WO2006107864A1 - Screening using polarization anisotropy in fret emissions - Google Patents

Screening using polarization anisotropy in fret emissions Download PDF

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Publication number
WO2006107864A1
WO2006107864A1 PCT/US2006/012292 US2006012292W WO2006107864A1 WO 2006107864 A1 WO2006107864 A1 WO 2006107864A1 US 2006012292 W US2006012292 W US 2006012292W WO 2006107864 A1 WO2006107864 A1 WO 2006107864A1
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WO
WIPO (PCT)
Prior art keywords
anisotropy
fluorophore
fret
donor
measured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/012292
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English (en)
French (fr)
Inventor
Steven C. Miller
Paul B. Comita
Christopher B. Shumate
Evan F. Cromwell
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Blueshift Biotechnologies Inc
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Blueshift Biotechnologies Inc
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Publication date
Application filed by Blueshift Biotechnologies Inc filed Critical Blueshift Biotechnologies Inc
Priority to JP2008505419A priority Critical patent/JP4921458B2/ja
Priority to EP06758259A priority patent/EP1866443A4/en
Publication of WO2006107864A1 publication Critical patent/WO2006107864A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Definitions

  • Fluorescence generated from a FRET acceptor fluorophores is depolarized from the FRET process, and generally has relatively lower anisotropy than the fluorescence generated directly from donor fluorophores.
  • anisotropy can be used as a measure of FRET, and, consequently, the associated proximity of donor and acceptor fluorophores.
  • the use of this technique in homo-FRET, or FRET between like fluorophores has been described in "Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)" (Lidke, D.
  • FIG. 9 shows a bar diagram illustrating the average yellow/blue intensity ratios for the calibration beads of FIG. 7.
  • FIG. 10 shows a bar diagram illustrating the average anisotropy for CFP/YFP calibration beads of FIG. 7.
  • a dye fluorophore
  • a dye also with a high intrinsic anisotropy, that functions as a donor in a FRET pair, however, has essentially the same anisotropy when it is a donor in a FRET pair and when it is not interacting as a donor in a FRET pair.
  • the invention is not limited to beads and live cells, but any type of objects or combinations of objects suitable for FRET analysis can be used.
  • objects or combinations thereof include microbeads, spots, spot on spot, spot on a slide combined with a bead, samples or objects confined in capillary tubes or microfluidic channels, and so on.
  • the central idea is to simultaneously measure anisotropy for two different wavelength regions for a sample confined in a well- defined target region, and to use one of the measured anisotropics as an internal reference for detecting and/or quantifying a change of the other measured anisotropy for the sample in the target region, thereby allowing for detection of the presence, absence and/or quantity of FRET in the target region.

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/US2006/012292 2005-04-04 2006-04-03 Screening using polarization anisotropy in fret emissions Ceased WO2006107864A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2008505419A JP4921458B2 (ja) 2005-04-04 2006-04-03 Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法
EP06758259A EP1866443A4 (en) 2005-04-04 2006-04-03 SCREENING USING POLARIZATION ANISOTROPY FOR FRET EMISSIONS

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US66842805P 2005-04-04 2005-04-04
US60/668,428 2005-04-04
US67884205P 2005-05-06 2005-05-06
US60/678,842 2005-05-06

Publications (1)

Publication Number Publication Date
WO2006107864A1 true WO2006107864A1 (en) 2006-10-12

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PCT/US2006/012292 Ceased WO2006107864A1 (en) 2005-04-04 2006-04-03 Screening using polarization anisotropy in fret emissions

Country Status (5)

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US (2) US20060234279A1 (enExample)
EP (1) EP1866443A4 (enExample)
JP (1) JP4921458B2 (enExample)
TW (1) TW200704778A (enExample)
WO (1) WO2006107864A1 (enExample)

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JP2008225013A (ja) * 2007-03-12 2008-09-25 Olympus Corp レーザー走査型顕微鏡
WO2011012646A2 (en) 2009-07-28 2011-02-03 F. Hoffmann-La Roche Ag Non-invasive in vivo optical imaging method
WO2012119999A1 (en) 2011-03-07 2012-09-13 F. Hoffmann-La Roche Ag Means and methods for in vivo testing of therapeutic antibodies
WO2012120004A1 (en) 2011-03-07 2012-09-13 F. Hoffmann-La Roche Ag In vivo selection of therapeutically active antibodies
CN103582817A (zh) * 2011-04-20 2014-02-12 韩国科学技术院 在细胞环境中于单分子水平上分析蛋白-蛋白相互作用的方法，和测定在细胞溶质中激活的蛋白密度的方法
WO2016116291A1 (de) * 2015-01-23 2016-07-28 Bayer Cropscience Ag Verfahren und vorrichtung zur ermittlung der wirkung von wirkstoffen an nematoden und anderen organismen in wässrigen tests
CN109509509A (zh) * 2018-09-29 2019-03-22 江西理工大学 基于动态加权蛋白质相互作用网络的蛋白质复合物挖掘方法

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WO2006107864A1 (en) * 2005-04-04 2006-10-12 Blueshift Biotechnologies, Inc. Screening using polarization anisotropy in fret emissions
ES2462759T3 (es) 2008-10-01 2014-05-26 Sherwin Hua Sistema para estabilización de tornillo pedicular guiado por alambre de vertebras de la columna
US8568306B2 (en) 2009-04-03 2013-10-29 Mitchell A Hardenbrook Surgical retractor system
MX2012011091A (es) 2010-03-26 2013-03-07 Echostar Technologies Llc Receptor de television de entrada multiple.
CA2794282C (en) 2010-03-30 2018-11-20 Sherwin Hua Systems and methods for pedicle screw stabilization of spinal vertebrae
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
WO2012154858A1 (en) * 2011-05-09 2012-11-15 Whitehead Institute For Biomedical Research Chaperone interaction assays and uses thereof
JP5877741B2 (ja) * 2012-03-16 2016-03-08 株式会社日立製作所 細胞数モニタリング方法
WO2014059281A1 (en) * 2012-10-11 2014-04-17 The Regents Of The University Of Michigan Fluorescence polarization assay for detecting high molecular weight molecules in biological fluids
CN102967554B (zh) * 2012-10-29 2016-12-21 广东工业大学 一种双通道、单光路结构的荧光各向异性显微成像装置及方法
ES2829612T3 (es) * 2013-10-25 2021-06-01 Biomadison Inc Ensayo enzimático con fluoróforos duplicados
US11160580B2 (en) 2019-04-24 2021-11-02 Spine23 Inc. Systems and methods for pedicle screw stabilization of spinal vertebrae
CN115551426B (zh) 2019-11-27 2025-11-28 斯拜因23公司 治疗脊柱侧弯的系统、装置和方法
US11782001B2 (en) 2020-12-04 2023-10-10 Attolight AG Dislocation type and density discrimination in semiconductor materials using cathodoluminescence measurements
TWI808554B (zh) * 2020-12-04 2023-07-11 亞光股份有限公司 使用陰極發光測量判別半導體材料中的位錯類型和密度的裝置與方法
EP4337119B1 (en) 2021-05-12 2025-12-10 Spine23 Inc. Systems for pedicle screw stabilization of spinal vertebrae

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JP2008225013A (ja) * 2007-03-12 2008-09-25 Olympus Corp レーザー走査型顕微鏡
WO2011012646A2 (en) 2009-07-28 2011-02-03 F. Hoffmann-La Roche Ag Non-invasive in vivo optical imaging method
WO2012119999A1 (en) 2011-03-07 2012-09-13 F. Hoffmann-La Roche Ag Means and methods for in vivo testing of therapeutic antibodies
WO2012120004A1 (en) 2011-03-07 2012-09-13 F. Hoffmann-La Roche Ag In vivo selection of therapeutically active antibodies
US9423400B2 (en) 2011-04-20 2016-08-23 Korea Advanced Institute Of Science And Technology Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment
CN103608677A (zh) * 2011-04-20 2014-02-26 韩国科学技术院 在单分子水平上在细胞环境中分析蛋白-蛋白相互作用的方法和装置
US9377462B2 (en) 2011-04-20 2016-06-28 Korea Advanced Institute Of Science And Technology Method for analyzing protein-protein interaction on single-molecule level in cell environment, and method for measuring density of protein activated in cytosol
CN103582817A (zh) * 2011-04-20 2014-02-12 韩国科学技术院 在细胞环境中于单分子水平上分析蛋白-蛋白相互作用的方法，和测定在细胞溶质中激活的蛋白密度的方法
CN103608677B (zh) * 2011-04-20 2016-09-07 韩国科学技术院 在单分子水平上在细胞环境中分析蛋白-蛋白相互作用的方法和装置
US9733255B2 (en) 2011-04-20 2017-08-15 Korea Advanced Institute Of Science And Technology Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment
US9964544B2 (en) 2011-04-20 2018-05-08 Korea Advanced Institute Of Science And Technology Method and apparatus for analyzing protein-protein interaction on single-molecule level within the cellular environment
US10401367B2 (en) 2011-04-20 2019-09-03 Korea Advanced Institute Of Science And Technology Method and apparatus for analyzing protein-protein interaction on single molecule level within the cellular environment
WO2016116291A1 (de) * 2015-01-23 2016-07-28 Bayer Cropscience Ag Verfahren und vorrichtung zur ermittlung der wirkung von wirkstoffen an nematoden und anderen organismen in wässrigen tests
US10514375B2 (en) 2015-01-23 2019-12-24 Bayer Aktiengesellschaft Device and method for determining the action of active ingredients on nematodes and other organisms in aqueous tests
US10948481B2 (en) 2015-01-23 2021-03-16 Bayer Aktiengesellschaft Device and method for determining the action of active ingredients on nematodes and other organisms in aqueous tests
CN109509509A (zh) * 2018-09-29 2019-03-22 江西理工大学 基于动态加权蛋白质相互作用网络的蛋白质复合物挖掘方法
CN109509509B (zh) * 2018-09-29 2020-12-22 江西理工大学 基于动态加权蛋白质相互作用网络的蛋白质复合物挖掘方法

Also Published As

Publication number Publication date
JP2008538004A (ja) 2008-10-02
JP4921458B2 (ja) 2012-04-25
US20080206888A1 (en) 2008-08-28
EP1866443A1 (en) 2007-12-19
TW200704778A (en) 2007-02-01
US20060234279A1 (en) 2006-10-19
EP1866443A4 (en) 2009-04-08
US7674588B2 (en) 2010-03-09

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