WO2007097119A1 - SUPPORT DESTINE A LA CAPTURE D'ARNm ET PROCEDE DE PURIFICATION DE L'ARNm - Google Patents

SUPPORT DESTINE A LA CAPTURE D'ARNm ET PROCEDE DE PURIFICATION DE L'ARNm Download PDF

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Publication number
WO2007097119A1
WO2007097119A1 PCT/JP2007/000104 JP2007000104W WO2007097119A1 WO 2007097119 A1 WO2007097119 A1 WO 2007097119A1 JP 2007000104 W JP2007000104 W JP 2007000104W WO 2007097119 A1 WO2007097119 A1 WO 2007097119A1
Authority
WO
WIPO (PCT)
Prior art keywords
mrna
group
carrier
oligo
solution
Prior art date
Application number
PCT/JP2007/000104
Other languages
English (en)
Japanese (ja)
Inventor
Kanehisa Yokoyama
Kenji Kinoshita
Kentaro Fujimoto
Toru Yakabe
Shin Saito
Original Assignee
Sumitomo Bakelite Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Bakelite Co., Ltd. filed Critical Sumitomo Bakelite Co., Ltd.
Priority to JP2008501634A priority Critical patent/JP5262708B2/ja
Publication of WO2007097119A1 publication Critical patent/WO2007097119A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Definitions

  • mRNA When the number of bases of the oligo d T chain is in the range of 5 to 50, mRNA can be stably captured, and more preferably 15 to 35.
  • a polypropylene sampling tube (Fukae Kasei volume 1.5 ml) was hydrophilized by low-temperature oxygen plasma treatment.
  • a solution prepared by dissolving monoaminopropyl triethoxysilane as an aminoalkyl silane in methanol at a concentration of 5% by volume is prepared as an amino group introduction treatment solution, and this solution is dispensed into a tube for 2 hours. After standing, remove the solution by suction, and add ultrapure water. After dispensing and leaving, ultrapure water was removed by suction and dried.

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un porteur destiné à la capture d'un ARNm, ledit porteur comprenant un support insoluble et une substance polymère placée sur la surface du support. La substance polymère comprend : une première unité ayant un groupe dérivé d'un ester de phosphate qui constitue la partie hydrophile d'un phospholipide ; et une seconde unité ayant un groupe dérivé de l'acide carboxylique dans lequel un substituant preneur d'électron est lié à un groupe carbonyle. Une chaîne d'ADN contenant une séquence oligo-dT est liée au niveau de l'extrémité 5' de la séquence oligo-dT à tous les groupes dérivés de l'acide carboxylique ou à certains de ces groupes.
PCT/JP2007/000104 2006-02-21 2007-02-20 SUPPORT DESTINE A LA CAPTURE D'ARNm ET PROCEDE DE PURIFICATION DE L'ARNm WO2007097119A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2008501634A JP5262708B2 (ja) 2006-02-21 2007-02-20 ｍＲＮＡ捕捉用担体及びｍＲＮＡの精製方法

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2006-043351 2006-02-21
JP2006043351 2006-02-21
JP2006-302005 2006-11-07
JP2006302005 2006-11-07

Publications (1)

Publication Number Publication Date
WO2007097119A1 true WO2007097119A1 (fr) 2007-08-30

Family

ID=38437166

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2007/000104 WO2007097119A1 (fr) 2006-02-21 2007-02-20 SUPPORT DESTINE A LA CAPTURE D'ARNm ET PROCEDE DE PURIFICATION DE L'ARNm

Country Status (2)

Country Link
JP (1) JP5262708B2 (fr)
WO (1) WO2007097119A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009074056A (ja) * 2007-08-28 2009-04-09 Asahi Kasei Chemicals Corp 親水性ポリオレフィン焼結体

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001299344A (ja) * 2000-04-18 2001-10-30 Toyobo Co Ltd 純度の高いポリａ＋ｒｎａの精製方法
WO2005029095A1 (fr) * 2003-09-19 2005-03-31 Sumitomo Bakelite Co., Ltd. Puce a adn
JP2006084393A (ja) * 2004-09-17 2006-03-30 Sumitomo Bakelite Co Ltd バイオチップ
WO2006062009A1 (fr) * 2004-12-09 2006-06-15 Sumitomo Bakelite Co., Ltd. Procede d’elongation de la chaine d’adn, procede d’amplification de la chaine d’adn et puce a adn pour elongation de la chaine d’adn
WO2006123647A1 (fr) * 2005-05-17 2006-11-23 Sumitomo Bakelite Co., Ltd. Procede de detection de gene

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001299344A (ja) * 2000-04-18 2001-10-30 Toyobo Co Ltd 純度の高いポリａ＋ｒｎａの精製方法
WO2005029095A1 (fr) * 2003-09-19 2005-03-31 Sumitomo Bakelite Co., Ltd. Puce a adn
JP2006084393A (ja) * 2004-09-17 2006-03-30 Sumitomo Bakelite Co Ltd バイオチップ
WO2006062009A1 (fr) * 2004-12-09 2006-06-15 Sumitomo Bakelite Co., Ltd. Procede d’elongation de la chaine d’adn, procede d’amplification de la chaine d’adn et puce a adn pour elongation de la chaine d’adn
WO2006123647A1 (fr) * 2005-05-17 2006-11-23 Sumitomo Bakelite Co., Ltd. Procede de detection de gene

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009074056A (ja) * 2007-08-28 2009-04-09 Asahi Kasei Chemicals Corp 親水性ポリオレフィン焼結体

Also Published As

Publication number Publication date
JPWO2007097119A1 (ja) 2009-07-09
JP5262708B2 (ja) 2013-08-14

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