WO2001055345A3 - Verfahren zur herstellung eines serumhaltigen kulturmediums für zellen sowie dessen verwendung - Google Patents

Abstract

Es wird ein Verfahren zur Herstellung eines serumhaltigen Kulturmediums für Zellen, insbesondere Human- und Tierzellen, beschrieben, bei dem das Kulturmedium und/oder das Serum mittels einer Ultrfiltrationsmembran gefiltert wird. Vorzugsweise wird die Abreicherungsrate der Filtrationsmembran mittels Viren der Familie Leviviridae bzw. vergleichbar kleiner Bakteriophagen bestimmt. Das so erhaltene Kulturmedium wird zur Kultivierung von natürlichen und rekombinanten Zellen sowie von Hybridomazellen, sowie zur Herstellung von diagnostischen Kontrollseren, insbesondere zur Herstellung von pharmazeutischen und/oder diagnostischen Präparaten verwendet.

Description

lr national Application No

PlT/DE 01/00350

A CLASSIFICATION OF SUBJECT MATTER

IPC 7 C12N5/00 G01N33/96 C12P1/00 //( C12P1/00 , C12R1 : 91 )

According lo International Patent Classihcalion (IPC) or lo bolh national classificalion and IPC

B. F1ELDS SEARCHED

Minimum documentation searched (classitication System tollowed by classitication Symbols)

IPC 7 C12N

Documentation searched olher lhan minimum documenlation to the extent that such documents are included in the Neids searched

Electronic data base consulted dunng Ihe international search (name ot data base and where practical search terms used)

EPO-Internal , WPI Data , PAJ , MEDLINE , CHEM ABS Data , EMBASE , LIFESCIENCES

C. DOCUMENTS CONSIDERED TO BE RELEVANT

Category " Citation ot document wilh indicalion where appropπate ot the relevant passages Relevant to Claim No

JUNGBAUER , A . ET AL : "Cross fl ow 1-3 , 8, 9 di afi l trati on of seru wi th basal medi um suitable for growth of hybri do as , steri l i zati on and protei n reducti on . ADVANCES IN ANIMAL CELL TECHNOLOGY : CELL ENGINEERING , EVALUATION AND EXPLOITATION . " , DEV . BIOL . STAND . , ( 1987) PP . 87-90. MEETING INFO . : 7. GENERAL MEETING 0F ESACT ON ANIMAL CELL TECHNOLOGY : CELL ENGINEERING , EVALUATION AND EXPLOITATION . BADEN (AUSTRIA) . 30 SEP-4 0CT 1985. ISBN : 3-8055-4556-8. , INST . APPL. MICROBIOL . , UNIV . AGRIC , PETER-JORDANSTR . 82 , A-1190 VIENNA, AUSTRIA XP001015674 the whol e document 4-7

7~

Furlher documents are Iisted in the continuation ot box C El Patent tamily members are Iisted in annex

° Special categoπes ot cited documents

"T" later document published after the international tilmg dale or pπority date and not in contlict with the application but

"A" document defming the general State of the art which is not cited to understand the pnnciple or theory underlying Ihe considered to be ot particular relevance invention Ε" earlier document bul published on or after the international "X" document ot particular relevance the claimed invention filing date cannol be considered novel or cannol be considered to "L" document which may throw doubts on prioπty claιm(s) or mvolve an inventive step when the document is taken alone which is cited to establish Ihe publicalion date of another "Y" document of particular relevance the claimed invention citalion or other Special reason (as specified) cannot be considered to mvolve an inventive step when the

O" document reterring to an oral disclosure use exhibilion or document is combined with one or more other such docuother means ments such combination being obvious lo a person skilled "P" document published pπor to the international filing date but in the art later than the prioπty date claimed "&' document member of the same patenl tamily

Date ot Ihe actual completion ot the international search Date ot mailing of the international search report

9 August 2001 22/08/2001

Name and mailing address ot the ISA Authoπzed officer

European Patent Office P B 5818 Patentlaan 2 NL - 2280 HV Ri|swi|k Tel (+31-70) 3 0-20 0 Tx 31 651 epo nl Fax (+31-70) 3*10-3016 Stei n , A

Form PCT ISA/210 (second Sheet) (July W2) In national Application No

PtT/DE 01/00350

C.(Contιnuation) DOCUMENTS CONSIDERED TO BE RELEVANT

Calegory Citation ot document, with indicalion, where appropriale, ol Ihe relevant passages Relevanl to Claim No

DE 44 29 558 A (SANORELL PHARMA GMBH & CO) 4-7 22 February 1996 (1996-02-22) the whole document

DE 41 26 034 C (SANORELL PHARMA GMBH & CO) 6,7 10 September 1992 (1992-09-10) column 1, line 1 - 1 ine 30 colu n 2, line 38 - line 57 column 4, line 19 - line 42 clai 1

DE 24 20 415 A (RES FOUNDATION OF CHILDREN 1,2,8

5 H ) 14 November 1974 ( 1974-11-14 ) The whole document in particular example 1

DATABASE WPI 1,8

Section Ch, Week 198249

Derwent Publications Ltd., London, GB;

Class D16, AN 1982-06697J

XP002174522

6 SU 905 281 A (AS USSR BIOLOG PHYS), 18 February 1982 (1982-02-18) abstract

LEGGETT J.E. ET AL: "Use of serum 8-10 ultrafiltrate in the serum dilution test." JOURNAL OF INFECTIOUS DISEASES, (1989) 160/4 (616-623). ,

XP001015678 the whole document

Form PCT'ISA'210 (conlinuatioπ of second sheet) (July 1992) In* national Vpphcation No

Information on patent family members

F- /DE 01/00350

Patent document Publication Patent family Publication cited in search report date member(s) date

DE 4429558 A 22-02-1996 AT 180672 T 15-06-1999 AU 3252095 A 14-03-1996 O 9605846 A 29-02-1996 DE 59506111 D 08-07-1999 DK 776212 T 29-11-1999 EP 0776212 A 04-06-1997 ES 2135079 T 16-10-1999 GR 3030688 T 30-11-1999

DE 4126034 C 10-09-1992 AT 147274 T 15-01-1997 AU 2473392 A 02-03-1993 CA 2115069 A 18-02-1993 O 9302714 A 18-02-1993 DE 59207860 D 20-02-1997 EP 0599981 A 08-06-1994 US 5731164 A 24-03-1998

DE 2420415 A 14-11-1974 AU 6837174 A 30-10-1975 FR 2227276 A 22-11-1974 JP 50029739 A 25-03-1975 NL 7405707 A 29-10-1974

SU 905281 A 15-02-1982 NONE