FR2397634A1 - Procede et dispositif d'analyse de la fluorescence de particules colorees, en particulier de cellules biologiques - Google Patents

Procede et dispositif d'analyse de la fluorescence de particules colorees, en particulier de cellules biologiques

Info

Publication number
FR2397634A1
FR2397634A1 FR7821203A FR7821203A FR2397634A1 FR 2397634 A1 FR2397634 A1 FR 2397634A1 FR 7821203 A FR7821203 A FR 7821203A FR 7821203 A FR7821203 A FR 7821203A FR 2397634 A1 FR2397634 A1 FR 2397634A1
Authority
FR
France
Prior art keywords
fluorescence
analysis
biological cells
colored particles
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
FR7821203A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deutsches Krebsforschungszentrum DKFZ
Original Assignee
Deutsches Krebsforschungszentrum DKFZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Krebsforschungszentrum DKFZ filed Critical Deutsches Krebsforschungszentrum DKFZ
Publication of FR2397634A1 publication Critical patent/FR2397634A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1425—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement
    • G01N15/1427—Optical investigation techniques, e.g. flow cytometry using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1438—Using two lasers in succession

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

a. Procédé d'analyse par fluorescence de particules à colorations multiples, en particulier de cellules biologiques. b. Procedé amélioré sans limitation du choix et de la combinaison des colorants appropriés. c. Recherche de cellules cancéreuses.
FR7821203A 1977-07-16 1978-07-17 Procede et dispositif d'analyse de la fluorescence de particules colorees, en particulier de cellules biologiques Pending FR2397634A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2732272A DE2732272C2 (de) 1977-07-16 1977-07-16 Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefärbten Partikeln, insbesondere biologischen Zellen

Publications (1)

Publication Number Publication Date
FR2397634A1 true FR2397634A1 (fr) 1979-02-09

Family

ID=6014129

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7821203A Pending FR2397634A1 (fr) 1977-07-16 1978-07-17 Procede et dispositif d'analyse de la fluorescence de particules colorees, en particulier de cellules biologiques

Country Status (4)

Country Link
US (1) US4243318A (fr)
DE (1) DE2732272C2 (fr)
FR (1) FR2397634A1 (fr)
GB (1) GB2002109B (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2537723A1 (fr) * 1982-12-14 1984-06-15 Packard Instrument Co Inc Procede et composition pour evaluer la reaction phagocytaire

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SE8102772L (sv) * 1981-05-04 1982-11-05 Sven Staffan Folestad Forfarande for laserinducerad fluoriscensdetektering och anordning for genomforande av forfarandet
DE3208919A1 (de) * 1982-03-12 1983-09-22 Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München Anordnung zur messung der fluoreszenzpolarisation
US4498766A (en) * 1982-03-25 1985-02-12 Becton, Dickinson And Company Light beam focal spot elongation in flow cytometry devices
US4717655A (en) * 1982-08-30 1988-01-05 Becton, Dickinson And Company Method and apparatus for distinguishing multiple subpopulations of cells
US4581334A (en) * 1983-04-25 1986-04-08 Ortho Diagnostics Systems, Inc. Simultaneous detection of leukocyte phagocytic and killing ability
US4599307A (en) * 1983-07-18 1986-07-08 Becton, Dickinson And Company Method for elimination of selected cell populations in analytic cytology
US4573796A (en) * 1984-01-06 1986-03-04 The United States Of America As Represented By The United States Department Of Energy Apparatus for eliminating background interference in fluorescence measurements
US4545677A (en) * 1984-03-05 1985-10-08 Becton, Dickinson And Company Prismatic beam expander for light beam shaping in a flow cytometry apparatus
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
JPS61270639A (ja) * 1985-05-25 1986-11-29 Japan Spectroscopic Co フロ−サイトメ−タ
JPS6214285A (ja) * 1985-07-12 1987-01-22 Dan Kagaku:Kk 自動浮遊粒子計数器
US4989977A (en) * 1985-07-29 1991-02-05 Becton, Dickinson And Company Flow cytometry apparatus with improved light beam adjustment
JPS62123830A (ja) * 1985-11-25 1987-06-05 Sharp Corp ロ−タリ−エンコ−ダ
JPS62126326A (ja) * 1985-11-26 1987-06-08 Rion Co Ltd 粒子計測方法
US5123731A (en) * 1988-02-01 1992-06-23 Canon Kabushiki Kaisha Particle measuring device
US5760900A (en) * 1989-03-18 1998-06-02 Canon Kabushiki Kaisha Method and apparatus for optically measuring specimen
US5275787A (en) * 1989-10-04 1994-01-04 Canon Kabushiki Kaisha Apparatus for separating or measuring particles to be examined in a sample fluid
DE69028687T2 (de) * 1989-12-15 1997-04-10 Canon Kk Vorrichtung zur optischen Messung einer Probe
DE69118429T2 (de) * 1990-01-26 1996-09-12 Canon Kk Verfahren zur Messung einer Spezies unter Verwendung von Fluoreszenzlicht
US5093866A (en) * 1990-02-09 1992-03-03 Hamilton Equine Associates Limited Fluorescence and motility characterization system for cells, bacteria, and particles in fluids
US5307148A (en) * 1990-04-05 1994-04-26 Hitachi, Ltd. Fluorescence detection type electrophoresis apparatus
JP3121849B2 (ja) * 1991-02-27 2001-01-09 シスメックス株式会社 フローイメージサイトメータ
JP3084296B2 (ja) * 1991-02-27 2000-09-04 シスメックス株式会社 フローイメージサイトメータ
US5293213A (en) * 1992-08-12 1994-03-08 Klein Uwe K A Utilization of a modulated laser beam in heterodyne interferometry
US5793485A (en) * 1995-03-20 1998-08-11 Sandia Corporation Resonant-cavity apparatus for cytometry or particle analysis
US5608519A (en) * 1995-03-20 1997-03-04 Gourley; Paul L. Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells
US5528045A (en) * 1995-04-06 1996-06-18 Becton Dickinson And Company Particle analyzer with spatially split wavelength filter
US6731100B1 (en) 1997-05-05 2004-05-04 Chemometec A/S Method and a system for determination of somatic cells in milk
EP1935983B1 (fr) * 1997-05-05 2011-06-22 ChemoMetec A/S Procédé de détermination des particules biologiques dans le sang
US6710871B1 (en) 1997-06-09 2004-03-23 Guava Technologies, Inc. Method and apparatus for detecting microparticles in fluid samples
WO1998057152A1 (fr) 1997-06-09 1998-12-17 Guava Technologies, Inc. Procede et dispositif pour deceler des microparticules dans des echantillons de fluides
JPH11295208A (ja) * 1998-04-13 1999-10-29 Sysmex Corp 粒子撮像装置
US6320196B1 (en) 1999-01-28 2001-11-20 Agilent Technologies, Inc. Multichannel high dynamic range scanner
US6628385B1 (en) 1999-02-05 2003-09-30 Axon Instruments, Inc. High efficiency, large field scanning microscope
US6549275B1 (en) * 2000-08-02 2003-04-15 Honeywell International Inc. Optical detection system for flow cytometry
JP4266075B2 (ja) * 2001-02-19 2009-05-20 株式会社堀場製作所 粒径分布測定装置
CA2463420A1 (fr) * 2001-10-24 2003-05-01 Singulex, Inc. Procedes destines a detecter des haplotypes genetiques par interaction avec des sondes
AU2003298672A1 (en) * 2002-11-19 2005-01-28 Singulex, Inc. Detection of target molecules through interaction with probes
WO2005019419A2 (fr) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection de molecules individuelles de polypeptides et de polynucleotides
US7796173B2 (en) * 2003-08-13 2010-09-14 Lettvin Jonathan D Imaging system
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
WO2005050392A2 (fr) * 2003-11-17 2005-06-02 Lettvin Jonathan D Remappage geometrique au moyen de lignes de temporisation
US7440101B2 (en) * 2004-01-23 2008-10-21 Beckman Coulter, Inc. System and method for multiple laser triggering
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
EP1805500A4 (fr) * 2004-09-28 2008-05-07 Singulex Inc Systeme et methode d'analyse d'echantillons
CA3086149A1 (fr) 2006-04-04 2007-10-11 Singulex, Inc. Systeme et procedes hautement sensibles destines a une analyse de la troponine
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
WO2009073649A1 (fr) 2007-12-04 2009-06-11 Particle Measuring Systems, Inc. Systèmes et procédés de détection de particules non orthogonaux
CN103543094B (zh) 2007-12-19 2017-06-09 神谷来克斯公司 单分子检测用扫描分析器和使用方法
AU2009234106A1 (en) * 2008-03-05 2009-10-15 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
GB2464183A (en) * 2008-09-19 2010-04-14 Singulex Inc Sandwich assay
AU2010259022B2 (en) 2009-06-08 2016-05-12 Singulex, Inc. Highly sensitive biomarker panels
US20110301053A1 (en) 2010-05-06 2011-12-08 Singulex, Inc. Methods for Diagnosing, Staging, Predicting Risk for Developing and Identifying Treatment Responders for Rheumatoid Arthritis
US20210341380A1 (en) * 2020-05-04 2021-11-04 Becton, Dickinson And Company Flow cytometers including laser assessors, and methods for using the same

Citations (3)

* Cited by examiner, † Cited by third party
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US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US3916197A (en) * 1973-11-28 1975-10-28 Particle Technology Inc Method and apparatus for classifying biological cells
US3960449A (en) * 1975-06-05 1976-06-01 The Board Of Trustees Of Leland Stanford Junior University Measurement of angular dependence of scattered light in a flowing stream

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US3976862A (en) * 1975-03-18 1976-08-24 Block Engineering, Inc. Flow stream processor

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US3826364B1 (fr) * 1972-05-22 1984-09-25
US3916197A (en) * 1973-11-28 1975-10-28 Particle Technology Inc Method and apparatus for classifying biological cells
US3960449A (en) * 1975-06-05 1976-06-01 The Board Of Trustees Of Leland Stanford Junior University Measurement of angular dependence of scattered light in a flowing stream

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
EXBK/75 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2537723A1 (fr) * 1982-12-14 1984-06-15 Packard Instrument Co Inc Procede et composition pour evaluer la reaction phagocytaire

Also Published As

Publication number Publication date
DE2732272B1 (de) 1978-10-26
DE2732272C2 (de) 1979-07-05
US4243318A (en) 1981-01-06
GB2002109B (en) 1982-01-13
GB2002109A (en) 1979-02-14

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