WO2009020144A1 - 異種タンパク質の製造方法 - Google Patents

異種タンパク質の製造方法 Download PDF

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Publication number
WO2009020144A1
WO2009020144A1 PCT/JP2008/064095 JP2008064095W WO2009020144A1 WO 2009020144 A1 WO2009020144 A1 WO 2009020144A1 JP 2008064095 W JP2008064095 W JP 2008064095W WO 2009020144 A1 WO2009020144 A1 WO 2009020144A1
Authority
WO
WIPO (PCT)
Prior art keywords
heterogeneous protein
producing
producing heterogeneous
protein
polypeptide
Prior art date
Application number
PCT/JP2008/064095
Other languages
English (en)
French (fr)
Inventor
Hisahiro Tabuchi
Tomoya Sugiyama
Original Assignee
Chugai Seiyaku Kabushiki Kaisha
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to BRPI0815036-2A priority Critical patent/BRPI0815036B1/pt
Priority to EP08792251.4A priority patent/EP2186905B1/en
Priority to CN200880101621.1A priority patent/CN101809161B/zh
Priority to JP2009526472A priority patent/JP5337033B2/ja
Application filed by Chugai Seiyaku Kabushiki Kaisha filed Critical Chugai Seiyaku Kabushiki Kaisha
Priority to ES08792251.4T priority patent/ES2591284T3/es
Priority to MX2010001319A priority patent/MX2010001319A/es
Priority to AU2008284753A priority patent/AU2008284753B2/en
Priority to DK08792251.4T priority patent/DK2186905T3/en
Priority to US12/733,052 priority patent/US8697397B2/en
Priority to RU2010108308/10A priority patent/RU2487168C2/ru
Priority to CA2695849A priority patent/CA2695849C/en
Publication of WO2009020144A1 publication Critical patent/WO2009020144A1/ja
Priority to HK10110028.3A priority patent/HK1143608A1/xx
Priority to US14/225,833 priority patent/US9284374B2/en
Priority to US14/800,924 priority patent/US9499618B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01002—Alanine transaminase (2.6.1.2), i.e. alanine-aminotransferase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Abstract

　天然型タンパク質又は組換えタンパク質を高生産量で製造することができる方法を提供する。 　アラニンアミノトランスフェラーゼを強発現し、且つ所望のポリペプチドをコードするＤＮＡを導入した細胞を培養し、所望のポリペプチドを産生させることを含む、ポリペプチドの製造方法。
PCT/JP2008/064095 2007-08-07 2008-08-06 異種タンパク質の製造方法 WO2009020144A1 (ja)

Priority Applications (14)

Application Number Priority Date Filing Date Title
MX2010001319A MX2010001319A (es) 2007-08-07 2008-08-06 Metodo para producir proteinas heterogeneas.
CN200880101621.1A CN101809161B (zh) 2007-08-07 2008-08-06 异种蛋白的制备方法
JP2009526472A JP5337033B2 (ja) 2007-08-07 2008-08-06 異種タンパク質の製造方法
DK08792251.4T DK2186905T3 (en) 2007-08-07 2008-08-06 Method of Preparation of Heterogeneous Protein
ES08792251.4T ES2591284T3 (es) 2007-08-07 2008-08-06 Método para la producción de proteínas heterogéneas
EP08792251.4A EP2186905B1 (en) 2007-08-07 2008-08-06 Method of producing heterogeneous protein
AU2008284753A AU2008284753B2 (en) 2007-08-07 2008-08-06 Method of producing heterogeneous protein
BRPI0815036-2A BRPI0815036B1 (pt) 2007-08-07 2008-08-06 Métodos para produção de um polipeptídeo e para preparação de um produto farmacêutico
US12/733,052 US8697397B2 (en) 2007-08-07 2008-08-06 Method of producing heterogeneous protein
RU2010108308/10A RU2487168C2 (ru) 2007-08-07 2008-08-06 Способ получения гетерологичных белков
CA2695849A CA2695849C (en) 2007-08-07 2008-08-06 Method of producing heterogeneous proteins
HK10110028.3A HK1143608A1 (en) 2007-08-07 2010-10-25 Method of producing heterogeneous protein
US14/225,833 US9284374B2 (en) 2007-08-07 2014-03-26 Method of producing heterogeneous protein
US14/800,924 US9499618B2 (en) 2007-08-07 2015-07-16 Method of producing heterogeneous protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007205158 2007-08-07
JP2007-205158 2007-08-07

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/733,052 A-371-Of-International US8697397B2 (en) 2007-08-07 2008-08-06 Method of producing heterogeneous protein
US14/225,833 Division US9284374B2 (en) 2007-08-07 2014-03-26 Method of producing heterogeneous protein

Publications (1)

Publication Number Publication Date
WO2009020144A1 true WO2009020144A1 (ja) 2009-02-12

Family

ID=40341376

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/064095 WO2009020144A1 (ja) 2007-08-07 2008-08-06 異種タンパク質の製造方法

Country Status (15)

Country Link
US (3) US8697397B2 (ja)
EP (1) EP2186905B1 (ja)
JP (1) JP5337033B2 (ja)
KR (1) KR101577839B1 (ja)
CN (1) CN101809161B (ja)
AU (1) AU2008284753B2 (ja)
BR (1) BRPI0815036B1 (ja)
CA (1) CA2695849C (ja)
DK (1) DK2186905T3 (ja)
ES (1) ES2591284T3 (ja)
HK (1) HK1143608A1 (ja)
MX (1) MX2010001319A (ja)
RU (1) RU2487168C2 (ja)
TW (1) TWI463013B (ja)
WO (1) WO2009020144A1 (ja)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010123014A1 (ja) 2009-04-22 2010-10-28 中外製薬株式会社 異種タンパク質を高生産する細胞の作製方法
WO2012137683A1 (ja) 2011-04-01 2012-10-11 中外製薬株式会社 組換えポリペプチドの製造方法
US8697397B2 (en) 2007-08-07 2014-04-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
WO2014058025A1 (ja) 2012-10-10 2014-04-17 中外製薬株式会社 改変宿主細胞の樹立方法
US8796007B2 (en) 2007-03-15 2014-08-05 Chugai Seiyaku Kabushiki Kaisha Method for production of polypeptide
US9068212B2 (en) 2007-10-24 2015-06-30 Chugai Seiyaku Kabushiki Kaisha Method for producing a polypeptide using a cell that overexpresses a bicarbonate transporter
US9238687B2 (en) 2006-04-13 2016-01-19 Chugai Seiyaku Kabushiki Kaisha Method for recombinant production of a desired polypeptide using a mammalian cell co-expressing a taurine transporter polypeptide
JP2016514461A (ja) * 2013-03-15 2016-05-23 ジェネンテック， インコーポレイテッド 抗酸化剤を含む細胞培養組成物およびポリペプチド産生のための方法
US9802993B2 (en) 2007-10-15 2017-10-31 Chugai Seiyaku Kabushiki Kaisha Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate
WO2020122049A1 (ja) 2018-12-11 2020-06-18 富士フイルム株式会社 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法
US10927342B2 (en) 2015-08-04 2021-02-23 Regeneran Pharmaceuticals, Inc. Taurine supplemented cell culture medium and methods of use

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WO2011150241A2 (en) 2010-05-28 2011-12-01 Genentech, Inc. Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase
CN106029691A (zh) 2013-12-20 2016-10-12 诺华股份有限公司 用于重组表达感兴趣产物的新型真核细胞和方法
WO2015092735A1 (en) 2013-12-20 2015-06-25 Novartis Ag Novel eukaryotic cells and methods for recombinantly expressing a product of interest
CN103923872B (zh) * 2014-04-17 2015-11-18 湖北工业大学 一种米曲霉活力强化剂及其培养方法
CN106233219B (zh) * 2015-03-31 2020-03-17 深圳市大疆创新科技有限公司 移动平台操作系统及方法
KR102479900B1 (ko) * 2020-12-11 2022-12-26 대한민국 내염성 증진 신품종 식물

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Cited By (31)

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Publication number Priority date Publication date Assignee Title
US9238687B2 (en) 2006-04-13 2016-01-19 Chugai Seiyaku Kabushiki Kaisha Method for recombinant production of a desired polypeptide using a mammalian cell co-expressing a taurine transporter polypeptide
US8796007B2 (en) 2007-03-15 2014-08-05 Chugai Seiyaku Kabushiki Kaisha Method for production of polypeptide
US9181540B2 (en) 2007-03-15 2015-11-10 Chugai Seiyaku Kabushiki Kaisha Method for production of polypeptide
US9284374B2 (en) 2007-08-07 2016-03-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
US9499618B2 (en) 2007-08-07 2016-11-22 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
US8697397B2 (en) 2007-08-07 2014-04-15 Chugai Seiyaku Kabushiki Kaisha Method of producing heterogeneous protein
US9802993B2 (en) 2007-10-15 2017-10-31 Chugai Seiyaku Kabushiki Kaisha Method for producing a cell for protein production by treating a cell overexpressing a taurine transporter with methotrexate
US9068212B2 (en) 2007-10-24 2015-06-30 Chugai Seiyaku Kabushiki Kaisha Method for producing a polypeptide using a cell that overexpresses a bicarbonate transporter
US8741601B2 (en) 2009-04-22 2014-06-03 Chugai Seiyaku Kabushiki Kaisha Method for producing a cell capable of high-yield production of heteroproteins
JP5715050B2 (ja) * 2009-04-22 2015-05-07 中外製薬株式会社 異種タンパク質を高生産する細胞の作製方法
WO2010123014A1 (ja) 2009-04-22 2010-10-28 中外製薬株式会社 異種タンパク質を高生産する細胞の作製方法
JPWO2012137683A1 (ja) * 2011-04-01 2014-07-28 中外製薬株式会社 組換えポリペプチドの製造方法
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