WO2006086209A2 - Analyse genetique par tri specifique de sequences - Google Patents

Analyse genetique par tri specifique de sequences Download PDF

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Publication number
WO2006086209A2
WO2006086209A2 PCT/US2006/003688 US2006003688W WO2006086209A2 WO 2006086209 A2 WO2006086209 A2 WO 2006086209A2 US 2006003688 W US2006003688 W US 2006003688W WO 2006086209 A2 WO2006086209 A2 WO 2006086209A2
Authority
WO
WIPO (PCT)
Prior art keywords
primer
polynucleotides
different
terminator
population
Prior art date
Application number
PCT/US2006/003688
Other languages
English (en)
Other versions
WO2006086209A3 (fr
Inventor
Sydney Brenner
Original Assignee
Compass Genetics, Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/055,187 external-priority patent/US7217522B2/en
Priority claimed from US11/173,465 external-priority patent/US7407757B2/en
Priority claimed from US11/176,927 external-priority patent/US7393665B2/en
Application filed by Compass Genetics, Llc filed Critical Compass Genetics, Llc
Publication of WO2006086209A2 publication Critical patent/WO2006086209A2/fr
Publication of WO2006086209A3 publication Critical patent/WO2006086209A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Abstract

L'invention concerne des méthodes servant à trier des polynucléotides issus d'une population en fonction de caractéristiques de séquences prédéterminées. Dans un aspect, la méthode selon l'invention consiste: à étendre une amorce hybridée à des polynucléotides présentant des caractéristiques de séquences prédéterminées afin d'incorporer un terminateur prédéterminé comprenant une fraction de capture; à capturer les polynucléotides comprenant des amorces étendues au moyen d'un agent de capture se liant spécifiquement à la fraction de capture; et à fusionner les polynucléotides capturés à partir des amorces étendues afin de former une sous-population de polynucléotides présentant les caractéristiques de séquences prédéterminées. Dans un autre aspect, la méthode selon l'invention est mise en oeuvre sur une population de polynucléotides marqués, de sorte que, après qu'une sous-population a été sélectionnée, les membres de cette sous-population puissent être analysés simultanément au moyen des marqueurs uniques sur les polynucléotides pour acheminer des informations analytiques vers un réseau d'hybridation pour un affichage.
PCT/US2006/003688 2005-02-10 2006-02-03 Analyse genetique par tri specifique de sequences WO2006086209A2 (fr)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US11/055,187 2005-02-10
US11/055,187 US7217522B2 (en) 2004-02-12 2005-02-10 Genetic analysis by sequence-specific sorting
US66216705P 2005-03-16 2005-03-16
US60/662,167 2005-03-16
US11/173,465 US7407757B2 (en) 2005-02-10 2005-06-30 Genetic analysis by sequence-specific sorting
US11/173,465 2005-06-30
US11/176,927 US7393665B2 (en) 2005-02-10 2005-07-07 Methods and compositions for tagging and identifying polynucleotides
US11/176,927 2005-07-07

Publications (2)

Publication Number Publication Date
WO2006086209A2 true WO2006086209A2 (fr) 2006-08-17
WO2006086209A3 WO2006086209A3 (fr) 2007-09-13

Family

ID=36793569

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/003688 WO2006086209A2 (fr) 2005-02-10 2006-02-03 Analyse genetique par tri specifique de sequences

Country Status (1)

Country Link
WO (1) WO2006086209A2 (fr)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7635566B2 (en) * 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
WO2013163263A3 (fr) * 2012-04-24 2014-03-06 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US10202608B2 (en) 2006-08-31 2019-02-12 Gen9, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US10308931B2 (en) 2012-03-21 2019-06-04 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US11072789B2 (en) 2012-06-25 2021-07-27 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
US11084014B2 (en) 2010-11-12 2021-08-10 Gen9, Inc. Methods and devices for nucleic acids synthesis
US11702662B2 (en) 2011-08-26 2023-07-18 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030143580A1 (en) * 2001-09-06 2003-07-31 Don Straus Rapid and sensitive detection of molecules
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US20030143580A1 (en) * 2001-09-06 2003-07-31 Don Straus Rapid and sensitive detection of molecules
US20040259118A1 (en) * 2003-06-23 2004-12-23 Macevicz Stephen C. Methods and compositions for nucleic acid sequence analysis

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10202608B2 (en) 2006-08-31 2019-02-12 Gen9, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
EP2395113A1 (fr) * 2007-06-29 2011-12-14 Population Genetics Technologies Ltd. Procédés et compositions d'isolation des variantes de séquence d'acide nucléique
US8241850B2 (en) 2007-06-29 2012-08-14 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US7635566B2 (en) * 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
US10982208B2 (en) 2010-11-12 2021-04-20 Gen9, Inc. Protein arrays and methods of using and making the same
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US11084014B2 (en) 2010-11-12 2021-08-10 Gen9, Inc. Methods and devices for nucleic acids synthesis
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US11702662B2 (en) 2011-08-26 2023-07-18 Gen9, Inc. Compositions and methods for high fidelity assembly of nucleic acids
US10308931B2 (en) 2012-03-21 2019-06-04 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
WO2013163263A3 (fr) * 2012-04-24 2014-03-06 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
US10927369B2 (en) 2012-04-24 2021-02-23 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US11072789B2 (en) 2012-06-25 2021-07-27 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing

Also Published As

Publication number Publication date
WO2006086209A3 (fr) 2007-09-13

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