Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
  • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
  • B01L3/5021—Test tubes specially adapted for centrifugation purposes
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
  • B04B—CENTRIFUGES
  • B04B5/00—Other centrifuges
  • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
  • B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
  • B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
  • B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted

Definitions

• the present invention relates to a container assembly having a support bridge that is capable of preventing collapse of the container during centrifugation.
• a lid is positioned on top of the container, and the support bridge is disposed between the lid and an upper portion of the container.
• a centrifuge instrument is a device by which liquid samples may be subjected to a centrifugal force.
• Swinging bucket centrifuge systems are well known in the centrifuge art.
• the rotor in such a system is adapted to receive a bucket that hangs from the rotor body. When the rotor is at rest, the bucket hangs in a generally vertical position. When the rotor is accelerated, the bucket swings from its rest position to a horizontal position.
• U.S. Patent No. 5,591,114 to Romanauskas which is incorporated herein by reference, discloses a swinging bucket centrifuge rotor.
• the body of the rotor has at least one pair of confronting planar sidewalls that are circumferentially spaced apart to define a generally axially extending slot.
• Each planar sidewall has a trunnion pin mounted thereon, and the trunnion pins as a pair serve to receive a swinging bucket.
• U.S. Patent No. 5,624,370 to Romanauskas which is incorporated herein by reference, discloses a bucket for use in a swinging bucket centrifuge rotor.
• the bucket has a cylindrical body with a pair of planar abutments formed on the body. The abutments are diametrically disposed on the body.
• a slot is formed between each abutment and a portion of the body of the bucket, and each slot has a groove.
• the bucket is installed on a rotor by lowering the bucket onto a pair of rotor trunnion pins such that each trunnion pin is received within a groove defined on a respective abutment.
• a container for use in a swinging bucket centrifuge system and made of a rigid material is generally cylindrical in form.
• a swinging bucket can also define a generally rectangular volume within which a flexible or non-cylindrical container can be held. Such an arrangement is commonly used for holding blood bags for centrifugation of blood.
• the centrifugal force that advantageously serves to separate a liquid sample into its constituent parts also acts upon the container that holds the sample.
• the container must be capable of withstanding this force otherwise it will be disfigured or destroyed. For example, if a blood bag is not substantially full during centrifugation, the unfilled portion of the bag will crease and fold into the remainder of the bag and blood particles can become lodged in a crease. Even in the case of a more rigid container, the structural integrity of the container must be sufficient to ensure that it does not collapse under the stress of centrifugal force.
• a container assembly comprising a container housing, a lid for sealing the container housing, and a support bridge positioned below the lid and about the neck of the container housing, thereby preventing centrifugal forces acting on the lid, and on the neck and shoulder of the container, from collapsing the container during centrifugation.
• the support bridge in one embodiment of the invention, has an aperture for securing the lid and a counter bore defining a flange that engages an edge of the lid.
• a lip formed at either end of the bridge engages a respective edge of a swinging bucket in which the container is disposed during centrifugation.
• the container includes a chamber that can have a non-cylindrical form.
• the chamber can be a bag.
• the present invention permits the use of a container made of an inexpensive, lightweight material.
• Fig. 1 is an exploded top perspective view showing components of a container assembly for use in a centrifuge system in accordance with the present invention
• Fig. 2A is a top plan view of a support bridge in accordance with the present invention.
• Fig. 2B is a front elevational view with a vertical section taken along line A-A of Fig. 2A;
• Fig. 2C is a vertical section taken along line B-B of Fig. 2A;
• Fig. 3A is a top plan view of the container assembly of Fig. 1 shown mounted in a swinging bucket;
• Fig. 3B is a front elevational view with portions in vertical section, of the assembled container assembly of Fig.l shown mounted in a swinging bucket shown in vertical section;
• Fig. 3C is a vertical sectional view taken along line C-C of Fig. 3B;
• Fig. 4 is a horizontal sectional view as would be seen along line C-C of Fig. 3B showing the assembled container assembly of Fig. 1 during a centrifuge operation;
• Fig. 5 is a horizontal sectional view as would be seen along line D-D of Fig. 3B;
• Fig. 6 is a vertical sectional view similar to that of Fig. 3C showing an alternate embodiment of the container assembly of the invention.
• FIG. 1 is an exploded view of a container assembly 5 for use in a centrifuge system in accordance with the present invention.
• the assembly includes a container 10, a support bridge 20, and a lid 50.
• an o-ring 30 and an insert plug 40 can be included.
• Container 10 has a chamber 8, a shoulder 12, and a neck 15 with a threaded surface and an opening through which chamber 8 is accessed.
• Lid 50 has a threaded surface that engages the threads of neck 15.
• o-ring 30 is positioned on the circumference of insert plug 40, which is inserted into the opening of container 10.
• Lid 50 with the assistance of insert plug 40 and o- ring 30, seals container 10.
• Neck 15 could have a threaded interior surface and lid 50 could have a threaded exterior surface, or vice versa.
• the threaded surfaces are not essential, and lid 50 can seal container 10 in any suitable manner.
• support bridge 20 prevents lid 50, insert plug 40 and o-ring 30, and also neck 15 and shoulder 12, from collapsing container 10 when they are subjected to centrifugal forces. It can be made of any material capable of withstanding the centrifugal forces.
• support bridge 20 is a collar, made of polypropylene, disposed about neck 15.
• Figs. 2A through 2C are, respectively, a top planar view, a front sectional view and a side sectional view of the support bridge 20 shown in Fig. 1.
• Support bridge 20 is substantially reverse U-shaped. It has a substantially horizontal portion 27 with an aperture 26 that receives the container lid 50 (Fig. 1) and a counter bore defining a lip or flange 22 that engages an edge of lid 50.
• a vertically sloping portion 28 substantially conforms to the contour of the container shoulder 12
• support bridge 20 allows for container 10 to be made of an inexpensive, lightweight material.
• container 10 can be manufactured of any plastic including polyethyleneterephthalate, polypropylene, or polycarbonate, and its walls can be as thin as 1 millimeter.
• container 10 can be manufactured of any conventional material, including a metal such as stainless steel.
• Support bridge 20 also allows for container 10 to be manufactured by an inexpensive process such as blow molding. Another advantage of support bridge 20 is that it permits chamber 8 to have either a cylindrical or non- cylindrical form. In a preferred embodiment, chamber 8 has a non-cylindrical form that permits a greater volume of material to be centrifuged as shown in the discussion accompanying Fig. 5.
• Figs. 3A through 3C are, respectively, a top planar view, a front sectional view and a side sectional view of the container assembly shown in Fig. 1 held in a swinging bucket 100 for use in a swinging bucket centrifuge system.
• a non-cylindrical bucket such as swinging bucket 100 is sometimes referred to as a "rectangular bucket", although its footprint is not truly a quadrilateral.
• Swinging bucket 100 includes slots 110a and 110b that slide over trunnion pins (Fig. 4, reference 230a and 230b) for mounting on a swinging centrifuge rotor (Fig. 4, reference 300) .
• Swinging bucket 100 can be a solid unit, a basket or merely a frame. In this application it serves as a holder for container 10.
• Fig. 4 is a horizontal sectional view as would be seen along line C-C of Fig. 3B showing the assembled container assembly of Fig. 1 during a centrifuge operation.
• a rotor 200 is adapted for rotational motion within a centrifuge instrument about a vertical axis of rotation 210.
• Rotor 200 includes a pair of radially extending arms 220a and 220b with corresponding trunnion pins 230a and 230b to accommodate swinging bucket 100.
• centrifugal force 240 pushes lid 50, neck 15 and shoulder 12 toward chamber 8 of container 10.
• centrifugal force 240 can be many times the normal force of gravity, placing a tremendous strain on container 10.
• Support bridge 20 is a member positioned between lid 50 and swinging bucket 100 for supporting lid 50, neck 15 and shoulder 12, and preventing centrifugal force 240 from collapsing container 10.
• the support of neck 15 and shoulder 12 is accomplished through the engagement of lid 50 and neck 15.
• the centrifugal force 240 is transferred from lid 50 to swinging bucket 100.
• support bridge 20 has an aperture into which lid 50 is set, a counter bore defining a lip or flange 22 that engages an edge 52 of lid 50, and a lip 24 that engages an edge 102 of swinging bucket 100.
• Fig. 5 is a horizontal sectional view as would be seen along line D-D of Fig. 3B showing the advantage of chamber 8 having a non-cylindrical form.
• a cylinder held within swinging bucket 100 would be limited to having a diameter 400 and therefore, a footprint represented by the non-shaded area 410.
• a non-cylindrical footprint can extend further, beyond diameter 400 into the shaded area 420.
• a cylindrical configuration cannot take advantage of shaded area 420. Accordingly, a non-cylindrical chamber can hold a greater volume than a cylindrical chamber.
• Fig. 6 shows a swinging bucket 100 holding another embodiment of a container assembly of the present invention.
• a container 510 is comprised of a bag 508 and a neck 515 with a threaded surface and an opening through which bag 508 can be accessed.
• neck 515 is ultrasonically welded to bag 508.
• an o-ring 530 is positioned on the circumference of insert plug 540, which is inserted into the opening of container 510.
• Lid 550 has a threaded surface that engages the threads of neck 515.
• Lid 550 with the assistance of insert plug 540 and o-ring 530, seals container 510.
• Neck 515 could have a threaded interior surface and lid 550 could have a threaded exterior surface, or vice versa.
• the threaded surfaces are not essential, and lid 550 can seal container 510 in any suitable manner.
• Support bridge 520 is a member positioned between lid 550 and swinging bucket 100 for supporting lid 550 and preventing centrifugal forces from collapsing container 510. Thus, the centrifugal force is transferred from lid 550 to swinging bucket 100.
• support bridge 520 has an aperture into which lid 550 is set, a counter bore defining a lip or flange 522 that engages an edge 552 of lid 550, and a lip 524 that engages an edge 102 of swinging bucket 100.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Analytical Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Hematology (AREA)
• Clinical Laboratory Science (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Centrifugal Separators (AREA)
• External Artificial Organs (AREA)
• Details Of Rigid Or Semi-Rigid Containers (AREA)

Priority Applications (2)

Applications Claiming Priority (2)

Publications (3)

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Cited By (2)

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• 2000
  • 2000-06-29 US US09/606,983 patent/US6350225B1/en not_active Expired - Lifetime
  • 2000-06-30 EP EP00961985.9A patent/EP1246701B1/en not_active Expired - Lifetime
  • 2000-06-30 WO PCT/US2000/040290 patent/WO2001002255A2/en not_active Ceased
  • 2000-06-30 JP JP2001507709A patent/JP4508506B2/ja not_active Expired - Fee Related

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