WO2002024949A1 - Procede d'analyse d'acide nucleique - Google Patents
Procede d'analyse d'acide nucleique Download PDFInfo
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- WO2002024949A1 WO2002024949A1 PCT/JP2000/006526 JP0006526W WO0224949A1 WO 2002024949 A1 WO2002024949 A1 WO 2002024949A1 JP 0006526 W JP0006526 W JP 0006526W WO 0224949 A1 WO0224949 A1 WO 0224949A1
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- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Definitions
- the present invention relates to a method for analyzing a nucleic acid, and a kit for performing the method.
- the method of the present invention includes an extraction step, a detection step, and, in some cases, an amplification step for a nucleic acid to be analyzed, and in each step, the number of internal standard nucleic acids that can be identified and detected from the nucleic acid to be analyzed is reduced.
- At least one type is processed simultaneously with the nucleic acid to be analyzed, and the success or failure of each step is determined based on the detection result of the internal standard nucleic acid, and reanalysis is performed based on the determination result.
- genes have been isolated and identified from living organisms and tissues for gene function analysis.
- PCR polymerase chain reaction
- Science: Vol. 239, pp. 487-491 (1988) has been developed, and it is possible to isolate and detect genes with very low abundance. became.
- human genome analysis is progressing rapidly, and attention has been focused on the association between gene mutations such as single nucleotide polymorphisms and diseases or drug responses.
- Such polymorphisms are expected to be used not only in molecular biology and genetics but also in order-made therapy or medicine in the field of medical diagnosis.
- a typical gene analysis step includes a step of extracting a nucleic acid to be analyzed from a sample and a detection step, and may further include a nucleic acid amplification step between the extraction step and the detection step.
- a widely known method for extracting nucleic acids is a protein such as protease K.
- Add white matter-degrading enzymes and detergents to break down cells and degrade proteins to release nucleic acids add phenolic nocroform and centrifuge to disperse the nucleic acid into the aqueous phase, and then collect water Solubilization of nucleic acids by adding ethanol, isopropanol, etc. to the phase, the so-called proteases K / pheno method, Molecular cloning: A Laooratory manual Appendix E3 to E4 (New York: Cold Spring Harbor Laboratory ⁇ 1989) is there.
- Methods used in the nucleic acid detection step include, for example, single-stranded DNA higher-order polymorphism (SSCP) using gel electrophoresis or restriction fragment length polymorphism. (RFLP), a single-stranded nucleic acid immobilized on a glass silicon wafer or various beads, and a microarray method, a DNA chip method, a bead array method for detecting hybridization with the target nucleic acid, a pi mouth sequencing method, One-by-one, mass spectrometry, and HPLC methods have been developed and widely used.
- SSCP single-stranded DNA higher-order polymorphism
- RFLP restriction fragment length polymorphism.
- nucleic acid amplification methods include polymerase chain reaction (PCR) (US Pat. Nos. 4,683,195 and 4,683,202), nucleic acid sequence-based amplification (NASB A) (European patents 0, 329) , 822) are known and used. Furthermore, nucleic acid quantification methods using competitive amplification or co-amplification are generally known. Have been.
- PCR polymerase chain reaction
- NNB A nucleic acid sequence-based amplification
- nucleic acid quantification methods using competitive amplification or co-amplification are generally known. Have been.
- Such conventional techniques mainly determine the amount of the target nucleic acid by simultaneously amplifying a certain amount of detectable nucleic acid at the same time as the nucleic acid to be analyzed by discriminating from the nucleic acid to be analyzed, which is generally referred to as an internal standard nucleic acid, in the nucleic acid amplification step. It is premised that nucleic acids to be analyzed are normally prepared (Tokuhei 8-107798, Tokuhyo 11-123095, Tokuhyo 11-506613). On the other hand, Japanese Patent Application Laid-Open No.
- 8-501222 describes a method for reflecting the extraction efficiency of the target nucleic acid in the extraction step and a method for detecting cross-contamination between samples, but it is essential to include a nucleic acid amplification step. Therefore, it was difficult to determine the success or failure of a series of nucleic acid analysis steps and / or in which step an abnormality occurred.
- the present invention provides a method for assuring or improving the reliability of analysis results in nucleic acid analysis.
- An object of the present invention is to provide a target nucleic acid analysis method. Disclosure of the invention
- the present invention relates to a method for analyzing a nucleic acid to be analyzed and another step in a nucleic acid analysis method, which further comprises a step of extracting and detecting a nucleic acid to be analyzed, and optionally an amplification step between the extraction step and the detection step.
- a nucleic acid analysis method which further comprises a step of extracting and detecting a nucleic acid to be analyzed, and optionally an amplification step between the extraction step and the detection step.
- one or more types of internal standard nucleic acids that can be identified and detected from the internal standard nucleic acid to be detected are added to the sample, and then each step is performed in the same container as the target nucleic acid to detect nucleic acids.
- a method for judging the success or failure of each step from the detection result of each internal standard nucleic acid in the step is provided.
- the present invention provides a nucleic acid analysis method including a nucleic acid analysis target extraction step and a detection step, wherein the nucleic acid is extracted after adding one or more types of internal standard nucleic acids for extraction that can be identified and detected from the nucleic acid to be analyzed. And determining the success or failure of the extraction step from the detection result of the internal standard nucleic acid in the detection step.
- a nucleic acid analysis method including a nucleic acid analysis target extraction step and a detection step, wherein the nucleic acid is extracted after adding one or more types of internal standard nucleic acids for extraction that can be identified and detected from the nucleic acid to be analyzed. And determining the success or failure of the extraction step from the detection result of the internal standard nucleic acid in the detection step.
- the present invention relates to a nucleic acid analysis method including an extraction step and a detection step of a nucleic acid to be analyzed,
- Performing the extraction step and the detection step after adding the nucleic acid to be analyzed and at least one kind of internal standard nucleic acid for extraction and the internal standard nucleic acid for detection, which are mutually distinguishable and detectable; and the internal standard nucleic acid obtained by the detection step Provided is a method for analyzing a nucleic acid, comprising determining the success or failure of an extraction step and a detection step from the detection results of the above.
- the present invention relates to a nucleic acid analysis method including an extraction step, an amplification step and a detection step of a nucleic acid to be analyzed,
- the present invention provides a nucleic acid analysis method comprising an extraction step and a detection step of a nucleic acid to be analyzed
- step (b) After adding at least one or more types of internal standard nucleic acids for detection, which can be detected and distinguished from the nucleic acid to be analyzed and the internal standard nucleic acids for extraction, to the sample containing the nucleic acids extracted in the above step (a) A nucleic acid detection step of detecting nucleic acid, and
- a nucleic acid analysis method is provided.
- the present invention relates to a nucleic acid analysis method including an extraction step, an amplification step and a detection step of a nucleic acid to be analyzed, (a) A sample containing the nucleic acid to be analyzed can be amplified at the same time as the nucleic acid to be analyzed, but after adding at least one internal standard nucleic acid for extraction that can be identified and detected from the nucleic acid to be analyzed, the nucleic acid is added to the sample. Nucleic acid extraction step to be extracted,
- the sample containing the nucleic acid extracted in the step (a) can be amplified simultaneously with the nucleic acid to be analyzed and the internal standard nucleic acid for extraction, but the nucleic acid to be analyzed and the internal standard nucleic acid for extraction
- nucleic acid detection step of detecting the nucleic acid after adding the detection internal standard nucleic acid
- a nucleic acid analysis method is provided.
- the nucleic acid analysis method of the present invention is characterized in that the analysis is performed again from one of the steps based on the result of the success / failure determination of the extraction step, the amplification step, or the detection step.
- a fixed amount of an internal standard nucleic acid for extraction having at least one base sequence different from that of the nucleic acid to be analyzed is added to the sample containing the nucleic acid to be analyzed, and then the nucleic acid extraction step is performed.
- a known amount of the internal standard nucleic acid for extraction is added to the obtained nucleic acid extract by adding a certain amount of the internal standard nucleic acid for detection having at least one base sequence different from that of the extracted nucleic acid, and then discriminating and detecting nucleic acids having one or more base sequence difference A nucleic acid detection step is performed.
- the success or failure of each process is determined as shown in Fig. 1, and reanalysis is determined based on the determination results.
- Case 3 (When the detection result of the internal standard nucleic acid for extraction is abnormal or not detected, and the detection result of the internal standard nucleic acid for detection is normal): It is considered that the nucleic acid extraction process was not performed normally, and the Judge and execute again from "Re-execution 1" shown in Fig. 2. In particular, when no nucleic acid to be analyzed is detected, the possibility of false negative is considered to be extremely high. Further, when the nucleic acid to be analyzed is detected in the sample to be analyzed, the mechanism of adding the internal standard nucleic acid for extraction and the internal standard nucleic acid for extraction may be abnormal. Replace or check the addition mechanism of the internal standard nucleic acid for extraction, or perform both of them, and repeat from “Re-execution 1” shown in FIG.
- the nucleic acid extraction step used in the present invention includes the above-described protease K-phenol method, a method utilizing the characteristic of nucleic acid adsorption to a solid phase, such as magnetic silica particles, silica particles, silica fibers or filters, and the like.
- a known method such as a nucleic acid extraction method using a spin column or a microplate or the like can be used as appropriate, but is not particularly limited thereto.
- known methods include single-stranded DNA higher-order structure polymorphism (SSCP) or restriction fragment length polymorphism (RFLP) using gel electrophoresis, microarray method, DNA Chip method, bead array method, pi-mouth sequence method, incubator method, mass spectrometry method, HPLC method, etc. are available as long as they can identify and detect nucleic acids having at least one base sequence or more.
- the detection signal of the internal standard nucleic acid for extraction or the internal standard nucleic acid for detection may be used for various data processing such as determination of the success or failure of each step, quantification of the target nucleic acid, base length measurement or correction of the detection signal. It is also possible.
- the nucleic acid detection step includes a pretreatment of a sample necessary for nucleic acid detection, for example, a heat modification treatment, various labeling treatments, a solvent exchange, and the like.
- a pretreatment of a sample necessary for nucleic acid detection for example, a heat modification treatment, various labeling treatments, a solvent exchange, and the like.
- the addition of the internal standard nucleic acid for detection to the nucleic acid extract is not necessary. It is desirable to be performed before processing.
- a sample containing the nucleic acid to be analyzed can be amplified simultaneously with the nucleic acid to be analyzed,
- a nucleic acid extraction step is performed by adding one or more kinds of internal standard nucleic acids for extraction, each having a sequence different from each other by at least one base.
- amplification can be performed at the same time as the nucleic acid to be analyzed, but a certain amount of amplification that differs from the nucleic acid to be analyzed and the internal standard nucleic acid for extraction by at least one base or more.
- One or more kinds of internal standard nucleic acids are added to the obtained nucleic acid extract, and then a nucleic acid amplification step is performed. Next, at least one kind of a certain amount of a detection internal standard nucleic acid having a sequence different from the nucleic acid to be analyzed, the extraction internal standard nucleic acid, and the amplification internal standard nucleic acid by at least one base is added to the obtained nucleic acid amplification product. Thereafter, a known nucleic acid detection step capable of discriminating and detecting a nucleic acid having one or more bases different in sequence is carried out. ⁇
- the success or failure of each step is determined in the same manner as described above, and reanalysis is determined based on the determination result. Also, by simultaneously processing a blank (control sample) containing no nucleic acid to be analyzed with the nucleic acid to be analyzed, false positives due to contamination can be monitored and used for determination of reanalysis. In this case, contamination occurs when the target nucleic acid is detected in the blank, and it is necessary to perform the analysis again after the washing operation regardless of the results of the internal standard nucleic acid for extraction and the internal standard nucleic acid for detection. .
- PCR polymerase chain reaction
- NASBA nucleic acid sequence standard amplification
- the amounts of the internal standard nucleic acid for extraction and the internal standard nucleic acid for amplification that are amplified simultaneously with the nucleic acid to be analyzed can be made small compared to the internal standard nucleic acid for detection, but it is preferable. Relative ratios differ and are not specified depending on the amplification and detection methods used.
- the nucleic acid analysis operation of the nucleic acid to be analyzed is determined to be “successful”.
- nucleic acid to be analyzed If the nucleic acid to be analyzed is not detected as a blank, it is highly likely that the nucleic acid analysis operation for the nucleic acid to be analyzed was performed normally. There is a possibility that the nucleic acid addition mechanism is abnormal. Therefore, the internal standard nucleic acid for amplification is replaced, or the addition mechanism of the internal standard nucleic acid for amplification is checked, or both are performed, and the process is performed again from “re-execution 2” shown in FIG.
- the nucleic acid to be analyzed is not detected as a blank, it is highly likely that the nucleic acid analysis operation on the nucleic acid to be analyzed was performed normally.
- the internal standard nucleic acid for amplification and / or the internal standard nucleic acid for amplification There is a possibility that the addition mechanism and the addition mechanism of the internal standard nucleic acid for detection and the internal standard nucleic acid for detection or the internal standard nucleic acid for detection may be abnormal. Therefore, the addition mechanism of the internal standard nucleic acid for amplification and Z or the internal standard nucleic acid for amplification and the addition mechanism for the internal standard nucleic acid for detection and / or the internal standard nucleic acid for detection are exchanged or inspected. Re-execute from “Re-execute 2” shown in 2.
- the extraction internal standard There is a possibility that the addition mechanism of the nucleic acid and / or the internal standard nucleic acid for extraction is abnormal, and the exchange of the internal standard nucleic acid for extraction is checked, or the addition mechanism of the internal standard nucleic acid for extraction is checked, or both are checked. And re-execute from “Re-execution 1” shown in Figure 2.
- the mechanism for adding the internal standard nucleic acid for extraction and / or the internal standard nucleic acid for extraction and the addition of the internal standard nucleic acid for detection and z or the internal standard nucleic acid for detection There is a possibility that the mechanism may be abnormal.
- Case 7 (When the detection result of the internal standard nucleic acid for extraction and the internal standard nucleic acid for amplification is abnormal or undetected, and the detection result of the internal standard nucleic acid for detection is normal): The amplification step and the nucleic acid extraction step It was not performed normally, and it was judged as “extracted amplification failure”, and the process was repeated from “Re-execution 1J” shown in Fig. 2. If the nucleic acid to be analyzed was detected in the sample to be analyzed, the internal standard nucleic acid for the extraction was used. And / or the mechanism for adding the internal standard nucleic acid for extraction and the mechanism for adding the internal standard nucleic acid for amplification and Z or the internal standard nucleic acid for amplification may be abnormal.
- the internal standard nucleic acid is added in a fixed amount, that is, at a known concentration, various data processing such as quantification of the target nucleic acid and base length measurement / correction of the detection signal are performed based on the amount of the detection signal of the internal standard nucleic acid. It is possible to do.
- a method including a primer sequence identical to the nucleic acid to be analyzed, which is widely known as a common primer pair, is included in the internal standard nucleic acid.
- a primer sequence identical to the nucleic acid to be analyzed which is widely known as a common primer pair
- the present invention can be practiced using a plurality of independent individual primer pairs.
- the nucleotide sequence of the internal standard nucleic acid must differ from the target nucleic acid by at least one base.However, when the amplification step is included or when a DNA chip using hybridization is used for the detection step, the target nucleic acid differs from the target nucleic acid. The higher the similarity, the easier it is for the amplification efficiency and the coupling efficiency to become the same, so that the reproducibility is excellent and it is suitable.
- the present invention also provides a kit for performing the above-described nucleic acid analysis method of the present invention
- a detection reagent containing the internal standard nucleic acid for detection and Z or the internal standard nucleic acid for detection Provide a kit characterized by including:
- the present invention further provides a kit for performing the above nucleic acid analysis method
- each internal standard nucleic acid may be included alone in the kit, or may be included together with other reagents required in each step, such as a solvent, a labeling reagent, and a primer. good.
- reagents such as a solvent, a labeling reagent, and a primer. good.
- Each component that can be included in the reagent can be easily recognized by those skilled in the art, and is not particularly limited.
- the internal standard nucleic acid for extraction is a fixed amount of nucleic acid having a sequence different from the nucleic acid to be analyzed by at least one base
- the internal standard nucleic acid for detection is It is characterized in that each of the internal standard nucleic acids for use is a certain amount of nucleic acid having a sequence different from at least one base.
- the kit includes an amplification internal standard nucleic acid and an amplification reagent containing an internal standard nucleic acid for amplification or amplification
- the internal standard nucleic acid for extraction can be amplified simultaneously with the nucleic acid to be analyzed.
- a certain amount of nucleic acid having a sequence at least one base or more different from the nucleic acid to be analyzed, and the internal standard nucleic acid for amplification can be simultaneously amplified with the nucleic acid to be analyzed, but the nucleic acid to be analyzed and the internal standard nucleic acid for extraction At least one nucleotide whose sequence differs by at least one base, and wherein the internal standard nucleic acid for detection is at least one base relative to each of the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, and the internal standard nucleic acid for amplification. It is characterized by a certain amount of nucleic acid having a different upper sequence.
- the nucleic acid analysis method of the present invention is carried out using the kit of the present invention, it is possible to use a detection method capable of discriminating a nucleic acid having a sequence different from one or more bases. From the detection results of the standard nucleic acid, it becomes possible to perform various data processing such as quantification of the nucleic acid to be analyzed, base length measurement, and correction of the detection signal.
- FIG. 1 shows a success / failure determination table for each step in the method of the present invention including an extraction step and a detection step.
- FIG. 2 shows a flowchart of the nucleic acid analysis method of the present invention.
- FIG. 3 shows a success / failure determination table of each step in the method of the present invention including an extraction step, an amplification step, and a detection step.
- FIG. 4 shows an example of the internal standard nucleic acid for extraction and the internal standard nucleic acid for detection of the present invention.
- FIG. 5 shows an example of the internal standard nucleic acid for extraction, the internal standard nucleic acid for amplification, and the internal standard nucleic acid for detection of the present invention.
- FIG. 6 shows an example of a detection result in the method of the present invention which is performed to detect a single-stranded DNA higher-order structural polymorphism using gel electrophoresis in the detection step of the present invention.
- FIG. 7 shows an example showing, as a quantitative analysis, a detection result in the method of the present invention using a DNA chip in the detection step.
- FIG. 8 shows an example of data processing for detecting a single-stranded DNA higher-order structural polymorphism using gel electrophoresis in the detection step in the method of the present invention.
- FIG. 2 shows a flowchart illustrating an embodiment of the present invention.
- samples containing nucleic acids to be analyzed such as human blood or other body fluids, tissues or cells, bacteria, viruses, etc.
- Extraction test containing internal standard nucleic acid for extraction and Z or internal standard nucleic acid for extraction After the drug is added to the sample, a step of simultaneously extracting the nucleic acid to be analyzed and the internal standard nucleic acid for extraction is performed,
- the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, and the internal standard nucleic acid for detection are respectively identified. Then, the success or failure of each step is determined in accordance with the above-mentioned case from the detection result of each internal standard nucleic acid, and the re-execution is performed from the extraction step or the nucleic acid detection step based on the determination result.
- a step of simultaneously amplifying the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, and the internal standard nucleic acid for amplification is performed.
- the nucleic acid to be analyzed After adding an internal standard nucleic acid for detection and / or a detection reagent containing the internal standard nucleic acid for detection to the amplified nucleic acid, the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, the internal standard nucleic acid for detection, and the internal standard nucleic acid for amplification are added. Implement the process of identifying and detecting
- each step is determined from the detection results of each internal standard nucleic acid according to the above-mentioned case, and the extraction step, amplification step, or detection step is performed again based on the determination results.
- FIG. 4 shows an example of the internal standard nucleic acid used in the present invention. Specifically, a fixed amount of an internal standard nucleic acid for extraction having a sequence different from the nucleic acid to be analyzed by at least one base is added to a sample containing the nucleic acid to be analyzed, and then the nucleic acid extraction step is performed. A certain amount of the internal standard nucleic acid for detection, which differs from the internal standard nucleic acid for extraction by at least one base, is added to the obtained nucleic acid extract. It is an example of the internal standard nucleic acid used at the time of carrying out the output step.
- the nucleotide length of the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, and the internal standard nucleic acid for detection are the same, and the thymine (T) at a specific position in the nucleotide sequence of the nucleic acid to be analyzed is adenine (A);
- the nucleic acid was used as an internal standard nucleic acid for detection (IC for detection).
- the extraction IC and the detection IC are nucleic acids having base sequences that differ by two bases, and are detected as different signals by a detector capable of discriminating and detecting one or more bases having different sequences.
- FIG. 5 shows an example of an internal standard nucleic acid used in a nucleic acid analysis method including a nucleic acid amplification step between the extraction step and the detection step.
- the method of the present invention can amplify simultaneously with the nucleic acid to be analyzed, but a certain amount of the internal standard nucleic acid for extraction is different from the nucleic acid to be analyzed by at least one base or more. And then perform a nucleic acid extraction step.
- a certain amount of the internal standard nucleic acid for amplification which can be amplified simultaneously with the nucleic acid to be analyzed and differs from the nucleic acid to be analyzed and the internal standard nucleic acid for extraction by at least one base, is added to the obtained nucleic acid extract. Then, a nucleic acid amplification step is performed. Then, a certain amount of the internal standard nucleic acid for detection, which differs from the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, and the internal standard nucleic acid for amplification by at least one nucleotide, is added to the obtained nucleic acid amplification product, and then one or more nucleotides are added.
- a nucleic acid detection step capable of discriminating and detecting nucleic acids having different sequences is performed. Furthermore, in the amplification step, a method was used in which the internal standard nucleic acid includes a primer sequence identical to the nucleic acid to be analyzed, which is widely known as a means for amplifying the internal standard nucleic acid simultaneously with the target nucleic acid, which is known as a common primer pair. It is an example.
- a nucleic acid having a base sequence in which T at a specific position in the nucleic acid base sequence to be analyzed is different from A by one base is defined as an internal standard nucleic acid for extraction (IC for extraction), and A nucleic acid having a base sequence in which A at another specific position differs from C by one base is defined as an internal standard nucleic acid for amplification (IC for amplification), A nucleic acid having a base sequence in which C at another specific position in the nucleic acid base sequence to be analyzed differs from A by one base is defined as an internal standard nucleic acid for detection (IC for detection).
- the extraction IC, the amplification IC, and the detection IC each have a nucleic acid having a base sequence different from each other by two bases, and different signals are detected by a detector capable of discriminating and detecting a nucleic acid having one or more bases different in sequence.
- a detector capable of discriminating and detecting a nucleic acid having one or more bases different in sequence.
- the primer—region A and primer region B present on both sides of the region having a difference in the nucleotide sequence described above have the same nucleotide sequence as the nucleic acid to be analyzed and each internal standard nucleic acid, so that one kind of primer pair Can amplify each base sequence.
- FIG. 6 shows an example of the results obtained when the nucleic acid analysis method of the present invention is performed.
- Figure 6 shows that the sample containing the nucleic acid to be analyzed has a certain amount of the internal standard nucleic acid for extraction, which differs in sequence by at least one base from the nucleic acid to be analyzed, and that the nucleic acid to be analyzed and the internal standard nucleic acid for extraction have at least one base or more.
- Single-stranded DNA higher-order structural polymorphism using gel electrophoresis as a nucleic acid detection process that can identify and detect nucleic acids with one or more base sequences using different fixed amounts of internal standard nucleic acid for detection
- the extraction IC, the target nucleic acid, and the detection IC each have a predetermined peak intensity, and an example of the chromatogram when the analysis process is performed normally. It is.
- the case of “abnormal 1” is an example of case 3 shown in FIG.
- the fluorescence peak of the extraction IC is extremely small and the extraction IC is abnormal, and the fluorescence peak of the detection IC shows the same fluorescence intensity at the same detection time as in the normal case, so it is normal. Therefore, it is determined that the extraction is defective, and re-execution 1 shown in Fig. 2 is performed.
- the case of “abnormal 2” is an example of case 4 shown in FIG.
- the fluorescence peaks of extraction I and detection IC broaden, and extraction IC and detection IC are not undetected but abnormal.
- the fluorescence of the nucleic acid to be analyzed is Since the peak is also broadened, it is considered that the ability to separate nucleic acids by electrophoresis has been reduced.
- FIG. 7 shows an example of the results obtained when the nucleic acid analysis method of the present invention is performed using a DNA chip. Specifically, it can be amplified at the same time as the nucleic acid to be analyzed, but can be amplified at the same time as a certain amount of the internal standard nucleic acid for extraction and the nucleic acid to be analyzed, the sequence of which differs from the nucleic acid to be analyzed by at least one base or more.
- a certain amount of the internal standard nucleic acid for amplification which differs in sequence by at least one base from the nucleic acid to be analyzed and the internal standard nucleic acid for extraction, and at least one base from the nucleic acid to be analyzed, the internal standard nucleic acid for extraction, and the internal standard nucleic acid for amplification
- a DNA chip is implemented as a nucleic acid detection step capable of discriminating and detecting a nucleic acid having one or more bases different in sequence by using a certain amount of an internal standard nucleic acid for detection having a different sequence.
- Probes containing base sequences complementary to the extraction IC 1, the extraction IC 2, the extraction IC 3, the amplification IC, the detection IC, and the target nucleic acid A are immobilized on the DNA chip, respectively.
- extraction IC1, extraction IC2, extraction IC3, amplification IC, detection IC and target nucleic acid A, each labeled with a fluorescent dye were hybridized, and the fluorescence spectrum was measured. Tol was detected with a measurable DNA chip reader.
- the case of “normal” indicates that the peak intensity (0.5, 1.0) for the extraction IC1, extraction IC2, extraction IC3, amplification IC, and detection IC is shown in the table. Or 1.5) was obtained, and the analysis process was performed successfully. Furthermore, when quantifying the target nucleic acid based on the detection signal amount of the internal standard nucleic acid, the nucleic acid concentration is determined based on the fluorescence intensity of the extraction IC1, the extraction IC2, and the extraction IC3 and the known nucleic acid amount. A calibration curve is prepared, and the amount of the target nucleic acid can be determined to be 2.4 ng based on the fluorescence intensity of the target nucleic acid A.
- the case of “abnormal” is an example shown in case 5 of FIG.
- the fluorescence intensity of each of the extraction IC 1, the extraction IC 2, and the extraction IC 3 is 0.1, which is lower than the predetermined fluorescence intensity of 0.5, 1.0, or 1.5, respectively, and the extraction IC is abnormal.
- Other amplification ICs and detection ICs The fluorescence peak is normal because it shows the same intensity as the predetermined fluorescence intensity
- FIG. 8 shows an example of data processing in which the detection signal is corrected based on the detection signal amount of each internal standard nucleic acid. Similar to Fig. 6, this is an example of an electrophoresis micrograph of SSCP using a capillary electrophoresis apparatus as a nucleic acid detection step capable of discriminating and detecting nucleic acids having one or more bases different in sequence.
- the chromatogram shown before the data processing shows a difference in the detection time of the internal nucleic acid in each lane.
- the chromatogram corrected so that the extraction ICs or detection ICs in each lane have the same detection time is the result of the closed mouth mattogram after data processing. is there.
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PCT/JP2000/006526 WO2002024949A1 (fr) | 2000-09-22 | 2000-09-22 | Procede d'analyse d'acide nucleique |
EP00961207.8A EP1319716B1 (en) | 2000-09-22 | 2000-09-22 | Method of analyzing nucleic acid |
US09/958,727 US7790368B1 (en) | 2000-09-22 | 2000-09-22 | Method for analyzing nucleic acid |
JP2002529541A JP3752488B2 (ja) | 2000-09-22 | 2000-09-22 | 核酸解析方法 |
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Cited By (12)
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JP2005180983A (ja) * | 2003-12-17 | 2005-07-07 | Hitachi High-Technologies Corp | 化学分析装置および化学分析用構造体 |
US7217542B2 (en) | 2002-10-31 | 2007-05-15 | Hewlett-Packard Development Company, L.P. | Microfluidic system for analyzing nucleic acids |
US20110039303A1 (en) * | 2007-02-05 | 2011-02-17 | Stevan Bogdan Jovanovich | Microfluidic and nanofluidic devices, systems, and applications |
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- 2000-09-22 WO PCT/JP2000/006526 patent/WO2002024949A1/ja active Application Filing
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Cited By (18)
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US7217542B2 (en) | 2002-10-31 | 2007-05-15 | Hewlett-Packard Development Company, L.P. | Microfluidic system for analyzing nucleic acids |
JP2005180983A (ja) * | 2003-12-17 | 2005-07-07 | Hitachi High-Technologies Corp | 化学分析装置および化学分析用構造体 |
US9752185B2 (en) | 2004-09-15 | 2017-09-05 | Integenx Inc. | Microfluidic devices |
US20110039303A1 (en) * | 2007-02-05 | 2011-02-17 | Stevan Bogdan Jovanovich | Microfluidic and nanofluidic devices, systems, and applications |
US8557518B2 (en) | 2007-02-05 | 2013-10-15 | Integenx Inc. | Microfluidic and nanofluidic devices, systems, and applications |
US9663819B2 (en) | 2009-06-05 | 2017-05-30 | Integenx Inc. | Universal sample preparation system and use in an integrated analysis system |
US9121058B2 (en) | 2010-08-20 | 2015-09-01 | Integenx Inc. | Linear valve arrays |
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US10525467B2 (en) | 2011-10-21 | 2020-01-07 | Integenx Inc. | Sample preparation, processing and analysis systems |
US11684918B2 (en) | 2011-10-21 | 2023-06-27 | IntegenX, Inc. | Sample preparation, processing and analysis systems |
US10865440B2 (en) | 2011-10-21 | 2020-12-15 | IntegenX, Inc. | Sample preparation, processing and analysis systems |
US10191071B2 (en) | 2013-11-18 | 2019-01-29 | IntegenX, Inc. | Cartridges and instruments for sample analysis |
US10989723B2 (en) | 2013-11-18 | 2021-04-27 | IntegenX, Inc. | Cartridges and instruments for sample analysis |
US10961561B2 (en) | 2014-05-21 | 2021-03-30 | IntegenX, Inc. | Fluidic cartridge with valve mechanism |
US10208332B2 (en) | 2014-05-21 | 2019-02-19 | Integenx Inc. | Fluidic cartridge with valve mechanism |
US11891650B2 (en) | 2014-05-21 | 2024-02-06 | IntegenX, Inc. | Fluid cartridge with valve mechanism |
US10690627B2 (en) | 2014-10-22 | 2020-06-23 | IntegenX, Inc. | Systems and methods for sample preparation, processing and analysis |
JP2019509051A (ja) * | 2016-03-24 | 2019-04-04 | バイオファイア・ダイアグノスティクス,リミテッド・ライアビリティ・カンパニー | 定量的増幅方法 |
Also Published As
Publication number | Publication date |
---|---|
JP3752488B2 (ja) | 2006-03-08 |
EP1319716A4 (en) | 2005-01-12 |
JPWO2002024949A1 (ja) | 2004-01-29 |
EP1319716A1 (en) | 2003-06-18 |
US7790368B1 (en) | 2010-09-07 |
EP1319716B1 (en) | 2013-07-03 |
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