WO1998026098B1 - Procede de mesure de concentrations relatives en acides nucleique dans un melange complexe et extraction de sequences specifiques - Google Patents

Procede de mesure de concentrations relatives en acides nucleique dans un melange complexe et extraction de sequences specifiques

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Publication number
WO1998026098B1
WO1998026098B1 PCT/US1997/023173 US9723173W WO9826098B1 WO 1998026098 B1 WO1998026098 B1 WO 1998026098B1 US 9723173 W US9723173 W US 9723173W WO 9826098 B1 WO9826098 B1 WO 9826098B1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
sequence
acid molecules
nucleotides
source
Prior art date
Application number
PCT/US1997/023173
Other languages
English (en)
Other versions
WO1998026098A1 (fr
Filing date
Publication date
Priority claimed from US08/764,191 external-priority patent/US6060240A/en
Application filed filed Critical
Priority to AU56057/98A priority Critical patent/AU5605798A/en
Priority to CA002275549A priority patent/CA2275549A1/fr
Priority to EP97952461A priority patent/EP0948649A1/fr
Publication of WO1998026098A1 publication Critical patent/WO1998026098A1/fr
Publication of WO1998026098B1 publication Critical patent/WO1998026098B1/fr
Priority to US10/053,366 priority patent/US20030049599A1/en
Priority to US10/066,448 priority patent/US20020172965A1/en

Links

Abstract

Cette invention a trait à un procédé d'évaluation comparative de concentrations en séquences spécifiques nucléotidiques dans des échantillons provenant de diverses sources. Elle concerne, plus particulièrement, un procédé faisant intervenir des oligonucléotides liés de manière covalente à un support solide, des billes par exemple, afin d'isoler des séquences spécifiques nucléotidiques marquées dans des mélanges complexes. Les procédés de l'invention permettent d'effectuer des comparaisons quantitatives de la concentration en acide nucléique d'une séquence définie dans une pluralité d'échantillons différents d'acide nucléique, provenant, par exemple, de cellules ou de tissus différents ou de banques génétiques. Les acides nucléiques provenant des échantillons sont marqués de manière que les signaux puissent être différenciés et comparés après hybridation aux oligonucléotides se trouvant sur les billes. Il est possible, au titre de cette invention, de prélever les supports solides porteurs de l'acide nucléique hybride ainsi que d'éluer et d'analyser l'acide nucléique cible. Cette invention a trait, en outre, à un procédé permettant de marquer des clones individuels en provenance d'une banque génomique et ce, afin qu'ils puissent être identifiés uniquement et extraits par hybridation à des billes spécifiques.
PCT/US1997/023173 1996-12-13 1997-12-12 Procede de mesure de concentrations relatives en acides nucleique dans un melange complexe et extraction de sequences specifiques WO1998026098A1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU56057/98A AU5605798A (en) 1996-12-13 1997-12-12 Methods for measuring relative amounts of nucleic acids in complex mixture andretrieval of specific sequences therefrom
CA002275549A CA2275549A1 (fr) 1996-12-13 1997-12-12 Procede de mesure de concentrations relatives en acides nucleique dans un melange complexe et extraction de sequences specifiques
EP97952461A EP0948649A1 (fr) 1996-12-13 1997-12-12 Procede de mesure de concentrations relatives en acides nucleique dans un melange complexe et extraction de sequences specifiques
US10/053,366 US20030049599A1 (en) 1996-12-13 2002-01-18 Methods for negative selections under solid supports
US10/066,448 US20020172965A1 (en) 1996-12-13 2002-02-01 Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/764,191 US6060240A (en) 1996-12-13 1996-12-13 Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US08/764,191 1996-12-13

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
US08/764,191 Continuation-In-Part US6060240A (en) 1996-12-13 1996-12-13 Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US08/764,191 Continuation US6060240A (en) 1996-12-13 1996-12-13 Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US32974799A Continuation-In-Part 1996-12-13 1999-06-10
US33610799A Continuation-In-Part 1996-12-13 1999-06-18

Publications (2)

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WO1998026098A1 WO1998026098A1 (fr) 1998-06-18
WO1998026098B1 true WO1998026098B1 (fr) 1998-08-27

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PCT/US1997/023173 WO1998026098A1 (fr) 1996-12-13 1997-12-12 Procede de mesure de concentrations relatives en acides nucleique dans un melange complexe et extraction de sequences specifiques

Country Status (5)

Country Link
US (2) US6060240A (fr)
EP (1) EP0948649A1 (fr)
AU (1) AU5605798A (fr)
CA (1) CA2275549A1 (fr)
WO (1) WO1998026098A1 (fr)

Families Citing this family (171)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
EP0562047A4 (en) 1990-12-06 1995-11-02 Affymax Tech Nv Sequencing by hybridization of a target nucleic acid to a matrix of defined oligonucleotides
US6881571B1 (en) * 1998-03-11 2005-04-19 Exonhit Therapeutics S.A. Qualitative differential screening
US20030165931A1 (en) * 1998-03-11 2003-09-04 Bruno Tocque Qualitative differential screening
US20020172965A1 (en) * 1996-12-13 2002-11-21 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom
EP2354251B1 (fr) * 1997-04-03 2014-10-15 Life Technologies Corporation Compositions et procédés d'amplification en chaine par polymerase-transcriptase inverse (RT-PCR)
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
DE69841241D1 (de) 1997-04-22 2009-11-26 Life Technologies Corp Verfahren zur herstellung von aslv reverser transkriptase zusammengestellt aus mehreren untereinheiten
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
US6893877B2 (en) * 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
DE69942697D1 (de) * 1998-01-12 2010-09-30 Massachusetts Inst Technology Vorrichtung zur Mikrotestdurchführung
JP3944996B2 (ja) * 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US20030228597A1 (en) * 1998-04-13 2003-12-11 Cowsert Lex M. Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation
CA2335951C (fr) 1998-06-24 2013-07-30 Mark S. Chee Decodage de detecteurs matriciels a microspheres
US7297479B2 (en) * 1998-08-06 2007-11-20 Lucent Technologies Inc. DNA-based analog neural networks
US6185561B1 (en) 1998-09-17 2001-02-06 Affymetrix, Inc. Method and apparatus for providing and expression data mining database
US20040199544A1 (en) * 2000-11-02 2004-10-07 Affymetrix, Inc. Method and apparatus for providing an expression data mining database
US6703228B1 (en) * 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
JP3855492B2 (ja) * 1998-10-08 2006-12-13 株式会社日立製作所 混合核酸断片分析法
WO2000023785A2 (fr) * 1998-10-20 2000-04-27 Ljl Biosystems, Inc. Perfectionnement portant sur des dosages par luminescence
WO2000024939A1 (fr) 1998-10-27 2000-05-04 Affymetrix, Inc. Gestion de la complexite et analyse d'adn genomique
US6265165B1 (en) * 1998-11-12 2001-07-24 The Regents Of The University Of California Methods for EST-specific full length cDNA cloning
US6638760B1 (en) * 1998-11-25 2003-10-28 Pe Corporation (Ny) Method and apparatus for flow-through hybridization
US20020090612A1 (en) * 1999-01-08 2002-07-11 Jonathan M. Rothberg Method of identifying nucleic acids
CA2259745A1 (fr) * 1999-01-19 2000-07-19 Guy Tremblay Generation representative des genomes d'une banque d'oligonucleotides d"armn d'expression (cdnas) et leur utilisation
JP2002537564A (ja) * 1999-02-17 2002-11-05 デルタゲン プロテオミクス， インコーポレイテッド 基質−リガンド相互作用スクリーニング方法
GB9905807D0 (en) * 1999-03-12 1999-05-05 Amersham Pharm Biotech Uk Ltd Analysis of differential gene expression
ATE526580T1 (de) * 1999-03-19 2011-10-15 Life Technologies Corp Methode zum sichten von mutierten zellen
JP3911909B2 (ja) * 1999-06-09 2007-05-09 株式会社日立製作所 Ｄｎａ試料調製方法及びｄｎａ試料調製装置
FR2798392B1 (fr) * 1999-09-13 2005-07-15 Exonhit Therapeutics Sa Marqueurs genetiques de la toxicite, preparation et utilisations
FR2798673B1 (fr) * 1999-09-16 2004-05-28 Exonhit Therapeutics Sa Methodes et compositions pour la detection d'evenements pathologiques
US6958225B2 (en) 1999-10-27 2005-10-25 Affymetrix, Inc. Complexity management of genomic DNA
US7109024B2 (en) * 1999-11-15 2006-09-19 Dr. Chip Biotechnology Inc. Biomolecule-bound substrates
US6458538B1 (en) * 1999-12-14 2002-10-01 Ptc Therapeutics, Inc. Methods of assaying for compounds that inhibit premature translation termination and nonsense-mediated RNA decay
US7332271B2 (en) * 2000-02-18 2008-02-19 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
US20050202461A1 (en) * 2000-03-08 2005-09-15 Getts Robert C. Method for converting generic nucleic acid priming sequences
AU2001289284A1 (en) * 2000-04-04 2001-10-15 Enanta Pharmaceuticals, Inc. Methods for identifying peptide aptamers capable of altering a cell phenotype
TWI220927B (en) * 2000-05-12 2004-09-11 Rong-Seng Chang Method for producing a micro-carrier
US6838244B1 (en) * 2000-05-19 2005-01-04 Monsanto Technology Llc Fluorescent oligonucleotides and uses thereof
ATE445158T1 (de) 2000-06-14 2009-10-15 Vistagen Inc Toxizitätstypisierung unter verwendung von leberstammzellen
US6740751B2 (en) * 2000-07-18 2004-05-25 Board Of Regents, The University Of Texas System Methods and compositions for stabilizing microtubules and intermediate filaments in striated muscle cells
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
EP1978110B1 (fr) * 2000-09-06 2010-05-26 Transnetyx, Inc. Procédé et système assistés par ordinateur pour criblage d'ADN genomique
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
US6372436B1 (en) * 2000-09-14 2002-04-16 The Curators Of The University Of Missouri Method for construction of cDNA libraries enriched in clones corresponding to rare mRNA
US7135278B1 (en) 2000-09-29 2006-11-14 University Of Rochester Method of screening for therapeutics for infectious diseases
WO2002057414A2 (fr) * 2000-10-20 2002-07-25 Expression Diagnostics, Inc. Evaluation du niveau d'expression leucocytaire
US6902933B2 (en) * 2000-11-03 2005-06-07 Regents Of The University Of Michigan Surface transfection and expression procedure
ATE351902T1 (de) * 2000-11-03 2007-02-15 Univ Michigan Oberflächentransfektion und expressionsverfahren
US6897067B2 (en) * 2000-11-03 2005-05-24 Regents Of The University Of Michigan Surface transfection and expression procedure
US7015047B2 (en) * 2001-01-26 2006-03-21 Aviva Biosciences Corporation Microdevices having a preferential axis of magnetization and uses thereof
US20060228730A1 (en) * 2001-04-11 2006-10-12 Rando Robert F Methods for identifying small molecules that bind specific RNA structural motifs
WO2002083837A1 (fr) * 2001-04-11 2002-10-24 Ptc Therapeutics, Inc. Methodes d'identification de petites molecules qui se fixent a des motifs structuraux d'arn specifique
WO2002083822A2 (fr) * 2001-04-13 2002-10-24 Mitrovich Michael J Lubrifiant solide et composition appropriee
WO2002090599A1 (fr) * 2001-05-09 2002-11-14 Genetic Id, Inc. Systeme de microtitration universel
US7026121B1 (en) * 2001-06-08 2006-04-11 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US6905827B2 (en) * 2001-06-08 2005-06-14 Expression Diagnostics, Inc. Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases
US7235358B2 (en) 2001-06-08 2007-06-26 Expression Diagnostics, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20030143612A1 (en) * 2001-07-18 2003-07-31 Pointilliste, Inc. Collections of binding proteins and tags and uses thereof for nested sorting and high throughput screening
US7026123B1 (en) * 2001-08-29 2006-04-11 Pioneer Hi-Bred International, Inc. UTR tag assay for gene function discovery
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
US6686162B2 (en) 2001-12-04 2004-02-03 Quest Diagnostics Investments, Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
US6946245B2 (en) 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
US20030134303A1 (en) * 2001-12-11 2003-07-17 Activx Biosciences, Inc, Adenine nucleotide-binding protein-directed probes, and methods of their synthesis and use
US20030166026A1 (en) * 2002-01-09 2003-09-04 Lynx Therapeutics, Inc. Identification of specific biomarkers for breast cancer cells
US20030170700A1 (en) * 2002-01-09 2003-09-11 Lynx Therapeutics, Inc. Secreted and cell surface polypeptides affected by cholesterol and uses thereof
WO2003067231A1 (fr) * 2002-02-07 2003-08-14 The Regents Of The University Of California Particules a codage optique
US7928287B2 (en) * 2002-02-19 2011-04-19 Pioneer Hi-Bred International, Inc. Methods for large scale functional evaluation of nucleotide sequences in plants
AU2003235199A1 (en) * 2002-05-09 2003-11-11 Medigenes Nucleic acid probes for detection of non-viral organisms
US7291461B2 (en) * 2002-06-21 2007-11-06 Ptc Therapeutics, Inc. Methods for identifying small molecules that modulate premature translation termination and nonsense mRNA decay
WO2004010106A2 (fr) * 2002-07-24 2004-01-29 Ptc Therapeutics, Inc. Methodes destinees a identifier des petites molecules modulant la terminaison de traduction prematuree et la degradation de l'arnm induite par mutation non-sens
US20040018495A1 (en) * 2002-07-24 2004-01-29 Xing-Xiang Li Microparticle based signal amplification for the detection of analytes
US20040024193A1 (en) * 2002-07-26 2004-02-05 Williams Gregg T. Method of detecting and quantifying hepatitis C virus
US8277753B2 (en) * 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
CA2521999A1 (fr) * 2002-12-20 2004-09-02 Biotrove, Inc. Appareil et procede de dosage utilisant des reseaux microfluidiques
AU2003303396A1 (en) 2002-12-23 2004-07-22 Agilent Technologies, Inc. Comparative genomic hybridization assays using immobilized oligonucleotide features and compositions for practicing the same
US7833714B1 (en) * 2003-04-11 2010-11-16 Affymetrix, Inc. Combinatorial affinity selection
WO2004096985A2 (fr) * 2003-04-24 2004-11-11 Mayo Foundation For Medical Education And Research Procedes d'evaluation de la diversite biologique
US7892745B2 (en) 2003-04-24 2011-02-22 Xdx, Inc. Methods and compositions for diagnosing and monitoring transplant rejection
US20050016552A1 (en) * 2003-06-10 2005-01-27 Rubbermaid Commercial Products Llc Smoking-waste receptacle with disposable container
US20050153300A1 (en) * 2004-01-09 2005-07-14 Quest Diagnostics Incorporated Methods and compositions for the detection of mucolipidosis IV mutations
US7217522B2 (en) 2004-02-12 2007-05-15 Campass Genetics Llc Genetic analysis by sequence-specific sorting
US8321138B2 (en) 2005-07-29 2012-11-27 Agilent Technologies, Inc. Method of characterizing quality of hybridized CGH arrays
CA2559171A1 (fr) 2004-03-12 2005-09-29 Biotrove, Inc. Chargement d'ensembles d'echantillons de l'ordre du nanolitre
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
US20080102455A1 (en) * 2004-07-06 2008-05-01 Genera Biosystems Pty Ltd Method Of Detecting Aneuploidy
US20060105453A1 (en) * 2004-09-09 2006-05-18 Brenan Colin J Coating process for microfluidic sample arrays
EP1623996A1 (fr) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Procédé amélioré de selection d'une protéine desirée d'une bibliothéque.
US7645575B2 (en) * 2004-09-08 2010-01-12 Xdx, Inc. Genes useful for diagnosing and monitoring inflammation related disorders
US20080318217A1 (en) 2004-09-14 2008-12-25 Yale University Identification of Gene Associated with Reading Disability and Uses Therefor
US7879569B2 (en) 2004-11-09 2011-02-01 The Trustees Of The University Of Pennsylvania Methods for diagnosis of myelodysplastic syndromes (MDS)
US7407757B2 (en) * 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
WO2006088445A2 (fr) * 2005-02-11 2006-08-24 Southern Illinois University Amorces metaboliques pour la detection de bacteries de diminution de perchlorate et leurs procedes d'utilisation
FR2883298B1 (fr) * 2005-03-17 2009-07-24 Univ Aix Marseille Ii Fragments d'acides nucleiques et methode de detection specifique par identification moleculaire de differentes especes de bacteries du genre acinetobacter
WO2006102497A2 (fr) * 2005-03-22 2006-09-28 The Board Of Trustees Of The Leland Stanford Junior University Procedes et compositions de diagnostic, surveillance et developpement de medicaments destines au traitement de l'atherosclerose
EP1885889A4 (fr) * 2005-05-11 2010-01-20 Expression Diagnostics Inc Procedes de surveillance de l'etat fonctionnel de transplants a l'aide de panels de genes
CN1908189A (zh) * 2005-08-02 2007-02-07 博奥生物有限公司 体外辅助鉴定肠型胃癌及其分化程度的方法与专用试剂盒
US20070202515A1 (en) * 2005-10-12 2007-08-30 Pathologica, Llc. Promac signature application
CA2648580A1 (fr) * 2006-04-07 2008-05-02 Xdx, Inc. Expression d'acide nucleique en reponse aux steroides et prevision de l'activite d'une maladie
US8895711B2 (en) * 2006-04-18 2014-11-25 The Trustees Of The University Of Pennsylvania Mutated ACVR1 for diagnosis and treatment of Fibrodyplasia Ossificans Progressiva (FOP)
US8859752B2 (en) 2006-04-18 2014-10-14 The Trustees Of The University Of Pennsylvania SIRNA-based therapy of Fibrodyplasia Ossificans Progressiva (FOP)
US7993832B2 (en) * 2006-08-14 2011-08-09 Xdx, Inc. Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders
US8148067B2 (en) * 2006-11-09 2012-04-03 Xdx, Inc. Methods for diagnosing and monitoring the status of systemic lupus erythematosus
US20080268440A1 (en) * 2007-04-26 2008-10-30 Liu Timothy Z Biomolecule immobilization on surface via hydrophobic interactions
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
ES2327703B1 (es) * 2008-04-30 2010-07-07 Universidad Del Pais Vasco Metodos y reactivos para la deteccion de salmonella sp.
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
CA2794522C (fr) 2010-04-05 2019-11-26 Prognosys Biosciences, Inc. Tests biologiques a codage spatial
WO2012134813A1 (fr) 2011-03-31 2012-10-04 St. Jude Children's Research Hospital Procédés et compositions pour identifier une maladie résiduelle minimale associée à une leucémie lymphoblastique aiguë
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013067448A2 (fr) 2011-11-03 2013-05-10 Vca Antech Inc. Compositions et procédés de détection de divers organismes infectieux
WO2013095941A1 (fr) 2011-12-19 2013-06-27 Valley Health System Procédés et trousses pour détecter des sujets présentant un risque de cancer
EP3339861B8 (fr) 2012-06-15 2023-11-01 Genesis Theranostix Korlatolt Felelossegu Tarsasag Biomarqueurs utilisés pour prédire ou détecter précocement la pré-éclampsie
US10670610B2 (en) 2012-06-15 2020-06-02 Wayne State University Biomarker test for prediction or early detection of preeclampsia and/or HELLP syndrome
JP6320382B2 (ja) 2012-08-13 2018-05-09 ザ ロックフェラー ユニヴァーシティ メラノーマの処置および診断
CA2890045C (fr) 2012-10-31 2016-11-15 The Rockefeller University Traitement et diagnostic du cancer du colon
DK2917369T3 (en) 2012-11-07 2019-04-15 Univ Yale ASSESSMENT OF THE RISK OF READING AND LANGUAGE DISORDER
EP3013984B1 (fr) 2013-06-25 2023-03-22 Prognosys Biosciences, Inc. Procédés pour déterminer des motifs spatiales de cibles biologiques dans un échantillon
US10745761B2 (en) 2014-06-02 2020-08-18 Valley Health System Method and systems for lung cancer diagnosis
US20180010176A1 (en) * 2015-02-13 2018-01-11 Abhijit Ajit Patel Methods for highly parallel and accurate measurement of nucleic acids
WO2016130868A2 (fr) * 2015-02-13 2016-08-18 Vaccine Research Institute Of San Diego Matériels et méthodes pour analyser des isoformes d'arn dans les transcriptomes
EP3901282B1 (fr) 2015-04-10 2023-06-28 Spatial Transcriptomics AB Analyse de plusieurs acides nucléiques spatialement différenciés de spécimens biologiques
JP6703824B2 (ja) * 2015-11-30 2020-06-03 シスメックス株式会社 細胞選択方法、細胞検出方法、細胞選択装置、および細胞検出装置
RU2654671C2 (ru) * 2016-10-06 2018-05-21 Федеральное государственное бюджетное учреждение науки Институт биологии гена Российской академии наук Способ разделения молекул днк
JP2021504315A (ja) 2017-11-21 2021-02-15 ルジェニクス，インコーポレーテッド 多形及びその使用
WO2019202536A1 (fr) 2018-04-18 2019-10-24 St. Jude Children's Research Hospital Essais de génotypage pour identifier des mutations dans xaf1
CN108660191A (zh) * 2018-04-28 2018-10-16 中国科学院苏州生物医学工程技术研究所 一种基于编码微球反应器的数字化多重核酸检测方法
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3660172A1 (fr) 2018-11-28 2020-06-03 Bioscreening and Diagnostics LLC Procédés de diagnostic de lésions cérébrales traumatiques
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (fr) 2019-05-30 2022-04-06 10X Genomics, Inc. Procédés de détection de l'hétérogénéité spatiale d'un échantillon biologique
EP4055185A1 (fr) 2019-11-08 2022-09-14 10X Genomics, Inc. Agents de capture d'analytes marqués spatialement pour le multiplexage d'analytes
EP4025711A2 (fr) 2019-11-08 2022-07-13 10X Genomics, Inc. Amélioration de la spécificité de la liaison d'un analyte
US11174220B2 (en) 2019-12-13 2021-11-16 Inspirna, Inc. Metal salts and uses thereof
EP3891300B1 (fr) 2019-12-23 2023-03-29 10X Genomics, Inc. Procédés d'analyse spatiale utilisant une ligature à matrice d'arn
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021216708A1 (fr) 2020-04-22 2021-10-28 10X Genomics, Inc. Procédés d'analyse spatiale utilisant un appauvrissement d'arn ciblée
EP4153775A1 (fr) 2020-05-22 2023-03-29 10X Genomics, Inc. Mesure spatio-temporelle simultanée de l'expression génique et de l'activité cellulaire
EP4153776A1 (fr) 2020-05-22 2023-03-29 10X Genomics, Inc. Analyse spatiale pour détecter des variants de séquence
WO2021242834A1 (fr) 2020-05-26 2021-12-02 10X Genomics, Inc. Procédé de réinitialisation d'un réseau
EP4025692A2 (fr) 2020-06-02 2022-07-13 10X Genomics, Inc. Procédés de banques d'acides nucléiques
EP4158054A1 (fr) 2020-06-02 2023-04-05 10X Genomics, Inc. Trancriptomique spatiale pour les récepteurs d'antigènes
WO2021252499A1 (fr) 2020-06-08 2021-12-16 10X Genomics, Inc. Méthodes de détermination de marge chirurgicale et méthodes d'utilisation associées
EP4165207A1 (fr) 2020-06-10 2023-04-19 10X Genomics, Inc. Procédés de détermination d'un emplacement d'un analyte dans un échantillon biologique
WO2021263111A1 (fr) 2020-06-25 2021-12-30 10X Genomics, Inc. Analyse spatiale de la méthylation de l'adn
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
EP4229196A1 (fr) 2020-10-15 2023-08-23 Genentech, Inc. Analyse de criblage de candidats de liaison à une cible multiplexée
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (fr) 2020-12-21 2022-06-30 10X Genomics, Inc. Procédés, compositions et systèmes pour capturer des sondes et/ou des codes à barres
EP4301870A1 (fr) 2021-03-18 2024-01-10 10X Genomics, Inc. Capture multiplex de gène et expression de protéines à partir d'un échantillon biologique
EP4196605A1 (fr) 2021-09-01 2023-06-21 10X Genomics, Inc. Procédés, compositions et kits pour bloquer une sonde de capture sur un réseau spatial

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4973679A (en) * 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US4678814A (en) * 1981-03-30 1987-07-07 California Institute Of Technology Polyacrolein microspheres
US4413070A (en) * 1981-03-30 1983-11-01 California Institute Of Technology Polyacrolein microspheres
DE3329892A1 (de) * 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg Verfahren zur herstellung von oligonucleotiden
US4569774A (en) * 1984-11-13 1986-02-11 Mobil Oil Corporation Polyglycol lubricants comprising trifluoromethane sulfonate
FR2596761B1 (fr) * 1986-04-08 1988-05-20 Commissariat Energie Atomique Derives de nucleosides et leur utilisation pour la synthese d'oligonucleotides
US5824469A (en) * 1986-07-17 1998-10-20 University Of Washington Method for producing novel DNA sequences with biological activity
US5149625A (en) * 1987-08-11 1992-09-22 President And Fellows Of Harvard College Multiplex analysis of DNA
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
US5153117A (en) * 1990-03-27 1992-10-06 Genetype A.G. Fetal cell recovery method
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5639595A (en) * 1990-05-01 1997-06-17 Isis Phamaceuticals, Inc. Identification of novel drugs and reagents
ES2264239T3 (es) * 1990-06-27 2006-12-16 Princeton University Complejo de proteinas p53/p90'.
DE69120821T2 (de) * 1990-08-31 1997-01-23 Univ Minnesota Polyethylenglykol-Derivate für Festphasenanwendungen
US5942389A (en) * 1990-10-19 1999-08-24 Board Of Trustees Of The University Of Illinois Genes and genetic elements associated with sensitivity to cisplatin
US5753432A (en) * 1990-10-19 1998-05-19 Board Of Trustees Of The University Of Illinois Genes and genetic elements associated with control of neoplastic transformation in mammalian cells
US5217889A (en) * 1990-10-19 1993-06-08 Roninson Igor B Methods and applications for efficient genetic suppressor elements
GB9115407D0 (en) * 1991-07-17 1991-09-04 Medical Res Council Normalised pcr method
US5256542A (en) * 1992-03-09 1993-10-26 Tanox Biosystems, Inc. Selecting low frequency antigen-specific single B lymphocytes with correction for background noise
ES2156869T5 (es) * 1992-04-15 2008-07-01 The Johns Hopkins University Sintesis de colecciones diversas y utiles de oligonucleotidos.
US5436142A (en) * 1992-11-12 1995-07-25 Cold Spring Harbor Laboratory Methods for producing probes capable of distingushing variant genomic sequences
JPH09500650A (ja) * 1993-07-26 1997-01-21 ケイ．オー．テクノロジー インコーポレイテッド ガン治療薬の主薬としての遺伝子の異なる対立遺伝子の阻害剤
US5700638A (en) * 1993-08-26 1997-12-23 Washington University Cell death regulator
US5482845A (en) * 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5525490A (en) * 1994-03-29 1996-06-11 Onyx Pharmaceuticals, Inc. Reverse two-hybrid method
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5650501A (en) * 1994-06-02 1997-07-22 Mount Sinai Hospital Corporation Serine/threonine kinase and nucleic acids encoding same
US5807522A (en) * 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5789389A (en) * 1995-03-17 1998-08-04 Board Of Trustees Of University Of Illinois BCL2 derived genetic elements associated with sensitivity to chemotherapeutic drugs
US5690894A (en) * 1995-05-23 1997-11-25 The Regents Of The University Of California High density array fabrication and readout method for a fiber optic biosensor
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5763175A (en) * 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
WO1997027213A1 (fr) * 1996-01-23 1997-07-31 The Board Of Trustees Of The Leland Stanford Junior University Procedes servant a rechercher par criblage des peptides effecteurs et des molecules d'arn transdominants
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
US6060240A (en) * 1996-12-13 2000-05-09 Arcaris, Inc. Methods for measuring relative amounts of nucleic acids in a complex mixture and retrieval of specific sequences therefrom

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