WO2009028218A1 - 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出 - Google Patents

細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出 Download PDF

Info

Publication number
WO2009028218A1
WO2009028218A1 PCT/JP2008/053215 JP2008053215W WO2009028218A1 WO 2009028218 A1 WO2009028218 A1 WO 2009028218A1 JP 2008053215 W JP2008053215 W JP 2008053215W WO 2009028218 A1 WO2009028218 A1 WO 2009028218A1
Authority
WO
WIPO (PCT)
Prior art keywords
bacteria belonging
genus campylobacter
bacteria
detection
multiplex pcr
Prior art date
Application number
PCT/JP2008/053215
Other languages
English (en)
French (fr)
Inventor
Shinji Yamasaki
Masahiro Asakura
Original Assignee
Osaka Prefecture University
Fuso Pharmaceutical Industries, Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Osaka Prefecture University, Fuso Pharmaceutical Industries, Ltd. filed Critical Osaka Prefecture University
Priority to CA2718214A priority Critical patent/CA2718214C/en
Priority to AU2008292946A priority patent/AU2008292946B2/en
Publication of WO2009028218A1 publication Critical patent/WO2009028218A1/ja
Priority to US12/711,129 priority patent/US9200330B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　C. ジェジュニ、C. コリ、およびC. フィータスのcdt遺伝子を菌種特異的に増幅できるマルチプレックスPCR用プライマーを作製し、カンピロバクター属細菌および他のcdt遺伝子陽性細菌、代表的な腸管感染症起因菌を用いてマルチプレックスPCRの評価を行った。その結果、本発明者等によるcdtB増幅用プライマーを用いたマルチプレックスPCRは、高い特異性をもって、複数のカンピロバクター属細菌を同時に検出可能なことが証明された。本発明の方法は、家畜やヒトが複数のカンピロバクター属細菌種に混合感染している場合に、一回の操作によって菌種レベルでカンピロバクターを同定できる。
PCT/JP2008/053215 2007-08-24 2008-02-26 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出 WO2009028218A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA2718214A CA2718214C (en) 2007-08-24 2008-02-26 Detection of bacteria belonging to the genus campylobacter by targeting cytolethal distending toxin
AU2008292946A AU2008292946B2 (en) 2007-08-24 2008-02-26 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin
US12/711,129 US9200330B2 (en) 2007-08-24 2010-02-23 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-218962 2007-08-24
JP2007218962 2007-08-24

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/711,129 Continuation-In-Part US9200330B2 (en) 2007-08-24 2010-02-23 Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin

Publications (1)

Publication Number Publication Date
WO2009028218A1 true WO2009028218A1 (ja) 2009-03-05

Family

ID=40386950

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/053215 WO2009028218A1 (ja) 2007-08-24 2008-02-26 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出

Country Status (4)

Country Link
US (1) US9200330B2 (ja)
AU (1) AU2008292946B2 (ja)
CA (1) CA2718214C (ja)
WO (1) WO2009028218A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150184230A1 (en) * 2007-08-31 2015-07-02 Fuso Pharmaceutical Industries, Ltd. Detection of bacteria belonging to the genus campylobacter by targeting cytolethal distending toxin

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2903388C (en) 2003-12-05 2018-01-16 Fuso Pharmaceutical Industries, Ltd. Cytolethal distending toxins and detection of campylobacter bacteria using the same as a target
CN108913794B (zh) * 2018-08-17 2023-08-22 河南省农业科学院畜牧兽医研究所 弯曲菌细胞膨胀致死毒素a型，b型和c型三重pcr检测引物及试剂盒
KR102407237B1 (ko) * 2020-11-02 2022-06-13 주식회사 세니젠 캠필로박터를 선택적으로 구분 검출할 수 있는 리얼-타임 pcr 키트
CN113481200A (zh) * 2021-08-27 2021-10-08 安徽农业大学 一种鸭线粒体coi基因扩增引物及其应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3785590T2 (de) 1986-01-22 1993-09-16 Amoco Corp Campylobacter-sonde.
US5447848A (en) 1986-01-22 1995-09-05 Amoco Corporation Detection of campylobacter with nucleic acid probes
FR2633941A1 (fr) 1988-07-05 1990-01-12 Ire Medgenix Sa Sondes d'acides nucleiques utiles pour detecter de maniere specifique differentes especes bacteriennes du genre campylobacter
JPH03112498A (ja) 1989-09-27 1991-05-14 Shimadzu Corp カンピロバクター検出のためのオリゴヌクレオチドおよびそれを用いた検出方法
JP3112498B2 (ja) 1991-05-17 2000-11-27 日本ジーイープラスチックス株式会社 ポリエステルカーボネートの製造方法
JPH0690795A (ja) 1992-01-24 1994-04-05 Toagosei Chem Ind Co Ltd カンピロバクター ジェジュニ検出用プローブ及び検出方法
JPH0690796A (ja) 1992-01-28 1994-04-05 Toagosei Chem Ind Co Ltd カンピロバクター ジェジュニ及びコリ検出用プローブ及び検出方法
JPH05276999A (ja) 1992-04-02 1993-10-26 Toyobo Co Ltd カンピロバクター属細菌検出用オリゴヌクレオチド、カンピロバクター属細菌の検出法及び検出用試薬キット
FR2701028B1 (fr) * 1993-01-29 1995-04-21 Pasteur Institut Séquences oligonucléotidiques hybridant spécifiquement avec une séquence génonique de campylobacter jejuni. Applications comme sondes et amorces oligonucléotidiques.
FR2726826B1 (fr) 1994-11-14 1997-01-31 Pasteur Institut Sequences nucleotidiques hybridant specifiquement avec une sequence nucleique genomique de campylobacter coli, application dans le diagnostic d'une infection a campylobacter coli
JP2875632B2 (ja) 1994-12-27 1999-03-31 モトローラ・インコーポレーテッド サイマルカスト無線通信システムにおいて送信機を識別するための方法および装置
AU6715198A (en) 1997-03-25 1998-10-20 Her Majesty In Right Of Canada, As Represented By The Minister Of Health And Welfare, Canada A porin gene from (campylobacter jejuni), related products and uses thereof
US6066461A (en) 1999-04-12 2000-05-23 Becton Dickinson And Company Amplification and detection of Campylobacter jejuni and Campylobacter coli
US7205104B2 (en) 2000-03-24 2007-04-17 Eppendorf Array Technologies Sa (Eat) Identification of biological (micro) organisms by detection of their homologous nucleotide sequences on arrays
US20080261220A1 (en) * 2000-11-30 2008-10-23 Third Wave Technologies, Inc. Nucleic Acid Detection Assays
US7595386B2 (en) * 2003-01-24 2009-09-29 William Beaumont Hospital Methods and compositions for heat activated gene therapy using cytolethal distending toxin
CA2903388C (en) * 2003-12-05 2018-01-16 Fuso Pharmaceutical Industries, Ltd. Cytolethal distending toxins and detection of campylobacter bacteria using the same as a target
JP2006087406A (ja) 2004-09-27 2006-04-06 Japan Health Science Foundation ビブリオ属（Ｖｉｂｒｉｏ）細菌種の総菌数測定方法
US20090035767A1 (en) 2006-11-28 2009-02-05 Canon Kabushiki Kaisha Primer for bacterium genome amplification reaction
JP5276999B2 (ja) 2009-01-19 2013-08-28 矢崎総業株式会社 ケースの固定構造

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ASAKURA M. ET AL.: "Comparative analysis of cytoethal distending toxin (cdt) genes among Campylobacter jejuni, C.coli and C. fetus strains", MICROB.PATHOG., vol. 42, 6 February 2007 (2007-02-06), pages 174 - 183, XP022057009, DOI: doi:10.1016/j.micpath.2007.01.005 *
SACHI SHIRAMARU ET AL.: "Shu tokuiteki Cytoethal distending toxin idenshi ni motozuku nested-multiplex PCR o mochiita campylobacter-zoku saikin no jinsoku kenshutsu", JAPANESE SOCIETY OF VETERIANY SCIENCE GAKUJUTSU KOEN YOSHISHU, vol. 143, 15 March 2007 (2007-03-15), pages 201 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150184230A1 (en) * 2007-08-31 2015-07-02 Fuso Pharmaceutical Industries, Ltd. Detection of bacteria belonging to the genus campylobacter by targeting cytolethal distending toxin
US9663828B2 (en) * 2007-08-31 2017-05-30 Osaka Prefecture University Public Corporation Detection of bacteria belonging to the genus Campylobacter by targeting cytolethal distending toxin

Also Published As

Publication number Publication date
CA2718214C (en) 2018-09-11
AU2008292946A1 (en) 2009-03-05
CA2718214A1 (en) 2009-03-05
AU2008292946B2 (en) 2014-06-05
AU2008292946A2 (en) 2010-05-06
US9200330B2 (en) 2015-12-01
US20100248238A1 (en) 2010-09-30

Similar Documents

Publication Publication Date Title
JP5830461B2 (ja) Ｐｃｒ用プライマーセット、ｐｃｒ用反応液、食中毒菌の検出方法
Holman et al. Effect of co-composting cattle manure with construction and demolition waste on the archaeal, bacterial, and fungal microbiota, and on antimicrobial resistance determinants
Panangala et al. Multiplex-PCR for simultaneous detection of 3 bacterial fish pathogens, Flavobacterium columnare, Edwardsiella ictaluri, and Aeromonas hydrophila
WO2009028218A1 (ja) 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出
Gordillo et al. Quantification of viable Escherichia coli O157: H7 in meat products by duplex real-time PCR assays
Usui et al. Survival and prevalence of Clostridium difficile in manure compost derived from pigs
WO2009102896A3 (en) Isothermal nucleic acid amplification methods and compositions
EP3249056A3 (en) Isothermal nucleic acid amplification
WO2008070352A3 (en) Efficient arrays of amplified polynucleotides
WO2008122002A3 (en) Organism identification panel
Bui et al. Reverse transcriptase real-time PCR for detection and quantification of viable Campylobacter jejuni directly from poultry faecal samples
Klocke et al. Monitoring Lactobacillus plantarum in grass silages with the aid of 16S rDNA-based quantitative real-time PCR assays
BRPI0816047A2 (pt) conjunto de oligonucleotídeos para detecção de um ácido nucleico alvo, métodos para detecção da presença ou ausência de um ácido nucleico alvo e de pelo menos dois ácidos nucleicos alvo, kit, mistura de reação e amplicon identificado por fluorescência
CN101613766B (zh) 一种小反刍兽疫病毒的rt-lamp试剂盒
WO2008117782A1 (ja) 遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途
WO2011066330A3 (en) Selective amplification of polynucleotide sequences
WO2004068112A3 (en) Hairpin primer amplification
Mrazek et al. Diet-dependent shifts in ruminal butyrate-producing bacteria
Khammar et al. Use of the frc gene as a molecular marker to characterize oxalate-oxidizing bacterial abundance and diversity structure in soil
Karimnasab et al. An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments
WO2009028662A1 (ja) 細胞膨張化致死毒を標的としたカンピロバクター属細菌の検出
SG169223A1 (en) Method of detecting acid-fast bacteria using ribosomal rna as target
CN102732599A (zh) 双重聚合酶链反应方法检测海水样品中的创伤弧菌和副溶血弧菌
NZ586923A (en) Methods and oligonucleotides for detection of mastitis causing bacteria
Salihah et al. A novel, rapid, and sensitive real-time PCR assay for cost-effective detection and quantification of Staphylococcus aureus in food samples with the ZEN™ doublequenched probe chemistry

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08711944

Country of ref document: EP

Kind code of ref document: A1

WWE Wipo information: entry into national phase

Ref document number: 2718214

Country of ref document: CA

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 2008292946

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2008292946

Country of ref document: AU

Date of ref document: 20080226

Kind code of ref document: A

122 Ep: pct application non-entry in european phase

Ref document number: 08711944

Country of ref document: EP

Kind code of ref document: A1