JP4921458B2 - Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法 - Google Patents

Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法 Download PDF

Info

Publication number
JP4921458B2
JP4921458B2 JP2008505419A JP2008505419A JP4921458B2 JP 4921458 B2 JP4921458 B2 JP 4921458B2 JP 2008505419 A JP2008505419 A JP 2008505419A JP 2008505419 A JP2008505419 A JP 2008505419A JP 4921458 B2 JP4921458 B2 JP 4921458B2
Authority
JP
Japan
Prior art keywords
anisotropy
fluorophore
fret
donor
acceptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2008505419A
Other languages
English (en)
Japanese (ja)
Other versions
JP2008538004A (ja
JP2008538004A5 (enExample
Inventor
ミラー・スティーブン・シー．
コミタ・ポール・ビー．
シュメイト・クリストファー・ビー．
クロムウェル・エバン・エフ．
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Blueshift Biotechnologies Inc
Original Assignee
Blueshift Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Blueshift Biotechnologies Inc filed Critical Blueshift Biotechnologies Inc
Publication of JP2008538004A publication Critical patent/JP2008538004A/ja
Publication of JP2008538004A5 publication Critical patent/JP2008538004A5/ja
Application granted granted Critical
Publication of JP4921458B2 publication Critical patent/JP4921458B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6445—Measuring fluorescence polarisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2008505419A 2005-04-04 2006-04-03 Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法 Expired - Fee Related JP4921458B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US66842805P 2005-04-04 2005-04-04
US60/668,428 2005-04-04
US67884205P 2005-05-06 2005-05-06
US60/678,842 2005-05-06
PCT/US2006/012292 WO2006107864A1 (en) 2005-04-04 2006-04-03 Screening using polarization anisotropy in fret emissions

Publications (3)

Publication Number Publication Date
JP2008538004A JP2008538004A (ja) 2008-10-02
JP2008538004A5 JP2008538004A5 (enExample) 2009-05-21
JP4921458B2 true JP4921458B2 (ja) 2012-04-25

Family

ID=37073796

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2008505419A Expired - Fee Related JP4921458B2 (ja) 2005-04-04 2006-04-03 Ｆｒｅｔ発光における偏光異方性を使用したスクリーニングの方法

Country Status (5)

Country Link
US (2) US20060234279A1 (enExample)
EP (1) EP1866443A4 (enExample)
JP (1) JP4921458B2 (enExample)
TW (1) TW200704778A (enExample)
WO (1) WO2006107864A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006107864A1 (en) * 2005-04-04 2006-10-12 Blueshift Biotechnologies, Inc. Screening using polarization anisotropy in fret emissions
JP5189301B2 (ja) * 2007-03-12 2013-04-24 オリンパス株式会社 レーザー走査型顕微鏡
KR101679088B1 (ko) 2008-10-01 2016-12-06 셔윈 화 척추골의 전선-유도식 척추경 나사 고정 시스템 및 방법
US8568306B2 (en) 2009-04-03 2013-10-29 Mitchell A Hardenbrook Surgical retractor system
CA2768330A1 (en) 2009-07-28 2011-02-03 F. Hoffmann-La Roche Ag Non-invasive in vivo optical imaging method
AU2011232543B2 (en) 2010-03-26 2015-11-19 DISH Technologies L.L.C. Multiple input television receiver
EP2552333A4 (en) 2010-03-30 2014-11-26 Sherwin Hua SYSTEMS AND METHODS FOR PEDICULAR SCREW STABILIZATION OF SPINAL VERTEBRA
US8569254B2 (en) 2010-12-10 2013-10-29 National Yang Ming University Methods for modulating the expression and aggregation of CAG-expanded gene product in cells and methods for identifying agents useful for doing the same
JP6385060B2 (ja) 2011-03-07 2018-09-05 エフ・ホフマン−ラ・ロシュ・アクチェンゲゼルシャフト 治療的に活性な抗体のインビボにおける選択
US20140099264A1 (en) 2011-03-07 2014-04-10 F. Hoffman-La Roche Ag Means and methods for in vivo testing of therapeutic antibodies
KR101167649B1 (ko) * 2011-04-20 2012-07-20 한국과학기술원 세포 환경 내에서의 단일 분자 수준의 단백질-단백질 상호작용 분석 장치
EP2707714B1 (en) * 2011-05-09 2019-10-09 Whitehead Institute For Biomedical Research Chaperone interaction assays and uses thereof
JP5877741B2 (ja) * 2012-03-16 2016-03-08 株式会社日立製作所 細胞数モニタリング方法
WO2014059281A1 (en) * 2012-10-11 2014-04-17 The Regents Of The University Of Michigan Fluorescence polarization assay for detecting high molecular weight molecules in biological fluids
CN102967554B (zh) * 2012-10-29 2016-12-21 广东工业大学 一种双通道、单光路结构的荧光各向异性显微成像装置及方法
EP3404112B1 (en) * 2013-10-25 2020-08-26 BioMadison, Inc. Enzyme assay with duplicate fluorophores
WO2016116291A1 (de) 2015-01-23 2016-07-28 Bayer Cropscience Ag Verfahren und vorrichtung zur ermittlung der wirkung von wirkstoffen an nematoden und anderen organismen in wässrigen tests
CN109509509B (zh) * 2018-09-29 2020-12-22 江西理工大学 基于动态加权蛋白质相互作用网络的蛋白质复合物挖掘方法
US11160580B2 (en) 2019-04-24 2021-11-02 Spine23 Inc. Systems and methods for pedicle screw stabilization of spinal vertebrae
EP4065016B1 (en) 2019-11-27 2025-10-22 Spine23 Inc. Systems for treating a lateral curvature of a spine
US11782001B2 (en) 2020-12-04 2023-10-10 Attolight AG Dislocation type and density discrimination in semiconductor materials using cathodoluminescence measurements
TWI808554B (zh) * 2020-12-04 2023-07-11 亞光股份有限公司 使用陰極發光測量判別半導體材料中的位錯類型和密度的裝置與方法
EP4337119B1 (en) 2021-05-12 2025-12-10 Spine23 Inc. Systems for pedicle screw stabilization of spinal vertebrae

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5524405A (en) 1994-02-28 1996-06-11 Byrd; Randall Wall structure
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
US6573045B1 (en) * 1998-06-05 2003-06-03 Ribotargets, Ltd. Methods and kits for discovery of RNA-binding compounds
CA2377560C (en) * 1999-06-24 2010-08-24 Mcmaster University Incorporation and applications of biomolecular interactions within a carrier
US6509161B1 (en) * 2000-02-29 2003-01-21 Gentronix Limited Green fluorescent protein
WO2001088195A1 (en) * 2000-05-12 2001-11-22 Caliper Technologies Corp. Detection of nucleic acid hybridization by fluorescence polarization
JP2004537047A (ja) * 2001-06-14 2004-12-09 アナディーズ　ファーマスーティカルズ，アイエヌシー． 標的分子のリガンドをスクリーニングする方法
US20030228703A1 (en) * 2002-04-05 2003-12-11 The Regents Of The University Of Michigan Fluorescence resonance energy transfer quantitation and stoichiometry in living cells
US20030228603A1 (en) * 2002-04-05 2003-12-11 Cload Sharon T. Compositions selective for caffeine or aspartame and methods of using same
US6713262B2 (en) * 2002-06-25 2004-03-30 Agilent Technologies, Inc. Methods and compositions for high throughput identification of protein/nucleic acid binding pairs
JP2004093527A (ja) * 2002-09-04 2004-03-25 Center For Advanced Science & Technology Incubation Ltd 蛍光共鳴エネルギー転移を用いた蛋白質の分析方法
JP4214206B2 (ja) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Ｆｒｅｔを利用した蛍光指示薬
WO2005019811A2 (en) 2003-08-26 2005-03-03 Blueshift Biotechnologies, Inc. Time dependent fluorescence measurements
US20050264805A1 (en) 2004-02-09 2005-12-01 Blueshift Biotechnologies, Inc. Methods and apparatus for scanning small sample volumes
FR2872287B1 (fr) * 2004-06-28 2007-03-16 Cis Bio Internat Sa Procede d'amelioration de la detection des signaux de fluorescence lors d'un transfert d'energie non radiatif
JP2008505321A (ja) * 2004-07-02 2008-02-21 ブルーシフト・バイオテクノロジーズ・インコーポレーテッド 蛍光体微小環境の探索
US8031338B2 (en) * 2004-12-02 2011-10-04 Vanderbilt University Measuring Forster resonance energy transfer with polarized and depolarized light
WO2006107864A1 (en) * 2005-04-04 2006-10-12 Blueshift Biotechnologies, Inc. Screening using polarization anisotropy in fret emissions
JP5189301B2 (ja) * 2007-03-12 2013-04-24 オリンパス株式会社 レーザー走査型顕微鏡

Also Published As

Publication number Publication date
US20060234279A1 (en) 2006-10-19
US20080206888A1 (en) 2008-08-28
WO2006107864A1 (en) 2006-10-12
JP2008538004A (ja) 2008-10-02
TW200704778A (en) 2007-02-01
EP1866443A4 (en) 2009-04-08
EP1866443A1 (en) 2007-12-19
US7674588B2 (en) 2010-03-09

Similar Documents

Publication Publication Date Title
US7674588B2 (en) Screening using polarization anisotropy in FRET emissions
CN101432626B (zh) 用于肌钙蛋白分析的高灵敏系统和方法
Yasuda Imaging spatiotemporal dynamics of neuronal signaling using fluorescence resonance energy transfer and fluorescence lifetime imaging microscopy
Piston et al. Fluorescent protein FRET: the good, the bad and the ugly
JP3559525B2 (ja) 生成関数を用いて蛍光分子又は他の粒子を特徴付ける方法
JP2012514184A (ja) 多重分析物の検出及び定量化のための方法
JP2010530069A (ja) 標識不使用の結合検出機能と蛍光増幅機能を兼備する格子型センサおよびセンサ用読取りシステム
US20210255195A1 (en) High precision spectral fret assays
Tan et al. Application of photonic crystal enhanced fluorescence to detection of low serum concentrations of human IgE antibodies specific for a purified cat allergen (Fel D1)
US20120258553A1 (en) Analyte measurement apparatus and method
JP5057377B2 (ja) 生体成分又はその機能の測定方法及び装置
EP1797195B1 (en) Method and apparatus for the detection of labeling elements in a sample
US20190049377A1 (en) Bioaffinity assay method utilizing two-photonexcitation of fluorescence
Hunter et al. High-throughput investigation of macromolecular interactions for drug development using spectral shift technology
US20250034629A1 (en) Method for Dynamic Range Expansion for Multiplex Assays
US20050191705A1 (en) High throughput screening using fluorophore labeled lipid membranes and fluorescence correlation spectroscopy
JP2010256010A (ja) 蛍光分析による抗原抗体反応検出方法
WO2008080441A1 (en) Micropatterning of biomolecules
US20050124008A1 (en) Homogeneous competition assays
Matthews et al. A high-content screening platform utilizing polarization anisotropy and FLIM microscopy
WO2023057735A1 (en) Apparatus and method for multi-characteristic measurement of radiation properties
EP3317643B1 (en) Modulation of luminescent dyes
Grigaravicius et al. Protein Chip analysis by probing time-resolved UV-fluorescence
Hayden et al. Advanced microscopy: time-resolved multi-spectral imaging of single biomolecules.
KR20220153579A (ko) Fret 현미경 검사에서 사용하기 위한 시스템 및 방법

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20090331

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20090331

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20110530

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110927

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111220

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20120124

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20120202

R150 Certificate of patent or registration of utility model

Ref document number: 4921458

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20150210

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees