WO2002055654A2 - Immunosubtraction method for sample preparation for 2-dge - Google Patents

Immunosubtraction method for sample preparation for 2-dge Download PDF

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Publication number
WO2002055654A2
WO2002055654A2 PCT/US2002/000060 US0200060W WO02055654A2 WO 2002055654 A2 WO2002055654 A2 WO 2002055654A2 US 0200060 W US0200060 W US 0200060W WO 02055654 A2 WO02055654 A2 WO 02055654A2
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
receptor
ligand
matrix
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2002/000060
Other languages
English (en)
French (fr)
Other versions
WO2002055654A3 (en
Inventor
Rembert Pieper
N Leigh Anderson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Large Scale Proteomics Corp
Original Assignee
Large Scale Proteomics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Proteomics Corp filed Critical Large Scale Proteomics Corp
Priority to US10/250,898 priority Critical patent/US7642089B2/en
Priority to JP2002556705A priority patent/JP2004523747A/ja
Priority to EP02708939A priority patent/EP1349915A4/en
Publication of WO2002055654A2 publication Critical patent/WO2002055654A2/en
Publication of WO2002055654A3 publication Critical patent/WO2002055654A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N30/14—Preparation by elimination of some components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/468—Flow patterns using more than one column involving switching between different column configurations
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PCT/US2002/000060 2001-01-09 2002-01-09 Immunosubtraction method for sample preparation for 2-dge Ceased WO2002055654A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/250,898 US7642089B2 (en) 2001-01-09 2002-01-09 Immunosubtraction method
JP2002556705A JP2004523747A (ja) 2001-01-09 2002-01-09 ２−ｄｇｅ用試料調製のための免疫サブトラクション法
EP02708939A EP1349915A4 (en) 2001-01-09 2002-01-09 IMMUNOSUBTRACTION PROCESS FOR SAMPLE PREPARATION FOR 2-DGE

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US26021701P 2001-01-09 2001-01-09
US60/260,217 2001-01-09
US09/977,358 2001-10-16
US09/977,358 US7691645B2 (en) 2001-01-09 2001-10-16 Immunosubtraction method

Publications (2)

Publication Number Publication Date
WO2002055654A2 true WO2002055654A2 (en) 2002-07-18
WO2002055654A3 WO2002055654A3 (en) 2002-09-19

Family

ID=26947809

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000060 Ceased WO2002055654A2 (en) 2001-01-09 2002-01-09 Immunosubtraction method for sample preparation for 2-dge

Country Status (4)

Country Link
US (2) US7691645B2 (https=)
EP (1) EP1349915A4 (https=)
JP (1) JP2004523747A (https=)
WO (1) WO2002055654A2 (https=)

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WO2004019009A1 (en) 2002-08-23 2004-03-04 Royal Women's Hospital Depletion of plasma proteins
WO2004072647A1 (en) * 2003-02-07 2004-08-26 Beyond Genomics Removal of proteins from a sample
AU2004205158B1 (en) * 2003-08-21 2005-02-10 Macquarie University Detecting molecules
WO2005019243A1 (en) * 2003-08-21 2005-03-03 Macquarie University Detecting molecules
WO2006072140A1 (en) * 2005-01-06 2006-07-13 Macquarie University Detecting molecules
AU2003250621B2 (en) * 2002-08-23 2007-08-09 Inex Innovations Exchange Pte Ltd Depletion of plasma proteins
WO2007024825A3 (en) * 2005-08-26 2008-01-10 Genway Biotech Inc Immunoaffinity separation and analysis compositions and methods
WO2008058325A1 (en) * 2006-11-13 2008-05-22 Healthlinx Limited Immunoaffinity compositions for immunodepletion of proteins
US7396468B2 (en) 2004-10-01 2008-07-08 Agilent Technologies, Inc. Methods and system for protein separation
JP2008536821A (ja) * 2005-03-23 2008-09-11 バイオ−ラッド ラボラトリーズ インコーポレーティッド タンパク質を精製するための方法
US7943046B2 (en) 2004-10-01 2011-05-17 Agilent Technologies, Inc Methods and systems for on-column protein delipidation
AU2006204591B2 (en) * 2005-01-06 2011-08-04 Macquarie University Detecting molecules
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Cited By (22)

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Publication number Priority date Publication date Assignee Title
AU2003250621B2 (en) * 2002-08-23 2007-08-09 Inex Innovations Exchange Pte Ltd Depletion of plasma proteins
AU2003250621C1 (en) * 2002-08-23 2010-03-11 Inex Innovations Exchange Pte Ltd Depletion of plasma proteins
WO2004019009A1 (en) 2002-08-23 2004-03-04 Royal Women's Hospital Depletion of plasma proteins
US9417218B2 (en) 2002-08-23 2016-08-16 Therapeuticsmd, Inc. Depletion of plasma proteins
EP1540304A4 (en) * 2002-08-23 2006-10-04 Royal Women S Hospital ENHANCEMENT OF PLASMA PROTEINS
WO2004072647A1 (en) * 2003-02-07 2004-08-26 Beyond Genomics Removal of proteins from a sample
WO2005019243A1 (en) * 2003-08-21 2005-03-03 Macquarie University Detecting molecules
US8298783B2 (en) * 2003-08-21 2012-10-30 Macquarie University Detecting molecules
AU2004205158B1 (en) * 2003-08-21 2005-02-10 Macquarie University Detecting molecules
US7396468B2 (en) 2004-10-01 2008-07-08 Agilent Technologies, Inc. Methods and system for protein separation
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US7642089B2 (en) 2010-01-05
US7691645B2 (en) 2010-04-06
JP2004523747A (ja) 2004-08-05
EP1349915A4 (en) 2008-02-27
US20040115725A1 (en) 2004-06-17

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