WO1992012703A1 - Stimulation de la croissance des cheveux par application de phospholipides - Google Patents

Stimulation de la croissance des cheveux par application de phospholipides Download PDF

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Publication number
WO1992012703A1
WO1992012703A1 PCT/US1992/000070 US9200070W WO9212703A1 WO 1992012703 A1 WO1992012703 A1 WO 1992012703A1 US 9200070 W US9200070 W US 9200070W WO 9212703 A1 WO9212703 A1 WO 9212703A1
Authority
WO
WIPO (PCT)
Prior art keywords
hair growth
mammal
phospholipid
hair
area
Prior art date
Application number
PCT/US1992/000070
Other languages
English (en)
Inventor
Sung Lan Hsia
Jin Lin He
Original Assignee
University Of Miami
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Miami filed Critical University Of Miami
Publication of WO1992012703A1 publication Critical patent/WO1992012703A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/66—Phosphorus compounds
    • A61K31/683—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols
    • A61K31/685—Diesters of a phosphorus acid with two hydroxy compounds, e.g. phosphatidylinositols one of the hydroxy compounds having nitrogen atoms, e.g. phosphatidylserine, lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/55—Phosphorus compounds
    • A61K8/553—Phospholipids, e.g. lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth

Definitions

  • the present invention relates, in general, to phospholipids, and, in particular, to a method of stimulating hair growth in mammals which involves the topical application of phospholipids.
  • Phospholipids have long been recognized as essential constituents of biological membranes.
  • phospholipids not only serve as structural elements, but that they also play an important role in regulating cellular functions.
  • prostaglandins are potent regulatory substances that are formed from arachidonic acid released from phosphatidylcholine by the action of phospholipase A 2 found in cytoplasmic membranes.
  • diacylglycerols formed from phospholipids by the action of phospholipase C, play pivotal roles in protein kinase C-mediated transduction of signals across the cell membrane.
  • the inositol phosphates derived from phosphatidylinositol are known to regulate the intracellular movement of calcium ions.
  • Phospholipids have been widely used both in cosmetic and therapeutic preparations as emulsifying and moisturizing agents.
  • phospholipids have been used as components in delivery systems and as membrane fluidizers.
  • the present invention provides a new method for using phospholipids that has applications in the cosmetic and pharmaceutical industries, as well as in the wool and fur industries.
  • the present invention relates to a method of stimulating hair growth in a mammal.
  • the method comprises applying to the area of skin of the mammal where hair growth is sought to be stimulated an amount of a phospholipid sufficient to stimulate hair growth.
  • FIGURE 1 shows the back of a rabbit two weeks after having been shaved and treated, on the right side (upper half in photo) , with a 10% solution of soy lecithin in ethanol containing 0.01% BHT, and, on the left side (control) , with ethanol containing 0.01% BHT.
  • FIGURE 2 shows the back of a rabbit forty days after having been shaved and treated, on the right side (upper half in photo) , with a 33% soy lecithin solution in ethanol containing 0.01% BHT and, on the left side (control) , with ethanol containing 0.01% BHT. The fullness of fur on the treated side is greater than that on the control side.
  • FIGURE 3 shows, in the left half of the Figure, two tassels of rabbit hair obtained from an area of skin treated 40 days earlier with 33% soy lecithin in ethanol containing 0.01% BHT. The average length was 30-35 mm. The two tassels on the right were from a control skin area, treated with ethanol containing 0.01% BHT. The average length was 20-25 mm.
  • FIGURE 4 shows the back of a sheep that was sheared and then shaven.
  • the outlined areas are the 8 test areas to which either a control solution (ethanol containing 0.01% BHT-area E) or a test solution was applied.
  • the present invention relates to a method of stimulating hair growth in a mammal.
  • the method comprises applying to the skin of the mammal a composition that includes, as an active ingredient, at least one type of phospholipid.
  • the phospholipid(s) is present in the composition. together with a pharmaceutically acceptable carrier, in an amount sufficient to stimulate hair growth.
  • Phospholipids suitable for use in the present invention include phosphatidylcholine (lecithin) , phosphotidylinositol, phosphatidylethanolamine and phosphatidylserine, or derivatives thereof such as diacylglycerol, arachidonic acid, and phosphatidic acid.
  • lecithin is used.
  • the phospholipids can be isolated from natural sources (for example, egg yolk, soybean or other oily seed including safflower, sunflower and olive, and brain tissue) or can be produced synthetically. In either case, known techniques can be used for purification of the phospholipids (see, for example, J. of American Oil Chemists Soc. 42:53-56 (1965)).
  • compositions suitable for use in the method to which the invention relates include at least one type of phospholipid, for example, at a concentration of at least 1% (w/v) .
  • the composition takes the form of a solution wherein an alcohol, preferably ethanol (and/or isopropanol) , is used as the pharmaceutically acceptable carrier.
  • an alcohol preferably ethanol (and/or isopropanol)
  • ethanol is preferred, other alcohol and non-alcohol pharmaceutically acceptable carriers can be used so long as their presence does not inhibit penetration of the phospholipid.
  • the composition can take the form of a liquid, in addition, it can take the form of, for example, a cream, a lotion, a gel, or an ointment.
  • Topical application of the composition can be effected, for example, by rubbing the composition onto that portion of the skin where hair growth is sought to be stimulated, or by spraying the composition, in the case of a liquid, or by dipping the target skin area into the composition.
  • the amount and frequency of application of the composition depends on the use, (i.e., treatment of baldness in humans or enhancement of wool or fur growth in animals) and can readily be determined by one skilled in the art without undue experimentation.
  • active ingredient phospholipid
  • other agents can be included in the composition to confer desired properties thereon (for example, perfumes, coloring agents, water, absorption enhancers etc.).
  • compositions and methods of the present invention can be used to enhance wool production in sheep or to improve fur quality in animals, including angora rabbits, goats and camels. Baldness in both men and women can also be treated using the compositions and methods of the invention.
  • New Zealand white rabbits about 6 months old, were allowed free access to Purina Rabbit Chow and water.
  • the rabbits were maintained in the air conditioned facility at the Animal Resources of the University of Miami, with 12-hour light/dark cycles, In one experiment, the backs of 10 New England rabbits were shaved to remove all the visible hair.
  • One side of each shaved back was designated the "treatment" side, while the contralateral side was used as the control.
  • To the treatment side in an area of 6 x 20 cm 2 , demarcated with ink, about 5 ml of a lecithin-alcohol solution (10-50% (w/v)) was applied.
  • To the control side in an area of 6 x 20 cm 2 , 3 or 5 ml of alcohol was applied.
  • the skin in the "treatment” area turned pink and remained pink for several days.
  • the color of the skin in the control area remained unchanged.
  • the hair in the treated area was fuller and more luxuriant (see Figure 2) .
  • the average length of the hair in the treatment area was 3.0 to 3.5 cm contrasted with the control area where the average length of the hair was only 2.0 to 2.5 cm (see Figure 3) .
  • the hair was clipped as close to the skin as possible.
  • the width and length of the area from which the hair was removed was precisely measured in mm with a ruler, and the removed hair was weighed on an analytical balance.
  • the weight of the hair was calculated in mg per cm 2 of skin.
  • 18 days after a rabbit was treated with 25% soy lecithin the weight of hair clipped from an area of 11.2 cm 2 of treated skin was 439 mg (39.20 mg/cm 2 ) as compared to 356 mg (31.79 mg/cm 2 ) for the control area.
  • the sheep's back was sheared and then shaved clean with an electric clipper.
  • the shaved back was divided into 8 test areas, each 10 cm x 10 cm, with Magic Marker as shown in Figure 4.
  • the control area on the sheep's back was topically treated with 3 ml of ethanol containing 0.01% BHT.
  • 3 ml of different test solutions in ethanol containing 0.01% BHT were applied.
  • the solutions were applied slowly in small drops, to allow evaporation of ethanol between drops.
  • a 25% solution of egg lecithin was applied three times at three day intervals.
  • a 25% solution of soy lecithin was applied 3 times at 3 day intervals.
  • the wool in approximately 5 cm x 8 cm of each test area was clipped as close to the skin as possible.
  • the sheep was held steady, while the skin area from which the wool was removed was gently stretched, and the length and width were measured in mm with a ruler.
  • the wool clipped from each test area was carefully collected in a beaker, washed 3 times with 200 ml of acetone and 3 times with 200 ml of chloroform to rid the wool of dirt, grease and contaminants. It was then dried to constant weight at ambient temperature in an air conditioned room over-night.
  • the wool collected from each area was weighed on an analytical balance and the weight in mg per cm 2 was calculated for comparison. The results are shown below in Table 1.
  • Medium Chain Triglycerides The above results show that soy lecithin is more effective than egg lecithin in stimulating wool growth, and multiple applications are more effective than a single application. The results also show that hydrogenated lecithin is ineffective in sheep, or slightly inhibitory.
  • Medium chain triglycerides (MCT) may have a sight stimulatory effect.

Abstract

Procédé de stimulation de la croissance des cheveux chez un mammifère. Le procédé consiste à appliquer sur une surface de la peau du mammifère, où l'on cherche à stimuler la croissance des cheveux, une dose de phospholipides suffisante pour stimuler la croissance.
PCT/US1992/000070 1991-01-15 1992-01-15 Stimulation de la croissance des cheveux par application de phospholipides WO1992012703A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US64130091A 1991-01-15 1991-01-15
US641,300 1991-01-15

Publications (1)

Publication Number Publication Date
WO1992012703A1 true WO1992012703A1 (fr) 1992-08-06

Family

ID=24571793

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1992/000070 WO1992012703A1 (fr) 1991-01-15 1992-01-15 Stimulation de la croissance des cheveux par application de phospholipides

Country Status (2)

Country Link
AU (1) AU1252492A (fr)
WO (1) WO1992012703A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014131919A1 (fr) * 2013-02-28 2014-09-04 Jimeno Martínez Leonardo Produit à usage topique pour le cuir chevelu et son procédé d'élaboration
EP2572700A4 (fr) * 2010-05-19 2016-02-17 Biopid Corp Composition pour la prévention de la perte de cheveux ou la stimulation de la pousse des cheveux
WO2017052271A1 (fr) * 2015-09-25 2017-03-30 ㈜프로스테믹스 Composition pour prévenir ou traiter la perte des cheveux

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673567A (en) * 1984-08-16 1987-06-16 Shionogi & Co., Ltd. Process for preparing liposome composition
US4721612A (en) * 1984-04-12 1988-01-26 The Liposome Company, Inc. Steroidal liposomes
US4897269A (en) * 1984-09-24 1990-01-30 Mezei Associates Limited Administration of drugs with multiphase liposomal delivery system
US4937078A (en) * 1988-08-26 1990-06-26 Mezei Associates Limited Liposomal local anesthetic and analgesic products
US5034228A (en) * 1985-12-11 1991-07-23 Moet-Hennessy Recherche Pharmaceutical composition, in particular dermatological or cosmetic, comprising hydrous lipidic lamellar phases or liposomes containing a retinoid or a structural analogue thereof such as a carotenoid

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4721612A (en) * 1984-04-12 1988-01-26 The Liposome Company, Inc. Steroidal liposomes
US4673567A (en) * 1984-08-16 1987-06-16 Shionogi & Co., Ltd. Process for preparing liposome composition
US4897269A (en) * 1984-09-24 1990-01-30 Mezei Associates Limited Administration of drugs with multiphase liposomal delivery system
US5034228A (en) * 1985-12-11 1991-07-23 Moet-Hennessy Recherche Pharmaceutical composition, in particular dermatological or cosmetic, comprising hydrous lipidic lamellar phases or liposomes containing a retinoid or a structural analogue thereof such as a carotenoid
US4937078A (en) * 1988-08-26 1990-06-26 Mezei Associates Limited Liposomal local anesthetic and analgesic products

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2572700A4 (fr) * 2010-05-19 2016-02-17 Biopid Corp Composition pour la prévention de la perte de cheveux ou la stimulation de la pousse des cheveux
WO2014131919A1 (fr) * 2013-02-28 2014-09-04 Jimeno Martínez Leonardo Produit à usage topique pour le cuir chevelu et son procédé d'élaboration
WO2017052271A1 (fr) * 2015-09-25 2017-03-30 ㈜프로스테믹스 Composition pour prévenir ou traiter la perte des cheveux
KR20170037381A (ko) * 2015-09-25 2017-04-04 (주)프로스테믹스 탈모의 예방 또는 치료용 조성물
KR101897451B1 (ko) 2015-09-25 2018-09-12 (주)프로스테믹스 탈모의 예방 또는 치료용 조성물

Also Published As

Publication number Publication date
AU1252492A (en) 1992-08-27

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