WO2006100484A2 - Deliver of molecules to a li id bila - Google Patents

Deliver of molecules to a li id bila Download PDF

Info

Publication number
WO2006100484A2
WO2006100484A2 PCT/GB2006/001057 GB2006001057W WO2006100484A2 WO 2006100484 A2 WO2006100484 A2 WO 2006100484A2 GB 2006001057 W GB2006001057 W GB 2006001057W WO 2006100484 A2 WO2006100484 A2 WO 2006100484A2
Authority
WO
WIPO (PCT)
Prior art keywords
lipid bilayer
probe
carrier surface
membrane protein
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2006/001057
Other languages
English (en)
French (fr)
Other versions
WO2006100484A3 (en
Inventor
Matthew Alexander Holden
John Hagan Pryce Bayley
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford University Innovation Ltd
Original Assignee
Oxford University Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford University Innovation Ltd filed Critical Oxford University Innovation Ltd
Priority to AT06726479T priority Critical patent/ATE473438T1/de
Priority to JP2008502471A priority patent/JP5253148B2/ja
Priority to US11/884,927 priority patent/US7939270B2/en
Priority to EP06726479A priority patent/EP1861071B1/en
Priority to DE602006015292T priority patent/DE602006015292D1/de
Publication of WO2006100484A2 publication Critical patent/WO2006100484A2/en
Anticipated expiration legal-status Critical
Publication of WO2006100484A3 publication Critical patent/WO2006100484A3/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion

Definitions

  • the present method has particular application to insertion of a membrane protein, as the molecule, into a lipid bilayer.
  • the method may be applied to a stochastic sensing technique, for example in which the molecule is a membrane protein which is a pore or a channel and the apparatus further comprises an electrical circuit capable of measuring an electrical signal across the lipid bilayer
  • an apparatus for performing stochastic sensing with a membrane protein comprising: a chamber body defining a chamber capable of containing an aqueous solution, a wall of the chamber having an aperture capable of supporting a lipid bilayer across the aperture; a probe capable of holding the molecule on a carrier surface thereof, the probe being movably mounted on the chamber body to allow movement of the carrier surface against the aperture for engagement of the carrier surface against a lipid bilayer supported across the aperture.
  • the probe 10 is moved to engage the protruding surface 15 of the gel 14 against the lipid bilayer 9 extending across the aperture 7 as shown by the arrow in Fig. 3. This movement of the probe 10 is performed simply by manual manipulation of the boom 16.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicinal Preparation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
PCT/GB2006/001057 2005-03-23 2006-03-22 Deliver of molecules to a li id bila Ceased WO2006100484A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT06726479T ATE473438T1 (de) 2005-03-23 2006-03-22 Zuführung von molekülen an eine lipiddoppelschicht
JP2008502471A JP5253148B2 (ja) 2005-03-23 2006-03-22 脂質二重層への分子の送達
US11/884,927 US7939270B2 (en) 2005-03-23 2006-03-22 Delivery of molecules to a lipid bilayer
EP06726479A EP1861071B1 (en) 2005-03-23 2006-03-22 Deliver of molecules to a lipid bilayer
DE602006015292T DE602006015292D1 (de) 2005-03-23 2006-03-22 Zuführung von molekülen an eine lipiddoppelschicht

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0505971.2 2005-03-23
GBGB0505971.2A GB0505971D0 (en) 2005-03-23 2005-03-23 Delivery of molecules to a lipid bilayer

Publications (2)

Publication Number Publication Date
WO2006100484A2 true WO2006100484A2 (en) 2006-09-28
WO2006100484A3 WO2006100484A3 (en) 2008-05-29

Family

ID=34531741

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2006/001057 Ceased WO2006100484A2 (en) 2005-03-23 2006-03-22 Deliver of molecules to a li id bila

Country Status (7)

Country Link
US (1) US7939270B2 (enExample)
EP (1) EP1861071B1 (enExample)
JP (1) JP5253148B2 (enExample)
AT (1) ATE473438T1 (enExample)
DE (1) DE602006015292D1 (enExample)
GB (1) GB0505971D0 (enExample)
WO (1) WO2006100484A2 (enExample)

Cited By (134)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2446823A (en) * 2007-02-20 2008-08-27 Oxford Nanolabs Ltd Formulation of lipid bilayers
WO2008102121A1 (en) * 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
GB2447043A (en) * 2007-02-20 2008-09-03 Oxford Nanolabs Ltd Lipid bilayer sensor system
WO2010086602A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
US8124191B2 (en) * 2007-11-30 2012-02-28 Electronic Bio Sciences, Llc Method and apparatus for single side bilayer formation
WO2012033524A2 (en) 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
WO2012095660A2 (en) 2011-01-12 2012-07-19 Isis Innovation Limited Method using fluorinated amphiphiles
WO2012107778A2 (en) 2011-02-11 2012-08-16 Oxford Nanopore Technologies Limited Mutant pores
WO2012164270A1 (en) 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
WO2013057495A2 (en) 2011-10-21 2013-04-25 Oxford Nanopore Technologies Limited Enzyme method
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
WO2013121224A1 (en) 2012-02-16 2013-08-22 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
EP2682460A1 (en) 2008-07-07 2014-01-08 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2014013260A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Modified helicases
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
WO2014064444A1 (en) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Droplet interfaces
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
US8785211B2 (en) 2005-11-15 2014-07-22 Isis Innovation Limited Methods using pores
US8784929B2 (en) 2007-08-21 2014-07-22 Isis Innovation Limited Bilayers
US8822160B2 (en) 2007-10-05 2014-09-02 Isis Innovation Limited Molecular adaptors
WO2014135838A1 (en) 2013-03-08 2014-09-12 Oxford Nanopore Technologies Limited Enzyme stalling method
WO2015056028A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Method of characterizing a target ribonucleic acid (rna) comprising forming a complementary polynucleotide which moves through a transmembrane pore
WO2015055981A2 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified enzymes
WO2015081178A1 (en) 2013-11-26 2015-06-04 Illumina, Inc. Compositions and methods for polynucleotide sequencing
EP2886663A1 (en) 2013-12-19 2015-06-24 Centre National de la Recherche Scientifique (CNRS) Nanopore sequencing using replicative polymerases and helicases
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
WO2015150787A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method of target molecule characterisation using a molecular pore
WO2016055777A2 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Modified enzymes
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
WO2016059375A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Methods for delivering an analyte to transmembrane pores
WO2016059363A1 (en) 2014-10-14 2016-04-21 Oxford Nanopore Technologies Limited Method
US9415422B2 (en) 2006-03-10 2016-08-16 Siemens Industry, Inc. Mail sorting system
WO2016132123A1 (en) 2015-02-19 2016-08-25 Oxford Nanopore Technologies Limited Hetero-pores
WO2016132124A1 (en) 2015-02-19 2016-08-25 Oxford Nanopore Technologies Limited Method
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
US9562887B2 (en) 2008-11-14 2017-02-07 Oxford University Innovation Limited Methods of enhancing translocation of charged analytes through transmembrane protein pores
WO2017098322A1 (en) 2015-12-08 2017-06-15 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
US9732381B2 (en) 2009-03-25 2017-08-15 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence
WO2017149317A1 (en) 2016-03-02 2017-09-08 Oxford Nanopore Technologies Limited Mutant pore
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
US9797013B2 (en) 2007-04-04 2017-10-24 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
WO2017203269A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method of nanopore sequencing of concatenaded nucleic acids
US9927398B2 (en) 2007-12-19 2018-03-27 Oxford Nanopore Technologies Ltd. Formation of layers of amphiphilic molecules
WO2018060740A1 (en) 2016-09-29 2018-04-05 Oxford Nanopore Technologies Limited Method for nucleic acid detection by guiding through a nanopore
WO2018073604A1 (en) 2016-10-21 2018-04-26 Oxford Nanopore Technologies Limited Method
WO2018100370A1 (en) 2016-12-01 2018-06-07 Oxford Nanopore Technologies Limited Methods and systems for characterizing analytes using nanopores
US10006905B2 (en) 2013-03-25 2018-06-26 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
WO2018146491A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
WO2018203071A1 (en) 2017-05-04 2018-11-08 Oxford Nanopore Technologies Limited Method of determining the presence or absence of a target analyte comprising using a reporter polynucleotide and a transmembrane pore
US10131943B2 (en) 2012-12-19 2018-11-20 Oxford Nanopore Technologies Ltd. Analysis of a polynucleotide via a nanopore system
WO2018211241A1 (en) 2017-05-04 2018-11-22 Oxford Nanopore Technologies Limited Transmembrane pore consisting of two csgg pores
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
US10167503B2 (en) 2014-05-02 2019-01-01 Oxford Nanopore Technologies Ltd. Mutant pores
WO2019081442A1 (en) * 2017-10-23 2019-05-02 F. Hoffmann-La Roche Ag REMOVAL AND REINTEGRATION OF PROTEIN NANOPORES IN A MEMBRANE USING AN OSMOTIC IMBALANCE
US10338056B2 (en) 2012-02-13 2019-07-02 Oxford Nanopore Technologies Ltd. Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
US10343350B2 (en) 2010-02-08 2019-07-09 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10392658B2 (en) 2014-01-22 2019-08-27 Oxford Nanopore Technologies Ltd. Method for controlling the movement of a polynucleotide through a transmembrane pore
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
GB201913997D0 (en) 2019-09-27 2019-11-13 Oxford Nanopore Tech Ltd Method
WO2019224558A1 (en) 2018-05-24 2019-11-28 Oxford Nanopore Technologies Limited Method for selecting polynucleotides based on the size in a globular form
US10501767B2 (en) 2013-08-16 2019-12-10 Oxford Nanopore Technologies Ltd. Polynucleotide modification methods
WO2019234432A1 (en) 2018-06-06 2019-12-12 Oxford Nanopore Technologies Limited Method
US10519499B2 (en) 2014-07-31 2019-12-31 Illumina, Inc. Hybrid nanopore sensors
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
WO2020016573A1 (en) 2018-07-16 2020-01-23 Oxford University Innovation Limited Molecular hopper
US10549274B2 (en) 2014-10-17 2020-02-04 Oxford Nanopore Technologies Ltd. Electrical device with detachable components
WO2020025909A1 (en) 2018-07-30 2020-02-06 Oxford University Innovation Limited Assemblies
WO2020095052A1 (en) 2018-11-08 2020-05-14 Oxford Nanopore Technologies Limited Pore
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
US10669578B2 (en) 2014-02-21 2020-06-02 Oxford Nanopore Technologies Ltd. Sample preparation method
US10689697B2 (en) 2014-10-16 2020-06-23 Oxford Nanopore Technologies Ltd. Analysis of a polymer
WO2020128517A1 (en) 2018-12-21 2020-06-25 Oxford Nanopore Technologies Limited Method of encoding data on a polynucleotide strand
US10712254B2 (en) 2013-09-23 2020-07-14 Oxford University Innovation Limited Method of analyzing post-translational modifications
WO2020183172A1 (en) 2019-03-12 2020-09-17 Oxford Nanopore Technologies Inc. Nanopore sensing device and methods of operation and of forming it
WO2020188235A1 (en) 2019-03-19 2020-09-24 Oxford Nanopore Technologies Limited Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
WO2020208357A1 (en) 2019-04-09 2020-10-15 Oxford Nanopore Technologies Limited Pore
US10814298B2 (en) 2012-10-26 2020-10-27 Oxford Nanopore Technologies Ltd. Formation of array of membranes and apparatus therefor
WO2021078971A2 (en) 2019-10-25 2021-04-29 Oxford Nanopore Technologies Limited Improved nanopore sensing device, components and method of manufacture
WO2021111125A1 (en) 2019-12-02 2021-06-10 Oxford Nanopore Technologies Limited Method of characterising a target polypeptide using a nanopore
WO2021111139A1 (en) 2019-12-04 2021-06-10 Oxford Nanopore Technologies Limited Method
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
WO2021255477A1 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited Method of repeatedly moving a double-stranded polynucleotide through a nanopore
WO2021255476A2 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited Method
WO2021255475A1 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited A method of selectively characterising a polynucleotide using a detector
WO2021255414A1 (en) 2020-06-17 2021-12-23 Oxford Nanopore Technologies Limited Nanopore support structure and manufacture thereof
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
WO2022074397A1 (en) 2020-10-08 2022-04-14 Oxford Nanopore Technologies Limited Modification of a nanopore forming protein oligomer
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
US11352664B2 (en) 2009-01-30 2022-06-07 Oxford Nanopore Technologies Plc Adaptors for nucleic acid constructs in transmembrane sequencing
EP4063521A1 (en) 2016-05-25 2022-09-28 Oxford Nanopore Technologies PLC Method of nanopore sequencing
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
US11572387B2 (en) 2017-06-30 2023-02-07 Vib Vzw Protein pores
WO2023026056A1 (en) 2021-08-26 2023-03-02 Oxford Nanopore Technologies Plc Nanopore
US11596940B2 (en) 2016-07-06 2023-03-07 Oxford Nanopore Technologies Plc Microfluidic device
US11649480B2 (en) 2016-05-25 2023-05-16 Oxford Nanopore Technologies Plc Method for modifying a template double stranded polynucleotide
WO2023118404A1 (en) 2021-12-23 2023-06-29 Oxford Nanopore Technologies Plc Pore
WO2023118891A1 (en) 2021-12-23 2023-06-29 Oxford Nanopore Technologies Plc Method of characterising polypeptides using a nanopore
WO2023118892A1 (en) 2021-12-23 2023-06-29 Oxford Nanopore Technologies Plc Method
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
US11725205B2 (en) 2018-05-14 2023-08-15 Oxford Nanopore Technologies Plc Methods and polynucleotides for amplifying a target polynucleotide
WO2023198911A2 (en) 2022-04-14 2023-10-19 Oxford Nanopore Technologies Plc Novel modified protein pores and enzymes
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
WO2024033447A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc De novo pores
WO2024033421A2 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024033443A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024033422A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024089270A2 (en) 2022-10-28 2024-05-02 Oxford Nanopore Technologies Plc Pore monomers and pores
WO2024094986A1 (en) 2022-10-31 2024-05-10 Oxford Nanopore Technologies Plc Method
WO2024100270A1 (en) 2022-11-11 2024-05-16 Oxford Nanopore Technologies Plc Novel pore monomers and pores
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
WO2024165853A1 (en) 2023-02-07 2024-08-15 Oxford University Innovation Limited Method of characterising a peptide, polypeptide or protein using a nanopore
WO2024200280A1 (en) 2023-03-24 2024-10-03 Oxford Nanopore Technologies Plc Method and kits
WO2024213802A1 (en) 2023-04-14 2024-10-17 Oxford Nanopore Technologies Plc Novel pore monomers and pores
US12121894B2 (en) 2017-11-29 2024-10-22 Oxford Nanopore Technologies Plc Microfluidic device
WO2024235919A1 (en) 2023-05-12 2024-11-21 Oxford Nanopore Technologies Plc Modified helicases
US12195777B2 (en) 2018-02-02 2025-01-14 Oxford Nanopore Technologies Plc Polynucleotide synthesis method, kit and system
WO2025040887A1 (en) 2023-08-18 2025-02-27 Oxford University Innovation Limited Nanopore
WO2025046221A1 (en) 2023-08-25 2025-03-06 Oxford University Innovation Limited Power unit comprising distinct droplets separated by an amphiphilic membrane
WO2025083247A1 (en) 2023-10-18 2025-04-24 Oxford Nanopore Technologies Plc Novel enzymes
WO2025099094A1 (en) 2023-11-06 2025-05-15 Oxford Nanopore Technologies Plc Method
WO2025228949A1 (en) 2024-04-29 2025-11-06 Oxford Nanopore Technologies Plc Photo-active conjugates and their uses
WO2025238370A1 (en) 2024-05-17 2025-11-20 Oxford Nanopore Technologies Plc Enhanced polynucleotide sequencing
WO2025242713A1 (en) 2024-05-21 2025-11-27 Oxford Nanopore Technologies Plc A method for sequencing chromatin
US12503729B2 (en) 2022-05-31 2025-12-23 Oxford Nanopore Technologies Plc Enzyme method

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4159298B2 (ja) 2001-09-04 2008-10-01 シャープ株式会社 電気掃除機及び機器
US9233846B2 (en) 2005-10-14 2016-01-12 The Regents Of The University Of California Formation and encapsulation of molecular bilayer and monolayer membranes
CN101495656B (zh) * 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
US8772041B2 (en) * 2008-05-22 2014-07-08 The Regents Of The University Of California Membrane precursors and membranes formed therefrom
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
WO2012083249A2 (en) 2010-12-17 2012-06-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using modified nucleotides and nanopore detection
GB2500360B (en) 2010-12-22 2019-10-23 Genia Tech Inc Nanopore-based single DNA molecule characterization, identification and isolation using speed bumps
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US8968539B2 (en) 2011-03-08 2015-03-03 Electronic Biosciences, Inc. Methods for voltage-induced protein incorporation into planar lipid bilayers
EP2578207A3 (en) * 2011-10-05 2015-10-07 Jacob J. Schmidt Masking apertures enabling automation and solution exchange in sessile droplet lipid bilayers
GB201119032D0 (en) 2011-11-03 2011-12-14 Isis Innovation Multisomes: encapsulated droplet networks
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
ES2906186T3 (es) 2012-04-09 2022-04-13 Univ Columbia Método para la preparación de nanoporo y usos del mismo
EP2861768A4 (en) 2012-06-15 2016-03-02 Genia Technologies Inc CHIP SETUP AND HIGH ACCURACY NUCLEIC ACID SEQUENCING
WO2013191793A1 (en) 2012-06-20 2013-12-27 The Trustees Of Columbia University In The City Of New York Nucleic acid sequencing by nanopore detection of tag molecules
GB201219196D0 (en) 2012-10-25 2012-12-12 Isis Innovation Droplet assembly method
GB201219201D0 (en) 2012-10-25 2012-12-12 Isis Innovation Hydrogel network
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
EP2928610A2 (en) * 2012-12-07 2015-10-14 Isis Innovation Limited Droplet assembly by 3d printing
EP2746772B1 (en) 2012-12-20 2016-03-23 AIT Austrian Institute of Technology GmbH Lipid membrane enveloped particles with membrane proteins
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
EP3640349A3 (en) 2013-10-23 2020-07-29 Roche Sequencing Solutions, Inc. High speed molecular sensing with nanopores
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
CN106715453B (zh) 2014-03-24 2021-04-30 哥伦比亚大学董事会 用于生产带标签的核苷酸的化学方法
KR102329836B1 (ko) 2015-01-26 2021-11-19 셀렉티스 암 면역치료를 위한 항-CLL1 특이적 단일-체인 키메라 항원 수용체들(scCARS)
JP2018059786A (ja) * 2016-10-04 2018-04-12 住友化学株式会社 嗅覚受容体複合体を用いた標的物質の検出装置及び検出方法並びに該検出装置の製造方法
WO2025160387A1 (en) * 2024-01-24 2025-07-31 The Rockefeller University Microscope for freestanding bilayer illumination

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3799743A (en) 1971-11-22 1974-03-26 Alexander James Stable lysis responsive lipid bilayer
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
GB9712386D0 (en) * 1997-06-14 1997-08-13 Univ Coventry Biosensor
WO1999041401A1 (en) * 1998-02-17 1999-08-19 University Of Wales College Of Medicine Method and apparatus for introducing substances into the cell plasma membrane and/or cytosol
CN1325405A (zh) * 1998-08-31 2001-12-05 格莱风科学公司 膜多肽的脂基质协助的化学结合和合成
JP3665720B2 (ja) * 1999-09-24 2005-06-29 株式会社東芝 センサデバイス
US20040096895A1 (en) * 2001-01-18 2004-05-20 Lakey Jeremy Hugh Biosensor with covalently attached membrane-spanning proteins
AU2003245272A1 (en) 2002-05-10 2003-11-11 The Texas A And M University System Stochastic sensing through covalent interactions
JP4394916B2 (ja) * 2003-09-19 2010-01-06 独立行政法人科学技術振興機構 人工脂質二重膜の形成装置および人工脂質二重膜の形成方法、並びにその利用
EP1712909B1 (en) * 2004-01-21 2012-09-19 Japan Science and Technology Agency Method of forming planar lipid double membrane for membrane protein analysis and apparatus therefor

Cited By (308)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8785211B2 (en) 2005-11-15 2014-07-22 Isis Innovation Limited Methods using pores
US9415422B2 (en) 2006-03-10 2016-08-16 Siemens Industry, Inc. Mail sorting system
GB2446823A (en) * 2007-02-20 2008-08-27 Oxford Nanolabs Ltd Formulation of lipid bilayers
WO2008102121A1 (en) * 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Formation of lipid bilayers
GB2447043A (en) * 2007-02-20 2008-09-03 Oxford Nanolabs Ltd Lipid bilayer sensor system
US20100196203A1 (en) * 2007-02-20 2010-08-05 Gurdial Singh Sanghera Formation of Lipid Bilayers
US10215768B2 (en) 2007-02-20 2019-02-26 Oxford Nanopore Technologies Ltd. Lipid bilayer sensor system
US10344327B2 (en) 2007-04-04 2019-07-09 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US9797013B2 (en) 2007-04-04 2017-10-24 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US12054775B2 (en) 2007-04-04 2024-08-06 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US10059988B2 (en) 2007-04-04 2018-08-28 The Regents Of The University Of California Methods for using a nanopore
US10081835B2 (en) 2007-04-04 2018-09-25 The Regents Of The University Of California Nucleotide sequencing using an array of independently addressable nanopores
US11970738B2 (en) 2007-04-04 2024-04-30 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US10196688B2 (en) 2007-04-04 2019-02-05 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US10202645B2 (en) 2007-04-04 2019-02-12 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US10208342B2 (en) 2007-04-04 2019-02-19 The Regents Of The University Of California Compositions, devices, systems, and methods for using a nanopore
US8784929B2 (en) 2007-08-21 2014-07-22 Isis Innovation Limited Bilayers
US8822160B2 (en) 2007-10-05 2014-09-02 Isis Innovation Limited Molecular adaptors
US8707892B2 (en) 2007-11-30 2014-04-29 Electronic Biosciences, Inc. Method and apparatus for single side bilayer formation
US8334024B1 (en) 2007-11-30 2012-12-18 Electronic Bio Sciences, Llc Method and apparatus for single side bilayer formation
US8124191B2 (en) * 2007-11-30 2012-02-28 Electronic Bio Sciences, Llc Method and apparatus for single side bilayer formation
US12140563B2 (en) 2007-12-19 2024-11-12 Oxford Nanopore Technologies Plc Formation of layers of amphiphilic molecules
US10416117B2 (en) 2007-12-19 2019-09-17 Oxford Nanopore Technologies Ltd. Formation of layers of amphiphilic molecules
US9927398B2 (en) 2007-12-19 2018-03-27 Oxford Nanopore Technologies Ltd. Formation of layers of amphiphilic molecules
US12467898B2 (en) 2007-12-19 2025-11-11 Oxford Nanopore Technologies Plc Nanopore arrays for sequencing nucleic acids
US11898984B2 (en) 2007-12-19 2024-02-13 Oxford Nanopore Technologies Plc Nanopore arrays for sequencing nucleic acids
US11859247B2 (en) 2008-07-07 2024-01-02 Oxford Nanopore Technologies Plc Enzyme-pore constructs
US9885078B2 (en) 2008-07-07 2018-02-06 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US10077471B2 (en) 2008-07-07 2018-09-18 Oxford Nanopore Technologies Ltd. Enzyme-pore constructs
US11078530B2 (en) 2008-07-07 2021-08-03 Oxford Nanopore Technologies Ltd. Enzyme-pore constructs
EP2682460A1 (en) 2008-07-07 2014-01-08 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US9562887B2 (en) 2008-11-14 2017-02-07 Oxford University Innovation Limited Methods of enhancing translocation of charged analytes through transmembrane protein pores
US11352664B2 (en) 2009-01-30 2022-06-07 Oxford Nanopore Technologies Plc Adaptors for nucleic acid constructs in transmembrane sequencing
US11459606B2 (en) 2009-01-30 2022-10-04 Oxford Nanopore Technologies Plc Adaptors for nucleic acid constructs in transmembrane sequencing
WO2010086603A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Enzyme mutant
WO2010086602A1 (en) 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Hybridization linkers
EP3677691A1 (en) 2009-03-25 2020-07-08 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence
US12173364B2 (en) 2009-03-25 2024-12-24 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence
US9732381B2 (en) 2009-03-25 2017-08-15 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence
US10669581B2 (en) 2009-03-25 2020-06-02 Oxford University Innovation Limited Method for sequencing a heteropolymeric target nucleic acid sequence
US10343350B2 (en) 2010-02-08 2019-07-09 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10926486B2 (en) 2010-02-08 2021-02-23 Roche Sequencing Solutions, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US10456993B2 (en) 2010-02-08 2019-10-29 Roche Sequencing Solutions, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US11027502B2 (en) 2010-02-08 2021-06-08 Roche Sequencing Solutions, Inc. Systems and methods for forming a nanopore in a lipid bilayer
WO2012033524A2 (en) 2010-09-07 2012-03-15 The Regents Of The University Of California Control of dna movement in a nanopore at one nucleotide precision by a processive enzyme
WO2012095660A2 (en) 2011-01-12 2012-07-19 Isis Innovation Limited Method using fluorinated amphiphiles
WO2012107778A2 (en) 2011-02-11 2012-08-16 Oxford Nanopore Technologies Limited Mutant pores
US9751915B2 (en) 2011-02-11 2017-09-05 Oxford Nanopore Technologies Ltd. Mutant pores
US12473595B2 (en) 2011-05-27 2025-11-18 Oxford Nanopore Technologies Plc Coupling method
EP3633370A1 (en) 2011-05-27 2020-04-08 Oxford Nanopore Technologies Limited Coupling method
WO2012164270A1 (en) 2011-05-27 2012-12-06 Oxford Nanopore Technologies Limited Coupling method
US11041194B2 (en) 2011-05-27 2021-06-22 Oxford Nanopore Technologies Ltd. Coupling method
US11946102B2 (en) 2011-05-27 2024-04-02 Oxford Nanopore Technologies Plc Coupling method
US11959135B2 (en) 2011-05-27 2024-04-16 Oxford Nanopore Technologies Plc Coupling method
US11136623B2 (en) 2011-05-27 2021-10-05 Oxford Nanopore Technologies Limited Coupling method
EP4273092A2 (en) 2011-05-27 2023-11-08 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
US10246741B2 (en) 2011-05-27 2019-04-02 Oxford Nanopore Technologies Ltd. Coupling method
EP3848706A1 (en) 2011-05-27 2021-07-14 Oxford Nanopore Technologies Limited Coupling method
US12152276B2 (en) 2011-05-27 2024-11-26 Oxford Nanopore Technologies Plc Coupling method
US11168363B2 (en) 2011-07-25 2021-11-09 Oxford Nanopore Technologies Ltd. Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US12168799B2 (en) 2011-07-25 2024-12-17 Oxford Nanopore Technologies Plc Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US10851409B2 (en) 2011-07-25 2020-12-01 Oxford Nanopore Technologies Ltd. Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US10597713B2 (en) 2011-07-25 2020-03-24 Oxford Nanopore Technologies Ltd. Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US11261487B2 (en) 2011-07-25 2022-03-01 Oxford Nanopore Technologies Plc Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
WO2013014451A1 (en) 2011-07-25 2013-01-31 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9957560B2 (en) 2011-07-25 2018-05-01 Oxford Nanopore Technologies Ltd. Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US10724087B2 (en) 2011-10-21 2020-07-28 Oxford Nanopore Technologies Ltd. Enzyme method
WO2013057495A2 (en) 2011-10-21 2013-04-25 Oxford Nanopore Technologies Limited Enzyme method
EP2987870A1 (en) 2011-10-21 2016-02-24 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a transmembrane pore and molecular motor
US11634763B2 (en) 2011-10-21 2023-04-25 Oxford Nanopore Technologies Plc Enzyme method
US9758823B2 (en) 2011-10-21 2017-09-12 Oxford Nanopore Technologies Limited Enzyme method
US10385382B2 (en) 2011-12-29 2019-08-20 Oxford Nanopore Technologies Ltd. Enzyme method
WO2013098561A1 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
US9617591B2 (en) 2011-12-29 2017-04-11 Oxford Nanopore Technologies Ltd. Method for characterising a polynucleotide by using a XPD helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
US11561216B2 (en) 2012-02-13 2023-01-24 Oxford Nanopore Technologies Plc Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
US10338056B2 (en) 2012-02-13 2019-07-02 Oxford Nanopore Technologies Ltd. Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
US11913936B2 (en) 2012-02-13 2024-02-27 Oxford Nanopore Technologies Plc Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
WO2013121201A1 (en) 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
US10739341B2 (en) 2012-02-15 2020-08-11 Oxford Nanopore Technologies Limited Aptamer method
WO2013121224A1 (en) 2012-02-16 2013-08-22 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
EP3736339A1 (en) 2012-02-16 2020-11-11 Oxford Nanopore Technologies Limited Analysis of measurements of a polymer
US11959906B2 (en) 2012-02-16 2024-04-16 Oxford Nanopore Technologies Plc Analysis of measurements of a polymer
EP3192804A1 (en) 2012-04-10 2017-07-19 Oxford Nanopore Technologies Limited Mutant lysenin pores
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
US11845780B2 (en) 2012-04-10 2023-12-19 Oxford Nanopore Technologies Plc Mutant lysenin pores
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
US12258375B2 (en) 2012-04-10 2025-03-25 Oxford Nanopore Technologies Plc Mutant lysenin pores
US10882889B2 (en) 2012-04-10 2021-01-05 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
WO2014013262A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Enzyme construct
US9797009B2 (en) 2012-07-19 2017-10-24 Oxford Nanopore Technologies Limited Enzyme construct
US12252717B2 (en) 2012-07-19 2025-03-18 Oxford Nanopore Technologies Plc Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
US10808231B2 (en) 2012-07-19 2020-10-20 Oxford Nanopore Technologies Limited Modified helicases
WO2014013260A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Modified helicases
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
US12448646B2 (en) 2012-07-19 2025-10-21 Oxford Nanopore Technologies Plc SSB method
US11525126B2 (en) 2012-07-19 2022-12-13 Oxford Nanopore Technologies Plc Modified helicases
EP3741847A1 (en) 2012-07-19 2020-11-25 Oxford Nanopore Technologies Limited Modified helicases
US9551023B2 (en) 2012-09-14 2017-01-24 Oxford Nanopore Technologies Ltd. Sample preparation method
US10814298B2 (en) 2012-10-26 2020-10-27 Oxford Nanopore Technologies Ltd. Formation of array of membranes and apparatus therefor
US12458945B2 (en) 2012-10-26 2025-11-04 Oxford Nanopore Technologies Plc Formation of array of membranes and apparatus therefor
US12350637B2 (en) 2012-10-26 2025-07-08 Oxford Nanopore Technologies Plc Formation of array of membranes and apparatus therefor
WO2014064444A1 (en) 2012-10-26 2014-05-01 Oxford Nanopore Technologies Limited Droplet interfaces
US9995728B2 (en) 2012-11-06 2018-06-12 Oxford Nanopore Technologies Ltd. Quadruplex method
WO2014072703A1 (en) 2012-11-06 2014-05-15 Oxford Nanopore Technologies Limited Quadruplex method
US12486534B2 (en) 2012-12-19 2025-12-02 Oxford Nanopore Technologies Plc Analysis of a polynucleotide via a nanopore system
US12351867B2 (en) 2012-12-19 2025-07-08 Oxford Nanopore Technologies Plc Analysis of a polynucleotide via a nanopore system
US11085077B2 (en) 2012-12-19 2021-08-10 Oxford Nanopore Technologies Ltd. Analysis of a polynucleotide via a nanopore system
US10131943B2 (en) 2012-12-19 2018-11-20 Oxford Nanopore Technologies Ltd. Analysis of a polynucleotide via a nanopore system
US10221450B2 (en) 2013-03-08 2019-03-05 Oxford Nanopore Technologies Ltd. Enzyme stalling method
WO2014135838A1 (en) 2013-03-08 2014-09-12 Oxford Nanopore Technologies Limited Enzyme stalling method
US11560589B2 (en) 2013-03-08 2023-01-24 Oxford Nanopore Technologies Plc Enzyme stalling method
US10802015B2 (en) 2013-03-25 2020-10-13 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10006905B2 (en) 2013-03-25 2018-06-26 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10514378B2 (en) 2013-03-25 2019-12-24 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US11761956B2 (en) 2013-03-25 2023-09-19 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10976311B2 (en) 2013-03-25 2021-04-13 Katholieke Universiteit Leuven Nanopore biosensors for detection of proteins and nucleic acids
US10501767B2 (en) 2013-08-16 2019-12-10 Oxford Nanopore Technologies Ltd. Polynucleotide modification methods
US11186857B2 (en) 2013-08-16 2021-11-30 Oxford Nanopore Technologies Plc Polynucleotide modification methods
US11592382B2 (en) 2013-09-23 2023-02-28 Oxford University Innovation Limited Method of analyzing post-translational modifications
US10712254B2 (en) 2013-09-23 2020-07-14 Oxford University Innovation Limited Method of analyzing post-translational modifications
WO2015056028A1 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Method of characterizing a target ribonucleic acid (rna) comprising forming a complementary polynucleotide which moves through a transmembrane pore
US12258591B2 (en) 2013-10-18 2025-03-25 Oxford Nanopore Technologies Plc Modified helicases
US10724018B2 (en) 2013-10-18 2020-07-28 Oxford Nanopore Technologies Ltd. Modified helicases
WO2015055981A2 (en) 2013-10-18 2015-04-23 Oxford Nanopore Technologies Limited Modified enzymes
US11111532B2 (en) 2013-10-18 2021-09-07 Oxford Nanopore Technologies Ltd. Method of characterizing a target ribonucleic acid (RNA) comprising forming a complementary polynucleotide which moves through a transmembrane pore
US11525125B2 (en) 2013-10-18 2022-12-13 Oxford Nanopore Technologies Plc Modified helicases
EP3575410A2 (en) 2013-10-18 2019-12-04 Oxford Nanopore Technologies Limited Modified enzymes
EP4006168A1 (en) 2013-10-18 2022-06-01 Oxford Nanopore Technologies PLC Method of characterizing a target ribonucleic acid (rna) comprising forming a complementary polynucleotide which moves through a transmembrane pore
US11879155B2 (en) 2013-11-26 2024-01-23 Illumina, Inc. Compositions and methods for polynucleotide sequencing
EP4282983A2 (en) 2013-11-26 2023-11-29 Illumina, Inc. Compositions and methods for polynucleotide sequencing
EP3556869A1 (en) 2013-11-26 2019-10-23 Illumina Inc. Compositions and methods for polynucleotide sequencing
US10364462B2 (en) 2013-11-26 2019-07-30 Illumina, Inc. Compositions and methods for polynucleotide sequencing
US11041196B2 (en) 2013-11-26 2021-06-22 Illumina, Inc. Compositions and methods for polynucleotide sequencing
US9689033B2 (en) 2013-11-26 2017-06-27 Illumina, Inc. Compositions and methods for polynucleotide sequencing
WO2015081178A1 (en) 2013-11-26 2015-06-04 Illumina, Inc. Compositions and methods for polynucleotide sequencing
EP2886663A1 (en) 2013-12-19 2015-06-24 Centre National de la Recherche Scientifique (CNRS) Nanopore sequencing using replicative polymerases and helicases
US11725235B2 (en) 2014-01-22 2023-08-15 Oxford Nanopore Technologies Plc Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10392658B2 (en) 2014-01-22 2019-08-27 Oxford Nanopore Technologies Ltd. Method for controlling the movement of a polynucleotide through a transmembrane pore
WO2015110813A1 (en) 2014-01-22 2015-07-30 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US11542551B2 (en) 2014-02-21 2023-01-03 Oxford Nanopore Technologies Plc Sample preparation method
US10669578B2 (en) 2014-02-21 2020-06-02 Oxford Nanopore Technologies Ltd. Sample preparation method
WO2015150787A1 (en) 2014-04-04 2015-10-08 Oxford Nanopore Technologies Limited Method of target molecule characterisation using a molecular pore
US10774378B2 (en) 2014-04-04 2020-09-15 Oxford Nanopore Technologies Ltd. Method of target molecule characterisation using a molecular pore
US11236385B2 (en) 2014-04-04 2022-02-01 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
US11649490B2 (en) 2014-04-04 2023-05-16 Oxford Nanopore Technologies Plc Method of target molecule characterisation using a molecular pore
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10167503B2 (en) 2014-05-02 2019-01-01 Oxford Nanopore Technologies Ltd. Mutant pores
US10844432B2 (en) 2014-05-02 2020-11-24 Oxford Nanopore Technologies, Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US11739377B2 (en) 2014-05-02 2023-08-29 Oxford Nanopore Technologies Plc Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
EP3702780A1 (en) 2014-07-31 2020-09-02 Illumina, Inc. Hybrid nanopore sensors
EP3982121A1 (en) 2014-07-31 2022-04-13 Illumina, Inc. Hybrid nanopore sensors
US10519499B2 (en) 2014-07-31 2019-12-31 Illumina, Inc. Hybrid nanopore sensors
US11732301B2 (en) 2014-07-31 2023-08-22 Illumina, Inc. Hybrid nanopore sensors
EP4379373A2 (en) 2014-07-31 2024-06-05 Illumina, Inc. Nucleic acid sequencing with hybrid nanopore sensors
US10961576B2 (en) 2014-07-31 2021-03-30 Illumina, Inc. Hybrid nanopore sensors
US11034734B2 (en) 2014-09-01 2021-06-15 Oxford Nanopore Technologies Ltd. Mutant pores
US12275761B2 (en) 2014-09-01 2025-04-15 Vib Vzw Mutant CsgG pores
EP4053150A1 (en) 2014-09-01 2022-09-07 Vib Vzw Mutant csgg pores
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
EP4108679A1 (en) 2014-10-07 2022-12-28 Oxford Nanopore Technologies plc Modified enzymes
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
US11965183B2 (en) 2014-10-07 2024-04-23 Oxford Nanopore Technologies Plc Modified enzymes
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
WO2016055777A2 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Modified enzymes
US11180741B2 (en) 2014-10-07 2021-11-23 Oxford Nanopore Technologies Ltd. Modified enzymes
US10570440B2 (en) 2014-10-14 2020-02-25 Oxford Nanopore Technologies Ltd. Method for modifying a template double stranded polynucleotide using a MuA transposase
WO2016059363A1 (en) 2014-10-14 2016-04-21 Oxford Nanopore Technologies Limited Method
US11390904B2 (en) 2014-10-14 2022-07-19 Oxford Nanopore Technologies Plc Nanopore-based method and double stranded nucleic acid construct therefor
US10689697B2 (en) 2014-10-16 2020-06-23 Oxford Nanopore Technologies Ltd. Analysis of a polymer
US11401549B2 (en) 2014-10-16 2022-08-02 Oxford Nanopore Technologies Plc Analysis of a polymer
US11613771B2 (en) 2014-10-17 2023-03-28 Oxford Nanopore Technologies Plc Methods for delivering an analyte to transmembrane pores
US10549274B2 (en) 2014-10-17 2020-02-04 Oxford Nanopore Technologies Ltd. Electrical device with detachable components
US10760114B2 (en) 2014-10-17 2020-09-01 Oxford Nanopore Technologies Ltd. Methods for delivering an analyte to transmembrane pores
WO2016059375A1 (en) 2014-10-17 2016-04-21 Oxford Nanopore Technologies Limited Methods for delivering an analyte to transmembrane pores
WO2016132124A1 (en) 2015-02-19 2016-08-25 Oxford Nanopore Technologies Limited Method
EP4019535A1 (en) 2015-02-19 2022-06-29 Oxford Nanopore Technologies plc Hetero-pores
WO2016132123A1 (en) 2015-02-19 2016-08-25 Oxford Nanopore Technologies Limited Hetero-pores
US10472673B2 (en) 2015-02-19 2019-11-12 Oxford Nanopore Technologies Ltd. Hetero-pores
US11307192B2 (en) 2015-02-19 2022-04-19 Oxford Nanopore Technologies Plc Method for producing a hetero-oligomeric pore comprising two different monomers in a specific stiochiometric ratio
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
WO2017098322A1 (en) 2015-12-08 2017-06-15 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
US10976300B2 (en) 2015-12-08 2021-04-13 Katholieke Universiteit Leuven Modified nanopores, compositions comprising the same, and uses thereof
EP4015531A2 (en) 2016-03-02 2022-06-22 Oxford Nanopore Technologies PLC Mutant pore
WO2017149317A1 (en) 2016-03-02 2017-09-08 Oxford Nanopore Technologies Limited Mutant pore
US11186868B2 (en) 2016-03-02 2021-11-30 Oxford Nanopore Technologies Plc Mutant pore
US12227800B2 (en) 2016-03-02 2025-02-18 Oxford Nanopore Technologies Plc Mutant pores
EP4019542A1 (en) 2016-03-02 2022-06-29 Oxford Nanopore Technologies plc Mutant pores
WO2017149316A1 (en) 2016-03-02 2017-09-08 Oxford Nanopore Technologies Limited Mutant pore
EP4019543A1 (en) 2016-03-02 2022-06-29 Oxford Nanopore Technologies plc Mutant pore
WO2017149318A1 (en) 2016-03-02 2017-09-08 Oxford Nanopore Technologies Limited Mutant pores
US12018326B2 (en) 2016-03-02 2024-06-25 Oxford Nanopore Technologies Plc Mutant pore
US11597970B2 (en) 2016-03-02 2023-03-07 Oxford Nanopore Technologies Plc Mutant pores
US10995372B2 (en) 2016-03-02 2021-05-04 Oxford Nanopore Technologies Ltd. Mutant pores
US11685949B2 (en) 2016-03-02 2023-06-27 Oxford Nanopore Technologies Plc Mutant pore
US10975428B2 (en) 2016-03-02 2021-04-13 Oxford Nanopore Technologies Ltd. Mutant pore
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
US12371458B2 (en) 2016-04-06 2025-07-29 Oxford Nanopore Technologies Plc Mutant pore
US11104709B2 (en) 2016-04-06 2021-08-31 Oxford Nanopore Technologies Ltd. Mutant pore
US11939359B2 (en) 2016-04-06 2024-03-26 Oxford Nanopore Technologies Plc Mutant pore
EP4122949A1 (en) 2016-04-06 2023-01-25 Oxford Nanopore Technologies plc Mutant pore
EP4397970A2 (en) 2016-04-06 2024-07-10 Oxford Nanopore Technologies PLC Mutant pore
US11649480B2 (en) 2016-05-25 2023-05-16 Oxford Nanopore Technologies Plc Method for modifying a template double stranded polynucleotide
WO2017203269A1 (en) 2016-05-25 2017-11-30 Oxford Nanopore Technologies Limited Method of nanopore sequencing of concatenaded nucleic acids
US12215384B2 (en) 2016-05-25 2025-02-04 Oxford Nanopore Technologies Plc Methods of characterising target polynucleotides
US11098355B2 (en) 2016-05-25 2021-08-24 Oxford Nanopore Technologies Ltd. Method of nanopore sequencing of concatenated nucleic acids
EP4063521A1 (en) 2016-05-25 2022-09-28 Oxford Nanopore Technologies PLC Method of nanopore sequencing
US11596940B2 (en) 2016-07-06 2023-03-07 Oxford Nanopore Technologies Plc Microfluidic device
WO2018060740A1 (en) 2016-09-29 2018-04-05 Oxford Nanopore Technologies Limited Method for nucleic acid detection by guiding through a nanopore
US11739379B2 (en) 2016-09-29 2023-08-29 Oxford Nanopore Technologies Plc Method for nucleic acid detection by guiding through a nanopore
US11085078B2 (en) 2016-09-29 2021-08-10 Oxford Nanopore Technologies Limited Method for nucleic acid detection by guiding through a nanopore
US11390910B2 (en) 2016-10-21 2022-07-19 Oxford Nanopore Technologies Plc Method
WO2018073604A1 (en) 2016-10-21 2018-04-26 Oxford Nanopore Technologies Limited Method
EP4269617A2 (en) 2016-12-01 2023-11-01 Oxford Nanopore Technologies plc Methods and systems for characterizing analytes using nanopores
US11466317B2 (en) 2016-12-01 2022-10-11 Oxford Nanopore Technologies Plc Methods and systems for characterizing analytes using nanopores
WO2018100370A1 (en) 2016-12-01 2018-06-07 Oxford Nanopore Technologies Limited Methods and systems for characterizing analytes using nanopores
US11840556B2 (en) 2017-02-10 2023-12-12 Oxford Nanopore Technologies Plc Modified nanopores, compositions comprising the same, and uses thereof
WO2018146491A1 (en) 2017-02-10 2018-08-16 Oxford Nanopore Technologies Limited Modified nanopores, compositions comprising the same, and uses thereof
US11789014B2 (en) 2017-05-04 2023-10-17 Oxford Nanopore Technologies Plc Method of determining the presence or absence of a target analyte comprising using a reporter polynucleotide and a transmembrane pore
WO2018211241A1 (en) 2017-05-04 2018-11-22 Oxford Nanopore Technologies Limited Transmembrane pore consisting of two csgg pores
WO2018203071A1 (en) 2017-05-04 2018-11-08 Oxford Nanopore Technologies Limited Method of determining the presence or absence of a target analyte comprising using a reporter polynucleotide and a transmembrane pore
EP3892627A1 (en) 2017-05-04 2021-10-13 Oxford Nanopore Technologies Limited Transmembrane pore consisting of two csgg pores
US12024541B2 (en) 2017-05-04 2024-07-02 Oxford Nanopore Technologies Plc Transmembrane pore consisting of two CsgG pores
US11945840B2 (en) 2017-06-30 2024-04-02 Vib Vzw Protein pores
US11572387B2 (en) 2017-06-30 2023-02-07 Vib Vzw Protein pores
US12084477B2 (en) 2017-06-30 2024-09-10 Vib Vzw Protein pores
CN111225744A (zh) * 2017-10-23 2020-06-02 豪夫迈·罗氏有限公司 使用渗透不平衡除去和重新插入膜中的蛋白纳米孔
WO2019081442A1 (en) * 2017-10-23 2019-05-02 F. Hoffmann-La Roche Ag REMOVAL AND REINTEGRATION OF PROTEIN NANOPORES IN A MEMBRANE USING AN OSMOTIC IMBALANCE
EP4303317A3 (en) * 2017-10-23 2024-04-24 F. Hoffmann-La Roche AG Removing and reinserting protein nanopores in a membrane using osmotic imbalance
US12226766B2 (en) 2017-10-23 2025-02-18 Roche Sequencing Solutions, Inc. Removing and reinserting protein nanopores in a membrane using osmotic imbalance
US12121894B2 (en) 2017-11-29 2024-10-22 Oxford Nanopore Technologies Plc Microfluidic device
US12195777B2 (en) 2018-02-02 2025-01-14 Oxford Nanopore Technologies Plc Polynucleotide synthesis method, kit and system
US11725205B2 (en) 2018-05-14 2023-08-15 Oxford Nanopore Technologies Plc Methods and polynucleotides for amplifying a target polynucleotide
WO2019224558A1 (en) 2018-05-24 2019-11-28 Oxford Nanopore Technologies Limited Method for selecting polynucleotides based on the size in a globular form
WO2019234432A1 (en) 2018-06-06 2019-12-12 Oxford Nanopore Technologies Limited Method
US11920193B2 (en) 2018-06-06 2024-03-05 Oxford Nanopore Technologies Plc Method of characterizing a polynucleotide
WO2020016573A1 (en) 2018-07-16 2020-01-23 Oxford University Innovation Limited Molecular hopper
US12247990B2 (en) 2018-07-16 2025-03-11 Oxford University Innovation Limited Molecular hopper
EP4488285A2 (en) 2018-07-16 2025-01-08 Oxford University Innovation Limited Molecular hopper
WO2020025909A1 (en) 2018-07-30 2020-02-06 Oxford University Innovation Limited Assemblies
GB201818216D0 (en) 2018-11-08 2018-12-26 Oxford Nanopore Tech Ltd Pore
WO2020095052A1 (en) 2018-11-08 2020-05-14 Oxford Nanopore Technologies Limited Pore
WO2020128517A1 (en) 2018-12-21 2020-06-25 Oxford Nanopore Technologies Limited Method of encoding data on a polynucleotide strand
EP4636767A2 (en) 2018-12-21 2025-10-22 Oxford Nanopore Technologies PLC Method of encoding data on a polynucleotide strand
US11789006B2 (en) 2019-03-12 2023-10-17 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
US12392766B2 (en) 2019-03-12 2025-08-19 Oxford Nanopore Technologies Plc Nanopore sensing device, components and method of operation
EP4607190A2 (en) 2019-03-12 2025-08-27 Oxford Nanopore Technologies PLC Nanopore sensing device and method
WO2020183172A1 (en) 2019-03-12 2020-09-17 Oxford Nanopore Technologies Inc. Nanopore sensing device and methods of operation and of forming it
US11994486B2 (en) 2019-03-19 2024-05-28 Oxford Nanopore Technologies Plc Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
WO2020188235A1 (en) 2019-03-19 2020-09-24 Oxford Nanopore Technologies Limited Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
US12235233B2 (en) 2019-03-19 2025-02-25 Oxford Nanopore Technologies Plc Current measurement apparatus, molecular entity sensing apparatus, method of measuring a current, method of sensing a molecular entity
WO2020208357A1 (en) 2019-04-09 2020-10-15 Oxford Nanopore Technologies Limited Pore
GB201907246D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
WO2020234612A1 (en) 2019-05-22 2020-11-26 Oxford Nanopore Technologies Limited Method
GB201907244D0 (en) 2019-05-22 2019-07-03 Oxford Nanopore Tech Ltd Method
WO2021058975A1 (en) 2019-09-27 2021-04-01 Oxford Nanopore Technologies Limited Methods and systems for preparing a nucleic acid construct for single molecule characterisation
GB201913997D0 (en) 2019-09-27 2019-11-13 Oxford Nanopore Tech Ltd Method
WO2021078971A2 (en) 2019-10-25 2021-04-29 Oxford Nanopore Technologies Limited Improved nanopore sensing device, components and method of manufacture
GB201917060D0 (en) 2019-11-22 2020-01-08 Oxford Nanopore Tech Ltd Method
WO2021099801A1 (en) 2019-11-22 2021-05-27 Oxford Nanopore Technologies Limited Method for double strand sequencing
EP4270008A2 (en) 2019-12-02 2023-11-01 Oxford Nanopore Technologies PLC Method of characterising a target polypeptide using a nanopore
WO2021111125A1 (en) 2019-12-02 2021-06-10 Oxford Nanopore Technologies Limited Method of characterising a target polypeptide using a nanopore
WO2021111139A1 (en) 2019-12-04 2021-06-10 Oxford Nanopore Technologies Limited Method
WO2021198695A1 (en) 2020-04-03 2021-10-07 King's College London Method of detecting an analyte in a medium comprising a light scattering constituent
GB202004944D0 (en) 2020-04-03 2020-05-20 King S College London Method
WO2021255414A1 (en) 2020-06-17 2021-12-23 Oxford Nanopore Technologies Limited Nanopore support structure and manufacture thereof
WO2021255475A1 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited A method of selectively characterising a polynucleotide using a detector
WO2021255476A2 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited Method
WO2021255477A1 (en) 2020-06-18 2021-12-23 Oxford Nanopore Technologies Limited Method of repeatedly moving a double-stranded polynucleotide through a nanopore
US12411125B2 (en) 2020-07-17 2025-09-09 Oxford Nanopore Technologies Plc Nanopore sensing device
WO2022013551A1 (en) 2020-07-17 2022-01-20 Oxford Nanopore Technologies Limited Nanopore sensing device
WO2022074397A1 (en) 2020-10-08 2022-04-14 Oxford Nanopore Technologies Limited Modification of a nanopore forming protein oligomer
GB202107192D0 (en) 2021-05-19 2021-06-30 Oxford Nanopore Tech Ltd Method
WO2022243692A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Methods for complement strand sequencing
WO2022243691A1 (en) 2021-05-19 2022-11-24 Oxford Nanopore Technologies Plc Multiplex methods of detecting molecules using nanopores
GB202107354D0 (en) 2021-05-24 2021-07-07 Oxford Nanopore Tech Ltd Method
WO2023026056A1 (en) 2021-08-26 2023-03-02 Oxford Nanopore Technologies Plc Nanopore
WO2023118892A1 (en) 2021-12-23 2023-06-29 Oxford Nanopore Technologies Plc Method
WO2023118404A1 (en) 2021-12-23 2023-06-29 Oxford Nanopore Technologies Plc Pore
WO2023118891A1 (en) 2021-12-23 2023-06-29 Oxford Nanopore Technologies Plc Method of characterising polypeptides using a nanopore
WO2023194713A1 (en) 2022-04-04 2023-10-12 Oxford Nanopore Technologies Plc Method
GB202204919D0 (en) 2022-04-04 2022-05-18 Oxford Nanopore Tech Plc Method
WO2023198911A2 (en) 2022-04-14 2023-10-19 Oxford Nanopore Technologies Plc Novel modified protein pores and enzymes
WO2023222657A1 (en) 2022-05-17 2023-11-23 Oxford Nanopore Technologies Plc Method and adaptors
US12503729B2 (en) 2022-05-31 2025-12-23 Oxford Nanopore Technologies Plc Enzyme method
WO2024033421A2 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024033422A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024033447A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc De novo pores
WO2024033443A1 (en) 2022-08-09 2024-02-15 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024089270A2 (en) 2022-10-28 2024-05-02 Oxford Nanopore Technologies Plc Pore monomers and pores
WO2024094986A1 (en) 2022-10-31 2024-05-10 Oxford Nanopore Technologies Plc Method
WO2024100270A1 (en) 2022-11-11 2024-05-16 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024165853A1 (en) 2023-02-07 2024-08-15 Oxford University Innovation Limited Method of characterising a peptide, polypeptide or protein using a nanopore
WO2024200280A1 (en) 2023-03-24 2024-10-03 Oxford Nanopore Technologies Plc Method and kits
WO2024213802A1 (en) 2023-04-14 2024-10-17 Oxford Nanopore Technologies Plc Novel pore monomers and pores
WO2024235919A1 (en) 2023-05-12 2024-11-21 Oxford Nanopore Technologies Plc Modified helicases
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
WO2024236325A1 (en) 2023-05-18 2024-11-21 Oxford Nanopore Technologies Plc Method and products for characterizing a polynucleotide using a nanopore
WO2025040887A1 (en) 2023-08-18 2025-02-27 Oxford University Innovation Limited Nanopore
WO2025046221A1 (en) 2023-08-25 2025-03-06 Oxford University Innovation Limited Power unit comprising distinct droplets separated by an amphiphilic membrane
WO2025083247A1 (en) 2023-10-18 2025-04-24 Oxford Nanopore Technologies Plc Novel enzymes
WO2025099094A1 (en) 2023-11-06 2025-05-15 Oxford Nanopore Technologies Plc Method
WO2025228949A1 (en) 2024-04-29 2025-11-06 Oxford Nanopore Technologies Plc Photo-active conjugates and their uses
WO2025238370A1 (en) 2024-05-17 2025-11-20 Oxford Nanopore Technologies Plc Enhanced polynucleotide sequencing
GB202407228D0 (en) 2024-05-21 2024-07-03 Oxford Nanopore Tech Plc Method
WO2025242713A1 (en) 2024-05-21 2025-11-27 Oxford Nanopore Technologies Plc A method for sequencing chromatin

Also Published As

Publication number Publication date
GB0505971D0 (en) 2005-04-27
US7939270B2 (en) 2011-05-10
JP2008537106A (ja) 2008-09-11
WO2006100484A3 (en) 2008-05-29
JP5253148B2 (ja) 2013-07-31
EP1861071B1 (en) 2010-07-07
DE602006015292D1 (de) 2010-08-19
ATE473438T1 (de) 2010-07-15
US20080153150A1 (en) 2008-06-26
EP1861071A2 (en) 2007-12-05

Similar Documents

Publication Publication Date Title
US7939270B2 (en) Delivery of molecules to a lipid bilayer
US12186755B2 (en) Multi-well separation apparatus and reagent delivery device
Fertig et al. Activity of single ion channel proteins detected with a planar microstructure
US20190242913A1 (en) Lipid bilayer sensor system
EP3139154A1 (en) Spectroscopy sensor and method for manufacturing same
WO2003093791A2 (en) Fast electrical lysis of cells and rapid collection of the contents thereof using capillary electrophoresis
JP2007529203A (ja) 統合細胞操作及び測定方法及び装置
JP2005528102A (ja) 多用途の生存マイクロアレイを用いる、細胞に基づく高処理量アッセイのための方法
Holden et al. Direct transfer of membrane proteins from bacteria to planar bilayers for rapid screening by single-channel recording
WO2016168301A1 (en) Compositions and methods for disaggregation of biological tissue samples
JP2015221034A (ja) 細胞分裂を観察するデバイスおよび方法
US20150024429A1 (en) Method and device for the homogeneous distribution of suspended cell components
JP2012517234A5 (enExample)
US20110300618A1 (en) Semi-automated device for single parameter and multi-parameter phenotyping of cells
CN116438439A (zh) 利用粒子运动的生物传感器
WO2020175526A1 (ja) 微小構造体、その作製方法およびそれを利用した分子検出方法
EP1591783B1 (en) Method for detection and analysis of macromolecular complexes
Liu et al. Addressing Challenges in Fluid Flow-Induced Cell Membrane Oscillation by Nanopipettes
EP3770600A1 (en) Purification process for cells
HK40057195A (en) Method for seeding cells on a sensor surface
JPH0755664A (ja) 細胞及び染色体の標本用基材
WO2022082025A1 (en) Cellular response analysis method
Lübben et al. On-Line Monitoring of Protein and Susbtrate/Product Concentration in Mammalian Cell Cultivation Process
Patil et al. Automated Reactor For Extraction And Manipulation Of Sub-Megabase Fragments Of Genomic DNA With Flow-Focusing

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 2006726479

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2008502471

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

NENP Non-entry into the national phase

Ref country code: RU

WWW Wipo information: withdrawn in national office

Country of ref document: RU

WWE Wipo information: entry into national phase

Ref document number: 11884927

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2006726479

Country of ref document: EP