WO2005033283A3 - Methods for enhancing the analysis of particle detection - Google Patents

Methods for enhancing the analysis of particle detection Download PDF

Info

Publication number
WO2005033283A3
WO2005033283A3 PCT/US2004/032244 US2004032244W WO2005033283A3 WO 2005033283 A3 WO2005033283 A3 WO 2005033283A3 US 2004032244 W US2004032244 W US 2004032244W WO 2005033283 A3 WO2005033283 A3 WO 2005033283A3
Authority
WO
WIPO (PCT)
Prior art keywords
enhancing
analysis
methods
particle
particle detection
Prior art date
Application number
PCT/US2004/032244
Other languages
French (fr)
Other versions
WO2005033283A2 (en
Inventor
Robert S Puskas
Original Assignee
Robert S Puskas
Singulex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US50724303P priority Critical
Priority to US60/507,243 priority
Application filed by Robert S Puskas, Singulex Inc filed Critical Robert S Puskas
Priority claimed from US10/574,055 external-priority patent/US20080021674A1/en
Publication of WO2005033283A2 publication Critical patent/WO2005033283A2/en
Publication of WO2005033283A3 publication Critical patent/WO2005033283A3/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1429Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1425Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its control arrangement
    • G01N15/1427Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its control arrangement with the synchronisation of components, a time gate for operation of components, or suppression of particle coincidences
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1463Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals using image analysis for extracting features of the particle
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0092Monitoring flocculation or agglomeration
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1402Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Electro-optical investigation, e.g. flow cytometers
    • G01N15/1434Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
    • G01N2015/1438Using two lasers in succession

Abstract

Methods for enhancing the analysis of particle detection are provided comprising measuring a first electromagnetic radiation signal provided by a particle, comparing by cross-correlation the electromagnetic radiation signal emitted by the particle, and further applying an analytical filter to the cross-correlation events, thereby enhancing the analysis of the particle emission.
PCT/US2004/032244 2003-09-30 2004-09-30 Methods for enhancing the analysis of particle detection WO2005033283A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US50724303P true 2003-09-30 2003-09-30
US60/507,243 2003-09-30

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20040789395 EP1676122A2 (en) 2003-09-30 2004-09-30 Methods for enhancing the analysis of particle detection
US10/574,055 US20080021674A1 (en) 2003-09-30 2004-09-30 Methods for Enhancing the Analysis of Particle Detection
JP2006534113A JP2007533971A (en) 2003-09-30 2004-09-30 How to improve the accuracy of particle detection

Publications (2)

Publication Number Publication Date
WO2005033283A2 WO2005033283A2 (en) 2005-04-14
WO2005033283A3 true WO2005033283A3 (en) 2005-08-04

Family

ID=34421599

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/032244 WO2005033283A2 (en) 2003-09-30 2004-09-30 Methods for enhancing the analysis of particle detection

Country Status (3)

Country Link
EP (1) EP1676122A2 (en)
JP (1) JP2007533971A (en)
WO (1) WO2005033283A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8917392B2 (en) 2007-12-19 2014-12-23 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US9182405B2 (en) 2006-04-04 2015-11-10 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US9494598B2 (en) 2006-04-04 2016-11-15 Singulex, Inc. Highly sensitive system and method for analysis of troponin

Families Citing this family (25)

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Publication number Priority date Publication date Assignee Title
US9040305B2 (en) 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US7333197B2 (en) * 2004-11-17 2008-02-19 Honeywell International Inc. Raman detection based flow cytometer
US9452429B2 (en) * 2006-02-02 2016-09-27 E. I. Spectra, Llc Method for mutiplexed microfluidic bead-based immunoassay
US8460878B2 (en) 2006-02-21 2013-06-11 The Trustees Of Tufts College Methods and arrays for detecting cells and cellular components in small defined volumes
JP6325022B2 (en) * 2006-04-04 2018-05-16 シングレックス,インコーポレイテッド Sensitive system and method for the analysis of troponin
JP5388846B2 (en) 2006-06-27 2014-01-15 バイオヴィジラント システムズ インコーポレイテッド Pathogen detection by simultaneous measurement of particle size and fluorescence
CA2734029C (en) 2007-08-30 2016-03-29 The Trustees Of Tufts College Methods for determining the concentration of an analyte in solution
WO2009057525A1 (en) * 2007-10-29 2009-05-07 Sysmex Corporation Cell analysis apparatus and cell analysis method
WO2009108223A2 (en) * 2007-11-09 2009-09-03 Biovigilant Systems, Inc. Pathogen detection by simultaneous size/fluorescence measurement
CN110296959A (en) 2008-06-10 2019-10-01 爱克斯崔里斯科技有限公司 Detection of particles
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
US8394251B2 (en) * 2008-10-07 2013-03-12 The Board Of Trustees Of The Leland Stanford Junior University Control of chemical reactions using isotachophoresis
KR101735576B1 (en) 2009-05-01 2017-05-15 엑스트랄리스 테크놀로지 리미티드 Improvements to Particle Detectors
AU2010259022B2 (en) 2009-06-08 2016-05-12 Singulex, Inc. Highly sensitive biomarker panels
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
WO2011109379A1 (en) 2010-03-01 2011-09-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US20140302532A1 (en) 2011-04-12 2014-10-09 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patient's recovery from a brain injury
JP6158835B2 (en) * 2012-01-11 2017-07-05 バクスアルタ ゲーエムベーハー Characterization of subvisible particles using a particle analyzer
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
WO2014141994A1 (en) * 2013-03-15 2014-09-18 国立大学法人 東京大学 Particle analyzing method and particle analyzing device
SG11201806190TA (en) 2016-01-29 2018-08-30 Purigen Biosystems Inc Isotachophoresis for purification of nucleic acids

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5528045A (en) * 1995-04-06 1996-06-18 Becton Dickinson And Company Particle analyzer with spatially split wavelength filter

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DE3208919C2 (en) * 1982-03-12 1992-03-26 Gsf - Forschungszentrum Fuer Umwelt Und Gesundheit, Gmbh, 8000 Muenchen, De
JPH03128434A (en) * 1989-10-13 1991-05-31 Canon Inc Particle measuring instrument
JPH04188045A (en) * 1990-11-21 1992-07-06 Canon Inc Specimen measuring apparatus
JPH0792076A (en) * 1993-09-24 1995-04-07 Canon Inc Grain analyzing device
JPH0792077A (en) * 1993-09-24 1995-04-07 Canon Inc Grain analyzing device
JPH11258144A (en) * 1998-03-13 1999-09-24 Sysmex Corp Particle image-analyzing apparatus
US20030124592A1 (en) * 2001-10-24 2003-07-03 Bioprofile, Llc Methods for detecting genetic haplotypes by interaction with probes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US3826364B1 (en) * 1972-05-22 1984-09-25
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
US5528045A (en) * 1995-04-06 1996-06-18 Becton Dickinson And Company Particle analyzer with spatially split wavelength filter

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9182405B2 (en) 2006-04-04 2015-11-10 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US9494598B2 (en) 2006-04-04 2016-11-15 Singulex, Inc. Highly sensitive system and method for analysis of troponin
US8917392B2 (en) 2007-12-19 2014-12-23 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
US9239284B2 (en) 2007-12-19 2016-01-19 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use

Also Published As

Publication number Publication date
EP1676122A2 (en) 2006-07-05
JP2007533971A (en) 2007-11-22
WO2005033283A2 (en) 2005-04-14

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