WO2008102421A1 - 組換え微生物 - Google Patents

組換え微生物 Download PDF

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Publication number
WO2008102421A1
WO2008102421A1 PCT/JP2007/001481 JP2007001481W WO2008102421A1 WO 2008102421 A1 WO2008102421 A1 WO 2008102421A1 JP 2007001481 W JP2007001481 W JP 2007001481W WO 2008102421 A1 WO2008102421 A1 WO 2008102421A1
Authority
WO
WIPO (PCT)
Prior art keywords
gene
microorganism
regulatory region
prsa
transcriptional initiation
Prior art date
Application number
PCT/JP2007/001481
Other languages
English (en)
French (fr)
Inventor
Keiji Endo
Katsutoshi Ara
Original Assignee
Kao Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kao Corporation filed Critical Kao Corporation
Priority to EP07859666.5A priority Critical patent/EP2133416B1/en
Priority to DK07859666.5T priority patent/DK2133416T3/da
Priority to US12/528,039 priority patent/US8293516B2/en
Publication of WO2008102421A1 publication Critical patent/WO2008102421A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　タンパク質又はポリペプチドの生産性を向上させた組換え微生物及び当該組換え微生物を用いるタンパク質又はポリペプチドの製造方法の提供。 　微生物において機能を有する転写開始制御領域若しくは転写開始制御領域とリボソーム結合部位を、枯草菌<>prsA</>遺伝子又は当該遺伝子に相当する遺伝子のゲノム上における上流に導入してなるか、或いは微生物において機能を有する転写開始制御領域若しくは転写開始制御領域とリボソーム結合部位を枯草菌<>ｐｒｓＡ</>遺伝子又は当該遺伝子に相当する遺伝子の上流に連結した遺伝子断片を導入し、且つ<>abrB</>遺伝子、<>dltA</>遺伝子、<>dltB</>遺伝子、<>dltC</>遺伝子、<>dltD</>遺伝子、<>dltE</>遺伝子及び当該遺伝子に相当する遺伝子から選ばれる１以上の遺伝子を欠失又は不活性化してなる微生物株に、目的のタンパク質又はポリペプチドをコードする遺伝子を導入した組換え微生物。
PCT/JP2007/001481 2007-02-22 2007-12-27 組換え微生物 WO2008102421A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP07859666.5A EP2133416B1 (en) 2007-02-22 2007-12-27 Recombinant microorganism
DK07859666.5T DK2133416T3 (da) 2007-02-22 2007-12-27 Rekombinant mikroorganisme
US12/528,039 US8293516B2 (en) 2007-02-22 2007-12-27 Recombinant microorganism

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-042561 2007-02-22
JP2007042561A JP5226958B2 (ja) 2007-02-22 2007-02-22 組換え微生物

Publications (1)

Publication Number Publication Date
WO2008102421A1 true WO2008102421A1 (ja) 2008-08-28

Family

ID=39709706

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/001481 WO2008102421A1 (ja) 2007-02-22 2007-12-27 組換え微生物

Country Status (5)

Country Link
US (1) US8293516B2 (ja)
EP (1) EP2133416B1 (ja)
JP (1) JP5226958B2 (ja)
DK (1) DK2133416T3 (ja)
WO (1) WO2008102421A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108587996A (zh) * 2018-05-07 2018-09-28 湖北大学 高产聚γ-谷氨酸的工程菌及其构建方法与应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5361428B2 (ja) * 2009-02-06 2013-12-04 花王株式会社 枯草菌変異株
CN102174558B (zh) * 2011-03-24 2013-04-10 南京农业大学 通过敲除abrB基因提高枯草芽孢杆菌抗菌肽产量的方法
CN109022318B (zh) * 2018-08-15 2021-08-20 龙海市农丰源肥料有限公司 枯草芽孢杆菌l1及其在降解林可霉素菌渣中残留林可霉素的应用
WO2021146411A1 (en) * 2020-01-15 2021-07-22 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus licheniformis
WO2023091878A1 (en) 2021-11-16 2023-05-25 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
WO2023104846A1 (en) * 2021-12-10 2023-06-15 Novozymes A/S Improved protein production in recombinant bacteria
CN114875057A (zh) * 2022-06-14 2022-08-09 中农华威生物制药(湖北)有限公司 一种可高效表达饲用低温酸性α-淀粉酶的枯草芽孢杆菌的构建方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04190793A (ja) 1990-11-26 1992-07-09 Kao Corp セルラーゼ遺伝子を含む組換えプラスミド及び組換え微生物によるセルラーゼの製造方法
JP2000184882A (ja) 1998-12-21 2000-07-04 Kao Corp 新規アミラーゼ
JP2000210081A (ja) 1999-01-21 2000-08-02 Kao Corp 耐熱性アルカリセルラ―ゼ遺伝子
JP2006296242A (ja) * 2005-04-18 2006-11-02 Japan Agengy For Marine-Earth Science & Technology 宿主微生物

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1080307C (zh) * 1993-02-26 2002-03-06 芬兰国家公众健康机构 促进革兰氏阳性细菌中产生商业重要的外蛋白的方法和系统
GB9618107D0 (en) * 1996-08-30 1996-10-09 Secr Defence Vaccine production
US6403355B1 (en) * 1998-12-21 2002-06-11 Kao Corporation Amylases
CN100439492C (zh) * 2001-05-29 2008-12-03 花王株式会社 宿主微生物
JP4336082B2 (ja) * 2001-05-29 2009-09-30 花王株式会社 宿主微生物
JP4388272B2 (ja) * 2002-11-27 2009-12-24 花王株式会社 宿主微生物
JP2005137308A (ja) * 2003-11-07 2005-06-02 Kao Corp 組換え微生物
JP4509743B2 (ja) * 2003-11-07 2010-07-21 花王株式会社 組換え微生物
JP4336184B2 (ja) * 2003-11-07 2009-09-30 花王株式会社 組換え微生物
JP4820101B2 (ja) * 2004-03-05 2011-11-24 花王株式会社 変異バチルス属細菌
JP4496000B2 (ja) * 2004-04-07 2010-07-07 花王株式会社 宿主微生物

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH04190793A (ja) 1990-11-26 1992-07-09 Kao Corp セルラーゼ遺伝子を含む組換えプラスミド及び組換え微生物によるセルラーゼの製造方法
JP2000184882A (ja) 1998-12-21 2000-07-04 Kao Corp 新規アミラーゼ
JP2000210081A (ja) 1999-01-21 2000-08-02 Kao Corp 耐熱性アルカリセルラ―ゼ遺伝子
JP2006296242A (ja) * 2005-04-18 2006-11-02 Japan Agengy For Marine-Earth Science & Technology 宿主微生物

Non-Patent Citations (27)

* Cited by examiner, † Cited by third party
Title
"PCR Protocols. Current Methods and Applications", 1993, HUMANA PRESS, pages: 251
APPL. ENVIRON. MICROBIOL., vol. 68, 2002, pages 227
BAILLIE L. ET AL.: "THE EXPRESSION OF THE PROTECTIVE ANTIGEN OF BACILLUS ANTHRACIS IN BACILLUS SUBTILIS", J. APPL. MICROBIOL., vol. 84, no. 5, 1 May 1998 (1998-05-01), pages 741 - 746, XP009005078 *
BIOCHEM. J., vol. 280, 1991, pages 309
CELL MOL. LIFE SCI., vol. 59, 2002, pages 392
EUR. J. BIOCHEM., vol. 268, 2001, pages 3974
FEMS MICROBIOL. LETT., vol. 55, 1990, pages 135
GENE, vol. 77, 1989, pages 61
J. BACTERIOL., vol. 93, 1967, pages 1925
J. BIOL. CHEM., vol. 270, 1995, pages 15598
JAPAN FUNCTIONAL ANALYSIS NETWORK FOR BACILLUS SUBTILIS, 10 March 2004 (2004-03-10), Retrieved from the Internet <URL:http://bacillus.genome.ad.jp>
JOSEPH SAMBROOK; DAVID W. RUSSELL.: "Molecular Cloning - A LABORATORY MANUAL", COLD SPRING HARBOR LABORATORY PRESS
MICROBIOLOGY, vol. 145, 1999, pages 3409
MICROBIOLOGY., vol. 144, 1998, pages 3097
MOL. GEN. GENET., vol. 168, 1979, pages 111
MOL. GEN. GENET., vol. 209, 1987, pages 335
MOL. GEN. GENET., vol. 223, 1990, pages 268
MOL. MICROBIOL., vol. 7, 1993, pages 337
MOL. MICROBIOL., vol. 8, 1993, pages 727
NATURE, vol. 390, 1997, pages 249
NATURE, vol. 390, 1997, pages 249 - 256
PLASMID, vol. 18, 1987, pages 8
PROC. NATL. ACAD. SCI. USA, vol. 71, 1974, pages 1342
SCIENCE, vol. 227, 1985, pages 1435
SCIENCE, vol. 277, 1997, pages 1453
See also references of EP2133416A4 *
VITIKAINEN M. ET AL., J. APPL. MICROBIOL., vol. 99, no. 2, 2005, pages 363 - 375, XP008116658 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108587996A (zh) * 2018-05-07 2018-09-28 湖北大学 高产聚γ-谷氨酸的工程菌及其构建方法与应用
CN108587996B (zh) * 2018-05-07 2021-06-01 湖北大学 高产聚γ-谷氨酸的工程菌及其构建方法与应用

Also Published As

Publication number Publication date
EP2133416A1 (en) 2009-12-16
EP2133416B1 (en) 2014-09-10
US20100255534A1 (en) 2010-10-07
EP2133416A4 (en) 2010-11-10
JP2008200004A (ja) 2008-09-04
DK2133416T3 (da) 2014-11-10
US8293516B2 (en) 2012-10-23
JP5226958B2 (ja) 2013-07-03

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