WO2008113342A2 - Procédé de production d'acides gras polyinstaurés - Google Patents

Procédé de production d'acides gras polyinstaurés Download PDF

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Publication number
WO2008113342A2
WO2008113342A2 PCT/DE2008/000492 DE2008000492W WO2008113342A2 WO 2008113342 A2 WO2008113342 A2 WO 2008113342A2 DE 2008000492 W DE2008000492 W DE 2008000492W WO 2008113342 A2 WO2008113342 A2 WO 2008113342A2
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WO
WIPO (PCT)
Prior art keywords
fatty acids
oil
moss
production
medium
Prior art date
Application number
PCT/DE2008/000492
Other languages
German (de)
English (en)
Other versions
WO2008113342A3 (fr
Inventor
Christian Uphoff
Original Assignee
Georg Fritzmeier Gmbh & Co. Kg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Georg Fritzmeier Gmbh & Co. Kg filed Critical Georg Fritzmeier Gmbh & Co. Kg
Publication of WO2008113342A2 publication Critical patent/WO2008113342A2/fr
Publication of WO2008113342A3 publication Critical patent/WO2008113342A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone

Definitions

  • the invention relates to a process for the production of polyunsaturated fatty acids.
  • Polyunsaturated fatty acids are proven to be important nutritional supplements that are suitable for reducing atherosclerosis and coronary heart disease as well as cardiac arrhythmias and sudden cardiac death. For primary prevention, it is therefore advised to use as many as possible fatty sea fish several times a week to increase the amount of unsaturated fatty acids in the body.
  • daily dietary supplements can be taken from concentrated fish oil, with the minimum requirement for omega-3 fatty acids being 0.1 g / day, according to recent studies, but for optimum use being 1 to 2 g / day.
  • oils from deep-sea fish are suitable as starting materials for such fish oil products. Since it can be expected that the supply and / or the demand for fish oil products will increase in the future due to the proven positive mode of action, the question of dwindling resources, especially if the known production channels are to cover this high demand, arises in this context concentrated fish oil is no longer sufficient. It is therefore necessary to look for ways to develop better concentrating methods or other omega-3 fatty acid sources.
  • the invention has for its object to provide an improved process for the production of unsaturated fatty acids, for example, omega-3 or omega-6 fatty acids available. This object is achieved by a method having the features of claim 1.
  • the production of said fatty acids can take place in different ways:
  • the concentration of said fatty acids can take place by means of a mixture of photosynthetically active and light-emitting microorganisms, as described in the patent applications DE 100 62 812 A1, DE 101 49 447 A1 or DE 103 51 996 A1 of the applicant. These microorganisms mixture is sold by the applicant under the name "reacre ®”. It showed surprising as that these microorganisms are also able to concentrate shares in polyunsaturated fatty acids of oils, such as fish oil or the like.
  • Microorganisms that incorporate the polyunsaturated fatty acids and / or convert them into their own cell constituents so that they are no longer available in the oil are relatively numerous. With a suitable choice of microorganisms, it is then possible to use the microorganisms with the stored and concentrated unsaturated fatty acids directly as a dietary supplement.
  • lactic acid bacteria can be added to the microorganism mixture according to the invention, so that the property of the lactic acid bacteria to absorb fatty acids can be exploited.
  • fatty acids can be enriched in and on bacterial cells and thus possibly also obtained in a purer form, as they were previously in fish oil. The corresponding bacteria, however, are not producers of said fatty acids. They are only used to store or store fatty acids.
  • fatty acid-rich media for example fish waste
  • EPA and 20% of DHA could be detected in certain strains of Lactobacillus acidophilus, Lactococcus lactis, Lactococcus xylosus and Streptococcus thermophilus. Enriched in this way Lactic acid bacteria could be used directly as a dietary supplement.
  • the supplementation is not limited to the sole enrichment of DHA and EPA 1 it is also possible to enrich other lipids such as glycolipids, aminolipids, sulfolipids, sphingolipids.
  • the method of the invention still holds in the context of the application a great potential for optimization, for example, in terms of the composition of fatty acid-enriching microorganisms open, so that the proportions of the corresponding fatty acids could certainly be increased.
  • the following list shows some candidates that can be used to enrich fatty acids and the proportions of EPA and DHA in the total amount of fatty acids.
  • Bacillus psychrophilus 2 1 Bacillus subtilis - -
  • the recombinant production of polyunsaturated fatty acids can be performed on other multicellular organisms, in particular mosses. Particularly advantageous here is the moss Physcomitrella patens, since it already has a high level of self-produced arachidonic acid and, although a not so high level of EPA.
  • the yield can be increased by additionally cloning the genes for a multienzyme complex for DHA and EPA production from prokaryotes into the moss.
  • E. coli EPA with a proportion of more than 20% of all fatty acids and DHA with 5%
  • the mosses also represent an expression system for eukaryotic genes, since they make posttranslational modifications - ie the attachment of different molecules to the finished protein - as it is typical in eukaryotic cells.
  • a eukaryotic complex protein produced in E. coli does not have such modifications and therefore exhibits less activity.
  • the fact that the foreign protein has a toxic effect on the cell can be counteracted, for example, by discharging it.
  • a very complex multienzyme complex of bacterial origin can thus be produced which, moreover, catalyzes the metabolic pathway in the intact eukaryotic cell, so that the products DHA and EPA end up.
  • the abovementioned mosses are arranged on a membrane and thereby docked to the moss dinoflaggelanten, wherein the fatty acids are withdrawn through the membrane. These are produced via the moss and dinoflaggelanten.
  • the multicellular organisms or the microorganism mixture can be charged with energy pulses. It is preferred if the growth is excited by exposure to laser pulses, for example, every minute a laser pulse is emitted.
  • a laser can be used, as it is used for fluorescence excitation of plant chlorophyll (see WO 2000 025 114 A1). Such a laser system is distributed by the applicant under the trade name "MiniVeg.” Alternatively, a so-called Greenlight laser be used. Such lasers are also used in surgery.
  • a nutrient solution can be added.
  • hops may also be used as a raw material for the production of unsaturated fatty acids.
  • a high proportion of unsaturated fatty acids already occurs in hops, especially in the hops seed, as shown in the following table:
  • the proportion can be optimized so that in or on the hops a high proportion of polyunsaturated fatty acids contained oils are incorporated or attached.
  • the fatty acids can then be separated by extraction from the hops.
  • the polyunsaturated fatty acids from the hop seeds with archaea, lactic acid bacteria or other microorganisms or microorganisms mixtures such as "reacre ®", extracted, enriched and / or isolated.
  • lactic acid bacteria they can store the polyunsaturated fatty acids of the hop seeds
  • the incorporation of polyunsaturated fatty acids has the advantage of counteracting the tendency to become slightly rancid.
  • the figure summarizes the different ways of concentrating / producing polyunsaturated fatty acids.
  • oils based on soya, oilseed rape or walnut, as well as linseed oil, pyrilla oil or simple olive oil can markedly increase the concentration of omega-3 fatty acids contained therein.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé de production d'acides gras polyinstaurés à partir d'un milieu les contenant, les acides gras étant concentrés par des micro-organismes ou par des organismes multicellulaires dans un milieu contenant les acides gras ou bien absorbés ou produits à partir de ce milieu.
PCT/DE2008/000492 2007-03-20 2008-03-20 Procédé de production d'acides gras polyinstaurés WO2008113342A2 (fr)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
DE102007013850 2007-03-20
DE102007013850.6 2007-03-20
DE102007014063 2007-03-21
DE102007014063.2 2007-03-21
DE102008015239A DE102008015239A1 (de) 2007-03-20 2008-03-20 Verfahren zur Produktion mehrfach ungesättigter Fettsäuren
DE102008015239.0 2008-03-20

Publications (2)

Publication Number Publication Date
WO2008113342A2 true WO2008113342A2 (fr) 2008-09-25
WO2008113342A3 WO2008113342A3 (fr) 2009-03-05

Family

ID=39713400

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2008/000492 WO2008113342A2 (fr) 2007-03-20 2008-03-20 Procédé de production d'acides gras polyinstaurés

Country Status (2)

Country Link
DE (1) DE102008015239A1 (fr)
WO (1) WO2008113342A2 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000025114A1 (fr) * 1998-10-28 2000-05-04 Deutsches Zentrum für Luft- und Raumfahrt e.V. Ensemble de detection de fluorescence pour la determination de parametres pertinents de vegetation
WO2002049971A1 (fr) * 2000-12-18 2002-06-27 Georg Fritzmeier Gmbh & Co. Composition microbiologique
DE10063387A1 (de) * 2000-12-19 2002-09-12 Basf Ag Neues Elongasegen und Verfahren zur Herstellung mehrfach ungestättigter Fettsäuren
EP1500706A1 (fr) * 2002-04-12 2005-01-26 Kabushiki Kaisha Yakult Honsha Procede de production d'acide gras conjugue et aliment /boisson obtenus grace a ce procede
WO2005103253A1 (fr) * 2004-04-22 2005-11-03 Commonwealth Scientific And Industrial Research Organisation Synthese d'acides gras polyinsatures a chaine longue par des cellules de recombinaison

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130242A (en) 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
DE20022664U1 (de) 2000-12-27 2002-01-03 Georg Fritzmeier GmbH & Co., 85655 Großhelfendorf Koditioniermittel zur Behandlung von Abwasser
DE10351996A1 (de) 2003-11-07 2005-06-09 Giuseppe Burgio Vorrichtung zum Zuschneiden von Wand- und Deckenbelegen (Tapeten)

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000025114A1 (fr) * 1998-10-28 2000-05-04 Deutsches Zentrum für Luft- und Raumfahrt e.V. Ensemble de detection de fluorescence pour la determination de parametres pertinents de vegetation
WO2002049971A1 (fr) * 2000-12-18 2002-06-27 Georg Fritzmeier Gmbh & Co. Composition microbiologique
DE10063387A1 (de) * 2000-12-19 2002-09-12 Basf Ag Neues Elongasegen und Verfahren zur Herstellung mehrfach ungestättigter Fettsäuren
EP1500706A1 (fr) * 2002-04-12 2005-01-26 Kabushiki Kaisha Yakult Honsha Procede de production d'acide gras conjugue et aliment /boisson obtenus grace a ce procede
WO2005103253A1 (fr) * 2004-04-22 2005-11-03 Commonwealth Scientific And Industrial Research Organisation Synthese d'acides gras polyinsatures a chaine longue par des cellules de recombinaison

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DATABASE FSTA [Online] INTERNATIONAL FOOD INFORMATION SERVICE (IFIS); DEMIREVA, Z.N. ET AL.: "Studies on the fatty acid composition of hop extract." XP002508311 Database accession no. 96-1-03-h0018 & JOURNAL OF THE INSTITUTE OF BREWING, Bd. 101, Nr. 6, 1995, Seiten 437-438, *
KANKAANPÄÄ, P. ET AL.: "Effects of Polyunsaturated Fatty Acids in Growth Medium on Lipid Composition and on Physicochemical Surface Properties of Lactobacilli" APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Bd. 70, Nr. 1, Januar 2004 (2004-01), Seiten 129-136, XP002500396 *

Also Published As

Publication number Publication date
DE102008015239A1 (de) 2008-09-25
WO2008113342A3 (fr) 2009-03-05

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