CN110484464A - It is a kind of for preventing and treating the microorganism formulation and organic fertilizer of the withered line disease of rice - Google Patents
It is a kind of for preventing and treating the microorganism formulation and organic fertilizer of the withered line disease of rice Download PDFInfo
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Abstract
The present invention provides a kind of for preventing and treating the microorganism formulation and organic fertilizer of the withered line disease of rice, it is related to technical field of fertilizers, the microorganism formulation contains bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould, microorganism formulation of the present invention line disease withered for rice has good control efficiency, using the experimental plot either cave disease incidence or strain disease incidence of microorganism formulation of the present invention below 10%, and it can be seen that by comparative test, bacillus thuringiensis in microorganism formulation of the present invention, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould has good synergistic action effect each other.
Description
Technical field
The present invention relates to technical field of fertilizers, and in particular to a kind of microorganism formulation for preventing and treating the withered line disease of rice and has
Machine fertilizer.
Background technique
Rice is the most important cereal crops of China, but the load class Rhizoctonia solani Kuhn (Rhizoctonia of soil-borne infection
Solani K ü hn) rice sheath blight disease that causes is one of harm rice fungus diseases evil the most serious in global range, especially exists
The high yield and stable yields of rice are seriously hindered in intensive cropping system.In recent years as the reinforcing of Rice Production system, plantation are close
The raising of degree and the utilization of high-yield rice kind, banded sclerotial blight are on the rise in the harm of China.When natural conditions are conducive to cause of disease
Bacterium grow when, caused by yield damage may be up to 50%.In addition, the rice sheath blight disease epidemic-stricken area in China is up to 1500 to 2000
Ten thousand hm2, about 6,000,000 t of the production loss having resulted in.The disease because host is extensive, sclerotium long-term surviving in two season crop soil
In, and the characteristic strong with hereditary variability, it makes it difficult to control.
Now, the major measure for preventing and treating rice sheath blight disease has agricultural, chemistry and biological control.Wherein, salvage sclerotium, rationally
Cultivation management and breeding resistant variety are the important means of cultural control, but its effect is limited, and resistant gene is single, transgenosis
Safety and it is difficult to improve the outstanding problems urgent need to resolve such as the lasting validity of resistant variety.And chemical prevention has economy due to it
It efficiently, is always the indispensable part of Synthetical prevention rice sheath blight disease the advantages that wide sterilization spectrum, prevention and treatment simplicity.But excessively
Cause numerous following problems using chemical pesticide, such as: environmental pollution, human health is worsening, banded sclerotial blight is resistance to
Pharmacological property enhancing, or even other beneficial organisms are also compromised when murdering sheath blight fungus, rice field ecology balance is destroyed, is led indirectly
Cause the rice underproduction.Biological control is acknowledged as preventing and treating during modern agricultural production because of good environmental compatible and sustainability
The important channel of rice sheath blight disease, but currently used for prevention and treatment rice sheath blight disease microorganism formulation effect be not it is very good, also need
Want further Improvement.
Summary of the invention
It is an object of the present invention to the microorganism formulation that the present invention provides a kind of for preventing and treating the withered line disease of rice and organic
Fertilizer.
In order to achieve the above object, the present invention is achieved by the following technical programs:
It is a kind of for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the microorganism formulation contains Su Yunjin bud
Spore bacillus (Bacillius thuringiensis, Bt), serratia marcescens (Serratia marcesces), ball spore
Muscardine (Beauveria bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria
(lactic acid bacteria, LAB), black-koji mould (Aspergillus niger).
Further, the microorganism formulation is liquid microbe preparation or powder microorganism formulation.
Further, bacillus thuringiensis 3-6 × 10 in the liquid microbe preparation12Cfu/mL, Serratia
Bacterium 1.2-1.5 × 1012Cfu/mL, beauveria bassiana 2.2-3 × 1012Cfu/mL, Trichoderma asperellum 3-6 × 1011Cfu/mL, cream
Sour bacterium 5-8 × 1010Cfu/mL, black-koji mould 0.1-0.4 × 108cfu/mL。
Further, bacillus thuringiensis 2.3-5 × 10 in the powder microorganism formulation12Cfu/mL, cement sand thunder
Salmonella 1.03-1.47 × 1012Cfu/mL, beauveria bassiana 3.5-4 × 1012Cfu/mL, Trichoderma asperellum 1-2 × 1011cfu/mL
, lactic acid bacteria 0.22-0.48 × 1011Cfu/mL, black-koji mould 2-2.6 × 108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
(1) bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into first by a certain amount of
It is mixed in culture medium, ferment 2.5-5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25-28 DEG C of temperature,
Revolving speed 100-200rpm, shimmy amplitude 10-15mm, air intake 300-500L/h；
(2) lactic acid bacteria, black-koji mould are mixed by a certain amount of be inoculated into the second culture medium, is detested in constant temperature incubation oscillation shaking table
Aerobe fermentation 2.5-5d obtains bacterium solution B, fermentation parameter are as follows: and 25-28 DEG C of temperature, revolving speed 50-80rpm, shimmy amplitude 5-12mm；
(3) it is diluted with water or is concentrated after mixing bacterium solution A with bacterium solution B, dry the microorganism formulation can be obtained.
Further, the first culture medium includes the constituent of following weight fraction:
4-8 parts of beef extract, 20-30 parts of potato, 10-15 parts of peptone, 2-3 parts of sucrose, 0.2-0.4 parts of L-cysteine, chlorine
Change sodium 3-5 parts, 0.1-0.4 parts of potassium dihydrogen phosphate, 1000-1200 parts of deionized water.
Further, the second culture medium includes the constituent of following weight fraction:
5-10 parts of beef extract, 24-28 parts of peptone, 1-3 parts of bean cake powder, 0.1-0.5 parts of potassium dihydrogen phosphate, bagasse 0.5-0.7
Part, 1-1.4 parts of glucose, 1.1-1.4 parts of tween, 0.03-0.07 parts of dibasic ammonium citrate, 0.3-0.5 parts of magnesium sulfate, manganese sulfate
0.6-1.5 parts, 2.5-4 parts of sodium thioglycolate, 1000-1200 parts of deionized water.
Application of the mentioned microorganism preparation in the withered line disease of prevention and treatment rice.
The present invention also provides a kind of organic fertilizer including mentioned microorganism preparation, the ingredient of organic fertilizer includes matrix fertilizer
Material, microorganism formulation and EM bacteria agent, three's weight ratio are 800-1000:1:0.1-0.3；
Matrix fertilizer includes the constituent of following weight fraction:
10-16 parts of barnyard manure, 8-12 parts of castor cake, 1-4 parts of silkworm excrement, 5-7 parts of vinasse, 3-5 parts of bagasse, 1-2 parts of potassium breeze, phosphorus ore
0.5-1 parts of powder, 1-1.5 parts of calcium chloride, 6-12 parts of edible fungi residues, 5-10 parts of manioc waste.
The invention has the following advantages:
Bacillus thuringiensis can produce two major classes toxin, i.e. endotoxin (parasporal crystal) and outer poison in microorganism formulation of the present invention
Element, after pests, under the effect of enteron aisle alkaline digest liquid, thallus discharges toxin, and pest is poisoned and stops feeding, last pest
Because hungry and blood and neurotoxic are dead, and the system of defense of rice can be effectively activated, raising resists the withered line disease of rice
Property；
Serratia marcescens disease can compete identical nutriment in rice leaf and root system with Rhizoctonia solani Kuhn, make its due to
Lack nutriment and dead；
Beauveria bassiana is broad spectrum insecticide fungi, its own can exactly efficiently control population number, while not injure other
Natural enemy insect and beneficial organism；
Trichoderma asperellum can not only secrete organic acid, and that the minerals that plant cannot directly absorb are degraded into phosphorous, potassium etc. is organic
Fertilizer, and the growth that the auxins such as gibberellin, heteroauxin directly facilitate plant can be secreted, it furthermore can also improve plant
Microbiologic population in object root soil promotes the growth of plant indirectly；
Lactic acid bacteria, black-koji mould can be played as collaboration strain further to be promoted to improve rice tillering number, spike number, dry granular quality
With Soil Microorganism to the Utilization ability of nutriment；
Microorganism formulation of the present invention line disease withered for rice has good control efficiency, uses the test of microorganism formulation of the present invention
Field either cave disease incidence or strain disease incidence are 10% hereinafter, and can be seen that microorganism of the present invention by comparative test
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould are mutual in preparation
Between have good synergistic action effect.
Detailed description of the invention
Fig. 1 is the flow chart of microorganism formulation preparation method of the present invention.
Specific embodiment
Embodiment 1:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is liquid microbe preparation；
Wherein, bacillus thuringiensis 3.6 × 1012Cfu/mL, serratia marcescens 1.4 × 1012Cfu/mL, beauveria bassiana
2.5×1012Cfu/mL, Trichoderma asperellum 5 × 1011Cfu/mL, lactic acid bacteria 6.6 × 1010Cfu/mL, black-koji mould 0.13 ×
108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 2.5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 26 DEG C of temperature, revolving speed
150rpm, shimmy amplitude 12mm, air intake 350L/h；First culture medium includes the constituent of following weight fraction: ox
5 parts of meat extract, 22 parts of potato, 14 parts of peptone, 2.2 parts of sucrose, 0.23 part of L-cysteine, 4 parts of sodium chloride, potassium dihydrogen phosphate
0.15 part, 1200 parts of deionized water are mixed lactic acid bacteria, black-koji mould by a certain amount of be inoculated into the second culture medium, Yu Hengwen
Anaerobic fermentation 2.5d in culture oscillation shaking table, obtains bacterium solution B, fermentation parameter are as follows: and 26 DEG C of temperature, revolving speed 80rpm, shimmy amplitude
10mm；Second culture medium includes the constituent of following weight fraction: 8 parts of beef extract, 25 parts of peptone, 1.5 parts of bean cake powder, phosphorus
0.3 part of acid dihydride potassium, 0.55 part of bagasse, 1.2 parts of glucose, 1.2 parts of tween, 0.06 part of dibasic ammonium citrate, magnesium sulfate 0.3
Part, 1 part of manganese sulfate, 3 parts of sodium thioglycolate, 1200 parts of deionized water, are diluted with water or dense after bacterium solution A is mixed with bacterium solution B
The microorganism formulation can be obtained in contracting, drying.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.2；
Matrix fertilizer includes the constituent of following weight fraction:
14 parts of barnyard manure, 8 parts of castor cake, 3 parts of silkworm excrement, 6 parts of vinasse, 4 parts of bagasse, 1 part of potassium breeze, 0.6 part of ground phosphate rock, calcium chloride
1.5 parts, 10 parts of edible fungi residues, 8 parts of manioc waste.
Embodiment 2:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger)；
The microorganism formulation is liquid microbe preparation；
Wherein, bacillus thuringiensis 3 × 1012Cfu/mL, serratia marcescens 1.5 × 1012Cfu/mL, beauveria bassiana 2.3
×1012Cfu/mL, Trichoderma asperellum 4 × 1011Cfu/mL, lactic acid bacteria 5.5 × 1010Cfu/mL, black-koji mould 0.33 ×
108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 28 DEG C of temperature, revolving speed 120rpm,
Shimmy amplitude 15mm, air intake 500L/h；First culture medium includes the constituent of following weight fraction: 8 parts of beef extract,
25 parts of potato, 15 parts of peptone, 3 parts of sucrose, 0.3 part of L-cysteine, 5 parts of sodium chloride, 0.1 part of potassium dihydrogen phosphate, go from
1000 parts of sub- water is mixed lactic acid bacteria, black-koji mould by a certain amount of be inoculated into the second culture medium, vibrates shaking table in constant temperature incubation
Middle anaerobic fermentation 5d, obtains bacterium solution B, fermentation parameter are as follows: and 28 DEG C of temperature, revolving speed 60rpm, shimmy amplitude 10mm；Second culture medium
Constituent including following weight fraction: 10 parts of beef extract, 25 parts of peptone, 3 parts of bean cake powder, 0.5 part of potassium dihydrogen phosphate, sweet
It is 0.6 part of bagasse, 1.4 parts of glucose, 1.2 parts of tween, 0.03 part of dibasic ammonium citrate, 0.5 part of magnesium sulfate, 0.6 part of manganese sulfate, thio
4 parts of sodium glycollate, 1000 parts of deionized water are diluted with water or are concentrated after mixing bacterium solution A with bacterium solution B, dry institute can be obtained
State microorganism formulation.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.1；
Matrix fertilizer includes the constituent of following weight fraction:
10 parts of barnyard manure, 12 parts of castor cake, 3 parts of silkworm excrement, 6 parts of vinasse, 4 parts of bagasse, 1 part of potassium breeze, 1 part of ground phosphate rock, calcium chloride
1.3 parts, 9 parts of edible fungi residues, 8 parts of manioc waste.
Embodiment 3:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is powder microorganism formulation.
Wherein, bacillus thuringiensis 5 × 1012Cfu/mL, serratia marcescens 1.36 × 1012Cfu/mL, the white deadlock of ball spore
Bacterium 3.5 × 1012Cfu/mL, Trichoderma asperellum 1.1 × 1011Cfu/mL, lactic acid bacteria 0.4 × 1011Cfu/mL, black-koji mould 2.5
×108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25 DEG C of temperature, revolving speed 200rpm,
Shimmy amplitude 12mm, air intake 400L/h；
First culture medium includes the constituent of following weight fraction: 5 parts of beef extract, 20 parts of potato, 15 parts of peptone, sucrose
2 parts, 0.4 part of L-cysteine, 4 parts of sodium chloride, 0.1 part of potassium dihydrogen phosphate, 1200 parts of deionized water, by lactic acid bacteria, aspergillus niger
Bacterium is mixed by a certain amount of be inoculated into the second culture medium, and the anaerobic fermentation 5d in constant temperature incubation oscillation shaking table obtains bacterium solution B, sends out
Ferment parameter are as follows: 28 DEG C of temperature, revolving speed 60rpm, shimmy amplitude 10mm；Second culture medium include following weight fraction composition at
Point: 8 parts of beef extract, 3 parts of bean cake powder, 0.5 part of potassium dihydrogen phosphate, 0.5 part of bagasse, 1.4 parts of glucose, is spat 25 parts of peptone
Warm 1.1 parts, 0.05 part of dibasic ammonium citrate, 0.5 part of magnesium sulfate, 1 part of manganese sulfate, 3 parts of sodium thioglycolate, deionized water 1000
Part, it is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 800:1:0.2；
Matrix fertilizer includes the constituent of following weight fraction:
12 parts of barnyard manure, 12 parts of castor cake, 2 parts of silkworm excrement, 6 parts of vinasse, 3 parts of bagasse, 1 part of potassium breeze, 0.6 part of ground phosphate rock, chlorination
1.2 parts of calcium, 10 parts of edible fungi residues, 8 parts of manioc waste.
Embodiment 4:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is powder microorganism formulation.
Wherein bacillus thuringiensis 2.3 × 1012Cfu/mL, serratia marcescens 1.03 × 1012Cfu/mL, ball spore are white
Stiff bacterium 3.5 × 1012Cfu/mL, Trichoderma asperellum 1 × 1011Cfu/mL, lactic acid bacteria 0.22 × 1011Cfu/mL, black-koji mould 2 ×
108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 2.5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25 DEG C of temperature, revolving speed
100rpm, shimmy amplitude 10mm, air intake 300L/h；
First culture medium includes the constituent of following weight fraction: 4 parts of beef extract, 20 parts of potato, 10 parts of peptone, sucrose
2 parts, 0.2 part of L-cysteine, 3 parts of sodium chloride, 0.1 part of potassium dihydrogen phosphate, 1000 parts of deionized water, by lactic acid bacteria, aspergillus niger
Bacterium is mixed by a certain amount of be inoculated into the second culture medium, and the anaerobic fermentation 2.5d in constant temperature incubation oscillation shaking table obtains bacterium solution B,
Fermentation parameter are as follows: 25 DEG C of temperature, revolving speed 50rpm, shimmy amplitude 5mm；Second culture medium include following weight fraction composition at
Point: 5 parts of beef extract, 24 parts of peptone, 1 part of bean cake powder, 0.1 part of potassium dihydrogen phosphate, 0.5 part of bagasse, 1 part of glucose, tween
1.1 parts, 0.03 part of dibasic ammonium citrate, 0.3 part of magnesium sulfate, 0.6 part of manganese sulfate, 2.5 parts of sodium thioglycolate, deionized water 1000
Part, it is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 800:1:0.1；
Matrix fertilizer includes the constituent of following weight fraction:
10 parts of barnyard manure, 8 parts of castor cake, 1 part of silkworm excrement, 5 parts of vinasse, 3 parts of bagasse, 1 part of potassium breeze, 0.5 part of ground phosphate rock, calcium chloride
1 part, 6 parts of edible fungi residues, 5 parts of manioc waste.
Embodiment 5:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contains bacillus thuringiensis (Bacillius
Thuringiensis, Bt), serratia marcescens (Serratia marcesces), beauveria bassiana (Beauveria
Bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria (lactic acid bacteria,
LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is liquid microbe preparation.
Wherein bacillus thuringiensis 6 × 1012Cfu/mL, serratia marcescens 1.5 × 1012Cfu/mL, beauveria bassiana
3×1012Cfu/mL, Trichoderma asperellum 6 × 1011Cfu/mL, lactic acid bacteria 8 × 1010Cfu/mL, black-koji mould 0.4 × 108cfu/
mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into the first culture by a certain amount of
It is mixed in base, ferment 5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 28 DEG C of temperature, revolving speed 200rpm,
Shimmy amplitude 15mm, air intake 500L/h；
First culture medium includes the constituent of following weight fraction: 8 parts of beef extract, 30 parts of potato, 15 parts of peptone, sucrose
3 parts, 0.4 part of L-cysteine, 5 parts of sodium chloride, 0.4 part of potassium dihydrogen phosphate, 1200 parts of deionized water, by lactic acid bacteria, aspergillus niger
Bacterium is mixed by a certain amount of be inoculated into the second culture medium, and the anaerobic fermentation 5d in constant temperature incubation oscillation shaking table obtains bacterium solution B, sends out
Ferment parameter are as follows: 28 DEG C of temperature, revolving speed 80rpm, shimmy amplitude 12mm；Second culture medium include following weight fraction composition at
Point: 10 parts of beef extract, 3 parts of bean cake powder, 0.5 part of potassium dihydrogen phosphate, 0.7 part of bagasse, 1.4 parts of glucose, is spat 28 parts of peptone
Warm 1.4 parts, 0.07 part of dibasic ammonium citrate, 0.5 part of magnesium sulfate, 1.5 parts of manganese sulfate, 4 parts of sodium thioglycolate, deionized water 1200
Part, it is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.3；
Matrix fertilizer includes the constituent of following weight fraction:
16 parts of barnyard manure, 12 parts of castor cake, 4 parts of silkworm excrement, 7 parts of vinasse, 5 parts of bagasse, 2 parts of potassium breeze, 1 part of ground phosphate rock, calcium chloride
1.5 parts, 12 parts of edible fungi residues, 10 parts of manioc waste.
Comparative example 1:
A kind of microorganism formulation for preventing and treating the withered line disease of rice contain serratia marcescens (Serratia marcesces),
Beauveria bassiana (Beauveria bassiana), Trichoderma asperellum (Trichoderma asperellum), lactic acid bacteria
(lactic acid bacteria, LAB), black-koji mould (Aspergillus niger).
The microorganism formulation is liquid microbe preparation.
Wherein, serratia marcescens 1.4 × 1012Cfu/mL, beauveria bassiana 2.5 × 1012Cfu/mL, Trichoderma asperellum 5
×1011Cfu/mL, lactic acid bacteria 6.6 × 1010Cfu/mL, black-koji mould 0.13 × 108cfu/mL。
It is above-mentioned for prevent and treat the withered line disease of rice microorganism formulation the preparation method is as follows:
Serratia marcescens, beauveria bassiana, Trichoderma asperellum are mixed by a certain amount of be inoculated into the first culture medium, Yu Hengwen
Ferment 2.5d in culture oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: and 26 DEG C of temperature, revolving speed 150rpm, shimmy amplitude 12mm,
Air intake 350L/h；First culture medium includes the constituent of following weight fraction: 5 parts of beef extract, 22 parts of potato, egg
14 parts of white peptone, 2.2 parts of sucrose, 0.23 part of L-cysteine, 4 parts of sodium chloride, 0.15 part of potassium dihydrogen phosphate, deionized water 1200
Part, lactic acid bacteria, black-koji mould are mixed by a certain amount of be inoculated into the second culture medium, the anaerobism hair in constant temperature incubation oscillation shaking table
Ferment 2.5d obtains bacterium solution B, fermentation parameter are as follows: and 26 DEG C of temperature, revolving speed 80rpm, shimmy amplitude 10mm；Second culture medium include with
The constituent of lower weight fraction:
8 parts of beef extract, 25 parts of peptone, 1.5 parts of bean cake powder, 0.3 part of potassium dihydrogen phosphate, 0.55 part of bagasse, glucose 1.2
Part, 1.2 parts of tween, 0.06 part of dibasic ammonium citrate, 0.3 part of magnesium sulfate, 1 part of manganese sulfate, 3 parts of sodium thioglycolate, deionized water
It 1200 parts, is diluted with water or is concentrated after bacterium solution A is mixed with bacterium solution B, dry the microorganism formulation can be obtained.
A kind of organic fertilizer includes matrix fertilizer, microorganism formulation and EM bacteria agent, and three's weight ratio is 1000:1:0.2；
Matrix fertilizer includes the constituent of following weight fraction:
14 parts of barnyard manure, 8 parts of castor cake, 3 parts of silkworm excrement, 6 parts of vinasse, 4 parts of bagasse, 1 part of potassium breeze, 0.6 part of ground phosphate rock, calcium chloride
1.5 parts, 10 parts of edible fungi residues, 8 parts of manioc waste.
Comparative example 1 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain bacillus thuringiensis.
Comparative example 2:
Comparative example 2 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain serratia marcescens.
Comparative example 3:
Comparative example 3 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain beauveria bassiana.
Comparative example 4:
Comparative example 4 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain Trichoderma asperellum.
Comparative example 5:
Comparative example 5 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain lactic acid bacteria.
Comparative example 6:
Comparative example 6 the difference from embodiment 1 is that, in the microorganism formulation of comparative example 1 do not contain black-koji mould.
Planting experiment:
The microorganism formulation for being utilized respectively 1-5 of the embodiment of the present invention and comparative example 1-6 handles rice seedling；
Wherein embodiment 1,2,5, comparative example 1-6 are liquid microbe preparation, are diluted to after 1000 times to rice seedling
Carry out root dipping processing；
Embodiment 3,4 is powder microorganism formulation, again by powder microorganism formulation after rice seedling root steam is moistened by spraying
Sprinkling is adhered to its root；
The rice seedling of blank example is not processed.
It is " southern round-grained rice 9108 " for examination rice varieties.Test is in the town the He Feng village Sun Jiaxiang, Nanjing Lishui area.Investigation records
The cave of different disposal and strain disease incidence, disease index, banded sclerotial blight investigation method are carried out according to GB/T17980.20-2000.
Test result is as shown in table 1 below:
Table 1:
By upper table 1 it is found that microorganism formulation of the present invention line disease withered for rice has good control efficiency, the micro- life of the present invention is used
The experimental plot of object preparation either cave disease incidence or strain disease incidence by comparative test 10% hereinafter, and can be seen that
Bacillus thuringiensis in microorganism formulation of the present invention, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria,
Black-koji mould has good synergistic action effect each other.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although with reference to the foregoing embodiments
Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation
Technical solution documented by example is modified or equivalent replacement of some of the technical features；And these modification or
Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (10)
1. a kind of for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the microorganism formulation contains Su Yunjin
Bacillus, serratia marcescens, beauveria bassiana, Trichoderma asperellum, lactic acid bacteria, black-koji mould.
2. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the microorganism system
Agent is liquid microbe preparation or powder microorganism formulation.
3. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the micro- life of liquid
Bacillus thuringiensis 3-6 × 10 in object preparation12Cfu/mL, serratia marcescens 1.2-1.5 × 1012Cfu/mL, the white deadlock of ball spore
Bacterium 2.2-3 × 1012Cfu/mL, Trichoderma asperellum 3-6 × 1011Cfu/mL, lactic acid bacteria 5-8 × 1010Cfu/mL, black-koji mould
0.1-0.4×108cfu/mL。
4. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the micro- life of powder
Bacillus thuringiensis 2.3-5 × 10 in object preparation12Cfu/mL, serratia marcescens 1.03-1.47 × 1012Cfu/mL, ball spore
Muscardine 3.5-4 × 1012Cfu/mL, Trichoderma asperellum 1-2 × 1011Cfu/mL, lactic acid bacteria 0.22-0.48 × 1011cfu/mL、
Black-koji mould 2-2.6 × 108cfu/mL。
5. as described in claim 1 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that preparation method is such as
Under:
(1) bacillus thuringiensis, serratia marcescens, beauveria bassiana, Trichoderma asperellum are inoculated into first by a certain amount of
It is mixed in culture medium, ferment 2.5-5d in constant temperature incubation oscillation shaking table, obtains bacterium solution A, fermentation parameter are as follows: 25-28 DEG C of temperature,
Revolving speed 100-200rpm, shimmy amplitude 10-15mm, air intake 300-500L/h；
(2) lactic acid bacteria, black-koji mould are mixed by a certain amount of be inoculated into the second culture medium, is detested in constant temperature incubation oscillation shaking table
Aerobe fermentation 2.5-5d obtains bacterium solution B, fermentation parameter are as follows: and 25-28 DEG C of temperature, revolving speed 50-80rpm, shimmy amplitude 5-12mm；
(3) it is diluted with water or is concentrated after mixing bacterium solution A with bacterium solution B, dry the microorganism formulation can be obtained.
6. as claimed in claim 5 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the first culture medium packet
Include the constituent of following weight fraction:
4-8 parts of beef extract, 20-30 parts of potato, 10-15 parts of peptone, 2-3 parts of sucrose, 0.2-0.4 parts of L-cysteine, chlorine
Change sodium 3-5 parts, 0.1-0.4 parts of potassium dihydrogen phosphate, 1000-1200 parts of deionized water.
7. as claimed in claim 5 for preventing and treating the microorganism formulation of the withered line disease of rice, which is characterized in that the second culture medium packet
Include the constituent of following weight fraction:
5-10 parts of beef extract, 24-28 parts of peptone, 1-3 parts of bean cake powder, 0.1-0.5 parts of potassium dihydrogen phosphate, bagasse 0.5-0.7
Part, 1-1.4 parts of glucose, 1.1-1.4 parts of tween, 0.03-0.07 parts of dibasic ammonium citrate, 0.3-0.5 parts of magnesium sulfate, manganese sulfate
0.6-1.5 parts, 2.5-4 parts of sodium thioglycolate, 1000-1200 parts of deionized water.
8. such as application of the microorganism formulation of any of claims 1-7 in the withered line disease of prevention and treatment rice.
9. a kind of organic fertilizer, which is characterized in that including microorganism formulation such as of any of claims 1-7.
10. organic fertilizer as claimed in claim 9, which is characterized in that including matrix fertilizer, microorganism formulation and EM bacteria agent, three
Person's weight ratio is 800-1000:1:0.1-0.3；
Described matrix fertilizer includes the constituent of following weight fraction:
10-16 parts of barnyard manure, 8-12 parts of castor cake, 1-4 parts of silkworm excrement, 5-7 parts of vinasse, 3-5 parts of bagasse, 1-2 parts of potassium breeze, phosphorus ore
0.5-1 parts of powder, 1-1.5 parts of calcium chloride, 6-12 parts of edible fungi residues, 5-10 parts of manioc waste.
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