US20170016000A1 - Compositions and agents against hepatitis b virus and uses thereof - Google Patents

Compositions and agents against hepatitis b virus and uses thereof Download PDF

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US20170016000A1
US20170016000A1 US15/212,279 US201615212279A US2017016000A1 US 20170016000 A1 US20170016000 A1 US 20170016000A1 US 201615212279 A US201615212279 A US 201615212279A US 2017016000 A1 US2017016000 A1 US 2017016000A1
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compound
seq
monomers
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Pattraranee Limphong
Kiyoshi Tachikawa
Christine Esau
Padmanabh Chivukula
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Arcturus Therapeutics Inc
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Arcturus Therapeutics Inc
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Priority to US15/212,279 priority Critical patent/US20170016000A1/en
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Priority to US15/410,984 priority patent/US20170137821A1/en
Priority to US16/117,994 priority patent/US10961535B2/en
Priority to US16/724,122 priority patent/US11685921B2/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/34—Spatial arrangement of the modifications
    • C12N2310/344—Position-specific modifications, e.g. on every purine, at the 3'-end

Definitions

  • Hepatitis B is a liver disease that results from infection with the Hepatitis B virus (HBV). Its severity can be from a mild illness lasting a few weeks, to a serious, lifelong illness. Hepatitis B can be either acute or chronic. Acute Hepatitis B virus infection is a short-term illness that may lead to chronic infection. Chronic Hepatitis B virus infection is a long-term illness that can result in long-term health problems, such as cirrhosis of the liver, liver cancer, and death.
  • HBV Hepatitis B virus
  • Hepatitis B is usually spread through transfer of a body fluid by sexual contact with an infected person, or through sharing needles for drug-injection. It can also be passed from an infected mother to her baby at birth. In endemic areas, Hepatitis B is most often spread from mother to child at birth, or by exposure to infected blood, especially from an infected child to an uninfected child during the first 5 years of life.
  • Hepatitis B defined as Hepatitis B surface antigen positive for at least 6 months. Approximately 780,000 persons die each year from Hepatitis B infection.
  • hepatitis B infection can be done by detecting the hepatitis B surface antigen HBsAg.
  • Acute hepatitis B virus infection is characterized by the presence of HBsAg and immunoglobulin M (IgM) antibody to the core antigen, HBcAg.
  • IgM immunoglobulin M
  • HBeAg is usually a marker of high levels of replication of the virus. The presence of HBeAg indicates that the blood and body fluids of the infected individual are highly contagious.
  • Chronic infection is characterized by the persistence of HBsAg for at least 6 months, with or without concurrent HBeAg. Persistence of HBsAg is the principal marker of risk for developing chronic liver disease and liver cancer later in life.
  • HBV is a member of the hepadnavirus family.
  • the virus particles which can infect liver cells, are 30-42 nm in diameter and have an outer envelope and an icosahedral nucleocapsid core.
  • the nucleocapsid encloses the viral DNA, and a DNA polymerase that can have reverse transcriptase activity.
  • the outer envelope contains proteins that can be involved in viral binding and entry into cells.
  • HBV has four identified genes, C, P, S, and X.
  • Gene C codes for a core protein, HBcAg.
  • An extracellular protein HBeAg is processed from a pre-core protein.
  • a DNA polymerase is encoded by gene P.
  • Gene S codes for the small surface antigen HBsAg, which is one of three polypeptide surface proteins: large, middle, and small.
  • Gene X may be associated with development of liver cancer.
  • HBV is a pararetrovirus, which is a non-retrovirus that uses reverse transcription in the replication process.
  • the virus can enter the cell and multiply using RNA made by a host process.
  • the viral genomic DNA can be transferred to the cell nucleus, acted upon by viral polymerase, and provide transcription of four viral mRNAs by host RNA polymerase.
  • a large viral mRNA is used to make the new copies of the genome by reverse transcription, and to make the core protein and the viral DNA polymerase.
  • the viral mRNAs are further processed to form new virus particles.
  • HBV can be described by four major serotypes based on epitopes presented by envelope proteins: adr, adw, ayr, ayw. HBV has been identified with eight genotypes, A-H, as well as subgenotypes. The genotypes can have distinct geographical distribution, and are used in tracking evolution and transmission of the virus.
  • compositions and methods for treatment of Hepatitis B are compositions and methods for treatment of Hepatitis B.
  • This invention relates to the fields of biopharmaceuticals and therapeutics composed of oligomers for gene silencing. More particularly, this invention relates to structures, compositions and methods for therapeutic oligomers directed against Hepatitis B virus.
  • This invention provides novel molecules to be used as therapeutic agents against Hepatitis B infection.
  • the molecules of this invention can be used as active pharmaceutical ingredients in compositions for ameliorating, preventing or treating Hepatitis B infection.
  • Molecules of this invention for treating Hepatitis B infection may act against any of the replication, maturation, growth, or transmission modalities of the Hepatitis B virus. By preventing the Hepatitis B virus from carrying out any one or more of its processes, the molecules of this invention can be used for ameliorating or treating Hepatitis B infection.
  • Embodiments of this invention can provide molecules having one or more properties that advantageously provide enhanced effectiveness against HBV, as well as compositions or formulations for therapeutic agents against Hepatitis B infection, which can provide clinical agents.
  • the properties of the molecules of this invention arise according to their structure, and the molecular structure in its entirety, as a whole, can provide significant benefits and properties.
  • the active agents of this invention include oligomeric molecules that can inhibit expression of an HBV genome. Oligomers of this invention can provide potent action against HBV infection in a subject by silencing expression of an HBV genome.
  • a wide range of novel molecules are provided, which can incorporate one or more linker groups.
  • the linker groups can be attached in a chain in the molecule.
  • Each linker group can also be attached to a nucleobase.
  • a linker group can be a monomer. Monomers can be attached to form a chain molecule. In a chain molecule of this invention, a linker group monomer can be attached at any point in the chain.
  • linker group monomers can be attached in a chain molecule of this invention so that the linker group monomers reside near the ends of the chain.
  • the ends of the chain molecule can be formed by linker group monomers.
  • the linker groups of a chain molecule can each be attached to a nucleobase.
  • the presence of nucleobases in the chain molecule can provide a sequence of nucleobases.
  • this invention provides oligomer molecules having chain structures that incorporate novel combinations of the linker group monomers, along with certain natural nucleotides, or non-natural nucleotides, or modified nucleotides, or chemically-modified nucleotides.
  • the oligomer molecules of this invention can display a sequence of nucleobases that is targeted to a component of the HBV genome.
  • this invention provides therapeutics for preventing, ameliorating, or treating a disease caused by Hepatitis B infection.
  • An active compound or molecule of this invention may be used in the prevention or treatment of a viral infection caused by Hepatitis B virus.
  • This invention provides structures, methods and compositions for oligomeric agents that incorporate the linker group monomers.
  • the oligomeric molecules of this invention can be used as active agents in formulations for gene silencing therapeutics targeted to HBV.
  • a compound comprising a first strand and a second strand, each of the strands being 19-29 monomers in length, the monomers comprising UNA monomers and nucleic acid monomers, wherein the compound has a duplex region of from 14 to 29 contiguous monomers in length, wherein the first strand is a passenger strand for RNA interference and the second strand is a guide strand for RNA interference, and wherein the compound comprises a sequence of bases targeted to inhibit expression of an HBV genome.
  • the compound may contain from one to seven UNA monomers.
  • the compound may contain a UNA monomer at the 1-end (5′ end for non-UNA) of the first strand, a UNA monomer at the 3-end (3′ end for non-UNA) of the first strand, and a UNA monomer at the second position from the 5′ end of the second strand.
  • a compound can contain a UNA monomer at any one or more of positions 2 to 8 from the 5′ end of the second strand.
  • a compound may have a 3′ overhang with one or more UNA monomers, natural nucleotides, non-natural nucleotides, modified nucleotides, or chemically-modified nucleotides, or combinations thereof.
  • the 3′ overhang can have one or more deoxythymidine nucleotides, 2′-O-methyl nucleotides, inverted abasic monomers, inverted thymidine monomers, L-thymidine monomers, or glyceryl nucleotides.
  • a compound may have one or more nucleic acid monomers that is a non-natural nucleotide, a modified nucleotide, or a chemically-modified nucleotide.
  • a compound may have one or more monomers connected by a phosphorothioate, a chiral phosphorothioate, or a phosphorodithioate linkage.
  • a compound may be conjugated to a delivery moiety, such as, for example, a moiety that binds to a glycoprotein receptor, a galactose, a galactosamine, a N-acetylgalactosamine, a GalNAc group, or a cholesterol delivery moiety.
  • a compound may be conjugated to a delivery moiety and have increased uptake in the liver as compared to an unconjugated compound.
  • This invention includes lipid nanoparticle-oligomer compounds, in which one or more compounds are attached to a lipid nanoparticle.
  • a composition of this disclosure can include one or more compounds and a pharmaceutically acceptable carrier.
  • the carrier may be lipid nanoparticles or liposomes.
  • a composition of this disclosure may contain a first compound targeted to a conserved region of HBV transcripts for genes X, C, P and S, a second compound targeted to inhibit HBsAg, a third compound targeted to a conserved region of HBV transcripts for genes X, C and S, and a pharmaceutically acceptable carrier.
  • Embodiments of this invention include compositions containing one or more compounds having reference positions from any of positions 1525 to 1582, 374 to 414, 1776 to 1782, 244 to 256, and 1818 to 1866.
  • a composition may include a compound having a reference position from 1525 to 1582, a compound having a reference position from 374 to 414, and a compound having a reference position from 1776 to 1782.
  • Embodiments of this invention further contemplate methods for preventing, ameliorating or treating a disease or condition associated with HBV infection in a subject in need, by administering to the subject an effective amount of a composition above.
  • the administration of the composition can reduce HBV viral titer in the subject.
  • a subject may have been diagnosed with a disease associated with Hepatitis B virus infection, for example, a liver disease.
  • This invention includes methods for inhibiting the replication, maturation, growth, or transmission of a Hepatitis B virus in a subject in need, by administering to the subject an effective amount of a composition above.
  • the composition may reduce serum concentration of HBsAg in the subject.
  • the administration of the composition may reduce serum concentration of HBsAg in the subject by 2-log 10 -fold, or by 2-log 10 -fold for at least 7 days.
  • the administration of the composition can reduce HBeAg in the subject, or HBV DNA in the subject.
  • This invention also contemplates methods for inhibiting expression of a Hepatitis B virus polynucleotide in a subject in need, by administering to the subject a composition above, as well as the use of a composition above for preventing, ameliorating or treating a disease or condition associated with Hepatitis B infection in a subject in need.
  • compositions for use in medical therapy, or for use in the treatment of the human or animal body includes the use of a composition for preparing or manufacturing a medicament for preventing, ameliorating or treating a disease or condition associated with Hepatitis B infection in a subject in need.
  • FIG. 1 shows a map of HBV protein coding regions and selected transcripts for a reference genome. Nucleotide position 1/3221 is designated at the top. Further designations are as follows: pre-S1, large HBsAg; pre-S2, medium HBsAg; S, HBsAg; P, polymerase; X, HBx protein; pre-C, pre-core/HBeAg; C, HB core Ag.
  • pre-S1 large HBsAg
  • pre-S2 medium HBsAg
  • S HBsAg
  • P polymerase
  • X HBx protein
  • pre-C pre-core/HBeAg
  • C HB core Ag.
  • the 2.4 kb, 2.1 kb, and 0.7 kb transcripts coding for the pre-S1/pre-S2/S, as well as the transcript coding the X protein are shown.
  • the pre-Core/HBeAg protein is generated from a long, 3.5 kb transcript (not shown) originating at position ⁇ 1700, while the core and polymerase proteins and the pre-genomic RNA used as a template for viral replication are generated from a ⁇ 200 nt shorter transcript.
  • the ranges of reference positions for certain UNA oligomers, designated UNA oligomer 1, UNA oligomer 2, and UNA oligomer 3, are shown.
  • FIG. 2 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulations, ⁇ 1 and -2, and an ascending dose was used.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice containing human hepatocytes (70%).
  • Treatment with both UNA oligomer 1576 and a UNA oligomer triad composition (1576, 380, 177) caused a rapid and sustained reduction in viral endpoint serum HBsAg.
  • FIG. 3 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • the dose-dependent response in vivo shows a pharmacological effect of the UNA oligomer composition.
  • the study used an ascending dose in which mice were administered every 4 days, up to day 40, and viral endpoints were monitored every 4 days through day 44.
  • FIG. 4 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • the study used an ascending dose in which mice were administered every 4 days, up to day 40, and viral endpoints were monitored every 4 days through day 44.
  • FIG. 5 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • the dose-dependent response in vivo shows a pharmacological effect of the UNA oligomer composition.
  • the study used an ascending dose in which mice were administered every 4 days, up to day 40, and viral endpoints were monitored every 4 days through day 44.
  • FIG. 6 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • FIG. 7 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • FIG. 8 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection. Treatment with UNA oligomers 1777, 380 and 1578 caused a rapid and sustained reduction in viral endpoint serum HBV DNA.
  • FIG. 9 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • Treatment with a UNA oligomer triad composition (1578, 380, 1777) caused a rapid and sustained reduction in viral endpoint serum HBsAg.
  • the dose-dependent response in vivo shows a pharmacological effect of the UNA oligomer composition.
  • FIG. 10 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • Treatment with a UNA oligomer triad composition (1578, 380, 1777) caused a rapid and sustained reduction in viral endpoint serum HBeAg.
  • the dose-dependent response in vivo shows a pharmacological effect of the UNA oligomer composition.
  • FIG. 11 shows HBV inhibitory effect in vivo for UNA oligomers, observed in a humanized PXB Mouse model of HBV infection.
  • Treatment with a UNA oligomer triad composition (1578, 380, 1777) caused a rapid and sustained reduction in viral endpoint serum HBV DNA.
  • the dose-dependent response in vivo shows a pharmacological effect of the UNA oligomer composition.
  • FIG. 12 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the AAV-HBV mouse model is a robust model for investigating HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • the study used an ascending dose, and serum viral endpoints were monitored 15 days before, and at least 22 days after treatment. Treatment with each of UNA oligomers 380, 1777, and 1576 caused a rapid and sustained reduction in viral endpoint serum HBsAg.
  • FIG. 13 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • Treatment with each of UNA oligomers 380, 1777, and 1576, as well as the UNA oligomer triad composition of the same compounds (1576, 380, 1777) caused a rapid and sustained reduction in viral endpoint serum HBeAg.
  • This head-to-head comparison shows that the triad composition provided surprisingly increased potency throughout the duration of the effect, relative to the individual oligomers.
  • FIG. 14 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • Treatment with each of UNA oligomers 380, 1777, and 1576 caused a rapid and sustained reduction in viral endpoint serum HBV DNA.
  • FIG. 15 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • the study was an ascending dose design in which mice were treated with 3 mg/kg on day 0, then 5 mg/kg on day 4, then 10 mg/kg on day 8, and serum viral endpoints were monitored up to day 12 after treatment.
  • Treatment with the UNA oligomer triad composition (1777, 380, 1578) caused a rapid and sustained reduction in viral endpoint serum HBsAg.
  • the dose-dependent response in vivo shows a pharmacological effect of the UNA oligomer composition.
  • FIG. 16 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • Treatment with each of UNA oligomers 1578 and 1575 caused a rapid and sustained reduction in viral endpoint serum HBsAg.
  • FIG. 17 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • Treatment with each of UNA oligomers 1578 and 1575 caused a rapid and sustained reduction in viral endpoint serum HBeAg.
  • FIG. 18 shows HBV inhibitory effect in vivo for UNA oligomers, observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers were formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • Treatment with each of UNA oligomers 1578 and 1575 caused a rapid and sustained reduction in viral endpoint serum HBV DNA.
  • This invention provides a range of novel agents and compositions to be used as therapeutics against Hepatitis B infection.
  • Molecules of this invention can be used as active pharmaceutical ingredients in compositions for ameliorating, preventing or treating Hepatitis B infection.
  • the galenic molecules of this invention can prevent Hepatitis B virus from carrying out one or more of its processes.
  • Molecules of this invention can be used for ameliorating or treating Hepatitis B infection, and may act against any of the replication, maturation, growth, or transmission modes of the Hepatitis B virus.
  • Novel agents of this invention include oligomeric molecules that inhibit expression of an HBV genome.
  • Embodiments of this invention can provide extraordinary and surprisingly enhanced efficacy against HBV infection in a subject by attacking all portions of the HBV genome. More particularly, agents and compositions of this invention can simultaneously inhibit all identified genes of HBV: C, P, S, and X. Thus, the compounds and compositions of this disclosure can inhibit the small surface antigen HBsAg, as well as the extracellular protein HBeAg, in addition to X protein and viral polymerase.
  • compositions or formulations for therapeutic agents against Hepatitis B infection which can provide clinical agents.
  • linker groups can be attached in a chain in the molecule.
  • linker group can also be attached to a nucleobase.
  • a linker group can be a monomer. Monomers can be attached to form a chain molecule. In a chain molecule of this invention, a linker group monomer can be attached at any point in the chain.
  • linker group monomers can be attached in a chain molecule of this invention so that the linker group monomers reside near the ends of the chain.
  • the ends of the chain molecule can be formed by linker group monomers.
  • a chain molecule can also be referred to as an oligomer.
  • the linker groups of a chain molecule can each be attached to a nucleobase.
  • the presence of nucleobases in the chain molecule can provide a sequence of nucleobases.
  • this invention provides oligomer molecules having chain structures that incorporate novel combinations of the linker group monomers, along with certain natural nucleotides, or non-natural nucleotides, or modified nucleotides, or chemically-modified nucleotides.
  • the oligomer molecules of this invention can display a sequence of nucleobases that is targeted to a component of an HBV genome.
  • an oligomer can be targeted to a portion of the HBV genome that is conserved, or highly conserved, among a number of known HBV genomic sequences.
  • this invention provides active oligomer molecules that correspond to, or are complementary to at least a fragment of an HBV nucleic acid molecule, and that decrease expression of at least such a fragment present in a cell.
  • the active oligomer molecule can be double-stranded.
  • a cellular pathway may use active oligomers of this invention to be sequence-specific regulators in an RNA interference pathway.
  • the active oligomers may bind to the RNA-induced silencing complex (RISC complex), where a sense strand, also referred to as the passenger strand, and an antisense strand, also referred to as the guide strand, can be unwound, and the antisense strand complexed in the RISC complex.
  • the guide strand can bind to a complementary sequence to which it was targeted, for example, a target sequence in an mRNA, which can be subsequently cleaved, resulting in inactivation of the nucleic acid molecule containing the target sequence. As a result, the expression of mRNA containing the target sequence can be reduced.
  • an oligomeric molecule may be attached to a delivery moiety.
  • delivery moieties include glycoprotein receptors, galactoses, galactosamines, N-acetylgalactosamines, GalNAc groups, cholesterols, sterols, phytosterols, steroids, zoosterols, lanosterols, stigmastanols, dihydrolanosterols, zymosterols, zymostenols, desmosterols, and 7-dehydrocholesterol s.
  • this invention provides therapeutics for preventing, ameliorating, or treating a disease caused by Hepatitis B infection.
  • An active compound or molecule of this invention may be used in the prevention or treatment of a viral infection caused by Hepatitis B virus.
  • This invention provides structures, methods and compositions for oligomeric agents that incorporate the linker group monomers.
  • the oligomeric molecules of this invention can be used as active agents in formulations for gene silencing therapeutics targeted to HBV.
  • This invention provides a range of molecules that are useful for providing therapeutic effects because of their activity in regulating expression of a gene.
  • the molecules of this invention are structured to provide gene regulating or silencing activity in vitro and in vivo.
  • Embodiments of this invention can provide molecules for use as therapeutic agents against Hepatitis B infection.
  • the molecules can be used as active pharmaceutical ingredients in compositions for ameliorating, preventing or treating Hepatitis B infection.
  • an active molecule can be structured as an oligomer composed of monomers.
  • the oligomeric structures of this invention may contain one or more linker group monomers, along with certain nucleotides.
  • Molecules of this invention for treating Hepatitis B infection may act against any of the replication, maturation, growth, or transmission modalities of the Hepatitis B virus. By preventing the Hepatitis B virus from carrying out any one or more of its normal processes, the molecules of this invention can be used for ameliorating or treating Hepatitis B infection.
  • This invention can provide unexpectedly advantageous efficacy against HBV infection in a subject by simultaneously modulating all portions of the HBV genome.
  • inventive UNA oligomeric agents and compositions of this disclosure can inhibit expression of each of the HBV genes C, P, S, and X.
  • inventive UNA oligomeric agents and compositions of this disclosure can simultaneously inhibit expression of all genes of HBV, including genes C, P, S, and X.
  • inventive UNA oligomeric compositions of this disclosure can simultaneously inhibit expression of multiple genes of HBV, such as genes P and C, or P and S, or P and X.
  • inventive UNA oligomeric compositions of this disclosure can simultaneously inhibit expression of multiple genes of HBV, such as genes P, S and C, or P, X and S, or P, C and X.
  • the compounds of this invention can inhibit the small surface antigen HBsAg in vivo, regardless of the genomic source of HBsAg in the subject.
  • compounds and compositions of this invention can inhibit the HBV extracellular protein HBeAg, the X protein, and HBV viral polymerase.
  • a therapeutic molecule of this invention can be active in preventing or inhibiting a step of the replication cycle of hepatitis B virus.
  • Viral components of HBV can include a nucleocapsid, fully or partially double stranded DNA (relaxed circular, rcDNA), a polymerase, surface antigens, core proteins, a regulatory X-protein, and secreted proteins.
  • rcDNA fully or partially double stranded DNA
  • a therapeutic molecule of this invention can be active in preventing or inhibiting attachment of viral components to cell-associated proteoglycans.
  • Certain embodiments of this invention provide a therapeutic molecule that can be active in preventing or inhibiting binding of a viral component to a hepatocyte receptor.
  • a therapeutic molecule of this invention can be active in preventing or inhibiting entry of a viral component into a cell by endocytosis, or fusion of a viral component to a cell membrane.
  • a therapeutic molecule of this invention may be active in preventing or inhibiting release of a viral component into the cytoplasm of a cell.
  • a therapeutic molecule of this invention can be active in preventing or inhibiting internal cell transport of an HBV nucleocapsid.
  • aspects of this disclosure can provide a therapeutic molecule, which can be active in preventing or inhibiting release of HBV rcDNA in a cell.
  • a therapeutic molecule of this invention can be active in preventing or inhibiting operation of the viral polymerase.
  • Certain embodiments may provide a therapeutic molecule that can be active in preventing or inhibiting development of an HBV genomic DNA in a cell.
  • a therapeutic molecule of this invention can be active in preventing or inhibiting production of a viral RNA in a cell.
  • a therapeutic molecule of this invention may be active in preventing or inhibiting viral replication in a cell.
  • a therapeutic molecule may be active in preventing or inhibiting an HBV regulatory X-protein in a cell.
  • FIG. 1 Further aspects of this disclosure can provide a therapeutic molecule that be active in preventing or inhibiting translation or reverse transcription of a viral RNA in a cell.
  • a therapeutic molecule of this invention can be active in preventing or inhibiting maturation of a viral nucleocapsid in a cell.
  • linker group monomers can be unlocked nucleomonomers (UNA monomers), which are small organic molecules based on a propane-1,2,3-tri-yl-trisoxy structure as shown below:
  • UNA monomers unlocked nucleomonomers
  • R 1 and R 2 are H, and R 1 and R 2 can be phosphodiester linkages
  • Base can be a nucleobase
  • R 3 is a functional group described below.
  • the UNA monomer main atoms can be drawn in IUPAC notation as follows:
  • nucleobase examples include uracil, thymine, cytosine, 5-methylcytosine, adenine, guanine, inosine, and natural and non-natural nucleobase analogues.
  • a UNA monomer can be an internal monomer in an oligomer, where the UNA monomer is flanked by other monomers on both sides.
  • the UNA monomer can participate in base pairing when the oligomer is a duplex, for example, and there are other monomers with nucleobases in the duplex.
  • a UNA monomer can be a monomer in an overhang of an oligomer duplex, where the UNA monomer is flanked by other monomers on both sides. In this form, the UNA monomer does not participate in base pairing. Because the UNA monomers are flexible organic structures, unlike nucleotides, the overhang containing a UNA monomer will be a flexible terminator for the oligomer.
  • a UNA monomer can be a terminal monomer in an overhang of an oligomer, where the UNA monomer is attached to only one monomer at either the propane-1-yl position or the propane-3-yl position. In this form, the UNA monomer does not participate in base pairing. Because the UNA monomers are flexible organic structures, unlike nucleotides, the overhang containing a UNA monomer can be a flexible terminator for the oligomer.
  • a UNA monomer can be a flexible molecule
  • a UNA monomer as a terminal monomer can assume widely differing conformations.
  • An example of an energy minimized UNA monomer conformation as a terminal monomer attached at the propane-3-yl position is shown below.
  • UNA oligomers having a terminal UNA monomer are significantly different in structure from conventional nucleic acid agents, such as siRNAs.
  • siRNAs may require that terminal monomers or overhangs in a duplex be stabilized.
  • the conformability of a terminal UNA monomer can provide UNA oligomers with different properties.
  • a UNA oligomer can be a chain composed of UNA monomers, as well as various nucleotides that may be based on naturally-occurring nucleosides.
  • the functional group R 3 of a UNA monomer can be —OR 4 , —SR 4 , —NR 4 2 , —NH(C ⁇ O)R 4 , morpholino, morpholin-1-yl, piperazin-1-yl, or 4-alkanoyl-piperazin-1-yl, where R 4 is the same or different for each occurrence, and can be H, alkyl, a cholesterol, a lipid molecule, a polyamine, an amino acid, or a polypeptide.
  • the UNA monomers are organic molecules. UNA monomers are not nucleic acid monomers or nucleotides, nor are they naturally-occurring nucleosides or modified naturally-occurring nucleosides.
  • a UNA oligomer of this invention is a synthetic chain molecule.
  • a UNA oligomer of this invention is not a nucleic acid, nor an oligonucleotide.
  • a UNA monomer can be UNA-A (designated ⁇ ), UNA-U (designated ⁇ ), UNA-C(designated ⁇ hacek over (C) ⁇ ), and UNA-G (designated ⁇ hacek over (G) ⁇ ).
  • Designations that may be used herein include mA, mG, mC, and mU, which refer to the 2′-O-Methyl modified ribonucleotides.
  • Designations that may be used herein include lower case c and u, which refer to the 2′-O-methyl modified ribonucleotides.
  • Designations that may be used herein include dT, which refers to a 2′-deoxy T nucleotide.
  • X represents a UNA monomer
  • N represents any natural nucleotide monomer, or a modified nucleotide monomer.
  • the symbol Q represents a non-natural, modified, or chemically-modified nucleotide monomer.
  • the monomer can have any base attached.
  • the Q monomer can have any base attached that would be complementary to the monomer in the corresponding paired position in the other strand.
  • nucleic acid monomers include non-natural, modified, and chemically-modified nucleotides, including any such nucleotides known in the art.
  • non-natural, modified, and chemically-modified nucleotide monomers include any such nucleotides known in the art, for example, 2′-O-methyl ribonucleotides, 2′-O-methyl purine nucleotides, 2′-deoxy-2′-fluoro ribonucleotides, 2′-deoxy-2′-fluoro pyrimidine nucleotides, 2′-deoxy ribonucleotides, 2′-deoxy purine nucleotides, universal base nucleotides, 5-C-methyl-nucleotides, and inverted deoxyabasic monomer residues.
  • non-natural, modified, and chemically-modified nucleotide monomers include 3′-end stabilized nucleotides, 3′-glyceryl nucleotides, 3′-inverted abasic nucleotides, 3′-inverted thymidine, and L-thymidine.
  • non-natural, modified, and chemically-modified nucleotide monomers include locked nucleic acid nucleotides, 2′-O,4′-C-methylene-(D-ribofuranosyl) nucleotides, 2′-methoxyethoxy (MOE) nucleotides, 2′-methyl-thio-ethyl, 2′-deoxy-2′-fluoro nucleotides, and 2′-O-methyl nucleotides.
  • locked nucleic acid nucleotides 2′-O,4′-C-methylene-(D-ribofuranosyl) nucleotides
  • MOE methoxyethoxy
  • non-natural, modified, and chemically-modified nucleotide monomers examples include 2′-amino nucleotides, 2′-O-amino nucleotides, 2′-C-allyl nucleotides, and 2′-O-allyl nucleotides.
  • non-natural, modified, and chemically-modified nucleotide monomers include N 6 -methyladenosine nucleotides.
  • nucleotide monomers examples include nucleotide monomers with modified bases 5-(3-amino)propyluridine, 5-(2-mercapto)ethyluridine, 5-bromouridine; 8-bromoguanosine, or 7-deazaadenosine.
  • non-natural, modified, and chemically-modified nucleotide monomers include 2′-O-aminopropyl substituted nucleotides.
  • non-natural, modified, and chemically-modified nucleotide monomers include 2′-O-guanidinopropyl substituted nucleotides.
  • non-natural, modified, and chemically-modified nucleotide monomers examples include Pseudouridines.
  • non-natural, modified, and chemically-modified nucleotide monomers include replacing the 2′—OH group of a nucleotide with a 2′-R, a 2′-OR, a 2′-halogen, a 2′-SR, or a 2′-amino, 2′-azido, where R can be H, alkyl, fluorine-substituted alkyl, alkenyl, or alkynyl.
  • non-natural, modified, and chemically-modified nucleotide monomers include replacing the 2′—OH group of a nucleotide with a 2′-R or 2′-OR, where R can be CN, CF 3 , alkylamino, or aralkyl.
  • non-natural, modified, and chemically-modified nucleotide monomers include nucleotides with a modified sugar such as an F-HNA, an HNA, a CeNA, a bicyclic sugar, or an LNA.
  • a modified sugar such as an F-HNA, an HNA, a CeNA, a bicyclic sugar, or an LNA.
  • non-natural, modified, and chemically-modified nucleotide monomers examples include 2′-oxa-3′-aza-4′ a-carbanucleoside monomers, 3-hydroxymethyl-5-(1H-1,2,3-triazol)-isoxazolidine monomers, and 5′-triazolyl-2′-oxa-3′-aza-4′ a-carbanucleoside monomers.
  • modified nucleotides are given in Saenger, Principles of Nucleic Acid Structure, Springer-Verlag, 1984.
  • aspects of this invention can provide structures and compositions for UNA-containing oligomeric compounds.
  • the oligomeric agents may incorporate one or more UNA monomers.
  • Oligomeric molecules of this invention can be used as active agents in formulations for gene regulating or gene silencing therapeutics.
  • this invention provides oligomeric compounds having a structure that incorporates novel combinations of UNA monomers with certain natural nucleotides, non-natural nucleotides, modified nucleotides, or chemically-modified nucleotides.
  • the oligomeric compounds can be pharmacologically active molecules.
  • UNA oligomers of this invention can be used as active pharmaceutical ingredients for regulating gene expression, and in RNA interference methods, as well as antisense, RNA blocking, and micro-RNA strategies.
  • a UNA oligomer of this invention can have the structure of Formula I
  • L 1 is a linkage, n is from 19 to 29, and for each occurrence L 2 is a UNA linker group having the formula —C 1 —C 2 —C 3 —, where R is attached to C 2 and has the formula —OCH(CH 2 R 3 )R 5 , where R 3 is —OR 4 , —SR 4 , —NR 4 2 , —NH(C ⁇ O)R 4 , morpholino, morpholin-1-yl, piperazin-1-yl, or 4-alkanoyl-piperazin-1-yl, where R 4 is the same or different for each occurrence and is H, alkyl, a cholesterol, a lipid molecule, a polyamine, an amino acid, or a polypeptide, and where R 5 is a nucleobase, or L 2 (R) is a sugar such as a ribose and R is a nucleobase, or L 2 is a modified sugar such as a modified ribose and
  • a UNA oligomer of this invention can be a short chain molecule.
  • a UNA oligomer can be a duplex pair.
  • a UNA oligomer can have a first strand of the duplex and a second strand of the duplex, which is complementary to the first strand with respect to the nucleobases, although up to three mismatches can occur.
  • a UNA oligomer duplex can have overhangs.
  • the target of a UNA oligomer can be a target nucleic acid.
  • the target can be any mRNA of a subject.
  • a UNA oligomer can be active for gene silencing in RNA interference.
  • a UNA oligomer may comprise two strands that together provide a duplex.
  • the duplex may be composed of a first strand, which may also be referred to as a passenger strand or sense strand, and a second strand, which may also be referred to as a guide strand or antisense strand.
  • a UNA oligomer of this invention can have any number of phosphorothioate intermonomer linkages in any position in any strand, or in both strands of a duplex structure.
  • any one or more of the intermonomer linkages of a UNA oligomer can be a phosphodiester, a phosphorothioate including dithioates, a chiral phosphorothioate, and other chemically modified forms.
  • Examples of UNA oligomers of this invention include duplex pairs, which are in general complementary.
  • SEQ ID NO:1 can represent a first strand of a duplex and SEQ ID NO:2 can represent a second strand of the duplex, which is complementary to the first strand.
  • N in the first strand can represent any nucleotide that is complementary to the monomer in the corresponding position in the second strand.
  • Example UNA oligomers of this disclosure are shown with 2-monomer length overhangs, although overhangs of from 1 to 8 monomers, or longer, can be used.
  • the symbol “X” in a strand or oligomer represents a UNA monomer.
  • the monomer can have any base attached.
  • the UNA monomer can have any base attached that would be complementary to the monomer in the corresponding paired position in the other strand.
  • the terminal position has a 1-end, according to the positional numbering shown above, instead of a 5′-end as for a nucleotide, or the terminal position has a 3-end, according to the positional numbering shown above, instead of a 3′-end as for a nucleotide.
  • the UNA oligomer terminates in a UNA monomer
  • SEQ ID NO: 1 1-X ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N-N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N-X ⁇ X-3
  • SEQ ID NO: 2 3-X ⁇ X ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N ⁇ N-N ⁇ N ⁇ X ⁇ X ⁇ X ⁇ X ⁇ X ⁇ N-5′ has a UNA monomer 1-end on the first strand, a UNA monomer 3-end on the first strand, a UNA monomer 3-end on the second strand, and a nucleotide 5′-end on the second strand.
  • Complementarity of strands can involve mismatches.
  • complementarity of strands can include one to three, or more, mismatches.
  • a UNA oligomer of this invention can have one or more UNA monomers at the 1-end of the first strand, and one or more UNA monomers at the 3-end of the first strand.
  • a UNA oligomer of this invention can have one or more UNA monomers at the 3-end of the second strand.
  • a duplex UNA oligomer of this invention can have one or more UNA monomers at the 1-end of the first strand, one or more UNA monomers at the 3-end of the first strand, and one or more UNA monomers at the 3-end of the second strand.
  • a UNA oligomer of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length.
  • a UNA oligomer of this invention may have a first strand that is 19-23 monomers in length.
  • a UNA oligomer of this invention may have a duplex region that is 19-21 monomers in length.
  • a UNA oligomer of this invention may have a second strand that is 19-23 monomers in length.
  • a UNA oligomer of this invention may have a first strand that is 19 monomers in length, and a second strand that is 21 monomers in length.
  • a UNA oligomer of this invention may have a first strand that is 20 monomers in length, and a second strand that is 21 monomers in length.
  • a UNA oligomer of this invention may have a first strand that is 21 monomers in length, and a second strand that is 21 monomers in length.
  • a UNA oligomer of this invention may have a first strand that is 22 monomers in length, and a second strand that is 21 monomers in length.
  • a UNA oligomer of this invention for inhibiting gene expression can have a first strand and a second strand, each of the strands being 19-29 monomers in length.
  • the monomers can be UNA monomers and nucleic acid nucleoside monomers.
  • the oligomer can have a duplex structure of from 14 to 29 monomers in length.
  • the UNA oligomer can be targeted to a target gene and can exhibit reduced off-target effects as compared to a conventional siRNA.
  • a UNA oligomer of this invention can have a first strand and a second strand, each of the strands being 19-23 monomers in length.
  • the UNA oligomer may have a blunt end, or may have one or more overhangs.
  • the first and second strands may be connected with a connecting oligomer in between the strands, and form a duplex region with a connecting loop at one end.
  • an overhang can be one or two monomers in length.
  • an overhang can contain one or more UNA monomers, natural nucleotides, non-natural nucleotides, modified nucleotides, or chemically-modified nucleotides, and combinations thereof.
  • Examples of an overhang can contain one or more deoxythymidine nucleotides, 2′-O-methyl nucleotides, inverted abasic monomers, inverted thymidine monomers, L-thymidine monomers, or glyceryl nucleotides.
  • a UNA oligomer can mediate cleavage of a target nucleic acid in a cell.
  • the second strand of the UNA oligomer at least a portion of which can be complementary to the target nucleic acid, can act as a guide strand that can hybridize to the target nucleic acid.
  • the second strand can be incorporated into an RNA Induced Silencing Complex (RISC).
  • RISC RNA Induced Silencing Complex
  • a UNA oligomer of this disclosure may comprise naturally-occurring nucleic acid nucleotides, and modifications thereof that are compatible with gene silencing activity.
  • a UNA oligomer is a double stranded construct molecule that is able to inhibit gene expression.
  • strand refers to a single, contiguous chain of monomers, the chain having any number of internal monomers and two end monomers, where each end monomer is attached to one internal monomer on one side, and is not attached to a monomer on the other side, so that it ends the chain.
  • the monomers of a UNA oligomer may be attached via phosphodiester linkages, phosphorothioate linkages, gapped linkages, and other variations.
  • a UNA oligomer can include mismatches in complementarity between the first and second strands.
  • a UNA oligomer may have 1, or 2, or 3 mismatches. The mismatches may occur at any position in the duplex region.
  • the target of a UNA oligomer can be a target nucleic acid of a target gene.
  • a UNA oligomer may have one or two overhangs outside the duplex region.
  • the overhangs can be an unpaired portion at the end of the first strand or second strand.
  • the lengths of the overhang portions of the first and second strands can be the same or different.
  • a UNA oligomer may have at least one blunt end.
  • a blunt end does not have an overhang portion, and the duplex region at a blunt end terminates at the same position for both the first and second strands.
  • a UNA oligomer can be RISC length, which means that it has a duplex length of less than 25 base pairs.
  • a UNA oligomer can be a single strand that folds upon itself and hybridizes to itself to form a double stranded region having a connecting loop at the end of the double stranded region.
  • An oligomeric compound of this invention may have any one of the structures shown in Tables 1 to 13.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is less than twenty.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is less than twelve.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is less than ten.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is less than eight.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is from 1 to 20.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is from 1 to 15.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the number of Q monomers is from 1 to 9.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is less than twenty.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is less than twelve.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is less than ten.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is less than eight.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is from 1 to 20.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is from 1 to 15.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a 2′-O-Methyl modified ribonucleotide, and where the number of 2′-O-Methyl modified ribonucleotides is from 1 to 9.
  • an oligomeric compound of this invention may have a first strand and a second strand, each of the strands independently being 19-23 monomers in length, where any monomer that is not a UNA monomer can be a Q monomer, and where the oligomeric compound does not contain fluorine.
  • Embodiments of this invention advantageously provide oligomeric compounds, which are active agents against HBV and do not contain fluorine.
  • Methods of this invention include the treatment and/or prevention of HBV disease in a subject.
  • a subject can be a mammalian subject, including a human subject.
  • Ref Pos refers to reference position, which is the numerical position of a reference nucleotide in an HBV genome.
  • the reference position is the position that corresponds target-wise to the 5′ end of the sense strand of the oligomeric compound of this invention.
  • the reference positions are numerical nucleotide positions based on a reference genome, which as used herein is HBV Genotype A2, Accession No. HE974376.
  • a reference position number by itself refers to one sequence from the reference genome, and each sequence can be used in an oligomeric compound of this invention.
  • Table 14 shows genomic positions for the HBV reference genome.
  • FIG. 1 a map of HBV protein coding regions and selected transcripts for the reference genome HE974376. Nucleotide position 1/3221 is designated at the top. Further designations are as follows: pre-S1, large HBsAg; pre-S2, medium HBsAg; S, HBsAg; P, polymerase; X, HBx protein; pre-C, pre-core/HBeAg; C, HB core Ag. The 2.4 kb, 2.1 kb, and 0.7 kb transcripts coding for the pre-S1/pre-S2/S, as well as the transcript coding the X protein are shown.
  • the pre-Core/HBeAg protein is generated from a long, 3.5 kb transcript (not shown) originating at position ⁇ 1700, while the core and polymerase proteins and the pre-genomic RNA used as a template for viral replication are generated from a ⁇ 200 nt shorter transcript.
  • inventive oligomers of this disclosure may target the long transcript coding for HBV core and polymerase proteins.
  • An oligomeric compound of this invention can be formed having a first strand and a second strand each being 21 monomers in length.
  • the first strand can have 19 contiguous monomers with a sequence of attached bases shown in Table 15 (sense), and two additional overhang monomers on the 3′ end.
  • the second strand can have 19 contiguous monomers with a sequence of attached bases shown in Table 15 (antisense), and two additional overhang monomers on the 3′ end.
  • the overhang monomers can be any of NN, QQ, XX, NX, NQ, XN, XQ, QN, and QX.
  • XQ can be UNA-U/mU, or UNA-U/*/dT.
  • An oligomeric compound of this invention can be composed of monomers.
  • the monomers can have attached bases.
  • An oligomeric compound of this invention can have a sequence of attached bases.
  • the sequences of bases shown in Table 15 do not indicate to which monomer each of the bases in the sequence is attached. Thus, each sequence shown in Table 15 refers to a large number of small molecules, each of which is composed of UNA monomers, as well as nucleic acid monomers.
  • an oligomeric compound of this invention can be described by a sequence of attached bases, for example as shown in Table 15, and being substituted forms thereof.
  • substituted forms include differently substituted UNA monomers, as well as differently substituted or modified nucleic acid monomers, as are further described herein.
  • one or more of three monomers at each end of each strand can be connected by a phosphorothioate, a chiral phosphorothioate, or a phosphorodithioate linkage.
  • a compound may have one phosphorothioate linkage between two monomers at the 5′ end of the first strand, one phosphorothioate linkage between two monomers at the 3′ end of the first strand, one phosphorothioate linkage between monomers at the second and third positions from the 3′ end of the first strand, and one phosphorothioate linkage between two monomers at the 3′ end of the second strand.
  • a compound may have two or three phosphorothioate linkages at the 5′ end of the first strand, two or three phosphorothioate linkages at the 3′ end of the first strand, and one phosphorothioate linkage at the 3′ end of the second strand.
  • a compound may have one to three phosphorothioate linkages at the 5′ end of the first strand, two or three phosphorothioate linkages at the 3′ end of the first strand, two phosphorothioate linkages at the 5′ end of the second strand, and two phosphorothioate linkages at the 3′ end of the second strand.
  • a compound may have a deoxythymidine nucleotide at the 3′ end of the first strand, at the 3′ end of the second strand, or at both the 3′ end of the first strand and the 3′ end of the second strand.
  • a compound may contain one to five UNA monomers.
  • a compound may contain three UNA monomers.
  • a compound may contain a UNA monomer at the 1-end of the first strand (5′ end), a UNA monomer at the 3-end of the first strand (3′ end), and a UNA monomer at the second position from the 3′ end of the second strand.
  • a compound may contain a UNA monomer at any one or more of positions 2 to 8 from the 5′ end of the second strand (seed region).
  • An oligomeric compound of this invention can be formed having a first strand and a second strand each being 21 monomers in length.
  • the first strand can have 19 contiguous monomers with a sequence of attached bases shown in Table 16 (sense), and two additional overhang monomers on the 3′ end.
  • the second strand can have 19 contiguous monomers with a sequence of attached bases shown in Table 16 (antisense), and two additional overhang monomers on the 3′ end.
  • the overhang monomers can be any of NN, QQ, XX, NX, NQ, XN, XQ, QN, and QX.
  • XQ can be UNA-U/mU, or UNA-U/*/dT.
  • Embodiments of this invention can provide oligomeric molecules that are active agents targeted to HBV.
  • Embodiments of this invention can provide oligomeric molecules that are active agents targeted to HBV.
  • Embodiments of this invention can provide oligomeric molecules that are active agents targeted to HBV.
  • Embodiments of this invention can provide oligomeric molecules that are active agents targeted to HBV.
  • rN refers to N, which is a ribonucleotide
  • mN refers to a chemically-modified 2′-OMe ribonucleotide
  • an asterisk * between characters refers to a phosphorothioate linkage
  • dN refers to a deoxyribonucleotide
  • f refers to a 2′-deoxy-2′-fluoro ribonucleotide.
  • Embodiments of this invention can provide compositions of oligomeric molecules that are active agents targeted to HBV.
  • a composition for use against HBV viral infection can provide targeting for suppressing multiple viral gene products.
  • ORF open reading frames
  • a composition of this invention may contain an oligomeric compound targeted to an HBV genomic transcript or ORF for HBsAg.
  • these embodiments can inhibit expression of HBsAg, regardless of the location of the HBV genomic DNA.
  • compositions may contain an oligomeric compound targeted to an HBV genomic transcript or ORF for HBeAg.
  • compositions may contain an oligomeric compound targeted to an HBV genomic transcript or ORF for X protein.
  • composition may contain an oligomeric compound targeted to an HBV genomic transcript or ORF for DNA polymerase (P).
  • P DNA polymerase
  • a composition may contain an oligomeric compound targeted to a conserved HBV genomic region of the transcripts or open reading frames from genes X, S, and C.
  • a composition may contain an oligomeric compound targeted to a conserved HBV genomic region of the transcripts or open reading frames from genes X, S, C and P.
  • composition of this invention includes a dyad of oligomeric compounds as the active agents targeted to HBV.
  • dyad compositions include a composition containing a compound with a reference position in the range 1403 to 1623, and a compound with a reference position in the range 155 to 550.
  • dyad compositions include a composition containing a compound with a reference position in the range 1575 to 1581, and a compound with a reference position in the range 245 to 414.
  • dyad compositions include a composition containing a compound with a reference position in the range 1525 to 1604, and a compound with a reference position in the range 374 to 414.
  • dyad compositions include a composition containing a compound with a reference position in the range 1525 to 1604, and a compound with a reference position in the range 1776 to 1818.
  • dyad compositions include a composition containing a compound with a reference position in the range 374 to 414, and a compound with a reference position in the range 1776 to 1782.
  • Examples of dyad compositions include a composition containing a compound with the reference position 1578 and a compound with the reference position 380.
  • Examples of dyad compositions include a composition containing a compound with the reference position 1578 and a compound with the reference position 376 or 411.
  • dyad compositions include compositions containing compounds with the reference positions 1575 and 376, 1575 and 380, 1575 and 511, 1581 and 376, 1581 and 380, as well as 1581 and 411.
  • dyad compositions include compositions containing a compound with the reference position 1578 and a compound with the reference position 1777.
  • dyad compositions include compositions containing compounds with the reference positions 1578 and 1780, or 1578 and 1782, or 1575 and 1777, or 1575 and 1780, or 1575 and 1782, or 1581 and 1777, or 1581 and 1780, or 1581 and 1782, or 1576 and 1777, or 1576 and 1780, or 1576 and 1782.
  • a dyad composition may contain the compounds 1578 and 380 shown in Table 22.
  • composition of this invention includes triads of oligomeric compounds as the active agents targeted to HBV.
  • triad compositions include a composition containing a compound with a reference position in the range 1403 to 1623, a compound with a reference position in the range 155 to 550, and a compound with a reference position in the range 1624 to 1930.
  • triad compositions include a composition containing a compound with a reference position in the range 1525 to 1582, a compound with a reference position in the range 245 to 414, and a compound with a reference position in the range 1777 to 1818.
  • triad compositions include a composition containing a compound with a reference position in the range 1525 to 1604, a compound with a reference position in the range 374 to 414, and a compound with a reference position in the range 1776 to 1782.
  • triad compositions include a composition containing a compound with a reference position in the range 1525 to 1582, a compound with a reference position in the range 374 to 414, and a compound with a reference position in the range 1776 to 1782.
  • triad compositions include a composition containing a compound with the reference position 1578, a compound with the reference position 380, and a compound with the reference position 1777.
  • triad compositions include a composition containing a compound with the reference position 1576, a compound with the reference position 380, and a compound with the reference position 1777.
  • triad compositions include a composition containing a compound with the reference position 1575, a compound with the reference position 380, and a compound with the reference position 1777.
  • triad compositions include a composition containing a compound with the reference position 1578, a compound with the reference position 1777, and a compound with the reference position 376 or 411.
  • triad compositions include a composition containing a compound with the reference position 1578, a compound with the reference position 1780 or 1782, and a compound with the reference position 376 or 411.
  • a triad composition may contain the compounds 1578, 380 and 1777 shown in Table 23.
  • rN refers to N, which is a ribonucleotide
  • mN refers to a chemically-modified 2′-OMe ribonucleotide
  • an * between characters refers to a phosphorothioate linkage
  • dN refers to a deoxyribonucleotide.
  • Methods of this invention include the treatment and prevention of various diseases in mammalian subjects.
  • a subject can be a human or mammal.
  • a subject in need of treatment or prevention can be administered an effective amount of an oligomeric compound of this invention.
  • An effective amount of an oligomeric compound of this invention can be a dose ranging from 0.001 mg/kg to 50.0 mg/kg.
  • target mRNA expression can be reduced in a subject for at least 5 days. In certain embodiments, target mRNA expression can be reduced in a subject for at least 10 days, or 15 days.
  • the administration of an oligomeric compound may not result in an inflammatory response.
  • this invention includes methods for inhibiting expression of a target gene in a cell, by treating the cell with an oligomeric compound of this invention.
  • this invention includes methods for inhibiting expression of a target gene in a mammal, by administering to the mammal a composition containing an oligomeric compound of this invention.
  • this invention provides pharmaceutical compositions containing an oligomeric compound and a pharmaceutically acceptable carrier.
  • a pharmaceutical composition can be capable of local or systemic administration.
  • a pharmaceutical composition can be capable of any modality of administration.
  • the administration can be intravenous, subcutaneous, pulmonary, intramuscular, intraperitoneal, dermal, oral, or nasal administration.
  • Embodiments of this invention include pharmaceutical compositions containing an oligomeric compound in a lipid formulation.
  • a pharmaceutical composition may comprise one or more lipids selected from cationic lipids, anionic lipids, sterols, pegylated lipids, and any combination of the foregoing.
  • a pharmaceutical composition can be substantially free of liposomes.
  • a pharmaceutical composition can include liposomes or nanoparticles.
  • lipids and lipid compositions for delivery of an active molecule of this invention are given in WO/2015/074085, which is hereby incorporated by reference in its entirety.
  • a pharmaceutical composition can contain an oligomeric compound within a viral or bacterial vector.
  • a pharmaceutical composition of this disclosure may include carriers, diluents or excipients as are known in the art. Examples of pharmaceutical compositions are described, for example, in Remington's Pharmaceutical Sciences , Mack Publishing Co. (A.R. Gennaro ed. 1985).
  • excipients for a pharmaceutical composition include antioxidants, suspending agents, dispersing agents, preservatives, buffering agents, tonicity agents, and surfactants.
  • Luciferase-based reporter plasmid was constructed based on psiCHECKTM2 vector (Promega, Madison, Wis.). Reporter p(1-20) was generated with oligonucleotides containing the sequence from position 1 through 2500 relative to Eco RI digestion site cloned into the multiple cloning region downstream of the stop codon of the SV40 promoted Renilla luciferase gene in psiCHECKTM2, which made the expression of Renilla luciferase gene under the regulation of the artificial 3′UTR sequence. Renilla luciferase activity was then used as an indicator of the effect of the artificial 3′UTR on transcript stability and translation efficiency.
  • the psiCHECKTM-2 Vector also contained a constitutively expressed Firefly luciferase gene, which served as an internal control to normalize transfection efficiency.
  • HepB3 cells American Type Culture Collection
  • the cells were incubated at 37° C. in 100 ⁇ l of DMEM (Life Technologies, Carlsbad, Calif.) supplemented with 0.1 mM nonessential amino acids and 10% FBS (Life Technologies, Carlsbad, Calif.).
  • the culture medium was changed to 90 ⁇ l of fresh medium just before the transfection.
  • the reporter plasmid and UNA Oligomer were co-transfected with transfection reagent, LipofectamineTM 3000 (Life Technologies, Carlsbad, Calif.) was used to transfect reporter plasmid (100 ng) and a various amount of UNA Oligomer together with P3000 into the cells according to manufacturer's instruction.
  • Dual-Luciferase Reporter Assay System (DLR assay system, Promega, Madison, Wis.) was used to perform dual-reporter assays on psiCHECK2 based reporter systems. Twenty-four hours after transfection, the cells were washed gently with phosphate buffered saline once. A 50 ⁇ l well of Passive Lysis Buffer (Promega, Madison, Wis.) was added to the cells and incubated with gentle rocking for 20 min at room temperature. Luciferase activities were measured using Cytation 3 imaging reader (BioTek, Winooski, Vt.) and the effect of the UNA Oligomer on reporter expression was calculated based on ratio of Renilla/Firefly to normalize cell number and transfection efficiency.
  • DLR assay system Promega, Madison, Wis.
  • the HBV inhibitory effect of UNA oligomers was observed with a psiCHECK2 assay. At 1 nM concentration for 6 days, the percent inhibition of target expression for each of the UNA oligomeric compounds in Table 19 designated as having Reference Position 1578 was determined to be from 77% to 97%. Thus, all of the UNA oligomeric compounds in Table 19 having Reference Position 1578 were operable for silencing target expression.
  • the HBV inhibitory effect of UNA oligomers was observed with a psiCHECK2 assay. At 1 nM concentration for 6 days, the percent inhibition of target expression for each of the UNA oligomeric compounds in Table 19 designated as having Reference Position 1777 was determined to be from 77% to 92%. Thus, all of the UNA oligomeric compounds in Table 19 having Reference Position 1777 were operable for silencing target expression.
  • the HBV inhibitory effect of UNA oligomers was observed with a psiCHECK2 assay. At 1 nM concentration for 6 days, the percent inhibition of target expression for each of the UNA oligomeric compounds in Table 19 designated as having Reference Position 380 was determined to be from 87% to 94%. Thus, all of the UNA oligomeric compounds in Table 19 having Reference Position 380 were operable for silencing target expression.
  • UNA oligomeric compounds of this invention were operable for modulating HBV target expression.
  • the UNA oligomeric compounds of this invention exhibited picomolar activity in vitro for inhibiting target expression.
  • the UNA oligomeric compounds of this invention exhibited surprisingly high activity in vitro of about IC50 ⁇ 200 pM for inhibiting target expression.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a humanized PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulations, ⁇ 1 and ⁇ 2.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • mice were treated with 3 mg/kg on day 0, then 5 mg/kg on day 4, then 10 mg/kg on day 8.
  • compositions in FIG. 2 and Tables 26 and 27 were UNA oligomer triad composition (1777 (SEQ ID NO:1005 and 1006), 380 (SEQ ID NO:973 and 974), 1576 (SEQ ID NO:989 and 990)).
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the treatment with UNA oligomer triad composition (1576, 380, 177) was significantly superior to UNA oligomer 1576.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • mice were administered every 4 days, up to day 40, and viral endpoints were monitored every 4 days through day 44.
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • Serum viral endpoints were monitored up to 15 days after the single injection.
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the AAV-HBV mouse model is a robust model for investigating HBV infection, and can provide direct clinical pertinence for drug efficacy and potency.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • mice were treated with 3 mg/kg on day 0, then 5 mg/kg on day 4, then 10 mg/kg on day 8.
  • Serum viral endpoints were monitored 15 days before, and at least 22 days after treatment.
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were co-formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • mice were treated with 3 mg/kg on day 0, then 5 mg/kg on day 4, then 10 mg/kg on day 8.
  • Serum viral endpoints were monitored up to day 12 after treatment.
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in an AAV-HBV mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the AAV-HBV mouse model is a robust model for investigating HBV infection, and can provide direct clinical pertinence for drug efficacy and potency.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into C57Bl/6 mice with active HBV replication after AAV-mediated delivery of a recombinant HBV genome to the liver.
  • mice were treated with 3 mg/kg on day 0, then 5 mg/kg on day 4, then 10 mg/kg on day 8.
  • Serum viral endpoints were monitored 15 days before, and at least 22 days after treatment.
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect of UNA oligomers was observed with a psiCHECK2 assay.
  • the percent inhibition of target expression for UNA oligomeric compounds containing one or more 2′-deoxy-2′-fluoro ribonucleotides was measured.
  • UNA oligomeric compounds exhibited at least 87% inhibition of target expression at 10 nM.
  • the UNA oligomers of this invention demonstrated advantageous HBV inhibition efficacy in vitro.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • the UNA oligomers of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • Serum HBsAg (% control) (normalized to hAlb) % % % % UNA oligomer Inhibition Inhibition Inhibition Inhibition composition
  • Day 5 Day 10
  • Day 15 Day 20
  • Ref. Pos. 3.3 nM 3.3 nM 3.3 nM 3.3 nM 3.3 nM 380/1777/1575 82.0 67.0 39.9 28.0 380/1777/1578 82.0 70.0 47.3 33.2 380/1777/1576 79.0 64.0 44.8 29.1
  • compositions in Table 30 were:
  • UNA oligomer triad composition (1777 (SEQ ID NO:1005 and 1006), 380 (SEQ ID NO:973 and 974), 1575 (SEQ ID NO:987 and 988)); UNA oligomer triad composition (1777 (SEQ ID NO:1005 and 1006), 380 (SEQ ID NO:973 and 974), 1578 (SEQ ID NO:993 and 994)); and UNA oligomer triad composition (1777 (SEQ ID NO:1005 and 1006), 380 (SEQ ID NO:973 and 974), 1576 (SEQ ID NO:989 and 990)).
  • the triad UNA oligomer compositions of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • Serum HBsAg (% control) (normalized to hAlb) % % % % UNA oligomer Inhibition Inhibition Inhibition Inhibition composition
  • Geno- Day 5 Day 10 Day 5 Day 10 (Ref. Pos.) type 3 nM 3 nM 15 nM 15 nM 380/1777/1578 Ae 79.2 71.0 87.5 79.0 380/1777/1578 Bj 75.4 62.2 85.0 79.0 380/1777/1578 C — 68.8 — 82.8 380/1777/1578 D 80.7 68.9 88.5 81.4
  • composition in Table 31 was UNA oligomer triad composition (1777 (SEQ ID NO:1005 and 1006), 380 (SEQ ID NO:973 and 974), 1578 (SEQ ID NO:993 and 994)).
  • the triad UNA oligomer compositions of this invention demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo over a range of genotypes.
  • the HBV inhibitory effect in vivo for UNA oligomers was observed in a PXB Mouse model of HBV infection.
  • the UNA oligomers of this invention exhibited profound reduction of HBV serum infection parameters in vivo with phosphorothioate linkages present.
  • the UNA oligomers were contained in lipid nanoparticle formulation.
  • the UNA oligomers were formulated or co-formulated in lipid nanoparticles and injected intravenously into HBV-infected Phoenix Bio (PXB) mice.
  • the mice were Genotype: cDNA-uPA wild/+ /SCID [cDNA-uPA wild/+ : B6;129SvEv-Plau, SCID: C.B-17/Icr-scid/scid Jcl] containing human hepatocytes with an estimated replacement index of 70% or more.
  • the UNA oligomers of this invention with phosphorothioate linkages demonstrated significant and unexpectedly advantageous HBV inhibition efficacy in vivo with longer duration (Day 15 to Day 20).
  • the phosphorothioate linkages were as follows: one phosphorothioate linkage between two monomers at the 5′ end of the first strand, one phosphorothioate linkage between two monomers at the 3′ end of the first strand, one phosphorothioate linkage between monomers at the second and third positions from the 3′ end of the first strand, and one phosphorothioate linkage between two monomers at the 3′ end of the second strand.

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