CA2465274A1 - Analyse de parametres multiples d'acides nucleiques et de caracteristiques morphologiques d'ensemble sur le meme echantillon - Google Patents
Analyse de parametres multiples d'acides nucleiques et de caracteristiques morphologiques d'ensemble sur le meme echantillon Download PDFInfo
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- CA2465274A1 CA2465274A1 CA002465274A CA2465274A CA2465274A1 CA 2465274 A1 CA2465274 A1 CA 2465274A1 CA 002465274 A CA002465274 A CA 002465274A CA 2465274 A CA2465274 A CA 2465274A CA 2465274 A1 CA2465274 A1 CA 2465274A1
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- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Hospice & Palliative Care (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Physiology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne un dosage hautement sensible qui utilise une technique d'amplification amorcée spécifique du gène de bibliothèques d'ARNm issus de cellules rares et de transcrits rares trouvés dans le sang. Ce dosage permet de détecter des ARNm (10 copies /cellule) trouvés dans une à dix cellules isolées via un enrichissement immunomagnétique. Ce dosage constitue une amélioration par rapport à la PCR multiplex et permet de détecter efficacement des séquences de codage rares de cellules de carcinome circulant dans le sang. Ces techniques conviennent pour établir le profil de cellules isolées issues de tissus ou de fluides anatomiques et elles servent d'auxiliaire pour un diagnostic clinique de divers carcinomes, notamment dans la détection au stade précoce et dans la classification des cellules tumorales circulant. La surveillance des profils d'acide nucléique et de protéine de cellules dans des formats soit classiques, soit de jeu ordonné de microéchantillons, facilite la gestion d'intervention thérapeutique comprenant la classification par stades, la surveillance de la réponse à la thérapie, la confirmation de rémission et la détection de régression.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US33066901P | 2001-10-26 | 2001-10-26 | |
| US60/330,669 | 2001-10-26 | ||
| US36994502P | 2002-04-04 | 2002-04-04 | |
| US60/369,945 | 2002-04-04 | ||
| PCT/US2002/034397 WO2003035894A2 (fr) | 2001-10-26 | 2002-10-28 | Analyse de parametres multiples d'acides nucleiques et de caracteristiques morphologiques d'ensemble sur le meme echantillon |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2465274A1 true CA2465274A1 (fr) | 2003-05-01 |
Family
ID=26987390
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002465288A Abandoned CA2465288A1 (fr) | 2001-10-26 | 2002-10-28 | Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon |
| CA002465274A Abandoned CA2465274A1 (fr) | 2001-10-26 | 2002-10-28 | Analyse de parametres multiples d'acides nucleiques et de caracteristiques morphologiques d'ensemble sur le meme echantillon |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002465288A Abandoned CA2465288A1 (fr) | 2001-10-26 | 2002-10-28 | Analyse a parametres multiples d'acides nucleiques complets et de caracteristiques morphologiques sur le meme echantillon |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP1438419A2 (fr) |
| JP (1) | JP2005507997A (fr) |
| KR (1) | KR20040047971A (fr) |
| CN (1) | CN1610752A (fr) |
| AU (1) | AU2002360305A1 (fr) |
| BR (1) | BR0213520A (fr) |
| CA (2) | CA2465288A1 (fr) |
| IL (1) | IL161357A0 (fr) |
| WO (2) | WO2003035894A2 (fr) |
Families Citing this family (62)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6913697B2 (en) | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
| US8986944B2 (en) | 2001-10-11 | 2015-03-24 | Aviva Biosciences Corporation | Methods and compositions for separating rare cells from fluid samples |
| US8980568B2 (en) | 2001-10-11 | 2015-03-17 | Aviva Biosciences Corporation | Methods and compositions for detecting non-hematopoietic cells from a blood sample |
| US7318902B2 (en) | 2002-02-04 | 2008-01-15 | Colorado School Of Mines | Laminar flow-based separations of colloidal and cellular particles |
| ES2375724T3 (es) | 2002-09-27 | 2012-03-05 | The General Hospital Corporation | Dispositivo microflu�?dico para seperación de células y sus usos. |
| CA2542656A1 (fr) * | 2003-10-16 | 2005-05-06 | Genomic Health, Inc. | Systeme d'essai qrt-pcr pour le profilage d'expression genetique |
| US7332597B2 (en) | 2004-06-28 | 2008-02-19 | University Of Kentucky Research Foundation | Primers and probe to identify mycobacterium tuberculosis complex |
| ITBO20040420A1 (it) | 2004-07-07 | 2004-10-07 | Type S R L | Macchina per taglio e formatura di piattine metalliche |
| DE102005013261A1 (de) * | 2005-03-22 | 2006-09-28 | Adnagen Ag | Reagenz und Verfahren zur Verhinderung der zeitabhängigen Expression in biologischen Zellen |
| US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
| DE102005029808A1 (de) * | 2005-06-27 | 2007-01-04 | Siemens Ag | Trägermaterial, Verfahren zu seiner Herstellung und Verwendung |
| ITBO20050481A1 (it) | 2005-07-19 | 2007-01-20 | Silicon Biosystems S R L | Metodo ed apparato per la manipolazione e/o l'individuazione di particelle |
| US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| KR101446626B1 (ko) | 2005-09-02 | 2014-10-06 | 도레이 카부시키가이샤 | 신장암 진단, 신장암 환자 예후 예측을 위한 조성물 및 방법 |
| US20070059683A1 (en) * | 2005-09-15 | 2007-03-15 | Tom Barber | Veterinary diagnostic system |
| US9134237B2 (en) | 2005-09-20 | 2015-09-15 | Janssen Diagnotics, LLC | High sensitivity multiparameter method for rare event analysis in a biological sample |
| US9127302B2 (en) | 2005-09-20 | 2015-09-08 | Janssen Diagnostics, Llc | System for the detection and enumeration of suspect target cells in a mixed cell population |
| ITBO20050646A1 (it) | 2005-10-26 | 2007-04-27 | Silicon Biosystem S R L | Metodo ed apparato per la caratterizzazione ed il conteggio di particelle |
| US9878326B2 (en) | 2007-09-26 | 2018-01-30 | Colorado School Of Mines | Fiber-focused diode-bar optical trapping for microfluidic manipulation |
| US9487812B2 (en) | 2012-02-17 | 2016-11-08 | Colorado School Of Mines | Optical alignment deformation spectroscopy |
| US9885644B2 (en) | 2006-01-10 | 2018-02-06 | Colorado School Of Mines | Dynamic viscoelasticity as a rapid single-cell biomarker |
| US8119976B2 (en) | 2007-07-03 | 2012-02-21 | Colorado School Of Mines | Optical-based cell deformability |
| ITTO20060226A1 (it) | 2006-03-27 | 2007-09-28 | Silicon Biosystem S P A | Metodo ed apparato per il processamento e o l'analisi e o la selezione di particelle, in particolare particelle biologiche |
| CA2647843A1 (fr) * | 2006-04-18 | 2007-10-25 | Wellstat Biologics Corporation | Detection de proteines dans des cellules neoplasiques en circulation |
| US8137912B2 (en) | 2006-06-14 | 2012-03-20 | The General Hospital Corporation | Methods for the diagnosis of fetal abnormalities |
| US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| EP2589668A1 (fr) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Analyse de cellules rares utilisant la division d'échantillons et les marqueurs d'ADN |
| WO2007147074A2 (fr) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Utilisation de génotypage snp fortement parallèle pour diagnostic fœtal |
| EP2041299A4 (fr) | 2006-07-14 | 2010-01-13 | Aviva Biosciences Corp | Procedes et compositions servant a detecter des cellules rares dans un echantillon biologique |
| JP4926812B2 (ja) * | 2007-02-01 | 2012-05-09 | シスメックス株式会社 | 血球分析装置および体液分析方法 |
| US10722250B2 (en) | 2007-09-04 | 2020-07-28 | Colorado School Of Mines | Magnetic-field driven colloidal microbots, methods for forming and using the same |
| ITTO20070771A1 (it) | 2007-10-29 | 2009-04-30 | Silicon Biosystems Spa | Metodo e apparato per la identificazione e manipolazione di particelle |
| LT2334812T (lt) | 2008-09-20 | 2017-04-25 | The Board Of Trustees Of The Leland Stanford Junior University | Neinvazinis fetalinės aneuploidijos diagnozavimas sekvenavimu |
| IT1391619B1 (it) | 2008-11-04 | 2012-01-11 | Silicon Biosystems Spa | Metodo per l'individuazione, selezione e analisi di cellule tumorali |
| US10895575B2 (en) | 2008-11-04 | 2021-01-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
| CA2782123C (fr) | 2009-03-17 | 2017-05-02 | Silicon Biosystems S.P.A. | Dispositif microfluidique |
| JP5729904B2 (ja) | 2009-06-02 | 2015-06-03 | キヤノン株式会社 | 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法 |
| CN102575244B (zh) | 2009-09-17 | 2015-07-22 | Jsr株式会社 | 抗生物素蛋白与生物素衍生物的解离方法以及解离剂 |
| US8722328B2 (en) * | 2010-01-04 | 2014-05-13 | Qiagen Gaithersburg, Inc. | Methods, compositions, and kits for recovery of nucleic acids or proteins from fixed tissue samples |
| US9273347B2 (en) | 2010-09-10 | 2016-03-01 | Bio-Rad Laboratories, Inc. | Detection of RNA-interacting regions in DNA |
| CN102565404B (zh) * | 2010-12-24 | 2014-04-16 | 牛刚 | 一种检测血液中游离大肠癌细胞标志物的试剂盒 |
| CN102156164B (zh) * | 2011-01-13 | 2012-12-12 | 北京肿瘤医院 | 核仁磷酸蛋白可变剪接体的质谱鉴定方法和胃癌诊断试剂盒 |
| CN102109525B (zh) * | 2011-01-26 | 2013-10-16 | 牛刚 | 一种检测血液中游离乳腺癌细胞标志物的试剂盒 |
| WO2012112606A1 (fr) | 2011-02-15 | 2012-08-23 | Bio-Rad Laboratories, Inc. | Détection de méthylation dans une sous-population d'adn génomique |
| US20120252038A1 (en) * | 2011-04-01 | 2012-10-04 | Chianese David A | Steroid receptor assays |
| EP2739752B1 (fr) * | 2011-08-03 | 2017-07-19 | Bio-Rad Laboratories, Inc. | Filtration de petits acides nucléiques à l'aide de cellules perméabilisées |
| ITTO20110990A1 (it) | 2011-10-28 | 2013-04-29 | Silicon Biosystems Spa | Metodo ed apparato per l'analisi ottica di particelle a basse temperature |
| ITBO20110766A1 (it) | 2011-12-28 | 2013-06-29 | Silicon Biosystems Spa | Dispositivi, apparato, kit e metodo per il trattamento di un campione biologico |
| WO2014067528A1 (fr) * | 2012-10-29 | 2014-05-08 | Arcedi Biotech Aps | Enrichissement et détection améliorés de cellules sanguines rares |
| US9857361B2 (en) | 2013-03-15 | 2018-01-02 | Iris International, Inc. | Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples |
| ES2658692T3 (es) | 2013-03-15 | 2018-03-12 | Iris International, Inc. | Método y composición para teñir y procesar una muestra de orina |
| EP3670663A1 (fr) * | 2014-02-28 | 2020-06-24 | Gen-Probe Incorporated | Procédé pour isoler l'acide nucléique d'échantillons dans des conservateurs de cytologie aqueux contenant du formaldéhyde |
| US9771571B2 (en) | 2014-02-28 | 2017-09-26 | Gen-Probe Incorporated | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| US9771572B2 (en) | 2014-02-28 | 2017-09-26 | Gen-Probe Incorporated | Method of isolating nucleic acid from specimens in liquid-based cytology preservatives containing formaldehyde |
| JP2014158468A (ja) * | 2014-03-05 | 2014-09-04 | Clinical Genomics Pty Ltd | 検出方法 |
| KR20170135834A (ko) * | 2015-02-10 | 2017-12-08 | 일루미나, 인코포레이티드 | 세포 성분을 분석하기 위한 방법 및 조성물 |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| US10282588B2 (en) * | 2016-06-09 | 2019-05-07 | Siemens Healthcare Gmbh | Image-based tumor phenotyping with machine learning from synthetic data |
| CN110456034B (zh) * | 2018-05-07 | 2022-07-22 | 上海市第十人民医院 | 一种循环肿瘤细胞的检测方法 |
| CN109402240B (zh) * | 2019-01-08 | 2020-08-25 | 圣湘生物科技股份有限公司 | 核酸释放剂、核酸pcr扩增方法和pcr扩增试剂盒 |
| WO2023080601A1 (fr) * | 2021-11-05 | 2023-05-11 | 고려대학교 세종산학협력단 | Procédé et dispositif de diagnostic de maladie faisant appel à une technologie d'imagerie par ombre sans lentille basée sur l'apprentissage machine |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4929556A (en) * | 1986-02-06 | 1990-05-29 | The Dow Chemical Company | Enzyme immobilization with polysulfonium salts |
| US5346994A (en) * | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| CA2262403C (fr) * | 1995-07-31 | 2011-09-20 | Urocor, Inc. | Biomarqueurs et cibles de diagnostic, de pronostic et de traitement du cancer de la prostate |
| US6329179B1 (en) * | 1996-03-26 | 2001-12-11 | Oncomedx, Inc. | Method enabling use of extracellular RNA extracted from plasma or serum to detect, monitor or evaluate cancer |
| US6156564A (en) * | 1996-06-07 | 2000-12-05 | The United States Of America As Represented By The Department Of Health And Human Services | Cellular apoptosis susceptibility protein (CSP) and antisense CSP |
| IL137802A0 (en) * | 1998-02-12 | 2001-10-31 | Immunivest Corp | A method and kit for detecting rare cells in a mixed cell population |
| US6156515A (en) * | 1999-02-09 | 2000-12-05 | Urocor, Inc. | Prostate-specific gene for diagnosis, prognosis and management of prostate cancer |
| US6358697B2 (en) * | 1999-04-21 | 2002-03-19 | Children's Hospital Medical Center | Intracellular pharmaceutical targeting |
-
2002
- 2002-10-28 CN CNA028261313A patent/CN1610752A/zh active Pending
- 2002-10-28 JP JP2003538395A patent/JP2005507997A/ja not_active Ceased
- 2002-10-28 WO PCT/US2002/034397 patent/WO2003035894A2/fr not_active Application Discontinuation
- 2002-10-28 BR BRPI0213520-5A patent/BR0213520A/pt unknown
- 2002-10-28 CA CA002465288A patent/CA2465288A1/fr not_active Abandoned
- 2002-10-28 KR KR10-2004-7006181A patent/KR20040047971A/ko not_active Application Discontinuation
- 2002-10-28 EP EP02795565A patent/EP1438419A2/fr not_active Withdrawn
- 2002-10-28 CA CA002465274A patent/CA2465274A1/fr not_active Abandoned
- 2002-10-28 WO PCT/US2002/034570 patent/WO2003035895A2/fr not_active Application Discontinuation
- 2002-10-28 EP EP02795554A patent/EP1438398A2/fr not_active Withdrawn
- 2002-10-28 IL IL16135702A patent/IL161357A0/xx unknown
- 2002-10-28 AU AU2002360305A patent/AU2002360305A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003035894A3 (fr) | 2003-12-04 |
| KR20040047971A (ko) | 2004-06-05 |
| WO2003035894A2 (fr) | 2003-05-01 |
| WO2003035895A2 (fr) | 2003-05-01 |
| CN1610752A (zh) | 2005-04-27 |
| WO2003035895A3 (fr) | 2004-01-08 |
| EP1438419A2 (fr) | 2004-07-21 |
| IL161357A0 (en) | 2004-09-27 |
| BR0213520A (pt) | 2006-05-23 |
| CA2465288A1 (fr) | 2003-05-01 |
| AU2002360305A1 (en) | 2003-05-06 |
| JP2005507997A (ja) | 2005-03-24 |
| EP1438398A2 (fr) | 2004-07-21 |
| WO2003035894A8 (fr) | 2004-06-03 |
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| Date | Code | Title | Description |
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| FZDE | Discontinued | ||
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