KR101043982B1 - Soil microorganism comprising Lactobacillus fermentum JS and Fertilizer containing thereof - Google Patents

Abstract

The present invention relates to a soil microorganism preparation containing a Lactobacillus perfumant JS strain and a fertilizer containing the same, and more particularly, to a soil microorganism preparation containing a Lactobacillus perfumant JS strain and a fertilizer containing the soil microorganism preparation And a method for producing a fertilizer comprising the soil microbial agent, which comprises the step of adding the soil microbial agent.

The fertilizer containing the soil microorganism preparation of the present invention can simultaneously improve the productivity and quality of crops without using chemical pesticides or chemical fertilizers, and can contribute to environmental conservation by acting as an environmentally friendly fertilizer.

Lactobacillus fermentum JS, soil microorganism preparation, mushroom waste medium,

Description

A soil microorganism preparation containing Lactobacillus fermentum JS strain and a fertilizer containing the same, wherein the soil microorganism comprises Lactobacillus fermentum JS and Fertilizer containing thereof,

The present invention relates to a soil microorganism preparation containing a Lactobacillus perfumant JS strain and a fertilizer containing the same, and more particularly, to a soil microorganism preparation containing a Lactobacillus perfumant JS strain and a fertilizer containing the soil microorganism preparation And a method for producing a fertilizer comprising the soil microbial agent, which comprises the step of adding the soil microbial agent.

The deterioration and deterioration of the soil is a state in which the soil becomes extremely corrupt. In the narrow sense, the rotting soil has many harmful rot ones settled in the soil. In such a soil, organic matter changes into reducing phase gas (hydrocarbons, hydrogen sulfide, ammonia, etc.) and when it is released into the air, it not only damages the roots of crops, but also inhibits the growth due to the production of enzyme inhibitors by secondary metabolism of these substances Of the total. In addition, since the inorganic nutrients are insolubilized in the reduced state, the soil is hardened and there is a tendency that a trace element is liable to be lost.

In particular, the excessive use of chemical fertilizers, especially nitrogen, promotes the decomposition of organic matter in the soil, effectively making the microorganisms in the soil unbalanced. Therefore, microorganisms decomposing decomposition type are always predominantly predominant in the soil, which is a background for increasing the concentration of pathogenic organisms.

Since the use of pesticides and herbicides kill all irrespective of the harmfulness and effectiveness of the microorganisms at the time they are dispersed, the organic matter of the soil remains as a phosphor during degradation, and then the rot fungus, which has an absolute majority in the natural world, becomes particularly dominant. Under the circumstances, the roots of the crops are firmly rooted, they can not grow, and they have to continue to distribute pesticides and fertilizer should be fertilized frequently.

However, even in the case of corruption due to aging or deterioration, when organic matter is fertilized and fermentative microorganisms such as lactic acid bacteria and yeast are maintained predominantly in the long term, the soil is changed into a fermentation type soil. When the microorganisms in the soil are fermented and synthesized, the energy in the soil becomes non-existent as entropy (regardless of the inorganic or organic) and the soil becomes extremely clean. In other words, various obstacles to the activity of the root are eliminated, and the nutrients in the soil are easily eluted, so that even for strong acid and strong alkaline soils, the crops show normal growth. The anaerobic fermentation bacteria are mainly fermented by lactic acid fermentation, and since the inhibition of the bacteria and the solubilization of the organic substances by the lactic acid bacteria proceed at the same time, the reaction proceeds considerably at room temperature. Therefore, the proportion of predominantly spoilage bacteria is very low and the solubilized organic matter is stabilized, and the generation rate of corrosion is also increased and the bacteriocidal power is also strengthened. Such a function is based on a microorganism preparation using a soil microorganism, and a soil microorganism preparation refers to a biological material that helps the cultivation of a plant by utilizing the function of a soil microorganism.

Recently, agricultural production has been transformed into environmentally friendly agriculture using organic farming and microorganisms. In order to satisfy consumers' needs, researches on high quality, functionality and safety (pollution-free agricultural products) have been actively developed , 1998; Kobayashi et al., 1974; Matsuguchi, 1986). The use of microorganisms is focused on increasing the production of food through plant disease control and increasing the physiological activity of the crop itself by taking advantage of the characteristics of bacteria, actinomycetes, mold bacteria, yeast and algae classified as microorganisms. Dong et al. (1999) reported that many of the substances that induce the plant resistance mechanism of plants themselves exhibit plant growth prompting (PGP) at the same time. Shrestha et al. (2003) have developed technologies to isolate and identify bacteria that produce substances that can induce plant growth promoting effects in Korea, and have applied them to the field and achieved many results.

However, as interest in microbiological applications in agriculture has increased, farmers are being confused by the indiscriminate production and sale of microorganisms. Therefore, development and research on microbial agents and the like should be systematically established so that accurate characteristics and efficacy can be verified.

The object of the present invention is to provide eco-friendly agriculture using microbial agents and to develop and research on microbial agents and the like systematically to verify correct characteristics and efficacy, thereby satisfying the needs of consumers, .

In order to solve the above problems,

The present invention provides a soil microbial formulation comprising a strain of Lactobacillus perfumant JS (KCCM 10499) and provides a fertilizer comprising the soil microbial formulation.

The present invention also provides a method for producing a fertilizer comprising a soil microbial agent, which comprises the step of adding a soil microbial agent comprising a strain of Lactobacillus perfumant JS (KCCM 10499) in a method for producing a fertilizer do.

The fertilizer containing the soil microorganism preparation of the present invention can simultaneously improve the productivity and quality of crops without using chemical pesticides or chemical fertilizers, and can contribute to environmental conservation by acting as an environmentally friendly fertilizer.

The present invention provides soil microbial formulations comprising a Lactobacillus fermentum JS (KCCM 10499) strain.

The present invention also provides a fertilizer comprising the soil microbial agent.

In the present invention, the fertilizer may be a fermented compost including at least one selected from the group consisting of swine, bovine, and manure, and a mushroom waste medium and soybean meal.

In the present invention, the fertilizer may be at least one selected from the group consisting of starch foil, beer brew, main anchor, medium foil, citrus foil, apple foil, grape foil, soybean sprout, tofu foil, And at least one selected from the group consisting of glucose, sugar, fructose, oligosaccharides, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, have.

The present invention relates to a method for producing a fertilizer,

The present invention provides a method for producing a fertilizer comprising a soil microbial agent characterized by comprising a step of adding a soil microbial agent containing Lactobacillus fermentum JS (KCCM 10499).

In the method for producing a fertilizer according to the present invention, the fertilizer may be a fermented compost containing at least one selected from the group consisting of swine, bovine and manure, and mushroom waste medium and soybean meal.

Also, in the method for producing a fertilizer according to the present invention, the fertilizer may be at least one selected from the group consisting of starch foil, beer brew, main anchovy, medium foil, citrus foil, apple foil, grape foil, soybean meal, And at least one selected from the group consisting of glucose, sugar, fructose, oligosaccharide, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, And the like.

Hereinafter, the soil microorganism preparation containing the Lactobacillus fermentum JS strain of the present invention and the fertilizer containing the same will be described in detail.

The present invention provides soil microbial preparations containing Lactobacillus perfumant JS strains and thus contributes to mass production of crops and preservation of the environment in that microorganism preparations which are more environmentally friendly than conventional chemical composition preparations are used.

The soil microorganism preparation may contain 1 × 10 ³ to 1 × 10 ¹¹ CFU / g of Lactobacillus perfumant JS strain, preferably 1 × 10 ⁷ CFU / g.

The present invention also provides a fertilizer of high quality comprising the soil microbial agent, and in particular fermented compost or fermentation broth can be produced as a fertilizer.

The fermented compost may include at least one selected from the group consisting of poultry, fowl, and poultry in addition to the soil microorganism preparation, and a mushroom waste medium and soybean meal.

Preferably, the fermentation compost is selected from the group consisting of 0.1 to 1% by weight of the soil microbial preparation, 3 to 10% by weight of at least one selected from the group consisting of poultry, fowl, and manure, 50 to 60% More preferably, the fermentation compost is selected from the group consisting of 0.4% by weight of the soil microbial agent, 4.6% by weight of at least one selected from the group consisting of swine, fowl, and manure, 55% by weight of mushroom waste medium and 40% .

The fermentation broth may contain at least one selected from the group consisting of starch, beer, main anchovy, medium dense, citrus, apple, grape, soybean, tofu, seed, flax, sawdust, And one or more members selected from the group consisting of glucose, sugar, fructose, oligosaccharides, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, paratinose and xylitol.

Preferably, the ratio of the fermentation broth is 0.1 to 1% by weight of the soil microbial preparation, starch powder, brewing, main anchovy, medium nutrition, citrus pine, apple pomelo, grape pomace, soybean pomace, tofu pomace, At least one selected from the group consisting of glucose, sucrose, fructose, oligosaccharides, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, paratinose, xylitol And more preferably 5 to 15% by weight of the fraction and water of the remainder. More preferably, the fermentation broth may comprise 0.4% by weight of the soil microbial preparation, starch powder, beer, main anchovy, At least 15% by weight of at least one selected from the group consisting of medium starch, citrus peel, apple pomace, grape pomace, soybean meal, tofu pomace, seed pomace, flax pomace, sawdust, Stevioside, erythritol, sorbic May include mannitol, maltitol, inositol, El-ku City tolyl, paratinose, 3% by weight of at least one selected from the group consisting of xylitol, manure 10% by weight and the balance water.

The present invention provides a method for producing a fertilizer comprising a soil microbial agent characterized in that it comprises the step of adding a soil microbial agent comprising a strain of Lactobacillus perfumant JS (KCCM 10499) .

Specifically, Lactobacillus fermentum JS strain is cultured, and the culture solution is recovered by centrifugation or the like, followed by drying using a vacuum lyophilizer, and then the soil is treated with excipients such as glucose, skim milk powder and starch, It is manufactured by microbial agent.

The fermented compost or the fermentation broth can be prepared by using the soil microorganism preparation.

The fermented compost is prepared by adding a soil microorganism preparation containing Lactobacillus fermentum JS strain to a basic constitution of fermented compost including mushroom waste medium and soybean meal and at least one selected from the group consisting of swine, ~ 3 days a day, and then fermenting at room temperature for 30 ~ 60 days. The compost after the production can be produced with good quality compost which has no bad smell in smell.

Preferably, the fermentation compost is selected from the group consisting of 0.1 to 1% by weight of the soil microbial preparation, 3 to 10% by weight of at least one selected from the group consisting of poultry, fowl, and manure, 50 to 60% More preferably, the fermentation compost is selected from the group consisting of 0.4% by weight of the soil microbial preparation, 4.6% by weight of at least one selected from the group consisting of poultice, fowl, and manure, 55% by weight of mushroom waste medium and 40% %. ≪ / RTI >

The fermentation broth has at least one selected from the group consisting of starch, beer, main anchovy, medium dense, citrus, apple, grape, soybean, tofu, seed, flax, sawdust, The basic constitution of the fermentation broth containing at least one selected from the group consisting of fructose, oligosaccharides, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, After adding the soil microorganism preparation containing the Bacillus pertomment JS strain, they are agitated for 2 to 3 times a day for 20 to 30 minutes at a time, and incubated at room temperature for 10 to 20 days or in a condition capable of controlling the temperature In a tank, it can be produced by fermentation at 40 to 50 DEG C for 3 to 7 days.

Preferably, the ratio of the fermentation broth is 0.1 to 1% by weight of the soil microbial preparation, starch powder, brewing, main anchovy, medium nutrition, citrus pine, apple pomelo, grape pomace, soybean pomace, tofu pomace, At least one selected from the group consisting of glucose, sucrose, fructose, oligosaccharides, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, paratinose, xylitol And 5% to 15% by weight of the fraction and water of the remainder, and more preferably the fermentation broth may comprise 0.4% by weight of the soil microbial agent, starch powder, 1 to 15% by weight of at least one selected from the group consisting of anchovy, medium dense, citrus, apple, grape, soybean, tofu, noodle, flax, sawdust, mushroom pheasant, glucose, sugar, fructose, oligosaccharide, Aspartame, Stevioside, Ellis Lee It can be prepared, including sorbitol, mannitol, maltitol, inositol, El-ku City tolyl, paratinose, at least one selected from the group consisting of xylitol 3% by weight, based minutes 10% by weight and the balance water.

According to a preferred embodiment of the present invention, a fertilizer containing a soil microorganism preparation using Lactobacillus fermentum JS strain during lettuce cultivation (hereinafter referred to as a "soil microorganism fertilizer") was cultivated in a cultivation or control And showed higher watering effect. Chlorophyll contents were also significantly higher in soil microbial fertilizer treatments. And it is expected that it can be universally used for the cultivation of leafy vegetables consumed in fresh state such as lettuce, and also contributes to consumer stability because no effect of soil microbial fertilizer treatment is observed at all.

The fertilizer containing the soil microorganism preparation of the present invention is fertilized in the crop to promote the growth of the crop, and the effect of improving the acid soil by using it for a long time is expected.

Hereinafter, embodiments and experimental examples of the present invention will be described. It is to be understood, however, that these examples and experiments are for the purpose of illustrating the present invention in detail, and the scope of the present invention is not limited thereto.

Example 1 Preparation of Soil Microorganism Preparation

1. Culture of Lactobacillus fermentum JS strain

1) Composition of medium: 400 g of glucose, 400 g of soybean extract powder, 50 g of K ₂ HPO ₄ , 40 g of sodium acetate, 10 g of MgSO _{4 and} 10 g of Tween-80 were dissolved and dissolved in a mixture of 20 ℓ of water .

2) Seed culture: 600 ml of MRS medium was inoculated with lactobacillus fermentum JS cotyledon colonies, followed by seed culture at 35 ° C for 5 days.

3) Lactic acid bacteria culture: 20 L of water was added to the culture tank, and the ingredients of the culture medium were added at a mixing ratio of 1), sterilized at 121 캜 for 30 minutes, and then cooled to 35 캜. 2). The cells were incubated at 35 ° C for 3 days and centrifuged at 15,000 rpm for cell separation. Cells were lyophilized and stored at -70 ° C.

2. Manufacture of Soil Microbial Agents

The cultured Lactobacillus fermentum JS strain was recovered by centrifugation or the like and then dried using a vacuum freeze dryer to prepare a soil microorganism preparation using excipients such as glucose, skim milk and starch.

Example 2 Preparation of Fermented Compost including Soil Microorganism Preparation

0.1% by weight of the soil microbial preparation prepared in Example 1 above was added to the basic constitution of the compost comprising 4.6% by weight of pork, 55.3% by weight of mushroom waste medium and 40% by weight of soybean meal. They were mixed evenly every 60 hours and fermented at room temperature for 45 days to produce fermented compost.

Example 3 Preparation of Fermented Compost including Soil Microorganism Preparation

0.4% by weight of the soil microbial preparation prepared in Example 1 above was added to the basic constitution of the compost including 4.6% by weight of pork, 55% by weight of mushroom waste medium and 40% by weight of soybean meal. The fermented compost was fermented for 45 days at room temperature by mixing them once every 60 hours.

Example 4 Preparation of Fermented Compost including Soil Microorganism Preparation

1% by weight of the soil microbial preparation prepared in Example 1 above was added to the basic composition of compost containing 4.6% by weight of pork, 54.4% by weight of mushroom waste medium and 40% by weight of soybean meal. The fermented compost was fermented for 45 days at room temperature by mixing them once every 60 hours.

Example 5: Preparation of a fermentation broth containing a soil microbial preparation

The basics of manure containing 15% by weight of starch foil, 5% by weight of beer and main anchovy, the same amount (1% by weight) of glucose, 3% by weight of sugar and fructose, 10% by weight of stems and 71.9% 0.1% by weight of the soil microbial preparation prepared in Example 1 was added to the composition. The fermentation broth was fermented at room temperature for 15 days by stirring them twice a day for 25 minutes at a time.

Example 6: Preparation of a fermentation broth containing a soil microbial preparation

The basics of manure containing 15% by weight of starch foil, 5% by weight of beer and main anchovy, the same amount (1% by weight) of glucose, 3% by weight of sugar and fructose, 10% by weight of stems and 71.6% 0.4 wt% of the soil microbial preparation prepared in Example 1 was added to the composition. The fermentation broth was fermented at room temperature for 15 days by stirring them twice a day for 25 minutes at a time.

Example 7 Preparation of a Fermentation Liquor Ratio Containing a Soil Microorganism Preparation

The basics of manure containing 15% by weight of starch foil, 5% by weight of beer and main anchovy, the same amount (1% by weight) of glucose, 3% by weight of sugar and fructose, 10% 1% by weight of the soil microbial preparation prepared in Example 1 was added to the composition. The fermentation broth was fermented at room temperature for 15 days by stirring them twice a day for 25 minutes at a time.

The following experiment was conducted using the soil microorganism-formulated fertilizer produced by the above-mentioned example. In particular, the present inventors have focused on proving the remarkable effects of the present invention through comparison with a movie ritual and a control.

<Experimental Example 1>

1. Test setting criteria for identification of changes in soil chemical properties by soil microbial fertilizer treatment

To investigate the changes in physicochemical properties of soils after the soil microbial fertilizer prepared in the above Examples, the soil before cultivation and the soil after cultivation were respectively collected and analyzed according to the criteria of analysis items as shown in Table 1 below .

<Table 1> Analytical items on the chemical properties of soils and equipment used

Analysis item Analysis method Used equipment pH 1: 5 distilled water extraction pH meter EC 1: 5 distilled water extraction EC meter Available P ₂ O ₅ Bray No. 1 method UV / Vis spectrometer 0M (total organic content) Walkley-Black method UV / Vis spectrometer K, Ca, Mg Atomic absorption method AAS

2. Analysis of effects of soil microbial fertilizer treatment on crops Test conditions

- Disclosed varieties: 'Chongchima lettuce' (Dongbu Han Agricultural Chemical Seedling Producing Department)

- Cultivation Method: Cultivation of vinyl house land

- Test pavement 1: After 30 days of planting,

- Test pavement 2: It is cultivated for 25 days after planting, and harvested after harvesting (silt loam cultivation)

- Treatment method: Each fertilizer is diluted 250 times before formaldehyde treatment and tillage

- Test group placement method: nodule method 3 repetition (see Fig. 1)

- Treatment: The raw material, control, soil microorganism fertilizer treatment

- Main survey items: physiological characteristics and outline characteristics (leaf width, leaf number, weekday, leaf length, width, chlorophyll content)

N: P: K = 5.1: 4.9: 4.3 "was applied to the soil and the control (artificially mixed with nitrogen, phosphoric acid and potassium) The treatments were such that the crops (green lettuce lettuce) were fertilized and the fertilizers produced by the above examples were sufficiently sprayed thereon and tilled. The root was fertilized 3g per 20g of N: P: K = 5.1: 0: 4.3 for 20 days after the formulation. The test specimens were arranged in 3 replicates by 20 batches per treatment (Fig. 1: Arrangement and schematic diagram of the test pavement, A: movie pod, B: control, C: soil microbial fertilizer).

3. Results of analysis of bacterial counts of soil microbial fertilizers

The contents were determined in accordance with the method of quality inspection of the fertilizer according to the Notification No. 2005-27 of the Rural Development Administration and the sampling standards. The results are shown in Table 2 below.

The results of analysis of the bacterial content of the soil microorganism-formulated fertilizer (the average content of the bacteria collected from the fertilizer produced by the above embodiments)

object Effective microorganism
Identification result Content (cfu / g) Soil microbial fertilizer Lactobacillus fermentum JS 1.3 x 10 ⁷

4. Analysis of chemical characteristics of soils by treatment with soil microbial fertilizer

First, the chemical properties of soil samples were investigated. As a result, it was found that the pH was reasonable when compared with the appropriate range for growing the crops, and that the salt was not accumulated in the EC range. The total organic content (OM) was also at an appropriate level, and both the phosphoric acid and the substitutional cations K, Ca and Mg were in the appropriate range or lower (see Table 3 below).

<Table 3> Soil Chemical Properties before Test Packaging

Analysis item unit Test Package 1 Test Package 2 Appropriate range pH - 6.17 6.22 6.0 - 7.0 EC ds / m 0.77 1.54 2.0 or less OM % 2.96 2.87 2.0 - 3.0 P ₂ O ₅ ppm 421 597 400 - 500 K me / 100g 1.52 0.93 0.70 - 0.80 Ca me / 100g 1.46 3.27 5.0 - 6.0 Mg me / 100g 0.26 1.24 1.5 - 2.0

(Table 3 above is the average of the soil of 3 repetitions)

In addition, soil microbial fertilizer was treated to cultivate the crops, and the chemical characteristics of the soil were analyzed (see Table 4 below). However, in this case, the analysis results of the above embodiments are almost similar to each other, and the description will be made based on the result of analysis based on the third embodiment. As a result, no change in the chemical properties of the soil was observed to affect the growth of crops in both test pack 1 and test pack 1.

<Table 4> Chemical properties of soil after cultivation

Analysis item unit Test Package 1 Test Package 2 Appropriate range pH - 6.91 6.88 6.0 - 7.0 EC ds / m 0.79 0.88 2.0 or less OM % 2.79 2.33 2.0 - 3.0 P ₂ O ₅ ppm 323.1 472.4 400 - 500 K me / 100g 0.74 0.81 0.70 - 0.80 Ca me / 100g 2.27 3.23 5.0 - 6.0 Mg me / 100g 1.72 1.97 1.5 - 2.0

&Lt; Experimental Example 2 > Effect of test packaging on plant growth and yield

1. Results from Test Package 1

The effects of Lactobacillus fermentum JS (KCCM 10499) on the growth and yield of the soil microbial fertilizer treated by lettuce cultivation were shown in Table 5 and Table 6 below. Table 5 shows the results of the growth of the crops prepared in Examples 2 to 4, and Table 6 shows the results of the crops prepared by the methods of Examples 5 to 7 above.

<Table 5> Effects of Soil Microorganism Fertilizer (Fermented Compost) on Growth and Yield of Lettuce Cultivation

process Leaf width (cm) Leaves Weekday (g) Leaf length (cm) Weight (cm) Movie Rokgu 12.2 ^{b *} 21.0 ^c 119.2 ^c 21.6 ^c 34.3 ^c Control 13.3 ^ab 23.1 ^b 135.8 ^b 23.1 ^b 38.1 ^b Soil microbial agent fertilizer treatment (Example 2) 14.4 ^a 26.9 ^a 145.6 ^a 26.6 ^a 41.1 ^a Soil microbial agent fertilizer treatment (Example 3) 15.2 ^a 27.0 ^a 148.0 ^a 28.3 ^a 42.6 ^a Soil microorganism fertilizer treatment (Example 4) 14.9 ^a 26.9 ^a 144.7 ^a 28.1 ^a 41.2 ^a

(The different superscripts in the same vertical row represent significance levels at Duncan's multiple range test at p <0.05).

<Table 6> Effect of soil microbial fertilizer (fermentation broth ratio) on growth and yield of lettuce cultivation

process Leaf width (cm) Leaves Weekday (g) Leaf length (cm) Weight (cm) Movie Rokgu 12.2 ^{b *} 21.0 ^c 119.2 ^c 21.6 ^c 34.3 ^c Control 13.3 ^ab 23.1 ^b 135.8 ^b 23.1 ^b 38.1 ^b Soil microbial agent fertilizer treatment (Example 5) 14.2 ^a 26.3 ^a 145.7 ^a 25.9 ^a 41.0 ^a Soil microbial agent fertilizer treatment (Example 6) 14.8 ^a 26.5 ^a 147.9 ^a 28.1 ^a 42.2 ^a Soil microbial agent fertilizer treatment (Example 7) 14.4 ^a 26.5 ^a 143.9 ^a 27.5 ^a 40.6 ^a

(The different superscripts in the same vertical row represent significance levels at Duncan's multiple range test at p <0.05).

From the results shown in Tables 5 and 6, it can be seen that the growth and the yield of the crop treated with the soil microorganism-formulated fertilizer of the present invention are better than that of the control plot.

In addition to the visual appearance and the above appearance, the result of treatment with soil microbial fertilizer during the cultivation of lettuce on the chlorophyll content was also shown in FIG. 2 (the crops obtained using the fertilizer produced in Example 3, Respectively.

(SPAD-502), 22.8 in the control, and 23.1 in the soil microbial fertilizer treatment (Fig. 2).

2. Results from Test Package 2

The effects of Lactobacillus fermentum JS (KCCM 10499) on the growth and yield of the soil microbial fertilizer treated by lettuce cultivation were shown in Tables 7 and 8 below. Table 7 shows the growth results of the crops prepared by the methods of Examples 5 to 7 and Table 8 of Examples 2 to 4 above.

<Table 7> Effects of soil microbial fertilizer (fermented compost) on the growth and yield of lettuce cultivation

process Leaf width (cm) Leaves Weekday (g) Leaf length (cm) Weight (cm) Movie Rokgu 11.8 ^{b *} 20.9 ^c 118.9 ^c 21.4 ^c 34.2 ^c Control 13.2 ^ab 22.9 ^b 135.4 ^b 23.0 ^b 37.6 ^b Soil microbial fertilizer
Treatment (Example 2) 14.2 ^a 26.7 ^a 144.5 ^a 26.4 ^a 40.5 ^a Soil microbial fertilizer
Treatment (Example 3) 15.2 ^a 27.8 ^a 144.9 ^a 27.7 ^a 42.2 ^a Soil microbial fertilizer
Treatment (Example 4) 14.1 ^a 26.3 ^a 144.8 ^a 26.5 ^a 41.1 ^a

(The different superscripts in the same vertical row represent significance levels at Duncan's multiple range test at p <0.05).

<Table 8> Effect of soil microbial fertilizer (fermentation broth ratio) on growth and yield in lettuce cultivation

process Leaf width (cm) Leaves Weekday (g) Leaf length (cm) Weight (cm) Movie Rokgu 11.8 ^{b *} 20.9 ^c 118.9 ^c 21.4 ^c 34.2 ^c Control 13.2 ^ab 22.9 ^b 135.4 ^b 23.0 ^b 37.6 ^b Soil microbial fertilizer
Treatment (Example 5) 14.4 ^a 26.1 ^a 143.7 ^a 26.0 ^a 40.0 ^a Soil microbial fertilizer
Treatment (Example 6) 14.9 ^a 27.3 ^a 144.6 ^a 27.1 ^a 41.8 ^a Soil microbial fertilizer
Treatment (Example 7) 14.6 ^a 26.5 ^a 144.0 ^a 26.3 ^a 40.5 ^a

(The different superscripts in the same vertical row represent significance levels at Duncan's multiple range test at p <0.05).

From the results shown in Tables 7 and 8, it can be seen that the growth and the yield of the crop treated with the soil microbial fertilizer of the present invention are better than that of the control plot.

In addition, the result of treatment with soil microbial fertilizer on the chlorophyll content during the cultivation of lettuce as well as the visual appearance and the above appearance was also shown in FIG. 3 (the crop obtained using the fertilizer produced in Example 3, Respectively.

(SPAD-502), 22.3 in the control, and 22.8 in the soil microbial fertilizer treatment (Fig. 3).

The increased chlorophyll content of the soil microbial fertilizer treated with Lactobacillus fermentum JS (KCCM 10499), which is a vegetable lactic acid bacterium during the cultivation of the crop, promotes the activity of the plant and promotes the growth as shown in Tables 5 to 8 It can be said that it proves that it can improve the productivity increase.

As a result, the soil microbial fertilizer of the present invention was found to have an effect on the productivity due to the promotion of growth when the soil was treated with the cultivation of the crop. Therefore, it is expected that the present invention can contribute to the productivity of eco-friendly agriculture by continuing research on cultivation of eco-friendly agricultural products using the present invention and using it more aggressively in agriculture field.

The present invention provides an environmentally friendly fertilizer using a soil microbial agent to increase the yield of the crop to increase the profit of the farmer and satisfy the demand of the consumers who consume the crops as well as to prevent environmental pollution and ultimately contribute to the development of the national industry You can do it.

Fig. 1 shows the arrangement and schematic diagram of the treatment pest of the test pavement (A: movie trough, B: control, C: soil microbial fertilizer treatment).

Figure 2 shows the effect of soil microbial fertilizer treatment on the chlorophyll content of test pavement 1 (Duncan's multiple outcome at the 5% level).

Figure 3 shows the effect of soil microbial fertilizer treatment on the chlorophyll content of test pavement 2 (Duncan's multiple outcome at the 5% level).

Claims (7)

A soil microbial formulation comprising Lactobacillus fermentum JS (KCCM 10499). A fertilizer comprising the soil microbial agent of claim 1. [Claim 3] The fertilizer according to claim 2, wherein the fertilizer is a fermented compost comprising at least one selected from the group consisting of swine, bovine, and manure, and a mushroom waste medium and soybean meal. The method of claim 2, wherein the fertilizer is selected from the group consisting of starch foil, beer brew, main anchovy, medium foil, citrus foil, apple foil, grape foil, soybean meal, tofu foil, At least one selected from the group consisting of glucose, sugar, fructose, oligosaccharides, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, A fertilizer comprising a soil microbial agent characterized by a beam. In the method for producing a fertilizer, A method for producing a fertilizer comprising a soil microbial agent, which comprises the step of adding the soil microbial agent of claim 1. [Claim 6] The method according to claim 5, wherein the fertilizer is a fermented compost comprising at least one selected from the group consisting of swine, fowl, and manure, a mushroom waste medium, and soybean meal. 6. The method according to claim 5, wherein the fertilizer is selected from the group consisting of starch foil, beer brew, main anchovy, medium foil, citrus foil, apple foil, grape foil, soybean sprout, tofu foil, At least one selected from the group consisting of glucose, sugar, fructose, oligosaccharide, aspartame, stevioside, erythritol, sorbitol, mannitol, maltitol, inositol, quercitol, Lt; RTI ID = 0.0 &gt; a &lt; / RTI &gt;

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