KR100713059B1 - New rhizopus oligosporus kccm 10624, and the promoter of plant growth and the resistance to plant pathogene comprising thereof - Google Patents

Abstract

The present invention relates to a new strain Lysopus oligosporus KCCM 10624, which has an excellent effect on the growth of plants, and more particularly to the new strain Lysopus oligosporus KCCM 10624, its pure culture, or pure culture thereof. It relates to a method for promoting plant growth using a microbial agent containing as a component and a plant disease resistance enhancer.

Plant growth promoter, plant disease resistance enhancer                                    

Description

Novel Rhizopus oligosporus KCCM 10624, and the promoter of plant growth and the resistance to plant pathogene comprising

1 is an optical micrograph of the new strain Lysopus oligosporus KCCM 10624. (A) is a photograph observed at 240 times, and (B) is a photograph observed at 1200 times.

Figure 2 shows the effect of suppressing the fungal disease of the new strain Lysopus oligosporus KCCM 10624.

Some types and characteristics of microorganisms useful for agriculture are as follows. First of all, lactic acid bacteria grow very rapidly at 20-40 ℃ with or without air and produce lactic acid during the culture process, lower acidity (pH 4) and produce hydrogen peroxide (H ₂ O ₂ ) to inhibit the growth of pathogenic microorganisms. It is known to prevent soil pests. In addition, by producing various physiologically active substances (vitamins, amino acids, nucleic acids, etc.), antibacterial substances, and anticancer substances, crops increase the self-defense ability of plants. It turns out. In addition, yeast is a major microorganism of heat generation during fermentation of agricultural by-products such as rice bran and bran, and has excellent ability to decompose organic matter and shows fermentability and viability under various conditions. Therefore, it is known to exert an odor removal effect when spraying on odor generating sources (barn floor, etc.). Actinomycetes are beneficial microorganisms that grow in soil and produce antibiotics that kill pathogens or stop their growth as natural enemies of pathogenic fungi. Activated Soil of High Quality Soil containing lots of organic material has a high density of actinomycetes. Soil containing high organic matter during fermentation has a high density of actinomycetes. When the compost fermentation temperature is 70 ~ 80 ℃, the ability to control the pests of soil in the compost is due to the large amount of antibiotics produced by actinomycetes. In addition, the unique smell of soil is the smell of actinomycetes. The reason for sterilizing the mushroom medium after fermentation is also to cultivate actinomycetes, and it is also widely known that the well-preserved actinomycetes have almost no contamination of various germs during the sticking process after inoculation. In addition, a fungus called Mycorrhiza grows in close contact with crop roots, absorbs poorly soluble components in the soil (especially phosphoric acid) and provides them to the crop, and also acts as a physical barrier on the root surface to prevent root diseases. have. In addition, there is a photosynthetic bacterium that performs photosynthesis using sunlight as if it is a plant. The bacterium has a crimson color, has a high protein content in the cells, and is known to produce various vitamins, minerals, bioactive substances and antibacterial and antiviral substances. It grows while ingesting various odor generating substances such as ammonia, hydrogen sulfide, and organic acids, and may exhibit an odor removal effect. Photosynthetic bacteria also play a role in gas removal to prevent gas damage due to the use of immature oil in the house, and also has the ability to purify contaminated dirty water, which can be used as a microorganism for treatment of fish farms. Widely used as a microbial insecticide, B / T bacteria produce toxins in the cells, and when the larvae of pests (blue worms, beetroot moths) eat this toxin, the intestines are destroyed and die. Recently, B / T bacteria, which are harmful toyae nematodes, have been developed.

In recent years, many researchers have made efforts to change the microbial phase in the soil in order to benefit plant growth and human health. Bacterization refers to the inoculation of microbial cultures into crop lengths or growing areas, which sometimes leads to a significant increase in crop yield, but it is still not clear what role the inoculated microorganisms play in the root zone. It's not clear. Some microorganisms cannot survive in the root zone by being rejected by the original existing root zone microorganisms, as long as there is a kind that maintains its density by stably surviving and growing in the root zone within the crop growth period. At this time, the microorganisms that stably settle and proliferate at the root surface are called 'root zone microorganisms'.

The rooting settlement of microorganisms is a very active process, in which the microorganisms survive on the seed surface or in the soil and grow using carbohydrate and amino acid-rich seed secretions and continue to grow along the roots attached and growing on the root surface. . In addition, the rhizosphere microbes are passively moved to the lower part of the root by watering.

Representative rhizosphere microorganisms include Pseudomonas, azotobacter, and Bacillus. 'Is called. In the past, the beneficial effects of plant-derived root-root microorganisms were only recognized for root crops such as radish, potatoes and sugar cane, but recently, the positive effects have been recognized in various crops such as oats, beans, cotton, peanuts, balsam, rice and vegetables. .

At present, the use of chemical fertilizers or pesticides is inevitable because we have to increase the absolute yield of crops to provide enough food for population growth. They also face the problem of environmental pollution, which threatens the entire ecosystem due to their use. In this dilemma, the direction of protecting our agriculture and preserving the environment is the use of environmentally friendly biological pesticides and fertilizers.

Therefore, there is a need for the development of environmentally friendly biological pesticides and fertilizers.

Growth promotion and pest control effects by microbial inoculation should be recognized on both sides of the coin. That is, many researchers have revealed that the microorganisms having a growth promoting effect have a pest suppression effect, and that the microorganisms with a disease inhibiting function promote plant growth. For example, some microorganisms (Pseudomonas bacteria) that have been shown to promote plant growth have been shown to promote relative growth of crops by reducing the density of harmful bacteria and fungi in the rhizosphere.

That is, most of the plant growth promoting root zone microorganisms known so far have been known to promote crop growth indirectly by controlling harmful root zone microorganisms.

On the other hand, rhizosphere microorganisms sometimes produce a substance (physiologically active substance) that promotes plant growth, thereby directly promoting plant growth. That is, these bioactive substances are known to exert effects such as promoting root hair growth, promoting root and stem growth by promoting nutrient absorption by the root. Eventually, plant growth-promoting root-strain microorganisms, in addition to the growth promoting effect by pest control, produce special physiologically active substances to change crop physiology to double the plant's self-defense ability against invading germs, thereby preventing disease and promoting growth. .

Biological control mechanisms by rhizosphere microorganisms are known to be due to antimicrobial activity, competition, lysis, specific nutrient depletion, cyan production, and the like.

In the case of antimicrobial activity, rhizosphere microorganisms in which antimicrobial activity (antagonism) against pathogens is stabilized in the laboratory do not necessarily exhibit the same antimicrobial activity in crop cultivation sites, but in many cases, there is a deep correlation.

Antimicrobial activity is mainly due to antibiotics and siderophore secreted by rhizosphere microorganisms, but the main effect of pest biocontrol is mainly due to antibiotics. For example, some plant growth-promoting root zone microorganisms secrete a powerful antibiotic called phenazine around the roots of wheat when administered to wheat cultivated soil, thereby suppressing pathogens and increasing yields.

Competition means the effect of inhibiting pathogens by incapacitating pathogens by competition between root-strain microorganisms and pathogens against the root-sensitive nutrients and root-sensitive areas of the roots. Pseudomonas microorganisms (Pseudomonas bacteria) promote plant growth by rapidly ingesting various nutrients and depleting essential nutrients for use by pathogens. By preoccupying the site, pathogens are prevented from invading the roots, thereby preventing the occurrence of disease.

Lycobacterium refers to the mission of pathogenic fungi by the action of fungal destructive enzymes produced by plant-promoting rhizosphere microorganisms. In other words, plant growth-promoting root zone microorganisms produce a fungal cell wall lytic enzyme called chitinase to destroy the cells of hospital fungi (pisium, etc.), thereby preventing and increasing disease effects.

Iron, one of the trace elements, is an essential element of microbial growth, but iron is insoluble in trivalent iron and cannot be directly used for microorganisms. Microorganisms can utilize iron by producing a substance called ciderofo in order to absorb and utilize these insoluble iron and making a chelate compound having a cederopo-iron. Promote plant growth By producing a large amount or excellent function of side-rope microorganisms, iron is rapidly used to deplete the iron components necessary for the growth of harmful microorganisms (including pathogens) in the root zone. You will not be able to break into roots.

Many rhizospheres produce cyanide compounds, which contribute to pathogen control. In other words, the cyan compound can inhibit the growth by causing fatal damage to the pathogens of the root zone.

Foreign countries have been focusing on PGPR for a long time, and biocontrol agents using plant-promoting rhizosphere microorganisms have already been registered and commercialized, and the trade name "Quantum 4000" is one example. "Quantum 4000" is commercially available as a growth promoter for peanuts, and has been shown not only to promote growth but also to control diseases in peanuts and cotton. This product is composed mainly of microorganisms called Bacillus subtilis, and has no pesticide pollution and has excellent effects and is recognized as a representative success case of pest control using microorganisms.

It is an object of the present invention to provide a new strain of Lysopus oliporus which is effective in promoting plant growth and increasing plant disease resistance.

The present invention is to provide a new strain Lysopus oligos tmporous KCCM 10624 which promotes plant growth and is effective in preventing plant diseases.

The present invention is to provide a microbial agent having a plant growth promoting effect containing a pure culture of the new strain Lysopus oligoporous KCCM 10624 as an active ingredient and a method for promoting plant growth using the same.

The present invention is to provide a microbial agent for improving plant disease resistance containing a pure culture of the new strain Lysopus oligoporous KCCM 10624 as an active ingredient and a method for enhancing plant disease resistance using the same.

The present invention provides a new strain Rhizopus oligosporus KCCM 10624.

The present invention provides a microbial agent having a plant growth promoting effect containing the pure culture of the new strain Lysopus oligophosphorus KCCM 10624 as an active ingredient.

The present invention provides a method for promoting plant growth using the microbial agent.

The present invention provides a method for enhancing resistance to plant diseases to plants using the microbial agent.

Plants that may be used according to the invention may be monocotyledonous or dicotyledonous plants, preferably radishes, cabbage, cabbage, pepper, eggplant, onions, leeks, garlic, lettuce, carrots, celery, cucumbers, green tea, tomatoes, It is selected from the group consisting of strawberry, ginger, watermelon, melon, spinach and pumpkin.

Hereinafter, the present invention will be described in more detail with reference to Examples.

However, the following examples are merely to illustrate the present invention, but the content of the present invention is not limited to the following examples.

Example 1 Isolation Identification and Characteristics of Strains

The new strain Lysopus oligosporus KCCM 10624 was isolated from the analysis of compost with good growth promoting effect. During the opening of the compost to promote the growth of the plant was confirmed excellent compost growth capacity, the microorganisms were separated from the inoculation again to check the efficacy of the compost. Among them, microorganisms having excellent efficacy were isolated, and as a result of the isolation and identification, it was identified as Lycopus oligosporus, and it was deposited with Korea Microorganism Conservation Center on November 25, 2004 and was given accession number KCCM 10624.

Characteristics of Strains of Lysopus Oligosporus

1. Cultural features

It is incubated in PDA (300 g of potato extract, 20 g of glucose, 15 g of agar, 1 l of distilled water) and incubated at 25-30 ° C.

2. Morphological features

When the plate is cultured, the color is white, and the broth grows into grains having a diameter of about 0.5 cm.

3. Features of Mycelia and Spores

The spores were very small and had a spore-like appearance. They had junctional spores and were identified as Lysopus oligospores based on the size of the spores. Figure 1 (A) is a photograph at 240 times the optical microscope of the new strain Lysops oligospores KCCM 10624, (B) is a photograph of the Lysops oligospores KCCM 10624 observed at a magnification of 1200 times.

Example 2 Confirmation of Plant Growth Promoting Effect

end. Materials and methods

(1) Cultivation test site

 Yeongdong Farm, 95 Beoljeong-ri, Sinjeon-myeon, Gangjin-gun, Jeollanam-do South Korea

(2) disclosure crops

Lettuce (red lettuce)

(3) cultivation period

2003.7. 1 ~ 2003. 8. 10

(Sow: July 1, 2003, Formally: July 10, 2003)

(4) test design

-Growth and quality comparison of the treated, Rhizopus oligosporus culture (powder), control and untreated sections

-3 repeated treatments for each oral egg mass method (20m ² growing area per repetition)

-360 weeks of disclosure by cultivating 60 weeks each

(5) Treatment method

(A) Treatment concentration and method

-Microorganism treatment zone: Spread the test sample (powder) 2kg per 1a of test packaging, tilling, and after 2-4 weeks, set the starting material and mix 100g of this sample with 1l of groundwater (10% solution). 500 drainage spray 1000ml per 1m ² dilution.

- about one trillion: except for the microbial components in the given sample (sterilized) 1000ml spray per 1m ² in a control sample was diluted with 500 Ground drainage.

- No processing sphere: 1000ml per spray groundwater 1m ^2.

(B) when and how often

The treatments were treated three times at 10 day intervals after the establishment.

(6) Cultivation Method

 Watering was conducted once a day at an average temperature of 28.9 ± 6.4 ℃ and humidity of 91.7 ± 8.2% in the plastic house. The fertilization rate in greenhouses was 1,500 kg per 10a and 17:17:21 kg of nitrogen: phosphoric acid: Kali. .

(7) Result investigation

(A) Growth analysis

-Ground biomass and ground dry weight

 10 weeks per repetition, the soil was completely removed from the lettuce and weighed as a top loading scale (minimum unit: 10mg), in the case of ground dry weight, measured after drying for 3 hours at 105 ℃.

-Underground biomass and underground dry weight

 Simultaneously with the above-ground analysis, the soil was completely removed from the lettuce by 10 weeks per repetition, and then the weight was measured as a top loading scale (minimum unit: 10 mg), and in the case of the underground dry weight, it was measured after drying for 3 hours at 105 ° C.

Leaf area measurement

Using a leaf area meter, the leaf area of the three largest mouths per repetition was measured and the average value was calculated.

-Investigation of root length

 The lettuce roots collected at each time were thoroughly washed with water to remove soil, and then the roots were stretched out side by side. The treatment effect of the samples was evaluated by quantifying as follows.

 Less than -4 cm: 1; 4-6 cm: 3; 6 cm or more: 3

-Number of leaves

 Ten weeks per repetition from the second treatment was investigated.

(B) Quality analysis

Lettuce Leaf Color Count

 From the second investigation period, 10 weeks per repetition was examined in multiple pipes. The treatment effect of the test sample was evaluated by quantification as follows.

1: When the area of less than 1/5 of the whole leaf is red

2: 1/5 to 3/5 of the entire leaf is red

3: 3/5 or more of the whole leaf is red

-Lettuce Leaf Texture Survey

From the second investigation period, 10 weeks per repetition was conducted. The treatment effect of the test sample was evaluated by quantification as follows.

1: surface of lettuce leaf is very rough

2: when the surface of lettuce leaves is medium trait

3: when the surface of lettuce leaves is soft

(8) soil analysis

The soil content of each treatment was collected and commissioned by Gangjin-gun Agricultural Technology Center to analyze the fertilizer content in the soil.

(9) soil microbial analysis

 After each treatment, 1 g of soil collected from each soil was treated in a sterile test tube containing 10 ml of sterilized water, mixed uniformly, and the number of soil microorganisms was examined by dilution flat culture. In particular, the effective microorganism of this test was determined by counting based on the shape and color of the colony.

I. Results and Discussion

(1) Analysis of principal components of test sample

(A) Analysis method

10 g of the sample was dissolved in sterile water and stirred for about 24 hours to completely elute the main component (microorganism), and then the filtrate was serially diluted by 10-fold, and then, respectively, in anaerobic conditions for 7 days in a selective medium for photosynthetic bacteria culture (PDA medium). After incubation, the bacterial density was examined by counting the total number of colonies (colonies).

(B) Analysis results

Effective microorganisms " Rhizopus oligosporus " were present in this sample at a density of 2.5 x 10 ⁶ cfu / g.

(2) Package test result

(A) Effect of test sample on growth of underground length

 Table 5 shows the results of investigating how the length of the underground growth of lettuce was promoted by the test sample treatment.

<Table 5> Test results for length growth of lettuce subterranean specimens

* The letters that appear after the mean and the error value are the results of comparisons between means using Duncan's multiple test (SAS Institute, 1988), indicating that the different characters are statistically significant (type 1 error a = 0.05). Means that.

As a result of investigating the root lengths of ground lettuce by treatment group and season, it was found that the treatment group was significantly superior to the control or untreated group during the investigation period. In other words, the length of lettuce roots was increased by about 14.4% after 10 days, about 16.4% after 20 days, and about 19.3% after 30 days. Is admitted.

On the other hand, the control group (sample treatment group excluding the microbial component from the test sample) did not show any difference from the untreated group in terms of root length, because it was considered that the role of the effective microorganism was not shown. It has been indirectly proven to promote this lettuce undergrowth.

(B) Effect of test sample on the living weight of underground

 Table 6 shows the results of investigating the increase in the underground live weight of lettuce by the test sample treatment.

<Table 6> Results of Effect Test on Underground Liver Weight of Lettuce Under Test Sample (Unit: g)

* The letters that appear after the mean and the error value are the results of comparisons between means using Duncan's multiple test (SAS Institute, 1988), indicating that the different characters are statistically significant (type 1 error a = 0.05). Means that.

As a result of the measurement of the underground weight of subcutaneous lettuce by season and season, it was found that the treatment group was heavier than the control or untreated group in the same period as the root length. In other words, the lettuce root weight of the treated group increased by 32.2% after 10 days, about 23.5% after 20 days, and about 28.9% after 30 days. Is admitted.

On the other hand, the control group (sample treatment group excluding the microbial component from the test sample) did not show a significant difference from the non-treated group in the root body, which may be because the effective microorganism role in the test sample could not be exerted.

(C) Effect of test sample on dry weight of underground part

Table 7 shows the result of investigating the increase in dry weight of lettuce under the test sample.

<Table 7> Effect test results on dry weight of lettuce underground samples (Unit: g)

* The letters that appear after the mean and the error value are the results of comparisons between means using Duncan's multiple test (SAS Institute, 1988), indicating that the different characters are statistically significant (type 1 error a = 0.05). Means that.

As a result of measuring the dry weight of the ground lettuce by the treatment group and the season, the treatment group, like root length and live weight, increased more than the control or untreated group. In other words, the dry weight of lettuce roots in the treatment group increased about 50.5% after 10 days, about 23.1% after 20 days, and about 25.0% after 30 days. The effect is recognized.

On the other hand, the control group (sample treatment group excluding the microbial component from the test sample) did not show any difference from the non-treated group in the root body, which is considered to be because the role of the effective microorganisms in the test sample could not be exerted.

(D) Effect of Test Samples on the Ground Live Weight

Table 8 shows the effects of the test samples on the fresh weight of lettuce.

<Table 8> Effect of Test Sample on Liver Weight of Lettuce (unit: g)

* The letters that appear after the mean and the error value are the results of comparisons between means using Duncan's multiple test (SAS Institute, 1988), indicating that the different characters are statistically significant (type 1 error a = 0.05). Means that.

The ground weights of the lettuce treated with the test samples were also significantly better than those of the control and the untreated, as in the case of the underground, and the effect was sustained throughout the investigation period. In other words, the raw ground weight of lettuce was increased by about 25.7% after 10 days, about 22.9% after 20 days, and about 23.9% after 30 days, compared to the control. It can be said. Therefore, it can be seen that the treatment of the present formulation enhances the growth of the above-ground part as well as the underground part of the lettuce. On the other hand, the control (treatment that excludes microorganisms from the test sample) does not show a significant difference from the non-treatment in terms of growth of lettuce ground.

(E) Effect of test sample on ground dry weight

Table 9 shows the result of investigating the increase in dry weight of the ground by the test sample.

<Table 9> Effect test results on dry weight of lettuce ground portion of test sample (unit: g)

* The letters that appear after the mean and the error value are the results of comparisons between means using Duncan's multiple test (SAS Institute, 1988), indicating that the different characters are statistically significant (type 1 error a = 0.05). Means that.

As a result of measuring the dry weight of ground lettuce at each treatment period and time period of the test sample, it was found that the treatment group increased as compared to the control or untreated group during the irradiation period. In other words, the amount of lettuce roots in the treatment group increased about 33.3% after 10 days, about 41.7% after 20 days, and about 18.8% after 30 days. It is admitted.

On the other hand, the control group (sample treatment group excluding microbial components from the test sample) did not show any difference from the untreated group in the dry weight of the ground part, which may be due to the inability of the effective microorganisms in the test sample.

(F) Effect of test sample on lettuce leaf area

Pulverized leaf area of lettuce, which is a factor related to the marketability of lettuce, was performed. The results are shown in Table 10.

<Table 10> Investigation results on the leaf area of lettuce (Unit: cm ² )

* The letters that appear after the mean and the error value are the results of comparisons between means using Duncan's multiple test (SAS Institute, 1988), indicating that the different characters are statistically significant (type 1 error a = 0.05). Means that.

The leaf area of the lettuce treated with the test sample was significantly better than that of the control or untreated group, and this effect was shown to continue until the harvest period after treatment. In other words, the lettuce leaf area of this sample was increased to about 20.0% after 10 days, about 20.3% after 20 days, and about 21.6% after 30 days, compared to the control, and the leaf area of this sample was increased. Can be admitted.

On the other hand, the control group showed similar results to the untreated group or slightly better than the untreated group, which is considered to be an effect of the organic matter (degreasing steel) contained in the control group. However, since the treatment showed a much better performance than the control, it is evident that the main cause of the leaf area increase effect is the microorganisms in the specimens.

(G) Effect of Test Sample on Lettuce Leaves

The lettuce leaves related to the yield of lettuce were analyzed and the results are shown in Table 11.

<Table 11> Investigation of the influence of lettuce samples on lettuce leaves

The number of leaves of seasoned lettuce was also higher than that of control or untreated. That is, the average number of treatments was 0.7 after 20 days and 0.8 after 30 days, compared to the control.

On the other hand, the control showed similar results to the untreated or slightly better than the untreated, which is considered to be the effect of the organic matter (degreasing steel) contained in the control. However, the treatment group showed significantly better results than the control group, so the main cause of the foliar increase effect is obviously the microorganisms in the test sample.

(H) Effect of Test Sample on Leaf Colors of Lettuce

Table 12 shows the results of analyzing the lettuce leaf color, which is a measure of treatment group, survey period, and qualitative merchandise.

<Table 12> Investigation of the influence of lettuce leaves on the sample color

Leaf color of the lettuce treated with the test sample was relatively better than that of the control. These effects continue to persist for the entire investigation period after 20 days of treatment. That is, when the microbial agent was treated, it was found that the tendency to redden the lettuce leaves, and therefore, it was confirmed that the quality and yield of lettuce were improved by treating the test sample.

(I) Effect of Test Sample on Lettuce Leaf Texture

Table 13 shows the results of analyzing the texture of lettuce leaves, which is a measure of the quality of lettuce.

<Table 13> Lettuce Leaf Texture Survey Results According to the Treatment of Specimen

The texture of the lettuce treated with the test sample was better than that of the control or the untreated. That is, when the test sample was treated, the lettuce leaves tended to be soft, whereas the control group excluding the active ingredient of the preparation did not show any difference compared to the untreated group.

All. Soil Microbial Density Change Analysis

(A) Effect of test sample on soil microbial density

 After the treatment of the test sample, the change of the microbial density of all fields was analyzed by fungal species (bacteria, fungi, actinomycetes), and the results are shown in Table 14. Treatment and data analysis were performed in three replicates.

<Table 14> Changes in soil microorganisms after treatment of test samples (Unit: 10 ⁶ cfu / g)

As a result of analysis of soil microbial density after the test sample, it can be seen that each species shows characteristic density change pattern over time. That is, in the case of bacteria, the control denotes a density of 369-381 x 10 ⁶ cfu / g throughout the analysis period, while the untreated throughout the analysis period represents a density of 365-385 x 10 ⁶ cfu / g, given sample treatment In this case, the density is 485-492 x 10 ⁶ cfu / g throughout the test period.

In the case of fungi, the control showed a density of 5.9-7.3 x 10 ⁶ cfu / g throughout the analysis period, and the untreated group showed a density of 6.3-7.1x10 ⁶ cfu / g, whereas the test sample was tested. A density of 8.9-9.3 × 10 ⁶ cfu / g is shown throughout the period.

The results showed that there was almost no difference in density between the control and the non-treated sections for each species, and the density of microorganisms was higher in the control sample than in the control. That is, during the investigation period, the bacteria showed a 28.1 · 33.3% increase in density compared to the control, and in the case of the radiation anew, it showed a 78.9-104.1% increase in density, whereas the fungus showed a 27-38% increase in density. Density increase is shown. These trends appeared to be nearly the same throughout the investigation.

That is, as the sample was treated, the density of bacteria, actinomycetes, and mold in the soil increased, which is considered to be an effect of effective microorganisms in the sample. The growth of effective microorganisms increased the density of bacteria, and the increase of bacterial density indirectly caused an increase in actinomycetes density. This is due to the increase in root surface area.

In addition, the density of mold increased by about 38% during the first irradiation period, showing only a 2% density increase until the third irradiation period. It means that it is maintained as it is.

(B) Analysis of dynamics in soil of effective microorganisms in test samples

Table 15 shows the results of analyzing the dynamics (density change, etc.) in soil of effective microorganisms ( Rhizopus oligosporus ) after the test sample. Effective microorganisms were examined by distinguishing the colony's shape, color, and microscopic features.

<Table 15> Dynamic analysis of the main sample ( Rhizopus sp . JK 2213) (Unit: 10 ⁶ cfu / g)

Results of soil- density investigation of the main sample ( Rhizopus oligosporus ) were 0.91 x 10 ⁶ cfu / g after 2 days of treatment, 7.4 x 10 ⁶ cfu / g after 5 days of treatment, and 12.7 x 10 ⁶ cfu / after 10 days of treatment From g after 30 days of treatment, the density is 11.6-13.2 x 10 ⁶ cfu / g. That is, the main component (effective microorganism) was settled in the soil in about 10 days after treatment, and the density was maintained until the 30th day thereafter. In conclusion, it can be seen that the growth promoting effect of the lettuce is due to the growth of the effective microorganisms treated.

<Example 3> Confirmation of the plant disease lowering effect of the plant

end. Materials and methods

(1) Cultivation test site

 Yeongdong Farm, 95 Beoljeong-ri, Sinjeon-myeon, Gangjin-gun, Jeollanam-do South Korea

(2) disclosure crops

Chucheong cucumber

(3) Test method

 3 kg per 10a of the microbial preparations according to the present invention were treated 4 days before the plantation of Chucheong cucumbers, and the control was treated with a sample (sterilization treatment) except for the microorganisms according to the present invention. 2 kg of microbial agents were administered per 10a at 15-day intervals and the incidence of Downy mildew was compared.

(4) results

In the control group, a large amount of Mycelial disease occurred, whereas in the group treated with the microbial agent according to the present invention, the occurrence of Mycelial disease was suppressed. Figure 2 shows the difference in the degree of the occurrence of downy mildew in the group (A) treated with the microbial agent according to the present invention and the group (B) not treated with the microbial agent of the present invention. In addition, the yield was increased by 46.7% in the group treated with the microbial agent of the present invention compared to the control.

The novel Lysopus oligosporus KCCM 10624 according to the present invention promoted the growth of plants. The results of the cultivation test showed that the treatment area increased 23.5% -32.3%, 22.9-25.7%, and 20.0-21.6%, respectively, in the ground growth, ground growth, and leaf area, which were related to the yield. The increase was 0.7-0.8 compared to the above. On the other hand, Rhizopus sp. Soil density of KCCM 10624 also increased continuously throughout the test period. As a result of soil microbial analysis, bacteria showed 28.1-33.3% increase in density compared to the control, and actinomycetes showed 78.9-104.1% increase in density compared to the control, and mold also showed 27.0-38.0. A density increase of% was shown. In other words, the afl degree of bacteria and actinomycetes increased and the density of mold also increased by the treatment of the test sample. In view of the soil microbial density change, it is determined that the soil's biomass is generally improved, and thus, the soil is improved by the treatment of the microbial agent according to the present invention.

In addition, the lettuce leaf color and texture, which is an item related to the merchandise, were also examined, and it was confirmed that the test strips treated with the microbial agent according to the present invention in both tests were improved in the commerciality as compared to the control or the untreated strip.

In addition, the microbial preparations according to the present invention were found to have no harmful properties to crops.

In conclusion, through the cultivation test, it was confirmed that the microbial agent according to the present invention enhances the growth of the starting material (lettuce), the value as a fertilizer is sufficient.

Lysopus oligosporus KCCM 10624 according to the present invention increased the resistance of plant diseases of plants.

Claims (8)

New strain Lysopus oligosporus with growth promoting effect of lettuce and cucumber KCCM 10624. Pure culture of claim 1 strain. The growth promoter of the lettuce containing the strain of claim 1 or the pure culture of claim 2 as an active ingredient. A method of promoting growth of lettuce by spreading the effective amount of the growth promoter of claim 3 onto the lettuce. delete A disease resistance enhancer of cucumber containing the strain of claim 1 or the pure culture of claim 2 as an active ingredient. A method of improving cucumber disease resistance by spraying cucumbers with an effective amount of the disease resistance enhancer according to claim 6. delete

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