以及使用该酶的非天然氨基酸整合蛋白质的制造方法
EP08832011.4A EP2192185B1 (en) 2007-09-20 2008-09-19 Mutant pyrrolysyl-trna synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
JP2009533208A JP5590649B2 (ja) 2007-09-20 2008-09-19 変異体ピロリジル−ｔＲＮＡ合成酵素及びこれを用いる非天然アミノ酸組み込みタンパク質の製造方法
US12/727,037 US8735093B2 (en) 2007-09-20 2010-03-18 Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same
US14/248,062 US9133449B2 (en) 2007-09-20 2014-04-08 Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same

Applications Claiming Priority (2)

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JP2007243574 2007-09-20
JP2007-243574 2007-09-20

Related Child Applications (1)

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US12/727,037 Continuation US8735093B2 (en) 2007-09-20 2010-03-18 Mutant pyrrolysyl-tRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same

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WO2009038195A1 true WO2009038195A1 (ja) 2009-03-26

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US (2) US8735093B2 (ja)
EP (1) EP2192185B1 (ja)
JP (1) JP5590649B2 (ja)
CN (1) CN101952428B (ja)
AU (1) AU2008301614A1 (ja)
BR (1) BRPI0817212A2 (ja)
CA (1) CA2706889C (ja)
WO (1) WO2009038195A1 (ja)

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JP2013107842A (ja) * 2011-11-18 2013-06-06 Institute Of Physical & Chemical Research 新規な非天然型アミノ酸、及びその利用
JP2013522352A (ja) * 2010-03-24 2013-06-13 メディカル リサーチ カウンシル 方法
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JPWO2016148044A1 (ja) * 2015-03-13 2017-12-28 中外製薬株式会社 改変アミノアシルｔＲＮＡ合成酵素およびその用途
WO2020045656A1 (ja) 2018-08-31 2020-03-05 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素
CN111051512A (zh) * 2017-07-11 2020-04-21 辛索克斯公司 非天然核苷酸的掺入及其方法
CN115701451A (zh) * 2021-08-02 2023-02-10 宁波鲲鹏生物科技有限公司 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用
JP7383306B2 (ja) 2019-03-19 2023-11-20 ニンポー クンペン バイオテック カンパニー，リミテッド タンパク質に効率的にリジン誘導体を導入するアミノアシル－ｔＲＮＡ合成酵素

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JP2013107842A (ja) * 2011-11-18 2013-06-06 Institute Of Physical & Chemical Research 新規な非天然型アミノ酸、及びその利用
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JP7325341B2 (ja) 2017-07-11 2023-08-14 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
US11834689B2 (en) 2017-07-11 2023-12-05 The Scripps Research Institute Incorporation of unnatural nucleotides and methods thereof
WO2020045656A1 (ja) 2018-08-31 2020-03-05 国立研究開発法人理化学研究所 ピロリシルｔＲＮＡ合成酵素
JP7383306B2 (ja) 2019-03-19 2023-11-20 ニンポー クンペン バイオテック カンパニー，リミテッド タンパク質に効率的にリジン誘導体を導入するアミノアシル－ｔＲＮＡ合成酵素
CN115701451A (zh) * 2021-08-02 2023-02-10 宁波鲲鹏生物科技有限公司 高效引入赖氨酸衍生物的氨酰基-tRNA合成酶及其应用

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AU2008301614A8 (en) 2010-06-03
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BRPI0817212A2 (pt) 2014-10-14
JP5590649B2 (ja) 2014-09-17
US9133449B2 (en) 2015-09-15
AU2008301614A2 (en) 2010-05-27
CA2706889C (en) 2018-08-28
CN101952428B (zh) 2013-10-23
JPWO2009038195A1 (ja) 2011-01-13
EP2192185A4 (en) 2010-09-22
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