US3826364B1 - - Google Patents
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- Publication number
- US3826364B1 US3826364B1 US25544372A US3826364B1 US 3826364 B1 US3826364 B1 US 3826364B1 US 25544372 A US25544372 A US 25544372A US 3826364 B1 US3826364 B1 US 3826364B1
- Authority
- US
- United States
- Prior art keywords
- cells
- drops
- nozzle
- stream
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000002245 particle Substances 0.000 abstract 5
- 239000012530 fluid Substances 0.000 abstract 2
- 230000003111 delayed effect Effects 0.000 abstract 1
- 238000005286 illumination Methods 0.000 abstract 1
- 238000007689 inspection Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
- 230000001360 synchronised effect Effects 0.000 abstract 1
- 230000007704 transition Effects 0.000 abstract 1
Images
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/34—Sorting according to other particular properties
- B07C5/342—Sorting according to other particular properties according to optical properties, e.g. colour
- B07C5/3425—Sorting according to other particular properties according to optical properties, e.g. colour of granular material, e.g. ore particles, grain
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B07—SEPARATING SOLIDS FROM SOLIDS; SORTING
- B07C—POSTAL SORTING; SORTING INDIVIDUAL ARTICLES, OR BULK MATERIAL FIT TO BE SORTED PIECE-MEAL, e.g. BY PICKING
- B07C5/00—Sorting according to a characteristic or feature of the articles or material being sorted, e.g. by control effected by devices which detect or measure such characteristic or feature; Sorting by manually actuated devices, e.g. switches
- B07C5/36—Sorting apparatus characterised by the means used for distribution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1404—Handling flow, e.g. hydrodynamic focusing
- G01N2015/1406—Control of droplet point
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US00255443A US3826364A (en) | 1972-05-22 | 1972-05-22 | Particle sorting method and apparatus |
DE2246380A DE2246380C2 (de) | 1972-05-22 | 1972-09-21 | Vorrichtung zum Trennen bzw. Sortieren von Teilchen |
CA152,527A CA974193A (en) | 1972-05-22 | 1972-09-26 | Chopped stream fluidized particle sensor charger and deflector |
JP48044234A JPS49109093A (de) | 1972-05-22 | 1973-04-20 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US00255443A US3826364A (en) | 1972-05-22 | 1972-05-22 | Particle sorting method and apparatus |
Publications (2)
Publication Number | Publication Date |
---|---|
US3826364A US3826364A (en) | 1974-07-30 |
US3826364B1 true US3826364B1 (de) | 1984-09-25 |
Family
ID=22968350
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US00255443A Expired - Lifetime US3826364A (en) | 1972-05-22 | 1972-05-22 | Particle sorting method and apparatus |
Country Status (4)
Country | Link |
---|---|
US (1) | US3826364A (de) |
JP (1) | JPS49109093A (de) |
CA (1) | CA974193A (de) |
DE (1) | DE2246380C2 (de) |
Cited By (151)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3963606A (en) * | 1974-06-03 | 1976-06-15 | Coulter Electronics, Inc. | Semi-automatic adjusting delay for an electronic particle separator |
US3976197A (en) * | 1974-11-22 | 1976-08-24 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation method and apparatus for controlling the sex of mammalian offspring |
US4097373A (en) * | 1977-03-23 | 1978-06-27 | John Caldwell Allred | High speed particle sorter using a field emission electrode |
FR2397634A1 (fr) * | 1977-07-16 | 1979-02-09 | Deutsches Krebsforsch | Procede et dispositif d'analyse de la fluorescence de particules colorees, en particulier de cellules biologiques |
US4148718A (en) * | 1976-06-10 | 1979-04-10 | Coulter Electronics, Inc. | Single drop separator |
US4175662A (en) * | 1977-04-12 | 1979-11-27 | Tibor Zold | Method and device for sorting particles suspended in an electrolyte |
US4230558A (en) * | 1978-10-02 | 1980-10-28 | Coulter Electronics, Inc. | Single drop separator |
US4279345A (en) * | 1979-08-03 | 1981-07-21 | Allred John C | High speed particle sorter using a field emission electrode |
US4317520A (en) * | 1979-08-20 | 1982-03-02 | Ortho Diagnostics, Inc. | Servo system to control the spatial position of droplet formation of a fluid jet in a cell sorting apparatus |
US4318481A (en) * | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method for automatically setting the correct phase of the charge pulses in an electrostatic flow sorter |
US4318483A (en) * | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Automatic relative droplet charging time delay system for an electrostatic particle sorting system using a relatively moveable stream surface sensing system |
US4318480A (en) * | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method and apparatus for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting device |
US4318482A (en) * | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method for measuring the velocity of a perturbed jetting fluid in an electrostatic particle sorting system |
US4325483A (en) * | 1979-08-20 | 1982-04-20 | Ortho Diagnostics, Inc. | Method for detecting and controlling flow rates of the droplet forming stream of an electrostatic particle sorting apparatus |
US4347935A (en) * | 1979-05-16 | 1982-09-07 | The United States Of America As Represented By The United States Department Of Energy | Method and apparatus for electrostatically sorting biological cells |
US4367043A (en) * | 1980-05-05 | 1983-01-04 | Leland Stanford Junior University | Method and means for delivering liquid samples to a sample scanning device |
US4395397A (en) * | 1981-09-17 | 1983-07-26 | Sidney Farber Cancer Institute, Inc. | Apparatus and method for killing unwanted cells |
FR2543298A1 (fr) * | 1983-03-24 | 1984-09-28 | Coulter Corp | Dispositif d'analyse et de triage de particules |
US4487320A (en) * | 1980-11-03 | 1984-12-11 | Coulter Corporation | Method of and apparatus for detecting change in the breakoff point in a droplet generation system |
US4498766A (en) * | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
US4499052A (en) * | 1982-08-30 | 1985-02-12 | Becton, Dickinson And Company | Apparatus for distinguishing multiple subpopulations of cells |
FR2557977A1 (fr) * | 1984-01-06 | 1985-07-12 | Us Energy | Appareil pour eliminer une interference de bruit de fond dans des mesures de fluorescence |
US4538733A (en) * | 1983-10-14 | 1985-09-03 | Becton, Dickinson And Company | Particle sorter with neutralized collection wells and method of using same |
US4545677A (en) * | 1984-03-05 | 1985-10-08 | Becton, Dickinson And Company | Prismatic beam expander for light beam shaping in a flow cytometry apparatus |
US4599307A (en) * | 1983-07-18 | 1986-07-08 | Becton, Dickinson And Company | Method for elimination of selected cell populations in analytic cytology |
EP0210343A1 (de) * | 1985-07-29 | 1987-02-04 | Becton, Dickinson and Company | Durchfluss-Zytometriegerät mit verbesserter Lichtstrahljustierung |
US4663522A (en) * | 1984-10-05 | 1987-05-05 | Spandrel Establishment | Integrating sphere device for measuring transmission of light in objects |
US4667830A (en) * | 1981-06-15 | 1987-05-26 | The Board Of Trustees Of The Leland Stanford Junior University | Method and means for sorting individual particles into containers for culturing, cloning, analysis, or the like |
US4691829A (en) * | 1980-11-03 | 1987-09-08 | Coulter Corporation | Method of and apparatus for detecting change in the breakoff point in a droplet generation system |
US4701664A (en) * | 1986-01-09 | 1987-10-20 | Becton, Dickinson And Company | Mercury arc lamp suitable for inclusion in a flow cytometry apparatus |
US4710635A (en) * | 1986-04-14 | 1987-12-01 | Becton, Dickinson And Company | Dual laser excitation from single laser source |
US4745285A (en) * | 1986-08-21 | 1988-05-17 | Becton Dickinson And Company | Multi-color fluorescence analysis with single wavelength excitation |
US4781459A (en) * | 1985-05-17 | 1988-11-01 | Horiba, Ltd. | Apparatus for measuring the amount of minute particles contained in liquid |
WO1989000894A1 (en) * | 1987-08-05 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junio | Apparatus and method for multidimensional characterization of objects in real time |
WO1990013368A1 (en) * | 1989-05-01 | 1990-11-15 | Coulter Corporation | Coincidence arbitration in a flow cytometry sorting system |
US5047321A (en) * | 1988-06-15 | 1991-09-10 | Becton Dickinson & Co. | Method for analysis of cellular components of a fluid |
US5057413A (en) * | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
US5064616A (en) * | 1987-11-30 | 1991-11-12 | Becton Dickinson And Company | Kit for analysis of subsets of subpopulations of leukocytes |
EP0455867A2 (de) * | 1990-05-07 | 1991-11-13 | Esm International, Inc. | Sortiervorrichtung mit pünktlicher Photobehandlung |
EP0461618A2 (de) * | 1990-06-13 | 1991-12-18 | Becton, Dickinson and Company | Reinigungszyklus für Durchfluss-Zytometer |
US5076472A (en) * | 1990-06-13 | 1991-12-31 | Becton, Dickinson And Company | Cleaning cycle for flow cytometers |
US5089384A (en) * | 1988-11-04 | 1992-02-18 | Amoco Corporation | Method and apparatus for selective cell destruction using amplified immunofluorescence |
US5098849A (en) * | 1988-07-13 | 1992-03-24 | Becton Dickinson And Company | Material and method to reduce non-specific binding of a labelled material |
US5108904A (en) * | 1990-03-26 | 1992-04-28 | Alan Landay | CD44 as a marker for HIV infection |
US5123731A (en) * | 1988-02-01 | 1992-06-23 | Canon Kabushiki Kaisha | Particle measuring device |
US5156951A (en) * | 1989-07-13 | 1992-10-20 | Becton Dickinson And Company | Detecting immunological changes in HIV infected patient samples |
US5180065A (en) * | 1989-10-11 | 1993-01-19 | Canon Kabushiki Kaisha | Apparatus for and method of fractionating particle in particle-suspended liquid in conformity with the properties thereof |
US5199576A (en) * | 1991-04-05 | 1993-04-06 | University Of Rochester | System for flexibly sorting particles |
US5224058A (en) * | 1990-05-01 | 1993-06-29 | Becton, Dickinson And Company | Method for data transformation |
US5232828A (en) * | 1992-03-09 | 1993-08-03 | Becton, Dickinson And Company | Coating agents for cell recovery |
EP0553951A1 (de) * | 1992-01-30 | 1993-08-04 | Toa Medical Electronics Company, Limited | Teilchenbestimmungsgerät |
US5247340A (en) * | 1991-02-27 | 1993-09-21 | Toa Medical Electronics Co., Ltd. | Flow imaging cytometer |
US5247339A (en) * | 1991-02-27 | 1993-09-21 | Toa Medical Electronics Co., Ltd. | Flow imaging cytometer |
US5275787A (en) * | 1989-10-04 | 1994-01-04 | Canon Kabushiki Kaisha | Apparatus for separating or measuring particles to be examined in a sample fluid |
WO1996012171A2 (en) * | 1994-10-14 | 1996-04-25 | University Of Washington | High speed flow cytometer droplet formation system |
EP0711991A1 (de) * | 1994-11-14 | 1996-05-15 | TOA MEDICAL ELECTRONICS CO., Ltd. | Teilchenanalysegerät |
US5602039A (en) * | 1994-10-14 | 1997-02-11 | The University Of Washington | Flow cytometer jet monitor system |
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US5726364A (en) * | 1994-10-14 | 1998-03-10 | The University Of Washington | Sample introduction apparatus for a flow cytometer |
US5915925A (en) * | 1997-01-07 | 1999-06-29 | North, Jr.; Howard L. | Pulseless liquid supply system for flow cytometry |
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US6332540B1 (en) * | 1997-04-08 | 2001-12-25 | Smithkline Beecham P.L.C. | Device for isolating small polymeric beads from a suspension of such beads |
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WO2002072766A2 (en) | 2001-03-09 | 2002-09-19 | Board Of Regents, Universitx Of Texas System | Induction of tumor immunity by variants of folate binding protein |
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Also Published As
Publication number | Publication date |
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US3826364A (en) | 1974-07-30 |
DE2246380C2 (de) | 1982-11-04 |
CA974193A (en) | 1975-09-09 |
JPS49109093A (de) | 1974-10-17 |
DE2246380A1 (de) | 1973-12-20 |
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