WO2003035895A3 - Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon - Google Patents

Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon Download PDF

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Publication number
WO2003035895A3
WO2003035895A3 PCT/US2002/034570 US0234570W WO03035895A3 WO 2003035895 A3 WO2003035895 A3 WO 2003035895A3 US 0234570 W US0234570 W US 0234570W WO 03035895 A3 WO03035895 A3 WO 03035895A3
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WO
WIPO (PCT)
Prior art keywords
cells
rare
detection
assay
blood
Prior art date
Application number
PCT/US2002/034570
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English (en)
Other versions
WO2003035895A2 (fr
Inventor
Shawn Mark O'hara
Daniel Zweitzig
Brad Foulk
Original Assignee
Immunivest Corp
Shawn Mark O'hara
Daniel Zweitzig
Brad Foulk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immunivest Corp, Shawn Mark O'hara, Daniel Zweitzig, Brad Foulk filed Critical Immunivest Corp
Priority to KR10-2004-7006181A priority Critical patent/KR20040047971A/ko
Priority to IL16135702A priority patent/IL161357A0/xx
Priority to EP02795565A priority patent/EP1438419A2/fr
Priority to BRPI0213520-5A priority patent/BR0213520A/pt
Priority to JP2003538395A priority patent/JP2005507997A/ja
Priority to CA002465288A priority patent/CA2465288A1/fr
Publication of WO2003035895A2 publication Critical patent/WO2003035895A2/fr
Publication of WO2003035895A3 publication Critical patent/WO2003035895A3/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • G01N33/57488—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving compounds identifable in body fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis

Abstract

L'invention concerne un dosage biologique hautement sensible, qui utilise un procédé pour l'amplification, à amorçage spécifique de gènes, de bibliothèques d'ARNm provenant de cellules rares et de transcrits rares trouvés dans le sang. Le dosage biologique selon l'invention permet de détecter de l'ARNm rare (10 copies/cellule) trouvé dans 1 à 10 cellules isolées par enrichissement immunomagnétique. Le dosage biologique selon l'invention constitue une amélioration par rapport aux PCR multiplex et permet la détection efficace de séquences de codage rares pour des cellules carcinomateuses circulantes dans le sang. L'invention concerne également des procédés permettant le profilage de cellules isolées dans des tissus ou des fluides corporels et pouvant servir de compléments au diagnostic clinique de divers carcinomes, incluant la détection précoce et la classification des cellules tumorales circulantes. La surveillance de profils d'acides nucléiques et de protéines de cellules, dans des formats conventionnels ou bien dans des jeux ordonnés de micro-échantillons, facilite la gestion de l'intervention thérapeutique, incluant la mise en place de la thérapie, la surveillance de la réponse à la thérapie, la confirmation de la rémission et la détection d'une régression.
PCT/US2002/034570 2001-10-26 2002-10-28 Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon WO2003035895A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR10-2004-7006181A KR20040047971A (ko) 2001-10-26 2002-10-28 동일 샘플상에서의 광범위 핵산 및 이의 형태학적 특질에대한 다중 매개변수 분석법
IL16135702A IL161357A0 (en) 2001-10-26 2002-10-28 Multiparameter analysis of comprehensive nucleic acids and morphological features on the same sample
EP02795565A EP1438419A2 (fr) 2001-10-26 2002-10-28 Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon
BRPI0213520-5A BR0213520A (pt) 2001-10-26 2002-10-28 método para diagnosticar a gravidade de uma doença em um indivìduo de teste, e, kit de teste para triagem de uma amostra de paciente quanto à presença de células cancerosas circulantes
JP2003538395A JP2005507997A (ja) 2001-10-26 2002-10-28 同一試料についての包括的核酸および形態学的特徴のマルチパラメーター分析
CA002465288A CA2465288A1 (fr) 2001-10-26 2002-10-28 Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US33066901P 2001-10-26 2001-10-26
US60/330,669 2001-10-26
US36994502P 2002-04-04 2002-04-04
US60/369,945 2002-04-04

Publications (2)

Publication Number Publication Date
WO2003035895A2 WO2003035895A2 (fr) 2003-05-01
WO2003035895A3 true WO2003035895A3 (fr) 2004-01-08

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2002/034397 WO2003035894A2 (fr) 2001-10-26 2002-10-28 Analyse de parametres multiples d'acides nucleiques et de caracteristiques morphologiques d'ensemble sur le meme echantillon
PCT/US2002/034570 WO2003035895A2 (fr) 2001-10-26 2002-10-28 Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2002/034397 WO2003035894A2 (fr) 2001-10-26 2002-10-28 Analyse de parametres multiples d'acides nucleiques et de caracteristiques morphologiques d'ensemble sur le meme echantillon

Country Status (9)

Country Link
EP (2) EP1438398A2 (fr)
JP (1) JP2005507997A (fr)
KR (1) KR20040047971A (fr)
CN (1) CN1610752A (fr)
AU (1) AU2002360305A1 (fr)
BR (1) BR0213520A (fr)
CA (2) CA2465274A1 (fr)
IL (1) IL161357A0 (fr)
WO (2) WO2003035894A2 (fr)

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US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
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US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

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IL161357A0 (en) 2004-09-27
CA2465288A1 (fr) 2003-05-01
EP1438419A2 (fr) 2004-07-21
WO2003035894A3 (fr) 2003-12-04
BR0213520A (pt) 2006-05-23
CA2465274A1 (fr) 2003-05-01
AU2002360305A1 (en) 2003-05-06
CN1610752A (zh) 2005-04-27
EP1438398A2 (fr) 2004-07-21
JP2005507997A (ja) 2005-03-24
WO2003035894A8 (fr) 2004-06-03
WO2003035894A2 (fr) 2003-05-01
WO2003035895A2 (fr) 2003-05-01

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