Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/02—Burettes; Pipettes
  • B01L3/0275—Interchangeable or disposable dispensing tips
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/06—Fluid handling related problems
  • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/04—Closures and closing means
  • B01L2300/041—Connecting closures to device or container
  • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/06—Auxiliary integrated devices, integrated components
  • B01L2300/0681—Filter
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/06—Auxiliary integrated devices, integrated components
  • B01L2300/069—Absorbents; Gels to retain a fluid
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
  • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
  • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
  • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
  • Y10T436/00—Chemistry: analytical and immunological testing
  • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
  • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
  • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Definitions

• the present invention relates to an extraction and/or sample collection device used during sample preparation for chemical analysis Background
• the present invention is a pipette tip device for the extraction of liquid, semi-solid or solid solutions to be chemically analyzed and the methods for their use
• the pipette tip extraction device contains a screen or filter at its lower narrow end to contain solid particulate matter and a barrier at its upper wide end This barrier may be referred to as either a frit, an adaptor, a cap or a fitting and may be used interchangeably
• the upper frit is to be made of material that permits liquid solutions to flow through it, and the upper frit may be optional and replaced with other fittings at the top of the DPX tip such as a removable cap, an adaptor, a luer lock fitting, a 2- or 3-way (or multiple port) valve fitting, an o- ⁇ ng fitting, or septum cap
• the adaptor may also be designed to facilitate the movement of the pipette tip on robotic instrumentation By incorporating grooves on the outside of the adaptor, a robotic g ⁇ pper can pick up the tip and move it to various locations such as sample tubes and vials
• the inside of the adaptor is designed to make an air tight seal with a syringe needle so that liquid solvents and gases can be aspirated and dispensed with relatively high pressures
• the adaptor may contain a thin film to provide a seal to contain material inside of the tip prior to sample processing
• the pipette tip extraction device may contain solid-phase sorbent
• the pipette extraction tip is an improved disposable pipette extraction (DPX) tip, with the ability to introduce solvent and sample solutions to its top wide opening end
• the sorbent may be small particle size such as 5-20 microns in diameter, providing unsurpassed extraction efficiency The use of such small particle size requires high pressure (HP), and these HP-DPX tips therefore require the use of
• DPX-LCprep DPX-LCprep
• the pipette extraction tip may also serve as a sample collection container or tip (SC-Tip) in which samples can be delivered to the top of the tip
• SC-Tip sample collection container
• the pipette extraction tip can be used in the field for direct collection of samples, including solid samples
• the screen of the SC-Tip is used as a filtration medium to remove and filter particulate matter that may be present following the extraction process This represents the only application of pipette extraction devices that may be used for directly processing solid samples
• a new method for DPX extraction using liquid-liquid-sohd-phase extraction is also disclosed This unique method of extraction will greatly extend analyte extractions and applications
• Figure 1 shows a cross-section schematic diagram of a HP-DPX tip
• Figure 2 shows schematic diagrams of cross-sections of several caps or adaptors or fittings that can be used with a syringe device for connection to the top of the
• Figure 3 shows a schematic diagram of a preferred embodiment of the pipette tip device
• FIG. 4 shows chromatograms (GC/MS) of DPX extracts of tetrahydrocannabinol (THC) and carboxy-THC (COOH-THC)
• Figure 5 shows a schematic diagram demonstrating the steps involved in
• FIG. 6 shows a schematic diagram of the tandem use of HP-DPX with
• Figure 7 shows GC/MS chromatograms of some common pesticides extracted from lettuce by DPX (B) and HP-DPX (A)
• Figure 8 shows a chromatogram (GC/MS) of basic drugs extracted from urine using DPX-CX
• Figure 9 shows an HPLC chromatogram (with fluorescence detection) recorded following DPX-IA extraction of 20 ppb aflatoxin Bi (peak at 6 4 min) from corn meal
• Figure 10 shows chromatograms (GC/MS) of blank stomach contents (feed) extracted by DPX with (A) and without (B) a clean up step by DPX-CU
• Figure 1 1 shows a sample collection tip (SC-Tip) demonstrating the ability of the pipette extraction tips with adaptors to be used for field collection of samples as well as for sample processing Detailed Description of the Invention
• a major improvement to DPX is the development of high performance SPE by using small particle size sorbent material
• These DPX tips are referred to as high performance DPX, or HP-DPX
• the small particle size materials range up to 200 microns in diameter, preferably up to 100 microns, with a more preferred range of 5-40 microns and most preferred range of 5-20 microns Use of these materials create higher back pressures and therefore require relatively higher pressures to perform extractions HP-DPX tips can therefore not be simply press fitted onto standard pipettes to accomplish these extractions Greater amounts of force then used with conventional pipettes for standard tips are required to move solutions in and out of the HP-DPX tips
• a 1 mL volume pipette (or syringe) can not be readily used with a 1 mL HP-DPX tip, instead, a 5 mL volume syringe or pipette device is required
• a 10 or even 25 mL syringe device is
• HP-DPX tips Another feature that makes these HP-DPX tips unique is that the mixing of the solutions with many of these materials creates a "gel", ie, the solution is homogenous and therefore provides unsurpassed extraction efficiencies and rapid equilibrations
• the unique mixing of HP-DPX makes these particular materials ideally suited for DPX technology
• One way of readily accomplishing this mixing is by aspirating air or another gas into the DPX tips, and this air flow causes the formation of small bubbles that cause a perturbation of the sample solution resulting in thorough mixing This method of mixing is readily amenable to robotic liquid handlers
• FIG 1 a schematic of the HP-DPX tip is shown
• the cross-section schematic diagram of an HP-DPX tip indicates an optional second frit (upper barrier, 22) composed of a liquid permeable membrane and loosely contained small particle size (such as 5 to 40 microns in diameter) sorbent material or other functionahzed sorbent particles ( 18)
• the void ( 10) indicates the mixing chamber of DPX, and the bottom ( 12) of the tip contains an opening (14) with a proximate screen or frit (24) that contains very small openings or pores (such as 2 or 5 or 10 microns in diameter)
• the screen (24) may be composed of stainless steel, porous polymeric material, porous glass, porous ceramic, or other similar materials
• the major improvements to this device is that different sorbent materials are disclosed and the upper frit (barrier) is made to be liquid permeable and is optional Various sorbent materials include styrene divinyl benzene, sdvb (5-40 microns in diameter
• a septum (A) or luer lock fitting adaptor (B) is shown in this case, a syringe needle or syringe can be used to pierce the septum or connect directly to the adaptor, respectively
• an adaptor can also be used that forms a friction seal with a syringe needle
• an o- ⁇ ng seal can be used to provide a seal for a syringe needle, a valve, luer lock fitting or any
• COOH-THC are shown following DPX extraction using elution solvent from the bottom (B) and elution from the top (A) (Note all chromatograms in this application show intensity counts vs time )
• the upper barrier was made of a porous polymeric material that allows for liquid solutions to pass through without interferences
• the peak representing COOH-THC is much greater in intensity with elution from the top
• the necessity for adding elution to the top is more noticeable with the use of HP-DPX tips, where the higher surface area causes retention of analyte when elution solvent is being passed back through the sorbent when eluting from the bottom, resulting in lower recoveries
• FIG. 6 a schematic diagram of the tandem use of HP-DPX with DPX-

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• Health & Medical Sciences (AREA)
• Clinical Laboratory Science (AREA)
• Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Sampling And Sample Adjustment (AREA)
• Automatic Analysis And Handling Materials Therefor (AREA)

Priority Applications (3)

Applications Claiming Priority (4)

Publications (1)

Family

ID=39710492

Family Applications (1)

Country Status (4)

Cited By (10)

Families Citing this family (28)

Citations (6)

Family Cites Families (35)

• 2008
  • 2008-02-21 EP EP08730397.0A patent/EP2125224A4/en active Pending
  • 2008-02-21 US US12/525,546 patent/US8715593B2/en not_active Expired - Fee Related
  • 2008-02-21 JP JP2009551010A patent/JP2010529416A/ja active Pending
  • 2008-02-21 WO PCT/US2008/054584 patent/WO2008103828A1/en not_active Ceased
• 2012
  • 2012-09-24 JP JP2012209306A patent/JP2013047680A/ja active Pending

Patent Citations (6)

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