WO2008111530A1 - 脱細胞化軟組織の調製方法、移植片、及び培養部材 - Google Patents

脱細胞化軟組織の調製方法、移植片、及び培養部材 Download PDF

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Publication number
WO2008111530A1
WO2008111530A1 PCT/JP2008/054207 JP2008054207W WO2008111530A1 WO 2008111530 A1 WO2008111530 A1 WO 2008111530A1 JP 2008054207 W JP2008054207 W JP 2008054207W WO 2008111530 A1 WO2008111530 A1 WO 2008111530A1
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Prior art keywords
soft tissue
preparing
graft
culture material
decellularized soft
Prior art date
Application number
PCT/JP2008/054207
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English (en)
French (fr)
Inventor
Akio Kishida
Tsuyoshi Kimura
Hisatoshi Kobayashi
Toshiya Fujisato
Original Assignee
National University Corporation, Tokyo Medical And Dental University
The Japan Health Sciences Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National University Corporation, Tokyo Medical And Dental University, The Japan Health Sciences Foundation filed Critical National University Corporation, Tokyo Medical And Dental University
Priority to US12/530,510 priority Critical patent/US8486139B2/en
Priority to EP08721624.8A priority patent/EP2138181B1/en
Priority to JP2009504037A priority patent/JP5331960B2/ja
Publication of WO2008111530A1 publication Critical patent/WO2008111530A1/ja

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
    • A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Transplantation (AREA)
  • Biotechnology (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Vascular Medicine (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Materials For Medical Uses (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

　本発明の課題は、超高圧処理後の膨潤を抑制できる脱細胞化軟組織の調製方法等を提供することである。 　本発明に係る脱細胞化軟組織の調製方法は、単離された軟組織に媒体中で超高静水圧を印加することで、軟組織内の細胞を破壊する印加手順と、破壊された細胞を除去する除去手順と、を含む。媒体は、デキストラン等の水溶性多糖を含有する水溶液である。
PCT/JP2008/054207 2007-03-09 2008-03-07 脱細胞化軟組織の調製方法、移植片、及び培養部材 WO2008111530A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/530,510 US8486139B2 (en) 2007-03-09 2008-03-07 Method of preparing decellularized soft tissue, graft and culture material
EP08721624.8A EP2138181B1 (en) 2007-03-09 2008-03-07 Method of preparing decellularized soft tissue
JP2009504037A JP5331960B2 (ja) 2007-03-09 2008-03-07 脱細胞化軟組織の調製方法、移植片、及び培養部材

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007061025 2007-03-09
JP2007-061025 2007-03-09

Publications (1)

Publication Number Publication Date
WO2008111530A1 true WO2008111530A1 (ja) 2008-09-18

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PCT/JP2008/054207 WO2008111530A1 (ja) 2007-03-09 2008-03-07 脱細胞化軟組織の調製方法、移植片、及び培養部材

Country Status (4)

Country Link
US (1) US8486139B2 (ja)
EP (1) EP2138181B1 (ja)
JP (1) JP5331960B2 (ja)
WO (1) WO2008111530A1 (ja)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010227246A (ja) * 2009-03-26 2010-10-14 Tokyo Medical & Dental Univ 脱細胞化生体組織の調製方法
WO2011022369A3 (en) * 2009-08-18 2011-05-12 Lifecell Corporation Method for processing tissues
WO2013032009A1 (ja) 2011-09-02 2013-03-07 株式会社エフコム 脱細胞化組織製品の調製方法、及び脱細胞化組織製品を備える移植片
WO2014034759A1 (ja) * 2012-08-31 2014-03-06 国立大学法人京都大学 加圧処理を用いた組織の脱細胞化方法
WO2014181767A1 (ja) 2013-05-07 2014-11-13 国立大学法人 東京医科歯科大学 粒子状脱細胞化組織の製造方法
JP2015528340A (ja) * 2012-09-04 2015-09-28 ユニヴァーシティ オブ リーズＵｎｉｖｅｒｓｉｔｙ Ｏｆ Ｌｅｅｄｓ 複合骨移植片
KR20160025502A (ko) 2013-05-07 2016-03-08 잇빤 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 입자상 탈세포화 조직을 포함하는 하이브리드 겔
KR20170122178A (ko) 2015-02-27 2017-11-03 가부시키가이샤 아데카 탈세포화 조직
KR20170128385A (ko) 2015-03-12 2017-11-22 잇빤 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 탈세포화 조직을 사용한 유착 방지재 및 대용 생체막
WO2018221402A1 (ja) 2017-05-30 2018-12-06 哲哉 樋上 移植用脱細胞化材料の製造方法及び当該材料を含む生体適合性材料からなる移植片組成物

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US8628572B2 (en) * 2009-02-26 2014-01-14 Wake Forest University Health Sciences Corneal endothelial scaffolds and methods of use
JP5897002B2 (ja) 2010-07-07 2016-04-13 セルラー ダイナミクス インターナショナル， インコーポレイテッド プログラミングによる内皮細胞の産生
CN103908700B (zh) * 2013-01-06 2015-09-09 陕西佰傲再生医学有限公司 一种脱细胞角膜的制备方法
WO2015134618A1 (en) 2014-03-04 2015-09-11 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Method and apparatus for decellularization of tissue
US20190240003A1 (en) * 2016-09-12 2019-08-08 Gebauer-Klopotek Patent Verwaltungs-Ug Lenticules for intrastromal corneal implantation
CN107050515B (zh) * 2017-03-03 2020-01-21 北京博辉瑞进生物科技有限公司 一种角膜基质、制备方法与应用
CN111110917A (zh) * 2018-10-30 2020-05-08 上海微创心通医疗科技有限公司 一种制备脱细胞生物组织材料的方法
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Cited By (24)

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JP2010227246A (ja) * 2009-03-26 2010-10-14 Tokyo Medical & Dental Univ 脱細胞化生体組織の調製方法
WO2011022369A3 (en) * 2009-08-18 2011-05-12 Lifecell Corporation Method for processing tissues
JP2013502275A (ja) * 2009-08-18 2013-01-24 ライフセル コーポレーション 組織の処理方法
AU2010284365B2 (en) * 2009-08-18 2015-01-29 Lifecell Corporation Method for processing tissues
US9023273B2 (en) 2009-08-18 2015-05-05 Lifecell Corporation Method for processing tissues
CN107115567A (zh) * 2009-08-18 2017-09-01 生命细胞公司 用于处理组织的方法
EP3103461A1 (en) * 2009-08-18 2016-12-14 Lifecell Corporation Method for sterilising dermis
WO2013032009A1 (ja) 2011-09-02 2013-03-07 株式会社エフコム 脱細胞化組織製品の調製方法、及び脱細胞化組織製品を備える移植片
US9901601B2 (en) 2011-09-02 2018-02-27 Adeka Corporation Method for preparing decellularized tissue product, and graft provided with decellularized tissue product
WO2014034759A1 (ja) * 2012-08-31 2014-03-06 国立大学法人京都大学 加圧処理を用いた組織の脱細胞化方法
JP2015528340A (ja) * 2012-09-04 2015-09-28 ユニヴァーシティ オブ リーズＵｎｉｖｅｒｓｉｔｙ Ｏｆ Ｌｅｅｄｓ 複合骨移植片
US9901450B2 (en) 2012-09-04 2018-02-27 University Of Leeds Composite bone implants
JPWO2014181767A1 (ja) * 2013-05-07 2017-02-23 国立大学法人 東京医科歯科大学 粒子状脱細胞化組織の製造方法
KR20160025502A (ko) 2013-05-07 2016-03-08 잇빤 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 입자상 탈세포화 조직을 포함하는 하이브리드 겔
KR20160005712A (ko) 2013-05-07 2016-01-15 고쿠리츠 다이가쿠호우징 도쿄이카시카다이가쿠 입자상 탈세포화 조직의 제조방법
WO2014181767A1 (ja) 2013-05-07 2014-11-13 国立大学法人 東京医科歯科大学 粒子状脱細胞化組織の製造方法
JP2019030332A (ja) * 2013-05-07 2019-02-28 国立大学法人 東京医科歯科大学 粒子状脱細胞化組織の製造方法
EP3556406A1 (en) 2013-05-07 2019-10-23 National University Corporation Tokyo Medical and Dental University Method for producing particulate decellularized tissue
KR102240373B1 (ko) 2013-05-07 2021-04-13 고쿠리츠 다이가쿠호우징 도쿄이카시카다이가쿠 입자상 탈세포화 조직의 제조방법
KR20170122178A (ko) 2015-02-27 2017-11-03 가부시키가이샤 아데카 탈세포화 조직
KR20170128385A (ko) 2015-03-12 2017-11-22 잇빤 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 탈세포화 조직을 사용한 유착 방지재 및 대용 생체막
US11033661B2 (en) 2015-03-12 2021-06-15 Adeka Corporation Anti-adhesion material and substitute biomembrane using decellularized tissue
WO2018221402A1 (ja) 2017-05-30 2018-12-06 哲哉 樋上 移植用脱細胞化材料の製造方法及び当該材料を含む生体適合性材料からなる移植片組成物
KR20200016226A (ko) 2017-05-30 2020-02-14 가부시키가이샤 아데카 이식용 탈세포화 재료의 제조 방법 및 당해 재료를 포함하는 생체 적합성 재료로 이루어지는 이식편 조성물

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US20100145444A1 (en) 2010-06-10
US8486139B2 (en) 2013-07-16
JPWO2008111530A1 (ja) 2010-06-24
JP5331960B2 (ja) 2013-10-30
EP2138181A4 (en) 2012-11-28
EP2138181A1 (en) 2009-12-30
EP2138181B1 (en) 2016-05-04

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