WO2005093042A1

WO2005093042A1 - Novel thermophilic bacterium and soil blend containing the same

Info

Publication number: WO2005093042A1
Application number: PCT/JP2005/001938
Authority: WO
Inventors: Naohiko Sato; Keiko Okamoto; Ryouma Mike
Original assignee: B & L Corporation
Priority date: 2004-03-25
Filing date: 2005-02-09
Publication date: 2005-10-06
Also published as: JPWO2005093042A1; JP3905553B2; TW200602485A

Abstract

It is intended to provide a novel thermophilic bacterium which is isolated and identified from a stevia herb and has been deposited under the deposition number FERM P-20039; a compost containing this thermophilic bacterium at a concentration of 1×106 cfu/g or above which is produced by using the thermophilic bacterium; and a soil blend for growing plants which contains this thermophilic bacterium at a high concentration of 1×105 cfu/g or above in a material useful in soil for agricultural use. By growing field crops in soil containing the above thermophilic bacterium, the yield can be elevated and, at the same time, residual pesticides and nitrate-nitrogen contained in the crops can be reduced, thereby providing crops that can be taken without worries.

Description

Specification

New thermophile and soil compound containing it

Technical field

[0001] The present invention is obtained from a Stevia plant, belongs to Ureibacillus thermosphaericus, and has an address: 305-8566 Tsukuba, Ibaraki Prefecture 1-1 1-1 Central No. 6, Name: Independent Administration Accession No. FERM P-20039 on April 30, 2004 at the National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary, a new thermophile, a compost obtained by culturing it, It relates to a loam compound containing a large amount of fungi. The present invention relates to a method for cultivating agricultural products that improves fertilizer absorption of plants, prevents disease and insect damage, and reduces residual pesticides in agricultural products by applying the compost or soil compounding material of the present invention.

Background art

[0002] Recently, as a result of increasing the yield of agricultural products by using a large amount of chemical fertilizers, there is a tendency that the soil is thin and the basic physical strength of plants that grow is reduced. As a result, crops are vulnerable to pests and insects, and the use of pesticides such as powerful insecticides and fungicides gradually increases, and these pesticides are transferred to agricultural products as residual pesticides. Pesticide residues ingested into the human body along with agricultural products accumulate in the human body and are considered to be a cause of irritability and other modern diseases. Even if a large amount of chemical fertilizer is applied for the purpose of large-scale harvesting, the amount of nutrients absorbed by plants is limited and the soil is over-nutrient.

[0003] Recently, pesticide-free cultivation and reduced pesticide cultivation have been performed. However, as a real problem, there was a phenomenon that even after switching to the pesticide-free farming method, the pesticides used in the soil remained in the soil and were absorbed by the crops and the pesticides were detected in the crops. In addition, even if an attempt is made to switch to pesticide-free, if crops are attacked by pests, pesticides must be used, making it difficult to obtain agricultural products free of residual pesticides.

For example, Patent Document 1 describes a technique for applying a powder of a stevia plant to soil to maintain healthy bacterial flora in the soil and reduce the amount of pesticides used. Patent Document 2 discloses that the internal temperature is controlled by mixing powder of stevia plant with compost raw material. It is described that the temperature reaches around 80 ° C and the production of compost is accelerated.

[0004] On the other hand, nitrogen contained in nitrate is an important nutrient of plants and an essential element for growth. Especially in vegetables, most of the nitrogen is absorbed as nitrate ions. The absorbed nitric acid is reduced in the plant to become amino acids, proteins, nucleic acids, etc., and when ingested by the human body, is decomposed into amino acids and resynthesized into proteins. Since nitric acid is reduced and assimilated into amino acids in the plant and is used for the first time, if it is absorbed more, it is accumulated as unreduced nitrate.

[0005] In recent years, it has been revealed that excessive intake of nitrate groups has an adverse effect on the human body. This is thought to be the result of the nitrate group binding to the amino group of the protein to form ditrosamine. It is therefore desirable to keep nitric acid intake as low as possible. Since vegetables are the main source of nitrate, vegetables with low nitrate content are required. Although low nitridation is being studied by adjusting fertilizers, reducing the amount of fertilizer leads to a decrease in yield and deterioration in quality.

Patent Document 1: JP-A-2000-53517

Patent Document 2: Japanese Patent Application Laid-Open No. 2002-60290

Disclosure of the invention

Problems to be solved by the invention

[0006] The present invention relates to the powdery power of Stevia plants, which has the effect of increasing the internal temperature to around 80 ° C in the compost production process and has the effect of maintaining the plants healthy. We tried to isolate and identify the bacteria. The purpose of the present invention is to provide a technique for increasing the fertilizer absorption capacity of the plant and improving the quality by growing this fungus and applying it to the plant, and for reducing the residual pesticides and nitrate nitrogen in agricultural products such as vegetables. I do.

Means for solving the problem

[0007] The present invention achieves the above-mentioned object, and its composition belongs to "Reybacillus" thermosphaericus (Ureibacillus thermosphaericus), and has an address: 305-8566 Tsukuba, Ibaraki Prefecture 1-1-1-1 Chuo 6.Name: The new thermophile deposited as Accession No. FERM P-20039 at the National Institute of Advanced Industrial Science and Technology (AIST) at the Patent Organism Depositary Research Center on April 30, 2004. To increase the quality of compost produced by spraying This is a method of cultivating agricultural products, which comprises cultivating and applying a soil conditioner containing at least 1 × 10 ⁵ cfo / g to soil where agricultural plants grow.

[0008] That is, in the present invention, stevia powder lg is diluted by a serial dilution method, 0.1 ml of each serial dilution is inoculated on 0.5% meat extract power, Liptosoy agar medium, and cultured at 55 ° C for 2 days. Thermophilic bacteria were isolated. Furthermore, the separation culture was repeated in the same medium, and four single bacteria were separated. On the other hand, in the compost obtained by adding a small amount of powder of Stevia plant and fermenting, only one of the above simple bacteria was present. This single bacterium was identified by 16S rDNA nucleotide sequence analysis. As a result of 16S rDNA partial base sequence analysis (598 base), it was found that 596Z598 salt was consistent with ゥ Leybacillus thermosphaericus (Ureibacillus thermosphaericus), and that it belonged to ゥ Leybacillus' thermosphaericus (99% probability). This thermophilic bacterium had high resolution for various proteins, and not only proliferated in the presence of high concentrations of various organic pesticides, but also exhibited higher proliferative ability in the presence of high concentrations of pesticides. It also has the ability to reduce nitrate groups to nitrite groups and further to ammonium groups.

The invention's effect

[0009] When the thermophilic bacterium of the present invention is blended into a compost raw material, a high-quality compost containing a large amount of potassium ions and orthophosphate ions can be obtained. If this compost is applied to the soil where the crops are grown, a bacterial flora favorable for the growth of a unique plant, mainly the thermophilic bacterium of the present invention, is formed, and the quality and yield of agricultural products are improved. Even in soils where a large amount of pesticide residues remain, and even if some pesticide is used, no pesticides are detected in the harvested crops and nitrate nitrogen is reduced. Therefore, according to the present invention, it has become possible to provide a large amount of agricultural products that are safe for the human body by simple means.

Brief Description of Drawings

FIG. 1 is a graph showing the relative growth rate of the thermophilic bacterium of the present invention with respect to carbamate pesticides.

FIG. 2 is a graph showing the relative growth rate of the thermophilic bacterium of the present invention with respect to organophosphorus pesticides.

FIG. 3 is a graph showing the relative growth rate of the thermophilic bacterium of the present invention with respect to pyrethroid pesticides. It is.

FIG. 4 is a graph showing the nitrate-reducing properties of the thermophilic bacterium of the present invention together with the number of culture days. BEST MODE FOR CARRYING OUT THE INVENTION

[0011] The novel thermophilic bacterium of the present invention is located at Tsukuba Ibaraki, Higashi 1-11, Chuo No. 6, 305-8566, Japan, Name: National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary! It is a spore-forming bacterium deposited on April 30, 2004 under accession number FERM P-20039. This bacterium showed no growth on tryptosome agar medium. When 0.5% meatex is added to this medium, 3+ growth is observed, and 3+ growth is also observed in 1% casein-added agar medium, and the ability to degrade proteins and polypeptides such as meat extract and casein is improved. It turned out to be high.

The staining and biochemical properties of this bacterium are bacilli, and are gram-negative, catalase-positive, oxidase-positive, motile, and spore-staining. The growth activity of each bacterium at each temperature was reduced to ¾kl.

[0012] [Table 1]

“Ten” indicates that the cells can proliferate, and the numerical value indicates the size of the colony.

It shows maximum growth at 60 ° C and cannot grow at 70 ° C.

Table 2 shows the substrate resolution of the thermophilic bacterium of the present invention.

[0013] [Table 2] Medium species propagation

Tributisoy agar medium ―

0.5% meat extract with Tribute soy agar 3 +

Cookmeat medium ―

1% Casein Agar Medium 30

Tributsoy agar with 0.5% gelatin ―

2% yolk-supplemented tributisoto agar-

1% soluble starch added Tribute soy bouillon

1% soluble starch-added soy agar medium weak

[0014] The novel thermophilic bacterium of the present invention is located at Tsukuba Ibaraki, Higashi, 305-8566, Japan, 1st center, No. 6, Name: National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary Center! On April 30, 2004, it was deposited under accession number FERM P-20039. The partial nucleotide sequence by 16S rDNA nucleotide sequence analysis was as shown in the sequence listing. According to this partial nucleotide sequence, all 596/598 bases of the thermophilic bacterium of the present invention are identical, and 99%

The homology was found to be high with Ureibacillus thermosphaericus.

[0015] Stevia powder as a raw material of the present invention is a perennial plant of the chrysanthemum family, Stevia rebaudiana bertoni (Stevia rebaudiana bertoni) and a similar plant, which are native to South America. The cuttings, leaves and stems are dried and pulverized. Commercially available is a mixture of leaves and stems. In the present invention, this powder is mixed with a compost material, fermented, separated from the resulting compost, identified, and its physiological characteristics are clarified.

[0016] The compost raw materials include, but are not particularly limited to, cow dung, pig dung, chicken dung, activated sludge, vegetable waste, straw waste, food factory waste, pharmaceutical factory waste, household waste, and the like. Raw materials to be contained are preferred. When compost is made by adding about 0.01-1.5% by mass of powder of Stevia plant to this compost raw material, the odor peculiar to the compost raw material disappears in one day, and the internal temperature naturally rises due to fermentation. Rises to over 80 ° C. Conventionally, the upper limit of fermentation temperature in compost is at most 65-70 ° C even if EM bacteria are used, and high temperatures of 80 ° C could not be obtained without external force heating. Composting time was also reduced, and the resulting compost was of good quality and touched by hand The feeling is soft as a moisturizer.

[0017] As a result of composting, the address of the present invention relating to the type of compost raw material is as follows: τ 305-8566 Tsukuba, Higashi, Ibaraki 1-1-1 Central No. 6, Name: National Institute of Advanced Industrial Science and Technology, Patent Organism A new calf belonging to Ureibacillus thermosphaericus, which was deposited at the Depositary Center on April 30, 2004 as FERM P-20039. The concentration of the substance is about 10 × 10 ⁷ cfo / g. It is contained in.

Therefore, if the compost obtained by the present invention is further blended with a new compost material, a new compost containing a large amount of the thermophilic bacterium of the present invention can be obtained using a smaller amount of the inoculum.

The present invention is a compost containing the thermophilic bacterium at 1 × 10 ⁶ clu / g or more, preferably 1 × 10 ′ cfo / g or more. Alternatively, a soil compounding material containing the thermophilic bacterium of the present invention in an amount of 1 × 10 ⁵ cfo / g or more, preferably 1 × 10 ⁶ clu / g or more, in a material useful for agricultural soil. Materials useful for agricultural soil include compost, rice husk, rice straw, bran, dried plants, legumes such as lotus, etc. It is a material that has the effect of improving air permeability, improving rooting, and the like. Among these, pressing and cutting of dried Stevia plant powder and coconut pulp are preferred. The present invention is a soil compounded material containing the thermophilic bacterium of the present invention at 1 × 10 ⁵ clu / g or more, preferably 1 × 10 ⁶ clu / g or more, in a material useful for these agricultural soils.

In order to manufacture the thermophilic bacterium of the present invention, the thermophilic bacterium culture of the present invention, which is separately cultured and propagated, may be mixed with a material useful for agricultural soil. The thermophilic bacterium of the present invention may be composted to be separately cultured and propagated, or the thermophilic bacterium of the present invention may be cultured in a substrate containing a protein or polypeptide. When the soil compound of the present invention is added to soil for growing agricultural plants, the nutrient absorption of the plant is improved and the yield is increased, and thermophilic bacteria are used even in soil where pesticides previously used remain. Proliferate and do not leave pesticide residues in agricultural products. When the soil conditioner contains less than 1 × 10 ⁵ cfo / g of the thermophilic bacterium of the present invention, the effect of the present invention is not sufficiently exhibited. When measuring the content of the thermophilic bacterium of the present invention, it is preferable to culture and measure at 55 ° C to 60 ° C. [0020] The pesticides removed by the bacterium of the present invention include dichlorvos, EPN, edifenphos, etoprophos, etrimphos, quinonalephos, chlorpyrifos, chronorefenvinphos, thiomethone, dimethylvinphos, dimethoace, tolclophosmethyl, Diazin, terbufos, parathion, parathion-methyl, pyraclophos, pirimiphos-methyl, fenitrothion, fenthion, fentoate, butamiphos, protiphos, hosalon, isoxathion, ethione, chlorpyrifos-methyl, salicion, cyanophos, diclofenthion, disulfoton, malathion, etc. Can be mentioned.

[0021] Furthermore, a-BHC, β-BHC, γ-BHC, δ-BHC, pp, one DDD, pp, one DDE, pp, -DDT, op'-DDT, aldrin, endrin, dieldrin, dicohol, terftrin , Chlorinated pesticides such as fen block, chlordane and the like. Examples of the captan agrochemicals include captahole, captan, chlorbenzylate and the like. Examples of carbamate pesticides include lannate, algicable, ethiofen potable, oxamil, potash rubalin, pirimicarb, fenobucalp, bendiocalp, esprocalp, ventiocarb and the like.

[0022] Others, pyrethroid pesticides such as cyhalothrin, cyfluthrin, cypermethrin, tefluthrin, deltamethrin, tralomethrin, fenvalerate, fenpropatrin, flucitrinate, fluvalinate, permethrin; amitraz, clofentezin, tebufenvirad, pyrimethrin Acaricides such as daben; fungicides such as oxadixyl, diphenoconazole, tricyclazole, bitertanol, phenalmon, flutolanil, procymidone, probenazole, microbutanil, metalaxyl, mepromil; triazine pesticides such as simetryn and triazine; teurchlor And acid amide pesticides such as pretilachlor, mefenacet and the like.

[0023] By applying the soil conditioner of the present invention to the soil, the bacterial flora in the soil can be obtained. Address: Tsukuba, Ibaraki Pref., 305-8566 1-1-1-1 Chuo No. 6, Name: National Institute of Advanced Industrial Science and Technology According to the Patent Depositary on April 30, 2004, the new thermophilic bacterium, which was deposited under accession number FERM P-20039, was placed in an advantageous state for plant growth, and was cultivated. Agricultural products reduce nitrate nitrogen content without reducing fertilizer application rate, grow well, and no residual pesticides are detected. Example 1

(1) Isolation of thermophilic bacteria

Commercially available stevia powder lg was diluted by the serial dilution method, and 0.1 ml of each serial dilution was inoculated into 0.5% meat extract 卟 Liptosoy agar medium, and cultured at 55 ° C for 2 days to separate thermophilic bacteria. . Further, the same separation culture in the same medium was repeated 20 times or more to obtain four single bacteria. The single bacterium obtained was identified by Ureibacillus

The commercially available stevia powder contained 6.3 × 10 ⁴ clu / g of the thermophilic bacterium of the present invention. Furthermore, identification by whole nucleotide sequence analysis revealed that Bacillus thermoamvlovorans (99%)-another first thermophile that killed 99%-and Bacillus thermoamvlovorans (Bacillus thermoamvlovorans).

Three new strains of thermoamvlovorans and 99% —other thermophiles that kill 99% —and another second thermophile that kill 99% —thermophilic bacteria were obtained. None of these three strains were found in compost produced by spontaneous heating above 80 ° C.

(2) Manufacture of compost

A concrete compost tank with an open top was used and divided into three rooms by concrete bulkheads. Drainage holes were provided on the bottom, and piping with fumaroles opened on the top at regular intervals was placed on the bottom of the compost tank to ensure air permeability.

10 tons of bedding containing cow manure and 10 tons of chicken manure were mixed. Since the mixture was in a sticky state, 15% by weight of rice hulls and hay were blended to eliminate the sticky state and ensure physical properties to ensure air permeability. Further, 100 kg of dry powder of Stevia plant was added and mixed well. This compost material is filled into the first chamber of the above compost tank, pressed to eliminate the fluffy state, and water is added to the material so that its material strength also oozes out. Condition.

[0026] On the day of the work, the odor peculiar to animal manure was full, but the odor disappeared one day later.

Two days later, the internal temperature reached 80 ° C, and three days later it reached 81 ° C. Six days later, the internal temperature reached 76 ° C, so the unit was turned back. Fourteen days later, the internal temperature dropped to 55 ° C, so the material was transferred to the first tank and the second tank. Within 30 days The temperature of the section was lowered to 50 ° C., and the material was transferred to the second tank and the third tank was turned substantially. After 43 days, they were turned over, and the ripe compost was obtained in less than three months. During the compost production process, water was supplied as much as possible to leach material. The ordinary compost emits an ammonia odor when water is added thereto, but the compost of this example emits a rather rich fragrance without any bad odor even when water is added.

The number of the thermophilic bacterium of the present invention in this compost was determined by culturing at 55 ° C. and found to be 1.37 × 10 ⁷ clu / g.

[0027] (3) Carbamate-based agricultural youth tolerance test

The resistance of the thermophilic bacterium of the present invention to a lannate 45 wettable powder (trade name) that eliminates aphids and the like as a carnomate pesticide was examined. The component of the lanthanate wettable powder used was mesomil {S-methyl-N [(methylcarbamoyl) oxythioacetimidate]} 45%, and it is usually used after diluting 1000-2000 times.

As a medium, a 0.5% meat extract power Liptosoy agar medium was used. The activated thermophilic bacterium of the present invention was inoculated with 2 platinum wires into 10 ml of 0.5% meat extract power Liptosoy agar medium from the slant medium, and pre-cultured at 60 ° C for 24 hours before use.

Inoculate 0.5% meat extract caribbean liquid medium supplemented with 0, 0.1, 1, 10, 100, lOOOOppm of runner wettable powder, and incubate at 60 ° C and grow 1 day and 2 days later The rate was measured. The growth rate of thermophilic bacteria was measured at OD660nm, and the relative growth rate is shown in FIG. The plot in Figure 1 is the average of three experiments.

[0028] (4) Organophosphorus agricultural resistance test

The resistance of the thermophilic bacterium of the present invention to Takeda Ortran solution (trade name) as an organophosphorus pesticide was examined. Ortran contains 15% acephate (O, S-dimethyl-N-acetylphosphoramido thioate), 85% organic solvent and surfactant. Usually 250-500 times [Use after diluting. Honoré Bok run solution to 0, 0.4, 4.0, 40, except for using the 400ppm added Caro was 0.5 ^_0/0 meat Ekisuka卟Ripusoi liquid medium, a test in the same manner as in the carbamate pesticides The relative growth rate was shown in FIG. The plot in Figure 2 is the average of three experiments.

(5) Pyrethroid Agricultural Resistance Test The resistance of the thermophilic bacterium of the present invention to Sankyo Trevo-Emulsion (trade name) as a pyrethroid pesticide was examined. Trevo-emulsion contains 20% of ethophene block [2- (4 ethoxyphenyl) 2 methyl propyl = 3 phenoxybenzyl = ether], an organic solvent and a surfactant 80%. It is usually used after diluting 1000-2000 times. Trepo-emulsion was inoculated in 0.5, 0.1, 1, 10, 100, 0.5% meat extract carotripsy liquid medium supplemented with lOOOOppm, and tested in the same manner as for carbamate pesticides. The relative growth rate is shown in FIG. The plot in Figure 3 is the average of three experiments.

From FIG. 1 to FIG. 3, it is presumed that the thermophilic bacterium of the present invention has a high resistance to pesticides and is rather assimilated, rather than vigorously.

[0030] (6) m ^ m

The thermophilic bacterium of the present invention was inoculated into a 0.1% ΚΝΟ-containing peptone aqueous medium, and incubated at 55 ° C for 5 days.

Three

The culture was continued for a while. Nitrite production was measured at OD 540 nm using a nitrite detection reagent consisting of α-naphthylamine and sulfanilic acid every 24 hours of culture, and the results are shown in FIG. Furthermore, after culturing in the same medium under the same conditions, the presence or absence of the ability to produce ammonium by the Nessler reagent was examined.

From the above results, it was found that the thermophilic bacterium of the present invention has high nitrate reduction performance. Example 2

[0031] An experiment was conducted on sweet potato, which was said to prefer lean land in ancient times. After the shrub cultivation was completed, the abandoned soil in Fukuoka Prefecture, which had been left for several years, was used. On May 18, each of the 3.5 m ² (lm X 3.5 m) stevia compost plot of the present invention, the cow dung compost plot and the untreated plot obtained using the same raw material as the compost of the present invention and without the addition of stevia powder were used. Three test plots were established. Each of the compost plots was sprayed at 4.4 kg / m ² . As a control, three experimental plots including an untreated plot were set up. 29 days after each fertilizer application (June 16), 13 sweet potato (Naruto Kintoki) seedlings were planted on each ridge at 25 cm intervals and harvested on October 25. After harvesting, washing with water and drying at room temperature for 1 day (October 26), the number and weight of 10 sweet potato seedlings of each size were measured and shown in Table 3. Was. The sugar content was measured using a Brix meter, and the results are shown in Table 3.

[Table 3] Yield sugar content

Number and weight (kg) (%) Cattle manure compost of the present invention 27.1 ± 2.5 27.30 ± 0.47 1.20 Conventional cow manure compost 13.9 ± 3.5 6 4 2 2 ± 0 .4 9 1 .17 No treatment area 2 2 .1 ± 2 .7 3 3 .9 5 ± 0 .7 3 0 .80

[0033] From Table 3, it can be seen that the "Leybacillus" thermospheres of the present invention (Ureibacillus ther

mosDhaericus), address: 305 305-8566 Tsukuba, Ibaraki Pref. 1 1 1 Central No. 6, Name: National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary, April 30, 2004, accession number, FERM It has been found that a soil blending material containing a large amount of a novel thermophilic bacterium, which was accepted as P-20039, has an effect of improving the absorbability of fertilizer. It was also found that the quality was improved by using compost.

As the compost used in the present example, the same raw material was used for the cow dung compost of the present invention and the conventional cow dung compost. Therefore, each compost lg is suspended in water and stirred, then the volume is increased to 1 liter, and the supernatant obtained by centrifugation at 10,000 rpm for 5 minutes is filtered to obtain potassium ions and orthophosphate ions. Was measured. Table 4 shows the results. As is evident from Table 4, even with compost using the same raw material, the compost of the present invention had more than twice the amount of PO ³ ions as compared with the conventional compost. It can also be understood that phosphoric acid is easily absorbed if it is ionized, a force that is said to be difficult to absorb as fertilizer. Potassium ion was also 1.38 times.

[Table 4]

Example 3

Cucumbers (variety, fresh green) were sown in the nursery in early May. The plate was pressed vertically and the surface of the nursery was pressed at 9 cm intervals to form shallow vertical striped grooves on the surface. The seeds were carefully placed side by side at 1.5 cm intervals in this groove, and lightly soiled. Sprouting when watered enough Futaba also lined up sideways. The seedlings were grown with appropriate thinning, leaving good seedlings. At the end of May, four to five seedlings had grown.

[0037] Separately, the field in the house was cultivated to a depth of 20 to 25 cm from late April, and the compost produced in Example 1 (2), 1500 kg ZlOa, and the slaked lime lOkgZlOa were mixed with the soil and mixed well. Chemical fertilizers containing 8% each of nitrogen, phosphoric acid and potash were mixed into the fields as primary fertilizer immediately before planting. In the field, ridges with an upper side of about 60 cm were provided in parallel, and the distance from the center of the ridge to the center was set to about 180 cm. Seedlings that grew approximately 60 cm apart from each other on both sides of the ridge at approximately 50 cm intervals were planted in two rows.

[0038] Next, a standing column is set up beside each seedling, and the two columns standing on the same ridge at a position 2 m above the column at a position perpendicular to the direction of the ridge are crossed and connected. Combined. The intersection is arranged at the center of the ridge in parallel with the ridge. In addition, a long rod was tied on this crossing to support it from falling.

[0039] The seedlings planted on June 1 grew steadily, and when the plant height reached the intersection of the columns, the shoots were picked, and the side branches were picked leaving three leaves. In addition, I picked up the leaves, leaving two leaves. After planting, fertilizer was added approximately every 15 days. The seedlings grew well and on June 15 the first crop of cucumbers about 20 cm long was obtained. Around June 20, the aphid was discovered, so the runnate solution was diluted 1000-fold to lOOOOppm, and the neighborhood where the insects occurred was intensively sprayed, mainly on the back of the leaf, and then on the leaf surface. . The harvest continued until August 29, with no pests, due to sufficient application of pesticides.

[0040] Regarding the residual pesticides in the harvested cucumbers, a box of 50 bottles was brought to the Tokyo Metropolitan Public Health Inspection Service, where they were asked to test for residual pesticides. No residual pesticides were detected for carbamate pesticides. In this case, the detection limit concentrations were all 0.1 Olppm.

Industrial applicability

[0041] A member of the present invention, which belongs to Leybacillus thermosphaericus (Ureibacillus thermosphaericus). Address: τ 305-8566 Tsukuba, Higashi, Ibaraki Prefecture 1 1 1 Central No. 6, Name: National Institute of Advanced Industrial Science and Technology, Patent Organism A new thermophilic bacterium, deposited at the Depositary Center on April 30, 2004, under the accession number, FERM P-20039, is cultured in a medium rich in proteins and polypeptides and is directly or soil-mixed By applying with

Claims

The scope of the claims

[1] Address: T 305-8566 Tsukuba-Higashi, Ibaraki Pref. 1 1 1 Central No. 6, Name: National Institute of Advanced Industrial Science and Technology, Patent Organism Depositary, April 30, 2004, accession number, FER MP— New thermophile belonging to ゥ Raybacillus thermosphaericus (Ureibacillus thermosphaericus) received as No. 20033 ¾L

[2] A novel thermophilic bacterium, which has a partial nucleotide sequence obtained by 16S rDNA nucleotide sequence analysis of the novel thermophilic bacterium according to claim 1, which has a sequence shown in the Sequence Listing.

[3] A compost comprising the novel thermophilic bacterium according to claim 1 or 2 in an amount of 1 × 10 ⁶ cfo / g or more.

[4] A compost comprising the novel thermophilic bacterium according to claim 1 or 2 in an amount of 1 × 10 ⁷ cfo / g or more.

[5] in useful material in agricultural soil, soil incorporated material characterized by containing a novel thermophile 1 X 10 ⁵ clu / g or more according to claim 1 or 2.

[6] A soil compounding material characterized in that the useful thermophilic bacterium according to claim 1 or 2 is contained in a material useful for agricultural soil in an amount of 1 X 10 ⁶ cfo / g or more.

[7] A compost or soil blending material containing at least 1 × 10 ⁵ cfo / g containing the novel thermophilic bacterium according to claim 1 or 2 is applied to the soil where the crops are grown. To grow agricultural products.

[8] A method of applying a compost or a soil blending material containing at least 1 × 10 ⁶ cfo / g containing the novel thermophilic bacterium according to claim 1 or 2 to soil for cultivating agricultural crops. To grow agricultural products.