WO2008117782A1 - 遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途 - Google Patents

遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途 Download PDF

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Publication number
WO2008117782A1
WO2008117782A1 PCT/JP2008/055469 JP2008055469W WO2008117782A1 WO 2008117782 A1 WO2008117782 A1 WO 2008117782A1 JP 2008055469 W JP2008055469 W JP 2008055469W WO 2008117782 A1 WO2008117782 A1 WO 2008117782A1
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WO
WIPO (PCT)
Prior art keywords
gene amplification
gene
primer set
same
reagent containing
Prior art date
Application number
PCT/JP2008/055469
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English (en)
French (fr)
Inventor
Mitsuharu Hirai
Satoshi Majima
Original Assignee
Arkray, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray, Inc. filed Critical Arkray, Inc.
Priority to US12/306,370 priority Critical patent/US8232051B2/en
Priority to JP2008530268A priority patent/JP5224526B2/ja
Priority to CN2008800012904A priority patent/CN101568646B/zh
Priority to KR1020087025612A priority patent/KR101135829B1/ko
Priority to EP08722735A priority patent/EP2128273B1/en
Publication of WO2008117782A1 publication Critical patent/WO2008117782A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　遺伝子増幅法により、２つの遺伝子（ＣＹＰ２Ｃ９遺伝子およびＶＫＯＲＣ１遺伝子）を増幅するためのプライマーセットであって、同一反応系において、同時に、前記３つの遺伝子の各目的領域を特異的に効率良く増幅することができるプライマーセットを提供する。 　配列番号５および配列番号２９の塩基配列からなるフォワードプライマーと、配列番号１８および配列番号３８の塩基配列からなるリバースプライマーとをそれぞれ含む２対のプライマーセットを使用する。このプライマーセットを使用することにより、同一反応液において、同時に、ＣＹＰ２Ｃ９遺伝子およびＶＫＯＲＣ１遺伝子において検出目的の多型が生じる部位を含む目的領域を、特異的に増幅することができる。
PCT/JP2008/055469 2007-03-26 2008-03-24 遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途 WO2008117782A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/306,370 US8232051B2 (en) 2007-03-26 2008-03-24 Primer set for gene amplification, reagent for gene amplification including the same, and uses thereof
JP2008530268A JP5224526B2 (ja) 2007-03-26 2008-03-24 遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途
CN2008800012904A CN101568646B (zh) 2007-03-26 2008-03-24 基因扩增用引物对、含有其的基因扩增用试剂及其用途
KR1020087025612A KR101135829B1 (ko) 2007-03-26 2008-03-24 유전자 증폭용 프라이머 세트, 그것을 포함하는 유전자 증폭용 시약 및 그 용도
EP08722735A EP2128273B1 (en) 2007-03-26 2008-03-24 Primer set for gene amplification, gene amplification reagent containing the same and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-079403 2007-03-26
JP2007079403 2007-03-26

Publications (1)

Publication Number Publication Date
WO2008117782A1 true WO2008117782A1 (ja) 2008-10-02

Family

ID=39788517

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Application Number Title Priority Date Filing Date
PCT/JP2008/055469 WO2008117782A1 (ja) 2007-03-26 2008-03-24 遺伝子増幅用プライマーセット、それを含む遺伝子増幅用試薬およびその用途

Country Status (6)

Country Link
US (1) US8232051B2 (ja)
EP (1) EP2128273B1 (ja)
JP (1) JP5224526B2 (ja)
KR (1) KR101135829B1 (ja)
CN (1) CN101568646B (ja)
WO (1) WO2008117782A1 (ja)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101899519A (zh) * 2010-07-23 2010-12-01 苏州大学 一种用于华法林个体化用药相关基因snp位点检测的试剂盒及其pcr扩增方法
EP2392676A1 (en) 2010-06-01 2011-12-07 ARKRAY, Inc. Method for detecting polymorphism at nucleotide position -1639 of VKORC1 gene, and nucleic acid probe and kit therefor
US20120214166A1 (en) * 2009-10-30 2012-08-23 Arkray, Inc. Probe for Detecting Polymorphism in EGFR Gene and Use of the Probe
JP2013009669A (ja) * 2011-05-31 2013-01-17 Arkray Inc 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、１種類の波長で複数の遺伝子多型を検出する方法

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2514841A1 (en) * 2008-01-25 2012-10-24 Theranostics Laboratory Methods and compositions for the assessment of drug response
JP6153758B2 (ja) * 2012-04-20 2017-06-28 アークレイ株式会社 多型検出用プローブ、多型検出方法、薬効判定方法及び多型検出用キット
TWI600766B (zh) 2012-08-09 2017-10-01 財團法人工業技術研究院 用於偵測一目標核苷酸序列中之一特定區域的一突變及／或多形性的套組
CN103525911A (zh) * 2013-09-22 2014-01-22 上海中优医药高科技有限公司 利用高分辨熔解曲线分析技术检测华法林个体化用药相关基因多态性的方法
JP6501594B2 (ja) * 2015-04-03 2019-04-17 住友化学株式会社 ニトロバクター（Ｎｉｔｒｏｂａｃｔｅｒ）属細菌の検出方法及びキット
CN109457026A (zh) * 2018-10-22 2019-03-12 江苏美因康生物科技有限公司 一种快速检测抗血栓个体化用药基因多态性的试剂盒及方法

Citations (5)

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US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1991009950A1 (en) 1989-12-22 1991-07-11 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
EP0455430A2 (en) 1990-04-26 1991-11-06 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
WO1992009689A1 (en) 1990-12-03 1992-06-11 Stratagene PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)

Family Cites Families (1)

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WO1999015704A1 (en) * 1997-09-23 1999-04-01 Oncormed, Inc. Genetic panel assay for susceptibility mutations in breast and ovarian cancer

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
WO1991009950A1 (en) 1989-12-22 1991-07-11 F. Hoffmann-La Roche Ag Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
EP0455430A2 (en) 1990-04-26 1991-11-06 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis
WO1992009689A1 (en) 1990-12-03 1992-06-11 Stratagene PURIFIED THERMOSTABLE $i(PYROCOCCUS FURIOSUS)

Non-Patent Citations (4)

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Title
MARK J. RIEDER ET AL., THE NEW ENGLAND JOURNAL OF MEDICINE, vol. 352, 2005, pages 22
See also references of EP2128273A4 *
SIMONE ROST ET AL., NATURE, vol. 427, 2004
TAKAHASHI H.: "Warfarin Toyoryo no Kojinsa ni Kakawaru Idenshi Kensa", KENSA TO GIJUTSU, vol. 34, no. 10, 2006, pages 984 - 986, XP008104320 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120214166A1 (en) * 2009-10-30 2012-08-23 Arkray, Inc. Probe for Detecting Polymorphism in EGFR Gene and Use of the Probe
US8748095B2 (en) * 2009-10-30 2014-06-10 Arkray, Inc. Probe for detecting polymorphism in EGFR gene and use of the probe
EP2392676A1 (en) 2010-06-01 2011-12-07 ARKRAY, Inc. Method for detecting polymorphism at nucleotide position -1639 of VKORC1 gene, and nucleic acid probe and kit therefor
US20110300539A1 (en) * 2010-06-01 2011-12-08 Arkray, Inc. Method for Detecting Polymorphism at Nucleotide Position -1639 of VKORC1 Gene, and Nucleic Acid Probe and Kit Therefor
JP2012010698A (ja) * 2010-06-01 2012-01-19 Arkray Inc Ｖｋｏｒｃ１遺伝子の−１６３９番目の塩基の多型検出法、ならびにそのための核酸プローブおよびキット
US8758997B2 (en) 2010-06-01 2014-06-24 Arkray, Inc. Method for detecting polymorphism at nucleotide position-1639 of VKORC1 gene, and nucleic acid probe and kit therefor
CN101899519A (zh) * 2010-07-23 2010-12-01 苏州大学 一种用于华法林个体化用药相关基因snp位点检测的试剂盒及其pcr扩增方法
CN101899519B (zh) * 2010-07-23 2012-07-11 苏州大学 一种用于华法林个体化用药相关基因snp位点检测的试剂盒及其pcr扩增方法
JP2013009669A (ja) * 2011-05-31 2013-01-17 Arkray Inc 同一又は近傍の検出波長を有する蛍光色素で修飾された複数のオリゴヌクレオチドを用いて、１種類の波長で複数の遺伝子多型を検出する方法

Also Published As

Publication number Publication date
CN101568646A (zh) 2009-10-28
EP2128273A1 (en) 2009-12-02
CN101568646B (zh) 2013-02-27
EP2128273A4 (en) 2010-08-04
KR20090012222A (ko) 2009-02-02
KR101135829B1 (ko) 2012-05-25
US20090233288A1 (en) 2009-09-17
EP2128273B1 (en) 2012-11-28
JPWO2008117782A1 (ja) 2010-07-15
JP5224526B2 (ja) 2013-07-03
US8232051B2 (en) 2012-07-31

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