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  • C07C69/00—Esters of carboxylic acids; Esters of carbonic or haloformic acids
  • C07C69/013—Esters of alcohols having the esterified hydroxy group bound to a carbon atom of a ring other than a six-membered aromatic ring
• • A—HUMAN NECESSITIES
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  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/24—Antidepressants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
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  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
  • C07C45/61—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups
  • C07C45/62—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by hydrogenation of carbon-to-carbon double or triple bonds
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
  • C07C45/61—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups
  • C07C45/64—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds by reactions not involving the formation of >C = O groups by introduction of functional groups containing oxygen only in singly bound form
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C45/00—Preparation of compounds having >C = O groups bound only to carbon or hydrogen atoms; Preparation of chelates of such compounds
  • C07C45/78—Separation; Purification; Stabilisation; Use of additives
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
  • C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
  • C07C49/703—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups
  • C07C49/713—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups a keto group being part of a six-membered ring
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
  • C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
  • C07C49/703—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups
  • C07C49/723—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups polycyclic
  • C07C49/727—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups polycyclic a keto group being part of a condensed ring system
  • C07C49/733—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups polycyclic a keto group being part of a condensed ring system having two rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C49/00—Ketones; Ketenes; Dimeric ketenes; Ketonic chelates
  • C07C49/587—Unsaturated compounds containing a keto groups being part of a ring
  • C07C49/703—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups
  • C07C49/743—Unsaturated compounds containing a keto groups being part of a ring containing hydroxy groups having unsaturation outside the rings, e.g. humulones, lupulones
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
  • C07C2602/00—Systems containing two condensed rings
  • C07C2602/36—Systems containing two condensed rings the rings having more than two atoms in common
  • C07C2602/46—Systems containing two condensed rings the rings having more than two atoms in common the bicyclo ring system containing nine carbon atoms

Definitions

• the present invention relates to hyperforin and adhyperforin derivatives and the use thereof in the pharmaceutical and/or nutritional field, in particular in the treatment of depression and Alzheimer's disease.
• Hyperforin a floroglucin derivative
• Hyperforin is one of the main components of the lipophilic fraction of Hypericum perforatum flowering tops; said fraction also contains adhyperforin, a hyperforin higher homologue, although in lower concentration (Erdelmeier C.A.J., Pharmacopsychiatry, 31, 2-6, 1998).
• Hyperforin has recently been the object of numerous studies that establish its important role as an antidepressant (Pharmacopsychiatry, 31 Suppl.l, 1-60. 1998). Furthermore, it is recognized that the extracts of Hypericum perforatum can be used for the prophylaxis and treatment of neurodegenerative diseases, inter alia Alzheimer's disease (WO/9940905, WO0057707). In particular, hyperforin and adhyperforin salts with inorganic cations or ammonium salts were described for this purpose (WO9941220).
• hyperforin and adhyperforin derivatives obtainable by reduction of all double bonds of the isoprene chains and/or by reduction of the keto groups at the 1- and 10- positions to hydroxy groups not only have high stability, but also possess anti depress ant, anxiolytic and anti- neurodegenerative activities surprisingly higher than hyperforin and adhyperforin.
• Object of the present invention is therefore the use of hyperforin and adhyperforin derivatives of formula (I)
• R is methyl or ethyl
• R 2 is hydrogen, a pharmaceutically acceptable inorganic or organic base cation or a straight or branched C 2 -Cs acyl residue
• R t is 3-methyl-but-l-yl and oxo groups are present at the 1- and 10- positions
• R ⁇ is 3-methyl-2-buten-l-yl and hydroxy groups are present at the 1- and 10- positions
• Ri is 3-methyl-but-l-yl and hydroxy groups are present at the 1- and 10- positions.
• Preferred compounds of formula (I) as defined at a) are those in which R 2 is hydrogen, in the following defined octahydrohyperforin (la) and octahydroadhyperforin (lb):
• Preferred compounds of formula (I) as defined at c) are those in which R2 is hydrogen (in the following defined dodecahydrohyperform le and dodecahydroadhyperforin If), dodecahydrohyperform (le):
• R 2 is lithium (octahydrohyperforin lithium salt Ig and octahydroadhyperforin lithium salt Ih), octahydrohyperforin lithium salt (Ig) tetrahydrohyperforin (Ic) being most preferred:
• R 2 is acetyl (acetyloctahydrohyperforin li and acetyloctahydroadhyperforin II), acetyloctahydrohyperforin (li) tetrahydrohyperforin (Ic) being most preferred:
• Dodecahydrohyperform (le), dodecahydroadhyperforin (If), acetyloctahydrohyperforin (li) and acetyloctahydroadhyperforin (II) are novel compounds and are also part of the present invention.
• the compounds of formula (la) and (lb) are obtained through reduction of the isoprene side chains by catalytic hydrogenation, using for example palladium on charcoal or Nickel/Raney.
• the compounds of formula (Ic) and (Id) are obtained by reduction of the keto groups at the 1- and 10- positions with hydrides, selected for example from NaBH 4 , Redal ® , Vitride ® , LiAlH 4 .
• the compounds of formula (le) and (If) are obtained by reducing first the isoprene side chains and then the keto groups at the 1- and 10- positions according to what described above.
• the flowering tops of Hypericum perforatum can be extracted with alcohols or aliphatic ketones, either pure or in a mixture thereof with water or with gas in supercritical conditions; the resulting extract is partitioned between n-hexane and aqueous solutions of aliphatic alcohols.
• the hexane solution is extracted with alkaline methanol to extract hyperforin and adhyperforin.
• the methanolic solution is acidified, then treated with a weakly basic ion exchange resin, which selectively retains hyperforin and adhyperforin.
• the resin is eluted with acidic methanol and the eluate is concentrated to small volume, then diluted with water and back-extracted with n-hexane.
• the hexane solution is concentrated to small volume and the resulting concentrate is ready for derivatization.
• the residue is taken up in chlorinated solvents and the suitable reactive is added thereto, according to the procedures reported in the examples.
• the compounds of the invention have shown antidepressant effect, which was evaluated in the rat by the forced swimming test, evaluating the parameters: straggling, floating and swimming according to what described by Cervo et al. in Neuropharmacology, 26, 14969-72, 1987.
• the compounds were administered in 3 doses: 30 minutes after the pre-test, 5 hours and 30 minutes before the test.
• the results reported in the table below prove that the compounds of the invention are more active than parent hyperforin.
• the compounds of the invention also proved particularly active against Alzheimer's disease, due to their ability to increase APPs, the soluble, harmless form of Alzheimer Precursor Protein (APP). It is in fact known that proteolytic cleavage of Alzheimer Precursor Protein (APP) is mediated both by ⁇ - and ⁇ -secretase, inducing an increased production of amyloid peptide A -42 (which also plays a central role in the appearance of Alzheimer's disease), and ⁇ -secretase, giving raise to soluble APPs which have no pathogenic activity (Eslr W.P., Wolfe M.S., Science, 293,1449-54, 2001).
• APP Alzheimer Precursor Protein
• the compounds of the invention can be formulated according to conventional techniques, for example according to what described in Remington's Pharmaceutical Sciences Handbook, XNII Ed. Mack Pub., ⁇ .Y., U.S. A, in the form of soft-gelatin capsules, hard-gelatin capsules, tablets, suppositories; preferably the extract of the invention is formulated in soft- gelatin capsules or in controlled-release formulations.
• the dosage ranges from 10 to 100 mg per unit dose in the usual formulations and up to 200 mg in the controlled-release formulations, in this case the suggested dose being 200 mg per dose/daily.
• the compounds can be administered through the controlled-release transdermal route applying the formulation in the proximal area to the cerebral carotid artery derivations.
• the dosages of compound in these formulations range from 10 to 100 mg per dose/daily.
• This solution is neutralized and filtered through a weakly basic Amberlite resin, which selectively retains hyperforin and adhyperforin; the retained product is eluted again with methanol acidified with phosphoric acid; the methanolic eluate is concentrated under vacuum at 25°C, the diluted water and back-extracted with n-hexane until exhaustion of of hyperforin.
• the combined organic layers are decolourized with 0.3% charcoal, then dried over Na 2 S0 and concentrated to an oil below 40°C under vacuum. After solidification the oil yelds a wax (0.52 kg) containing approx. 90% of hyperforin.
• Example 2 Preparation of octahydrohyperforin dicyclohexylammonium salt 50 g of hyperforin obtained as described in Example 1 are dissolved in
• the resulting mixture is purified by column chromatography, using a 200 ml column packed with 100 ml of silica gel and petroleum ether/EtOAc 95 :5 as eluent mixture. Eluate fractions of approx. 20 ml are collected and the content is checked by TLC (petroleum ether/EtOAc 9:1). The more abundant product (1.5 g), crystallized from methanol has the following spectroscopical properties:
• reaction product is purified by column chromatography on 30 g of silica gel, eluting with a petroleum ether/ethyl acetate 9:1 mixture. Crystallization from methanol affords 150 mg of the desired compound having the following spectroscopical characteristics: EIMS m/z 586 [M] + .

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