US5175082A - Method of characterizing genomic dna - Google Patents

Method of characterizing genomic dna Download PDF

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US5175082A
US5175082A US07/027,858 US2785887A US5175082A US 5175082 A US5175082 A US 5175082A US 2785887 A US2785887 A US 2785887A US 5175082 A US5175082 A US 5175082A
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dna
locus
sequence
probes
minisatellite
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Alec J. Jeffreys
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Imperial Chemical Industries Ltd
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

Definitions

  • the present invention relates generally to polynucleotides and DNA and RNA probes, their preparation and their use in genetic characterization. Such uses may include for example establishing human, animal or plant origin, and the polynucleotides and probes of the invention may thus find use for example in paternity disputes or forensic medicine or in the prevention, diagnosis and treatment of genetic disorders or predispositions.
  • the present invention is based upon the discovery that informative genetic loci may be identified in the genome by the use of multi-locus probes and that polynucleotides and polynucleotide probes may be prepared each of which is specific for a single such informative genetic locus.
  • hypervariable loci include minisatellites 5' to the insulin gene (Bell, Selby and Rutter, 1982), 3' to the c-Ha-ras1 gene (Capon et al., 1983), type II collagen gene (Stoker et al., 1985) and between the ⁇ 2 and ⁇ 1 globin genes (Goodbourn et al., 1983) as well as the D14S1 locus defined by an anonymous DNA clone derived from the telomeric region of the long arm of chromosome 14 (Wyman and White, 1980; Balazs et al., 1982; Wyman, Wolfe and Botstein, 1984).
  • minisatellites differ substantially in their variability, ranging from only 6 different alleles detected at the collagen hypervariable region (Sykes, Ogilvie and Wordsworth, 1985) to more than 80 at the D14S1 locus (Balazs et al., 1986).
  • the total number of hypervariable loci in the human genome is unknown but is likely to be large. Indeed the human genome might contain at least 1500 hypervariable regions.
  • an informative genetic locus as one at which at least 3 different alleles can be distinguished in any sample of 100 randomly selected unrelated individuals. This is expressed herein by stating that the locus has an allelic variation of at least 3(100).
  • the sample of randomly selected unrelated individuals will be 500, more preferably 1000, and the ability to distinguish at least 3 different alleles in such samples is referred to herein as 3 (500) and 3(1000) respectively. It is essential that the sample of 100, 500 or 1000 randomly selected unrelated individuals is indeed selected at random as it will usually be possible to identify 100, 500 or 1000 individuals who have less than 3 alleles at a given informative genetic locus by screening a sufficiently large number of members of the total population.
  • informative genetic locus as used herein may alternatively be defined as one at which at least 3 different alleles can be distinguished in DNA extracted from any 20 cell lines selected from the following, which cell lines have been deposited with the American Type Culture Collection (ATCC):
  • ATCC American Type Culture Collection
  • a polynucleotide capable of hybridizing to a DNA fragment which contains a minisatellite which is specific as to a particular region or locus in the genome, said region or locus having an allelic variation (as hereinbefore defined) of at least 3(100).
  • which method comprises cloning a DNA fragment identified by hybridisation of fragments of genomic DNA with a polynucleotide probe which is capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus;
  • a polynucleotide capable of hybridizing to a DNA fragment which contains a minisatellite which is specific as to a particular region or locus in the genome, said region or locus having a degree of polymorphism of at least 3 (as hereinbefore defined).
  • the polynucleotide probe comprises, with the inclusion of a labeled or marker component, a polynucleotide comprising at least three tandem repeats (there being on average at least 70% homology between all tandemly repeated sequences) of sequences which are homologous with a minisatellite region of the genome to a degree enabling hybridisation of the probe to a corresponding DNA fragment obtained by fragmenting the sample DNA with a restriction endonuclease, wherein:
  • the repeats each contain a core which is at least 70% homologous with a consensus core region of similar length present in a plurality of minisatellites from different genomic loci;
  • the core is from 6 to 16 nucleotides long;
  • the total number of nucleotides within the repeating unit which do not contribute to the core is not more than 15.
  • the said DNA fragment contains a minisatellite from a minisatellite region or hypervariable locus which region or locus is detectable by a polynucleotide probe comprising at least three tandem repeats of the sequence (including complementary sequences): ##STR1## (wherein T is T or U and (A) may be present or absent) or the sequence (including complementary sequences): ##STR2## (wherein T is T or U).
  • a polynucleotide in isolated or cloned form which comprises a nucleotide sequence which is specific as to a single minisatellite region or hypervariable locus and which is homologous therewith to a degree enabling hybridization of the nucleotide sequence to a corresponding DNA fragment obtained by fragmenting sample genomic DNA with a restriction endonuclease, the said DNA fragment containing a minisatellite from said minisatellite region or hypervariable locus, wherein:
  • said region or locus has an allelic variation (as herein defined) of at least 3(100) and
  • said region or locus is detectable by a polynucleotide probe comprising at least three tandem repeats of the sequence (including complementary sequences): ##STR3## (wherein T is T or U and (A) may be present or absent or the sequence (including complementary sequences): ##STR4## (wherein T is T or U).
  • the present invention also relates to a corresponding polynucleotide in which the aforesaid region or locus has a degree of polymorphism of at least 3 as opposed to an allelic variation of at least 3.
  • polynucleotides of the present invention and polynucleotides prepared by the hereinbefore defined method of the invention are prepared by microbiological reproduction of cloned material or by direct synthesis.
  • the invention includes polynucleotides of DNA, RNA and of any other kind hybridisable to DNA.
  • the polynucleotides as defined above are unlabelled and can be in double stranded (ds) or single stranded (ss) form.
  • the invention includes labelled polynucleotides in ss-form for use as probes as well as their labelled ds-precursors, from which ss-probes can be produced.
  • a polynucleotide probe which comprises a polynucleotide of the present invention as hereinbefore defined or a polynucleotide prepared by a method as hereinbefore defined having a labelled or marker component.
  • a polynucleotide probe which comprises a polynucleotide of the present invention as hereinbefore defined or a polynucleotide prepared by a method as hereinbefore defined having a labelled or marker component.
  • the nucleotide sequence of the polynucleotide will be in single stranded form, but preferably the probe will comprise the polynucleotide wholly in single stranded form.
  • a method of preparing a polynucleotide probe as hereinbefore defined which comprises labelling or marking a polynucleotide of the present invention as hereinbefore defined or a polynucleotide prepared by a method of the present invention as hereinbefore defined.
  • the polynucleotide probes of the present invention are preferably 32 P-radiolabelled in any conventional way, but can alternatively be radiolabelled by other means well known in the hybridisation art for example to give 35 S-radiolabelled probes. They may also be labelled with biotin or a similar species by the method of D C Ward et al, as described in Proceedings of the 1981 ICN-UCLA Symposium on Developmental Biology using Purified Genes held in Keystone, Colo. on Mar. 15-20, 1981 vol. XXIII 1981 pages 647-658 Academic Press; Editor Donald D Brown et al or even enzyme-labelled by the method of A D B Malcolm et al, Abstracts of the 604th Biochemical Society Meeting, Cambridge, England (meeting of Jul. 1, 1983).
  • a method of characterizing a test sample of genomic DNA by reference to one or more controls which comprises fragmenting sample DNA with one or more restriction enzymes which do not cleave to any relevant extent a sequence corresponding to a tandem repeat, probing the DNA fragments with a polynucleotide or polynucleotide probe comprising a nucleotide sequence which is specific as to a single minisatellite region or hypervariable locus and which is homologous therewith to a degree enabling hybridisation of the nucleotide sequence to a corresponding DNA fragment containing a minisatellite from said single minisatellite region or hypervariable locus, detecting hybridised fragments of DNA, and comparing the hybridised fragments with a said control or controls, wherein:
  • said minisatellite region or hypervariable locus has an allelic variation of at least 3(100) and
  • said region or locus is detectable by a polynucleotide probe capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus.
  • the present invention also relates to a corresponding method of characterizing a test sample in which the aforesaid region or locus has a degree of polymorphism of at least 3 as opposed to an allelic variation of at least 3.
  • the method of the present invention is preferably effected by the use of a polynucleotide or a polynucleotide preferred by a method of the present invention or a polynucleotide probe of the invention as hereinbefore defined.
  • sample DNA is human DNA.
  • a region of human, animal or plant DNA at a recognised locus or site is said be hypervariable if it occurs in many different forms e.g. as to length or sequence.
  • a region of human, animal or plant DNA which is comprised of tandem repeats of a short DNA sequence. All repeat units may not needily show perfect identity.
  • a gene or other segment of DNA which shows variability from individual to individual or between a given individuals paired chromosomes is said to be polymorphic.
  • the percentage homology is given by the number of base pairs in A less the number of base pair substitutions, additions or deletions in B which would be necessary in order to give A, expressed as a percentage.
  • the percentage homology between two sequences ATGC and AGC is 75%.
  • Nucleotide (nt) and base pair (bp) are used synonymously. Both can refer to DNA or RNA.
  • the abbreviations C, A, G, T refer conventionally to (deoxy)cytidine, (deoxy)adenosine, (deoxy)guanosine and either deoxythymidine or uridine. It will be appreciated however that the abbreviations A, C, G and T may include other base modified nucleosides capable of base pairing with one of the conventional nucleosides (deoxy)adenosine, (deoxy)cytidine, (deoxy)guanosine and either deoxythymidine or uridine. Such base modified nucleoside include (deoxy) inosine and (deoxy)8-azaguanosine.
  • the tandem repeats may be perfect repeats or imperfect repeats or a mixture of perfect and imperfect repeats. There are preferably at least three repeats and more preferably at least 7 repeats in the probe sequence.
  • probes of the invention which may be used singly or in combination with other locus specific probes either in a sequence of tests or in a single test using a mixture or cocktail of probes may be useful in the following areas:
  • Livestock breeding and pedigree analysis/authentication could include, for example, the routine control and checking of pure strains of animals, and checking pedigrees in the case of litigation involving e.g. race horses and dog breeding). Also to provide genetic markers which might show association with inherited traits of economic importance.
  • the polynucleotides or probes derived therefrom have a potential use in plant breeding.
  • the locus specific probes of the present invention are, however, particularly useful in forensic medicine as demonstrated in Example 3 hereinafter and at least the probe p ⁇ g3 may cosegregate with the heterocellular form of hereditary persistence of foetal haemoglobin.
  • the probes also provide a powerful method for use in paternity testing and related utilities (see utilities 2 and 5 above) as well as in cell chimaerism studies.
  • FIG. 1A shows gel electrophoretic purification of a specified hypervariable DNA fragment from a DNA fingerprint, the fragment being designated therein as "fragment g".
  • FIG. 1B shows the organization of DNA fragment g by means of a restriction map of cloned DNA;
  • FIG. 2 shows the DNA sequence of hypervariable fragment g, particularly highlighting the consensus repeat sequence and its alignment with the "core" sequence of British Patent Publication No. 2,166,445;
  • FIG. 3 shows the Mendelian inheritance of polymorphic DNA fragments detected by p ⁇ g3
  • FIG. 4 shows the distribution of minisatellite allele sizes detected by hybridisation to p ⁇ g3;
  • FIG. 5 shows the screening of ⁇ MS recombinants for locus specific hypervariable DNA probes
  • FIG. 6 shows the consensus repeat sequence units of the ⁇ MS series of minisatellites and of the p ⁇ g3 minisatellite
  • FIG. 7 shows the Mendelian inheritance of the hypervariable locus detected by the probe designated herein as ⁇ MS32;
  • FIG. 8 shows an estimation of the allelic variability at the hypervariable locus detected by the probe designated herein as ⁇ MS43;
  • FIG. 9 shows an analysis of allelic length variation at hypervariable loci in pools of human DNA
  • FIG. 10 shows the segregation of the hypervariable locus detected by the probe designated herein as ⁇ MS32, in a panel of human-rodent somatic cell hybrid DNAs digested with Alu I;
  • FIG. 11 shows an assessment of the sensitivity of locus specific hypervariable probes and their application to the forensic analysis of a double rape/murder case
  • FIG. 12 shows the detection of multiple hypervariable loci in human DNA by hybridisation with pooled minisatellite probes of the present invention.
  • polynucleotides of the invention and the polynucleotides prepared by the processes of the invention and polynucleotide probes of the present invention are capable of hybridizing with fragments of DNA containing a minisatellite from a minisatellite region or hypervariable locus which is identifiable by a polynucleotide probe comprising of any one sequence selected from: ##STR5## (wherein Y is C, T or U, X is G or C, R is A or G and T is T or U) or tandem repeats thereof; or a sequence complementary thereto.
  • polynucleotides of the invention polynucleotides prepared by the processes of the invention and probes of the present invention are capable of hybridizing with fragments of DNA containing a minisatellite from a single specific minisatellite region or hypervariable locus and are thus locus specific.
  • the polynucleotides and probes of the present invention will be locus specific under high stringency hybridization conditions.
  • locus specific is used herein in relation to a polynucleotide or probe to mean that the polynucleotide or probe may be used under hybridization conditions which ensure that the polynucleotide or probe hybridizes to only a single specific locus.
  • locus specific probes of the present invention when used at reduced hybridisation stringencies may be capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus and may thus be used to identify other informative genetic loci.
  • which method comprises cloning a DNA fragment identified by hybridisation of fragments of genomic DNA with a polynucleotide probe as hereinbefore defined and preparing therefrom a polynucleotide capable of hybridizing to a DNA fragment which contains a minisatellite which is specific as to a particular region or locus on the genome, said region or locus having an allelic variation (as herein defined) of at least 3(100);
  • the method being effected under hybridisation conditions effective to render the probe capable of differentiating DNA by reference to more than one polymorphic minisatellite region or hypervariable locus.
  • a polynucleotide in isolated or cloned form, which comprises a nucleotide sequence which is specific as to a single minisatellite region or hypervariable locus and which is homologous therewith to a degree enabling hybridisation of the nucleotide sequence to a corresponding DNA fragment obtained by fragmenting sample genomic DNA with a restriction endonuclease, the said DNA fragment containing a minisatellite from said minisatellite region or hypervariable locus, wherein:
  • said region or locus has an allelic variation (as herein defined) of at least 3(100) and
  • said region or locus is detectable by a locus specific polynucleotide probe as hereinbefore defined.
  • the invention also relates to a corresponding method and to a corresponding polynucleotide in which the said region or locus has a degree of polymorphism of at least 3.
  • the invention further relates to probes comprising such locus specific polynucleotides and to a method of characterizing a test sample of DNA using such polynucleotides and polynucleotide probes.
  • the locus specific polynucleotides and probes of the present invention may be homologous with the minisatellite of the minisatellite containing DNA fragment or less preferably with a sequence flanking the minisatellite.
  • polynucleotides and probes of the present invention comprise tandem repeats of a particular sequence such sequence will in general be repeated at least 3 times and such repeats need not necessarily be exact repeats. Repeats which are not exact are described elsewhere as imperfect repeats. Such repeats will nevertheless be recongizably repeated. In general this will mean that there is on average at least 70% homology between all repeating units. Preferably there is at least 80%, more preferably at least 85%, especially 90% homology between all repeating units. It will be appreciated however that the nucleotide sequence or "repeat unit" need not be repeated at all if desired.
  • n is advantageously at least 5, but preferably at least 10.
  • n is 10 to 40, but in principle n can be any number, even from 1 up to 10,000.
  • any flanking sequences are largely irrelevant. They can be omitted or can be present in any number of nucleotides, for example up to 50,000 although to work with such a long probe would not ordinarily be sensible. Such large polynucleotides may nevertheless serve as carriers from which smaller polynucleotides, especially useful as probes, may be excised. These flanking sequences may form part of either ds-DNA or ss-DNA, even when the repeat sequences are of ss-DNA.
  • polynucleotides and polynucleotide probes of the present invention comprise any one sequence selected from formulae 3 to 9 as hereinbefore defined or a sequence complementary thereto or tandem repeats thereof (there being on average at least 70% homology between all tandemly repeated sequences) or a sequence complementary thereto.
  • One preferred polynucleotide of the present invention thus comprises the sequence of formula 3 as hereinbefore defined or tandem repeats thereof or a sequence complementary thereto.
  • Such a nucleotide sequence is specific as to an autosomal locus located on chromosome 7.
  • Polynucleotides and polynucleotide probes comprising such a sequence are referred to herein as p ⁇ g3.
  • a further preferred polynucleotide of the present invention comprises the sequence of formula 4 as hereinbefore defined or tandem repeat(s) thereof or a sequence complementary thereto.
  • Such a nucleotide sequence is specific as to a locus located on chromosome 1.
  • Polynucleotides and polynucleotide probes comprising such a sequence are referred to herein as ⁇ MS1.
  • a further preferred polynucleotide of the present invention comprises the sequence of formula 5 as hereinbefore defined or tandem repeat(s) thereof, or a sequence complementary thereto.
  • Such a nucleotide sequence is specific as to a locus located on chromosome 7.
  • Polynucleotides and polynucleotide probes comprising such a sequence are referred to herein as ⁇ MS31.
  • a further preferred polynucleotide of the present invention comprises the sequence of formula 6 as hereinbefore defined or tandem repeat(s) thereof or a sequence complementary thereto.
  • Such a nucleotide sequence is specific as to a locus located on chromosome 1.
  • Polynucleotides and polynucleotide probes comprising such a sequence are referred to herein as ⁇ MS32.
  • a further preferred polynucleotide of the present invention comprises a sequence selected from formulae 7 and 8 as hereinbefore defined or tandem repeat(s) thereof or a sequence complementary thereto.
  • Such a nucleotide sequence is specific as to a locus located on chromosome 5.
  • Polynucleotides and polynucleotide probes comprising such sequences are referred to herein as ⁇ MS8.
  • a further preferred polynucleotide of the present invention comprises the sequence of formula 9 as hereinbefore defined or tandem repeat(s) thereof or a sequence complementary thereto.
  • Such a nucleotide sequence is specific as to a locus located on chromosome 12.
  • Polynucleotides and polynucleotide probes comprising such a sequence are referred to herein as ⁇ MS43.
  • a method of establishing the identity or otherwise of the test sample donor with one or more control sample donors in which the control sample(s) are similarly fragmented and a comparison is made of the hybridized fragments from the respective samples.
  • a method of establishing a family connection between the test sample donor and one or more control sample donors in which the control sample(s) are similarly fragmented and a comparison is made of the hybridized fragments from the respective samples.
  • the above-mentioned methods may be effected using the polynucleotides or probes of the present invention either singly or by using at least two polynucleotides or probes of the present invention either in a sequence of tests or in a single test using a mixture of the said polynucleotides or probes.
  • a method for the diagnosis of an inherited disease, abnormality or trait which comprises probing the test sample with at least one polynucleotide or polynucleotide probe as hereinbefore defined, said probe, being associated with a particular inherited disease, abnormality or trait.
  • the probes of the present invention which we have tested all serve as locus-specific probes under high stringency hybridization conditions.
  • the present invention enables informative genetic loci to be identified in the human genome and thereby enables locus specific probes to be prepared, each locus specific probe being specific as to a single informative genetic locus.
  • the degree of individual specificity of genotypes determined by locus-specific probes can be estimated from the heterozygosity H at each locus.
  • the mean allele frequency q at a locus is given by (1-H), and the probability that two randomly-selected individuals share the same genotype is q 2 (2-q).
  • This probability of false association of two individuals varies from 0.02 for ⁇ MS8 ot 0.0002 for ⁇ MS31, and is a conservative estimate in that heterogeneity in q will reduce these probabilities.
  • the cumulative probability of false association for all six minisatellite probes is ⁇ 10 -16 , comparable to the odds that two randomly-selected DNA fingerprints detected by one multilocu probe are identical (see British Patent Application No. 8525252).
  • locus-specific hypervariable probes should prove particularly useful in forensic medicine, as shown by the murder case analysed in FIG. 11C where insufficient DNA was recovered from two of the forensic samples for DNA fingerprint analysis. Semen DNA from both victims showed a common phenotype with probes ⁇ MS1 and ⁇ MS31 different from that of the suspect. From the above discussion, we can calculate the probability that victims X and Y had been sexually assaulted by different unrelated men at 2 ⁇ 10 -7 . Similarly, the chance that the two hypothetical assailants were first-degree relatives, for examples brothers, is 0.07. This provides strong evidence that X and Y were sexually assaulted by the same man, a conclusion which was subsequently confirmed by DNA fingerprint analysis of larger amounts of forensic material.
  • the Mendelian inheritance of the hypervariable DNA fragments also allows them to be used for establishing family relationships in for example paternity disputes.
  • the probability that a child's paternal allele is fortuitously present in a randomly-selected man can be estimated at 2q-q 2 .
  • the cumulative probability of false inclusion of a non-father is ##EQU1## comparable to that obtained using two DNA fingerprint probes.
  • the cumulative probability of false inclusion of a first degree relative of the true father, such as a brother is 0.02.
  • hypervariable locus-specific probes can provide a powerful method for establishing family relationships with the caveat that mutations to new length alleles at these unstable loci could occasionally lead to false exclusions.
  • the mutation rate at these hypervariable loci is not yet known.
  • locus-specificity and sensitivity of these locus-specific probes enable pooled probes to be used to generate multi-locus Southern blot patterns (FIG. 12).
  • These "reconstituted DNA fingerprints" produced by 5 pooled probes are considerably less complex than their counterparts detected by multi-locus probes, and should be more amenable to being encoded digitally following densitometric scanning.
  • the pooled-probe profiles show a substantial (18%) level of fragment sharing between unrelated North European individuals, mainly as a result of electrophoretic comigration of minisatellite fragments from different loci.
  • This Example describes the isolation and properties of a specified minisatellite fragment in a DNA fingerprint.
  • DNA was isolated from white blood cells as described by Jeffreys A J et al, (1985) Nature 316, 76-79 and from an Epstein Barr virus transformed lymphoblastoid cell line (see Nilsson, K et al (1971) Int. J. Cancer 8, 443-450) derived from individual III 9 of the HPFH pedigree described by Jeffreys et al., Am. J. Hum. Genet. 39, 11-24. Restriction digestion and Southern blot hybridizations were performed as described elsewhere in the above-mentioned references of Jeffreys A J et al. Double-stranded DNA probe fragments were isolated by electrophoresis onto DE81 paper as described by Dretzen, G et al (1981) Anal. Biochem.
  • p ⁇ g3 DNA was sonicated, size-selected and shotgun cloned into the Sma I site of M13mp19 [see Yanis-Perron C et al (1985) Gene 33, 103-119].
  • 12 random clones were sequenced by the dideoxynucleotide chain-termination method [see Sanger, F et al (1977) Proc. Nat. Acad. Sci. USA 74, 5463-5467 and Biggin M D et al (1983) Proc. Natl. Acad. Sci. USA 80, 3963-3965].
  • M13 clones containing the 5' and 3' flanking regions were detected by hybridization with 32 P-labelled flanking probes a and b (see FIG. 1 described below) and sequenced to establish the flanking region sequence and the beginning and end points of the minisatellite.
  • FIG. 1 illustrates this purification by gel electrophoresis.
  • DNA from individual III9 in the pedigree described by Jeffreys et al was digested with Sau3A and 6-9 kb fragments collected by preparative gel electrophoresis (fraction 1). These fragments were re-electrophoresed to give fractions 2-5. Aliquots of III9 DNA digested with Sau3A and of each fraction were electrophoresed through a 0.8% agarose gel and Southern blot hybridized with the minisatellite probe 33.15. Fragment g (8.2 kb), which tends to cosegregate with HPFH, is approximately 1000-fold purified in fraction 3.
  • the fragment ⁇ g ⁇ , detected in the Sau 3A digest referred to above, and purified as described to produce an approximately 1000 fold enriched fragment ⁇ g ⁇ fraction was cloned into ⁇ L47.1 as described in the above-mentioned Loenen and Brammar reference, and propagated on P2 lysogenic rec + E. coli to select for recombinant phage.
  • the resulting library was screened by hybridisation to probe 33.15.
  • the four positive plaques obtained were very small (plaques ⁇ 0.1 mm diameter) but could be replated at a low efficiency 0-8 pfu/plaque) on recB, recC E. coli to produce normal-sized plaques (1 mm diameter).
  • the structure of the minisatellite fragment in p ⁇ g3 was determined by restriction mapping (FIG. 1B) and DNA sequencing (FIG. 2).
  • FIG. 1B shows the organization of DNA fragment g.
  • the Sau 3A insert in p ⁇ g3 was mapped with restriction endonuclease AluI (A), DdeI (D), Hae III (H) MboII (M), PstI (P) and Sau3A (S). There are no cleavage sites for HinfI or RsaI.
  • the 7.14 kb insert contains 171 tandem repeats of a 37 bp sequence (see FIG. 2) plus 747 bp flanking DNA.
  • the 5' flanking region contains the beginning of an inverted Alu element (hatched). The origins of unique sequence flanking probes a and b are shown.
  • FIG. 2 shows the DNA sequence of hypervariable fragment g.
  • the sequence of the 5' and 3' flanking regions and the beginning and end of the minisatellite is shown together with the repeat sequences of three random regions (a-c) from the minisatellite.
  • the beginning of the inverted Alu element is shown in underlined uppercase, and several simple sequence regions in the 5' flanking region are underlined.
  • the consensus sequence (con) of the 37 bp minisatellite repeat unit is shown, and differences from this consensus are given for individual repeat units.
  • the second repeat unit in region c contains a HaeIII cleavage site (underlined), and this region therefore spans the internal HaeIII site in the minisatellite (FIG. 1B).
  • the consensus repeat sequence is also shown aligned with the "core" sequence in a similar manner to that in British Paent Publication No. 2,166,445.
  • the clone p ⁇ g3 contains a 6.3 kb minisatellite devoid of restriction sites, except for a single internal HaeIII site.
  • the minisatellite is comprised of 171 repeats of a 37 bp unit which contains the sequence GTGGGCAGG; this sequence corresponds precisely to the most invariant part of the 11-16 bp core sequence previously identified as being shared by several different human minisatellites (see British Patent Publication No. 2,166,445 and FIG. 2 herein).
  • the repeat units are not completely homogeneous; sequence analysis of several randomly-selected regions from within the minisatellite revealed a limited amount of repeat sequence variation.
  • variants including a 4 bp deletion which produces a subset of 33 bp repeat units, are diffused over more than one repeat unit.
  • One variant (an A ⁇ C transversion in region C) creates the unique internal HaeIII site and is therefore probably only found in one repeat unit. The beginning and end repeat units of the minisatellite are noticeably more diverged in sequence than are internal repeats.
  • the minisatellite in p ⁇ g3 is flanked by nonrepeated DNA containing the normal density of restriction sites (FIG. 1B).
  • the beginning of the 5' flanking region is comprised of the head of an inverted Alu element.
  • the remaining 5' and 3' flanking regions, defined by hybridization probes a and b are unique sequence DNA and hybridize only to this locus in the total DNA (data not shown).
  • the 5' flanking region contains a considerable amount of simple sequence DNA [polypurine and (ACC) n ] (FIG. 2).
  • Minisatellite fragment g detects a single polymorphic locus
  • the Sau3A insert from p ⁇ g3 was hybridized in the presence of human competitor DNA to human DNA digested with HinfI, the restriction enzyme routinely used for DNA fingerprinting (FIG. 3).
  • FIG. 3 shows the Mendelian inheritance of polymorphic DNA fragments detected by p ⁇ g3.
  • 8 ⁇ g samples of human DNA were digested with HinfI and electrophoresed through a 0.8% agarose gel.
  • Digests were Southern blot hybridized with the Sau3A insert of p ⁇ g3, in 1 ⁇ SSC at 65° in the presence of 6% polyethylene glycol and 50 ⁇ g/ml alkalisheared human placental competitor DNA, and washed after hybridization in 0.2 ⁇ SSC at 65°.
  • p ⁇ g3 detects a single locus which is heterozygous in all individuals shown. Inheritance of alleles (indicated by letters) is Mendelian.
  • DNA fragments detected by p ⁇ g3 at high stringencies segregate in a Mendelian fashion as alleles of a single locus (FIG. 3).
  • FIG. 4 shows the distribution of minisatellite allele sizes. The length of the minisatellite in the shortest common allele was determined by genomic mapping of this fragment in a homozygote, using flanking single-copy probes a and b (FIG. 1) (data not shown).
  • At least 77 different alleles could be resolved in this population sample, with repeat numbers ranging from 14 to ⁇ 525 per allele.
  • the homozygotes in the population sample were all homozygous for the shortest allele. The above estimates of number of alleles and the mean frequency of rare alleles are limited by gel resolution, and the true number of different length alleles in this sample may be greater.
  • the distribution of allele lengths does not appear to be completely random, but shows evidence of trimodality (FIG. 4), with short (14-16 repeats), medium (41-68 repeats) and long (107-525 repeats) classes of alleles.
  • a bimodal distribution of allele lengths has been previously noted in caucasians for the hypervariable region located 5' to the human insulin gene [see Bell, G I et al (1982) Nature 295, 31-35], though this bimodality is not evident in blacks [Lebo, R V et al (1983) Proc. Nat. Acad. Sci. USA 80, 4804-4812].
  • fragment g demonstrates that the large and highly polymorphic DNA fragments in DNA fingerprinting are in principle amenable to molecular cloning to provide locus-specific probes suitable for studying individual hypervariable regions. While fragment g could be cloned in bacteriophage ⁇ , the resulting clones showed abnormal growth properties on rec + E. coli. This will lead to a depletion of minisatellite clones from conventional amplified human libraries in phage. The cloned minisatellite is unstable both in and on subcloning into pUC13 in recA. E. coli; the final recombinant p ⁇ g3 had lost about 30 repeat units compared with fragment g. The physical map of p ⁇ g3 (FIG. 1) does not therefore completely reflect the organization of fragment g.
  • the cloned DNA fragment has the structure expected for a minisatellite, establishing that fragment g is not derived from a longer satellite DNA. Despite the presence both of a "core" sequence in each repeat unit and part of an Alu sequence in the 5' flanking region, the cloned fragment g acts, in the presence of competitor human DNA, as a locus-specific probe.
  • the failure of p ⁇ g3 to detect efficiently other core-containing minisatellites is due to the additional non-core DNA present in each repeat unit which probably interferes with cross-hybridization to other minisatellites (see British Patent Publication No. 2,166,445).
  • the cloned minisatellite shows extreme length polymorphism, presumably due to allelic variation both in the number of repeat units and in the ratio of 37 and 33 bp repeat types in each allele.
  • This locus is much more polymorphic than most or all of the cloned human hypervariable regions so far characterized including the selection of relatively short cloned minisatellites initially used to define the "core" sequence [see British Patent Publication No. 2,166,445].
  • the heterozygosity at this locus is at least 96.6%, with most homozygotes arising from the short common allele.
  • New alleles at this locus are generated by alteration in the repeat number, either by slippage during DNA replication or by unequal exchange driven by the "core" sequence, a putative recombination signal.
  • the parameter 4N e v ( ⁇ ) where N e is the effective population size and v is the mutation rate per gamete to a new length allele, can be most accurately estimated from the number of different alleles scored in a population sample, using the infinite allele model [see Ewens W J (1972) Theor. Popul. Biol. 3, 87-112].
  • the average length of minisatellite DNA at this locus is 5 kb, and thus the mutation (recombination) rate per kb minisatellite is >4 ⁇ 10 -4 , compared with 10 -4 per kb estimated for other shorter core-containing minisatellites and a mean meiotic recombination rate of 10 -5 per kb for human DNA [see Jeffreys, A J et al (1985) Nature 314, 67-73].
  • the rate of generation of new portions at this minisatellite locus is remarkably high, consistent with the inventor's previous suggestions that these core-rich regions may be recombination hotspots.
  • the mutation rate is relatively low for short alleles, which might explain how the shortest allele has drifted to achieve a significant frequency in the population without being disrupted by unequal exchange during this process.
  • DNA fingerprints have proved a powerful method for individual identification and for establishing family relationships in for example paternity and immigration disputes.
  • the accuracy of this method is determined by the low mean probability x that a band is shared by two randomly-selected individuals.
  • x has been estimated empirically at 0.2 for DNA fingerprint HincI fragments larger than 4 kb.
  • band sharing between individuals that is, where a band in one individual is matched in a second individual by a band of similar mobility and autoradiographic intensity
  • DNA fingerprint probes 33.6 and 33.15 it is possible to score the segration of up to 34 dispersed autosomal loci in large human sibships. For most loci examined, only one of the two alleles is scorable in the set of larger (>4 kb) resolved DNA fingerprint fragments, suggesting that large size differences exist between minisatellite alleles, with many alleles being located in the poorly resolved ( ⁇ 4 kb) region of the DNA fingerprint. This is also seen for the cloned minisatellite; HinfI alleles at this locus vary from 1.7 to 20.4 kb in length, and the allele frequency distribution in FIG. 4 shows that both alleles of this locus would be resolved in the DNA fingerprints of only 40% of individuals, while neither allele would be scorable in 14% of people.
  • Minisatellite probes 33.6 and 33.15 detect together about 60 hypervariable loci, many of which should now be amenable to cloning to provide a bank of highly informative single locus probes, for example ideal for linkage studies in man.
  • Linkage analysis of inherited disorders is also possible in single large families using the entire DNA fingerprint. If a polymorphic fragment is detected which appears to cosegregate with the disease, it is essential that this fragment be cloned to provide a locus-specific probe for extending the linkage analysis to additional affected families.
  • Recombinant phage DNA was digested with Sau3A and the large insert fragment separated from small vector fragments by electrophoresis in low gelling temperature agarose (Sea Plaque).
  • the gel slice containing the insert was dissolved in 3 vol water at 65° and aliquots containing 10 ng insert DNA were labelled with 32 P by random oligonucleotide priming [Feinberg and Vogelstein, (1984) Anal. Biochem. 137, 266-267.
  • Genomic DNA samples were restricted and electrophoresed as described by Jeffreys et al (1986) Am. J. Hum. Genet. 39, 11-24. DNA was transferred to Hybond-N (Amersham), fixed by u/v irradiation and prehybridized at 65° for 5 min in 0.5M sodium phosphate, 7% SDS, 1 mM EDTA (pH7.2) [Church and Gilbert, (1984), Proc. Natl. Acad. Sci. USA 81, 1991-1995.
  • Filters were hybridized overnight with 0.5 ⁇ g/ml 32 P-labelled probe DNA (specified activity ⁇ 10 9 cpm/ug DNA) in the presence of 25 ⁇ g/ml sheared single-stranded human placental DNA competitor. After hybridization, filters were washed at 65° in 40 mM sodium phosphate, 1% SDS (pH7.2) followed by a high stringency wash at 65° in 0.1 ⁇ SSC (15 mM NaCl, 1.5 mM trisodium citrate pH7.0) 0.01% SDS. Filters were autoradiographed from 3 hr to 1 week at -80° in the presence of an intensifier screen.
  • MS recombinant DNA was sonicated and 0.3-0.6 kb fragments size-selected and shotgun cloned into the SmaI site of M13mp19 [Yanis-Perron, Vieira and Messing, (1985), Gene 33, 103-119].
  • Recombinant phage were screened by plaque hybridization with 32 P-labelled MS insert DNA. 10 strongly-positive single-stranded phage DNAs from a given MS recombinant were compared pairwise by the C-test [Winter and Fields, (1980) Nucleic Acids Res. 8, 1965-1974]; most DNAs fell into two complementary C-test groups and were therefore likely to be derived from the long, tandem-repetitive region of the ⁇ MS insert.
  • each ⁇ MS recombinant could act as a locus-specific probe for a hypervariable ministellite
  • the Sau3A insert of each recombinant was hybridized in the presence of human competitor DNA to HinfI digests of three randomly selected individuals, or to AluI digests for ⁇ MS32, the minisatellite tandem repeat units of which contain a HinfI site (FIG. 5). 8 out of 10 of the recombinants hybridized to one or two large variable DNA fragments in each human DNA tested.
  • Four of the recombinants detected by probe 33.6 ( ⁇ MS8, 40, 42 and 47) hybridized to the same pattern of variable DNA fragments and are therefore derived from the same hypervariable locus (FIG. 5 Table 1).
  • FIG. 5 shows an Example of the screening of ⁇ MS recombinants for locus-specific hypervariable DNA probes.
  • 2 ⁇ g samples of DNA from three unrelated individuals were digested with HinfI, eletrophoresed through a 0.8% agarose gel and Southern blot hybridized with 32 p-labelled Sau3A inserts from p ⁇ g3 (Example 1), ⁇ MS40 as herein described in Materials and Methods. Filters were washed after hybridization in 0.1 ⁇ SSC at 65°, and autoradiographed in the presence of an intensifier screen for 1 day (left panel) or 1 week (right panel). Note that ⁇ MS8 and ⁇ MS40 detect the same hypervariable locus, and that on prolonged autoradiography, additional variable DNA fragments are detectable.
  • the remaining recombinants ⁇ MS1, 31, 32 and 43 were derived from different loci which did not include the previously characterised p ⁇ g3 locus (see Example 1 and FIG. 5). Although none of these ⁇ MS recombinants produced DNA ⁇ fingerprints ⁇ of multiple variable fragments, most ⁇ MS probes were seen to cross-hybridize weakly to additional polymorphic DNA fragments on prolonged autoradiographic exposure (FIG. 5).
  • Random segments of insert DNA from each ⁇ MS recombinant were sequenced to determine whether each cloned hypervariable locus consisted of tandem-repetitive minisatellite (see Materials and Methods). All ⁇ MS clones were shown to consist primarily of tandem repeat units ranging in length from 9 bp for ⁇ MS1 to 45 bp for ⁇ MS43 (FIG. 6).
  • the minisatellite in MS8 consists of two mutually-interspersed repeat units 29 and 30 bp long.
  • the consensus repeat sequence of p ⁇ g3 is also shown in FIG. 2. Each repeat sequence is aligned with the core sequence, with matches shown in uppercase, and a new core sequence derived from a comparison of the core with p ⁇ g3, ⁇ MS31 and ⁇ MS32 is also disclosed.
  • minisatellite in ⁇ MS8 consists of an intermingled array of two closely-related but distinct repeat units 29 and 30 bp long (FIG. 6). The end-points of the minisatellites in the ⁇ MS recombinants have not yet been determined.
  • tandem-repetitive minisatellite consisting mainly of a tandem-repetitive minisatellite.
  • the tandem-repeat units of p ⁇ g3, ⁇ MS1, ⁇ MS31 and ⁇ MS32 each contain the core sequence, as expected (FIG. 6).
  • the copies of the core sequence are imperfect, and comparisons of the repeat units of these minisatellites do not clearly define a new core sequence preferentially associated with these large and extremely variable loci. It seems likely instead that a wide range of core derivatives may be associated with large minisatellites. More notably, minisatellites ⁇ MS8 and ⁇ MS43 detected by probe 33.6, which consists of tandem repeats of a diverged version of the core sequence (see British Patent Publication No.
  • FIG. 7 shows the Mendelian inheritance of the hypervariable locus detected by ⁇ MS32.
  • 1 ⁇ g DNA samples from CEPH family 1413 were digested with Alu I and Southern blot hybridised to the 32 P-labelled Sau 3A insert from ⁇ MS31. This family is completely informative at this locus.
  • FIG. 8 provides an estimation of allelic variability at the hypervariable locus detected by MS43. 1 ⁇ g samples of DNA from twenty random-selected English people were digested with HinfI and Southern blot hybridized to ⁇ MS43. All individuals are heterozygous, indicating that the level of heterozygosity at this hypervariable locus is high (H>0.86, p>0.95). A more accurate estimate of heterozygosity may be made by comparing alleles in each individual with alleles in the six nearest neighbors on the autoradiograph to estimate the levels of allele sharing between individuals.
  • the larger allele in individual 5 appears to be present in 7, but not in 2, 3, 4, 6 and 8. Similarly, the smaller allele in 5 is not present in 2-4 or 6-8.
  • FIG. 9 shows an analysis of allelic length variation at hypervariable loci in pools of human DNA. 10 ⁇ g samples of DNA from a single individual (a), from a pool of 15 individuals (b) and from a pool of 150 people (c) were digested with AluI (for ⁇ MS32) or HinfI (for all remaining probes) and Southern blot hybridized with the indicated locus-specific hypervariable probes. All individuals were randomly-selected North Europeans. Individual a was included within pool b, and all pool b individuals were included in pool c. It should be noted that ⁇ MS43 detects a second variable region represented by the short HinfI alleles marked 0; this region has not been further characterized.
  • ⁇ MS8 For the least variable locus, ⁇ MS8, there are two predominant alleles 5.3 and 7.2 kb long plus 7 lower frequency alleles resolvable in the pool of 15 people. That the two predominant alleles are not the result of sampling error in these 15 individuals is shown by their similar prevalence in the pool of 150 individuals, together with a larger spectrum of low frequency alleles. Similarly, ⁇ MS43 (94% heterozygosity) shows 7 major alleles shared by the 15- and 150-individual pools, together with many minor alleles.
  • at least 50 different ⁇ MS32 alleles can be resolved in the pool of 150 individuals; this will be a minimal estimate of the total number of alleles limited by gel electrophoretic resolution.
  • loci detected are extremely variable and indeed are amongst the most polymorphic loci so far isolated from human DNA.
  • hypervariable locus-specific probes hybridize to rodent DNA, and therefore the segregation of these loci could be followed in man-rodent somatic cell hybrids [see FIG. 10 and Table 2 below].
  • the six loci were assigned to four different autosomes, with chromosomes 1 and 7 each bearing two hypervariable loci.
  • FIG. 10 shows the segregation of the hypervariable locus detected by ⁇ MS32 in a panel of human-rodent somatic cell hybrid DNAs digested with AluI. This locus cosegregates with human chromosome 1 (see Table 2 below). Interestingly, the hybrid cell line MOG 2C2 carried two alleles of both ⁇ MS32 and ⁇ MS1 (data not shown). This strongly suggests that both parental copies of human chromosome 1 have been retained in this hybrid, and, since all other positive hybrids contained only a single allele at each of these loci, this provides strong supporting evidence for synteny of ⁇ MS1 and ⁇ MS32. Hybrid F4SC13c112 contains chromosome 1p but is negative for ⁇ MS32; thus ⁇ MS32 is provisionally localized to chromosome 1q.
  • Table 2 shows the chromosome assignment of hypervariable loci in human-rodent somatic cell hybrids.
  • the cloned minisatellites so far localized tend to be derived from the larger human autosomes (Table 1), as expected if they are dispersed randomly over the human genome. In the absence of precise regional localization, it remains possible that these minisatellites are preferentially located at centromeres and telomeres, which are rich in simple sequence satellite DNA. However, segregation analysis of DNA fingerprint fragments in recombinant inbred strains of mice has shown that murine minisatellites are not preferentially associated with centromers or telomeres, and it is likely that their human counterparts are similarly dispersed.
  • the tandem repetitive minisatellite probes which have been tested are very sensitive, presumably as a result of the repetitive nature of both the hybridization probe and the target minisatellite DNA.
  • the Southern blot autoradiographic signal obtained after overnight hybridization is saturated at probe DNA concentrations of >0.1 ng/ml (data not shown), and signals can be readily obtained from 60 ng or less of human genomic DNA (see FIG. 11A).
  • these probes can detect an admixture of 2% or less of one individual's DNA with another (see FIG. 11B).
  • FIG. 11C shows an analysis of semen stains from two victims who had been sexually assaulted and destroyed, where the recovery of DNA from the second victim was insufficient for conventional DNA fingerprint analysis which requires at least 0.5 ⁇ g DNA per test (see UK Patent Publication No. 2,166,445).
  • FIG. 11 shows an assessment of the sensitivity of locus specific probes and their application to the forensic analysis of a double rape/murder case.
  • FIG. 11A decreasing amounts of human DNA digested with HinfI were Southern blot hybridized with 32 P-labelled insert DNA from MS1. Filters were autoradiographed in the presence of an intensifier screen for 1 week.
  • FIG. 11B decreasing amounts of individual A DNA were mixed with 4 ⁇ g individual B DNA in the indicated ratios, and Southern blot hybridized, with ⁇ MS1 as in FIG. 11A.
  • victims X and Y had been sexually assaulted and destroyed.
  • DNA from the following forensic specimens was isolated and analysed: a, hair from X taken two days post-mortem; b, a mixture of semen and vaginal fluid recovered from the pubic hair of X; c, fresh blood from suspect Z; d, cardiac blood taken one day post-mortem from Y; e, vaginal swab from Y bearing semen, blood and vaginal material; F, a stain from Y's skirt bearing semen and blood.
  • Samples a and b had been stored dry at 4° for 3 years, samples d and e at 4° for 2 months and sample f stored dry at 4° for 2 months.
  • DNA was extracted following the procedures of Gill, Jeffreys and Werrett (1985) Nature 318, 577-579, digested with HinfI and Southern blot hybridized to 32 p-labelled ⁇ MS1 insert. 0.8 ⁇ g DNA was analyzed in each sample, except for sample e (0.1 ⁇ g DNA) and f (0.04 ⁇ g DNA). Autoradiography was for one week in the presence of an intensifier screen. Note that the semen stains b, e and f each contain two hybridizing DNA fragments which are not attributable to the victim.
  • sample b contains the victim's alleles derived from vaginal DNA.
  • the semen alleles in b, e and f are indistinguishable, suggesting that victims X and Y had indeed been sexually assaulted by the same man.
  • the alleles of suspect Z do not match those of the semen stains.
  • the multilocus Southern blot patterns are highly individual-specific with only 18% (S.D. ⁇ 11%) of DNA fragments shared between pairs of randomly-selected North Europeans (40 pairs tested). For sibs, the level of fragment sharing rises as expected to -57% (10 sib pairs tested).
  • each offspring contains 3.4 ⁇ 0.5 (S.D.) DNA fragments which are specifically of paternal origin, and an equal number of maternal-specific DNA fragments (10 father/mother/child trios tested).
  • FIG. 12 shows the detection of multiple hypervariable loci in human DNA by hybridisation with pooled minisatellite probes.
  • 4 ⁇ g DNA samples from a random selection of English people (1-6), from sib pairs (7, 8 and 9, 10) and from father/child/mother family groups (11, 12, 13 and 14, 15, 16) were digested with HinfI and electrophoresed through a 0.8% agarose gel until all fragments less than 2 kb had electrophoresed off the gel.
  • the digests were Southern blot hybridized with 2 ng of insert DNA from each p ⁇ g3, ⁇ MS1, ⁇ MS8, ⁇ MS31 and ⁇ MS43; inserts were pooled prior to labelling with 32 p by random oligonucleotide priming. Variations in band labelling intensity presumably arise from variability in the efficiency of labelling and hybridization of individual probes.

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