WO2004090143A3 - Modulation de l'activite des cytokinines dans les plantes - Google Patents

Modulation de l'activite des cytokinines dans les plantes Download PDF

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Publication number
WO2004090143A3
WO2004090143A3 PCT/US2004/010064 US2004010064W WO2004090143A3 WO 2004090143 A3 WO2004090143 A3 WO 2004090143A3 US 2004010064 W US2004010064 W US 2004010064W WO 2004090143 A3 WO2004090143 A3 WO 2004090143A3
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WO
WIPO (PCT)
Prior art keywords
plant
plants
heterologous nucleotide
expression
nucleotide sequence
Prior art date
Application number
PCT/US2004/010064
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English (en)
Other versions
WO2004090143A2 (fr
Inventor
Jeffrey E Habben
Chris Zinselmeier
Dwight T Tomes
Shane E Abbitt
Timothy G Helentjaris
Xiaomu Niu
Original Assignee
Pioneer Hi Bred Int
Jeffrey E Habben
Chris Zinselmeier
Dwight T Tomes
Shane E Abbitt
Timothy G Helentjaris
Xiaomu Niu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Jeffrey E Habben, Chris Zinselmeier, Dwight T Tomes, Shane E Abbitt, Timothy G Helentjaris, Xiaomu Niu filed Critical Pioneer Hi Bred Int
Priority to CA2521497A priority Critical patent/CA2521497C/fr
Priority to EP04749636A priority patent/EP1613754A2/fr
Priority to BRPI0409178-7A priority patent/BRPI0409178A/pt
Priority to AU2004227360A priority patent/AU2004227360B2/en
Priority to NZ543070A priority patent/NZ543070A/en
Publication of WO2004090143A2 publication Critical patent/WO2004090143A2/fr
Publication of WO2004090143A3 publication Critical patent/WO2004090143A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0032—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with oxygen as acceptor (1.5.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention porte, en général, sur la biologie moléculaire des plantes, et plus spécifiquement sur des procédés et sur des réactifs de l'expression à régulation temporelle et/ou spatiale des gènes qui affectent des taux métaboliquement effectifs des cytokinines dans les plantes, notamment dans les graines et les tissus reproducteurs connexes des plantes femelles. Cette invention porte également sur des plantes transgéniques ayant des taux accrus de l'expression des cytokinines, la plante transgénique présentant des caractéristiques utiles telles que l'augmentation de la grosseur des graines, la diminution de l'avortement de l'amande d'extrémité, l'augmentation de la grenaison sous des conditions environnementales défavorables ou la stabilité de la production. La présente invention porte également sur des compositions et sur des procédés de régulation de l'expression des séquences nucléotidiques hétérologues dans une plante. Les compositions comprennent de nouvelles séquences nucléotidiques pour les promoteurs préférés connus de graines tels que eep1 et eep2. L'invention porte aussi sur un procédé d'expression d'une séquence nucléotidique hétérologue dans une plante utilisant les séquences du promoteur. Le procédé consiste à transformer une cellule de plante de sorte qu'elle comprenne une séquence nucléotidique hétérologue liée de manière fonctionnelle à l'un des promoteurs de l'invention et à régénérer une plante à transformation stable à partir de la cellule transformée.
PCT/US2004/010064 2003-04-04 2004-04-02 Modulation de l'activite des cytokinines dans les plantes WO2004090143A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA2521497A CA2521497C (fr) 2003-04-04 2004-04-02 Modulation de l'activite des cytokinines dans les plantes
EP04749636A EP1613754A2 (fr) 2003-04-04 2004-04-02 Modulation de l'activite des cytokinines dans les plantes
BRPI0409178-7A BRPI0409178A (pt) 2003-04-04 2004-04-02 método para produzir plantas transgênicas e para modular a atividade de citocina em plantas, plantas transgênicas, dna recombinate, promotor e cassete de expressão
AU2004227360A AU2004227360B2 (en) 2003-04-04 2004-04-02 Modulation of cytokinin activity in plants
NZ543070A NZ543070A (en) 2003-04-04 2004-04-02 Modulation of cytokinin activity in plants

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46071803P 2003-04-04 2003-04-04
US60/460,718 2003-04-04

Publications (2)

Publication Number Publication Date
WO2004090143A2 WO2004090143A2 (fr) 2004-10-21
WO2004090143A3 true WO2004090143A3 (fr) 2005-05-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/010064 WO2004090143A2 (fr) 2003-04-04 2004-04-02 Modulation de l'activite des cytokinines dans les plantes

Country Status (9)

Country Link
EP (1) EP1613754A2 (fr)
CN (3) CN101220357A (fr)
AR (1) AR045420A1 (fr)
AU (1) AU2004227360B2 (fr)
BR (1) BRPI0409178A (fr)
CA (1) CA2521497C (fr)
NZ (1) NZ543070A (fr)
WO (1) WO2004090143A2 (fr)
ZA (1) ZA200508006B (fr)

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EP1520028A4 (fr) * 2002-06-19 2006-08-16 Basf Plant Science Gmbh Compositions et procedes pour ameliorer les performances des plantes
EP1741788A1 (fr) * 2005-07-06 2007-01-10 Freie Universität Berlin Plante transgénique ayant une activité des récepteurs aux cytokinines réduite de manière sélective dans certains tissus
WO2007027866A2 (fr) * 2005-08-30 2007-03-08 Monsanto Technology Llc Plantes transgeniques a traits agronomiques ameliores
US8431775B2 (en) 2008-12-04 2013-04-30 Pioneer Hi Bred International Inc Methods and compositions for enhanced yield by targeted expression of knotted1
CA2765034A1 (fr) 2009-06-09 2010-12-23 Pioneer Hi-Bred International, Inc. Promoteur d'endosperme precoce et procedes d'utilisation
US8440891B2 (en) 2009-09-22 2013-05-14 Board of Trustees of the University of Akransas, N.A. Rice cultivar CL 142-AR
US8440892B2 (en) 2009-10-15 2013-05-14 Board Of Trustees Of The University Of Arkansas, N.A. Rice cultivar CL 181-AR
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PL2512226T3 (pl) * 2009-12-17 2019-10-31 Pioneer Hi Bred Int Modyfikacja DP-004114-3 kukurydzy i sposoby jej wykrywania
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WO2013096810A1 (fr) 2011-12-21 2013-06-27 The Curators Of The University Of Missouri Variété de soja s05-11482
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Publication number Publication date
CN101220357A (zh) 2008-07-16
EP1613754A2 (fr) 2006-01-11
NZ543070A (en) 2008-10-31
CA2521497A1 (fr) 2004-10-21
AR045420A1 (es) 2005-10-26
ZA200508006B (en) 2007-01-31
AU2004227360A1 (en) 2004-10-21
WO2004090143A2 (fr) 2004-10-21
AU2004227360B2 (en) 2009-05-28
CA2521497C (fr) 2012-11-27
CN1798843A (zh) 2006-07-05
BRPI0409178A (pt) 2006-05-02
CN101220358A (zh) 2008-07-16

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