CN107354138A - 一种单氧化酶及其转化植物甾醇的方法 - Google Patents
一种单氧化酶及其转化植物甾醇的方法 Download PDFInfo
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- CN107354138A CN107354138A CN201710564844.2A CN201710564844A CN107354138A CN 107354138 A CN107354138 A CN 107354138A CN 201710564844 A CN201710564844 A CN 201710564844A CN 107354138 A CN107354138 A CN 107354138A
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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CN108913643A (zh) * | 2018-08-01 | 2018-11-30 | 天津科技大学 | 一种提高分枝杆菌辅酶再生同时促进雄烯二酮生产的方法 |
CN115521964A (zh) * | 2022-09-19 | 2022-12-27 | 湖北共同生物科技有限公司 | 一种甾体激素药物中间体的制备方法 |
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CN106282080A (zh) * | 2016-08-10 | 2017-01-04 | 江南大学 | 一种新金色分枝杆菌来源的甾酮c27‑单加氧酶及其应用 |
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CN106282080A (zh) * | 2016-08-10 | 2017-01-04 | 江南大学 | 一种新金色分枝杆菌来源的甾酮c27‑单加氧酶及其应用 |
Non-Patent Citations (2)
Title |
---|
RODRIGUEZ-GARCIA,A. 等: "Mycobacterium sp. NRRL B-3805, complete genome", 《GENBANK DATABASE》 * |
吕陈峰 等: "利用胆固醇氧化酶转化胆固醇制备胆甾-4-烯-3-酮", 《无锡轻工大学学报》 * |
Cited By (3)
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CN108913643A (zh) * | 2018-08-01 | 2018-11-30 | 天津科技大学 | 一种提高分枝杆菌辅酶再生同时促进雄烯二酮生产的方法 |
CN115521964A (zh) * | 2022-09-19 | 2022-12-27 | 湖北共同生物科技有限公司 | 一种甾体激素药物中间体的制备方法 |
CN115521964B (zh) * | 2022-09-19 | 2024-04-12 | 湖北共同生物科技有限公司 | 一种甾体激素药物中间体的制备方法 |
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