WO2004078225A1 - 羊膜由来医用材料、及びその作製方法 - Google Patents
羊膜由来医用材料、及びその作製方法 Download PDFInfo
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- WO2004078225A1 WO2004078225A1 PCT/JP2004/001692 JP2004001692W WO2004078225A1 WO 2004078225 A1 WO2004078225 A1 WO 2004078225A1 JP 2004001692 W JP2004001692 W JP 2004001692W WO 2004078225 A1 WO2004078225 A1 WO 2004078225A1
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- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/40—Preparation and treatment of biological tissue for implantation, e.g. decellularisation, cross-linking
Definitions
- the present invention relates to an amniotic membrane-derived medical material and its use.
- the medical material of the present invention can be used, for example, as a wound filling material, a wound protecting material, a wound healing material, an adhesion preventing material, an artificial tissue, and the like. It can be used for producing endothelium (for example, corneal endothelium).
- tissue for transplantation there is an attempt to reconstruct the skin epidermis and corneal epithelium on an appropriate substrate in vitro and use this as tissue for transplantation.
- the base material used for constructing such a tissue for transplantation has a high affinity (and low immunogenicity) for a living body, and a desired cell adheres well thereon and proliferates. Characteristics are required.
- amniotic membrane has been widely used as a material having such characteristics.
- Amniotic membrane is considered to be particularly preferable as a material for preparing grafts because it is a membrane that wraps a fetus together with amniotic fluid and has low immunogenicity.
- the amniotic membrane has a structure in which an epithelial layer is formed on a parenchymal layer composed of collagen in brief via a basement membrane.
- amniotic membrane has low immunogenicity, but in order to further reduce immunogenicity, and when constructing a tissue layer for transplantation by constructing a cell layer thereon, cells derived from amniotic membrane are used. Therefore, it is considered that it is preferable to remove the epithelial layer before use (see Japanese Patent Application Laid-Open No. 5-56987).
- the collected amniotic membrane is washed to remove any extraneous matter, and then the epithelial layer is peeled off.
- the epithelium-free amniotic membrane thus obtained is stored refrigerated or frozen until use, except when used immediately after preparation. Therefore, sufficient temperature control before use was required, and its handling (especially during transportation) required special care. Further, since it is a material to be implanted in a living body, it is necessary to perform a sufficient sterilization treatment before use.
- ⁇ -rays were applied to an epithelium-free amniotic membrane in a refrigerated or frozen state.
- discoloration and the like of the solution were observed by performing electron beam treatment or the like, and the solution was not able to withstand the required sterilization treatment.
- the present invention has been made in view of the above problems, and has as its main objects easy handling, sufficient sterilization treatment, and a good base material for constructing a cell layer thereon.
- Another object of the present invention is to provide an amniotic membrane-derived medical material that functions.
- the present inventors focused on the basement membrane of the amniotic membrane, and found that it is important to leave the basement membrane and maintain its structure in order to make it a suitable medical material as a base material for constructing a cell layer. Thought. From the viewpoint of handling, it was considered that the dried form was most preferable before use. Based on the above idea, the present inventors have attempted to construct a medical material that can achieve the above-mentioned object using amniotic membrane as a starting material.
- the epithelium of the amniotic membrane was removed so that at least part of the basement membrane remained. Subsequently, moisture was removed by vacuum freeze-drying to obtain a dried amniotic membrane (amniotic membrane containing no epithelium).
- a transplant experiment was performed using the finally obtained cell layer (corneal epithelium-like sheet) as a graft. As a result, it was confirmed that the graft was securely attached to a living body and a good corneal reconstruction effect was obtained.
- the present invention has been made based on the above results, and provides the following configurations.
- Amnion-derived medical material having the following properties (1) to (3): (1) in a dry state;
- amniotic membrane-derived medical material according to [1], further comprising the following property (4): (4) The amniotic membrane-derived medical material is housed in a container substantially without contact with oxygen.
- amniotic membrane-derived medical material according to [1] or [2], wherein the state in which there is substantially no contact with oxygen is a state in which the inside of the container is evacuated or replaced with nitrogen.
- amniotic membrane-derived medical material according to any of [1] to [3], wherein the cells are corneal epithelial cells, corneal endothelial cells, or oral mucosal epithelial cells.
- a method for producing an amniotic membrane-derived medical material comprising the following steps (i) and (i0):
- step (i) removing the epithelial layer from the amniotic membrane, leaving at least a portion of the basement membrane; and (ii) maintaining the remaining basement membrane in use so that cells can adhere and grow thereon. Drying the epithelium-free amniotic membrane obtained in step (i).
- step (iiii) a step of storing the dried body obtained in the step (ii) in a container so as to be substantially free from contact with oxygen.
- a method for producing a corneal epithelium-like sheet comprising the following steps:
- a step of culturing corneal epithelial cells on the medical material according to any one of [1] to [4].
- a method for producing a corneal epithelium-like sheet comprising the following steps (a) and (b):
- the step (a) is carried out in the presence of a supporting cell and in the presence of a pore-size separator between the supporting cell and the medical material, through which the supporting cell cannot pass.
- a method for producing a corneal endothelium-like sheet comprising the following steps (A) to (C):
- (B-1) a step of increasing the cell density of the cell suspension by centrifugation.
- step (C) includes the following steps (C-1) to (C-5):
- (C-2) a step of placing the medical material according to any one of [1] to [4] on the bottom surface of the container; (C-3) seeding the cell suspension on the medical material;
- corneal epithelial cells as used herein is used as a generic expression of the cells contained in the corneal epithelial cell layer, that is, also includes corneal epithelial stem cells.
- corneal endothelial cell is used as a generic expression of cells contained in the corneal endothelial cell layer, that is, includes corneal endothelial stem cells.
- corneal epithelium-like sheet is used as a term meaning a cell layer having characteristics similar to the corneal epithelium and capable of being subjected to transplantation in place of the corneal epithelium.
- corneal epithelium J is used as a term meaning a composition having a cell layer having characteristics similar to that of the corneal epithelium and capable of being subjected to transplantation for reconstruction of the corneal epithelium.
- corneal endothelium-like sheet is used as a term meaning a cell layer that has characteristics similar to corneal endothelium and can be used for transplantation in place of corneal endothelium.
- sheet for transplanting corneal endothelium is used as a term meaning a composition having a cell layer having characteristics similar to corneal endothelium and which can be subjected to transplantation for reconstruction of corneal endothelium.
- FIG. 1 is a diagram showing an amnion image by a scanning electron microscope.
- A is an image of the amniotic membrane surface in a normal state (without epithelial treatment)
- B is an image of the amniotic membrane surface after the epithelial treatment (0.02% EDTA solution).
- A polygonal amniotic epithelium is observed.
- no amniotic epithelium was observed in B, confirming that the epithelium was completely removed.
- Figure 2 is a table summarizing the results of surface analysis of freeze-dried amniotic membrane. In the table, + indicates positive, and 1 indicates negative.
- AM (-) FD T ray dried amniotic membrane obtained by the method of Example 5, AM (+): a sample that was collected and washed only, AM (-): an epithelium was removed, and freeze-drying was performed. Samples not done, AM (-) dispase: A sample without epithelial removal by dispase treatment and without drying, AM (-) FD: A sample with epithelial removal and lyophilization but not sterilization, AM (-) ⁇ ray: A sample that has been sterilized without lyophilization after removal of the epithelium.
- FIG. 3 is a cross-sectional view schematically showing the state of instruments and the like when culturing oral mucosal epithelial cells on amniotic membrane.
- FIG. 4 is a diagram showing a corneal epithelial cell layer formed on a freeze-dried amniotic membrane (HE (hematoxylin-sin) -stained image).
- HE hematoxylin-sin
- FIG. 5 shows the state of the ocular surface before surgery (upper column) and the state of the ocular surface (fluorescein-stained image) two days after transplantation of the sheet for corneal epithelial transplantation (lower column). ). Portions stained with full-age restain (arrows) are indicated by oblique lines. The ocular surface (transplant) was not stained with full-year-old restain, indicating that the graft had survived on the ocular surface. In addition, fluorescein staining was observed all around the graft.
- a first aspect of the present invention relates to an amniotic membrane-derived medical material, which has the following properties (1) to (3).
- the medical material of the present invention is firstly characterized by being derived from amniotic membrane. "Derived from amniotic membrane” means that it is obtained from amniotic membrane as a starting material. From the viewpoint of immunogenicity, the medical material of the present invention is preferably derived from human amniotic membrane.
- the human amniotic membrane is a membrane covering the outermost layers of the uterus and placenta, and is composed of a collagen-rich parenchymal tissue formed with a basement membrane and an epithelial layer. Since the medical material of the present invention has the property (1), its handling becomes easy. Specifically, it can be stored at room temperature (eg, about 10 ° C to about 35 ° C).
- the property (2) of the present invention contributes to a reduction in immunogenicity. That is, amniotic membrane is originally a material having low immunogenicity, but the immunogenicity is further reduced by completely removing the epithelial layer. On the other hand, the property of not having an epithelial layer is important in that a desired cell layer can be favorably constructed on the medical material of the present invention.
- amniotic membrane with the remaining epithelial layer is used as a base material and specific cells are seeded thereon to construct a cell layer, the growth of the seeded cells is inhibited by the amniotic epithelial cells.
- amniotic epithelial cells increase There is no fear of breeding inhibition.
- the medical material of the present invention having such a feature of having no epithelium functions as a base material suitable for constructing a target cell layer.
- the medical material of the present invention having the property (3) makes it a material that can be suitably used as a base material for constructing a target cell layer.
- Amniotic membranes that have been completely air-dried after complete removal of the epithelium i.e., naturally dried amniotic membranes consisting of only the parenchymal layer, generally have the basement membrane components degraded, and when cells are seeded thereon, the cells adhere well. do not do.
- the basement membrane since the basement membrane remains and its structure is highly maintained, the cells seeded thereon adhere well by the adhesion factor contained in the basement membrane, and as a result, Cell proliferation and stratification occur.
- “when used” in the properties of (3) means that the medical material of the present invention is immersed in an appropriate solution (sterilized water, physiological saline, phosphate buffer, an appropriate medium, etc.) and restored from a dry state. What did you do when you let me.
- the presence or absence of the basement membrane can be confirmed by examining the presence of collagen IV ( ⁇ 1, ⁇ 2, and ⁇ 5), collagen VI and laminin 5, which are characteristic components of the basement membrane. That is, in the medical material of the present invention, at the time of restoration, at least one, preferably a plurality, or even all of these components are detected.
- the detection of collagen IV and the like can be performed by an immunostaining method using an antibody specific to these.
- Examples of cells grown on the medical material of the present invention include epithelial cells (corneal epithelial cells, skin epidermal cells, oral mucosal epithelial cells, etc.) and endothelial cells (corneal endothelial cells, etc.).
- the oral mucosal epithelial cells include, for example, cells present in the tooth root (oral mucosal epithelial cells), mucous epithelial cells in the lips, mucosal epithelial cells in the palate, and mucosal epithelial cells in the upper part.
- the amniotic membrane-derived medical material of the present invention is an extremely useful material for constructing a cell layer (tissue) on the premise of transplantation. It is preferable that the medical material of the present invention is provided in a state of being practically not in contact with oxygen, housed in a container. In this mode, quality is not deteriorated by oxygen, and high quality can be maintained for a long period of time.
- “A state in which there is substantially no contact with oxygen” refers to a state in which there is substantially no oxygen in the container, and specifically, a state in which the container is evacuated or the container is filled with nitrogen ( (Substituted with nitrogen).
- the “container” is not particularly limited as long as it is suitable for storing the medical material of the present invention. For example, a bag-like or tube-like one made of a plastic synthetic resin (two sheets are superposed to seal the peripheral portion thereof) Or a bottle made of an inorganic material such as glass can be used as the container of the present invention.
- a second aspect of the present invention relates to a method for producing an amniotic membrane-derived medical material, and comprises the following steps (i) and (i i).
- step (ii) drying the epithelium-free amniotic membrane obtained in step (i), provided that the remaining basement membrane retains a structure on which it can adhere and proliferate cells when used.
- step (i) the epithelial layer is removed.
- the basement membrane is not removed at the same time, but at least a part thereof is left.
- Such a process is performed by, for example, removing the epithelial layer using a cell scraper, tweezers, or the like.
- the adhesion between the cells constituting the epithelial layer is loosened in advance with EDTA or a proteolytic enzyme or the like, and then the cell is scraped with a cell scraper or the like.
- a pretreatment treatment with EDTA or the like
- step (i) of the present invention is to completely remove the epithelial layer while leaving at least a part of the basement membrane while maintaining its original function.
- pretreatment with EDTA eg, adding EDTA to a concentration of 0.02% to 0.1% and reacting at 4 ° C to 37 ° C for 1 hour to 4 hours
- EDTA eg, adding EDTA to a concentration of 0.02% to 0.1% and reacting at 4 ° C to 37 ° C for 1 hour to 4 hours
- the amniotic membrane used in step (i) is preferably a human amniotic membrane.
- Human amniotic membrane can be collected, for example, from human fetal membrane, placenta, etc. obtained after birth during parturition.
- a human amniotic membrane can be prepared by treating and purifying an integrated body consisting of a human fetal membrane, placenta, and umbilical cord obtained immediately after parturition.
- known methods such as the method described in JP-A-5-56987 can be employed.
- the amniotic membrane is peeled from the fetal membrane obtained at the time of parturition, the remaining tissue is removed by a physical treatment such as ultrasonic cleaning, and an enzymatic treatment, and the human amniotic membrane can be prepared through an appropriate washing step.
- the human amniotic membrane thus prepared can be frozen and stored until just before step (i).
- the freezing of human amniotic membrane can be carried out, for example, at -80 ° C in a liquid in which DMEM (Dulbecco's modified Eagle's medium) and glycerol are mixed in an equal volume ratio. Cryopreservation not only improves operability, but also reduces antigenicity.
- step (ii) which is performed subsequent to step (i)
- the epithelium-free amniotic membrane obtained in step (i) retains a structure that allows the remaining basement membrane to adhere and grow cells thereon when in use. Dry under all conditions.
- the method of the drying treatment is not limited as long as the treatment can be performed under such conditions, but a freeze-drying treatment is preferably employed.
- the sample is generally frozen and solidified in a low-pressure environment (vacuum) with a boiling point of about -20 ° C (107 Pa, 0.8 Torr) to about -50 ° C (4 Pa, 0.03 Torr). (For example, frozen at about -40 ° C), the moisture is removed by sublimation.
- freeze-drying treatment it is possible to uniformly dehydrate from the inside, and since a high degree of drying is realized, drying can be performed while maintaining the original machine food and form at a high level.
- freeze The coagulation and drying treatment is as follows: 1. Less deterioration during processing, 2. Easy sterilization, 3. Dry body with excellent resilience, 4. Dry body with excellent storage stability, It has features such as.
- the freeze-drying process can be performed by a freeze-dryer equipped with a vacuum chamber, a cooling / heating device, and an exhaust device (cold trap and vacuum pump). Numerous freeze-drying apparatuses are commercially available, and in step (ii) of the present invention, any one selected from these can be used.
- the processing conditions can be set based on the instruction manual attached to the device used. At that time, the size of the sample to be subjected to the drying treatment, the degree of drying, and the like can be considered.
- the degree of drying can be set, for example, such that the water activity (AW) is less than 0.5. It is preferable to perform the following step (iii) after the above step (ii).
- step (ii) The step of storing the dried body obtained in the step (ii) in a container so that the dried body is substantially free from contact with oxygen.
- Step (iii) a dried epithelium-free amniotic membrane packaged in a state substantially shielded from oxygen is obtained.
- This provides an amniotic membrane-derived medical material that can be stored for a long period of time.
- Step (iii) can be carried out, for example, by placing the dried product obtained in step (ii) in a suitable container and then evacuating the container or replacing the container with nitrogen. Alternatively, it can be carried out by enclosing a deoxidizer in a container.
- the container may be, for example, a bag-shaped or tube-shaped container made of a plastic synthetic resin (the two sheets may be overlapped and the peripheral portion thereof may be sealed), or an inorganic material such as glass.
- a bottle-like material made of a material can be used.
- Another aspect of the present invention relates to a method for using the amniotic membrane-derived medical material provided in the first aspect of the present invention, and one embodiment thereof is a method for producing a corneal epithelium-like sheet.
- cells having the ability to differentiate into corneal epithelial cells or corneal epithelial cell-like cells on amniotic membrane-derived medical material (hereinafter collectively referred to as “corneal epithelial cells” Cells called “membrane epithelium”) are cultured. Corneal epithelial cells are obtained from the cornea of a suitable donor. If available, it is preferred to use the recipient's own corneal epithelial cells.
- corneal epithelium-like sheet that does not have a risk of immune rejection when subjected to transplantation can be produced, and thereby a transplantation operation without immune rejection can be performed.
- corneal epithelial cells other than the recipient can be used. In this case, the donor should be selected in consideration of immunocompatibility. Is preferred.
- corneal epithelial cell-like cells refers to cells capable of constructing a corneal epithelial-like cell layer in a living body by culturing in vitro or after transplantation.
- the “corneal epithelium-like cell layer” refers to various properties required to function as a corneal epithelium (for example, having high transparency, that the cells in the top layer are not keratinized, Function, etc.).
- oral mucosal epithelial cells have the ability to differentiate into such corneal epithelial cell-like cells (see PCT / JP02 / 1857). It is suggested that stem cells exist in the oral mucosal epithelium, and it is considered that differentiation can be easily induced into cells forming an epithelial-like cell layer.
- the use of oral mucosal epithelial cells means that it is easy to collect, a large amount of cells can be collected, and even when treating patients with binocular disease, preparing transplantation materials using their own cells It has advantages such as being able to do so. In particular, the advantage that transplantation materials derived from autologous cells can be applied to patients who cannot collect corneal epithelial cells is expected to greatly eliminate the problem of rejection, which is extremely important clinically.
- oral mucosal epithelial cells cells present in the tooth root (oral mucosal epithelial cells), lip cells, palate cells, and ⁇ cells are used. Above all, it is particularly preferable to use oral mucosal epithelial cells because of their high proliferation ability and low antigenicity. Oral mucosal epithelial cells are collected by excising or scraping the area where the target cells are located with a scalpel, etc. Can be In the oral mucosal epithelial cells, the oral mucosal epithelium adhering to the tooth extraction can be separated from the enamel cement transition part and collected from the obtained tissue piece. In order to remove impurities such as connective tissue, it is preferable to perform a treatment with an enzyme such as disperse tribune and a filter treatment.
- an enzyme such as disperse tribune and a filter treatment.
- Oral mucosal epithelial cells collected from the oral cavity of a patient other than the patient into which the corneal epithelium-like sheet prepared according to the present invention is transplanted can be used, but in consideration of immune rejection, oral mucosal epithelial cells can be used from the patient's own oral cavity. Is preferably collected and used for culture.
- the cells for corneal epithelium are cultured on the amniotic membrane-derived medical material of the present invention. That is, the collected cells for corneal epithelium are seeded on amniotic membrane-derived medical material and cultured under appropriate culture conditions.
- the cells for corneal epithelium have a cell density of about 1 ⁇ 10 3 cells / cm 2 or more, preferably about 1 ⁇ 10 3 cells / cm 2 to about 1 ⁇ 10 7 cells / cm 2 , and more preferably about 1 ⁇ 10 3 cells / cm 2. It can be seeded on amniotic membrane-derived medical material so as to have a density of 10 4 cells / cm 2 to about 1 ⁇ 10 6 cells / cm 2 .
- Culture of the cells for corneal epithelium is preferably performed in the presence of supporting cells.
- the supporting cells are also called feeder cells and supply growth factors and the like to the culture solution. By culturing in the presence of supporting cells, improvement in the proliferation efficiency of corneal epithelial cells can be expected.
- 3T3 cells such as Swiss mouse 3T3 cells, mouse NI H3T3 cells, and 3T3J2 cells
- mouse NI H3T3 cells are preferably used as supporting cells from the viewpoints of proliferation efficiency, ease of handling, and the like.
- mitomycin C or the like it is preferable to inactivate the supporting cells in advance using mitomycin C or the like. This is to prevent the proliferation of the cells for corneal epithelium from being inhibited by the proliferation of the support cells themselves, and to increase the proliferation efficiency of the cells for corneal epithelium. Such inactivation can also be performed by radiation treatment or the like.
- the pore-size separator When culturing the cells for corneal epithelium in the presence of the support cells, it is preferable to provide a pore-size separator between the support cells and the collagen layer, the pore-size separator not allowing the passage of the support cells.
- this isolating membrane By using this isolating membrane, it is possible to prevent feeder cells from entering the amniotic membrane-derived medical material side (ie, the corneal epithelial cell side) during culture. As a result, there is no possibility that supporting cells are mixed in the finally obtained corneal epithelium-like sheet. This means that a corneal epithelium-like sheet without the problem of immune rejection by supporting cells can be produced, and is clinically extremely significant.
- separator a known separator having a pore size that does not allow passage of the supporting cells can be appropriately selected and used.
- a membrane made of polycarbonate and having a pore size of about 0.4 jCim to 3.0 m can be used.
- the material of the separator is not particularly limited, and may be polyester or the like in addition to polycarbonate. Such separators are commercially available and can be easily obtained.
- the following method can be mentioned as an example of a culture method using an isolation membrane.
- the inactivated supporting cells are seeded in a container such as a dish (first container) and cultured to form a supporting cell layer on the surface of the container.
- a second container having a bottom surface formed of a separator is placed in the first container.
- the position of the second container is adjusted so that the bottom surface of the second container is in the culture solution.
- the amniotic membrane-derived medical material is placed on the bottom surface of the second container, that is, on the separator.
- cells for corneal epithelium are seeded on the amniotic membrane-derived medical material and cultured.
- the amniotic membrane-derived medical material is placed on the bottom surface of the second container in advance (a drying process may be performed after the placement), and the second container is placed in the first container seeded with the supporting cells. It may be placed, and cells for corneal epithelium may be seeded on amniotic membrane-derived medical material and cultured.
- Cell density of feeder cells for example, about 1 X 1 0 2 or ZCM 2 or more, preferably about 1 X 10 2 pieces / cm 2 ⁇ about 1 X 1 0 7 atoms cm 2, more preferably about 1 X 1 0 3 can be pieces / cm 2 ⁇ about 1 X 1 0 5 cells / cm 2.
- the number of support cells used is, for example, 1/10 3 times ⁇ 1 X 10 2 times Te, preferably can be determined promptly by using an incubation in such conditions the 0 2 to 1 times 1 / I. If the number of supporting cells is small, the proliferation rate of the cells for corneal epithelium decreases, and if the number is too small, good stratification of cells for corneal epithelium cannot be obtained. On the other hand, if the number of supporting cells is too large, the proliferation rate of corneal epithelial cells will be reduced, which is not preferable.
- the culture solution used for culturing the cells for corneal epithelium is not particularly limited as long as the cells can be grown and stratified.
- DMEM Dulbecco's modified Eagle's medium
- Ham's F12 medium Ham's F12 medium
- FBS 50%, insulin
- cholera toxin 0.1 nM
- epidermal growth factor EGF
- penicillin-streptomycin 50 IU / ml
- a mixed medium of DMEM and Ham F12 medium may be added.
- triiodothyronine for example, 2 nM
- glumin for example, 4 mM
- transranferin for example, 5 mg / ml
- adenine for example, 0.18 mM
- / or hydrid cortisone for example, 0.4 mg / ml
- step (b)) of bringing the surface layer of the layered cell layer into contact with air is performed to differentiate the cells forming the cell layer and to induce the barrier function.
- This step is also referred to herein as air-lifting.
- the surface of the culture solution is lowered by temporarily removing a part of the culture solution using a spot, a pipe, or the like, and thus the outermost layer of the cell layer is temporarily removed.
- Exposure can be performed by exposing it outside the culture solution. Alternatively, it can be carried out by lifting the cell layer together with the amniotic membrane-derived medical material and temporarily exposing the outermost layer from the surface of the culture solution.
- a tube The step may be performed by sending air into the culture solution using any method and bringing the air into contact with the uppermost layer of the cell layer. From the viewpoint of ease of operation, it is preferable to perform the method by lowering the surface of the culture solution to expose the outermost layer of the cell layer.
- the time for performing the step (b), that is, the time for bringing the outermost layer of the layered cell layer into contact with air varies depending on the state of the cells, culture conditions, and the like, but is, for example, about 3 days to 3 weeks, and Days to 2 weeks, more preferably about 1 week.
- a cell layer corneal epithelium-like sheet in which cells derived from cells for corneal epithelium are layered on the amniotic membrane-derived medical material.
- the corneal epithelium-like sheet can be used as a transplant material (substitute for corneal epithelium) for a patient having a damaged or defective cornea, together with an amniotic membrane-derived medical material used as a culture substrate.
- the amniotic membrane-derived medical material layer is implanted into the corneal epithelial defect so that it is on the eyeball side.
- a part of the amniotic membrane-derived medical material or a part of the material completely removed can be used as a graft. Removal of the amniotic membrane-derived medical material can be performed by chemical treatment with EDTA or the like, enzymatic treatment with a proteolytic enzyme or the like, or physical treatment such as reptile with tweezers or the like.
- a corneal epithelium-like cell layer (corneal epithelium-like sheet) in which cells derived from cells for corneal epithelium are layered is prepared. It can be considered as providing a transplant material for corneal transplant (a sheet for corneal epithelial transplant) formed on a medical material.
- the method of the present invention can also be used as a method for producing a sheet for corneal epithelial transplant having such a structure.
- oral mucosal epithelial cells are used as the cells for corneal epithelium
- cells derived from oral mucosal epithelial cells are stratified in the corneal epithelium-like sheet.
- the fact that the cells are derived from oral mucosal epithelium can be confirmed using, as an index, the expression of keratin 4 or keratin 13 specific to oral mucosal epithelial cells in the cells constituting the corneal epithelium-like sheet.
- the expression of keratin 3 can be used as an index.
- This keratin 3 is known as one of the corneal-specific keratins, but has been confirmed to be expressed in oral mucosal epithelial cells.
- keratin 3 which is a corneal-specific keratin, it can be said that it is preferable to use oral mucosal epithelial cells as a material for preparing a composition for corneal epithelial transplantation.
- the corneal epithelium-like sheet provided in the present invention preferably has some of the following properties or characteristics. Particularly preferably, it has all the following properties or characteristics.
- the cells in the top layer are not keratinized. This is one of the characteristics of the corneal epithelium. By recognizing this feature, it is expected that the corneal epithelium-like sheet of the present invention will resemble the corneal epithelium and exhibit the same function as the corneal epithelium.
- keratinization is also called keratinization, and refers to a phenomenon in which keratin is generated in cells and organelles such as nuclei are lost. Whether or not the cells are keratinized can be confirmed, for example, by using flattened cells or the presence or absence of nuclei as an index.
- the cells in the uppermost layer are flat. That is, the cell layer derived from the cells for forming the cornea has a structure in which a layer made of flat cells is formed on a layer made of substantially columnar cells. It is thought that the airtightness between cells is enhanced by the flat cells covering the uppermost layer, and the lower barrier function is exhibited.
- the cells in the uppermost layer are flat.
- the corneal epithelium-like sheet of the present invention is similar to the corneal epithelium, and is expected to exhibit the same function as the corneal epithelium. (3) It has a barrier function.
- the barrier function refers to a function of preventing infiltration of a liquid or a gas from the surface layer and diffusion of the liquid through the surface layer.
- the cornea has a barrier function, so that it can retain moisture on its surface, thereby exhibiting the function of withstanding blinks. Therefore, providing a barrier function is one of the important characteristics required for a corneal implant.
- the corneal epithelium-like sheet of the present invention is similar to the corneal epithelium, and is expected to exhibit the same function as the corneal epithelium.
- Whether or not it has a barrier function can be determined by, for example, the degree of infiltration of a solution containing a labeling substance such as horseradish peroxidase (Horseradish peroxidase).
- a labeling substance such as horseradish peroxidase (Horseradish peroxidase).
- Another embodiment of the amniotic membrane-derived medical material of the present invention relates to a method for producing a corneal endothelium-like sheet.
- a corneal endothelium-like cell layer is constructed by culturing corneal endothelial cells on an amniotic membrane-derived medical material.
- a corneal endothelium-like sheet is prepared according to the following procedure.
- Corneal endothelial cells are harvested from the recipient or the cornea of a suitable donor in a conventional manner. For example, after the Descemet's membrane and endothelial cell layer of the corneal tissue are detached from the corneal stroma, they are transferred to a culture dish and treated with dispase. As a result, the corneal endothelial cells fall off from the Descemet's membrane. Corneal endothelial cells remaining in the Descemet's membrane can be shed by, for example, pipetting. After removing the Descemet's membrane, the corneal endothelial cells are cultured in an appropriate culture medium in which corneal endothelial cells can grow.
- DMEM Dullbecco's Modified Eagle's Medium
- FBS ⁇ fetal serum
- b-FGF basic-fibroblast growth factor
- EGF epidermal growth factor
- insul insulator-like growth factor
- type I collagen, type IV collagen, fipronect It is preferable to use one coated with tin, laminin, or extracellular matrix of porcine endothelial cells. This is because adhesion of the corneal endothelial cells to the surface of the culture vessel is promoted, and good proliferation is performed.
- the temperature conditions for culturing the corneal endothelial cells are not particularly limited as long as the corneal endothelial cells grow, for example, about 25 ° C. to about 45 ° C., and preferably about 30 ° C. It is about 40 ° C, more preferably about 37 ° C.
- the cultivation time varies depending on the condition of the cells used and the like, but is, for example, 7 to 14 days.
- the corneal endothelial cells of the recipient cannot be obtained or are difficult to obtain, corneal endothelial cells other than the recipient can be used. In this case, the donor should be selected in consideration of immunocompatibility. Is preferred.
- Subculture can be performed after the corneal endothelial cells subjected to the culture have proliferated. Preferably, subculture is performed at the time of subconfluent or confluent.
- Subculture can be performed as follows. First, the cells are peeled from the culture vessel surface by treating with trypsin-EDTA or the like, and then the cells are collected. A culture solution is added to the collected cells to make a cell suspension. When recovering the cells, or after the recovery, it is preferable to perform centrifugation. By such centrifugation, a cell suspension having a high cell density can be prepared. The conditions for the centrifugation here include, for example, 500 rpm (30 g) to 1000 rpm (70 g) for 1 to 10 minutes.
- the cell suspension is seeded in a culture vessel in the same manner as in the initial culture described above, and used for culture.
- the subculture can be performed under the same culture conditions as in the above initial culture. Culture time depends on the condition of cells used For example, it is 7 to 21 days.
- the above subculture can be performed multiple times as necessary. By repeating the subculture, the number of cells can be increased, and a cell suspension having a high cell density can be prepared. Finally, it is preferable to prepare a cell suspension having a cell density of about 5 ⁇ 10 5 cells / ml to 20 ⁇ 10 5 cells Zml.
- the cell suspension is seeded on amniotic membrane-derived medical material and used for culture.
- the number of cells to be seeded is adjusted so that a cell layer having a desired cell density is formed in the corneal endothelium-like sheet finally produced.
- the cell density of about 2, about 4, 000 to cell layer of cells / mm 2 is formed, for example, 1 mm 2 per about 3,000 to about 7, 500 000, preferably from about Seed 5,000 to 7,500 cells.
- the culture can be performed under the same conditions as in the above-mentioned initial culture and the like.
- the cultivation time varies depending on the condition of the cells to be used, but is, for example, 3 to 21 days.
- corneal endothelial cells are preferably seeded on the side of the exposed epithelium (ie, the basement membrane side). This is because this surface side contains a large amount of type IV collagen, and it is considered that the seeded corneal endothelial cells can be favorably adhered and proliferated.
- Culture of corneal endothelial cells on amniotic membrane-derived medical material can be performed, for example, as follows. First, a container having a bottom surface made of a pore-sized membrane through which a culture solution can pass (hereinafter, also referred to as “culture insert”) is placed in a culture container with the bottom surface down.
- the amnion-derived medical material is placed on the bottom of the culture insert (inside the culture insert). Then, the cell suspension is seeded and cultured on the amniotic membrane-derived medical material.
- the amniotic membrane-derived medical material can be previously placed on the bottom surface of the culture insert. That is, for example, an amniotic membrane-derived medical material is placed on the bottom of the culture insert (a drying process may be performed after the placement), the culture insert is placed in a culture vessel, and then a cell suspension is seeded and cultured. May be.
- a film that can be used on the bottom of the culture insert include a film made of polycarbonate and having a pore size of about 0.4 ⁇ to 3.0 ⁇ .
- a polyester film or the like can be used. Such membranes are commercially available and can be easily obtained. Centrifugation can be performed after seeding the cell suspension in the culture insert. Thereby, the cell density on the amniotic membrane-derived medical material can be increased. In addition, adhesion of cells to the surface of the amniotic membrane-derived medical material is also improved. Further, the effect of reducing the bias of the cell density can be obtained.
- the conditions of the centrifugation here include, for example, 500 rpm (30 g) to OOO rpm (70 g) for 1 to 10 minutes.
- a corneal endothelium-like sheet in which a cell layer composed of cells derived from corneal endothelium is formed on the amniotic membrane-derived medical material.
- This corneal endothelium-like sheet can be used as a transplant material (replacement of corneal endothelium) in the treatment of diseases requiring corneal endoderm transplantation, for example, bullous keratopathy, corneal edema, corneal vitiligo, and keratoconus.
- a part or all of the amniotic membrane-derived medical material ie, only the cell layer
- the removal of the amniotic membrane-derived medical material can be performed by chemical treatment with EDTA or the like, enzymatic treatment with proteolytic enzymes or the like, or physical treatment such as tweezers or the like.
- Culture of corneal endothelial cells (including initial culture, subculture, and culture on medical material derived from amniotic membrane) can be performed in the presence of supporting cells. By culturing in the presence of supporting cells, effects such as improvement of the proliferation efficiency of corneal endothelial cells and promotion of differentiation can be expected.
- Supporting cells include, for example, 10T1 / 2 fibrous cells (Hy I dah IL: Primary eel I Cutures from human embryonic corneas.J Cel I Sc i. 66: 343-351, 1984.), 3T3 cells (Swiss mouse 3T3 Cells, mouse NIH3T3 cells, 3T3J2 cells, etc.), and keratocytes.
- the distance between corneal endothelial cells and supporting cells it is preferable to interpose a pore-sized separator through which the supporting cells cannot pass.
- This separator can prevent feeder cells from entering the corneal endothelial cells during culture.
- supporting cells there is no possibility that supporting cells are mixed in the finally obtained corneal endothelium-like sheet. This means that it becomes possible to prepare a corneal endothelium-like sheet without the problem of immune rejection by supporting cells, which is extremely clinically significant.
- mitomycin C or the like it is preferable to inactivate the feeder cell using mitomycin C or the like in advance.
- the separator the same separator as that used for the culture insert can be used. That is, for example, a membrane made of polycarbonate, polyester, or the like and having a pore size of about 0.4 m to 3.0 im can be used.
- Cell density of feeder cells for example, about 1 X 10 2 or cm 2 or more, preferably about 1 X 10 2 pieces / cm 2 ⁇ about 1 X 10 7 cells / cm 2, more preferably about 1 X 10 3 cells / cm 2 to about 1 ⁇ 10 5 pieces / cm 2 .
- Examples of a method for transplanting the corneal endothelium-like sheet prepared by the above method include a full-thickness trepanion method and a deep corneal ablation method.
- the former method is to collect the entire corneal layer once using trepan, replace the corneal endothelial cell layer with the corneal endothelium-like sheet of the present invention, and then return to the recipient. Can be performed as follows.
- the recipient Cut the cornea of the (host) in all layers with trepan, and collect a part (or all) of the cornea in a button-like shape.
- the Descemet's membrane and the corneal endothelial cell layer are separated from the collected corneal pieces.
- the corneal endothelium-like sheet of the present invention is adhered to the exposed cornea substantially.
- the amniotic membrane-derived medical material is placed on the substantial side.
- the corneal piece is then returned to the recipient and fixed with sutures.
- the deep corneal ablation method does not remove the entire corneal layer but only the deep corneal layer, and is considered to be a method that places less burden on the recipient. Specifically, it can be performed as follows. First, a part of the corneal stroma of the recipe is exfoliated, and a part of the deep corneal stratum and a part of the Descemet's membrane and the endothelial cell layer are cut off. In addition, only the endothelial cell layer or the endothelial cell layer and the Descemet's membrane may be peeled off. Only the damaged recipient corneal endothelial cells may be removed. Next, the corneal endothelium-like sheet of the present invention is inserted into the resection using a spatula or the like. Inject air into the anterior chamber as needed to fix the graft.
- Whether or not the transplanted corneal endothelium-like sheet exerts a barrier function and a pump function in the same manner as the corneal endothelial cell layer in a living body can be determined, for example, by examining changes in corneal thickness after transplantation and occurrence of edema. You can check.
- the process of amniotic membrane treatment was performed in the order of (1) washing, (2) trimming, and (3) storage. All processes It is desirable that the operation be performed in a clean draft, and that all containers and utensils used be sterile and petri dishes should be sterile, disposable (disposable) types. used.
- the blood component adhering to the collected amniotic membrane was removed while washing with physiological saline, and further washed with a sufficient amount of physiological saline (0.005% of loxacin added). Subsequently, the cells were washed three times with a phosphate buffer (PBS) supplemented with penicillin streptomycin (50 IU). Next, the amniotic membrane was transferred into a petri dish, and divided into about 4 ⁇ 3 cm pieces using scissors. After the division, the amniotic membrane in good condition was selected based on the shape and thickness.
- PBS phosphate buffer
- penicillin streptomycin 50 IU
- the preservation solution was placed in a 2 cc sterile cryotube each with Ice, collected, washed, and sorted for each amniotic membrane, labeled and stored in a deep freezer at 180 ° C.
- Glycerol in DMEM Dulbecco's Modified Eagle Medium: GIBC0BRL
- the stored amniotic membrane was to be used for a period of three months, after which time it was incinerated.
- the following epithelial treatment may be performed without performing such storage processing.
- amniotic membrane stored at 80 ° C was thawed at room temperature, it was washed twice with a phosphate buffer (PBS) supplemented with penicillin-streptomycin (50 peaks).
- PBS phosphate buffer
- Amniotic membrane 0.02% EDTA solution washed soaked in (Nacalai tesque Inc.) (100 ⁇ dish) at 37 in a C0 2 incubator, and allowed to react for 1 hour. After the reaction, the amniotic membrane was washed twice with a sufficient amount of PBS, and the epithelium was manually removed (removed) using a cell scraper (Nunc USA) under a stereoscopic microscope.
- FIG. 1 is an amniotic membrane image by a scanning electron microscope.
- A is an image of the amniotic membrane surface in a normal state (without epithelial treatment)
- B is an image of the amniotic membrane surface after the epithelial treatment (0.02% EDTA solution).
- the amniotic membrane was sandwiched between a pair of sterilized plastic frames, and fixed with clips. Transfer the entire frame into a deep freezer at 80 ° C and confirm that the amniotic membrane has been frozen. Went. According to the instructions for use, conditions were set so that a sufficient dried product could be obtained.
- the lyophilized amniotic membrane was removed from the frame, transferred to a double-layered bag made of polyamide nylon on the outside, and polyethylene on the inside, and vacuum-packed using a household vacuum pack (frame nova, magic pack). The vacuum-packed amniotic membrane thus obtained was irradiated with T-rays (about 25 kGy) and sterilized.
- amniotic membrane after the sterilization treatment was stored in a vacuum packed state at room temperature until immediately before use.
- the state immediately after freeze-drying was maintained 12 months after the start of storage.
- Subsequent experiments were performed using dried amniotic membranes stored at room temperature for one month.
- the dried amniotic membrane obtained in Example 5 immunoassay was performed by the following procedure to confirm that the basement membrane remained on the surface from which the epithelium had been removed and that the structure was retained.
- the dried amniotic membrane was immersed in PBS for reconstitution, excised to an appropriate size, and frozen and embedded in an OCT compound. Thereafter, slide sections were prepared by slicing with a cliche. After washing the slide sections with PBS, they were packed with 1% fetal calf serum (FBS) to suppress nonspecific antibody reactions. Thereafter, the primary antibody was reacted at room temperature for 1 hour.
- FBS fetal calf serum
- the primary antibody an antibody (manufactured by Cosmo Bio Inc.) that specifically binds to collagens l-V and VI, and fibronectin and laminin 5, respectively was used. After the reaction was completed, the cells were washed with PBS containing triton-X for 15 minutes under the conditions of three times, and then reacted with a fluorescently labeled antibody (secondary antibody) at room temperature for 1 hour. Next, the cells were washed with PBS for 3 times for 15 minutes, sealed, and then observed with a confocal microscope.
- an antibody manufactured by Cosmo Bio Inc.
- the basement membrane can be maintained even after lyophilization after the epithelium is removed, and the remaining basement membrane is hardly damaged even after sterilization.
- the sample in which the epithelium was removed by dispase AMD (-) d i spase)
- these characteristic components of the basement membrane were not detected. This is considered to indicate that the basement membrane was destroyed by the dispase treatment.
- Corneas collected from 6-week-old Japanese white rabbits were immersed in DMEM containing 10% fetal calf serum (FBS) to remove unnecessary tissues such as conjunctiva and corneal endothelium. Thereafter, the tissue was washed with a phosphate buffer (PBS) and immersed in a phosphate buffer (PBS) containing 1.2 U / ml dispase (Naca lai tesque) at 37 ° C for 1 hour. The treated tissue was taken out and immersed in 0.02% EDTA at room temperature for 2 minutes and then in a phosphate buffer at room temperature for 2 minutes to stop the dispase activity. Cornea in DMEM containing 10% fetal serum (FBS) After the epithelial cells were removed, the corneal epithelial cells were concentrated and collected by centrifugation.
- FBS fetal calf serum
- 3T3 cells MIH-3T3 cells
- 3T3 cells MIH-3T3 cells
- 3T3 cells which had been cultured in advance and became confluent in a 75F flask (BD Falcon), were immersed in a 0.05% mitomycin C solution for 2 hours to suppress the growth activity of 3T3. Subsequently, mitomycin C was removed by washing several times with a phosphate buffer solution (PBS). After treating the cells with 0.05% trypsin-EDTA solution, pipetting was performed to obtain a cell suspension (3T3 cell suspension).
- PBS phosphate buffer solution
- the freeze-dried amniotic membrane obtained in Example 5 was immersed in PBS at room temperature until it was sufficiently restored.
- a 3 cell suspension was seeded on a culture dish so as to have a cell density of about 1 ⁇ 10 4 cells / cm 2 , and cultured at 37 ° C. and 5% CO 2 .
- FIG. 3 is a schematic sectional view showing a state during the culture. Culture insulator 2 is left still in culture dish 1, and 3 cells 3 cell layer 5 are formed on the bottom of culture dish 1.
- Reference numeral 6 is a medium.
- the medium was DMEM / Ham F12 mixed medium (mixed volume ratio 1: 1), 10% FBS, insulin (5 mg / inl), cholera toxin (0.1 nM), penicillin streptomycin (50 IU / ml), human A solution supplemented with recombinant epidermal growth factor (EGF) (10 ng / ml) was used.
- the cells were cultured in the above medium for 3 weeks (Submerge).
- the air-lifting method is a method in which the liquid surface of the medium is brought up to the surface of a cell layer derived from corneal epithelial cells formed on the amniotic membrane, and the surface of the cell layer is exposed to the air. During Submerge, the medium was changed every other day, and after a-1 ifting, the medium was changed every day for culturing.
- a cell layer similar to corneal epithelium was formed about 20 days after the start of culturing (Fig. 4).
- the cells are stratified in 4 to 5 layers as in the case of normal corneal epithelium.
- the amniotic membrane side of this cell layer there was a relatively column-shaped cell group similar to basal cells.
- the cells on the outermost layer had a flat type but had nuclei, and the surface thereof, which was different from the skin, was not keratinized.
- a cell layer corneal epithelium-like layer
- transplantation sheet having the corneal epithelium-like cell layer formed on the amniotic membrane (hereinafter also referred to as “corneal epithelium transplantation sheet”) prepared by the above method.
- the corneal and conjunctival epithelium were removed from the outer side of the limbus with a thickness of 100 im using a knives knife. Since this operation eliminates epithelial cells including corneal epidermal stem cells, it can be considered that artificial eye surface stem cell exhaustion is reproduced.
- the surface of the eye before transplantation was observed (Fig. 5, upper column).
- the sheet for corneal epithelial transplantation was transplanted to a region slightly inside the limbus. At the time of transplantation, it was sutured to surrounding tissue using 10-0 nylon thread. After implantation, a therapeutic soft contact lens was sewn over the implant.
- the ocular surface had the same transparency as the corneal epithelium transplantation sheet before transplantation.
- the ocular surface subjected to the transplantation was observed 2 days after the transplantation and 7 days after the transplantation, respectively.
- the transplanted corneal epithelial transplantation sheet maintained its transparency, and it was confirmed by full-year Resin staining that it remained on the ocular surface without any defects (Fig. 5 lower column).
- the graft did not show full-year resin staining, and thus it was confirmed that the sheet for corneal epithelial transplantation had the same barrier function as corneal epithelium.
- the full-year-old Resin staining showed that the full-year-old Resin stained all around the graft, indicating that the tissue existing in the transplanted area was not a contamination of the remaining conjunctival epithelium around it. It was confirmed that. Since the cells of the corneal epithelium are bonded to each other by a fine adhesive structure, the dye of full-year-old rescein does not infiltrate from the surface thereof, and does not show staining property in fluorescein staining. On the other hand, when the adhesion between cells is loosened or the cells themselves detach and the barrier function is impaired, infiltration of a full-year-old resin dye occurs and stains the tissue.
- the transplanted corneal epithelial transplantation sheet has a barrier function similar to that of the corneal epithelium can be confirmed by examining the staining properties of full-age resin staining. Seven days after the transplantation, it was confirmed that the transplanted corneal epithelial transplant sheet remained on the ocular surface, and extended to the surroundings compared to the state two days after the transplantation. The entire ocular surface was covered (Fig. 6, lower column). In addition, as shown in FIG. 6, the graft did not show fluorescein staining, and it was confirmed that the graft maintained the barrier function required for corneal epithelium. Transparency was also maintained (upper column in Figure 6).
- the corneal epithelial transplant sheet made from dried amniotic membrane was engrafted and extended on the ocular surface.
- the corneal epithelium transplant sheet produced by the above-described method contains a cell layer that functions well as a substitute for the corneal epithelium, and is a transplant material for reconstructing the ocular surface in the case of corneal damage or defect. It was confirmed that it could be suitably used as.
- the oral mucosal epithelium attached to the tooth extraction of the donor is separated from the enamel cement transition.
- the collected oral mucosal epithelium is immersed twice in phosphate buffered saline (PBS) containing 50 U / ml penicillin streptomycin and genosine for 30 minutes at room temperature. Then, the tissue was immersed in a phosphate buffer (PBS) containing 1,2 U dispase (Nacalai tesque) for 1 hour at 37 ° C, followed by a 0.05% tribcine-EDTA solution (GIBC0BRL). Separate the cells by infiltration for 30 minutes using the following procedure. Enzyme activity is stopped by immersion in MEM containing 10% fetal calf serum (FBS).
- PBS phosphate buffered saline
- FBS fetal calf serum
- oral mucosal epithelial cells oral mucosal epithelial cells
- oral mucosal epithelial cells are isolated by removing extra tissues with a 60-m cell fMter.
- a corneal epithelium-like cell layer is formed in the same manner as described in Examples 8 and 9.
- DMEM (GIBC0BRL), 10% FBS (Dainippon Pharmaceutical Co., Ltd.), b-FGF (Invitrogen II) 2 ng / ml, penicillin (50 U / tnl) —streptomycin (50 ig / ml) (Nacalai) Can be used. After removing the supernatant, add the culture solution to make a cell suspension of about 1 ml. The cell suspension was seeded in 24-well culture dish was type IV collagen-coated, 37, cultured under the condition of 53 ⁇ 4 C0 2. Thereafter, the culture medium is replaced every 48 hours.
- the culture solution is removed, and the cells are washed three times with 1 ml of calcium-free PBS. After washing, to stand for 3 minutes at 0,05% Bok Ribushin (trypsin)-EDTA and 200 I was added 37 ° C, of 5% C0 2 conditions. This treatment causes the adherent endothelial cells to float on the bottom of the culture dish. After adding 5 ml of the culture solution, transfer the cell suspension to a 15 cc centrifuge tube, and centrifuge at 1,000 rpm, 70 g for 3 minutes. After removing the supernatant, add the culture to make about 2 ml of cell suspension.
- Bok Ribushin trypsin
- the cell suspension was seeded in 35 strokes culture dishes were Bok type IV collagen code, cultured in a 37 ° C, 5% C0 2 conditions. Thereafter, change the culture medium every 48 hours. When the cells become confluent, perform the same operation as above, and repeat the subculture until the required number of cells is obtained.
- a corneal endothelial cell layer is prepared by the following procedure using the obtained cell suspension.
- a 6-well culture dish (Corning, NY) and a culture insert (culture insert: culture insert, polycarbonate, average pore size 3.0 ⁇ Corning, NY) are used as culture equipment. be able to. The same culture medium as used above can be used. First, place the culture insert in the culture dish.
- Example 5 the dried amniotic membrane obtained in Example 5 reconstituted with PBS is spread and laid on the culture insert (on the bottom surface) with the side where the epithelium was present facing up. Subsequently, the cell suspension is seeded on the amniotic membrane so as to be about 5,000 cells / band 2 . Then, centrifuge the culture dish at 1,000 rpm, 70 g for 3 minutes. After centrifugation, cultured in a 37 ° C, 5% C0 2 conditions. As a result, a single cell layer is constructed.
- the present invention is not limited to the description of the embodiment and the example of the above invention. Various modifications are included in the present invention without departing from the scope of the claims and within the scope of those skilled in the art. Possibility of industrial use
- an amniotic membrane-derived medical material which is easy to handle and can sufficiently withstand the required sterilization treatment.
- the immunogenicity is extremely low, and at least a part of the basement membrane remains in a state where the original structure is maintained at a high level.
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US10/546,774 US20060153928A1 (en) | 2003-02-26 | 2004-02-17 | Aminion-origin medical material and method of preparing the same |
EP04711695A EP1604695A1 (en) | 2003-02-26 | 2004-02-17 | Amnion-origin medical material and method of preparing the same |
JP2005502999A JPWO2004078225A1 (ja) | 2003-02-26 | 2004-02-17 | 羊膜由来医用材料、及びその作製方法 |
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WO2004078225A1 true WO2004078225A1 (ja) | 2004-09-16 |
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PCT/JP2004/001692 WO2004078225A1 (ja) | 2003-02-26 | 2004-02-17 | 羊膜由来医用材料、及びその作製方法 |
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US (1) | US20060153928A1 (ja) |
EP (1) | EP1604695A1 (ja) |
JP (1) | JPWO2004078225A1 (ja) |
KR (1) | KR20050105242A (ja) |
CN (1) | CN1753697A (ja) |
CA (1) | CA2516316A1 (ja) |
WO (1) | WO2004078225A1 (ja) |
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2004
- 2004-02-17 JP JP2005502999A patent/JPWO2004078225A1/ja active Pending
- 2004-02-17 CN CNA2004800048560A patent/CN1753697A/zh active Pending
- 2004-02-17 EP EP04711695A patent/EP1604695A1/en not_active Withdrawn
- 2004-02-17 CA CA002516316A patent/CA2516316A1/en not_active Abandoned
- 2004-02-17 US US10/546,774 patent/US20060153928A1/en not_active Abandoned
- 2004-02-17 KR KR1020057015708A patent/KR20050105242A/ko not_active Application Discontinuation
- 2004-02-17 WO PCT/JP2004/001692 patent/WO2004078225A1/ja active Application Filing
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WO2006075602A1 (ja) * | 2005-01-14 | 2006-07-20 | Arblast Co., Ltd. | 羊膜を用いたシート状組成物及びその作製方法 |
WO2006129673A1 (ja) * | 2005-05-30 | 2006-12-07 | Arblast Co., Ltd. | 組織再建用材料及びその使用 |
WO2007013331A1 (ja) * | 2005-07-25 | 2007-02-01 | Arblast Co., Ltd. | シート状組成物 |
KR101313033B1 (ko) | 2005-07-25 | 2013-10-01 | 시게루 기노시타 | 시트형 조성물 |
JP5109030B2 (ja) * | 2005-07-25 | 2012-12-26 | 茂 木下 | シート状組成物 |
US8231908B2 (en) | 2005-07-25 | 2012-07-31 | Arblast Co., Ltd. | Sheet-like composition |
EP1932549A1 (en) * | 2005-08-26 | 2008-06-18 | National University Corporation Toyama University | Dried amnion and method for drying treatment of amnion |
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EP1932549A4 (en) * | 2005-08-26 | 2009-11-04 | Univ Toyama Nat Univ Corp | DRIED AMNION AND METHOD FOR DRYING AMNION |
JP2007054015A (ja) * | 2005-08-26 | 2007-03-08 | Toyama Univ | 乾燥羊膜及び羊膜の乾燥処理方法 |
WO2007023750A1 (ja) | 2005-08-26 | 2007-03-01 | National University Corporation University Of Toyama | 乾燥羊膜及び羊膜の乾燥処理方法 |
JP5255846B2 (ja) * | 2006-01-19 | 2013-08-07 | 千寿製薬株式会社 | 生体内で細胞増殖可能な角膜内皮製剤 |
JP2008036345A (ja) * | 2006-08-10 | 2008-02-21 | Toyama Univ | 乾燥羊膜からなる眼表面の再建用医療材料 |
JP2008199972A (ja) * | 2007-02-21 | 2008-09-04 | Toyama Univ | 培養重層上皮シートの作製方法 |
WO2008131639A1 (fr) * | 2007-04-25 | 2008-11-06 | Zhongshan Ophthalmic Center, Sun Yat-Sen University | Substrat de cornée produit par suppression de cellules et procédé de préparation de celui-ci |
WO2010084970A1 (ja) | 2009-01-23 | 2010-07-29 | 国立大学法人大阪大学 | 標的細胞誘導用フィーダー細胞 |
WO2011074208A1 (ja) * | 2009-12-18 | 2011-06-23 | 国立大学法人東北大学 | 皮膚真皮又は羊膜透明化による角膜移植材料調製法 |
US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
US11305035B2 (en) | 2010-05-14 | 2022-04-19 | Musculoskeletal Transplant Foundatiaon | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
US10335439B2 (en) | 2013-03-11 | 2019-07-02 | Jcr Pharmaceuticals Co., Ltd. | Method for producing human corneal epithelium sheet |
US10531957B2 (en) | 2015-05-21 | 2020-01-14 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
US11596517B2 (en) | 2015-05-21 | 2023-03-07 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
CN108498865A (zh) * | 2018-04-11 | 2018-09-07 | 济南磐升生物技术有限公司 | 一种人造角膜的制备方法及应用 |
CN108498865B (zh) * | 2018-04-11 | 2020-11-06 | 济南磐升生物技术有限公司 | 一种人造角膜的制备方法及应用 |
Also Published As
Publication number | Publication date |
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CN1753697A (zh) | 2006-03-29 |
US20060153928A1 (en) | 2006-07-13 |
EP1604695A1 (en) | 2005-12-14 |
KR20050105242A (ko) | 2005-11-03 |
CA2516316A1 (en) | 2004-09-16 |
JPWO2004078225A1 (ja) | 2006-06-08 |
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