WO2008105512A1 - チトクロームp-450遺伝子発現大腸菌およびそれらを用いた微生物変換方法 - Google Patents
チトクロームp-450遺伝子発現大腸菌およびそれらを用いた微生物変換方法 Download PDFInfo
- Publication number
- WO2008105512A1 WO2008105512A1 PCT/JP2008/053563 JP2008053563W WO2008105512A1 WO 2008105512 A1 WO2008105512 A1 WO 2008105512A1 JP 2008053563 W JP2008053563 W JP 2008053563W WO 2008105512 A1 WO2008105512 A1 WO 2008105512A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- derived
- coli cell
- cytochrome
- bioconversion
- same
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
Abstract
細菌由来のチトクロームP-450遺伝子を組み込んだ大腸菌を用いた微生物変換系においてみられる変換効率の低さを改善する手段の提供を目的とする。大腸菌に、アシネトバクター・エスピー(Acinetobacter sp.) OC4株由来の電子伝達系をコードする遺伝群aciBCを組み込むとともに、細菌由来のチトクロームP-450遺伝子の5'末端側に、アシネトバクター・エスピー(Acinetobacter sp.) OC4株由来のアルカン酸化性チトクロームP-450であるAciAのN末端48アミノ酸等をコードするポリヌクレオチドを付加した組換え大腸菌を作製し、これを微生物変換に使用することにより、疎水性または両親媒性の基質化合物を非常に効率よくその目的化合物に変換することができる。
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/449,876 US8460915B2 (en) | 2007-03-01 | 2008-02-28 | Escherichia coli expressing the cytochrome P-450 gene and a method for microbial conversion using them |
JP2009501307A JPWO2008105512A1 (ja) | 2007-03-01 | 2008-02-28 | チトクロームp−450遺伝子発現大腸菌およびそれらを用いた微生物変換方法 |
EP08721012.6A EP2130916B1 (en) | 2007-03-01 | 2008-02-28 | E. coli capable of expressing cytochrome p-450, and bioconversion method using the same |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007-050936 | 2007-03-01 | ||
JP2007050936 | 2007-03-01 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2008105512A1 true WO2008105512A1 (ja) | 2008-09-04 |
Family
ID=39721334
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2008/053563 WO2008105512A1 (ja) | 2007-03-01 | 2008-02-28 | チトクロームp-450遺伝子発現大腸菌およびそれらを用いた微生物変換方法 |
Country Status (4)
Country | Link |
---|---|
US (1) | US8460915B2 (ja) |
EP (1) | EP2130916B1 (ja) |
JP (1) | JPWO2008105512A1 (ja) |
WO (1) | WO2008105512A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2446668T3 (es) * | 2008-12-19 | 2014-03-10 | Ildong Pharmaceutical Co., Ltd. | Composición de tampón para catalizar la preparación de calcitriol o calcifediol y método para preparar calcitriol o calcifediol usando esta |
CN110923183A (zh) * | 2019-12-13 | 2020-03-27 | 江苏师范大学 | 产羊毛甾醇大肠杆菌菌株的构建方法 |
CN116590206A (zh) * | 2021-03-02 | 2023-08-15 | 天津科技大学 | 具有优良转运和反应活性的甾体c1,2脱氢反应工程菌株 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0767666A (ja) * | 1994-08-12 | 1995-03-14 | Sumitomo Chem Co Ltd | フェレドキシン及びフェレドキシン還元酵素を同時に発現させるキメラp450産生菌株 |
WO2003087381A1 (fr) * | 2002-04-12 | 2003-10-23 | Mercian Corporation | Systeme d'expression de cytochrome p-450 d'origine actinomycete dans des escherichia coli |
WO2006051729A1 (ja) * | 2004-11-10 | 2006-05-18 | Marine Biotechnology Institute Co., Ltd. | P450遺伝子の単離法 |
JP2007050936A (ja) | 2005-08-15 | 2007-03-01 | Apollo Giken:Kk | パーツフィーダ |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61272492A (ja) | 1985-05-25 | 1986-12-02 | Toshiba Corp | 多気筒形回転圧縮機 |
JPS6270686A (ja) | 1985-09-20 | 1987-04-01 | Sanyo Electric Co Ltd | 多気筒回転圧縮機 |
JP2904572B2 (ja) | 1990-10-31 | 1999-06-14 | 株式会社東芝 | 多気筒型回転圧縮機 |
JPH04241791A (ja) | 1991-01-10 | 1992-08-28 | Toshiba Corp | 多気筒型回転圧縮機 |
JP3414797B2 (ja) | 1993-08-12 | 2003-06-09 | 東芝キヤリア株式会社 | 多気筒形回転圧縮機 |
JP3408005B2 (ja) | 1995-01-30 | 2003-05-19 | 三洋電機株式会社 | 多気筒回転圧縮機 |
TW336270B (en) | 1997-01-17 | 1998-07-11 | Sanyo Electric Ltd | Compressor and air conditioner |
-
2008
- 2008-02-28 WO PCT/JP2008/053563 patent/WO2008105512A1/ja active Application Filing
- 2008-02-28 JP JP2009501307A patent/JPWO2008105512A1/ja active Pending
- 2008-02-28 US US12/449,876 patent/US8460915B2/en active Active
- 2008-02-28 EP EP08721012.6A patent/EP2130916B1/en not_active Not-in-force
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0767666A (ja) * | 1994-08-12 | 1995-03-14 | Sumitomo Chem Co Ltd | フェレドキシン及びフェレドキシン還元酵素を同時に発現させるキメラp450産生菌株 |
WO2003087381A1 (fr) * | 2002-04-12 | 2003-10-23 | Mercian Corporation | Systeme d'expression de cytochrome p-450 d'origine actinomycete dans des escherichia coli |
US20060234337A1 (en) | 2002-04-12 | 2006-10-19 | Akira Arisawa | Expression system for actinomycete-origin cytochrome p-450 in escherichia coli |
WO2006051729A1 (ja) * | 2004-11-10 | 2006-05-18 | Marine Biotechnology Institute Co., Ltd. | P450遺伝子の単離法 |
JP2007050936A (ja) | 2005-08-15 | 2007-03-01 | Apollo Giken:Kk | パーツフィーダ |
Non-Patent Citations (7)
Also Published As
Publication number | Publication date |
---|---|
EP2130916A1 (en) | 2009-12-09 |
JPWO2008105512A1 (ja) | 2010-06-03 |
US8460915B2 (en) | 2013-06-11 |
US20100285565A1 (en) | 2010-11-11 |
EP2130916A4 (en) | 2010-05-26 |
EP2130916B1 (en) | 2015-09-02 |
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